{"id":103922,"date":"2019-02-22T00:00:50","date_gmt":"2019-05-17T08:54:16","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/103922\/"},"modified":"2019-05-17T16:54:18","modified_gmt":"2019-05-17T08:54:18","slug":"c612a531cb","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/103922\/","title":{"rendered":"\u566c\u83cc\u4f53\u5c55\u793a\u539f\u7406\u548c\u5b9e\u8df5\uff1a\u6784\u67b6\u7bc7\uff08\u4e0b\uff09"},"content":{"rendered":"<div class=\"bpp-post-content\">\n<section  label=\"Powered by 135editor.com\" style=\"font-size:16px;\">\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"text-align: center;margin: 11px 0% 10px;box-sizing: border-box;\">\n<section style=\"display: inline-block;width: 100%;vertical-align: top;border-color: #6ccbef;border-width: 2px;border-radius: 0px;border-style: solid;padding-right: 10px;padding-bottom: 10px;padding-left: 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SimSun;\">\u6dd8\u9009\u548c\u7b5b\u9009\u7684\u8fc7\u7a0b\u4e2d\u5747\u6709\u566c\u83cc\u4f53\u548c\u9776\u70b9\u6297\u539f\u7684\u76f8\u4e92\u4f5c\u7528\u6b65\u9aa4\uff0c\u5982\u4f55\u6839\u636e\u5b9e\u9a8c\u7ed3\u679c\u8c03\u6574\u566c\u83cc\u4f53\u548c\u9776\u70b9\u6297\u539f\u7684\u76f8\u5bf9\u591a\u5c11\u662f\u6dd8\u9009\u65b9\u6cd5\u548c\u7b5b\u9009\u65b9\u6cd5\u4f18\u5316\u7684\u5173\u952e\u3002\u566c\u83cc\u4f53\u548c\u9776\u70b9\u6297\u539f\u76f8\u4e92\u4f5c\u7528\u7684\u5b9e\u8d28\u662f\u86cb\u767d\u95f4\u7684\u53ef\u9006\u53cd\u5e94\uff0c\u53ef\u9006\u53cd\u5e94\u7684\u8fc7\u7a0b\u610f\u5473\u7740:\u6709\u591a\u5c11\u7684\u9633\u6027\u566c\u83cc\u4f53\u80fd\u591f\u548c\u6297\u539f\u7ed3\u5408\uff0c\u9664\u4e86\u548c\u4e24\u8005Input\u7684\u91cf\u76f8\u5173\u4ee5\u5916\u8fd8\u548c\u4e24\u8005\u4e4b\u95f4\u7684\u4eb2\u548c\u529b\u5927\u5c0f(\u89e3\u79bb\u5e73\u8861\u5e38\u6570Kd)\u76f8\u5173\uff0cJohnMcCafferty\u5728PhageDisplay of Peptides and Proteins A Laboratory Manual\u4e00\u4e66\u7684\u7b2c\u4e03\u7ae0\u8282PhageDisplay: Factors Affecting PanningEfficiency\u4e2d\u6709\u8fc7\u5b9e\u9a8c\u548c\u7406\u8bba\u7684\u63a8\u5bfc<sup>2<\/sup>\uff0c\u7b80\u800c\u6982\u4e4b\u53ef\u4ee5\u7528\u4e0b\u9762\u7684\u516c\u5f0f\u8fdb\u884c\u63cf\u8ff0\uff1a<\/span><\/p>\n<p style=\"margin-bottom: 0in;\"><img loading=\"lazy\" align=\"bottom\" border=\"0\" class=\"\"     height=\"88\" vspace=\"1\" width=\"469\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/8-1558083256.png\" alt=\"\u566c\u83cc\u4f53\u5c55\u793a\u539f\u7406\u548c\u5b9e\u8df5\uff1a\u6784\u67b6\u7bc7\uff08\u4e0b\uff09\" title=\"\u566c\u83cc\u4f53\u5c55\u793a\u539f\u7406\u548c\u5b9e\u8df5\uff1a\u6784\u67b6\u7bc7\uff08\u4e0b\uff09\"  \/><\/p>\n<p style=\"text-indent: 0.2in;margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">\u8be5\u516c\u5f0f\u8bf4\u660e\u4e86\u6dd8\u9009\u548c\u7b5b\u9009\u8fc7\u7a0b\u4e2d\u6297\u539f\u7684\u6d53\u5ea6\u9009\u62e9\u5bf9\u4e8e\u6dd8\u9009\u6548\u7387\u548c\u7b5b\u9009\u533a\u5206\u5ea6\u7684\u5f71\u54cd\u3002<\/span><\/p>\n<p style=\"margin-bottom: 0.11in;text-indent: 2em;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">\u5bf9\u4e8e\u8be5\u516c\u5f0f\u7684\u4f7f\u7528\u7b14\u8005\u9009\u53d6\u4eb2\u548c\u529bKd\u4e3a1nM\u7684\u6297\u4f53Ab1\u548c\u9009\u53d6\u4eb2\u548c\u529bKd\u4e3a10nM\u7684\u6297\u4f53Ab2\u4e3a\u4f8b\u4ee5\u4e0b\u56fe\u8fdb\u884c\u8bf4\u660e\u3002\u5982\u679cinput\u7684\u5c55\u793aAb1\uff08\u7ea2\u8272\u5b9e\u7ebf\uff09\u548c\u5c55\u793aAb2\uff08\u84dd\u8272\u5b9e\u7ebf\uff09\u7684\u566c\u83cc\u4f53\u7684\u91cf\u662f\u4e00\u6837\u7684\u3002<\/span><\/p>\n<p style=\"margin-bottom: 0.11in;text-indent: 2em;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">\u5728\u6297\u539f\u6d53\u5ea6\u4e3a10nM\u7684\u6761\u4ef6\u4e0b\uff0c90%\u7684Ab1\u5c55\u793aphage\u548c50%\u7684Ab2\u5c55\u793aphage\u80fd\u591f\u88ab\u6297\u539f\u6355\u83b7\uff0c\u8fd9\u6837\u4eb2\u548c\u529b\u5f3a\u7684Ab1\u76f8\u5bf9\u4e8e\u4eb2\u548c\u529b\u5f31\u7684Ab2\u6709\u66f4\u591a\u7684phage\u88ab\u6dd8\u9009\u4e0b\u6765\u8fdb\u5165\u4e0b\u4e00\u8f6e\u7684\u6269\u589e\u548c\u6dd8\u9009\uff0c\u5982\u6b642-3\u8f6e\uff0c\u5f3a\u4eb2\u548c\u529b\u7684Ab1\u5c55\u793a\u566c\u83cc\u4f53\u76f8\u5bf9\u4e8e\u5f31\u4eb2\u548c\u529b\u7684Ab2\u5c55\u793a\u566c\u83cc\u4f53\u6709\u66f4\u591a\u7684\u5bcc\u96c6\uff0c\u5f31\u4eb2\u548c\u529b\u7684Ab2\u76f8\u5bf9\u4e8e\u975e\u7279\u5f02\u6216\u66f4\u5f31\u7ed3\u5408\u7684phage\u6709\u66f4\u591a\u7684\u5bcc\u96c6\uff1b<\/span><\/p>\n<p style=\"margin-bottom: 0.11in;\"><img loading=\"lazy\" align=\"left\" class=\"\"     height=\"241\" hspace=\"12\" width=\"461\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/4-1558083256.png\" alt=\"\u566c\u83cc\u4f53\u5c55\u793a\u539f\u7406\u548c\u5b9e\u8df5\uff1a\u6784\u67b6\u7bc7\uff08\u4e0b\uff09\" title=\"\u566c\u83cc\u4f53\u5c55\u793a\u539f\u7406\u548c\u5b9e\u8df5\uff1a\u6784\u67b6\u7bc7\uff08\u4e0b\uff09\"  \/><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\"> &nbsp;<br clear=\"left\"  \/> &nbsp; \u5982\u679c\u5c06\u6297\u539f\u6d53\u5ea6\u4e3a1nM\u7684\u6761\u4ef6\u4e0b\uff0c50%\u7684Ab1\u5c55\u793aphage\u548c9.1%\u7684Ab2\u5c55\u793aphage\u80fd\u591f\u88ab\u6297\u539f\u6355\u83b7\uff0c\u65e0\u8bbaAb1\u8fd8\u662fAb2\u6dd8\u9009\u7684phage\u5747\u6709\u51cf\u5c11\uff0c\u4f46\u662f\u6dd8\u9009\u4e0b\u6765\u7684Ab1\/Ab2\u7684\u91cf\u6bd4\u503c\u7531\u6297\u539f\u6d53\u5ea6\u4e3a10nM\u65f61.8\u500d\u4e0a\u5347\u5230\u4e865.5\u500d(\u7eff\u8272\u865a\u7ebf)\uff0c\u6297\u539f\u6d53\u5ea6\u51cf\u5c11\u65f6\uff0cAb1\u76f8\u5bf9\u4e8eAb2\u6709\u66f4\u591a\u7684\u5bcc\u96c6\uff0c\u6297\u539f\u7684\u6d53\u5ea6\u8d8a\u5c11\u5bf9\u6297\u4f53\u7684\u4eb2\u548c\u529b\u5927\u5c0f\u8d8a\u5177\u6709\u9009\u62e9\u6027\u3002<\/span><\/p>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\"  >\n<section class=\"135brush\"   style=\"color: rgb(5, 115, 175);padding: 0.45em 0.6em 0.45em 0.9em;border-left: 10px solid rgb(83, 191, 250);border-top-color: rgb(83, 191, 250);border-right-color: rgb(83, 191, 250);border-bottom-color: rgb(83, 191, 250);background: rgb(182, 228, 253);box-sizing: border-box;\">\u6dd8\u9009\u548c\u7b5b\u9009\u4f18\u5316\u7684\u57fa\u672c\u539f\u5219<\/section>\n<\/section>\n<\/section>\n<p style=\"margin-bottom: 0.11in;text-indent: 2em;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">\u6297\u539f\u6d53\u5ea6\u7684\u53d8\u5316\u5f15\u8d77\u7684\u4e0d\u540c\u4eb2\u548c\u529b\u7684\u9633\u6027\u566c\u83cc\u4f53\u7ed3\u5408\u7684\u91cf\u548c\u6bd4\u4f8b\u7684\u53d8\u5316\u89c4\u5f8b\u6307\u5bfc\u5b9e\u9a8c\u4eba\u5458\u5728\u6dd8\u9009\u8fc7\u7a0b\u4e2d\u6297\u539f\u6d53\u5ea6\u6761\u4ef6\u7684\u8bbe\u5b9a\u65f6\uff0c\u4ece\u7b2c\u4e00\u8f6e\u6dd8\u9009\u5230\u7b2c\u4e09\u8f6e\u6dd8\u9009\uff0c\u6297\u539f\u6d53\u5ea6\u5e94\u662f\u9010\u6b65\u964d\u4f4e\u7684\u8fc7\u7a0b\u3002<sup>3<\/sup><\/span><\/p>\n<p style=\"margin-bottom: 0.11in;text-indent: 2em;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">\u7b2c\u4e00\u8f6e\u6dd8\u9009\u8fc7\u7a0b\u4e2dinput\u7684\u566c\u83cc\u4f53\u6587\u5e93\u4e2d\u591a\u6837\u6027\u7684\u6bcf\u4e2a\u566c\u83cc\u4f53\u7684\u62f7\u8d1d\u6570\u4e00\u822c\u4e3a10-100\u4e2a\uff0c\u4e3a\u4e86\u5c3d\u53ef\u80fd\u5c06\u5f31\u7ed3\u5408\u80fd\u529b(uM\u7ea7\u522b)\u7684\u6297\u4f53\u5c55\u793aphage\u4e5f\u4fdd\u7559\u4f4f\uff0c\u9ad8\u6d53\u5ea6\u7684\u6297\u539f\u598210ug\/ml\u8fdb\u884c\u6dd8\u9009\u662f\u975e\u5e38\u6709\u52a9\u4e8e\u5f97\u5230\u4e00\u4e2a\u4eb2\u548c\u529b\u8de8\u5ea6\u8f83\u5927\u591a\u6837\u6027\u8f83\u597d\u7684\u9633\u6027\u566c\u83cc\u4f53\u6587\u5e93\u3002<\/span><\/p>\n<p><img loading=\"lazy\" align=\"left\" class=\"\"     height=\"244\" hspace=\"13\" width=\"516\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/3-1558083256.png\" alt=\"\u566c\u83cc\u4f53\u5c55\u793a\u539f\u7406\u548c\u5b9e\u8df5\uff1a\u6784\u67b6\u7bc7\uff08\u4e0b\uff09\" title=\"\u566c\u83cc\u4f53\u5c55\u793a\u539f\u7406\u548c\u5b9e\u8df5\uff1a\u6784\u67b6\u7bc7\uff08\u4e0b\uff09\"  \/><\/p>\n<p style=\"margin-bottom: 0.11in;text-indent: 0em;\"><br  \/><\/p>\n<p style=\"margin-bottom: 0.11in;text-indent: 2em;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, 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style=\"margin-bottom: 0.11in;\"><img loading=\"lazy\" align=\"left\" class=\"\"     height=\"248\" hspace=\"13\" width=\"504\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/6-1558083257.png\" alt=\"\u566c\u83cc\u4f53\u5c55\u793a\u539f\u7406\u548c\u5b9e\u8df5\uff1a\u6784\u67b6\u7bc7\uff08\u4e0b\uff09\" title=\"\u566c\u83cc\u4f53\u5c55\u793a\u539f\u7406\u548c\u5b9e\u8df5\uff1a\u6784\u67b6\u7bc7\uff08\u4e0b\uff09\"  \/><\/p>\n<p style=\"text-indent: 0.39in;margin-bottom: 0.11in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">\u7ecf<\/span><span style=\"font-family: \u5b8b\u4f53, SimSun;font-size: 15px;text-indent: 0.39in;caret-color: red;\">\u8fc7\u5408\u9002\u8f6e\u6570\u7b5b\u9009\u9633\u6027\u566c\u83cc\u4f53\u5f97\u4ee5\u5bcc\u96c6\u5e76\u4e14\u9633\u6027\u591a\u6837\u6027\u5f97\u4ee5\u4fdd\u7559\u7684\u9633\u6027\u566c\u83cc\u4f53\u5e93\u53ef\u611f\u67d3TG1\u540e\u6311\u9009\u5355\u514b\u9686\u540e\u8fdb\u884c\u5229\u7528ELISA\u8fdb\u884c\u9ad8\u901a\u91cf\u7b5b\u9009,\u9ad8\u901a\u91cf\u7b5b\u9009\u7684\u76ee\u7684\u662f\u5c06\u4e0d\u540c\u9633\u6027\u566c\u83cc\u4f53\u7684\u4eb2\u548c\u529b\u5f3a\u5f31\u5f97\u4ee5\u533a\u5206,ELISA\u68c0\u6d4b\u4fe1\u53f7\u5f3a\u7684\u514b\u9686\u5176\u4eb2\u548c\u529b\u5f3a.\u8981\u8fbe\u5230\u4ee5\u4e0a\u7684\u76ee\u7684,\u6839\u636e\u4e0a\u6587\u7684\u516c\u5f0f\u4e24\u4e2a\u4eb2\u548c\u529b\u76f8\u5dee10\u500d\u7684\u5c55\u793a\u566c\u83cc\u4f53\u5728\u6297\u539f\u6d53\u5ea6\u4e3a10nM,\u5176\u4fe1\u53f7\u5dee\u5f02\u4e3a1.8\u500d,\u800c\u5728\u6297\u539f\u6d53\u5ea6\u4e3a1nM\u65f6\u5176\u4fe1\u53f7\u5dee\u5f02\u4e3a5.5\u500d,\u5c3d\u53ef\u80fd\u5c11\u7684\u6297\u539f\u6d53\u5ea6\u548c\u9633\u6027\u566c\u83cc\u4f53\u8fdb\u884c\u53cd\u5e94\u662f\u63d0\u9ad8\u7b5b\u9009\u4fe1\u53f7\u5927\u5c0f\u548c\u4eb2\u548c\u529b\u5f3a\u5f31\u7684\u5173\u952e.\u5728\u5b9e\u9645\u64cd\u4f5c\u8fc7\u7a0b\u4e2d,\u53d7\u9650\u4e8e\u5fae\u5b54\u677f\u4e2d\u566c\u83cc\u4f53\u7684\u4ea7\u91cf,ELISA\u65b9\u6cd5\u7684\u68c0\u6d4b\u7075\u654f\u6027,ELISA\u5404\u4e2a\u73af\u8282\u7684\u975e\u7279\u5f02\u4ea4\u53c9\u53cd\u5e94\u7b49,\u4e00\u4e2a\u533a\u5206\u5ea6\u5f88\u597d\u7684\u566c\u83cc\u4f53\u5c55\u793a\u7b5b\u9009\u65b9\u6cd5\u9700\u8981\u5bf9ELISA\u5404\u4e2a\u73af\u8282\u7684\u5404\u4e2a\u8bd5\u5242\u8017\u6750\u8fdb\u884c\u4f18\u5316,\u4e0b\u56fe\u662f\u7b14\u8005\u8fdb\u884c\u4e00\u4e2a\u566c\u83cc\u4f53\u5c55\u793a\u7b5b\u9009\u65b9\u6cd5\u5f00\u53d1\u65f6\u7684\u4f18\u5316\u65b9\u5411\u4ee5\u4f9b\u5404\u4f4d\u540c\u884c\u53c2\u8003,\u5177\u4f53\u5185\u5bb9\u6682\u4e0d\u4f5c\u5c55\u5f00\u3002<\/span><\/p>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\"  >\n<section class=\"135brush\"   style=\"color: rgb(5, 115, 175);padding: 0.45em 0.6em 0.45em 0.9em;border-left: 10px solid rgb(83, 191, 250);border-top-color: rgb(83, 191, 250);border-right-color: rgb(83, 191, 250);border-bottom-color: rgb(83, 191, 250);background: rgb(182, 228, 253);box-sizing: border-box;\">\u6dd8\u9009\u6548\u7387\u7684\u5176\u4ed6\u8003\u91cf<\/section>\n<\/section>\n<\/section>\n<p style=\"margin-bottom: 0.11in;text-indent: 2em;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">\u566c\u83cc\u4f53\u6dd8\u9009\u7684\u8fc7\u7a0b\u51b3\u5b9a\u4e86\u80fd\u591f\u5f97\u5230\u591a\u5c11\u7279\u5f02\u7684\u9633\u6027\u566c\u83cc\u4f53\uff0c\u7b5b\u9009\u7684\u8fc7\u7a0b\u662f\u4ee5\u6570\u636e\u5927\u5c0f\u7684\u5f62\u5f0f\u5bf9\u591a\u6837\u6027\u7684\u9633\u6027\u566c\u83cc\u4f53\u7684\u4eb2\u548c\u529b\u505a\u4e00\u4e2a\u521d\u6b65\u7684\u6bd4\u8f83\u6392\u5e8f\u3002\u76f8\u8f83\u800c\u8a00\uff0c\u6dd8\u9009\u8fc7\u7a0b\u5728\u6297\u4f53\u53d1\u73b0\u8fc7\u7a0b\u4e2d\u6709\u7740\u66f4\u91cd\u8981\u7684\u5730\u4f4d\uff0c\u5728\u6dd8\u9009\u8fc7\u7a0b\u4e2d\u9664\u4e86\u8003\u8651\u566c\u83cc\u4f53\u548c\u9776\u70b9\u6297\u539f\u7684\u7ed3\u5408\u5bcc\u96c6\u5916\uff0c\u8fd8\u9700\u8981\u8003\u8651\u566c\u83cc\u4f53\u548c\u6dd8\u9009\u8fc7\u7a0b\u4e2d\u5176\u4ed6\u8bd5\u5242\u7269\u8d28\u6750\u6599\u7684\u975e\u7279\u5f02\u6216\u8005\u975e\u9884\u671f\u7684\u7ed3\u5408\uff0c\u5982\u566c\u83cc\u4f53\u6dd8\u9009\u6eb6\u6db2\u4e2d\u7684\u5c01\u95ed\u5242\u86cb\u767d\u6210\u5206\u7684\u7ed3\u5408\u6216\u8005\u566c\u83cc\u4f53\u548c\u56fa\u4f53\u8f7d\u4f53\u95f4\u7684\u975e\u7279\u5f02\u5438\u9644\u7b49\uff0c\u7ecf\u8fc72-3\u8f6e\u6dd8\u9009\u540e\u4e5f\u80fd\u591f\u8fdb\u884c\u5bcc\u96c6\uff0c\u8fd9\u6837\u5982\u4e0b\u56fe\u6240\u793a\u5c06\u566c\u83cc\u4f53\u6587\u5e93\u5728\u548c\u6297\u539f\u76f8\u4e92\u4f5c\u7528\u4e4b\u524d\u6216\u8005\u76f8\u4e92\u4f5c\u7528\u4e4b\u540e\u548c\u9664\u6297\u539f\u5916\u7684\u5176\u4ed6\u8bd5\u5242\u6750\u6599\u6210\u5206\u63d0\u524d\u5b75\u80b2\u4e00\u6bb5\u65f6\u95f4\u8fdb\u884c\u8d1f\u5411\u7b5b\u9009\u662f\u5f88\u6709\u5fc5\u8981\u7684\u4e8b\u60c5<sup>4<\/sup>\u3002\u8d1f\u5411\u7b5b\u9009\u7684\u6a21\u5f0f\u5bf9\u4e8e\u4ee5\u7ec6\u80de\u4e3a\u8f7d\u4f53\u7684\u9776\u70b9(\u80bf\u7624\u539f\u4ee3\u7ec6\u80de\u6216\u8005\u9ad8\u5cf0\u5ea6\u8868\u8fbe\u6297\u539f\u7684\u7a33\u8f6c\u7ec6\u80de)\u6dd8\u9009\uff0c\u86cb\u767d\u9776\u70b9\u7279\u5b9a\u4f4d\u70b9\uff08\u5982pMHC\uff09\u7684\u6dd8\u9009\u5747\u6709\u5f88\u597d\u7684\u6307\u5bfc\u610f\u4e49\u3002<\/span><\/p>\n<p style=\"margin-bottom: 0.11in;\"><img loading=\"lazy\" align=\"bottom\" border=\"0\" class=\"\"     height=\"380\" width=\"509\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/3-1558083257.jpg\" alt=\"\u566c\u83cc\u4f53\u5c55\u793a\u539f\u7406\u548c\u5b9e\u8df5\uff1a\u6784\u67b6\u7bc7\uff08\u4e0b\uff09\" title=\"\u566c\u83cc\u4f53\u5c55\u793a\u539f\u7406\u548c\u5b9e\u8df5\uff1a\u6784\u67b6\u7bc7\uff08\u4e0b\uff09\"  \/><\/p>\n<p style=\"margin-bottom: 0.11in;text-indent: 2em;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, 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class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\"  >\n<section class=\"135brush\"   style=\"color: rgb(5, 115, 175);padding: 0.45em 0.6em 0.45em 0.9em;border-left: 10px solid rgb(83, 191, 250);border-top-color: rgb(83, 191, 250);border-right-color: rgb(83, 191, 250);border-bottom-color: rgb(83, 191, 250);background: rgb(182, 228, 253);box-sizing: border-box;\">\u5c55\u671b\uff1a<\/section>\n<\/section>\n<\/section>\n<p style=\"margin-bottom: 0.11in;text-indent: 2em;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">\u566c\u83cc\u4f53\u5c55\u793a\u76f8\u5bf9\u4e8e\u6742\u4ea4\u7624\u5728\u8fdb\u884c\u6297\u4f53\u53d1\u73b0\u7684\u8fc7\u7a0b\u4e2d\u6709\u4e00\u4e2a\u975e\u5e38\u5927\u7684\u4f18\u52bf\u7684\u4e00\u70b9\u5728\u4e8e\u566c\u83cc\u4f53\u5c55\u793a\u53ef\u6dfb\u52a0\u8d1f\u5411\u7b5b\u9009\u7684\u8fc7\u7a0b\uff0c\u8be5\u8fc7\u7a0b\u80fd\u591f\u4f7f\u566c\u83cc\u4f53\u5c55\u793a\u6280\u672f\u80fd\u591f\u5bf9\u4e8e\u9776\u70b9\u6297\u539f\u7684\u7279\u5b9a\u8868\u4f4d\u8fdb\u884c\u7cbe\u786e\u7684\u6297\u4f53\u53d1\u73b0\uff0c\u76f8\u8f83\u4e8e\u6742\u4ea4\u7624\u6280\u672f\u6709\u66f4\u9ad8\u7684\u6297\u4f53\u53d1\u73b0\u6548\u7387\u5982pMHC\u4e3a\u9776\u70b9\u7684TCRlikeantibody\u7684\u53d1\u73b0<sup>5<\/sup>\u3002\u6297\u539f\u6d53\u5ea6\u5bf9\u566c\u83cc\u4f53\u5c55\u793a\u6dd8\u9009\u7ed3\u679c\u7684\u5f71\u54cd\u53ef\u4ee5\u7406\u89e3\u4e3a\u4e00\u79cd\u6dd8\u9009\u538b\u529b\uff0c\u6297\u539f\u6d53\u5ea6\u9ad8\u5219\u6dd8\u9009\u538b\u529b\u4f4e\uff0c\u6297\u539f\u6d53\u5ea6\u4f4e\u5219\u6dd8\u9009\u538b\u529b\u9ad8\uff0c\u6dd8\u9009\u538b\u529b\u9664\u6297\u539f\u6d53\u5ea6\u5916\uff0c\u6db2\u76f8\u56fa\u76f8\u6dd8\u9009\uff0c\u6e29\u5ea6\uff0cpH\u503c<sup>6<\/sup>\uff0c\u6a21\u62df\u80bf\u7624\u5fae\u73af\u5883\u7684Buffer\u7684\u6210\u5206\u7b49\u90fd\u53ef\u4ee5\u4f5c\u4e3a\u4e00\u79cd\u53ef\u8c03\u8282\u7684\u6dd8\u9009\u538b\u529b\uff0c\u901a\u8fc7\u7075\u6d3b\u7684\u8c03\u8282\u6dd8\u9009\u538b\u529b\u53ef\u4ee5\u4ece\u566c\u83cc\u4f53\u6587\u5e93\u5f97\u5230\u66f4\u591a\u6216\u8005\u4eb2\u548c\u529b\u66f4\u5f3a\u6216\u8005\u6709\u67d0\u79cd\u7279\u6027\u7684\u6297\u4f53\uff0c\u5728\u4e2d\u56fd\u65b0\u53d1\u9010\u6b65\u52a0\u5165\u5168\u7403\u8303\u56f4\u5185\u7684\u7ade\u4e89\u683c\u5c40\u4e0b\uff0c\u6709\u7279\u8272\u7684\u5c06\u66f4\u5177\u5907\u4ef7\u503c\uff0c\u566c\u83cc\u4f53\u5c55\u793a\u6280\u672f\u7684\u591a\u65b9\u9762\u66f4\u7cbe\u5bc6\u5e94\u7528\u5c06\u662f\u6838\u5fc3\u7ade\u4e89\u529b\u7684\u6e90\u5934\u6280\u672f\u3002<\/span><\/p>\n<p style=\"text-indent: 0.39in;margin-bottom: 0.11in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">\u5e0c\u671b\u540c\u884c\u4ece\u4e1a\u8005\u5f7c\u6b64\u4e4b\u95f4\u80fd\u591f\u76f8\u4e92\u4ea4\u6d41\u8ba8\u8bba\u78e8\u783a\u6280\u80fd\uff0c\u6709\u610f\u8005\u53ef\u8054\u7cfbbiocommunity@126.com\u4e00\u8d77\u4ea4\u6d41\u8ba8\u8bba\u6210\u957f\u8fdb\u6b65\uff0c\u4e3a\u4ece\u4e1a\u8005\u63d0\u4f9b\u66f4\u4e3a\u8d34\u8fd1\u5b9e\u8df5\u7684\u6280\u672f\u4fe1\u606f\u3002<\/span><\/p>\n<p style=\"margin-bottom: 0.11in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">\u53c2\u8003\u8d44\u6599\uff1a<\/span><\/p>\n<ol class=\"list-paddingleft-2\" style=\"\">\n<li>\n<p style=\"margin-bottom: 0.11in;\"><span style=\"background: #FFFFFF;color: #000000;font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">Schofield\tD J , Pope A R , Clementel V , et al. Application of phage display\tto high throughput antibody generation and characterization[J].\tGenome biology, 2007, 8(11):R254.<\/span><\/p>\n<\/li>\n<li>\n<p style=\"margin-bottom: 0.11in;\"><span style=\"background: #FFFFFF;color: #000000;font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">McCafferty,\tJohn. Phage display of peptides and proteins a laboratory manual\t\/[M]\/\/ Phage display of peptides and proteins : a laboratory manual.\tAcademic Press, 1996.<\/span><\/p>\n<\/li>\n<li>\n<p style=\"margin-bottom: 0.11in;\"><span style=\"background: #FFFFFF;color: #000000;font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">Baker\tK P , Edwards B M , Main S H , et al. Generation and\tcharacterization of LymphoStat-B, a human monoclonal antibody that\tantagonizes the bioactivities of B lymphocyte stimulator[J].\tArthritis and rheumatism, 2003, 48(11):3253-3265.<\/span><\/p>\n<\/li>\n<li>\n<p style=\"margin-bottom: 0.11in;\"><span style=\"color: #000000;font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\"><span style=\"background: rgb(255, 255, 255);\">SoiYun<\/span>\uff0cSangahLee\uff0cJong\tPilPark, ,et al. Modification of phage display technique for\timproved screening of high-affinity binding peptides[J].\tBiotechnology Volume 289, 10 January 2019, Pages 88-92.<\/span><\/p>\n<\/li>\n<li>\n<p style=\"margin-bottom: 0.11in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\"><span style=\"color: rgb(0, 0, 0);background: rgb(255, 255, 255);\">Yixiang\tXu , Georgina To\u2019a Salazar, &nbsp;Ningyan Zhang, &nbsp;Zhiqiang An.<\/span>\t<span style=\"color: rgb(0, 0, 0);\"><span style=\"background: rgb(255, 255, 255);\">T-cell\treceptor mimic (TCRm) antibody therapeutics against intracellular\tproteins[J]. <\/span><em>Antibody\tTherapeutics<\/em><span style=\"background: rgb(255, 255, 255);\">,\tVolume 2, Issue 1, 1 January 2019, Pages 1\u201311.<\/span><\/span><\/span><\/p>\n<\/li>\n<li>\n<p style=\"margin-bottom: 0.11in;\"><span style=\"background: #FFFFFF;color: #000000;font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">Kroetsch\tA , Qiao C , Heavey M , et al. Engineered pH-dependent recycling\tantibodies enhance elimination of Staphylococcal enterotoxin B\tsuperantigen in mice[J]<\/span><\/p>\n<\/li>\n<\/ol>\n<\/section>\n<p style=\"max-width: 100%;min-height: 1em;letter-spacing: 0.544px;font-size: 16px;font-family: \u5fae\u8f6f\u96c5\u9ed1;line-height: 32px;text-align: center;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box;text-indent: 27px;letter-spacing: 0.54px;widows: 1;font-size: 18px;overflow-wrap: break-word !important;\"><span style=\"max-width: 100%;box-sizing: border-box;color: rgb(0, 209, 0);overflow-wrap: break-word !important;\">\u4f1a\u8bae\u63a8\u8350<\/span><\/strong><\/p>\n<p style=\"max-width: 100%;min-height: 1em;letter-spacing: 0.544px;font-size: 16px;font-family: \u5fae\u8f6f\u96c5\u9ed1;line-height: 32px;text-align: center;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><span style=\"max-width: 100%;box-sizing: border-box;color: rgb(0, 209, 0);overflow-wrap: break-word !important;\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzA5MDY4MDQ1NQ==&amp;mid=2649833891&amp;idx=1&amp;sn=d9bb5b15968ccaefb4dd637743b1df69&amp;chksm=88021c4bbf75955d4648aa267b7db72e894ebc9f5748ee27745b19051591a65203c1c5a274e9&amp;scene=21#wechat_redirect\" target=\"_blank\"  style=\"color: rgb(87, 107, 149);-webkit-tap-highlight-color: rgba(0, 0, 0, 0);max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\">\u5927\u5496\u9f50\u805a\u4e0a\u6d77|CAR-T\u7ec6\u80de\u6cbb\u7597\u7814\u53d1\u53ca\u751f\u4ea7\u5de5\u827a\u8bba\u575b<\/a><\/span><\/strong><\/p>\n<p style=\"max-width: 677px;box-sizing: border-box;min-height: 16px;font-variant-numeric: normal;font-variant-east-asian: normal;font-size: 16px;font-family: \u5fae\u8f6f\u96c5\u9ed1;letter-spacing: 0.54px;line-height: 27.2px;text-align: center;widows: 1;overflow-wrap: break-word !important;\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzA5MDY4MDQ1NQ==&amp;mid=2649833899&amp;idx=2&amp;sn=0d99c5bd6ab2e51d93d8d7b1234ed00d&amp;chksm=88021c43bf7595551d94b8eabdf1ba2dfcbbfe23b1a3ee4876d77cf7c69604dfd71d18730978&amp;scene=21#wechat_redirect\" target=\"_blank\"  style=\"color: rgb(87, 107, 149);text-decoration: underline;-webkit-tap-highlight-color: rgba(0, 0, 0, 0);max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\">\u5927\u5496\u9f50\u805a\u5317\u4eac|\u56fd\u9645\u751f\u7269\u6cbb\u7597\u4ea7\u4e1a\u5927\u4f1a<\/strong><\/a><\/p>\n<p style=\"max-width: 677px;box-sizing: border-box;min-height: 16px;font-variant-numeric: normal;font-variant-east-asian: normal;font-size: 16px;font-family: \u5fae\u8f6f\u96c5\u9ed1;letter-spacing: 0.54px;line-height: 27.2px;text-align: center;widows: 1;overflow-wrap: break-word !important;\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MjM5NTI3MDE0NQ==&amp;mid=2653621905&amp;idx=5&amp;sn=8fb8c002bc9fa9fc4323d951e10707c0&amp;chksm=bd24b5ff8a533ce9b71923a658e4a0051fe456ab01bb04ba897948ee75b399b003197b31bb05&amp;scene=21#wechat_redirect\" target=\"_blank\"   style=\"color: rgb(87, 107, 149);text-decoration: underline;-webkit-tap-highlight-color: rgba(0, 0, 0, 0);max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\">2019\u7b2c\u4e09\u5c4a\u56fd\u9645\u751f\u7269\u6cbb\u7597\u5927\u4f1a\u66a8\u5c55\u89c8\u4f1a<\/strong><\/a><br style=\"max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 677px;box-sizing: border-box;min-height: 16px;font-variant-numeric: normal;font-variant-east-asian: normal;font-size: 16px;font-family: \u5fae\u8f6f\u96c5\u9ed1;letter-spacing: 0.54px;line-height: 27.2px;text-align: center;widows: 1;overflow-wrap: break-word !important;\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MjM5NTI3MDE0NQ==&amp;mid=2653621916&amp;idx=3&amp;sn=397880b1bee3b113b44e34878ae21700&amp;chksm=bd24b5f28a533ce41eb22e6b310f4712bc2932dd9a9c29a2dc3fbcbab98363555526b14a683c&amp;scene=21#wechat_redirect\" target=\"_blank\"   style=\"color: rgb(87, 107, 149);text-decoration: underline;-webkit-tap-highlight-color: rgba(0, 0, 0, 0);max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\">\u517d\u7528\u75ab\u82d7\u4e3b\u9898\u66f4\u65b0|2019\u75ab\u82d7\u5de5\u827a\u4f18\u5316\u4e0e\u521b\u65b0\u7814\u53d1\u8bba\u575b<\/strong><\/a><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;font-family: 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