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14px;\">\u6574\u5408\u4f4d\u70b9\u662f\u5f71\u54cd\u8f6c\u5f55\u7387\u7684\u4e3b\u8981\u56e0\u7d20,\u8fd9\u4e00\u73b0\u8c61\u79f0\u4e3a\u4f4d\u7f6e\u6548\u5e94\u3002\u8d28\u7c92DNA\u5728\u5bbf\u4e3b\u7ec6\u80de\u57fa\u56e0\u7ec4\u4e2d\u968f\u673a\u63d2\u5165\uff0c\u6839\u636e\u6574\u5408\u4f4d\u70b9\uff0c\u8868\u8fbe\u91cf\u53ef\u80fd\u9ad8\u4e5f\u53ef\u80fd\u4f4e\uff0c\u751a\u81f3\u4e0d\u8868\u8fbe\uff0c\u800c\u91cd\u7ec4\u7684\u57fa\u56e0\u53ef\u80fd\u968f\u7740\u65f6\u95f4\u5931\u53bb\u8868\u8fbe\u80fd\u529b\u3002\u4e3a\u514b\u670d\u4f4d\u7f6e\u6548\u5e94\uff0c\u4eba\u4eec\u5df2\u7ecf\u5f00\u53d1\u51fa\u591a\u79cd\u7b56\u7565\u3002\u4f8b\u5982\uff0c\u5728\u8f7d\u4f53\u4e24\u4fa7\u91c7\u7528\u6297\u963b\u904f\u5143\u4ef6\uff08\u5982\u8868\u8fbe\u589e\u5f3a\u5e8f\u5217\u5143\u4ef6\uff09\u3001\u5c06\u8f7d\u4f53\u7279\u5f02\u5730\u6574\u5408\u5165\u5f00\u653e\u67d3\u8272\u4f53\u7684\u4f4d\u70b9\u6216\u8005\u4f7f\u7528\u6838\u8c03\u63a7\u56e0\u5b50\uff08\u6838\u57fa\u8d28\u9644\u7740\u533a\u6216\u652f\u67b6\u9644\u7740\u533a\uff09\u3002<\/span><\/p>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><span style=\"line-height: 150%;font-size: 14px;\">\u67d0\u4e9b\u60c5\u51b5\u4e0b\uff0c\u9700\u8981\u5728\u8c03\u63a7\u8bf1\u5bfc\u4e0b\u8868\u8fbe\u4ea7\u54c1\uff0c\u800c\u4e0d\u662f\u7a33\u5b9a\u8fde\u7eed\u5730\u8868\u8fbe\u3002\u8bf1\u5bfc\u8868\u8fbe\u7cfb\u7edf\u5305\u62ec\u70ed\u4f11\u514b\u8bf1\u5bfc\u8868\u8fbe\u3001\u91cd\u91d1\u5c5e\u8bf1\u5bfc\u8868\u8fbe\u3001\u7cd6\u76ae\u8d28\u6fc0\u7d20\u8bf1\u5bfc\u8868\u8fbe\u3002\u4f46\u56e0\u4e3a\u8fd9\u4e9b\u8bf1\u5bfc\u8868\u8fbe\u7cfb\u7edf\u4ea7\u751f\u7684\u6548\u5e94\u5f71\u54cd\u8303\u56f4\u5e7f\u3001\u672c\u5e95\u8868\u8fbe\u9ad8\uff0c\u7814\u7a76\u8005\u5f00\u53d1\u51faLac\/IPTG\u3001\u56db\u73af\u7d20\u3001\u94fe\u9709\u9633\u7d20\u7cfb\u7edf\u7b49\u3002<\/span><\/p>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><span style=\"line-height: 150%;font-size: 14px;\">\u5355\u514b\u9686\u6297\u4f53\u7684\u91cd\u94fe\u548c\u8f7b\u94fe\u9700\u8981\u6309\u7167\u6700\u4f73\u7684\u7406\u8bba\u914d\u6bd4\u6bd4\u4f8b\u8868\u8fbe\u3002\u4e3a\u6b64\uff0c\u91c7\u7528\u4e86\u4e0d\u540c\u7684\u8f7d\u4f53\u8bbe\u8ba1\u7b56\u7565\u3002\u6700\u5e38\u89c1\u7684\u662f\u4e3a\u91cd\u94fe\u3001\u8f7b\u94fe\u5404\u8bbe\u8ba1\u4e00\u4e2a\u8f7d\u4f53\uff0c \u5171\u540c\u8f6c\u67d3\u8fdb\u5165\u5bbf\u4e3b\u7ec6\u80de\u3002\u76f8\u5bf9\u8868\u8fbe\u91cf\u7684\u63a7\u5236\u662f\u901a\u8fc7\u8f6c\u67d3\u7ec6\u80de\u7684\u8f7d\u4f53\u91cf\u6765\u8fdb\u884c\u7684\u3002\u56e0\u4e3a\u6574\u5408\u4f4d\u70b9\u7684\u4f4d\u7f6e\u6548\u5e94\u548c\u7ec6\u80de\u4e4b\u95f4\u8f6c\u67d3\u6570\u91cf\u7684\u4e0d\u5747\u8861\uff0c\u8fd9\u79cd\u63a7\u5236\u901a\u5e38\u662f\u6700\u4e0d\u51c6\u786e\u7684\u3002\u5c06\u4e0d\u540c\u57fa\u56e0\u653e\u5728\u540c\u4e00\u8f7d\u4f53\u4e2d\uff0c\u4f7f\u7528\u540c\u6837\u7684\u542f\u52a8\u5b50\uff0c\u53ef\u4ee5\u51c6\u786e\u63a7\u5236\u8868\u8fbe\u57fa\u56e0\u4e4b\u95f4\u7684\u6bd4\u4f8b\uff0c\u4f46\u8fd9\u4f1a\u5f15\u5165\u91cd\u7ec4\u4e8b\u4ef6\u7684\u98ce\u9669\u3002\u53e6\u4e00\u79cd\u7b56\u7565\u662f\u91c7\u7528\u540c\u4e00\u8f7d\u4f53\uff0c\u91cd\u94fe\u3001\u8f7b\u94fe\u5404\u81ea\u4f7f\u7528\u4e0d\u540c\u7684\u542f\u52a8\u5b50\u3002<\/span><\/p>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><br  \/><\/p>\n<h3 style=\"text-indent: 24px;line-height: 150%;text-align: justify;font-weight: bold;\"><span style=\"font-size: 14px;\"><strong><span style=\"line-height: 150%;\"><\/span><\/strong><\/span><\/h3>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"border-width: 0px;border-style: initial;border-color: rgb(239, 112, 96);overflow: hidden;box-sizing: border-box;\">\n<section style=\"display: inline-block;font-size: 1em;font-weight: inherit;text-align: inherit;text-decoration: inherit;color: rgb(255, 255, 255);border-color: rgb(239, 112, 96);box-sizing: border-box;\">\n<section class=\"135brush\"   style=\"border-left-width: 8px;border-left-style: solid;border-color: rgb(212, 43, 21);display: inline-block;line-height: 1.4em;padding: 5px 10px;height: 32px;vertical-align: top;font-weight: bold;float: left;color: inherit;box-sizing: border-box !important;background: rgb(239, 112, 96);\">3.2 \u8f6c\u67d3\u65b9\u6cd5<\/section>\n<section style=\"width: 0.5em;display: inline-block;height: 32px;vertical-align: top;border-bottom-width: 1em;border-bottom-style: solid;border-bottom-color: rgb(239, 112, 96);border-top-width: 1em;border-top-style: solid;border-top-color: rgb(239, 112, 96);border-left-color: rgb(239, 112, 96);color: inherit;box-sizing: border-box !important;border-right-width: 1em !important;border-right-style: solid !important;border-right-color: transparent !important;\"><\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><span style=\"line-height: 150%;font-size: 14px;\">\u53ef\u4ee5\u6839\u636e\u5e94\u7528\u65b9\u5f0f\u3001\u7ecf\u6d4e\u7684\u548c\u6280\u672f\u7684\u56e0\u7d20\uff0c\u9009\u62e9\u4f7f\u7528\u4e0d\u540c\u7684\u8f6c\u67d3\u65b9\u6cd5\u548c\u8f6c\u67d3\u7cfb\u7edf\uff0c\u9009\u62e9\u7a33\u5b9a\u8f6c\u67d3\u6216\u8005\u77ac\u65f6\u8f6c\u67d3\u3002<\/span><\/p>\n<h4 style=\"text-indent: 24px;line-height: 150%;text-align: justify;font-weight: bold;\"><span style=\"font-size: 14px;\"><strong><span style=\"line-height: 150%;\"><\/span><\/strong><\/span><\/h4>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"max-width: 100%;margin-top: 0.8em;margin-bottom: 0.5em;overflow: hidden;\">\n<section class=\"135brush\"  placeholder=\"\u8bf7\u8f93\u5165\u6807\u9898\" style=\"height: 36px;display: inline-block;color: rgb(255, 255, 255);font-weight: bold;padding-right: 10px;padding-left: 10px;line-height: 36px;vertical-align: top;box-sizing: border-box !important;background-color: rgb(239, 112, 96);\">3.2.1 \u7a33\u8f6c\u4e0e\u77ac\u8f6c<\/section>\n<section style=\"display: inline-block;height: 36px;vertical-align: top;border-left-width: 9px;border-left-style: solid;border-left-color: rgb(239, 112, 96);box-sizing: border-box !important;border-top-width: 18px !important;border-top-style: solid !important;border-top-color: transparent !important;border-bottom-width: 18px !important;border-bottom-style: solid !important;border-bottom-color: transparent !important;\"><\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><span style=\"line-height: 150%;font-size: 14px;\">\u751f\u4ea7\u4e34\u5e8a\u6216\u5546\u54c1\u5316\u5355\u6297\u901a\u5e38\u4f7f\u7528\u7a33\u8f6c\u7684\u65b9\u6cd5\uff0c\u83b7\u5f97\u957f\u671f\u8fde\u7eed\u7684\u8868\u8fbe\u3002\u7a33\u8f6c\u901a\u5e38\u52a0\u5165\u6807\u8bb0\u57fa\u56e0\u4ee5\u786e\u5b9a\u5916\u6e90\u57fa\u56e0\u6574\u5408\u5165\u5bbf\u4e3b\u7ec6\u80de\u67d3\u8272\u4f53\u3002\u7a33\u8f6c\u9700\u8981\u5927\u91cf\u4eba\u529b\u52b3\u52a8\u3001\u65f6\u95f4\u548c\u4eea\u5668\/\u8d44\u6e90\u7684\u6295\u5165\uff0c\u56e0\u6b64\u9700\u8981\u66f4\u7ecf\u6d4e\u5feb\u6377\u7684\u65b9\u5f0f\uff0c\u5982\u77ac\u8f6c\u3002\u77ac\u8f6c\u4e0e\u7a33\u8f6c\u8fc7\u7a0b\u76f8\u4f3c\uff0c\u533a\u522b\u662f\u4e0d\u9700\u8981\u90a3\u4e9b\u7528\u6765\u9274\u5b9a\u548c\u9009\u62e9\u5728\u8f6c\u67d3\u8fc7\u7a0b\u4e2d\u8d28\u7c92\u8f6c\u5165\u57fa\u56e0\u7ec4\u7684\u7ec6\u80de\u7684\u6b65\u9aa4\u3002\u4f46\u77ac\u8f6c\u7684\u65b9\u5f0f\u5916\u6e90DNA\u5728\u67d3\u8272\u4f53\u5916\uff0c\u5176\u8868\u8fbe\u91cf\u5c11\uff0c\u8fd8\u4e0d\u9002\u7528\u4e8e\u5927\u91cf\u8868\u8fbe\uff0c\u4f46\u9002\u7528\u4e8e\u9ad8\u901a\u91cf\u7b5b\u9009\u8fc7\u7a0b\u3001\u4f53\u5185\u8bc4\u4f30\u548c\u65e9\u671f\u7684\u4ea7\u54c1\u5206\u6790\u3002<\/span><\/p>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><span style=\"line-height: 150%;font-size: 14px;\">\u6700\u5e38\u7528\u7684\u6807\u8bb0\u57fa\u56e0\u662f\u4e8c\u6c22\u53f6\u9178\u8fd8\u539f\u9176\uff08DHFR\uff09\u548c\u8c37\u6c28\u9170\u80fa\u5408\u6210\u9176\uff08GS\uff09\u3002DHFR\u8868\u8fbe\u7cfb\u7edf\u662f\u57fa\u4e8e\u7f16\u7801DHFR\u9176\u7684dhfr\u57fa\u56e0\uff0c\u901a\u5e38\u7528\u4e8eCHO\u7ec6\u80de\u3002DHFR\u9176\u53c2\u4e0e\u6838\u82f7\u9178\u4ee3\u8c22\uff0c\u50ac\u5316\u4e8c\u6c22\u53f6\u9178 \u8f6c\u5316\u56db\u6c22\u53f6\u9178\u3002\u8f6c\u67d3\u6210\u529f\u7684\u7ec6\u80de\u83b7\u5f97\u4e86\u5bf9\u9057\u4f20\u9709\u7d20\uff08geneticin\uff09\u7684\u6297\u6027\u3002\u514b\u9686\u7b5b\u9009\u5728\u7f3a\u4e4fhypoxanthine \u548cthymidine\uff08H\/T\uff09\u548c\u542b\u9057\u4f20\u9709\u7d20\u7684\u57f9\u517b\u57fa\u4e2d\u8fdb\u884c\u3002\u53e6\u5916\uff0c\u8be5\u7b5b\u9009\u7cfb\u7edf\u8fd8\u53ef\u4ee5\u901a\u8fc7\u589e\u52a0MTX\uff08\u4e00\u79cdDHFR\u6291\u5236\u5242\uff09\u63d0\u9ad8\u9009\u62e9\u538b\u529b\uff0c\u7b5b\u9009\u83b7\u5f97\u76ee\u7684\u57fa\u56e0\u6269\u589e\u7684\u514b\u9686\u3002GS\u7b5b\u9009\u7cfb\u7edf\u7684\u539f\u7406\u662f\u5229\u7528\u8c37\u6c28\u9170\u80fa\u4ee3\u8c22\uff1a\u5bf9\u4e8e\u672c\u8eab\u7f3a\u4e4fGS\u7684\u7ec6\u80de\u7cfb\uff0c\u53ef\u4ee5\u901a\u8fc7GS\u6807\u8bb0\uff0c\u5728\u7f3a\u4e4f\u8c37\u6c28\u9170\u80fa\u7684\u672c\u8eab\u57f9\u517b\u57fa\u4e2d\u8fdb\u884c\u9009\u62e9\u7b5b\u9009\uff1b\u800c\u5bf9\u4e8e\u672c\u8eab\u80fd\u591f\u5408\u6210\u8db3\u591fG\u7684\u7ec6\u80de\u7cfb\uff0c\u53ef\u4ee5\u91c7\u7528\u7c7b\u4f3cDFHR\u7684\u65b9\u6cd5\uff0c\u52a0\u5165\u80fd\u6291\u5236\u5185\u6e90\u6027GS\u6d3b\u6027\u7684\u6291\u5236\u7269MTS\uff0c\u8fd9\u6837\u53ea\u6709\u90a3\u4e9b\u8f6c\u67d3\u4e86\u989d\u5916GS\u6d3b\u6027\u7684\u7ec6\u80de\u5f97\u4ee5\u751f\u5b58\u3002<\/span><\/p>\n<h4 style=\"text-indent: 24px;line-height: 150%;text-align: justify;font-weight: bold;\"><span style=\"font-size: 14px;\"><strong><span style=\"line-height: 150%;\"><\/span><\/strong><\/span><\/h4>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"max-width: 100%;margin-top: 0.8em;margin-bottom: 0.5em;overflow: hidden;\">\n<section class=\"135brush\"  placeholder=\"\u8bf7\u8f93\u5165\u6807\u9898\" style=\"height: 36px;display: inline-block;color: rgb(255, 255, 255);font-weight: bold;padding-right: 10px;padding-left: 10px;line-height: 36px;vertical-align: top;box-sizing: border-box !important;background-color: rgb(239, 112, 96);\">3.2.2.DNA \u4f20\u9001\u7cfb\u7edf<\/section>\n<section style=\"display: inline-block;height: 36px;vertical-align: top;border-left-width: 9px;border-left-style: solid;border-left-color: rgb(239, 112, 96);box-sizing: border-box !important;border-top-width: 18px !important;border-top-style: solid !important;border-top-color: transparent !important;border-bottom-width: 18px !important;border-bottom-style: solid !important;border-bottom-color: transparent !important;\"><\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><span style=\"line-height: 150%;font-size: 14px;\">DNA\u4f20\u9001\u7cfb\u7edf\u5305\u62ec\u78f7\u9178\u9499\u6c89\u6dc0\u3001\u7535\u8f6c\u3001\u8102\u8d28\u4f53\u8f6c\u67d3\u548c\u591a\u805a\u4f53\u8f6c\u67d3\u3002<\/span><\/p>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><span style=\"font-size: 14px;\"><span style=\"line-height: 150%;\">\u78f7\u9178\u9499\u6c89\u6dc0\u6700\u65e9\u88abGraham \u7b49\u57281973\u5e74\u63d0\u51fa\uff0c\u662f\u5e94\u7528\u6700\u5e7f\u6cdb\u7684\u65b9\u6cd5\u4e4b\u4e00\uff0c\u4e3b\u8981\u57fa\u4e8eDNA\u5f62\u6210\u6c89\u6dc0\u6216\u590d\u5408\u7269\u4ee5\u5167\u541e\u819c\u6ce1\u7684\u65b9\u5f0f\u8fdb\u5165\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\uff0c\u8be5\u65b9\u6cd5\u4ef7\u683c\u4f4e\u5ec9\u4e14\u5bf9\u591a\u79cd\u7ec6\u80de\u7c7b\u578b\u9002\u7528\u3002<\/span> <span style=\"line-height: 150%;\">\u7f3a\u70b9\u662f\u6548\u7387\u548c\u53ef\u91cd\u73b0\u4f4e\u3002\u5c3d\u7ba1\u6709\u4e00\u4e9b\u6539\u8fdb\u7b56\u7565,\u5982\u8f6c\u67d3\u540e\u52a0\u5165DMSO\u3001\u7518\u6cb9\u6216\u6c2f\u55b9\u5904\u7406\u589e\u52a0\u8868\u8fbe\u91cf\uff0c\u4f46\u8f6c\u67d3\u6548\u7387\u4ecd\u7136\u8f83\u4f4e\uff0c\u4e14\u8f6c\u67d3\u8fc7\u7a0b\u9700\u8981\u57f9\u517b\u57fa\u4e2d\u6709\u8840\u6e05\uff0c\u8f6c\u67d3\u7684\u7ed3\u679c\u975e\u5e38\u4f9d\u8d56\u4e8e\u64cd\u4f5c\u8fc7\u7a0b\u4e2d\u7684\u5fae\u5c0f\u53d8\u5316\u3002<\/span><\/span><\/p>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><span style=\"line-height: 150%;font-size: 14px;\">\u7535\u8f6c\u662f\u4e00\u79cd\u7b80\u5355\u5feb\u901f\u7684\u8f6c\u67d3\u65b9\u6cd5\uff0c\u901a\u8fc7\u8109\u51b2\u7535\u573a\u6270\u4e71\u7ec6\u80de\u8d28\u819c\u7684\u7535\u538b\u5dee\uff0c\u5f62\u6210\u53ef\u9006\u7684\u5c0f\u5b54\u5141\u8bb8DNA\u8fdb\u5165\u7ec6\u80de\u3002\u8fd0\u7528\u8be5\u65b9\u6cd5\u8f6c\u67d3\u540e\u7ec6\u80de\u7684\u751f\u5b58\u7387\u53d8\u4f4e\uff0c\u901a\u5e38\u7528\u4e8e\u9700\u8981\u5feb\u901f\u548c\u5c0f\u91cf\u751f\u4ea7\u7684\u5c0f\u89c4\u6a21\u5b9e\u9a8c\u3002<\/span><\/p>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><span style=\"line-height: 150%;font-size: 14px;\">\u8102\u8d28\u4f53\u8f6c\u67d3\u548c\u591a\u805a\u4f53\u8f6c\u67d3\u53ef\u80fd\u662f\u6700\u7b80\u5355\u7684\u53ef\u7528\u4e8e\u591a\u79cd\u7ec6\u80de\u7684\u65b9\u6cd5\u3002\u524d\u4e0e\u8005\u5728\u6709\u6216\u6ca1\u6709\u8840\u6e05\u7684\u60c5\u51b5\u4e0b\u90fd\u53ef\u4ee5\u4f7f\u7528\uff0c\u7c98\u9644\u7ec6\u80de\u6216\u60ac\u6d6e\u7ec6\u80de\u3002\u540e\u8005\u53ef\u7528\u4e8e\u65e0\u8840\u6e05\u7684\u60ac\u6d6e\u57f9\u517b\u7269\u79cd\u3002<\/span><\/p>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><br  \/><\/p>\n<h3 style=\"text-indent: 24px;line-height: 150%;text-align: justify;font-weight: bold;\"><span style=\"font-size: 14px;\"><strong><span style=\"line-height: 150%;\"><\/span><\/strong><\/span><\/h3>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"border-width: 0px;border-style: initial;border-color: rgb(239, 112, 96);overflow: hidden;box-sizing: border-box;\">\n<section style=\"display: inline-block;font-size: 1em;font-weight: inherit;text-align: inherit;text-decoration: inherit;color: rgb(255, 255, 255);border-color: rgb(239, 112, 96);box-sizing: border-box;\">\n<section class=\"135brush\"   style=\"border-left-width: 8px;border-left-style: solid;border-color: rgb(212, 43, 21);display: inline-block;line-height: 1.4em;padding: 5px 10px;height: 32px;vertical-align: top;font-weight: bold;float: left;color: inherit;box-sizing: border-box !important;background: rgb(239, 112, 96);\">3.3 \u9009\u62e9<\/section>\n<section style=\"width: 0.5em;display: inline-block;height: 32px;vertical-align: top;border-bottom-width: 1em;border-bottom-style: solid;border-bottom-color: rgb(239, 112, 96);border-top-width: 1em;border-top-style: solid;border-top-color: rgb(239, 112, 96);border-left-color: rgb(239, 112, 96);color: inherit;box-sizing: border-box !important;border-right-width: 1em !important;border-right-style: solid !important;border-right-color: transparent !important;\"><\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><span style=\"line-height: 150%;font-size: 14px;\">\u8f6c\u67d3\u540e\uff0c\u901a\u8fc7\u6807\u8bb0\u57fa\u56e0\u6765\u7b5b\u9009\u80fd\u4ea7\u751f\u76ee\u6807\u5355\u6297\u7684\u7ec6\u80de\uff0c\u8f6c\u5165\u7b2c\u4e8c\u4e2a\u57f9\u517b\u5bb9\u5668\u4e2d\uff0c\u653e\u5927\u57f9\u517b\u3002\u8fd9\u4e9b\u514b\u9686\u6839\u636e\u7ec6\u80de\u751f\u957f\u72b6\u51b5\u3001\u5355\u6297\u4ea7\u7269\u6d53\u5ea6\u3002\u9009\u51fa\u7684\u7ec6\u80de\u8fdb\u884c\u4e0b\u4e00\u8f6e\u7684\u57f9\u517b\u548c\u5206\u6790\uff0c\u6765\u786e\u8ba4\u4ea7\u91cf\u6c34\u5e73\u662f\u5426\u5f97\u4ee5\u7ef4\u6301\u3002\u6700\u521d\u9009\u62e9\u7684\u7ec6\u80de\u514b\u9686\u7684\u4ea7\u91cf\u53ef\u80fd\u4e0d\u7406\u60f3\uff0c\u53ef\u4ee5\u901a\u8fc7\u589e\u52a0\u9009\u62e9\u538b\u529b\uff08MTX, MTS\uff09\u6765\u589e\u52a0\u62f7\u8d1d\u6570\uff0c\u8fdb\u800c\u63d0\u9ad8\u8868\u8fbe\u6c34\u5e73\u548c\u4ea7\u91cf\u3002<\/span><\/p>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><span style=\"line-height: 150%;font-size: 14px;\">\u9664\u4e86\u86cb\u767d\u6d4b\u5b9a\u6d53\u5ea6\u5916\uff0c\u5728\u65e0\u8840\u6e05\u57f9\u517b\u57fa\u4e2d\u5f62\u6210\u514b\u9686\u7684\u80fd\u529b\u3001\u6297\u51cb\u4ea1\u80fd\u529b\u3001\u7ec6\u80de\u7279\u5f81\uff08\u7ec6\u80de\u5927\u5c0f\u88ab\u8ba4\u4e3a\u662f\u4ea7\u91cf\u7684\u4e3b\u8981\u7ec6\u80de\u5b66\u5f71\u54cd\u56e0\u7d20\uff09\u3001\u9002\u5408\u7684\u751f\u4ea7\u52a8\u529b\u5b66\u3001\u4ea7\u7269\u5f62\u6210\u7684\u7a33\u5b9a\u6027\u3001\u5728\u538b\u529b\u548c\u526a\u5207\u529b\u6761\u4ef6\u4e0b\u7ec6\u80de\u7684\u5065\u58ee\u6027\uff0c\u7279\u522b\u662f\u751f\u4ea7\u8fc7\u7a0b\u4e2d\u7a33\u5b9a\u5730\u4ea7\u751f\u5355\u6297\u7684\u80fd\u529b\uff0c\u8fd9\u4e9b\u56e0\u7d20\u5bf9\u751f\u4ea7\u8fc7\u7a0b\u975e\u5e38\u91cd\u8981\uff0c\u4e5f\u5e94\u5f53\u4e5f\u5305\u62ec\u5728\u9009\u62e9\u8fc7\u7a0b\u4e2d\u3002\u4e00\u822c\u8981\u6c42\u5de5\u4f5c\u7ec6\u80de\u5e93\u5efa\u5e93\u540e\u81f3\u5c1160\u4ee3\u7684\u751f\u4ea7\u6c34\u5e73\u662f\u7a33\u5b9a\u7684\u3002\u8fd9\u5728\u9009\u62e9\u538b\u529b\u4e0b\u662f\u901a\u5e38\u7a33\u5b9a\u7684\uff0c\u4f46\u6ca1\u6709\u9009\u62e9\u538b\u529b\u7684\u60c5\u51b5\u4e0b\uff0c\u5c31\u6709\u4e0d\u7a33\u5b9a\u7684\u8ff9\u8c61\u3002\u800c\u9009\u62e9\u538b\u529b\u7684\u6bd2\u6027\u4f1a\u5bfc\u81f4\u9ad8\u6210\u672c\u3001\u590d\u6742\u7684\u4e0b\u6e38\u7eaf\u5316\u5de5\u827a\u3002\u56e0\u6b64\u5728\u4e0d\u4f7f\u7528\u8fd9\u4e9b\u9009\u62e9\u6709\u6bd2\u7269\u8d28\u7684\u540c\u65f6\u4fdd\u8bc1\u7a33\u5b9a\u6027\u53d8\u5f97\u975e\u5e38\u91cd\u8981\u3002\u5229\u7528FHIS\u6280\u672f\u6765\u7814\u7a76\u6574\u5408\u4f4d\u70b9\u662f\u5426\u5c5e\u4e8e\u5df2\u77e5\u7684\u5f71\u54cd\u4ea7\u54c1\u7a33\u5b9a\u6027\u7684\u4f4d\u70b9\uff0c\u53ef\u80fd\u975e\u5e38\u6709\u7528\u3002\u8fd9\u6837\u53ef\u4ee5\u6392\u9664\u90a3\u4e9b\u4e0d\u7a33\u5b9a\u8868\u8fbe\u7684\u7ec6\u80de\uff0c \u4e0d\u9700\u8981\u6d6a\u8d39\u65f6\u95f4\u5bf9\u8fd9\u4e9b\u7ec6\u80de\u8fdb\u884c\u5f88\u957f\u65f6\u95f4\u7684\u4f20\u4ee3\u7a33\u5b9a\u6027\u5b9e\u9a8c\u3002<\/span><\/p>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><span style=\"line-height: 150%;font-size: 14px;\">FACS\u88ab\u7528\u4e8e\u57fa\u4e8e\u7ec6\u80de\u7279\u5f81\u7684\u5feb\u901f\u7b5b\u9009\uff0c\u4f8b\u5982\uff0cBorth\u7b49\u5f00\u53d1\u7684\u5355\u7ec6\u80de\u5206\u6ccc\u5b9e\u9a8c\u53ef\u4ee5\u6d4b\u91cf\u5355\u4e2a\u7ec6\u80de\u7684\u5206\u6ccc\u80fd\u529b\uff0c\u7136\u540e\u4f7f\u7528\u6d41\u5f0f\u7ec6\u80de\u5206\u9009\u7ed3\u5408\u57f9\u517b\u7269\u6761\u4ef6\uff0c\u53ef\u4ee5\u9ad8\u6548\u5730\u9009\u51fa\u60f3\u8981\u7684\u7ec6\u80de\uff0c\u514d\u53bb\u5728\u7b5b\u9009\u8fc7\u7a0b\u4e2d\u8fdb\u884c\u751f\u957f\u76f8\u5173\u7684\u751f\u4ea7\u52a8\u529b\u5b66\u7814\u7a76\u3002\u591a\u53c2\u6570FACS\u7ed3\u5408\u53cc\u8272\u8367\u5149\u62a5\u544a\u57fa\u56e0\u7684\u65b9\u6cd5\u4e0d\u9700\u8981\u91cd\u590d\u7684\u51c6\u5907\u5de5\u4f5c\u548c\u7e41\u91cd\u7684\u5de5\u4f5c\u6d41\u7a0b\uff0c\u572812\u5468\u5185\uff0c\u7ecf\u8fc71\u8f6eFACS\uff0c\u5355\u6297\u4ea7\u91cf\u63d0\u9ad838\u500d\u3002<\/span><\/p>\n<h2 style=\"text-indent: 24px;line-height: 150%;text-align: justify;font-weight: bold;\"><span style=\"font-size: 14px;\"><strong><span style=\"line-height: 150%;\"><\/span><\/strong><\/span><\/h2>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section class=\"135brush\"   placeholder=\"\u6d45\u8272\u8fb9\u6846\u6807\u9898\" style=\"margin-top: 10px;margin-bottom: 10px;white-space: normal;padding: 10px;max-width: 100%;line-height: 25px;color: rgb(255, 255, 255);font-weight: bold;box-shadow: rgb(153, 153, 153) 1px 1px 2px;border-left-width: 25px;border-left-style: solid;border-color: rgb(208, 168, 228);box-sizing: border-box;background-color: rgb(166, 91, 203);\">4 \u7ec6\u80de\u5de5\u7a0b\u5b66<\/section>\n<\/section>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><span style=\"line-height: 150%;font-size: 14px;\">\u8fd1\u5e74\u6765\uff0c\u4eba\u4eec\u901a\u8fc7\u6297\u51cb\u4ea1\u3001\u4ee3\u8c22\u5de5\u7a0b\u3001\u4f4e\u6e29\u751f\u957f\u7ec6\u80de\u5de5\u7a0b\u3001\u5206\u5b50\u4f34\u4fa3\u5de5\u7a0b\u3001\u7ffb\u8bd1\u540e\u52a0\u5de5\u8fd9\u4e9b\u7b56\u7565\u6765\u63d0\u9ad8\u8868\u8fbe\u4ea7\u91cf\u3002<\/span><\/p>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><br  \/><\/p>\n<h3 style=\"text-indent: 24px;line-height: 150%;text-align: justify;font-weight: bold;\"><span style=\"font-size: 14px;\"><strong><span style=\"line-height: 150%;\"><\/span><\/strong><\/span><\/h3>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"border-width: 0px;border-style: initial;border-color: rgb(239, 112, 96);overflow: hidden;box-sizing: border-box;\">\n<section style=\"display: inline-block;font-size: 1em;font-weight: inherit;text-align: inherit;text-decoration: inherit;color: rgb(255, 255, 255);border-color: rgb(239, 112, 96);box-sizing: border-box;\">\n<section class=\"135brush\"   style=\"border-left-width: 8px;border-left-style: solid;border-color: rgb(212, 43, 21);display: inline-block;line-height: 1.4em;padding: 5px 10px;height: 32px;vertical-align: top;font-weight: bold;float: left;color: inherit;box-sizing: border-box !important;background: rgb(239, 112, 96);\">4.1 \u6297\u51cb\u4ea1<\/section>\n<section style=\"width: 0.5em;display: inline-block;height: 32px;vertical-align: top;border-bottom-width: 1em;border-bottom-style: solid;border-bottom-color: rgb(239, 112, 96);border-top-width: 1em;border-top-style: solid;border-top-color: rgb(239, 112, 96);border-left-color: rgb(239, 112, 96);color: inherit;box-sizing: border-box !important;border-right-width: 1em !important;border-right-style: solid !important;border-right-color: transparent !important;\"><\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><span style=\"line-height: 150%;font-size: 14px;\">\u7531\u4e8e\u7ec6\u80de\u57f9\u517b\u5bf9\u73af\u5883\u654f\u611f\uff0c\u800c\u538b\u529b\u72b6\u6001\uff0c\u5982\u8425\u517b\u7f3a\u4e4f\u3001\u505c\u6b62\u63d0\u4f9b\u751f\u957f\u56e0\u5b50\u3001\u6c27\u6c14\u6709\u9650\u3001\u6bd2\u6027\u4ee3\u8c22\u7269\u79ef\u7d2f\u3001\u6e17\u900f\u538b\u4e0b\u964d\u3001\u526a\u5207\u529b\u6c34\u5e73\u8fc7\u9ad8\uff0c\u4f1a\u8bf1\u5bfc\u7ec6\u80de\u51cb\u4ea1\uff0c\u6700\u7ec8\u5f71\u54cd\u76ee\u6807\u86cb\u767d\u7684\u4ea7\u91cf\u3002<\/span><\/p>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><span style=\"line-height: 150%;font-size: 14px;\">\u975e\u9057\u4f20\u65b9\u6cd5\u662f\u6297\u51cb\u4ea1\u6700\u5bb9\u6613\u5b9e\u73b0\u7684\u65b9\u6cd5\uff0c\u5305\u62ec\u589e\u52a0\u5468\u671f\u6027\u8865\u5145\u8425\u517b\u7269\u8d28\u3001\u4f7f\u7528\u534a\u4e73\u7cd6\u800c\u4e0d\u662f\u8461\u8404\u7cd6 \u4f5c\u4e3a\u78b3\u6e90\u3001\u4f7f\u7528\u6838\u82f7\u9178\uff0c\u5982\u817a\u82f7\u9178\u3002\u5176\u4ed6\u7b56\u7565\u5305\u62ec\u6297\u51cb\u4ea1\u57fa\u56e0\u7684\u8fc7\u8868\u8fbe\u3002\u5df2\u7ecf\u6d4b\u8bd5\u7684\u6297\u51cb\u4ea1\u57fa\u56e0\u6709Bcl-2\uff0c Bcl-xL\uff0c30Kc6\uff0c Aven\uff0c XIAP, CrmA\u548cE1b-19K,\u8fd9\u4e9b\u57fa\u56e0\u540c\u65f6\u8fd8\u53ef\u4ee5\u63d0\u9ad8\u7ec6\u80de\u5bc6\u5ea6\u548c\u86cb\u767d\u6d4b\u5b9a\u6d53\u5ea6\u3002\u6b64\u5916\uff0c\u53ef\u4ee5\u4f7f\u7528\u6297\u51cb\u4ea1\u7684\u5316\u5b66\u7269\u8d28suramin\uff0cN-actylcsteine \uff08NAC\uff09\u7b49\u3002caspase-3\u5728\u7ec6\u80de\u51cb\u4ea1\u4e2d\u8d77\u7740\u91cd\u8981\u4f5c\u7528\uff0c\u901a\u8fc7\u53cd\u4e49\u6280\u672f\u4e0b\u8c03\u8be5\u86cb\u767d\u7684\u8868\u8fbe\u53ef\u7528\u4e8e\u6297\u51cb\u4ea1\u3002\u4f46\u8fd9\u79cd\u6548\u5e94\u53ea\u662f\u4e00\u65f6\u7684\uff0c\u56e0\u4e3a\u5f53\u4e00\u4e2a\u6548\u5e94\u56e0\u5b50\u88ab\u4e0b\u8c03\u65f6\uff0c\u7ec6\u80de\u503e\u5411\u4e8e\u4ee3\u507f\u6027\u4e0a\u8c03\u8868\u8fbe\u5176\u4ed6\u6548\u5e94\u56e0\u5b50\u3002\u53e6\u4e00\u79cd\u7b56\u7565\u662f\u901a\u8fc7\u6291\u5236\u7ec6\u80de\u5468\u671f\u8fdb\u5c55\uff0c\u8fdb\u800c\u964d\u4f4e\u7ec6\u80de\u6b7b\u4ea1\uff0c\u589e\u52a0\u7ec6\u80de\u6d3b\u7387\u548c\u86cb\u767d\u8868\u8fbe\u91cf\u3002<\/span><\/p>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><br  \/><\/p>\n<h3 style=\"text-indent: 24px;line-height: 150%;text-align: justify;font-weight: bold;\"><span style=\"font-size: 14px;\"><strong><span style=\"line-height: 150%;\"><\/span><\/strong><\/span><\/h3>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"border-width: 0px;border-style: initial;border-color: rgb(239, 112, 96);overflow: hidden;box-sizing: border-box;\">\n<section style=\"display: inline-block;font-size: 1em;font-weight: inherit;text-align: inherit;text-decoration: inherit;color: rgb(255, 255, 255);border-color: rgb(239, 112, 96);box-sizing: border-box;\">\n<section class=\"135brush\"   style=\"border-left-width: 8px;border-left-style: solid;border-color: rgb(212, 43, 21);display: inline-block;line-height: 1.4em;padding: 5px 10px;height: 32px;vertical-align: top;font-weight: bold;float: left;color: inherit;box-sizing: border-box !important;background: rgb(239, 112, 96);\">4.2 \u4ee3\u8c22\u5de5\u7a0b\u5b66<\/section>\n<section style=\"width: 0.5em;display: inline-block;height: 32px;vertical-align: top;border-bottom-width: 1em;border-bottom-style: solid;border-bottom-color: rgb(239, 112, 96);border-top-width: 1em;border-top-style: solid;border-top-color: rgb(239, 112, 96);border-left-color: rgb(239, 112, 96);color: inherit;box-sizing: border-box !important;border-right-width: 1em !important;border-right-style: solid !important;border-right-color: transparent !important;\"><\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><span style=\"line-height: 150%;font-size: 14px;\">\u4ee3\u8c22\u5de5\u7a0b\u5b66\u662f\u53e6\u4e00\u4e2a\u9057\u4f20\u64cd\u4f5c\u7684\u5173\u6ce8\u70b9\uff0c\u901a\u8fc7\u6291\u5236\u6bd2\u6027\u526f\u4ea7\u7269\u5982\u4e73\u9178\u548c\u94f5\u7684\u79ef\u7d2f\u6765\u95f4\u63a5\u63d0\u9ad8\u7ec6\u80de\u751f\u957f\u548c\u5355\u4f4d\u4f53\u79ef\u4ea7\u7387\u3002\u5e38\u7528\u4e24\u79cd\u9014\u5f84\uff1a\u63d0\u9ad8\u4e2d\u5fc3\u78b3\u4ee3\u8c22\u7684\u6548\u7387\u548c\u964d\u4f4e\u4e73\u7cd6\u79ef\u7d2f\u3002<\/span><\/p>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><br  \/><\/p>\n<h3 style=\"text-indent: 24px;line-height: 150%;text-align: justify;font-weight: bold;\"><span style=\"font-size: 14px;\"><strong><span style=\"line-height: 150%;\"><\/span><\/strong><\/span><\/h3>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"border-width: 0px;border-style: initial;border-color: rgb(239, 112, 96);overflow: hidden;box-sizing: border-box;\">\n<section style=\"display: inline-block;font-size: 1em;font-weight: inherit;text-align: inherit;text-decoration: inherit;color: rgb(255, 255, 255);border-color: rgb(239, 112, 96);box-sizing: border-box;\">\n<section class=\"135brush\"   style=\"border-left-width: 8px;border-left-style: solid;border-color: rgb(212, 43, 21);display: inline-block;line-height: 1.4em;padding: 5px 10px;height: 32px;vertical-align: top;font-weight: bold;float: left;color: inherit;box-sizing: border-box !important;background: rgb(239, 112, 96);\">4.3 \u7ec6\u80de\u4f4e\u6e29\u751f\u957f\u7684\u5de5\u7a0b\u5b66<\/section>\n<section style=\"width: 0.5em;display: inline-block;height: 32px;vertical-align: top;border-bottom-width: 1em;border-bottom-style: solid;border-bottom-color: rgb(239, 112, 96);border-top-width: 1em;border-top-style: solid;border-top-color: rgb(239, 112, 96);border-left-color: rgb(239, 112, 96);color: inherit;box-sizing: border-box !important;border-right-width: 1em !important;border-right-style: solid !important;border-right-color: transparent !important;\"><\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><span style=\"font-size: 14px;\"><span style=\"line-height: 150%;\">27\u2103-32<\/span><span style=\"line-height: 150%;color: rgb(51, 51, 51);\">\u2103<\/span><span style=\"line-height: 150%;\">\u65f6\uff0c\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u5355\u4f4d\u751f\u957f\u901f\u7387\u8f83\u4f4e\uff0c\u4f46\u7ec6\u80de\u6d3b\u7387\u9ad8\uff0c\u5185\u6e90\u7ec6\u80de\u86cb\u767d\u6c61\u67d3\u5c11,\u4f4e\u6e29\u57f9\u517b\u6761\u4ef6\u4e5f\u4e0e\u7ec6\u80de\u5355\u4f4d\u751f\u4ea7\u529b\u6709\u5173\u3002\u4f46\u662f\uff0c\u4f4e\u6e29\u6761\u4ef6\u4e0b\uff0c\u5355\u4f4d\u4f53\u79ef\u4ea7\u7387\u5374\u5e76\u4e0d\u968f\u7740\u5355\u4f4d\u751f\u4ea7\u529b\u589e\u52a0\u800c\u589e\u52a0\u3002\u4e8b\u5b9e\u4e0a\uff0c\u7814\u7a76\u4e2d\u89c2\u5bdf\u5230\u7ec6\u80de\u5355\u4f4d\u751f\u957f\u7387\u4e0e\u7ec6\u80de\u5355\u4f4d\u751f\u4ea7\u529b\u5448\u53cd\u6bd4\u7684\u5173\u7cfb\u3002\u7814\u7a76\u8005\u4eec\u8bd5\u56fe\u901a\u8fc7\u7f13\u89e3\u4f4e\u6e29\u5bf9\u751f\u957f\u7684\u6291\u5236\u3001\u4e0b\u8c03\u4f4e\u6e29\u8bf1\u5bfc\u7684RNA\u7ed3\u5408\u86cb\u767d\u4ee5\u4fc3\u8fdb\u4f4e\u6e29\u4e0b\u7ec6\u80de\u7684\u751f\u957f,\u91c7\u7528\u8fd9\u4e9b\u65b9\u6cd5\uff0c\u5c3d\u7ba1\u7ec6\u80de\u751f\u957f\u5f97\u5230\u4e86\u63d0\u9ad8\uff0c\u4f46\u5355\u4f4d\u4f53\u79ef\u4ea7\u91cf\u6ca1\u6709\u63d0\u9ad8\u3002\u8fd8\u9700\u8981\u7ee7\u7eed\u7814\u7a76\u4f4e\u6e29\u4e0b\u7ec6\u80de\u751f\u957f\u7f13\u6162\u7684\u5206\u5b50\u673a\u5236\u4ee5\u4fbf\u8fdb\u884c\u5bbf\u4e3b\u7ec6\u80de\u5de5\u7a0b\u5b66\u7814\u7a76\u3002\u7136\u800c\uff0c\u9ad8\u4ea7\u91cf\u7684\u7ec6\u80de\u682a\u7531\u4e8e\u989d\u5916\u4ee3\u8c22\u8d1f\u62c5\uff0c\u751f\u957f\u76f8\u5bf9\u7f13\u6162\u3002\u53ef\u9009\u7684\u4e00\u4e2a\u7b56\u7565\u662f\uff0c\u5148\u5728\u7ec6\u80de\u751f\u957f\u9636\u6bb5\u91c7\u752837\u2103\u57f9\u517b\u4ee5\u83b7\u5f97\u9ad8\u7684\u7ec6\u80de\u751f\u957f\u901f\u7387\uff0c\u7136\u540e\u5728\u751f\u4ea7\u9636\u6bb5\u8f6c\u5165\u4f4e\u6e29\u57f9\u517b\u5f97\u5230\u9ad8\u7684\u5355\u6297\u751f\u4ea7\u529b\uff0c\u4ee5\u83b7\u5f97\u9ad8\u7684\u5355\u4f4d\u4f53\u79ef\u4ea7\u91cf\u3002<\/span><\/span><\/p>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><br  \/><\/p>\n<h3 style=\"text-indent: 24px;line-height: 150%;text-align: justify;font-weight: bold;\"><span style=\"font-size: 14px;\"><strong><span style=\"line-height: 150%;\"><\/span><\/strong><\/span><\/h3>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"border-width: 0px;border-style: initial;border-color: rgb(239, 112, 96);overflow: hidden;box-sizing: border-box;\">\n<section style=\"display: inline-block;font-size: 1em;font-weight: inherit;text-align: inherit;text-decoration: inherit;color: rgb(255, 255, 255);border-color: rgb(239, 112, 96);box-sizing: border-box;\">\n<section class=\"135brush\"   style=\"border-left-width: 8px;border-left-style: solid;border-color: rgb(212, 43, 21);display: inline-block;line-height: 1.4em;padding: 5px 10px;height: 32px;vertical-align: top;font-weight: bold;float: left;color: inherit;box-sizing: border-box !important;background: rgb(239, 112, 96);\">4.4 \u5206\u5b50\u4f34\u4fa3\u5de5\u7a0b<\/section>\n<section style=\"width: 0.5em;display: inline-block;height: 32px;vertical-align: top;border-bottom-width: 1em;border-bottom-style: solid;border-bottom-color: rgb(239, 112, 96);border-top-width: 1em;border-top-style: solid;border-top-color: rgb(239, 112, 96);border-left-color: rgb(239, 112, 96);color: inherit;box-sizing: border-box !important;border-right-width: 1em !important;border-right-style: solid !important;border-right-color: transparent !important;\"><\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><span style=\"line-height: 150%;font-size: 14px;\">\u5355\u6297\u662f\u5206\u6ccc\u578b\u86cb\u767d\uff0c\u8bb8\u591a\u7814\u7a76\u8868\u660e\uff0c\u4e0a\u8c03\u4e0e\u86cb\u767d\u5206\u6ccc\u6709\u5173\u7684\u5185\u8d28\u7f51\uff08ER\uff09\u86cb\u767d\u8868\u8fbe\u91cf\u4e0e\u86cb\u767d\u8d28\u9ad8\u6c34\u5e73\u751f\u4ea7\u6709\u5bc6\u5207\u5173\u7cfb\u3002\u6700\u5e38\u7528\u7684\u9014\u5f84\u662fER\u5206\u5b50\u4f34\u4fa3\u7684\u8fc7\u8868\u8fbe\uff0c\u4f46\u53ef\u80fd\u4ea7\u751f\u4e0d\u540c\u7684\u7ed3\u679c\u3002\u4f8b\u5982\uff0c\u86cb\u767d\u8d28\u4e8c\u786b\u5316\u7269\u5f02\u6784\u9176\u8fc7\u8868\u8fbe\u5728\u4e0d\u540c\u7684\u5b9e\u9a8c\u4e2d\u5bfc\u81f4\u5404\u4e0d\u76f8\u540c\u7684\u7ed3\u679c\uff1a\u4ea7\u91cf\u589e\u52a0\u3001\u51cf\u5c11\u751a\u81f3\u4e0d\u5f71\u54cd\u3002\u5176\u4ed6\u7684\u5206\u5b50\u4f34\u4fa3\u5305\u62ec\u91cd\u94fe\u7ed3\u5408\u86cb\u767d\uff08BiP\uff09\u3001ERp57\u3001GRP94\u3001\u5185\u8d28\u7f51\u7d20\u3002\u7531\u4e8e\u8c03\u63a7\u7cfb\u7edf\u590d\u6742\uff0c\u4ec5\u4ec5\u4f5c\u7528\u4e8e\u5206\u6ccc\u9014\u5f84\u7684\u4e00\u4e2a\u90e8\u4ef6\u4e5f\u8bb8\u5e76\u4e0d\u662f\u6700\u597d\u7684\u65b9\u5f0f,\u6309\u7167\u5177\u6709\u529f\u80fd\u610f\u4e49\u7684\u6bd4\u4f8b\u7684\u65b9\u5f0f\u4f5c\u7528\u4e8e\u591a\u4e2a\u5206\u5b50\u4f34\u4fa3\u3001\u5171\u5206\u5b50\u4f34\u4fa3\u3001holdase\u548c\u6298\u53e0\u9176\u53ef\u80fd\u662f\u66f4\u597d\u7684\u7b56\u7565\u3002<\/span><\/p>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><br  \/><\/p>\n<h3 style=\"text-indent: 24px;line-height: 150%;text-align: justify;font-weight: bold;\"><span style=\"font-size: 14px;\"><strong><span style=\"line-height: 150%;\"><\/span><\/strong><\/span><\/h3>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"border-width: 0px;border-style: initial;border-color: rgb(239, 112, 96);overflow: hidden;box-sizing: border-box;\">\n<section style=\"display: inline-block;font-size: 1em;font-weight: inherit;text-align: inherit;text-decoration: inherit;color: rgb(255, 255, 255);border-color: rgb(239, 112, 96);box-sizing: border-box;\">\n<section class=\"135brush\"   style=\"border-left-width: 8px;border-left-style: solid;border-color: rgb(212, 43, 21);display: inline-block;line-height: 1.4em;padding: 5px 10px;height: 32px;vertical-align: top;font-weight: bold;float: left;color: inherit;box-sizing: border-box !important;background: rgb(239, 112, 96);\">4.5 \u7ffb\u8bd1\u540e\u52a0\u5de5\u5de5\u7a0b\u5b66\uff08\u7cd6\u57fa\u5316\uff09<\/section>\n<section style=\"width: 0.5em;display: inline-block;height: 32px;vertical-align: top;border-bottom-width: 1em;border-bottom-style: solid;border-bottom-color: rgb(239, 112, 96);border-top-width: 1em;border-top-style: solid;border-top-color: rgb(239, 112, 96);border-left-color: rgb(239, 112, 96);color: inherit;box-sizing: border-box !important;border-right-width: 1em !important;border-right-style: solid !important;border-right-color: transparent !important;\"><\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><span style=\"font-size: 14px;line-height: 150%;\">\u7cd6\u57fa\u5316\u53d8\u5316\u53ef\u5f71\u54cd\u5355\u6297\u5728\u4f53\u5185\u7684\u6027\u8d28\u548c\u7a33\u5b9a\u6027\uff0c\u662f\u8d28\u91cf\u76f8\u5173\u7684\u5c5e\u6027\u4e4b\u4e00\u3002\u4e3a\u4e86\u4fdd\u8bc1\u4ea7\u54c1\u6b63\u786e\u7684\u7cd6\u57fa\u5316\u6c34\u5e73\uff0c\u63d0\u9ad8\u5355\u6297\u7684\u6548\u5e94\u529f\u80fd\uff08\u5982\u6297\u4f53\u4f9d\u8d56\u7684\u7ec6\u80de\u6bd2\u4f5c\u7528ADCC\u548c\u8865\u4f53\u4f9d\u8d56\u7684\u7ec6\u80de\u6bd2\u4f5c\u7528CDC\uff09\uff0c\u4eba\u4eec\u5f00\u5c55\u4e86\u8bb8\u591a\u8bb8\u591a\u7cd6\u57fa\u5316\u5de5\u7a0b\u7814\u7a76\uff0c\u4e3b\u8981\u901a\u8fc7\u4e24\u79cd\u9014\u5f84\uff1a\u7cd6\u8f6c\u79fb\u9176\u7684\u8fc7\u8868\u8fbe\u548c\u63d0\u9ad8\u805a\u7cd6\u8d28\u91cf\u3002\u524d\u8005\u6bd4\u5982\uff0c\u8fc7\u8868\u8fbe\u534a\u4e73\u7cd6\u57fa\u8f6c\u79fb\u9176\u3001\u553e\u6db2\u9178\u8f6c\u79fb\u9176\u3001N-\u4e59\u9170\u6c28\u57fa\u8461\u8404\u7cd6\u8f6c\u79fb\u9176\u3002\u540e\u8005\u7684\u4f8b\u5b50\u5305\u62ec\uff0c\u5c06alpha-2,6\u553e\u6db2\u9178\u5f15\u5165\u5230CHO \u548cBHK\u7ec6\u80de\u6765\u5e2e\u52a9\u63d0\u9ad8\u805a\u7cd6\u7684\u8d28\u91cf\u548c\u529f\u80fd\uff08\u8fd9\u4e24\u79cd\u7ec6\u80de\u7f3a\u4e4f\u76f8\u5e94\u7684\u7279\u5f02\u7684\u553e\u6db2\u9178\u8f6c\u79fb\u9176\uff09\u3002<\/span><\/p>\n<h2 style=\"text-indent: 24px;line-height: 150%;text-align: justify;font-weight: bold;\"><span style=\"font-size: 14px;\"><strong><span style=\"line-height: 150%;\"><\/span><\/strong><\/span><\/h2>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section class=\"135brush\"   placeholder=\"\u6d45\u8272\u8fb9\u6846\u6807\u9898\" style=\"margin-top: 10px;margin-bottom: 10px;white-space: normal;padding: 10px;max-width: 100%;line-height: 25px;color: rgb(255, 255, 255);font-weight: bold;box-shadow: rgb(153, 153, 153) 1px 1px 2px;border-left-width: 25px;border-left-style: solid;border-color: rgb(208, 168, 228);box-sizing: border-box;background-color: rgb(166, 91, 203);\">5. \u9002\u5e94\u65b0\u7684\u57f9\u517b\u73af\u5883<\/section>\n<\/section>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><span style=\"line-height: 150%;font-size: 14px;\">\u751f\u7269\u836f\u7269\u4ea7\u54c1\u7684\u5927\u89c4\u6a21\u751f\u4ea7\u5de5\u827a\u4e3a\u60ac\u6d6e\u548c\u65e0\u8840\u6e05\u57f9\u517b\u57fa\u6761\u4ef6\u3002\u56e0\u6b64\uff0c\u5f97\u5230\u751f\u4ea7\u5355\u6297\u7684\u7ec6\u80de\u540e\uff0c\u9700\u8981\u9002\u5e94\u8fd9\u4e9b\u65b0\u7684\u6761\u4ef6\u3002<\/span><\/p>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><br  \/><\/p>\n<h3 style=\"text-indent: 24px;line-height: 150%;text-align: justify;font-weight: bold;\"><span style=\"font-size: 14px;\"><strong><span style=\"line-height: 150%;\"><\/span><\/strong><\/span><\/h3>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"border-width: 0px;border-style: initial;border-color: rgb(239, 112, 96);overflow: hidden;box-sizing: border-box;\">\n<section style=\"display: inline-block;font-size: 1em;font-weight: inherit;text-align: inherit;text-decoration: inherit;color: rgb(255, 255, 255);border-color: rgb(239, 112, 96);box-sizing: border-box;\">\n<section class=\"135brush\"   style=\"border-left-width: 8px;border-left-style: solid;border-color: rgb(212, 43, 21);display: inline-block;line-height: 1.4em;padding: 5px 10px;height: 32px;vertical-align: top;font-weight: bold;float: left;color: inherit;box-sizing: border-box !important;background: rgb(239, 112, 96);\">5.1 &nbsp;\u60ac\u6d6e\u57f9\u517b<\/section>\n<section style=\"width: 0.5em;display: inline-block;height: 32px;vertical-align: top;border-bottom-width: 1em;border-bottom-style: solid;border-bottom-color: rgb(239, 112, 96);border-top-width: 1em;border-top-style: solid;border-top-color: rgb(239, 112, 96);border-left-color: rgb(239, 112, 96);color: inherit;box-sizing: border-box !important;border-right-width: 1em !important;border-right-style: solid !important;border-right-color: transparent !important;\"><\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><span style=\"line-height: 150%;font-size: 14px;\">&nbsp;\u60ac\u6d6e\u57f9\u517b\u662f\u4e3a\u4e86\u83b7\u5f97\u9ad8\u7684\u57f9\u517b\u5bc6\u5ea6\u548c\u4ea7\u54c1\u91cf\u3002\u7531\u4e8e\u5929\u7136\u72b6\u6001\u4e0b\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u662f\u9ecf\u9644\u751f\u957f\u7684\uff0c\u60ac\u6d6e\u751f\u957f\u9700\u8981\u4e00\u4e2a\u9002\u5e94\u8fc7\u7a0b\u3002\u9002\u5e94\u8fc7\u7a0b\u4e2d\u6709\u51e0\u4e2a\u53c2\u6570\u6bd4\u8f83\u5173\u952e\uff0c\u5982\u521d\u59cb\u5bc6\u5ea6\u548c\u6d3b\u7387\u3002\u56e0\u4e3a\u5728\u9002\u5e94\u8fc7\u7a0b\u4e2d\u5f88\u9ad8\u6bd4\u4f8b\u7684\u7ec6\u80de\u65e0\u6cd5\u5b58\u6d3b\u4e0b\u6765\uff0c\u5c31\u9700\u8981\u9002\u5f53\u7684\u9ad8\u521d\u59cb\u5bc6\u5ea6,\u9700\u8981\u5728\u8fc7\u7a0b\u4e2d\u76d1\u63a7\u7ec6\u80de\u751f\u957f\u60c5\u51b5\u3001\u6d3b\u7387\u548c\u4ea7\u91cf\u3002\u901a\u5e38\u52a0\u5165\u7a33\u5b9a\u5242Fluronic F68\u4fdd\u62a4\u7ec6\u80de\uff0c\u51cf\u5c11\u526a\u5207\u529b\u5bf9\u7ec6\u80de\u7684\u4f24\u5bb3\u3002\u503c\u5f97\u6ce8\u610f\u7684\u662f\uff0c\u52a0\u5165\u4efb\u4f55\u7684\u6dfb\u52a0\u7269\u90fd\u53ef\u80fd\u5f71\u54cd\u4e0b\u6e38\u5de5\u827a\uff0c\u5e94\u8c28\u614e\u5206\u6790\u3002<\/span><\/p>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><br  \/><\/p>\n<h3 style=\"text-indent: 24px;line-height: 150%;text-align: justify;font-weight: bold;\"><span style=\"font-size: 14px;\"><strong><span style=\"line-height: 150%;\"><\/span><\/strong><\/span><\/h3>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"border-width: 0px;border-style: initial;border-color: rgb(239, 112, 96);overflow: hidden;box-sizing: border-box;\">\n<section style=\"display: inline-block;font-size: 1em;font-weight: inherit;text-align: inherit;text-decoration: inherit;color: rgb(255, 255, 255);border-color: rgb(239, 112, 96);box-sizing: border-box;\">\n<section class=\"135brush\"   style=\"border-left-width: 8px;border-left-style: solid;border-color: rgb(212, 43, 21);display: inline-block;line-height: 1.4em;padding: 5px 10px;height: 32px;vertical-align: top;font-weight: bold;float: left;color: inherit;box-sizing: border-box !important;background: rgb(239, 112, 96);\">5.2 &nbsp;\u65e0\u8840\u6e05\u57f9\u517b\u57fa<\/section>\n<section style=\"width: 0.5em;display: inline-block;height: 32px;vertical-align: top;border-bottom-width: 1em;border-bottom-style: solid;border-bottom-color: rgb(239, 112, 96);border-top-width: 1em;border-top-style: solid;border-top-color: rgb(239, 112, 96);border-left-color: rgb(239, 112, 96);color: inherit;box-sizing: border-box !important;border-right-width: 1em !important;border-right-style: solid !important;border-right-color: transparent !important;\"><\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><span style=\"line-height: 150%;font-size: 14px;\">\u8840\u6e05\u662f\u57f9\u517b\u57fa\u4e2d\u652f\u6301\u7ec6\u80de\u589e\u6b96\u91cd\u8981\u7684\u7ec4\u5206\u3002\u4f46\u8840\u6e05\u7528\u4e8e\u751f\u4ea7\u4eba\u7684\u6cbb\u7597\u836f\u7269\u6709\u8bb8\u591a\u7f3a\u70b9\uff1a1\uff09\u8840\u6e05\u4e2d\u5404\u7269\u8d28\u6d53\u5ea6\u7684\u6279\u95f4\u5dee\u5f02\u5f71\u54cd\u5de5\u827a\u4e00\u81f4\u6027\uff1b2\uff09\u7ed9\u4e0b\u6e38\u5de5\u827a\u5e26\u6765\u8d1f\u62c5\uff1b3\uff09\u9ad8\u6210\u672c\uff1b4\uff09\u4f20\u64ad\u75be\u75c5\u7684\u98ce\u9669\u3002\u56e0\u6b64\uff0c\u4f7f\u7528\u65e0\u8840\u6e05\u57f9\u517b\u57fa\u662f\u5546\u4e1a\u5316\u751f\u4ea7\u7684\u76ee\u6807\u4e4b\u4e00\u3002\u4e3a\u4e86\u5bfb\u627e\u80fd\u4ee3\u66ff\u8840\u6e05\u6ee1\u8db3\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u751f\u957f\u7684\u590d\u6742\u8425\u517b\u9700\u6c42\u7684\u7269\u8d28\uff0c\u4eba\u4eec\u8fdb\u884c\u4e86\u8bb8\u591a\u7684\u52aa\u529b\uff0c\u5305\u62ecHam\u2019s\u65b9\u6cd5\uff08\u9010\u6e10\u51cf\u5c11\u57f9\u517b\u57fa\u4e2d\u672a\u5b9a\u4e49\u7684\u6dfb\u52a0\u7269\uff09\u3001Sato\u65b9\u6cd5\uff08\u91cd\u5efa\u4e0e\u7ec6\u80de\u751f\u957f\u76f8\u5173\u7684\u80de\u5916\u73af\u5883\uff09\u7b49\uff0c\u8fd9\u4e9b\u65b9\u6cd5\u90fd\u6bd4\u8f83\u8017\u65f6\u3002\u7ec6\u80de\u5728\u65e0\u8840\u6e05\u57f9\u517b\u57fa\u4e2d\u7684\u9002\u5e94\u901a\u5e38\u901a\u8fc7\u4e24\u79cd\u9014\u5f84\u5b9e\u73b0\uff1a\u4e00\u6b65\u6216\u9010\u6e10\u964d\u4f4e\u57f9\u517b\u57fa\u4e2d\u7684\u8840\u6e05\u3002\u4e0d\u8bba\u54ea\u79cd\u65b9\u5f0f\uff0c\u7ec6\u80de\u751f\u957f\u7f13\u6162\u3001\u6d3b\u7387\u4f4e\u7684\u72b6\u6001\u90fd\u4f1a\u7ef4\u6301\u597d\u51e0\u4ee3\uff0c\u9700\u8981\u5b9a\u671f\u76d1\u63a7\u3002\u91c7\u7528\u9ad8\u7684\u63a5\u79cd\u5bc6\u5ea6\u3001\u66f4\u6362\u57f9\u517b\u57fa\u3001\u7ef4\u6301\u8db3\u591f\u7684\u79cd\u5b50\u5bc6\u5ea6\uff0c\u53ef\u4ee5\u4e00\u5b9a\u7a0b\u5ea6\u4e0a\u7f29\u77ed\u8fd9\u4e2a\u8fc7\u7a0b\u3002\u4e3a\u4f7f\u7ec6\u80de\u5bb9\u6613\u9002\u5e94\uff0c\u5e94\u51cf\u5c11\u9009\u62e9\u538b\u529b\uff0c\u4f46\u4e3a\u4e86\u4fdd\u6301\u57fa\u56e0\u7684\u9ad8\u8868\u8fbe\u6c34\u5e73,\u4ecd\u5e94\u8be5\u5728\u8fc7\u7a0b\u4e2d\u4fdd\u7559\u9009\u62e9\u538b\u529b\uff0c\u5c3d\u7ba1\u91c7\u7528\u5f88\u4f4e\u7684\u6d53\u5ea6\u3002<\/span><\/p>\n<h2 style=\"text-indent: 24px;line-height: 150%;text-align: justify;font-weight: bold;\"><span style=\"font-size: 14px;\"><strong><span style=\"line-height: 150%;\"><\/span><\/strong><\/span><\/h2>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section class=\"135brush\"   placeholder=\"\u6d45\u8272\u8fb9\u6846\u6807\u9898\" style=\"margin-top: 10px;margin-bottom: 10px;white-space: normal;padding: 10px;max-width: 100%;line-height: 25px;color: rgb(255, 255, 255);font-weight: bold;box-shadow: rgb(153, 153, 153) 1px 1px 2px;border-left-width: 25px;border-left-style: solid;border-color: rgb(208, 168, 228);box-sizing: border-box;background-color: rgb(166, 91, 203);\">6. \u7ed3\u8bed<\/section>\n<\/section>\n<p style=\"text-indent: 24px;line-height: 150%;text-align: justify;white-space: normal;\"><span style=\"font-size: 14px;line-height: 150%;\">\u8fd1\u5e74\u6765\uff0c\u5355\u6297\u5728\u6cbb\u7597\u5e94\u7528\u4e2d\u7684\u91cd\u8981\u6027\u65e5\u76ca\u589e\u52a0\u5f15\u8d77\u4e86\u5927\u89c4\u6a21\u751f\u4ea7\u6280\u672f\u548c\u7b56\u7565\u7684\u5feb\u901f\u53d1\u5c55\u3002\u4e3a\u4f18\u5316\u5355\u6297\u751f\u4ea7\u800c\u4ed8\u51fa\u7684\u52aa\u529b\u4ecd\u5728\u7ee7\u7eed\uff0c\u4f46\u901a\u5e38\u7684\u5173\u6ce8\u7126\u70b9\u5728\u4e8e\u53cd\u5e94\u5668\u8bbe\u8ba1\u548c\/\u6216\u8fd0\u884c\u3002\u7136\u800c\uff0c\u5728\u53cd\u5e94\u5668\u4e2d\u57f9\u517b\u4e4b\u524d\u7684\u6b65\u9aa4\uff0c\u6bd4\u5982\u8f6c\u67d3\u76ee\u7684\u57fa\u56e0\u5230\u5bbf\u4e3b\u7ec6\u80de\u3001\u9009\u62e9\u5408\u9002\u7684\u514b\u9686\u4ee5\u53ca\u9002\u5e94\u4e0d\u540c\u7684\u57f9\u517b\u6761\u4ef6\uff0c\u5bf9\u540e\u671f\u8868\u8fbe\u6c34\u5e73\u6709\u5f88\u5927\u7684\u5f71\u54cd\u3002\u56e0\u6b64\uff0c\u4f18\u5316\u5de5\u4f5c\u5e94\u8be5\u4ece\u6784\u5efa\u5355\u6297\u751f\u4ea7\u7ec6\u80de\u682a\u7684\u65e9\u671f\u6b65\u9aa4\u5c31\u5f00\u59cb\u3002\u53e6\u5916\uff0c\u8fd9\u4e9b\u6b65\u9aa4\u975e\u5e38\u8017\u65f6\uff0c\u5bf9\u8ffd\u6c42\u836f\u54c1\u4e0a\u5e02\u901f\u5ea6\u7684\u751f\u7269\u5236\u836f\u4f01\u4e1a\u6765\u8bf4\uff0c\u8fd9\u662f\u4e00\u4e2a\u4e3b\u8981\u7684\u7f3a\u70b9\u3002\u800c\u7ec6\u80de\u5de5\u7a0b\u5b66\u7684\u7814\u7a76\u5f88\u53ef\u80fd\u53ef\u4ee5\u5e2e\u52a9\u4eba\u4eec\u8de8\u8fc7\u8fd9\u4e2a\u969c\u788d\u3002\u5b83\u53ef\u80fd\u5b9e\u73b0\u7814\u7a76\u8005\u5bf9\u7ec6\u80de\u751f\u957f\u548c\u751f\u4ea7\u6548\u7387\u66f4\u597d\u7684\u63a7\u5236\u548c\u63d0\u9ad8\u7ec6\u80de\u5bf9\u65b0\u73af\u5883\u7684\u9002\u5e94\u529b\u3002\u4f46\u76ee\u524d\u4e3a\u6b62\uff0c\u67d0\u4e9b\u7ed3\u679c\u8fd8\u662f\u4e92\u76f8\u77db\u76fe\u7684\uff0c\u8fd9\u4e0e\u5bf9\u7ec6\u80de\u7684\u76f8\u5173\u673a\u7406\u7f3a\u4e4f\u8ba4\u8bc6\u6709\u5173\u3002\u5728\u6ca1\u6709\u83b7\u77e5\u66f4\u591a\u65b0\u7684\u8bc0\u7a8d\u77e5\u8bc6\u4e4b\u524d\uff0c\u6210\u529f\u5c06\u5f88\u5927\u7a0b\u5ea6\u4e0a\u4f9d\u8d56\u4e8e\u8bd5\u9519\u5b9e\u9a8c\uff0c\u7ec6\u80de\u5de5\u7a0b\u5b66\u7684\u8fdb\u5c55\u5c06\u4f9d\u8d56\u4e8e\u65b0\u7684\u53d1\u73b0\u53ca\u5176\u5e94\u7528\u3002<\/span><\/p>\n<p style=\"line-height: 150%;text-align: justify;white-space: normal;text-indent: 0em;\">\u53c2\u8003\u6587\u732e\uff1a<br  \/><\/p>\n<p style=\"text-align: left;\"><span style=\"font-size: 12px;\">Rita Costa A, Elisa Rodrigues M, Henriques M, Azeredo J, Oliveira R.Guidelines to cell engineering for monoclonal antibody production.<span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;font-size: 12px;line-height: 25.6px;\">Eur J Pharm Biopharm. 2010 Feb;74(2):127-38.&nbsp;<\/span><\/span><\/p>\n<hr style=\"border-style: solid;border-width: 1px 0 0;border-color: rgba(0,0,0,0.1);-webkit-transform-origin: 0 0;-webkit-transform: scale(1, 0.5);transform-origin: 0 0;transform: scale(1, 0.5);\"  \/>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(62, 62, 62);font-family: \u5fae\u8f6f\u96c5\u9ed1;font-size: 16px;white-space: normal;line-height: 24px;text-align: center;text-indent: 2em;box-sizing: border-box !important;word-wrap: break-word !important;background-color: rgb(255, 255, 255);\"><span style=\"max-width: 100%;text-indent: 2em;line-height: 25.6px;color: rgb(255, 41, 65);font-size: 20px;box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><\/strong><\/span><\/p>\n<p style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1;font-size: 16px;white-space: normal;max-width: 100%;min-height: 1em;letter-spacing: 0.544px;background-color: rgb(255, 255, 255);line-height: 32px;text-align: center;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 20px;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box;text-indent: 27px;letter-spacing: 0.54px;widows: 1;font-size: 18px;overflow-wrap: break-word !important;\"><span style=\"max-width: 100%;box-sizing: border-box;color: rgb(0, 209, 0);overflow-wrap: break-word 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