{"id":105552,"date":"2019-03-22T00:00:50","date_gmt":"2019-05-17T09:04:24","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/105552\/"},"modified":"2019-05-17T17:04:26","modified_gmt":"2019-05-17T09:04:26","slug":"09d7b131c8","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/105552\/","title":{"rendered":"\u5f20\u6797\u7426\/\u5411\u70e8\/\u5f20\u590d\u6625\u56e2\u961f\u63ed\u793a\u5be8\u5361\u75c5\u6bd2\u4eba\u6e90\u5355\u514b\u9686\u6297\u4f53\u7ed3\u6784\u548c\u4e2d\u548c\u4f5c\u7528\u673a"},"content":{"rendered":"
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2019\u5e743\u670819\u65e5\uff0c\u6e05\u534e\u5927\u5b66\u533b\u5b66\u9662<\/span>\u5f20\u6797\u7426\u3001\u5411\u70e8<\/strong><\/span>\u4e24\u4e2a\u8bfe\u9898\u7ec4\u4e0e\u5e7f\u5dde\u5e02\u7b2c\u516b\u4eba\u6c11\u533b\u9662<\/span>\u5f20\u590d\u6625<\/strong><\/span>\u79d1\u7814\u56e2\u961f\u5408\u4f5c\uff0c\u5728\u56fd\u9645\u671f\u520a<\/span> Reports<\/strong><\/span><\/em>\u5728\u7ebf\u53d1\u8868\u4e86\u9898\u4e3a<\/span>Structural basis for neutralization and protection by a Zika virus specific human antibody<\/strong><\/em><\/span>\u7684\u7814\u7a76\u8bba\u6587\u3002<\/span>\u8be5\u7814\u7a76\u7efc\u5408\u8fd0\u7528X-\u5c04\u7ebf\u6676\u4f53\u5b66\u4e0e\u51b7\u51bb\u7535\u955c\u4e09\u7ef4\u91cd\u6784\u6280\u672f\uff0c\u89e3\u6790\u4e86\u9ad8\u6548\u548c\u4fdd\u62a4\u6027\u6297\u4f53ZK2B10\u4e0e\u5be8\u5361\u75c5\u6bd2E\u86cb\u767d\u590d\u5408\u7269\u7684\u6676\u4f53\u7ed3\u6784\u548c\u4e0e\u6210\u719f\u5be8\u5361\u75c5\u6bd2\u9897\u7c92\u590d\u5408\u7269\u7684\u7535\u955c\u7ed3\u6784\uff0c\u4e3a\u6df1\u5165\u63a2\u7d22ZK2B10\u7684\u4e2d\u548c\u548c\u4fdd\u62a4\u673a\u5236\u63d0\u4f9b\u4e86\u5173\u952e\u7684\u7ed3\u6784\u57fa\u7840\u3002<\/strong><\/span><\/p>\n


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\u5728\u8fd9\u9879\u7814\u7a76\u4e2d\uff0c\u7814\u7a76\u4eba\u5458\u5408\u4f5c<\/span>\u89e3\u6790\u4e86ZK2B10\u4e0e\u5be8\u5361\u75c5\u6bd2E\u86cb\u767d\u590d\u5408\u7269\u7684\u6676\u4f53\u7ed3\u6784\u548c\u4e0e\u6210\u719f\u5be8\u5361\u75c5\u6bd2\u9897\u7c92\u590d\u5408\u7269\u7684\u7535\u955c\u7ed3\u6784\uff0c\u63a2\u7d22\u4e86\u4e2d\u548c\u548c\u4fdd\u62a4\u673a\u5236\u3002<\/strong><\/span>\u7814\u7a76\u53d1\u73b0\uff0cZK2B10\u8bc6\u522b\u7684\u8868\u4f4d\u4f4d\u4e8eE\u86cb\u767d\u7b2c\u4e09\u7ed3\u6784\u57df\u7684\u4fa7\u810a\uff08the lateralridge of DIII\uff09\uff1b\u76f8\u8f83\u5df2\u77e5\u7684\u9ec4\u75c5\u6bd2\u540c\u7c7b\u4e2d\u548c\u6297\u4f53\uff0cZK2B10\u7ed3\u5408\u6297\u539f\u7684\u89d2\u5ea6\u72ec\u7279\uff0c\u4f5c\u7528\u9762\u79ef\u7cbe\u5c0f\uff0c\u4e0d\u4ec5\u80fd\u591f\u7ed3\u5408\u4e09\u6b21\u8f74\u548c\u4e8c\u6b21\u8f74\u9644\u8fd1\u800c\u4e14\u80fd\u591f\u7ed3\u5408\u4e94\u6b21\u8f74\u9876\u70b9\u5904\u7684\u7b2c\u4e09\u7ed3\u6784\u57df\uff0c\u5c55\u793a\u5176\u7ed3\u5408\u4e43\u81f3\u4e2d\u548c\u5be8\u5361\u75c5\u6bd2\u7684\u72ec\u5230\u7279\u70b9\u3002<\/span><\/strong><\/p>\n


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\"\u5f20\u6797\u7426\/\u5411\u70e8\/\u5f20\u590d\u6625\u56e2\u961f\u63ed\u793a\u5be8\u5361\u75c5\u6bd2\u4eba\u6e90\u5355\u514b\u9686\u6297\u4f53\u7ed3\u6784\u548c\u4e2d\u548c\u4f5c\u7528\u673a\"<\/p>\n

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Highlights<\/span><\/strong><\/span><\/strong><\/span><\/strong><\/span><\/strong><\/span><\/p>\n

\u2022A potent and protective human ZIKV-specificantibody was characterized<\/span><\/p>\n

\u2022Crystal structure defined the epitope at aresolution of 2.32 \u00c5<\/span><\/p>\n

\u2022Cryo-EM analyses revealed the unique coverage onthe mature virion<\/span><\/p>\n

\u2022The antibody interferes with postattachment stepsof ZIKV infection<\/span><\/p>\n

 <\/p>\n

Summary<\/span><\/strong><\/span><\/strong><\/span><\/strong><\/span><\/strong><\/span><\/p>\n

We previously reported a human monoclonal antibody, ZK2B10, capable of protection against Zika virus (ZIKV) infection andmicrocephaly in developing mouse embryos. Here, we report the structural features and mechanism of action of ZK2B10. The crystal structure at are solution of 2.32 \u00c5 revealed that the epitope is located on the lateral ridge of DIII of the envelope glycoprotein. Cryo-EM structure with mature ZIKV showed that the antibody binds to DIIIs around the icosahedral 2-fold, 3-fold, and5-fold axes, a distinct feature compared to those reported for DIII-specific antibodies. The binding of ZK2B10 to ZIKV has no detectable effect on viral attachment to target cells or on conformational changes of the E glycoproteinin the acidic environment, suggesting that ZK2B10 functions at steps between the formation of the fusion intermediate and membrane fusion. These results provide structural and mechanistic insights into how ZK2B10 mediates protection against ZIKV infection.<\/span><\/p>\n


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\u539f\u6587\u94fe\u63a5\uff1a<\/span><\/strong><\/span><\/strong><\/span><\/p>\n

1.Lin Wang, Ruoke Wang, Lei Wang, Haijing Ben, Lei Yu,Fei Gao, Xuanling Shi, Chibiao Yin, Fuchun Zhang, Ye Xiang, Linqi Zhang. <\/span><\/span>Structural Basis for Neutralization and Protection by a Zika Virus-Specific Human Antibody<\/strong><\/em><\/span>. <\/span><\/span> Rep<\/strong><\/em><\/span>. 2019, 26(12): P3360-3368.e5, March 19, 2019<\/span><\/p>\n

DOI:https:\/\/doi.org\/10.1016\/j.celrep.2019.02.062<\/span><\/p>\n

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http:\/\/www.tsinghua.edu.cn\/publish\/thunews\/10303\/2019\/20190320092401085407147\/20190320092401085407147_.html<\/span>
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