{"id":104336,"date":"2019-03-03T00:00:50","date_gmt":"2019-05-17T08:56:55","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/104336\/"},"modified":"2019-05-17T16:56:59","modified_gmt":"2019-05-17T08:56:59","slug":"55785963c9","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/104336\/","title":{"rendered":"\u4ece\u5317\u4eac\u5230\u4e16\u754c: CHO\u7ec6\u80de\u7684\u201c\u4e2d\u56fd\u5f80\u4e8b\u201d"},"content":{"rendered":"
\n

\"\u4ece\u5317\u4eac\u5230\u4e16\u754c:<\/p>\n

\u56fe\u7247\u6765\u6e90\uff1a<\/span><\/em>https:\/\/www.statnews.com<\/span><\/em><\/span> <\/span><\/p>\n

\u4f17\u6240\u5468\u77e5\uff0c\u4e2d\u56fd\u4ed3\u9f20\u5375\u5de2\uff08<\/span>CHO<\/span>\uff09\u7ec6\u80de\u662f\u751f\u4ea7\u86cb\u767d\u7c7b\u836f\u7269\u7684\u9996\u9009\u5bbf\u4e3b\u7ec6\u80de\uff0c\u56e0\u4e3a\u4e0e\u5176\u4ed6\u7cfb\u7edf\u76f8\u6bd4\uff0c<\/span>CHO<\/span>\u7ec6\u80de\u5177\u6709\u4ee5\u4e0b\u4f18\u70b9\uff1a\u2460<\/span>CHO <\/span>\u7ec6\u80de\u5bf9\u86cb\u767d\u6709\u51c6\u786e\u7684\u52a0\u5de5\u3001\u4fee\u9970\u529f\u80fd\uff0c\u56e0\u6b64\u5176\u8868\u8fbe\u7684\u86cb\u767d\u8d28\u7684\u751f\u7269\u5b66\u6d3b\u6027\u66f4\u63a5\u8fd1\u4e8e\u5929\u7136\u86cb\u767d\uff1b\u2461<\/span>CHO <\/span>\u7ec6\u80de\u8010\u53d7\u526a\u5207\u529b\u548c\u6e17\u900f\u538b\u7684\u80fd\u529b\u76f8\u5bf9\u8f83\u5f3a\uff0c\u53ef\u6839\u636e\u57f9\u517b\u8981\u6c42\u9009\u62e9\u53ef\u8d34\u58c1\u57f9\u517b\u6216\u60ac\u6d6e\u57f9\u517b\u7684\u65b9\u5f0f\uff1b\u2462\u6574\u5408\u5916\u6e90\u57fa\u56e0\u540e\u7684\u7ec6\u80de\u7a33\u5b9a\uff0c\u91cd\u7ec4\u57fa\u56e0\u80fd\u9ad8\u6548\u6269\u589e\u548c\u8868\u8fbe\uff1b\u2463\u8868\u8fbe\u7684\u76ee\u7684\u86cb\u767d\u53ef\u7531\u7ec6\u80de\u5185\u8fd0\u8f93\u5230\u7ec6\u80de\u5916\uff0c\u5e76\u4e14<\/span>CHO <\/span>\u7ec6\u80de\u53ea\u8868\u8fbe\u5c11\u91cf\u7684\u5185\u6e90\u86cb\u767d\uff0c\u6709\u5229\u4e8e\u76ee\u7684\u86cb\u767d\u7684\u63d0\u53d6\u3002\u5982\u4eca\uff0c\u5e02\u573a\u4e0a\u4e00\u534a\u4ee5\u4e0a\u6700\u4e3a\u7545\u9500\u7684\u751f\u7269\u5236\u5242\u662f\u7531<\/span>CHO<\/span>\u7ec6\u80de\u4ea7\u751f\u7684\u3002\u5982\uff0c\u963f\u8fbe\u6728\u5355\u6297\uff08<\/span>Humira<\/span>\uff09\u3001\u8d1d\u4f10\u5355\u6297\uff08<\/span>Avastin<\/span>\uff09\u548c\u5229\u59a5\u6614\u5355\u6297\uff08<\/span>Rituxan<\/span>\uff09\u3002\u53ef\u4ee5\u8bf4\uff0c<\/span>CHO<\/span>\u7ec6\u80de\u5bf9\u73b0\u4ee3\u751f\u7269\u836f\u5de5\u4e1a\u53d1\u5c55\u505a\u51fa\u4e86\u4e0d\u53ef\u78e8\u706d\u7684\u8d21\u732e\u3002\u4e0d\u8fc7\u4f60\u662f\u5426\u77e5\u9053<\/span>CHO<\/span>\u7ec6\u80de\u6700\u65e9\u7684\u5386\u53f2\uff1f\u4eca\u5929\uff0c\u8ba9\u6211\u4eec\u7b80\u5355\u68b3\u7406\u4e00\u4e0b<\/span>CHO<\/span>\u7ec6\u80de\u7684\u524d\u4e16\u4eca\u751f\uff0c\u4e86\u89e3\u4e00\u4e0b<\/span>CHO<\/span>\u7ec6\u80de\u80cc\u540e\u7684\u6545\u4e8b\uff0c\u6216\u8bb8\u53ef\u4ee5\u7ed9\u6211\u4eec\u672a\u6765\u7684\u5de5\u4f5c\u66f4\u591a\u542f\u53d1\u548c\u601d\u8003\u3002<\/span><\/span><\/p>\n

 <\/span><\/p>\n

\"\u4ece\u5317\u4eac\u5230\u4e16\u754c:<\/p>\n

\u56fe\u7247\u6765\u6e90\uff1a<\/span><\/em><Recombinant protein therapeutics fromCHO cells – 20 years and counting><\/span><\/em><\/span><\/p>\n

 <\/span>\u4e2d\u56fd\u4ed3\u9f20\uff08\u5b66\u540d<\/span> Cricetulus griseus<\/span>\uff09\u5c5e\u4e8e\u6765\u81ea\u4e8e\u4e2d\u56fd\u5317\u90e8\u548c\u8499\u53e4\u5730\u533a\u7684\u556e\u9f7f\u52a8\u7269\u5bb6\u65cf\u3002<\/span>1919<\/span>\u5e74\uff0c\u5317\u4eac\u534f\u548c\u533b\u5b66\u9662\u7684\u80e1\u6b63\u7965\u6559\u6388\uff08\u6ce8\uff1a\u80e1\u6b63\u7965\uff0c<\/span>1896<\/span>\u5e74\u751f\uff0c\u8457\u540d\u75c5\u7406\u5b66\u5bb6\u3002<\/span>1921<\/span>\u5e74\u6bd5\u4e1a\u4e8e\u7f8e\u56fd\u54c8\u4f5b\u533b\u5b66\u9662\uff0c\u540e\u5728\u7f8e\u56fd\u9ebb\u7701\u603b\u533b\u9662\u7ee7\u7eed\u5b66\u4e60\u75c5\u7406\u5b66<\/span>5<\/span>\u5e74\u3002\u56de\u56fd\u540e\u5386\u4efb\u5317\u4eac\u534f\u548c\u533b\u5b66\u9662\u526f\u9662\u957f\uff0c\u75c5\u7406\u7cfb\u6559\u6388\u3001\u7cfb\u4e3b\u4efb\u7b49\u804c\uff0c\u5728\u75c5\u7406\u5b66\u7814\u7a76\u4e2d\u9887\u6709\u5efa\u6811\u3002\uff09\u6700\u65e9\u5728\u5317\u4eac\u4f7f\u7528\u4e2d\u56fd\u5730\u4ed3\u9f20\u7814\u7a76\u80ba\u708e\u7403\u83cc\uff0c\u7531\u4e8e\u6761\u4ef6\u6240\u9650\uff0c\u8bd5\u9a8c\u5c0f\u767d\u9f20\u5f88\u96be\u83b7\u5f97\uff0c\u6b63\u597d\u5317\u4eac\u5468\u56f4\u7684\u4e2d\u56fd\u5730\u4ed3\u9f20\u4f17\u591a\uff0c\u4e2d\u56fd\u5730\u4ed3\u9f20\u4fbf\u6210\u4e3a\u4e86\u5f88\u597d\u7684\u8bd5\u9a8c\u7814\u7a76\u5de5\u5177\u3002\u540e\u6765\u5728\u5317\u4eac\u534f\u548c\u533b\u5b66\u9662\u5de5\u4f5c\u7684\u7f8e\u56fd\u79d1\u5b66\u5bb6<\/span>Jocelyn Smyly<\/span>\u548c<\/span>Charles Young<\/span>\u53d1\u73b0\u4e2d\u56fd\u5730\u4ed3\u9f20\u5f88\u5bb9\u6613\u611f\u67d3\u5bc4\u751f\u866b\u5229\u4ec0\u66fc\u539f\u866b\u4ece\u800c\u5f15\u53d1<\/span>Black fever<\/span>\u9ed1\u70ed\u75c5\uff0c\u4e4b\u540e\u4e2d\u56fd\u7684\u533b\u5b66\u79d1\u7814\u4eba\u5458\u90fd\u4f7f\u7528\u4e2d\u56fd\u5730\u4ed3\u9f20\u6765\u7814\u7a76\u5404\u79cd\u4f20\u67d3\u6027\u75be\u75c5\uff0c\u4e2d\u56fd\u4ed3\u9f20\u6210\u4e3a\u4e86\u6d41\u884c\u75c5\u5b66\u7814\u7a76\u7684\u6709\u529b\u5de5\u5177\u3002<\/span><\/p>\n

<\/span><\/p>\n

\"\u4ece\u5317\u4eac\u5230\u4e16\u754c:<\/p>\n

\u56fe\u7247\u6765\u6e90\uff1a<\/em>www.acib.at<\/span><\/em><\/span><\/p>\n

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1948<\/span>\u5e74<\/span>12<\/span>\u6708\uff0c\u5357\u4eac\u89e3\u653e\u524d\u7684\u4e00\u4e2a\u9ed1\u6697\u7684\u6df1\u591c\uff0c<\/span>\u7f8e\u56fd\u6d1b\u514b\u83f2\u52d2\u57fa\u91d1\u4f1a\u56fd\u9645\u533b\u7597\u90e8\uff08<\/span>Rockefeller Foundation\u2019s International Health Division<\/span>\uff09\u533b\u751f<\/span>Dr. Robert Briggs Watson<\/span>\u5192\u7740\u751f\u547d\u5371\u9669\uff0c\u5e26\u7740\u4e00\u7bb1<\/span>20<\/span>\u4e2a\u5317\u4eac\u80e1\u6b63\u7965\u6559\u6388\u8d60\u4e0e\u7684\u3001\u7528\u6765\u7ee7\u7eed\u7814\u7a76\u5728\u4e9a\u6d32\u8086\u8650\u7684\u759f\u75be\u7684\u4e2d\u56fd\u5730\u4ed3\u9f20\uff0c\u8eb2\u8fc7\u4ea4\u6218\u533a\uff0c\u9a71\u8f66\u5230\u4e0a\u6d77\u673a\u573a\u3002\u5c3d\u7ba1\u5bd2\u51b7\u7684\u96e8\u6ef4\u5f3a\u70c8\u62cd\u6253\u7740\u73bb\u7483\uff0c\u6ce5\u77f3\u6d41\u968f\u65f6\u53ef\u4ee5\u963b\u65ad\u9053\u8def\uff0c\u6700\u7ec8<\/span>Watson<\/span>\u8fd8\u662f\u6210\u529f\u5730\u767b\u4e0a\u4e86<\/span>Pan Am<\/span>\u822a\u73ed\u79bb\u5f00\u4e86\u4e2d\u56fd\uff0c\u5c06\u8fd9\u6279\u4e2d\u56fd\u5730\u4ed3\u9f20\u8fd0\u7ed9\u7ebd\u7ea6\u7684\u8bd5\u9a8c\u52a8\u7269\u4e13\u5bb6<\/span>Victor Schwentker<\/span>\u3002\u8c01\u4e5f\u6ca1\u6709\u60f3\u5230\uff0c\u5f88\u591a\u5e74\u540e\uff0c\u4f34\u968f\u4ed6\u7684<\/span>20<\/span>\u53ea\u4e2d\u56fd\u5730\u4ed3\u9f20\uff0c\u4f1a\u6210\u4e3a\u5f53\u4ee3\u836f\u7269\u751f\u4ea7\u5de5\u4e1a\u7684\u5173\u952e<\/span>\u201c<\/span>\u94a5\u5319<\/span>\u201d<\/span>\u3002<\/span><\/span><\/p>\n

 <\/span><\/p>\n

\u7f8e\u56fd\u79d1\u5b66\u5bb6\u540e\u6765\u5b8c\u6210\u4e86\u4e2d\u56fd\u5730\u4ed3\u9f20\u7684\u7e41\u80b2\uff0c\u4f7f\u5176\u6210\u4e3a\u79d1\u5b66\u7814\u7a76\u7684\u6709\u529b\u5de5\u5177\u3002<\/span>University of Colorado Medical Center<\/span>\u7684<\/span>Dr.Theodore T. Puck<\/span>\u548c\u4ed6\u7684\u4e0b\u5c5e\u540c\u4e8b<\/span>Fa-Ten Kao<\/span>\u5728<\/span>1957<\/span>\u5e74\u4ece\u6ce2\u58eb\u987f\u764c\u75c7\u7814\u7a76\u4e2d\u5fc3\u7684<\/span>Georege Yerganian<\/span>\u535a\u58eb\u7684\u5b9e\u9a8c\u5ba4\u83b7\u5f97\u4e86\u4e00\u4e2a\u96cc\u6027\u4e2d\u56fd\u5730\u4ed3\u9f20\uff0c\u5e76\u6210\u529f\u5206\u79bb\u4e86<\/span>CHO<\/span>\u7ec6\u80de\u682a\uff0c\u7531\u4e8e\u8be5\u7ec6\u80de\u5feb\u901f\u60ac\u6d6e\u751f\u957f\u548c\u9ad8\u86cb\u767d\u8868\u8fbe\u7684\u7279\u6027\uff0c<\/span>CHO<\/span>\u7ec6\u80de\u5f00\u59cb\u5728\u79d1\u7814\u548c\u4f01\u4e1a\u83b7\u5f97\u666e\u904d\u7684\u5e94\u7528\u3002<\/span><\/span><\/p>\n


<\/em><\/p>\n

<\/em><\/p>\n

\"\u4ece\u5317\u4eac\u5230\u4e16\u754c:<\/p>\n

\u56fe\u7247\u6765\u6e90\uff1a<\/em>https:\/\/www.allacronyms.com<\/span><\/em><\/span>
<\/p>\n

 <\/span><\/em><\/strong><\/p>\n

1984<\/span>\u5e74\uff0c<\/span>Genentech<\/span>\u516c\u53f8\u9996\u6b21\u5b9e\u73b0\u91cd\u7ec4\u4e2d\u56fd\u4ed3\u9f20\u5375\u5de2\u7ec6\u80de\u8868\u8fbe\u7ec4\u7ec7\u578b\u7ea4\u6eb6\u9176\u539f\u6fc0\u6d3b\u5242<\/span>(t-PA)<\/span>\u5e76\u4e8e<\/span>1987<\/span>\u5e74\u6210\u529f\u83b7\u6279\u4e0a\u5e02\uff0c\u6807\u5fd7\u7740\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u8868\u8fbe\u7cfb\u7edf\u751f\u4ea7\u86cb\u767d\u836f\u7269\u7684\u4e00\u4e2a\u6807\u5fd7\u6027\u4e8b\u4ef6\u3002\u968f\u540e\uff0c\u8bb8\u591a\u5916\u6e90\u86cb\u767d\u57fa\u56e0\u76f8\u7ee7\u88ab\u8f6c\u67d3\u5230\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\uff0c\u4e00\u4e9b\u6709\u4ef7\u503c\u7684\u86cb\u767d\u4e0d\u65ad\u5b9e\u73b0\u8868\u8fbe\uff0c\u5305\u62ec\u51dd\u8840\u56e0\u5b50\u3001\u4fc3\u7ea2\u7ec6\u80de\u751f\u6210\u7d20<\/span>(EPO)<\/span>\u3001\u514d\u75ab\u7403\u86cb\u767d\u3001\u5c3f\u6fc0\u9176\u3001\u4e59\u809d\u8868\u9762\u6297\u539f\uff08<\/span>HBsAg<\/span>\uff09\u548c\u5355\u514b\u9686\u6297\u4f53\u7b49\uff0c\u6781\u5927\u5730\u4fc3\u8fdb\u4e86\u751f\u7269\u836f\u5de5\u4e1a\u7684\u53d1\u5c55\u3002\u540c\u65f6\uff0c\u968f\u7740<\/span>CHO<\/span>\u7ec6\u80de\u5728\u5b9e\u9a8c\u5ba4\u7684\u666e\u53ca\uff0c\u79d1\u5b66\u5bb6\u6210\u529f\u5206\u79bb\u51fa\u7684\u4e0d\u540c\u4e9a\u578b\u7684<\/span>CHO<\/span>\u7ec6\u80de\u682a\uff0c\u6bd4\u5982<\/span>CHO-S, , CHO DXB11, CHO DG44<\/span>\uff0c<\/span> CHO-M<\/span>\u4ee5\u53ca\u8fd1\u5e74\u6765\u53d7\u5230\u6301\u7eed\u5173\u6ce8\u7684<\/span>GS<\/span>\u57fa\u56e0\u6572\u9664\u7684<\/span>CHO<\/span>\u7ec6\u80de\uff08\u5982<\/span>Merck\/Sigma Aldrich<\/span>\u516c\u53f8\u7684<\/span>CHOZN, Lonza<\/span>\u7684<\/span>CHO GS Xceed, Horizon<\/span>\u516c\u53f8\u7528<\/span>rAAV<\/span>\u6280\u672f\u6572\u9664\u7684<\/span>CHO<\/span>\u7ec6\u80de\uff09\u3002<\/span><\/span><\/p>\n

<\/span><\/p>\n

\"\u4ece\u5317\u4eac\u5230\u4e16\u754c:<\/p>\n

\"\u4ece\u5317\u4eac\u5230\u4e16\u754c:<\/p>\n

\u56fe\u7247\u6765\u6e90<\/span><\/em>: <\/span><\/em>https:\/\/www.moleculardevices.com<\/span><\/span>
<\/p>\n

 <\/span><\/em><\/p>\n

\u4e0d\u8fc7\u9057\u61be\u7684\u662f\uff0c\u7531\u4e8e\u5f53\u65f6\u7279\u6b8a\u7684\u5386\u53f2\u80cc\u666f\uff0c<\/span>Robert Watson<\/span>\u540e\u6765\u88ab\u4e2d\u56fd\u7ec6\u83cc\u6218\u59d4\u5458\u4f1a\u6307\u63a7\u4e3a\u6218\u4e89\u7f6a\u72af\uff0c\u8fd9\u4e5f\u95f4\u63a5\u5bfc\u81f4\u4e86\u534f\u548c\u533b\u5b66\u9662\u80e1\u6b63\u7965\u6559\u6388\u7684\u5165\u72f1\uff0c\u80e1\u6559\u6388\u540e\u6765\u88ab\u4f5c\u4e3a\u201c\u53cd\u52a8\u5b66\u672f\u6743\u5a01\u201d\u906d\u6279\u6597\u8feb\u5bb3\uff0c<\/span>1966<\/span>\u5e74\uff0c\u80e1\u6b63\u7965\u592b\u5987\u5148\u540e\u5728\u5bb6\u4e2d\u81ea\u6740\u3002\u5982\u4eca\u5728\u548c\u5e73\u5e74\u4ee3\uff0c\u629a\u4eca\u8ffd\u6614\uff0c\u6211\u4eec\u7f05\u6000\u4e3a\u4eba\u7c7b\u5065\u5eb7\u4e8b\u4e1a\u505a\u51fa\u5de8\u5927\u8d21\u732e\u7684\u8001\u4e00\u8f88\u79d1\u5b66\u5bb6\u4eec\uff0c\u4e5f\u66f4\u52a0\u73cd\u60dc\u6765\u4e4b\u4e0d\u6613\u7684\u4e00\u5207<\/span><\/span>\u3002<\/span><\/p>\n

 <\/span><\/p>\n

\u53e6\u4e00\u65b9\u9762\uff0c\u4f34\u968f\u7740<\/span>CHO<\/span>\u7ec6\u80de\u5e94\u7528\u7684\u666e\u53ca\uff0c<\/span>CHO<\/span>\u7ec6\u80de\u57f9\u517b\u57fa\u4ea7\u4e1a\u4e5f\u5f97\u5230\u4e86\u5145\u5206\u7684\u7814\u7a76\u548c\u5feb\u901f\u7684\u53d1\u5c55\u3002\u4f20\u7edf\u4e0a<\/span>CHO<\/span>\u7ec6\u80de\u7684\u57f9\u517b\u662f\u5728<\/span>DMEM\/F12<\/span>\u57fa\u7840\u57f9\u517b\u57fa\u4e2d\u6dfb\u52a0<\/span>5-10%<\/span>\u7684\u80ce\u725b\u8840\u6e05\u5b8c\u6210\u7684\uff0c\u8840\u6e05\u9664\u4e86\u4f9b\u7ed9\u7ec6\u80de\u7684\u8425\u517b\u6210\u5206\u5916\uff0c\u8fd8\u63d0\u4f9b\u4e86\u9898\u4e3a\u57f9\u517b\u7ec6\u80de\u589e\u6b96\u6240\u5fc5\u987b\u7684\u751f\u957f\u56e0\u5b50\uff0c\u4f46\u8840\u6e05\u7684\u4f7f\u7528\u5b58\u5728\u6279\u6b21\u95f4\u5dee\u5f02\u5927\uff0c\u6613\u88ab\u652f\u539f\u4f53\u548c\u75c5\u6bd2\u7b49\u6c61\u67d3\uff0c\u6210\u672c\u9ad8\uff0c\u4e0d\u5229\u7528\u4ea7\u54c1\u5206\u79bb\u7eaf\u5316\u7b49\u5f88\u591a\u5f0a\u7aef\uff0c\u4ee5\u53ca\u5f71\u54cd\u7ec6\u80de\u7684\u751f\u957f\u53ca\u6700\u7ec8\u4ea7\u7269\u7684\u8d28\u91cf\uff0c\u4e0d\u9002\u4e8e\u5927\u89c4\u6a21\u5de5\u4e1a\u5316\u751f\u4ea7\u3002\u6240\u4ee5\u4ece<\/span>70<\/span>\u5e74\u5f00\u59cb\u79d1\u5b66\u5bb6\u5c31\u5f00\u59cb\u7814\u7a76\u548c\u5f00\u53d1\u5de5\u4e1a\u65e0\u8840\u6e05\u57f9\u517b\u57fa\u3002\u65e0\u8840\u6e05\u57f9\u517b\u57fa\u7684\u5f00\u53d1\u5927\u5927\u63d0\u9ad8\u4e86<\/span>CHO<\/span>\u7ec6\u80de\u57f9\u517b\u5de5\u827a\u7684\u4fbf\u5229\u6027\uff0c\u53ef\u91cd\u590d\u6027\u4ee5\u53ca\u964d\u4f4e\u4e86\u4ea7\u54c1\u7eaf\u5316\u538b\u529b\u3002\u7ecf\u8fc7\u51e0\u5341\u5e74\u7684\u53d1\u5c55\uff0c\u52a8\u7269\u7ec6\u80de\u65e0\u8840\u6e05\u57f9\u517b\u57fa\u7ecf\u5386\u4e86\u52a8\u7269\u86cb\u767d\u6c34\u89e3\u7269\u57f9\u517b\u57fa\uff0c\u690d\u7269\u86cb\u767d\u6c34\u89e3\u7269\u57f9\u517b\u57fa\u5230\u65e0\u8840\u6e05\u5316\u5b66\u6210\u5206\u9650\u5b9a\u7684\u57f9\u517b\u57fa\u7b49\u51e0\u4e2a\u9636\u6bb5\u3002\u5178\u578b\u7684\u65e0\u8840\u6e05\u57f9\u517b\u57fa\u542b\u6709<\/span>50-70<\/span>\u4e2d\u6210\u5206\uff0c\u5305\u62ec\u6c28\u57fa\u9178\uff0c\u7ef4\u751f\u7d20\uff0c\u5fae\u91cf\u5143\u7d20\uff0c\u751f\u957f\u56e0\u5b50\uff0c\u65e0\u673a\u76d0\uff0c\u7f13\u51b2\u4f53\u7cfb\u7b49\u3002\u5411\u57f9\u517b\u57fa\u4e2d\u6dfb\u52a0\u52a8\u7269\u6216\u690d\u7269\u7684\u86cb\u767d\u6c34\u89e3\u7269\u53ef\u4ee5\u6709\u6548\u5730\u63d0\u9ad8\u7ec6\u80de\u751f\u957f\u5bc6\u5ea6\uff0c\u5ef6\u957f\u7ec6\u80de\u6d3b\u6027\uff0c\u4ece\u800c\u63d0\u9ad8\u6297\u4f53\u8868\u8fbe\u91cf\uff0c\u5e38\u89c1\u7684\u6709\u5927\u8c46\u3001\u9175\u6bcd\u548c\u5c0f\u9ea6\u86cb\u767d\u6c34\u89e3\u7269\u7b49\uff0c\u4f46\u662f\u7531\u4e8e\u5176\u6210\u5206\u7684\u590d\u6742\u6027\u548c\u6279\u6b21\u95f4\u7684\u8d28\u91cf\u5dee\u5f02\uff0c\u6dfb\u52a0\u86cb\u767d\u6c34\u89e3\u7269\u53ef\u80fd\u4f1a\u9020\u6210\u57f9\u517b\u57fa\u6279\u6b21\u95f4\u4e0d\u7a33\u5b9a\u4ece\u800c\u5f71\u54cd\u57f9\u517b\u8fc7\u7a0b\u548c\u4ea7\u54c1\u8d28\u91cf\u3002\u6240\u4ee5\uff0c\u76ee\u524d\u53ca\u672a\u6765\u65e0\u8840\u6e05\u57f9\u517b\u57fa\u7684\u8d8b\u52bf\u662f\u91c7\u7528\u65e0\u52a8\u7269\u6e90\u3001\u5316\u5b66\u6210\u5206\u786e\u5b9a\u7684\u914d\u65b9\u8fdb\u884c\u751f\u7269\u5236\u836f\u751f\u4ea7\u3002<\/span><\/span><\/p>\n


<\/p>\n

\u7531\u4e8e\u5728\u7814\u53d1\u5b9e\u529b\u3001\u8d28\u91cf\u4f53\u7cfb\u3001\u751f\u4ea7\u63a7\u5236\u548c\u54c1\u724c\u5f71\u54cd\u529b\u7b49\u65b9\u9762\u7684\u4f18\u52bf\uff0c\u5916\u4f01\u57f9\u517b\u57fa\u516c\u53f8\u5360\u636e\u4e86\u5168\u7403\u7ec6\u80de\u57f9\u517b\u57fa\u5e02\u573a\u7684\u7edd\u5927\u90e8\u5206\u4efd\u989d\uff0c\u76ee\u524d\u56fd\u5916\u77e5\u540d\u7684<\/span>CHO<\/span>\u7ec6\u80de\u57f9\u517b\u57fa\u516c\u53f8\u6709<\/span>Gibco<\/span>\uff08<\/span>Now part of Therm Fisher<\/span>\uff09<\/span>, SAFC(Now part of Merck),  Hyclone(Now part of GE Healthcare), Irvine Scientific\uff08A member of JXTG Group\uff09,  Lonza<\/span>\u7b49\uff0c\u652f\u6491\u4e86\u5168\u7403\u4e0a\u5e02\u7684\u4f17\u591a\u751f\u7269\u836f\u7684\u5546\u4e1a\u5316\u751f\u4ea7\u3002\u540c\u65f6\uff0c\u4e0d\u540c\u4f9b\u5e94\u5546\u4e5f\u5728\u52aa\u529b\u63d0\u9ad8\u57f9\u517b\u57fa\u7814\u53d1\u548c\u751f\u4ea7\u7684\u6280\u672f\u80fd\u529b\uff0c\u57fa\u4e8e\u4e0d\u540c\u7684\u7ec6\u80de\u57f9\u517b\u5e94\u7528\uff0c\u4e0d\u65ad\u66f4\u65b0\u5347\u7ea7\u6362\u4ee3\u57f9\u517b\u57fa\u7684\u914d\u65b9\u548c\u914d\u7f6e\u65b9\u6cd5\u3002\u6bd4\u5982<\/span>Thermo Fisher\/Gibco<\/span>\u901a\u8fc7\u4e13\u5229\u7684<\/span>AGT<\/span>\u6280\u672f\uff0c\u4f7f\u5e72\u7c89\u57f9\u517b\u57fa\u7684\u914d\u7f6e\u8fc7\u7a0b\u53d8\u5f97\u4fbf\u6377\u3001\u7b80\u5355\u3002<\/span>Merck\/SAFC<\/span>\u53bb\u5e74\u5ba3\u5e03\u4e0a\u5e02\u4e86\u7b2c\u4e00\u4e2a\u5546\u4e1a\u5316\u7684\u7528\u4e8e\u704c\u6ce8\u5de5\u827a\u57f9\u517b\u7684\u57f9\u517b\u57fa\uff0c\u6765\u5e94\u5bf9\u704c\u6ce8\u57f9\u517b\u5de5\u827a\u7684\u6311\u6218\uff0c\u7f8e\u56fd<\/span>Irvine Scientific<\/span>\u516c\u53f8\u4e5f\u65b0\u63a8\u51fa\u4e86\u7528\u4e8e<\/span>GMP-ready <\/span>\u7684<\/span>CHO<\/span>\u7ec6\u80de\u704c\u6ce8\u57f9\u517b\u57fa\u53ca\u7528\u4e8e<\/span>Fed-batch<\/span>\u57f9\u517b\u7684\u9ad8\u6548\u6d53\u7f29\u7684\u8865\u6599<\/span>BalanCD CHO Feed 4<\/span>\uff0c\u5e76\u4e14<\/span>Feed 4<\/span>\u5e72\u7c89\u914d\u7f6e\u8fc7\u7a0b\u540c\u6837\u7b80\u5355\uff0c\u52a0\u6c34\u6eb6\u89e3\uff0c\u65e0\u9700\u8c03\u8282<\/span>pH<\/span>\uff0c\u4fbf\u6377\u7684\u914d\u7f6e\u65b9\u6848\u5bf9\u751f\u7269\u836f\u5de5\u4e1a\u5927\u89c4\u6a21\u751f\u4ea7\u5177\u6709\u5f88\u5927\u7684\u5438\u5f15\u529b\u3002<\/span><\/span><\/p>\n

 <\/span><\/em><\/span><\/p>\n

\u751f\u7269\u533b\u836f\u884c\u4e1a\u662f\u4e2d\u56fd\u6218\u7565\u6027\u65b0\u5174\u4ea7\u4e1a\uff0c\u56fd\u5185\u751f\u7269\u5236\u836f\u884c\u4e1a\u7ecf\u8fc7\u591a\u5e74\u7684\u53d1\u5c55\uff0c\u76ee\u524d\u4e5f\u6709\u4e00\u6279\u672c\u571f\u516c\u53f8\u4e13\u6ce8\u4e8e\u5f00\u53d1\u65e0\u8840\u6e05\u57f9\u517b\u57fa\u548c\u4f18\u5316\u7ec6\u80de\u57f9\u517b\u5de5\u827a\uff0c\u4e5f\u53d6\u5f97\u4e86\u53ef\u559c\u7684\u8fdb\u5c55\u3002\u7531\u4e8e\u4e0d\u540c\u7ec6\u80de\u682a\u7684\u4ee3\u8c22\u9700\u6c42\u4e0d\u5b8c\u5168\u4e00\u6837\uff0c\u6240\u4ee5\u5f88\u96be\u5f00\u53d1\u51fa\u4e00\u6b3e\u80fd\u591f\u6ee1\u8db3\u6240\u6709\u7ec6\u80de\u5e94\u7528\u9700\u6c42\u7684\u201c\u901a\u7528\u578b\u201d<\/span>CHO<\/span>\u7ec6\u80de\u57f9\u517b\u57fa\uff0c\u540c\u65f6\u4e3a\u4e86\u5927\u89c4\u6a21\u751f\u4ea7\u9636\u6bb5\u6210\u672c\u548c\u5de5\u827a\u548c\u98ce\u9669\u63a7\u5236\u7684\u8003\u8651\uff0c\u5f88\u591a\u516c\u53f8\u8fd8\u662f\u4f1a\u9009\u62e9\u5728\u9879\u76ee\u8fdb\u5c55\u5230\u4e00\u5b9a\u7a0b\u5ea6\u540e\u5f00\u53d1\u4e2a\u6027\u5316\u5b9a\u5236\u57f9\u517b\u57fa\uff0c\u672a\u6765\u57f9\u517b\u57fa\u7684\u5927\u89c4\u6a21\u751f\u4ea7\u6280\u672f<\/span> \u2013 <\/span>\u80fd\u5426\u4fdd\u8bc1\u5927\u89c4\u6a21\u57f9\u517b\u57fa\u5e72\u7c89\u7684\u8d28\u91cf\u548c\u6279\u6b21\u7a33\u5b9a\u6027\uff0c\u4e5f\u5c06\u6210\u4e3a\u57f9\u517b\u57fa\u516c\u53f8\u7684\u6838\u5fc3\u7ade\u4e89\u529b\u4e4b\u4e00\u3002<\/span><\/span><\/p>\n

 <\/span><\/p>\n

\u603b\u4e4b\uff0c<\/span>CHO<\/span>\u7ec6\u80de\u7684\u51fa\u73b0\u7ed9\u6574\u4e2a\u5168\u7403\u751f\u7269\u5236\u836f\u4ea7\u4e1a\u6ce8\u5165\u4e86\u65e0\u7a77\u7684\u6d3b\u529b\uff0c\u4ea7\u751f\u4e86\u5de8\u5927\u7684\u79d1\u7814\u548c\u7ecf\u6d4e\u4ef7\u503c\uff0c\u5e76\u5c06\u4e00\u76f4\u9020\u798f\u4eba\u7c7b\uff0c<\/span>\u540c\u65f6\uff0c\u4f7f\u7528CHO\u7ec6<\/span>\u80de\u5e73\u53f0\u8868\u8fbe\u751f\u7269\u836f\u8fd8\u9762\u4e34\u8fd9\u4e00\u4e9b\u6311\u6218\uff0c\u9700\u8981\u5b66\u672f\u548c\u5de5\u4e1a\u754c\u4e00\u8d77\u52aa\u529b\uff0c\u4ece\u7efc\u5408\u5229\u7528\u79d1\u5b66\u548c\u5de5\u7a0b\u7684\u77e5\u8bc6\u6765\u89e3\u51b3\uff0c\u6bd4\u5982<\/span><\/span><\/p>\n

 <\/span>\u2460\u6784\u5efa\u7684\u91cd\u7ec4<\/span>CHO<\/span>\u7ec6\u80de\u751f\u4ea7\u6548\u7387\u4f4e\uff0c\u4ea7\u7269\u6d53\u5ea6\u4ea6\u4f4e\uff0c\u57f9\u517b\u5de5\u827a\u590d\u6742\uff0c\u800c\u4e14<\/span>CHO<\/span>\u7ec6\u80de\u682a\u7684\u7a33\u5b9a\u6027\u8fd8\u8f83\u5dee\uff1b<\/span><\/span><\/p>\n

\u2461\u67d0\u4e9b\u7cd6\u57fa\u5316\u8868\u8fbe\u4ea7\u7269\u4e0d\u7a33\u5b9a\uff0c\u4e0d\u6613\u7eaf\u5316\uff1b<\/span> <\/span><\/span><\/p>\n

\u2462\u91cd\u7ec4<\/span>CHO<\/span>\u7ec6\u80de\u4e0a\u6e38\u6784\u5efa\u4e0e\u4e0b\u6e38\u5206\u79bb\u7eaf\u5316\u8131\u8282\uff0c\u4e3b\u8981\u8868\u73b0\u5728\u4e0a\u6e38\u6784\u5efa\u65f6\u7740\u91cd\u8003\u8651\u5b83\u7684\u9ad8\u6548\u8868\u8fbe\uff0c\u800c\u5bf9\u9ad8\u8868\u8fbe\u7684\u4ea7\u7269\u7684\u5206\u79bb\u7eaf\u5316\u8fc7\u7a0b\u8003\u8651\u8f83\u5c11\uff1b<\/span> <\/span><\/span><\/p>\n

\u2463\u91cd\u7ec4\u7ec6\u80de\u57f9\u517b\u8d39\u7528\u6602\u8d35\uff0c\u81ea\u52a8\u5316\u6c34\u5e73\u4f4e\u4e0b\u3002<\/span> <\/span><\/span><\/p>\n

 <\/span><\/p>\n

\"\u4ece\u5317\u4eac\u5230\u4e16\u754c:<\/p>\n

\u56fe\u7247\u6765\u6e90: <CHO cells canmake more protein>. 2017<\/span><\/em><\/span><\/p>\n

 <\/span>
<\/p>\n

 <\/span>\u968f\u7740\u6280\u672f\u7684\u53d1\u5c55\u548c\u6211\u56fd\u5bf9\u751f\u7269\u836f\u4ea7\u4e1a\u6295\u8d44\u652f\u6301\u529b\u5ea6\u7684\u52a0\u5927\uff0c\u6211\u4eec\u4e5f\u8877\u5fc3\u5e0c\u671b\uff0c\u6709\u671d\u4e00\u65e5\u6211\u4eec\u80fd\u8bde\u751f\u771f\u6b63\u5c5e\u4e8e\u6211\u4eec\u81ea\u5df1\u77e5\u8bc6\u4ea7\u6743\u7684\u201c<\/span>CHO<\/span>\u7ec6\u80de\u201d\u53ca\u76f8\u5173\u521b\u65b0\u4ea7\u54c1\uff0c\u66f4\u597d\u5730\u8fce\u63a5\u6211\u4eec\u751f\u7269\u5236\u836f\u4ea7\u4e1a\u7684\u65b0\u65f6\u4ee3\u3002<\/span><\/span><\/p>\n


<\/span><\/p>\n

\u53c2\u8003\u8d44\u6599\uff1a<\/span><\/strong><\/p>\n

Rawley JD.Theodore T. Puck (September 24, 1916\u2013November 6, 2005). American Journal HumanGenetics 2006;78(3): 365\u2013366.<\/span><\/p>\n

Kim JY et al. CHO cells inbiotechnology for production of recombinant proteins: current state and furtherpotential. Applied Microbiology and Biotechnology 2012;93(3): 917\u2013930.<\/span><\/p>\n

Urlaub G; Chasin LA. Isolation of Chinesehamster cell mutants deficient in dihydrofolate reductase activity[J].Proceedings of the National Academy of Sciences U.S.A., 1980-07, 77 (7):4216-4220.<\/span><\/p>\n

Landauer K, Woischnigg H, Hepp N et al (2011)Development of a chemically defined CHO medium by engineering based on a feed solution.BMC Proc 5(Suppl 8):P41<\/span><\/p>\n

CHO MEDIA PLATFORM FACILITATES INTEGRATED CELLLINE DEVELOPMENT AND MEDIA OPTIMIZATION, IrvineScientic Poster in ESACT 2017<\/span><\/p>\n

\u5f6d\u96f7<\/span>. <\/span>\u6781\u7b80\u65b0\u836f\u53d1\u73b0\u53f2<\/span>. <\/span>\u5317\u4eac<\/span>. <\/span>\u6e05\u534e\u5927\u5b66\u51fa\u7248\u793e<\/span>. 2018.<\/span><\/span><\/p>\n

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\u8bc6\u522bqq\u7fa4\u4e8c\u7ef4\u7801\uff0c\u8bf7\u6ce8\u660e\uff1a\u201c\u516c\u53f8+\u7814\u7a76\u65b9\u5411\u201d\uff0c<\/strong>\u7b26\u5408\u6761\u4ef6\u8005\u5373\u53ef\u52a0\u5165\u7ec6\u80de\u57f9\u517bqq\u7fa4\uff01<\/strong><\/p>\n

\"\u4ece\u5317\u4eac\u5230\u4e16\u754c:<\/p>\n

\"\u4ece\u5317\u4eac\u5230\u4e16\u754c:<\/strong><\/span><\/span><\/p>\n<\/p><\/div>\n","protected":false},"excerpt":{"rendered":"

\u56fe\u7247\u6765\u6e90\uff1ahttps:\/\/www.statnews.com  \u4f17\u6240\u5468\u77e5\uff0c\u4e2d\u56fd\u4ed3\u9f20\u5375\u5de2\uff08CHO\uff09\u7ec6\u80de\u662f […]<\/p>\n","protected":false},"author":1,"featured_media":104337,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/104336"}],"collection":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/comments?post=104336"}],"version-history":[{"count":1,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/104336\/revisions"}],"predecessor-version":[{"id":104347,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/104336\/revisions\/104347"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media\/104337"}],"wp:attachment":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media?parent=104336"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/categories?post=104336"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/tags?post=104336"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}