{"id":103772,"date":"2019-02-19T00:00:50","date_gmt":"2019-05-17T08:53:20","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/103772\/"},"modified":"2019-05-17T16:53:22","modified_gmt":"2019-05-17T08:53:22","slug":"0abc7010c5","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/103772\/","title":{"rendered":"\u75c5\u6bd2\u7c7b\u751f\u7269\u5236\u54c1\u7684\u8fde\u7eed\u751f\u4ea7"},"content":{"rendered":"<div class=\"bpp-post-content\">\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word 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!important;\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);text-align: center;box-sizing: border-box !important;word-wrap: break-word !important;\"><img class=\"\"       style=\"box-sizing: border-box !important;word-wrap: break-word !important;width: 251px !important;visibility: visible !important;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/8-1558083201.png\" alt=\"\u75c5\u6bd2\u7c7b\u751f\u7269\u5236\u54c1\u7684\u8fde\u7eed\u751f\u4ea7\" title=\"\u75c5\u6bd2\u7c7b\u751f\u7269\u5236\u54c1\u7684\u8fde\u7eed\u751f\u4ea7\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 12px;box-sizing: border-box !important;word-wrap: break-word !important;\"><em style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\u4f7f\u7528\u914d\u7f6e\u4ea4\u66ff\u5f0f\u5207\u5411\u6d41\uff08ATF\uff09\u8fc7\u6ee4\u7684\u6405\u62cc\u7f50\u751f\u7269\u53cd\u5e94\u5668\u8fdb\u884c\u704c\u6d41\u57f9\u517b\uff0c\u4ee5\u8fdb\u884c\u75c5\u6bd2\u9897\u7c92\u8fde\u7eed\u751f\u4ea7\u65f6\u7684\u53cd\u5e94\u5668\u914d\u7f6e<em style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\uff08S.Gutierrez-Granados, et al. 2018\uff09\u3002<\/em><\/em><\/span><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word 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style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);text-align: center;box-sizing: border-box !important;word-wrap: break-word !important;\"><img class=\"\"       style=\"box-sizing: border-box !important;word-wrap: break-word !important;width: 448px !important;visibility: visible !important;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/4-1558083201.jpeg\" alt=\"\u75c5\u6bd2\u7c7b\u751f\u7269\u5236\u54c1\u7684\u8fde\u7eed\u751f\u4ea7\" title=\"\u75c5\u6bd2\u7c7b\u751f\u7269\u5236\u54c1\u7684\u8fde\u7eed\u751f\u4ea7\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><em style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 12px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u5e38\u89c1\u751f\u4ea7\u65b9\u5f0f\uff08\u77ac\u65f6\u8f6c\u67d3\u3001\u75c5\u6bd2\u611f\u67d3\u53ca\u7a33\u5b9a\u7ec6\u80de\u7cfb\uff09\u793a\u610f\u56fe\uff08S.Gutierrez-Granados, et al. 2018\uff09\u3002<\/span><\/em><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 14px;background-color: rgb(136, 136, 136);color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\">\u8fde\u7eed\u57f9\u517b\u4e3a\u57fa\u4e8e\u7ec6\u80de\u7684\u75c5\u6bd2\u6027\u75ab\u82d7\u751f\u4ea7\u5de5\u827a\u7684\u4f18\u5316\u63d0\u4f9b\u4e86\u89e3\u51b3\u65b9\u6848\u3002<\/span><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u5df2\u6709\u5173\u4e8e\u4e0d\u540c\u8fde\u7eed\u57f9\u517b\u65b9\u6cd5\u7684\u62a5\u5bfc\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 14px;color: rgb(22, 87, 136);box-sizing: border-box !important;word-wrap: break-word !important;\">\u7528\u4e8e\u75ab\u82d7\u751f\u4ea7\u7684\u704c\u6d41\u57f9\u517b<\/span><\/strong><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u4e0e\u91cd\u7ec4\u86cb\u767d\u548c\u5355\u514b\u9686\u6297\u4f53\u4e0d\u540c\u7684\u662f\uff0c\u75c5\u6bd2\u7ed3\u6784\u7684\u590d\u6742\u6027\u59a8\u788d\u4e86\u5bbf\u4e3b\u7ec6\u80de\u751f\u4ea7\u5927\u91cf\u75c5\u6bd2\u7684\u80fd\u529b\uff0c\u6240\u4ee5\uff0c\u7ec6\u80de\u7279\u5f02\u6027\u75c5\u6bd2\u4ea7\u7387\u901a\u5e38\u8f83\u4f4e\u3002\u51fa\u4e8e\u6b64\u539f\u56e0\uff0c\u4e3a\u7ec6\u80de\u8fde\u7eed\u63d0\u4f9b\u65b0\u9c9c\u7684\u8425\u517b\u7269\u8d28\uff0c\u540c\u65f6\u53bb\u9664\u4ee3\u8c22\u526f\u4ea7\u7269\uff0c\u6709\u52a9\u4e8e\u63d0\u9ad8\u7ec6\u80de\u6240\u80fd\u751f\u4ea7\u7684\u75c5\u6bd2\u6570\u91cf\u3002Nikolay\u7b49\u5728\u6279\u6b21\u6447\u74f6\u4e2d\u4ee5BHK-21\u60ac\u6d6e\u7ec6\u80de\u751f\u4ea7\u5be8\u5361\u75c5\u6bd2\uff0c\u6bd4\u4f7f\u7528\u8d34\u58c1Vero\u7ec6\u80de\u7684\u6807\u51c6\u751f\u4ea7\uff0c\u4ea7\u7387\u8981\u4f4e\uff089&#215;10^3 PFU\/mL vs. ~10^7PFU\/mL\uff09\u3002\u4e3a\u63d0\u9ad8\u6ef4\u5ea6\uff0c\u4f5c\u8005\u4f7f\u7528ATF\u7cfb\u7edf\u8fdb\u884c\u4e86\u704c\u6d41\u57f9\u517b\u3002\u611f\u67d3\u524d\uff0c\u901a\u8fc7\u5c06\u704c\u6d41\u901f\u7387\u4ece0.15VVD\u63d0\u5347\u81f30.42VVD\uff0c\u7ec6\u80de\u751f\u957f\u81f312&#215;10^6cells\/mL\u3002\u611f\u67d3\u540e\uff0c\u704c\u6d41\u7ef4\u63016\u5929\uff0c\u8fbe\u52303.9&#215;10^7PFU\/mL\u7684\u751f\u4ea7\u7ed3\u679c\u3002\u5c3d\u7ba1\u76f8\u6bd4\u8d34\u58c1\u7ec6\u80de\u57f9\u517b\uff0c\u60ac\u6d6e\u7ec6\u80de\u7684\u7279\u5f02\u6027\u75c5\u6bd2\u4ea7\u7387\u4ecd\u8f83\u4f4e\uff0c\u4f46\u662f<\/span><span style=\"max-width: 100%;font-size: 14px;color: rgb(255, 255, 255);background-color: rgb(136, 136, 136);box-sizing: border-box !important;word-wrap: break-word !important;\">\u5efa\u7acb\u4e86\u4e00\u79cd\u7528\u4e8e\u5be8\u5361\u75c5\u6bd2\u7684\u4ea7\u91cf\u8db3\u591f\u7684\u53ef\u653e\u5927\u751f\u4ea7\u5de5\u827a<\/span><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">Cervera\u7b49\u5f00\u53d1\u4e86\u4e00\u79cd\u53ef\u5f3a\u5316\u4eba\u514d\u75ab\u7f3a\u9677\u75c5\u6bd2\uff08HIV\uff09VLP\u751f\u4ea7\u7684\u65b0\u65b9\u6cd5\uff0c\u4f7f\u7528\u91cd\u590d\u77ac\u65f6\u8f6c\u67d3\u548c\u57f9\u517b\u57fa\u7f6e\u6362\u7684HEK293\u7ec6\u80de\u57f9\u517b\uff0c\u547d\u540d\u4e3aExtended Gene Expression\uff08EGE\uff09\u3002\u8f6c\u67d3\u540e\uff0c\u6bcf48\u5c0f\u65f6\u7f6e\u6362\u57f9\u517b\u57fa\uff0c\u76f8\u6bd472\u5c0f\u65f6\u7684\u6279\u6b21\u57f9\u517b\uff0c\u86cb\u767d\u8d28\u751f\u4ea7\u53ef\u63d0\u9ad88\u500d\u3002\u800c\u7ed3\u5408\u4e24\u6b21\u518d\u8f6c\u67d3\uff0c\u53ef\u63d0\u9ad812\u500d\u3002\u5728\u914d\u6709\u704c\u6d41\u76841.3L\u751f\u7269\u53cd\u5e94\u5668\u4e0a\u6210\u529f\u64cd\u4f5c\u4e86\u8fd9\u79cd\u65b9\u6cd5\uff0c\u4ee50.5VVD\u7684\u901f\u7387\u7f6e\u6362\u57f9\u517b\u57fa\uff0c\u4f7f\u7528\u8fde\u7eed\u704c\u6d41\uff0c\u7ec6\u80de\u53ef\u751f\u957f\u81f3\u66f4\u9ad8\u7684\u7ec6\u80de\u5bc6\u5ea6\uff08~16&#215;10^6cells\/mL\uff09\uff0c\u9ad8\u4e8e\u4f7f\u7528\u4e0d\u8fde\u7eed\u57f9\u517b\u57fa\u7f6e\u6362\u7684\u6447\u74f6\u57f9\u517b\uff0c\u540c\u65f6\u7ef4\u6301VLP\u6ef4\u5ea6\u3002\u6240\u4ee5\uff0cEGE\u662f\u4e00\u79cd\u53ef\u5f3a\u5316\u77ac\u65f6\u8f6c\u67d3\u5de5\u827a\u7684\u975e\u5e38\u6709\u6f5c\u529b\u7684\u7b56\u7565\uff0c\u53ef\u8fdb\u4e00\u6b65\u4f18\u5316\u4ee5\u83b7\u5f97\u66f4\u9ad8\u7684VLP\u6ef4\u5ea6\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">Fontana\u7b49\u4f7f\u7528\u7a33\u5b9a\u7684HEK293\u7ec6\u80de\u7cfb\u751f\u4ea7\u4e86\u72c2\u72ac\u75c5\u6bd2\u6837\u9897\u7c92\u3002\u4f5c\u8005\u4f7f\u75285L\u751f\u7269\u53cd\u5e94\u5668\uff0c\u4ee5\u704c\u6d41\u6a21\u5f0f\uff0c\u57f9\u517b\u7ec6\u80de20\u5929\uff0c\u4f7f\u7528\u65cb\u8f6c\u6ee4\u5668\u622a\u7559\u7ec6\u80de\u3002\u4f7f\u7528\u8fd9\u79cd\u65b9\u6cd5\uff0c\u57f9\u517b\u5bc6\u5ea6\u53ef\u8fbe\u523016&#215;10^6cells\/mL\uff0c\u76f8\u6bd4\u6279\u6b21\u6a21\u5f0f\uff0c\u7ec6\u80de\u5bc6\u5ea6\u7ffb\u500d\u3002\u7531\u4e8e\u57fa\u4e8eVLP\u7684\u75ab\u82d7\u53ef\u7a33\u5b9a\u751f\u4ea7\uff0c\u751f\u7269\u53cd\u5e94\u5668\u5185\u7ec6\u80de\u8d8a\u591a\uff0c\u5373\u76f4\u63a5\u610f\u5473\u7740\u53ef\u751f\u4ea7\u66f4\u591a\u7684\u4ea7\u7269\u3002\u8fd9\u53ef\u964d\u4f4e\u751f\u4ea7\u5de5\u827a\u7684\u6210\u672c\uff0c\u8fdb\u800c\u663e\u8457\u5f71\u54cd\u52a8\u7269\u75ab\u82d7\u7684\u751f\u4ea7\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;color: rgb(22, 87, 136);box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u901a\u8fc7\u704c\u6d41\u514b\u670d\u7ec6\u80de\u5bc6\u5ea6\u6548\u5e94<\/span><\/strong><\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u7ec6\u80de\u5bc6\u5ea6\u6548\u5e94\u662f\u4e00\u79cd\u5e7f\u6cdb\u62a5\u5bfc\u7684\u73b0\u8c61\uff0c\u5373\u7ec6\u80de\u5728\u9ad8\u5bc6\u5ea6\uff080.5-5&#215;10^6cells\/mL\u4ee5\u4e0a\uff0c\u53d6\u51b3\u4e8e\u6bcf\u79cd\u7279\u5b9a\u7684\u7ec6\u80de\u7cfb\uff09\u6761\u4ef6\u4e0b\u611f\u67d3\u6216\u8f6c\u67d3\u65f6\uff0c\u7ec6\u80de\u7279\u5f02\u6027\u4ea7\u7387\u4f1a\u663e\u8457\u964d\u4f4e\u3002\u9020\u6210\u8fd9\u79cd\u73b0\u8c61\u7684\u4e3b\u8981\u5047\u8bbe\u662f\u5728\u9ad8\u5bc6\u5ea6\u6761\u4ef6\u4e0b\uff0c\u8425\u517b\u7269\u8d28\u88ab\u201c\u5265\u593a\u201d\uff0c\u5728\u6b64\u7c7b\u6761\u4ef6\u4e0b\uff0c\u7ec6\u80de\u5f88\u96be\u751f\u4ea7\u9ad8\u75c5\u6bd2\u6ef4\u5ea6\u3002<\/span><span style=\"max-width: 100%;font-size: 14px;color: rgb(255, 255, 255);background-color: rgb(136, 136, 136);box-sizing: border-box !important;word-wrap: break-word !important;\">\u704c\u6d41\u5df2\u88ab\u8bc1\u5b9e\u6709\u52a9\u4e8e\u5ba2\u670d\u201c\u7ec6\u80de\u5bc6\u5ea6\u6548\u5e94\u201d\u7684\u9650\u5236\uff1a\u4ee5\u704c\u6d41\u6a21\u5f0f\u57f9\u517b\u7684\u7ec6\u80de\u5728\u66f4\u9ad8\u7684\u7ec6\u80de\u5bc6\u5ea6\u6761\u4ef6\u4e0b\u88ab\u611f\u67d3\u6216\u8f6c\u67d3\uff0c\u53ef\u7ef4\u6301\u7ec6\u80de\u7279\u5f02\u6027\u4ea7\u7387\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">Genzel\u7b49\u901a\u8fc7\u611f\u67d3AGE\u548cCAP\u7ec6\u80de\u751f\u4ea7\u6d41\u611f\u75c5\u6bd2\uff0c\u4ee5ATF\u7cfb\u7edf\u8fdb\u884c\u704c\u6d41\u3002AGE\u7ec6\u80de\u572850&#215;10^6cells\/mL\u6761\u4ef6\u4e0b\u611f\u67d3\uff0cCAP\u7ec6\u80de\u572825&#215;10^6cells\/mL\u6761\u4ef6\u4e0b\u611f\u67d3\uff0c\u76f8\u6bd4\u4f4e\u7ec6\u80de\u5bc6\u5ea6\u611f\u67d3\uff0c\u4e24\u79cd\u60c5\u51b5\u5747\u53ef\u7ef4\u6301\u7279\u5f02\u6027\u75c5\u6bd2\u4ea7\u7387\uff0c\u660e\u663e\u514b\u670d\u4e86\u7ec6\u80de\u5bc6\u5ea6\u6548\u5e94\u3002Petiot\u7b49\u62a5\u5bfc\u611f\u67d3\u4f7f\u7528\u58f0\u5b66\u8fc7\u6ee4\u5668\u704c\u6d41\u57f9\u517b\u7684HEK293\u7ec6\u80de\uff0c\u751f\u4ea7\u6d41\u611f\u75c5\u6bd2\u3002HEK293\u7ec6\u80de\u5728\u704c\u6d41\u4e2d\u5448\u73b0\u4e2d\u66f4\u52a0\u6d3b\u8dc3\u7684\u4ee3\u8c22\u72b6\u6001\uff0c\u4ece\u800c\u4f7f\u6ef4\u5ea6\u589e\u52a010\u500d\u3002Venereo-Sanchez\u7b49\u4f7f\u7528\u751f\u4ea7\u8840\u51dd\u7d20\uff08HA\uff09\u548c\u795e\u7ecf\u6c28\u9178\u9176\uff08NA\uff09\u7684\u53ef\u8bf1\u5bfc\u7ec6\u80de\u7cfb\u751f\u4ea7\u57fa\u4e8eVLP\u7684\u6d41\u611f\u75ab\u82d7\uff0c\u77ac\u65f6\u8f6c\u67d3Gag-GFP\u4f5c\u4e3aVLP\u7684\u6838\u86cb\u767d\u3002\u901a\u8fc7\u704c\u6d41\u57f9\u517bHEK293\u7ec6\u80de\uff0c\u4ee5\u5728\u8bf1\u5bfcHA\u548cNA\u8868\u8fbe\u53caGag\u8f6c\u67d3\u524d\uff0c\u8fbe\u5230\u9ad8\u7ec6\u80de\u5bc6\u5ea6\uff0814&#215;10^6cells\/mL\uff09\u3002\u7531\u4e8e\u7279\u5f02\u6027\u75c5\u6bd2\u4ea7\u91cf\u4e0d\u53d7\u5f71\u54cd\uff0c\u76f8\u6bd4\u6279\u6b21\u57f9\u517b\uff0c\u704c\u6d41\u57f9\u517b\u7684Gag-GFP\u548cHA\u6ef4\u5ea6\u5206\u522b\u589e\u52a0\u4e8660\u500d\u548c17\u500d\u3002<\/span><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;color: rgb(22, 87, 136);box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u75c5\u6bd2\u6027\u75ab\u82d7\u7684\u8fde\u7eed\u6536\u83b7<\/span><\/strong><\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word 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0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u704c\u6d41\u4e5f\u88ab\u7528\u4e8e\u9006\u8f6c\u5f55\u75c5\u6bd2\u7684\u751f\u4ea7\uff0c\u4f7f\u7528\u60ac\u6d6e\u9a6f\u5316\u7684293GPG\u5305\u88c5\u7ec6\u80de\u3002\u4f7f\u75283L\u751f\u7269\u53cd\u5e94\u5668\u704c\u6d41\u57f9\u517b\uff0c\u7ed3\u679c\u663e\u793a\uff0c\u76f8\u6bd4\u6279\u6b21\u6a21\u5f0f\uff0c\u83ab\u6d1b\u5c3c\u5c0f\u9f20\u767d\u8840\u75c5\u75c5\u6bd2\uff08MoMLV\uff09\u75c5\u6bd2\u9897\u7c92\u6ef4\u5ea6\u589e\u52a02\u500d\uff082.3&#215;10^7IVP\/mL\uff09\u3002Grieger\u7b49\u6d4b\u8bd5\u4e86\u4ece\u60ac\u6d6e\u57f9\u517b\u57fa\u4e2d\u8fde\u7eed\u6536\u83b7rAAV\u7684\u65b9\u6cd5\uff0c\u76f8\u6bd4\u6279\u6b21\u5bf9\u7167\u7ec4\uff0cAAV8\u548cAAV9\u5206\u522b\u589e\u52a0\u4e866.5\u500d\u548c4.8\u500d\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 14px;color: rgb(22, 87, 136);box-sizing: border-box !important;word-wrap: break-word !important;\">\u817a\u75c5\u6bd2\u8f7d\u4f53<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u817a\u75c5\u6bd2\u8f7d\u4f53\u751f\u4ea7\u65f6\uff0c\u5982\u4ee5\u6279\u6b21\u6a21\u5f0f\u8fdb\u884c\uff0c\u5728\u9ad8\u7ec6\u80de\u6d53\u5ea6\u6761\u4ef6\u4e0b\uff0c\u4f1a\u89c2\u5bdf\u5230\u7ec6\u80de\u5bc6\u5ea6\u6548\u5e94\u3002\u5df2\u7ecf\u7814\u7a76\u8fc7\u4e0d\u540c\u7684\u65b9\u6cd5\uff0c\u4ee5\u89c4\u907f\u8fd9\u4e2a\u95ee\u9898\uff0c\u4f8b\u5982\u5728\u8f6c\u67d3\u524d\u540e\u7f6e\u6362\u57f9\u517b\u57fa\u4ee5\u53ca\u901a\u8fc7\u8865\u6599\u5206\u6279\u4f18\u5316\u6279\u6b21\u7ed3\u679c\u3002\u4f46\u704c\u6d41\u662f\u514b\u670d\u817a\u75c5\u6bd2\u751f\u4ea7\u4e2d\u7ec6\u80de\u5bc6\u5ea6\u6548\u5e94\u7684\u6700\u53ef\u884c\u7684\u7b56\u7565\u3002\u5728\u7528\u4e8e\u91cd\u7ec4\u817a\u75c5\u6bd2\u7684293S\u7ec6\u80de\u57f9\u517b\u4e2d\uff0c\u4e3a\u63d0\u9ad8\u7ec6\u80de\u5bc6\u5ea6\uff0c\u4f7f\u7528TFF\u8bbe\u5907\uff0c\u4ee5\u704c\u6d41\u6a21\u5f0f\u8fdb\u884c\u4e86\u817a\u75c5\u6bd2\u7684\u751f\u4ea7\u3002\u57281VVD\u7684\u704c\u6d41\u901f\u7387\u6761\u4ef6\u4e0b\uff0c10\u5929\u5185\uff0c293S\u7ec6\u80de\u751f\u957f\u81f314&#215;10^6cells\/mL\u3002\u7528\u4e8e\u817a\u75c5\u6bd2\u751f\u4ea7\u65f6\uff0c\u4e09\u4e2a293\u7ec6\u80de\u57f9\u517b\u4ee51VVD\u7684\u704c\u6d41\u901f\u7387\uff0c\u5e73\u884c\u8fdb\u884c\uff0c\u5728\u5e73\u5747\u5bc6\u5ea68&#215;10^6cells\/mL\u65f6\u611f\u67d3\uff0c\u5373\u76f8\u5f53\u4e8e\u5bf9\u6570\u751f\u957f\u4e2d\u671f\u3002\u4e00\u4e2a\u57f9\u517b\u8bbe\u7f6e\u4e3a37\u2103\uff0c\u53e6\u4e24\u4e2a\u8bbe\u7f6e\u4e3a35\u2103\u3002\u7ed3\u679c\u662f\uff0c\u572837\u2103\u6761\u4ef6\u4e0b\uff0c\u53ef\u751f\u4ea73.2&#215;10^9\u611f\u67d3\u6027\u75c5\u6bd2\u9897\u7c92\uff08IVP\uff09\/mL\uff0c\u572835\u2103\u6761\u4ef6\u4e0b\u4e3a7.8&#215;10^9IVP\/mL\uff0c\u81f3\u5c11\u662f\u6279\u6b21\u5bf9\u7167\u7ec4\u76845.5\u500d\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">ATF\u704c\u6d41\u8bbe\u5907\u4e5f\u88ab\u6d4b\u8bd5\u7528\u4e8e\u6eb6\u7624\u6027\u817a\u75c5\u6bd2\u8f7d\u4f53\uff08ONYX-411\uff09\u7684\u751f\u4ea7\uff0c\u4f7f\u7528HeLaS3\u7ec6\u80de\u7cfb\u3002\u751f\u7269\u53cd\u5e94\u5668\u4ee5\u6279\u6b21\u6a21\u5f0f\u5f00\u59cb\uff0c\u5f53\u4e73\u9178\u6d53\u5ea6\u8d85\u8fc71.3g\/L\u65f6\uff0c\u5f00\u59cb\u704c\u6d41\u3002\u8865\u6db2\u7b56\u7565\u57fa\u4e8e\u7ef4\u6301\u8461\u8404\u7cd6\u6d53\u5ea6\u9ad8\u4e8e2g\/L\uff0c\u4e14\u4e73\u9178\u6d53\u5ea6\u4f4e\u4e8e2g\/L\u3002\u6bcf\u5929\u4e24\u6b21\u68c0\u67e5\u704c\u6d41\u901f\u7387\uff0c\u4ee5\u6ee1\u8db3\u7ec6\u80de\u57f9\u517b\u8981\u6c42\u3002\u57f9\u517b\u611f\u67d3\u540e\uff0c\u505c\u6b62\u704c\u6d413\u5c0f\u65f6\uff0c\u4ee5\u907f\u514d\u201c\u6d17\u6389\u201d\u7528\u4e8e\u611f\u67d3\u7684\u75c5\u6bd2\u3002\u7ed3\u679c\u663e\u793a\uff0c\u8865\u6599\u5206\u6279\u548c\u704c\u6d41\u57f9\u517b\u7684\u5e73\u5747\u7ec6\u80de\u7279\u5f02\u6027\u75c5\u6bd2\u4ea7\u7387\u76f8\u5f53\uff0c\u5206\u522b\u4e3a3.1&#215;10^4VP\/cell\u548c3.3&#215;10^4VP\/cell\u3002\u4f46\u662f\u6536\u83b7\u65f6\uff0c\u704c\u6d41\u57f9\u517b\u4e2d\u68c0\u6d4b\u5230\u7684\u5e73\u5747\u75c5\u6bd2\u6ef4\u5ea6\u7ea6\u4e3a\u8865\u6599\u5206\u6279\u57f9\u517b\u76844\u500d\uff0c\u56e0\u4e3a\u5728\u611f\u67d3\u65f6\uff0c\u6d3b\u7ec6\u80de\u5bc6\u5ea6\u5dee\u4e3a4\u500d\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;color: rgb(22, 87, 136);box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u603b\u7ed3\u548c\u5c55\u671b<\/span><\/strong><\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word 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style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u751f\u7269\u5236\u836f\u884c\u4e1a\u7684\u751f\u4ea7\u6a21\u5f0f\u7684\u8f6c\u53d8\u6b63\u5728\u53d1\u751f\uff0c\u6279\u6b21\u548c\u8865\u6599\u5206\u6279\u6a21\u5f0f\u6b63\u9010\u6e10\u88ab\u8fde\u7eed\u751f\u4ea7\u53d6\u4ee3\u3002\u4f7f\u7528\u8fde\u7eed\u6280\u672f\u83b7\u5f97\u7684\u75c5\u6bd2\u9897\u7c92\u751f\u4ea7\u7684\u6210\u529f\u6848\u4f8b\u3001\u65b0\u7684\u76d1\u6d4b\u548c\u63a7\u5236\u6280\u672f\u7684\u53d1\u5c55\u4ee5\u53ca\u8fde\u7eed\u4e0b\u6e38\u5de5\u827a\u7684\u6574\u5408\uff0c\u5c06\u6781\u5927\u7684\u4fc3\u8fdb\u8fde\u7eed\u5e73\u53f0\u7684\u5de9\u56fa\uff0c\u4ee5\u7528\u4e8e\u4f5c\u4e3a\u6cbb\u7597\u6027\u836f\u7269\u7684\u75c5\u6bd2\u7684\u751f\u4ea7\u548c\u7eaf\u5316\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><em style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 12px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;color: rgb(22, 87, 136);box-sizing: border-box !important;word-wrap: break-word !important;\">\u25a3<\/span>&nbsp;<span style=\"max-width: 100%;color: rgb(22, 87, 136);box-sizing: border-box !important;word-wrap: break-word !important;\">\u25a3<\/span><\/span><\/em><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><em style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 12px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u672c\u6587\u8282\u9009\u81ea\u4ee5\u4e0b\u6587\u7ae0\uff0c\u56e0\u6c34\u5e73\u6709\u9650\uff0c\u5982\u6709\u4e0d\u5f53\u4e4b\u5904\uff0c\u656c\u8bf7\u8c05\u89e3\u3002\u8be6\u7ec6\u7684\u5b8c\u6574\u5185\u5bb9\uff0c\u8bf7\u53c2\u8003\u539f\u6587\u3002<\/span><\/em><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><em style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 12px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u53c2\u8003\u539f\u6587\uff1a<\/span><\/em><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;white-space: normal;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;\"><em style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 12px;box-sizing: border-box !important;word-wrap: break-word !important;\">S.Gutierrez-Granados, F.Godia, L.Cervera, Continuous manufacturing of viral particles. Current Opinion In Chemical Engineering. 2018, 22: 107-114.&nbsp;<\/span><\/em><\/p>\n<p style=\"white-space: normal;max-width: 100%;min-height: 1em;letter-spacing: 0.544px;font-size: 16px;background-color: rgb(255, 255, 255);font-family: \u5fae\u8f6f\u96c5\u9ed1;line-height: 32px;text-align: center;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box;text-indent: 27px;letter-spacing: 0.54px;widows: 1;font-size: 18px;overflow-wrap: break-word !important;\"><span style=\"max-width: 100%;box-sizing: border-box;color: rgb(0, 209, 0);overflow-wrap: break-word !important;\">\u4f1a\u8bae\u63a8\u8350<\/span><\/strong><\/p>\n<p style=\"white-space: normal;max-width: 100%;min-height: 1em;letter-spacing: 0.544px;font-size: 16px;background-color: rgb(255, 255, 255);font-family: \u5fae\u8f6f\u96c5\u9ed1;line-height: 32px;text-align: center;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><span style=\"max-width: 100%;box-sizing: border-box;color: rgb(0, 209, 0);overflow-wrap: break-word !important;\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzA5MDY4MDQ1NQ==&amp;mid=2649833891&amp;idx=1&amp;sn=d9bb5b15968ccaefb4dd637743b1df69&amp;chksm=88021c4bbf75955d4648aa267b7db72e894ebc9f5748ee27745b19051591a65203c1c5a274e9&amp;scene=21#wechat_redirect\" target=\"_blank\"  style=\"color: rgb(87, 107, 149);-webkit-tap-highlight-color: rgba(0, 0, 0, 0);max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\">\u5927\u5496\u9f50\u805a\u4e0a\u6d77|CAR-T\u7ec6\u80de\u6cbb\u7597\u7814\u53d1\u53ca\u751f\u4ea7\u5de5\u827a\u8bba\u575b<\/a><\/span><\/strong><\/p>\n<p style=\"font-variant-numeric: normal;font-variant-east-asian: normal;white-space: normal;max-width: 677px;box-sizing: border-box;min-height: 16px;font-size: 16px;background-color: rgb(255, 255, 255);font-family: \u5fae\u8f6f\u96c5\u9ed1;letter-spacing: 0.54px;line-height: 27.2px;text-align: center;widows: 1;overflow-wrap: break-word !important;\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzA5MDY4MDQ1NQ==&amp;mid=2649833899&amp;idx=2&amp;sn=0d99c5bd6ab2e51d93d8d7b1234ed00d&amp;chksm=88021c43bf7595551d94b8eabdf1ba2dfcbbfe23b1a3ee4876d77cf7c69604dfd71d18730978&amp;scene=21#wechat_redirect\" target=\"_blank\"  style=\"color: rgb(87, 107, 149);text-decoration: underline;-webkit-tap-highlight-color: rgba(0, 0, 0, 0);max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\">\u5927\u5496\u9f50\u805a\u5317\u4eac|\u56fd\u9645\u751f\u7269\u6cbb\u7597\u4ea7\u4e1a\u5927\u4f1a<\/strong><\/a><\/p>\n<p style=\"font-variant-numeric: normal;font-variant-east-asian: normal;white-space: normal;max-width: 677px;box-sizing: border-box;min-height: 16px;font-size: 16px;background-color: rgb(255, 255, 255);font-family: \u5fae\u8f6f\u96c5\u9ed1;letter-spacing: 0.54px;line-height: 27.2px;text-align: center;widows: 1;overflow-wrap: break-word !important;\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MjM5NTI3MDE0NQ==&amp;mid=2653621905&amp;idx=5&amp;sn=8fb8c002bc9fa9fc4323d951e10707c0&amp;chksm=bd24b5ff8a533ce9b71923a658e4a0051fe456ab01bb04ba897948ee75b399b003197b31bb05&amp;scene=21#wechat_redirect\" target=\"_blank\"   style=\"color: rgb(87, 107, 149);text-decoration: underline;-webkit-tap-highlight-color: rgba(0, 0, 0, 0);max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\">2019\u7b2c\u4e09\u5c4a\u56fd\u9645\u751f\u7269\u6cbb\u7597\u5927\u4f1a\u66a8\u5c55\u89c8\u4f1a<\/strong><\/a><br style=\"max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\"  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