{"id":104009,"date":"2019-02-24T00:00:50","date_gmt":"2019-05-17T08:54:46","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/104009\/"},"modified":"2019-05-17T16:54:49","modified_gmt":"2019-05-17T08:54:49","slug":"fd245d6702","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/104009\/","title":{"rendered":"\u566c\u83cc\u4f53\u5c55\u793a\u539f\u7406\u548c\u5b9e\u8df5\uff1a\u6784\u67b6\u7bc7\uff08\u4e0a\uff09"},"content":{"rendered":"
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\u524d\u8a00<\/section>\n<\/section>\n<\/section>\n<\/section>\n
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\u6587\u5e93\u7684\u6784\u5efa\u548c\u566c\u83cc\u4f53\u7684\u6269\u589e\u5c55\u793a<\/section>\n<\/section>\n<\/section>\n

\u539f\u7406<\/span><\/p>\n

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\u6587\u5e93\u6784\u5efa\u7684\u7b80\u5355\u6d41\u7a0b<\/section>\n<\/section>\n<\/section>\n

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5.\u5229\u7528overlap PCR \u5c06\u8f7b\u94feV\u7247\u6bb5\u57fa\u56e0\u548c\u91cd\u94feV\u7247\u6bb5\u57fa\u56e0\u5e8f\u5217\u62fc\u63a5\u5230\u4e00\u5757\uff0c\u5305\u542b\u4e24\u8005\u4e4b\u95f4\u7684\u8fde\u63a5\u7684\u67d4\u6027\uff08G4<\/sub>S\uff09n<\/sub>\u7684linker\uff1b<\/span><\/p>\n

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\n
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\n
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1.Rondot S , Koch J , Breitling F , et al. A helper phage to improve single-chain antibody presentation in phage display[J]. Nature Biotechnology, 2001, 19(1):75-78.<\/span><\/p>\n

2.Tiller T , Schuster I , Doroth\u00e9e Deppe, et al. A fully synthetic human Fab antibody library based on fixed VH\/VL framework pairings with favorable biophysical properties[J]. mAbs, 2013, 5(3):26.<\/span><\/p>\n

3.Lloyd C , Lowe D , Edwards B , et al. Modelling the human immune response: performance of a 10 11 human antibody repertoire against a broad panel of therapeutically relevant antigens[J]. Protein Engineering Design & Selection Peds, 2009, 22(3):159-68.<\/span><\/p>\n

4.Schwimmer L J , Huang B , Giang H , et al. Discovery of diverse and functional antibodies from large human repertoire antibody libraries[J]. Journal of Immunological Methods, 2013, 391(1-2):60-71.<\/span><\/p>\n

5.Mingqian F, HejiaoB, et al. Construction and next-generation sequencing analysis of a large phage-displayed VNAR single-domain antibody library from six na\u00efve nurse sharks [J]. Antibody Therapeutics, Volume 2, Issue 1, 1 January 2019, Pages 1\u201311<\/span><\/p>\n

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