{"id":105739,"date":"2019-03-25T00:00:50","date_gmt":"2019-05-17T09:05:45","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/105739\/"},"modified":"2019-05-17T17:05:48","modified_gmt":"2019-05-17T09:05:48","slug":"3e1cea6a71","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/105739\/","title":{"rendered":"\u4f4e\u6210\u672c\u3001\u5feb\u901f\u751f\u4ea7\u75ab\u82d7\u7684\u65b0\u5174\u6280\u672f"},"content":{"rendered":"<div class=\"bpp-post-content\">\n<section  label=\"Powered by 135editor.com\" style=\"font-size: 16px;\">\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"width: 100%;\" >\n<section style=\"text-align: right;\">\n<section style=\"width:3em;display: inline-block;\"><img class=\"\"      style=\"width: 100%;display: block;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/7-1558083946.png\" alt=\"\u4f4e\u6210\u672c\u3001\u5feb\u901f\u751f\u4ea7\u75ab\u82d7\u7684\u65b0\u5174\u6280\u672f\" title=\"\u4f4e\u6210\u672c\u3001\u5feb\u901f\u751f\u4ea7\u75ab\u82d7\u7684\u65b0\u5174\u6280\u672f\"  \/><\/section>\n<\/section>\n<section style=\"padding: 0em 1em;margin: -1.5em auto;box-sizing: border-box;\">\n<section  class=\"135brush\" style=\"border-width: 1px;border-style: solid;border-color: rgb(235, 235, 253);font-size: 14px;color: rgb(140, 139, 203);box-sizing: border-box;border-radius: 10px;letter-spacing: 1.5px;line-height: 1.75em;padding: 1.5em 1em;\"><strong><span style=\"font-family: \u5b8b\u4f53, SimSun;\">\u6458\u8981\uff1a<\/span><\/strong><span style=\"font-family: \u5b8b\u4f53, SimSun;\">\u7075\u6d3b\u3001\u5feb\u901f\u548c\u4f4e\u6210\u672c\u7684\u75ab\u82d7\u5f00\u53d1\u548c\u5236\u9020\u6280\u672f\u53ef\u4ee5\u963b\u6b62\u672a\u6765\u6d41\u884c\u75c5\u7684\u4f20\u64ad\u5e76\u6ee1\u8db3\u4f4e\u6536\u5165\u548c\u4e2d\u7b49\u6536\u5165\u56fd\u5bb6\u7684\u5a74\u513f\u75ab\u82d7\u63a5\u79cd\u9700\u6c42\u3002\u76ee\u524d\u80fd\u591f\u751f\u4ea7\u591a\u79cd\u75ab\u82d7\u7684\u75ab\u82d7\u5f00\u53d1\u5e73\u53f0\u6280\u672f\u6b63\u5728\u6d8c\u73b0\uff0c\u5176\u4e2d\u5305\u62ec: 1. \u4eba\u6e90\u5316\u7684\u3001\u9ad8\u4ea7\u91cf\u7684\u9175\u6bcd\u91cd\u7ec4\u86cb\u767d\u75ab\u82d7\uff1b2. \u6606\u866b\u7ec6\u80de-\u6746\u72b6\u75c5\u6bd2ADDomer\u75ab\u82d7\uff1b 3. \u819c\u6297\u539f\u75ab\u82d7\u901a\u7528\u6a21\u5757\uff08GMMA\uff09; 4. 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style=\"text-indent: 28px;line-height: 150%;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">\u8fd9\u5bf9\u4e8e\u4f4e\u6536\u5165\u56fd\u5bb6\u4ee5\u4f4e\u6210\u672c\u4e3a\u5a74\u513f\u548c\u5e7c\u513f\u63a5\u79cd\u75ab\u82d7\u63d0\u4f9b\u75ab\u82d7\uff0c\u4ee5\u53ca\u8fc5\u901f\u5e94\u5bf9\u533a\u57df\u66b4\u53d1\u4e2d\u7684\u65b0\u5a01\u80c1\uff0c\u59822014\u5e74\u897f\u975e\u57c3\u535a\u62c9\u75ab\u60c5\u548c2015-2016\u5e74\u5be8\u5361\u75ab\u60c5\uff0c\u5c24\u5176\u91cd\u8981\u3002\u4e3a\u4e86\u89e3\u51b3\u8fd9\u4e9b\u7d27\u8feb\u7684\u9700\u6c42\uff0c\u6b63\u5728\u5f00\u53d1\u65b0\u7684\u75ab\u82d7\u5e73\u53f0\u6280\u672f\uff0c\u5e94\u4f7f\u8fc5\u901f\u3001\u4f4e\u6210\u672c\u7684\u8fc7\u7a0b\u5f00\u53d1\u548c\u6269\u5927\u751f\u4ea7\u6210\u4e3a\u53ef\u80fd\uff0c\u4f8b\u5982\uff1a1. \u4eba\u6e90\u7684\u3001\u9ad8\u6536\u83b7\u7387\u9175\u6bcd\u5e73\u53f0\u7528\u4e8e\u91cd\u7ec4\u86cb\u767d\u75ab\u82d7\u751f\u4ea7\uff1b2. 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14px;font-family: \u5b8b\u4f53, SimSun;\">\u6b64\u5916\uff0c\u8fd9\u56db\u4e2a\u5e73\u53f0\u8fd8\u53ef\u4ee5\u8868\u73b0\u51fa\u81ea\u4f50\u5242\u6027\u3002ADDomer\u548cGMMA\u53ef\u4ee5\u901a\u8fc7\u7f16\u8f91\u5728\u5176\u8868\u9762\u66b4\u9732\u7c7b\u4f3c\u4f50\u5242\u7684\u5b9e\u4f53\uff1b\u4eba\u6e90\u5316\u7684\u3001\u9ad8\u4ea7\u91cf\u7684\u9175\u6bcd\u4ea7\u751f\u7684\u86cb\u767d\u5177\u6709\u4f50\u5242\u529f\u80fd\uff1bRNA\u5206\u5b50\u53ef\u4ee5\u5177\u6709\u81ea\u4f50\u5242\u6297\u539f\u80bd\u3002\u672c\u6587\u5bf9\u8fd9\u56db\u79cd\u65b0\u51fa\u73b0\u7684\u75ab\u82d7\u5e73\u53f0\u6280\u672f\u5728\u8f83\u4f4e\u7684\u6210\u672c\u5feb\u901f\u751f\u4ea7\u591a\u79cd\u75ab\u82d7\u65b9\u9762\u8fdb\u884c\u4e86\u6bd4\u8f83\u8bc4\u4ef7\u3002<\/span><\/p>\n<p style=\"line-height: 150%;\"><br  \/><\/p>\n<p><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><strong>\u88681 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style=\"display:inline-block;clear: both;\">\n<section style=\"padding: 6px 20px;background-image: url('http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/7-1558083948.jpeg');background-repeat: no-repeat;background-size: 100% 100%;letter-spacing: 1.5px;box-sizing: border-box;\">\n<section class=\"135brush\"  style=\"color: rgb(255, 255, 255);\">2. \u56db\u79cd\u75ab\u82d7\u5e73\u53f0\u6280\u672f\u6982\u8ff0<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 28px;line-height: 150%;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, 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SimSun;\">\u5bf9\u4e8e\u914d\u4f0d\uff0c\u6297\u539f\u53ef\u4ee5\u5438\u9644\u5230\u94dd\u5316\u5408\u7269\u4e0a\uff08\u5982\u6c22\u6c27\u5316\u94dd\uff09\u3002\u65b0\u578b\u4f50\u5242\uff0c\u5982toll\u6837\u53d7\u4f53\u6fc0\u52a8\u5242\uff08\u5355\u78f7\u9178\u8102\u8d28A\u548c\u514d\u75ab\u523a\u6fc0CpG-motif\u5be1\u6838\u82f7\u9178\uff09\u88ab\u8bc1\u660e\u80fd\u4f7f\u4fdd\u62a4\u66f4\u5feb\u5e76\u63d0\u9ad8\u6548\u679c\u3002\u75ab\u82d7\u4e2d\u4e5f\u6dfb\u52a0\u4e86\u9632\u8150\u5242\uff08\u5982\u786b\u67f3\u6c5e\uff09\uff0c\u4f46\u662f\uff0c\u8fd9\u4e9b\u9632\u8150\u5242\u5728\u5927\u591a\u6570\u73b0\u4ee3\u914d\u65b9\u4e2d\u5e76\u4e0d\u4f7f\u7528\uff0c\u56e0\u4e3a\u751f\u4ea7\u6280\u672f\u7684\u65e0\u83cc\u6027\u5f97\u5230\u4e86\u6539\u5584\uff0c\u4e0d\u518d\u9700\u8981\u9632\u8150\u5242\u3002\u75ab\u82d7\u53ef\u4ee5\u662f\u5355\u4ef7\u7684\uff08\u5305\u542b\u5355\u72ec\u6bd2\u682a\u7684\u5355\u4e2a\u6297\u539f\uff09\uff0c\u591a\u4ef7\u7684\uff08\u5305\u542b\u4e24\u79cd\u6216\u591a\u79cd\u6bd2\u682a\u3001\u8840\u6e05\u578b\u7684\u540c\u79cd\u6297\u539f\uff09\uff0c\u6216\u662f\u8054\u5408\u75ab\u82d7\uff08\u6df7\u5408\u5355\u4ef7\/\u591a\u4ef7\u75ab\u82d7\u9884\u9632\u591a\u79cd\u6216\u4e00\u79cd\u75be\u75c5\uff09\u3002\u75ab\u82d7\u901a\u5e38\u662f\u4ee5\u6db2\u4f53\u5f62\u5f0f\u5b58\u5728\u7684\uff0c\u7136\u800c\uff0c\u75ab\u82d7\u4e5f\u88ab\u51bb\u5e72\u4ee5\u589e\u52a0\u5176\u4fdd\u8d28\u671f\u3002\u7ecf\u914d\u4f0d\u540e\uff0c\u5e94\u5c06\u4e0b\u5217\u53c2\u6570\u63a7\u5236\u5728\u7684\u6700\u4f18\u8303\u56f4\u5185:\u6eb6\u6db2pH\u3001\u79bb\u5b50\u5f3a\u5ea6\u3001\u6c27\u5316\u8fd8\u539f\u7535\u4f4d\u3001\u6d53\u5ea6\u548c\u6e29\u5ea6\u3002<\/span><\/p>\n<p style=\"text-indent: 28px;line-height: 150%;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">\u5728\u8d28\u63a7\u65b9\u9762\uff0c\u4f7f\u7528\u6c28\u57fa\u9178\u6d4b\u5e8f\u3001\u86cb\u767d\u8d28\u5370\u8ff9\u6cd5\u3001\u51dd\u80f6\u7535\u6cf3\u3001\u9ad8\u6548\u6db2\u76f8\u8272\u8c31\u6cd5\u5bf9\u75ab\u82d7\u6d3b\u6027\u6210\u5206\u8fdb\u884c\u7ed3\u6784\u8868\u5f81\uff08\u5982\uff0c\u6c28\u57fa\u9178\u7ec4\u6210\u3001\u90e8\u5206\u6c28\u57fa\u9178\u5e8f\u5217\u3001\u80bd\u56fe\u3001\u8102\u8d28\u548c\u78b3\u6c34\u5316\u5408\u7269\u7ed3\u6784\u3001\u6d6e\u529b\u5bc6\u5ea6\u3001\u8868\u4f4d\u8868\u5f81\uff09\u3002\u6b64\u5916\uff0c\u5728\u914d\u4f0d\u524d\u8fd8\u5e94\u5b9a\u671f\u68c0\u67e5\u5176\u6297\u539f\u6027\u548c\u65e0\u83cc\u6027\u3002\u5bf9\u4e8e\u914d\u4f0d\u540e\u7684\u75ab\u82d7\uff0c\u5e94\u68c0\u6d4b\u5176\u86cb\u767d\u8d28\u548c\u94dd\u7684\u542b\u91cf\u3001\u70ed\u539f\u6027\u548c\u4f53\u5185\u6d3b\u6027(\u6d4b\u5b9a\u65b9\u6cd5\u4e3aED<sub>50<\/sub>)\u3002\u6b64\u5916\uff0c\u4e3a\u4e86\u8d28\u91cf\u63a7\u5236\uff0c\u5728\u6574\u4e2a\u751f\u4ea7\u8fc7\u7a0b\u4e2d\uff0c\u4ee5\u4e0b\u53c2\u6570\u7684\u6d4b\u91cf\u5e94\u4fdd\u6301\u5728\u6700\u4f73\u8303\u56f4\u5185:\u6e29\u5ea6\u3001\u538b\u529b\u3001pH\u3001\u7535\u5bfc\u3001\u5404\u79cd\u6210\u5206\u7684\u6d53\u5ea6\u3001\u5747\u4e00\u6027\u3001\u5316\u5b66\u548c\u751f\u7269\u6c61\u67d3\u7b49\u3002<\/span><\/p>\n<p style=\"text-indent: 28px;line-height: 150%;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">\u914d\u4f0d\u540e\u901a\u8fc7\u4e86\u8d28\u63a7\u7684\u4ea7\u54c1\uff0c\u5c06\u88ab\u7f50\u88c5\u8fdb\u65e0\u83cc\u7684\u73bb\u7483\u74f6\u3001\u5851\u6599\u897f\u6797\u74f6\u3001\u73bb\u7483\u7ba1\u3001\u5851\u6599\u7ba1\u4e2d\u3002\u74f6\u5b50\u63a5\u4e0b\u6765\u4f1a\u88ab\u88c5\u4e0a\u76d6\u5b50\uff0c\u5bc6\u5c01\uff0c\u8d34\u6807\uff0c\u5305\u88c5\uff0c\u6700\u540e\u5206\u53d1\u51fa\u5382\u3002<\/span><\/p>\n<p style=\"text-align: center;\"><img class=\"rich_pages\"       style=\"\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/0-1558083947.jpg\" alt=\"\u4f4e\u6210\u672c\u3001\u5feb\u901f\u751f\u4ea7\u75ab\u82d7\u7684\u65b0\u5174\u6280\u672f\" title=\"\u4f4e\u6210\u672c\u3001\u5feb\u901f\u751f\u4ea7\u75ab\u82d7\u7684\u65b0\u5174\u6280\u672f\"  \/><\/p>\n<p style=\"font-size: 16px;white-space: normal;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><strong>A.&nbsp;<\/strong><strong>\u7ec6\u83cc\u819c\u6297\u539f\uff08<\/strong><strong>GMMA<\/strong><strong>\uff09\u75ab\u82d7\u4ea7\u54c1\u7684\u5927\u6982\u6d41\u7a0b<\/strong><\/span><\/p>\n<p style=\"font-size: 16px;white-space: normal;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><strong>\u4e0a\u6e38\u8fc7\u7a0b\uff1a\u7ec6\u83cc\u7ec6\u80de\u590d\u82cf<\/strong><strong>&#8211;<\/strong><strong>\u7ec6\u83cc\u57f9\u517b\uff08\u6447\u74f6\uff0c<\/strong><strong>30\u2103<\/strong><strong>\uff0c<\/strong><strong>220rpm<\/strong><strong>\uff09<\/strong><strong>&#8211;<\/strong><strong>\u7ec6\u83cc\u5728\u5c0f\u578b\u751f\u7269\u53cd\u5e94\u5668\u57f9\u517b<\/strong><strong>&#8211;<\/strong><strong>\u7ec6\u83cc\u5728\u4e2d\u578b\u751f\u7269\u53cd\u5e94\u5668\u57f9\u517b<\/strong><\/span><\/p>\n<p style=\"font-size: 16px;white-space: normal;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><strong>\u4e2d\u6e38\u8fc7\u7a0b\uff1a\u53cd\u5e94\u5668\u4e2d\u7ec6\u83cc\u53ca<\/strong><strong>GMMA<\/strong><strong>\u4ea7\u7269\u3010<\/strong><strong>pH 6.7<\/strong><strong>\uff0c<\/strong><strong>30% DO<\/strong><strong>\uff0c<\/strong><strong>30\u2103<\/strong><strong>\uff0c<\/strong><strong>20<\/strong><strong>\u5c0f\u65f6\u540e<\/strong><strong>OD<\/strong><strong>\uff08<\/strong><strong>600<\/strong><strong>\uff09\u8fbe\u5230<\/strong><strong>35<\/strong><strong>\u3011<\/strong><\/span><\/p>\n<p style=\"font-size: 16px;white-space: normal;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><strong>\u4e0b\u6e38\u5206\u79bb\u53ca\u7eaf\u5316\uff1a<\/strong><strong>TFF<\/strong><strong>\u5fae\u6ee4\u53ca\u900f\u6790\uff08\u819c\u9762\u79ef<\/strong><strong>1.2m<sup>2<\/sup><\/strong><strong>\uff0c\u5b54\u5f84<\/strong><strong>0.2um<\/strong><strong>\uff09<\/strong><strong>-TFF<\/strong><strong>\u5fae\u6ee4\u53ca\u900f\u6790\uff08\u819c\u9762\u79ef<\/strong><strong>1.4m<sup>2<\/sup><\/strong><strong>\uff0c\u5b54\u5f84<\/strong><strong>300kDa<\/strong><strong>\uff09<\/strong><\/span><\/p>\n<p style=\"font-size: 16px;white-space: normal;\"><br  \/><\/p>\n<p style=\"font-size: 16px;white-space: normal;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><strong>B.&nbsp;<\/strong><strong>\u5e94\u7528\u4f53\u5916\u8f6c\u5f55\u7684<\/strong><strong>RNA<\/strong><strong>\u75ab\u82d7\u4ea7\u54c1<\/strong><\/span><\/p>\n<p style=\"font-size: 16px;white-space: normal;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><strong>\u4e0a\u6e38\u8fc7\u7a0b\uff1a\u6a21\u677f\u8d28\u7c92<\/strong><strong>DNA<\/strong><strong>\u7684\u5efa\u7acb<\/strong><strong>&#8211;<\/strong><strong>\u5728\u5927\u80a0\u6746\u83cc\u4e2d\u653e\u5927\u6a21\u677f\u8d28\u7c92<\/strong><strong>DNA-<\/strong><strong>\u8d28\u7c92<\/strong><strong>DNA<\/strong><strong>\u7684\u5185\u6bd2\u7d20\u7eaf\u5316<\/strong><strong>&#8211;<\/strong><strong>\u6a21\u677f\u8d28\u7c92<\/strong><strong>DNA<\/strong><strong>\u7684\u7ebf\u6027\u5316<\/strong><strong>&#8211;<\/strong><\/span><\/p>\n<p style=\"font-size: 16px;white-space: normal;line-height: 24px;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><strong>\u4e2d\u6e38\u8fc7\u7a0b\uff0c\u4e0b\u6e38\u5206\u79bb\u53ca\u7eaf\u5316\uff1a\u65e0\u7ec6\u80de\u8f6c\u5f55\uff08<\/strong><strong>5\u2019<\/strong><strong>\u7aef\u5e3d\u5b50\uff0c<\/strong><strong>37\u2103<\/strong><strong>\uff0c<\/strong><strong>4<\/strong><strong>\u5c0f\u65f6\uff09<\/strong><strong>-DNA<\/strong><strong>\u6d88\u5316\uff08<\/strong><strong>DNA<\/strong><strong>\u9176\uff0c<\/strong><strong>37\u2103<\/strong><strong>\uff0c\u534a\u5c0f\u65f6\uff09<\/strong><strong>&#8211;<\/strong><strong>\u5207\u5411\u6d41\u8fc7\u6ee4\u5206\u79bb<\/strong><strong>~2 PSI-<\/strong><strong>\u5207\u5411\u6d41\u8fc7\u6ee4\u6e17\u6790\uff0c<\/strong><strong>pH 8<\/strong><strong>\u5de6\u53f3<\/strong><strong>&nbsp;<\/strong><strong>\u8d28\u68c0\uff1a<\/strong><strong>HPL<\/strong><strong>\u53ca<\/strong><strong>MS<\/strong><\/span><\/p>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section class=\"_135editor\" style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"text-align:center;width: auto;\">\n<section style=\"display:inline-block;clear: both;\">\n<section style=\"padding: 6px 20px;background-image: url('http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/7-1558083948.jpeg');background-repeat: no-repeat;background-size: 100% 100%;letter-spacing: 1.5px;box-sizing: border-box;\">\n<section class=\"135brush\"  style=\"color: rgb(255, 255, 255);\">3. \u7528\u4e8e\u91cd\u7ec4\u75ab\u82d7\u751f\u4ea7\u7684\u4eba\u6e90\u5316\u3001\u9ad8\u6536\u7387\u9175\u6bcd\u5e73\u53f0<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p><br  \/><\/section>\n<p style=\"text-indent: 28px;line-height: 150%;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><span style=\"line-height: 150%;\">\u9175\u6bcd\u5e73\u53f0\u6240\u9762\u4e34\u7684\u4e3b\u8981\u6311\u6218\u5728\u4e8e\uff1a\u2460<\/span> <span style=\"line-height: 150%;\">\u4e0e\u4eba\u7c7b\u7ec6\u80de\u7cd6\u57fa\u5316\u6a21\u5f0f\u4e0d\u540c\uff1b\u2461<\/span> <span style=\"line-height: 150%;\">\u7279\u5f02\u6027\u86cb\u767d\u4ea7\u91cf\u8f83\u4f4e\uff1b\u2462 \u5bf9\u4e8e\u5927\u591a\u6570\u7528\u4e8e\u75ab\u82d7\u7684\u91cd\u7ec4\u86cb\u767d\u6297\u539f\u7ec4\u88c5\u6210VLP\u53ef\u4ee5\u6709\u6548\u63d0\u9ad8\u514d\u75ab\u539f\u6027\u3002\u4e3a\u4e86\u5e94\u5bf9\u8fd9\u4e9b\u6311\u6218\uff0c\u9ad8\u4ea7\u91cf\u7684\u4eba\u6e90\u5316\u9175\u6bcd\u8868\u8fbe\u5e73\u53f0\u6b63\u88ab\u8fc7\u5ea6\u8868\u8fbe\u7684\u65b9\u5f0f\u5f00\u53d1\u51fa\u6765\u3002\u901a\u8fc7\u5c06\u917f\u9152\u9175\u6bcd\uff08<\/span><span style=\"line-height: 150%;color: black;\">S. cerevisiae\uff09,\u6c49\u900a\u9175\u6bcd\uff08H. polymorpha\uff09\u4ee5\u53ca\u5df4\u65af\u5fb7\u6bd5\u6c0f\u9175\u6bcd\uff08P. pastoris\uff09<\/span> <span style=\"line-height: 150%;color: black;\">\u7b49\u8fdb\u884c\u8f6c\u57fa\u56e0\u6539\u9020\u5bfc\u81f4\u7cd6\u57fa\u5316\u901a\u8def\u6539\u53d8\uff0c<\/span><span style=\"line-height: 150%;\">\u6539\u53d8\u4e86\u9175\u6bcd\u4e2d\u9ad8\u7518\u9732\u7cd6\u7684\u8868\u8fbe\uff0c\u4ee5\u8fbe\u5230\u4eba\u7c7b\u590d\u5408\u7cd6\u7684\u6a21\u5f0f<span style=\"color: black;\">\u3002\u8fc4\u4eca\u4e3a\u6b62\uff0c\u7cd6\u5de5\u7a0b\u9175\u6bcd\u8868\u8fbe\u548c\u5206\u6ccc\u7684\u5927\u591a\u6570\u91cd\u7ec4\u86cb\u767d\u7684\u6ef4\u5ea6\u4f4e\u4e8e<\/span><\/span><span style=\"line-height: 150%;color: black;\">1gL<sup>-1<\/sup>\uff0c\u6709\u90e8\u5206\u57284-5gL<sup>-1<\/sup>\u3002\u8981\u8fbe\u5230\u975e\u7cd6\u5de5\u7a0bP. pastoris\u62a5\u9053\u768435 gL<sup>-1<\/sup>\u548c20gL<sup>-1<\/sup>\u7684\u5206\u6ccc\u6c34\u5e73\uff08\u5206\u522b\u91c7\u7528\u7532\u9187\u8bf1\u5bfc\u6cd5\u548c\u65e0\u7532\u9187\u6cd5\uff09\uff0c\u8fd8\u9700\u8981\u8fdb\u884c\u989d\u5916\u7684\u589e\u4ea7\u57fa\u56e0\u5de5\u7a0b\u3002\u5355\u4f53\u5916\u6e90\u6297\u539f\u7684\u514d\u75ab\u539f\u6027\u53ef\u901a\u8fc7\u4e0e\u9ad8\u514d\u75ab\u539f\u6027\u86cb\u767d\u7ed3\u6784\u57df\u8fde\u63a5\u6216\u4f7f\u7528\u9002\u5f53\u7684\u4f50\u5242\u800c\u589e\u5f3a\uff0c\u4ee5\u907f\u514d\u91cd\u7ec4\u6297\u539f\u86cb\u767d\u81ea\u7ec4\u88c5\u6210VLPs\u7684\u9700\u8981\u3002<\/span><\/span><\/p>\n<p style=\"line-height: 150%;\"><strong><span style=\"line-height: 150%;color: black;font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">3.1 \u4e0a\u6e38\u53ca\u4e2d\u6e38\u5904\u7406<\/span><\/strong><\/p>\n<p style=\"line-height: 150%;\"><span style=\"line-height: 150%;font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">\u4ee5\u9175\u6bcd\u4e3a\u57fa\u7840\u7684\u4eba\u5de5\u5316\u3001\u9ad8\u4ea7\u75ab\u82d7\u751f\u4ea7\uff08\u57fa\u4e8e\u917f\u9152\u9175\u6bcdHBsAg\u6297\u539f\u7684\u4ea7\u751f\uff09\u7684\u751f\u4ea7\u5de5\u827a\u5982\u56fe2\u6240\u793a\u3002\u5c06\u4eba\u6e90\u5316\u7684\u9175\u6bcd\u4e0e\u4f20\u7edf\u9175\u6bcd\u8fdb\u884c\u76f8\u4f3c\u7684\u7ec6\u80de\u5e93\u5efa\u7acb\u548c\u6269\u589e\u3002\u5bf9\u4e8e\u4eba\u6e90\u5316\u9175\u6bcd\u91cd\u7ec4\u6297\u539f\u7684\u751f\u4ea7\uff0c\u53ef\u4ee5\u91c7\u7528\u6807\u51c6\u7684\u9175\u6bcd\u751f\u7269\u5de5\u827a\u3002\u8d28\u7c92\u4e0a\u7684\u6297\u539f\u57fa\u56e0\u542f\u52a8\u5b50\u901a\u5e38\u6765\u6e90\u4e8e\u7cd6\u9175\u89e3\u672c\u6784\u57fa\u56e0\uff0c\u56e0\u6b64\u6297\u539f\u7684\u8868\u8fbe\u4e0e\u8461\u8404\u7cd6\u7684\u6d88\u8017\u548c\u751f\u7269\u91cf\u7684\u589e\u957f\u6210\u6b63\u6bd4\u3002\u4e3a\u4e86\u5c3d\u91cf\u51cf\u5c11\u80de\u5916\u8d28\u7c92\u4e22\u5931\u548c\u4ea7\u91cf\u4e0b\u964d\uff0c\u5728\u6b64\u8fc7\u7a0b\u4e2d\u76d1\u6d4b\u8d28\u7c92\u4fdd\u7559\u60c5\u51b5\uff0c\u5e76\u65bd\u52a0\u9009\u62e9\u538b\u529b\u3002\u6700\u540e\u7684\u53d1\u9175\u57f9\u517b\u6536\u83b7\u4e5f\u5e94\u6d4b\u5b9a\u5fae\u751f\u7269\u7684\u7eaf\u5ea6\u3002\u4ee5\u9175\u6bcd\u4e3a\u57fa\u7840\u7684\u751f\u4ea7\u7cfb\u7edf\u672c\u8eab\u4e0d\u4f1a\u6709\u4eba\u7c7b\u6216\u52a8\u7269\u75c5\u6bd2\u6c61\u67d3\u7684\u98ce\u9669\uff0c\u56e0\u6b64\uff0c\u5728\u8fd9\u65b9\u9762\u4e0d\u9700\u8981\u8fdb\u884c\u5177\u4f53\u7684\u68c0\u6d4b\u3002\u6574\u4e2a\u57f9\u517b\u5468\u671f\uff0c\u4ece\u89e3\u51bb\u5230\u5206\u6279\u53d1\u9175\u7ed3\u675f\u9700\u89811\u5468\u3002<\/span><\/p>\n<p style=\"line-height: 150%;\"><br  \/><\/p>\n<p style=\"line-height: 150%;\"><strong><span style=\"line-height: 150%;font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">3.2 \u5206\u79bb\u3001\u7ec6\u80de\u88c2\u89e3\u548c\u7eaf\u5316<\/span><\/strong><\/p>\n<p style=\"text-indent: 28px;line-height: 150%;\"><span style=\"line-height: 150%;font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">\u5229\u7528\u4eba\u6e90\u5316\u7684\u9175\u6bcd\u751f\u4ea7\u86cb\u767d\u8d28\u7684\u4e0b\u6e38\u8fc7\u7a0b\u7c7b\u4f3c\u4e8e\u4f20\u7edf\u9175\u6bcd\u53d1\u9175\u7684\u86cb\u767d\u8d28\u5206\u79bb\u7eaf\u5316\u8fc7\u7a0b\u3002\u4e0e\u52a8\u7269\u7ec6\u80de\u751f\u4ea7\u76f8\u6bd4\uff0c\u9175\u6bcd\u751f\u4ea7\u4e0b\u6e38\u7684\u5206\u79bb\u53ef\u4ee5\u5927\u5927\u7b80\u5316\uff0c\u4f7f\u5176\u66f4\u52a0\u7ecf\u6d4e\uff0c\u662f\u7531\u4e8e\uff1a\u2460\u9175\u6bcd\u6f5c\u5728\u7684\u9ad8\u91cd\u7ec4\u86cb\u767d\u8868\u8fbe\u548c\u5206\u6ccc\u91cf\uff1b\u2461\u5929\u7136\u5bbf\u4e3b\u7ec6\u80de\u9175\u6bcd\u86cb\u767d\u5206\u6ccc\u91cf\u8f83\u4f4e\u3002\u7136\u800c\uff0c\u7cd6\u5de5\u7a0b\u9175\u6bcd\u7684\u8868\u8fbe\u548c\u5206\u6ccc\u91cf\u4ecd\u9700\u63d0\u9ad8\uff0c\u624d\u80fd\u8fbe\u5230\u975e\u7cd6\u5de5\u7a0b\u9175\u6bcd\u7684\u6c34\u5e73\u3002\u56fe2\u662f\u4eba\u6e90\u9ad8\u4ea7\u9175\u6bcd\u548c\u6606\u866b\u7ec6\u80de\u5e73\u53f0\u86cb\u767d\u75ab\u82d7\u751f\u4ea7\u64cd\u4f5c\u65b9\u6848\u3002A\uff09\u5728\u4eba\u6e90\u5316\u9ad8\u4ea7\u9175\u6bcd\u4e2d\u751f\u4ea7\u91cd\u7ec4\u86cb\u767d\u75ab\u82d7\u3002\u4f46\u662f\uff0c\u8fd9\u79cd\u9ad8\u4ea7\u7684\u65b9\u6cd5\u8fd8\u6ca1\u6709\u5728\u751f\u4ea7\u89c4\u6a21\u4e0a\u5b9e\u65bd\u3002B\uff09\u5229\u7528MultiBac\u6606\u866b\u7ec6\u80de\u6746\u72b6\u75c5\u6bd2\u8868\u8fbe\u7cfb\u7edf\u5236\u5907\u91cd\u7ec4ADDomer\u591a\u6297\u539f\u75ab\u82d7\u3002\u57fa\u4e8e\u91cd\u7ec4HA\u86cb\u767d\u5728\u6d41\u611f\u75ab\u82d7\u751f\u4ea7\u4e2d\u7684\u8868\u8fbe\uff0c\u672c\u7814\u7a76\u5bf9\u8be5\u5de5\u827a\u8fdb\u884c\u4e86\u6269\u589e\u3002\u7070\u8272\u7bad\u5934\u8868\u793a\u75c5\u6bd2\u611f\u67d3\u524d\uff0c2500L\u751f\u4ea7\u7528\u751f\u7269\u53cd\u5e94\u5668\u4e2d\u591a\u4f59\u7684\u6606\u866b\u7ec6\u80de\u8fd4\u56de\u5230\u4e0a\u6e38\u8fc7\u7a0b\u3002<\/span><\/p>\n<p style=\"text-align: center;\"><img class=\"rich_pages\"          style=\"width: 100%;height: auto;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/10-1558083947.jpg\" alt=\"\u4f4e\u6210\u672c\u3001\u5feb\u901f\u751f\u4ea7\u75ab\u82d7\u7684\u65b0\u5174\u6280\u672f\" title=\"\u4f4e\u6210\u672c\u3001\u5feb\u901f\u751f\u4ea7\u75ab\u82d7\u7684\u65b0\u5174\u6280\u672f\"  \/><\/p>\n<p style=\"font-size: 16px;white-space: normal;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><strong>A.&nbsp;<\/strong><strong>\u4eba\u6e90\u5316\u9175\u6bcd\u7684\u91cd\u7ec4\u86cb\u767d\u75ab\u82d7\u4ea7\u54c1<\/strong><\/span><\/p>\n<p style=\"font-size: 16px;white-space: normal;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><strong>\u4e0a\u6e38\u8fc7\u7a0b\uff1a\u9175\u6bcd\u7ec6\u80de\u590d\u82cf<\/strong><strong>&#8211;<\/strong><strong>\u6447\u74f6\u57f9\u517b\u9175\u6bcd<\/strong><strong>-2~10L<\/strong><strong>\u9175\u6bcd\u7f50\u57f9\u517b\u9175\u6bcd<\/strong><strong>-20~300L<\/strong><strong>\u9175\u6bcd\u7f50\u57f9\u517b\u9175\u6bcd<\/strong><\/span><\/p>\n<p style=\"font-size: 16px;white-space: normal;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><strong>\u4e2d\u6e38\u8fc7\u7a0b\uff1a<\/strong><strong>1500L<\/strong><strong>\u9175\u6bcd\u7f50\uff0c<\/strong><strong>50~60g&nbsp;<\/strong><strong>\u7ec6\u80de<\/strong><strong>\/L<\/strong><\/span><\/p>\n<p style=\"font-size: 16px;white-space: normal;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><strong>\u4e0b\u6e38\u5206\u79bb\u53ca\u7eaf\u5316\uff1a\u5207\u5411\u6d41\u8fc7\u6ee4<\/strong><strong>&#8211;<\/strong><strong>\u9634\u79bb\u5b50\u4ea4\u6362\u5c42\u6790<\/strong><\/span><\/p>\n<p style=\"font-size: 16px;white-space: normal;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><strong>B.&nbsp;<\/strong><strong>\u6606\u866b\u7ec6\u80de<\/strong><strong>ADDmoer<\/strong><strong>\u75ab\u82d7\u4ea7\u54c1<\/strong><\/span><\/p>\n<p style=\"font-size: 16px;white-space: normal;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><strong>\u4e0a\u6e38\u8fc7\u7a0b\uff1a<\/strong><\/span><\/p>\n<p style=\"font-size: 16px;white-space: normal;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><strong>1<\/strong><strong>\uff09\u6606\u866b\u7ec6\u80de\u590d\u82cf<\/strong><strong>-0.5<\/strong><strong>\uff0c<\/strong><strong>1<\/strong><strong>\u6216<\/strong><strong>3L<\/strong><strong>\u6447\u74f6\u57f9\u517b\u7ec6\u80de<\/strong><strong>-30L<\/strong><strong>\u53cd\u5e94\u5668\u57f9\u517b\u7ec6\u80de<\/strong><strong>-300L<\/strong><strong>\u53cd\u5e94\u5668\u57f9\u517b\u7ec6\u80de<\/strong><\/span><\/p>\n<p style=\"font-size: 16px;white-space: normal;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><strong>2\uff09<\/strong><strong>\u75c5\u6bd2\u590d\u82cf<\/strong><strong>-3L<\/strong><strong>\u7ec6\u80de\u4e2d\u653e\u5927<\/strong><strong>-50~100L<\/strong><strong>\u751f\u7269\u53cd\u5e94\u5668\u4e2d\u653e\u5927<\/strong><\/span><\/p>\n<p style=\"font-size: 16px;white-space: normal;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><strong>\u4e2d\u6e38\u8fc7\u7a0b\uff1a<\/strong><strong>2500L<\/strong><strong>\u53cd\u5e94\u5668\uff0c\u57f9\u517b\u81f3\u7ec6\u80de\u6d53\u5ea6\u4e3a<\/strong><strong>0.5~0.9<\/strong><strong>\u00d7<\/strong><strong>10<sup>6<\/sup><\/strong><strong>\u7ec6\u80de<\/strong><strong>\/mL-<\/strong><strong>\u75c5\u6bd2<\/strong><strong>MOI0.1~1<\/strong><strong>\u611f\u67d3\u53cd\u5e94\u5668\u4e2d\u7684<\/strong><strong>2500L<\/strong><strong>\u7ec6\u80de<\/strong><\/span><\/p>\n<p style=\"font-size: 16px;white-space: normal;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><strong>\u4e0b\u6e38\u5206\u79bb\uff1a\u6df1\u5c42\u8fc7\u6ee4<\/strong><strong>-Triton X 100<\/strong><strong>\u88c2\u89e3\u7ec6\u80de<\/strong><\/span><\/p>\n<p style=\"font-size: 16px;white-space: normal;line-height: 24px;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><strong>\u4e0b\u6e38\u7eaf\u5316\uff1a\u9633\u79bb\u5b50\u4ea4\u6362\u5c42\u6790<\/strong><strong>&#8211;<\/strong><strong>\u4eb2\u548c\u8272\u8c31\u5c42\u6790<\/strong><strong>&#8211;<\/strong><strong>\u819c\u8fc7\u6ee4<\/strong><strong>&#8211;<\/strong><strong>\u8d85\u6ee4<\/strong><\/span><\/p>\n<p style=\"font-size: 16px;white-space: normal;line-height: 24px;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><strong><br  \/><\/strong><\/span><\/p>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section class=\"_135editor\" style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"text-align:center;width: auto;\">\n<section style=\"display:inline-block;clear: both;\">\n<section style=\"padding: 6px 20px;background-image: url('http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/7-1558083948.jpeg');background-repeat: no-repeat;background-size: 100% 100%;letter-spacing: 1.5px;box-sizing: border-box;\">\n<section class=\"135brush\"  style=\"color: rgb(255, 255, 255);\">4. \u7528\u4e8e\u91cd\u7ec4\u75ab\u82d7\u751f\u4ea7\u7684\u6606\u866b\u7ec6\u80de-\u6746\u72b6\u75c5\u6bd2\u5e73\u53f0<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p><br  \/><\/section>\n<h3 style=\"margin-bottom: 6px;text-indent: 28px;line-height: 150%;background: white;font-weight: bold;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><span style=\"line-height: 150%;font-weight: normal;\">\u6746\u72b6\u75c5\u6bd2\u662f\u4e00\u7c7b\u5927\u578b\u53cc\u94feDNA\u6606\u866b\u75c5\u6bd2\uff0c\u53ef\u5bb9\u7eb3\u591a\u79cd\u5916\u6765\u57fa\u56e0\uff0c\u5e38\u5728\u6606\u866b\u7ec6\u80de\u7cfb\u7684\u91cd\u7ec4\u86cb\u767d\u751f\u4ea7\u3002\u5206\u5b50\u514b\u9686\u65b9\u6cd5\u5728\u5546\u4e1a\u4e0a\u53ef\u7528\u6765\u5feb\u901f\u4ea7\u751f\u6746\u72b6\u75c5\u6bd2\u8f7d\u4f53\uff0c\u5305\u62ecCRISPR-Cas9\u6280\u672f\u3002\u7136\u800c\uff0c\u901a\u8fc7\u7a7a\u6591\u7eaf\u5316\u7b5b\u9009\u542b\u6709\u4eba\u4eec\u611f\u5174\u8da3\u57fa\u56e0\u7684\u91cd\u7ec4\u6746\u72b6\u75c5\u6bd2\u901a\u5e38\u9700\u89811\u5468\u5de6\u53f3\u7684\u65f6\u95f4\u3002\u4e3a\u4e86\u51cf\u8f7b\u83cc\u6591\u7684\u9009\u62e9\u548c\u7eaf\u5316\u7684\u7e41\u7410\u64cd\u4f5c\uff0c\u91cd\u7ec4\u6746\u72b6\u75c5\u6bd2\u901a\u5e38\u4f7f\u7528\u5927\u80a0\u6746\u83cc\u4e2d\u4eba\u5de5\u67d3\u8272\u4f53\u5f62\u5f0f\u7684\u6746\u72b6\u75c5\u6bd2\u7684\u524d\u4f53\u7ec6\u80de\u57fa\u56e0\u7ec4\u4ee5\u53caTn7\u8f6c\u5ea7\u5b50\u3002\u6606\u866b\u7ec6\u80de\u57f9\u517b\u5728\u91cd\u7ec4\u86cb\u767d\u751f\u4ea7\u65b9\u9762\u5177\u6709\u4ee5\u4e0b\u4f18\u52bf: \u2460 \u6bd4\u52a8\u7269\u7ec6\u80de\u7cfb\u5177\u6709\u66f4\u9ad8\u7684\u7a33\u5b9a\u6027\uff1b\u2461 \u6709\u6548\u7684\u6746\u72b6\u75c5\u6bd2\u8f7d\u4f53\u6784\u5efa\u6280\u672f\uff1b \u2462 S2\u7a33\u5b9a\u4fee\u9970\u7684\u6606\u866b\u7ec6\u80de\u53ef\u4ee5\u5728\u704c\u6ce8\u57f9\u517b\u7684\u8fde\u7eed\u6a21\u5f0f\u4e0b\u751f\u957f\uff1b\u2463 \u91cd\u7ec4\u86cb\u767d\u5728\u75ab\u82d7\u751f\u4ea7\u7684\u5de5\u4e1a\u89c4\u6a21\u9002\u7528\u6027\u662f\u7ecf\u8fc7\u9a8c\u8bc1\u4e86\u7684\uff1b<\/span><span style=\"line-height: 150%;color: rgb(30, 30, 30);background-image: initial;background-position: initial;background-size: initial;background-repeat: initial;background-attachment: initial;background-origin: initial;background-clip: initial;font-weight: normal;\">\u2464 \u6746\u72b6\u75c5\u6bd2\u4e0d\u7ed9\u4eba\u7c7b\u5065\u5eb7\u5e26\u6765\u98ce\u9669\u3002<\/span><\/span><\/h3>\n<h3 style=\"margin-bottom: 6px;line-height: 150%;background: white;text-indent: 2em;font-weight: bold;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><span style=\"line-height: 150%;color: rgb(30, 30, 30);background-image: initial;background-position: initial;background-size: initial;background-repeat: initial;background-attachment: initial;background-origin: initial;background-clip: initial;font-weight: normal;\">\u6606\u866b\u7ec6\u80de\u7684\u751f\u957f\u901f\u7387\u9ad8\u4e8e\u52a8\u7269\u7ec6\u80de\uff0c\u4f46\u4f4e\u4e8e\u9175\u6bcd\u6216\u7ec6\u83cc\u3002\u6606\u866b\u7ec6\u80de\u57f9\u517b\u5bf9\u91cd\u7ec4\u86cb\u767d\u7684\u4ea7\u751f\u6709\u4ee5\u4e0b\u9650\u5236: \u2460<\/span> <span style=\"line-height: 150%;color: rgb(30, 30, 30);background-image: initial;background-position: initial;background-size: initial;background-repeat: initial;background-attachment: initial;background-origin: initial;background-clip: initial;font-weight: normal;\">\u4e0d\u80fd\u5408\u6210\u54fa\u4e73\u52a8\u7269\u7279\u6709\u7684\u590d\u6742\u805a\u7cd6\u7ed3\u6784,\u7136\u800c\uff0c\u6700\u8fd1\u6746\u72b6\u75c5\u6bd2\u7cfb\u7edf\u901a\u8fc7\u6a21\u62df\u4eba\u7c7b\u7cd6\u57fa\u5316\u7684\u529f\u80fd\u6539\u8fdb\u4e86\u8fd9\u4e00\u70b9; \u2461<\/span> <span style=\"line-height: 150%;color: rgb(30, 30, 30);background-image: initial;background-position: initial;background-size: initial;background-repeat: initial;background-attachment: initial;background-origin: initial;background-clip: initial;font-weight: normal;\">\u75ab\u82d7\u751f\u4ea7\u6210\u672c\u8f83\u9ad8\uff0c\u4e0e\u52a8\u7269\u7ec6\u80de\u57f9\u517b\u75ab\u82d7\u751f\u4ea7\u6210\u672c\u76f8\u8fd1\u3002<\/span><\/span><\/h3>\n<p style=\"line-height: 150%;text-indent: 2em;\"><span style=\"line-height: 150%;color: #1E1E1E;background: white;font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">\u901a\u8fc7Multi-Bac\u4ee5\u53caADDomer \u591a\u86cb\u767d\u8868\u8fbe\u5e73\u53f0\uff0c\u53ef\u4ee5\u4f7f\u6606\u866b\u7ec6\u80de\u6746\u72b6\u75c5\u6bd2\u8868\u8fbe\u7cfb\u7edf\u5e94\u7528\u4e8e\u591a\u79cd\u75ab\u82d7\u7684\u751f\u4ea7\u3002Multi-Bac\u63d0\u4f9b\u4e86\u4e00\u79cd\u5728\u6606\u866b\u7ec6\u80de\u57f9\u517b\u4e2d\u4ea7\u751f\u91cd\u7ec4\u6746\u72b6\u75c5\u6bd2DNA\u8868\u8fbeVLP\u591a\u86cb\u767d\u590d\u5408\u7269\u7684\u7b80\u5355\u800c\u901a\u7528\u7684\u65b9\u6cd5\u3002ADDomer\u662f\u4ece\u4eba\u817a\u75c5\u6bd2\u8840\u6e053\u578bVLP\u4e2d\u63d0\u53d6\u7684\u5408\u6210\u591a\u86cb\u767d\u652f\u67b6\uff0c\u800c\u5229\u7528Multi-Bac\u53ef\u4ee5\u8fbe\u5230\u4e00\u4e2a\u8f83\u9ad8\u4ea7\u91cf\u3002ADDomer\u662f\u7531\u7ea660 kDa\u86cb\u767d\u4e9a\u57fa\uff08\u5355\u4f53\uff09\uff0c\u7ec4\u6210\u4e94\u805a\u4f53\uff0c\u7ea6300kDa\u300212\u4e2a\u8fd9\u6837\u7684\u5355\u4f53\u7ec4\u5408\u5f62\u6210ADDomer\uff0c\u5176\u603b\u5206\u5b50\u8d28\u91cf\u7ea6\u662f3.6 MDa\u3002ADDomer\u86cb\u767d\u652f\u67b6\u53ef\u5728\u5176\u8868\u9762\u663e\u793a\u591a\u8fbe360\u4e2a\u57fa\u56e0\u7f16\u7801\u7684\u6297\u539f\u51b3\u5b9a\u7c07\uff0c\u5305\u62ec\u7eaf\u5316\u6807\u7b7e\uff0c\u65b9\u4fbf\u4e0b\u6e38\u7eaf\u5316\u3002<\/span><\/p>\n<p style=\"text-indent: 28px;line-height: 150%;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><span style=\"line-height: 150%;\">ADDomer\u88ab\u9009\u4e3a\u4ee3\u8868\u6606\u866b\u7ec6\u80de\u6746\u72b6\u75c5\u6bd2\u8868\u8fbe\u7cfb\u7edf\u7684\u4e0b\u4e00\u4ee3\u75ab\u82d7\u5e73\u53f0\u662f\u56e0\u4e3a\uff1a\u2460<\/span> <span style=\"line-height: 150%;\">\u5b83\u662f\u9ad8\u5ea6\u53ef\u5b9a\u5236\u7684\u663e\u793a\u5404\u79cd\u6297\u539f\u591a\u80bd\uff0c\u86cb\u767d\u8d28\uff0c\u548c\u86cb\u767d\u8d28\u57df\u7684\u957f\u5ea6\u9ad8\u8fbe200\u4e2a\u6c28\u57fa\u9178\u5728\u5176\u8868\u9762; \u2461<\/span> <span style=\"line-height: 150%;\">\u53ef\u4f7f\u7528MultiBac\u7cfb\u7edf\u5feb\u901f\u91cd\u65b0\u914d\u7f6e; \u2462 \u5b83\u7531\u817a\u75c5\u6bd2\u620a\u57fa\u86cb\u767d\u7684\u62f7\u8d1d\u7ec4\u6210\uff0c\u8fd9\u4e9b\u62f7\u8d1d\u53ef\u4ee5\u81ea\u53d1\u7ec4\u88c5\u6210\u9ad8\u7a33\u5b9a\u6027\u7684VLPs; \u2463<\/span> <span style=\"line-height: 150%;\">\u5b83\u7684\u5927\u5c0f\u4e0e\u75c5\u6bd2\u76f8\u4f3c\uff0c\u589e\u5f3a\u4e86\u5176\u514d\u75ab\u539f\u6027;<\/span><span style=\"line-height: 150%;background: white;\">\u2464<\/span> <span style=\"line-height: 150%;\">\u5b83\u53ef\u4ee5\u5305\u542b\u7c7b\u4f3c\u4f50\u5242\u7684\u8868\u4f4d; \u2465<\/span> <span style=\"line-height: 150%;\">\u5b83\u662f\u975e\u590d\u5236\u6027\u7684\uff0c\u4e0d\u643a\u5e26\u9057\u4f20\u7269\u8d28\uff1b<\/span> <span style=\"line-height: 150%;\">\u2466<\/span> <span style=\"line-height: 150%;\">\u5b83\u53ef\u4ee5\u5f88\u5bb9\u6613\u5730\u4ee5\u5de5\u4e1a\u89c4\u6a21\u751f\u4ea7; \u2467<\/span> <span style=\"line-height: 150%;\">\u5b83\u662f\u70ed\u7a33\u5b9a\u7684\uff0c\u4e0d\u53d7\u51b7\u94fe\u7684\u5f71\u54cd\uff0c\u8fd9\u5728\u4e2d\u4f4e\u6536\u5165\u56fd\u5bb6\u662f\u4e00\u4e2a\u4f18\u52bf\u3002<\/span><\/span><\/p>\n<p style=\"line-height: 150%;\"><span style=\"line-height: 150%;font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">\u672c\u6587\u63cf\u8ff0\u4e86\u5728\u6606\u866b\u7ec6\u80de\u4e2d\u5236\u9020\u591a\u86cb\u767d\u590d\u5408\u7269(\u5982ADDomer)\u7684\u8fc7\u7a0b\u3002\u8be5ADDomer\u751f\u4ea7\u5de5\u827a\u7684\u6269\u5927\u662f\u57fa\u4e8e\u6d41\u611f\u75c5\u6bd2\u8840\u51dd\u7d20(HA)\u751f\u4ea7Flublok\u75ab\u82d7\uff0c\u56e0\u4e3aHA\u591a\u805a\u4f53\u7684\u5927\u5c0f\u4e0eADDomer\u7c7b\u4f3c\u3002<\/span><\/p>\n<p style=\"line-height: 150%;\"><strong><span style=\"line-height: 150%;font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">4.1 \u4e0a\u6e38\u53ca\u4e2d\u6e38\u5904\u7406<\/span><\/strong><\/p>\n<p style=\"text-indent: 28px;line-height: 150%;\"><span style=\"line-height: 150%;font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">ADDomer\u7531MultiBac\u8f7d\u4f53\u5728Hi5\u6216Sf21\u4e2d\u8868\u8fbe\uff0c\u4e3a\u6b64\uff0c\u7ec6\u80de\u548c\u6746\u72b6\u75c5\u6bd2\u88ab\u6269\u5927\u3002<\/span><\/p>\n<p style=\"text-indent: 28px;line-height: 150%;\"><span style=\"line-height: 150%;font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">\u968f\u7740\u6606\u866b\u7ec6\u80de\u5728\u4e2d\u5c0f\u578b\u751f\u7269\u53cd\u5e94\u5668\u4e2d\u88ab\u653e\u5927\uff0c\u7ec6\u80de\u4f1a\u88ab\u8f6c\u79fb\u5230\u751f\u4ea7\u751f\u7269\u53cd\u5e94\u5668\u4e2d\u3002\u5728\u611f\u67d3\u4e4b\u524d\uff0c\u751f\u4ea7\u751f\u7269\u53cd\u5e94\u5668\u4e2d\u7684\u591a\u4f59\u7ec6\u80de\u4f1a\u88ab\u9001\u5230\u4e0a\u6e38\u8fc7\u7a0b\uff0c\u5982\u56fe2B\u4e2d\u7070\u8272\u7bad\u5934\u6240\u793a\u3002\u5f53\u7ec6\u80de\u5bc6\u5ea6\u5230\u8fbe0.5-0.910<sup>6<\/sup> \u4e2a\/mL \u65f6\uff0c\u52a0\u5165MOI\u4e3a 0.1-1\u7684\u6746\u72b6\u75c5\u6bd2\uff0c\u8fd9\u6837\u7ec6\u80de\u7fa4\u5728\u611f\u67d3\u540e\u81f3\u5c11\u53ef\u4ee5\u8fbe\u5230\u53cc\u500d\u3002\u52a0\u5165\u7684\u75c5\u6bd2\u611f\u67d3\u7ec6\u80de\uff0c\u800c\u7ec6\u80deADDomer\u5c31\u4f1a\u5728\u7ec6\u80de\u5185\u88ab\u8868\u8fbe\u5e76\u4fdd\u7559\u4e0b\u6765\u3002\u5229\u7528\u8868\u8fbe\u9ec4\u8272\u8367\u5149\u6807\u8bb0\u7269\u6216\u7ec6\u80de\u5bc6\u5ea6\u6bcf12\u621624\u5c0f\u65f6\u76d1\u6d4b\u4e00\u6b21\u75c5\u6bd2\u7684\u8868\u8fbe\u3002\u4e00\u65e6\u8868\u8fbe\u7a33\u5b9a\u6216\u7ec6\u80de\u5bc6\u5ea6\u505c\u6b62\u589e\u52a024\u5c0f\u65f6\u540e\uff0c\u4fbf\u4f1a\u6536\u83b7\u7ec6\u80de\u3002<\/span><\/p>\n<p style=\"line-height: 150%;\"><strong><span style=\"line-height: 150%;font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">4.2 \u5206\u79bb\u53ca\u7eaf\u5316<\/span><\/strong><\/p>\n<p style=\"text-indent: 28px;line-height: 150%;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><span style=\"line-height: 150%;\">\u611f\u67d3\u540e\u7684\u7ec6\u80de\u901a\u8fc7\u79bb\u5fc3\u6536\u83b7\u3002\u901a\u8fc7\u8868\u9762\u6d3b\u6027\u5242\uff08Triton X-100\uff0c\u8131\u6c27\u80c6\u9178\u94a0\u7b49<\/span> <span style=\"line-height: 150%;\">\uff09\u5c06ADDomer\u4ece\u7ec6\u80de\u4e2d\u63d0\u53d6\u51fa\u6765\uff0c\u7136\u540e\u7528\u6df1\u5ea6\u8fc7\u6ee4\u6f84\u6e05\u3002\u5728\u6df1\u5ea6\u8fc7\u6ee4\u8fc7\u7a0b\u4e2d\uff0c\u7ec6\u80de\u788e\u7247\u548c\u5176\u4ed6\u6c61\u67d3\u7269\u88ab\u622a\u7559\uff0c\u542b\u6709ADDomer\u7684\u6eb6\u6db2\u5219\u901a\u8fc7\u8fc7\u6ee4\u5668\u6f84\u6e05\u3002<\/span><\/span><\/p>\n<p style=\"text-indent: 28px;line-height: 150%;\"><span style=\"line-height: 150%;font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">\u6765\u6e90\u4e8e\u6df1\u5ea6\u8fc7\u6ee4\u7684\u6f84\u6e05\u6db2\u901a\u8fc7\u79bb\u5b50\u4ea4\u6362\u8272\u8c31\u67f1\u7eaf\u5316\u3002ADDomer\u86cb\u767d\u4f1a\u7ed3\u5408\u5728\u67f1\u4e0a\uff0cpH\u4f4e\u4e8eADDomer\u7b49\u7535\u70b9\u7684\u7f13\u51b2\u6db2\u4f1a\u4f7f\u5176\u96c6\u5408\u5728\u67f1\u4e0a\uff0c\u7136\u540e\u901a\u8fc7pH\u9ad8\u4e8eADDomer\u7b49\u7535\u70b9\u7684\u7f13\u51b2\u6db2\u5c06\u5176\u6d17\u8131\u3002\u7136\u540e\u53ef\u4ee5\u6dfb\u52a0\u4e00\u6b65\u9488\u5bf9ADDomer\u6807\u7b7e\u7684\u8272\u8c31\u7eaf\u5316\u8fdb\u884c\u8fdb\u4e00\u6b65\u7eaf\u5316\uff0c\u6807\u7b7e\u662f\u5728\u5206\u5b50\u514b\u9686\u9636\u6bb5\u6dfb\u52a0\u7684\u3002\u5728\u7ecf\u8fc7\u4e24\u6b65\u67f1\u5c42\u6790\u4e4b\u540e\uff0c\u63a5\u7740\u4f7f\u7528Q\u819c\u53bb\u9664\u6b8b\u7559\u7684DNA\u3002\u6700\u540e\u7684\u7f13\u51b2\u6db2\u6210\u5206\u5c31\u662f\u5176\u6700\u7ec8\u4f7f\u7528\u7684\u6210\u5206\u3002<\/span><\/p>\n<p style=\"line-height: 150%;\"><br  \/><\/p>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section class=\"_135editor\" style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"text-align:center;width: auto;\">\n<section style=\"display:inline-block;clear: both;\">\n<section style=\"padding: 6px 20px;background-image: url('http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/7-1558083948.jpeg');background-repeat: no-repeat;background-size: 100% 100%;letter-spacing: 1.5px;box-sizing: border-box;\">\n<section class=\"135brush\"  style=\"color: rgb(255, 255, 255);\">5. \u5916\u819c\u56ca\u6ce1\u75ab\u82d7\uff0c\u819c\u6297\u539f\u75ab\u82d7\u751f\u4ea7\u7684\u901a\u7528\u6a21\u5757<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 32px;line-height: 150%;\"><span style=\"line-height: 150%;\">\u5916\u819c\u56ca\u6ce1<\/span><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><span style=\"line-height: 150%;\">\uff08OMVs\uff09\u662f\u6240\u6709\u9769\u5170\u6c0f\u9634\u6027\u83cc\u5728\u751f\u957f\u8fc7\u7a0b\u4e2d\u81ea\u7136\u4ea7\u751f\u7684\u9769\u5170\u6c0f\u9633\u6027\u83cc\u3001\u5206\u679d\u6746\u83cc\u3001\u548c\u53e4\u83cc\u4e5f\u80fd\u81ea\u7136\u91ca\u653e\u51fa\u5916\u819c\u56ca\u6ce1(OMV)\u3002<\/span> <span style=\"line-height: 150%;\">\u5916\u819c\u56ca\u6ce1(OMV)\u662f\u5185\u541e\u8d77\u6e90\u7684\u7403\u5f62\u5b9e\u4f53\uff0c\u76f4\u5f84\u901a\u5e38\u572820-250nm\u4e4b\u95f4\u3002\u8fd9\u4e9b\u5177\u6709\u5d4c\u5165\u86cb\u767d\u7684\u75c5\u6bd2\u5927\u5c0f\u7684\u8102\u8d28\u53cc\u5c42\u56ca\u6ce1\u5177\u6709\u5f88\u5f3a\u7684\u514d\u75ab\u539f\u6027\u3002\u57fa\u4e8eOMV\u7684\u75ab\u82d7\u53ef\u4ee5\u901a\u8fc7\u4ee5\u4e0b\u51e0\u79cd\u65b9\u5f0f\u751f\u4ea7\uff1aa\uff09\u57f9\u517b\u5929\u7136\u5206\u6cccOMV\u7684\u91ce\u751f\u578b\u7ec6\u83cc;<sup>&nbsp; <\/sup>b\uff09\u5728EDTA\u5b58\u5728\u4e0b\u4f7f\u7528\u8131\u6c27\u80c6\u9178\u94a0\u8fdb\u884c\u6d17\u6da4\u5242\u63d0\u53d6; c\uff09\u57f9\u517b\u9057\u4f20\u6539\u53d8\u7684\u7ec6\u83cc\u4ee5\u589e\u5f3aOMV\u7684\u4ea7\u751f\uff0c\u6d17\u6da4\u5242\u63d0\u53d6\u53bb\u9664\u4e86\u53cd\u5e94\u539f\u6027\u6216\u6bd2\u6027\u8102\u591a\u7cd6\uff0c\u4f46\u6709\u4ee5\u4e0b\u7f3a\u70b9\uff1a1\uff09\u5e26\u8d1f\u7535\u7684\u8868\u9762\u6297\u539f\u7684\u4e22\u5931<\/span> <span style=\"line-height: 150%;\">\u30022\uff09OMV\u4f1a\u805a\u96c6\u5e76\u4e14\u5927\u5c0f\u4e0d\u5747\u5300\uff0c\u5728\u65e0\u83cc\u8fc7\u6ee4\u671f\u95f4\u635f\u5931\u4e00\u5c0f\u90e8\u5206OMV; 3\uff09\u7531\u4e8e\u7ec6\u83cc\u7ec6\u80de\u88c2\u89e3\u800c\u4f7fOMV\u75ab\u82d7\u88ab\u7ec6\u80de\u8d28\u86cb\u767d\u6c61\u67d3\uff0c\u4e3a\u4e86\u514b\u670d\u8fd9\u4e9b\u9650\u5236\uff0c\u4ea7\u751fOMV\u7684\u7ec6\u83cc\u7ecf\u57fa\u56e0\u5de5\u7a0b\u6539\u9020\u4ee5\u6539\u5584OMV\u75ab\u82d7\u7684\u4ea7\u751f\u3002\u8fd9\u4e9b\u57fa\u56e0\u5de5\u7a0b\u6539\u9020\u53ef\u5305\u62ec\uff1a1\uff09\u6539\u53d8\u7ec6\u83cc\u8102\u591a\u7cd6\u751f\u7269\u5408\u6210\u9014\u5f84\u4ee5\u964d\u4f4e\u5185\u6bd2\u6027\u548c\u53cd\u5e94\u539f\u6027; 2\uff09\u6297\u539f\u8fc7\u5ea6\u8868\u8fbe; 3\uff09\u540c\u65f6\u8868\u8fbe\u591a\u79cd\u6297\u539f\u548c\u6297\u539f\u53d8\u5f02\u4f53; 4\uff09\u5728\u5916\u819c\u4e2d\u4fdd\u7559\u5206\u6ccc\u7684\u6297\u539f; 5\uff09\u901a\u8fc7\u53bb\u9664\u5916\u819c\u951a\u86cb\u767d\u6765\u589e\u5f3aOMV\u7684\u4ea7\u751f; 6\uff09\u53bb\u9664\u53ef\u80fd\u5f15\u53d1\u4e0d\u5e0c\u671b\u7684\u514d\u75ab\u53cd\u5e94\u7684\u514d\u75ab\u8c03\u8282\u6210\u5206; 7\uff09\u5305\u88f9\u6765\u81ea\u5bbf\u4e3bOMV\u4ea7\u751f\u83cc\u682a\u4ee5\u5916\u7684\u75c5\u539f\u4f53\u7684\u6297\u539f\uff0c\u5185\u5916\u7ec6\u80de\u58c1\u819c\u7684\u5b8c\u6574\u6027\u548c\u9644\u7740\u901a\u5e38\u7531Tol-Pal\u7cfb\u7edf\u8c03\u8282\uff0c\u5e76\u4e14\u53ef\u4ee5\u5229\u7528\u8be5\u9014\u5f84\u7684\u4fee\u9970\u52a0\u5f3aOMV\u7684\u4ea7\u751f\u3002<\/span><\/span><\/p>\n<p style=\"text-indent: 32px;line-height: 150%;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><span style=\"line-height: 150%;\">\u819c\u6297\u539f\u7684\u5e7f\u8c31\u6a21\u5757\uff08GMMA\uff09\u662fOMV\uff0c\u7ecf\u8fc7\u6539\u9020\u53ef\u589e\u5f3a\u5929\u7136OMV\u7684\u5f62\u6210\uff08\u901a\u8fc7\u5220\u9664\u8111\u819c\u708e\u7403\u83cc\u4e2d\u7684gna33\u548c\u5fd7\u8d3a\u6c0f\u83cc\u548c\u6c99\u95e8\u6c0f\u83cc\u4e2d\u7684tolR\uff09\uff0c\u964d\u4f4e\u53cd\u5e94\u539f\u6027\u548c\u6bd2\u6027\uff08\u901a\u8fc7\u6539\u53d8\u8102\u8d28A\u7684\u9170\u5316\u6a21\u5f0f\uff09\u548c\u8868\u8fbe\u514d\u75ab\u539f\u6027\u6297\u539f\u3002GMMA\u542b\u6709\u4e0e\u75c5\u539f\u4f53\u76f8\u5173\u7684\u5206\u5b50\u6a21\u5f0f\uff0c\u5305\u62ecToll\u6837\u53d7\u4f53\u914d\u4f53\uff0c\u5b83\u4eec\u81ea\u8eab\u53ef\u4ee5\u4f5c\u4e3a\u5f15\u53d1\u514d\u75ab\u5e94\u7b54\u4e2d\u7684\u4f50\u5242\u3002GMMA\u5177\u6709\u9ad8\u5ea6\u514d\u75ab\u539f\u6027\uff0c\u6709\u6548\uff0c\u53cd\u5e94\u539f\u6027\u4f4e\uff0c\u5b89\u5168\uff0c\u5e76\u4e14\u53ef\u4ee5\u5728\u53ef\u6269\u5c55\u7684\u8fc7\u7a0b\u4e2d\u4ee5\u4f4e\u6210\u672c\u5236\u9020\u3002 GMMA\u7684\u4e3b\u8981\u9650\u5236\u662f\u5b83\u4e0d\u80fd\u6267\u884c\u7c7b\u4f3c\u4eba\u7684\u7ffb\u8bd1\u540e\u4fee\u9970\uff08\u4f8b\u5982\uff0c\u7cd6\u57fa\u5316\uff09\u3002\u4f7f\u7528\u5fd7\u8d3a\u6c0f\u5fd7\u8d3a\u6c0f\u83cc\u548c\u975e\u4f24\u5bd2\u6c99\u95e8\u6c0f\u83cc\u75ab\u82d7\u5df2\u7ecf\u7b26\u5408\u4e86GMP\u8d28\u91cf\u3002\u8fd9\u91cc\u63cf\u8ff0\u4e86\u5728Songeli\u5fd7\u8d3a\u83cc\u4e2d\u751f\u4ea7\u7b26\u5408GMP\u8d28\u91cf\u7684GMMA\uff0c\u56e0\u4e3a\u8fd9\u79cd\u57fa\u4e8eGMMA\u7684\u75ab\u82d7\u662f\u9488\u5bf9\u6700\u5148\u8fdb\u7684\u75ab\u82d7\u5f00\u53d1\u7684\u9636\u6bb5\uff0c\u5728\u4e34\u5e8a\u8bd5\u9a8c\u4e2d\u8bc1\u5b9e\u4e86\u5176\u5bf9\u4e8e\u6210\u5e74\u4eba\u7684\u5b89\u5168\u6027\u548c\u514d\u75ab\u539f\u6027\u3002\u4ece\u7528\u4e8e\u53d1\u9175\u7684\u63a5\u79cd\u7269\u89e3\u51bb\u5230\u6700\u7ec8\u7eaf\u5316\u7684GMMA\u7684\u751f\u4ea7\u65f6\u95f4\u4e3a3\u5929\uff0c\u56e0\u6b64\uff0c\u6839\u636e\u75ab\u82d7\u5242\u91cf\u7684\u5927\u5c0f\uff0c\u5177\u6709500L\u53d1\u9175\u7f50\u7684\u76f8\u5bf9\u5c0f\u7684\u751f\u4ea7\u8bbe\u65bd\u6bcf\u5e74\u53ef\u4ea7\u751f\u8d85\u8fc71\u4ebf\u5242\u75ab\u82d7\u3002<\/span><sup> <\/sup><span style=\"line-height: 150%;\">\u4ee5GMMA\u4e3a\u4f8b\u5236\u5907OMV\u75ab\u82d7\u7684\u64cd\u4f5c\u65b9\u6848\u5982\u4e0b\u56fe1\u6240\u793a\u3002<\/span><\/span><\/p>\n<p style=\"text-align: center;\"><img class=\"rich_pages\"        style=\"width: 556px;height: auto;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/5-1558083947.jpg\" alt=\"\u4f4e\u6210\u672c\u3001\u5feb\u901f\u751f\u4ea7\u75ab\u82d7\u7684\u65b0\u5174\u6280\u672f\" title=\"\u4f4e\u6210\u672c\u3001\u5feb\u901f\u751f\u4ea7\u75ab\u82d7\u7684\u65b0\u5174\u6280\u672f\"  \/><\/p>\n<p><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><strong>\u7cfb\u7edf\u5212\u5206<\/strong><\/span><\/p>\n<p><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><strong>\u751f\u7269\u5904\u7406\uff1a\u4e0a\u6e38\u8fc7\u7a0b<\/strong><strong>&#8211;<\/strong><strong>\u4e2d\u6e38\u8fc7\u7a0b<\/strong><strong>&#8211;<\/strong><strong>\u4e0b\u6e38\u5206\u79bb\u53ca\u7eaf\u5316<\/strong><\/span><\/p>\n<p><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><strong>\u914d\u4f0d\uff0c\u8d28\u91cf\u63a7\u5236\u53ca\u7f50\u88c5\uff1a\u914d\u4f0d<\/strong><strong>&#8211;<\/strong><strong>\u7f50\u88c5<\/strong><strong>&#8211;<\/strong><strong>\u52a0\u76d6\u53ca\u5bc6\u5c01<\/strong><\/span><\/p>\n<p><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><strong>\u7ed3\u675f\u53ca\u5305\u88c5\uff1a\u8d34\u6807<\/strong><strong>&#8211;<\/strong><strong>\u8d28\u91cf\u63a7\u5236<\/strong><strong>&#8211;<\/strong><strong>\u5305\u88c5<\/strong><\/span><\/p>\n<p style=\"text-indent: 32px;line-height: 150%;\"><br  \/><\/p>\n<p style=\"line-height: 150%;\"><strong><span style=\"line-height: 150%;font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">5.1.\u4e0a\u6e38\u548c\u4e2d\u6e38\u5904\u7406<\/span><\/strong><\/p>\n<p style=\"text-indent: 24px;line-height: 150%;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><span style=\"line-height: 150%;\">\u5bf9\u611f\u5174\u8da3\u7684\u7ec6\u83cc\u83cc\u682a\u8fdb\u884c\u57fa\u56e0\u5de5\u7a0b\u6539\u9020\u4ee5\u83b7\u5f97GMMA\u75ab\u82d7\u751f\u4ea7\u6240\u9700\u7684\u6027\u8d28\u3002\u4e3a\u6b64\uff0c\u5728Songeli\u5fd7\u8d3a\u6c0f\u83cc\u4e2d\uff0ctolR\uff0cgalU\u548cmsbB1\u57fa\u56e0\u88ab\u6297\u751f\u7d20\u9009\u62e9\u6807\u8bb0\u53d6\u4ee3\u3002<\/span><sup><span style=\"line-height: 150%;font-size: 14px;\">[91]<\/span><\/sup><span style=\"line-height: 150%;\">\u5bf9\u4e8e\u6c99\u95e8\u6c0f\u83cc\u4e2d\u7684GMMA\u75ab\u82d7\u751f\u4ea7\uff0c\u53ef\u4ee5\u5220\u9664tolR\uff0cmsbB\uff0chtrB\u548cpagP\u57fa\u56e0\uff0c\u5e76\u7528\u6297\u751f\u7d20\u9009\u62e9\u6807\u8bb0\u53d6\u4ee3\u3002<\/span><sup><span style=\"line-height: 150%;font-size: 14px;\">[90]<\/span><\/sup><span style=\"line-height: 150%;\">\u5c06\u83b7\u5f97\u7684\u7ec6\u83cc\u83cc\u682a\u50a8\u5b58\u5728\u53cc\u5c42\u7ec6\u80de\u5e93\u4e2d\uff0c\u7136\u540e\u6269\u589e\u7528\u4e8e\u751f\u4ea7\u3002\u4e0e\u52a8\u7269\u7ec6\u80de\u76f8\u6bd4\uff0c\u7ec6\u83cc\u751f\u957f\u66f4\u5feb\uff0c\u53ef\u4ee5\u5728\u66f4\u5927\u5bb9\u91cf\u7684\u751f\u7269\u53cd\u5e94\u5668\u4e2d\u57f9\u517b\uff0c\u56e0\u4e3a\u4e0e\u52a8\u7269\u7ec6\u80de\u76f8\u6bd4\uff0c\u7ec6\u83cc\u7ec6\u80de\u66f4\u575a\u56fa\uff0c\u53ef\u627f\u53d7\u66f4\u9ad8\u7684\u538b\u529b\u548c\u6405\u62cc\u901f\u7387\u3002<\/span><sup><span style=\"line-height: 150%;font-size: 14px;\">[13]<\/span><\/sup><\/span><\/p>\n<p style=\"text-indent: 24px;line-height: 150%;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><span style=\"line-height: 150%;\">\u4e00\u65e6\u83b7\u5f97\u6240\u9700\u7684\u63a5\u79cd\u4f53\u79ef\uff0c\u5c06\u7ec6\u83cc\u63a5\u79cd\u5230\u8865\u6599\u5206\u6279\u751f\u4ea7\u751f\u7269\u53cd\u5e94\u5668\u4e2d\u3002\u7ec6\u83cc\u53d1\u9175\u7f50\u53ef\u4ee5\u6709\u51e0\u767e\u7acb\u65b9\u7c73\u7684\u4f53\u79ef\uff0c\u8fd8\u63cf\u8ff0\u4e86\u4f53\u79ef\u8fbe2000\u7acb\u65b9\u7c73\u7684\u53d1\u9175\u7f50\u3002<\/span><sup><span style=\"line-height: 150%;font-size: 14px;\">[13]<\/span><\/sup><span style=\"line-height: 150%;\">\u4e00\u65e6\u7ec6\u83cc\u5230\u8fbe\u9759\u6b62\u751f\u957f\u671f\uff0c\u8089\u6c64\u5c31\u8f6c\u79fb\u5230\u4e0b\u6e38\u7eaf\u5316\u9636\u6bb5\u3002<\/span><\/span><\/p>\n<p style=\"line-height: 150%;\"><strong><span style=\"line-height: 150%;font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">5.2.\u5206\u79bb\u548c\u7eaf\u5316<\/span><\/strong><\/p>\n<p style=\"text-indent: 32px;line-height: 150%;\"><span style=\"line-height: 150%;font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">\u53d1\u9175\u8fc7\u7a0b\u4e2d\u4ea7\u751f\u7684GMMA\u88ab\u91ca\u653e\u5230\u53d1\u9175\u8089\u6c64\u4e2d\uff0c\u5e76\u4f7f\u7528\u4e24\u4e2a\u8fde\u7eed\u7684TFF\u6b65\u9aa4\u4e0e\u5176\u4ed6\u8089\u6c64\u7ec4\u5206\u5206\u79bb\uff1a\u5fae\u6ee4\u548c\u8d85\u6ee4\u3002\u5728\u7b2c\u4e00\u4e2a\u5fae\u6ee4\u6b65\u9aa4\u4e2d\uff0c\u751f\u7269\u53cd\u5e94\u5668\u8fde\u63a5\u5230TFF\u7cfb\u7edf\u5e76\u7528\u4f5c\u518d\u5faa\u73af\u7f50\uff0c\u4ece\u800c\u4ece\u8089\u6c64\u4e2d\u9664\u53bb\u7ec6\u83cc\u5e76\u4e14GMMA\u4fdd\u7559\u5728\u8089\u6c64\u4e2d\u3002\u5c06\u57f9\u517b\u4e0a\u6e05\u6db2\u6d53\u7f29\u4e09\u6b21\uff0c\u7136\u540e\u76f8\u5bf9\u4e8e\u4e09\u500d\u6d53\u7f29\u7684\u4e0a\u6e05\u6db2\u4f53\u79ef\uff0c\u5bf9\u4e94\u500d\u4f53\u79ef\u7684\u7f13\u51b2\u6db2\u8fdb\u884c\u4e0d\u8fde\u7eed\u7684\u6e17\u6ee4\u3002\u5728\u7b2c\u4e8c\u6b21\u8d85\u6ee4\u6b65\u9aa4\u4e2d\uff0c\u9664\u53bb\u5927\u90e8\u5206\u53ef\u6eb6\u6027\u86cb\u767d\u8d28\u548c\u6838\u9178\u3002\u63a5\u4e0b\u6765\uff0c\u4f7f\u7528\u918b\u9178\u7ea4\u7ef4\u7d20\u706d\u83cc\u8fc7\u6ee4\u5668\u5c06GMMA\u6eb6\u6db2\u6d53\u7f29\u81f3\u914d\u5236\u8fc7\u7a0b\u6240\u9700\u7684\u6c34\u5e73\u3002<\/span><\/p>\n<p style=\"text-indent: 32px;line-height: 150%;\"><span style=\"line-height: 150%;font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">\u5236\u5907\u819c\u6297\u539f\uff08GMMA\uff09\u548cRNA\u75ab\u82d7\u901a\u7528\u6a21\u5757\u7684\u64cd\u4f5c\u65b9\u6848\u89c1\u56fe1\u3002 A\uff09\u4f7f\u7528\u9769\u5170\u6c0f\u9634\u6027\u7ec6\u83cc\u751f\u4ea7GMMA\u75ab\u82d7\u3002 GMMA\u5728\u7ec6\u83cc\u57f9\u517b\u8fc7\u7a0b\u4e2d\u4e0d\u65ad\u91ca\u653e\uff0c\u5e76\u4f7f\u7528\u4e24\u4e2a\u8fde\u7eed\u7684TFF\u6b65\u9aa4\u8fdb\u884c\u5206\u79bb\u3002B\uff09\u4f7f\u7528\u4f53\u5916\u8f6c\u5f55\u751f\u4ea7RNA\u75ab\u82d7\u3002<\/span><\/p>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section class=\"_135editor\" style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"text-align:center;width: auto;\">\n<section style=\"display:inline-block;clear: both;\">\n<section style=\"padding: 6px 20px;background-image: url('http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/7-1558083948.jpeg');background-repeat: no-repeat;background-size: 100% 100%;letter-spacing: 1.5px;box-sizing: border-box;\">\n<section class=\"135brush\"  style=\"color: rgb(255, 255, 255);\">6. RNA\u75ab\u82d7\u751f\u4ea7<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 32px;line-height: 150%;\"><span style=\"line-height: 150%;font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">\u6838\u9178\uff08DNA\u6216RNA\uff09\u75ab\u82d7\u7f16\u7801\u6297\u539f\uff0c\u5e76\u4e14\u8fd9\u79cd\u75ab\u82d7\u7f16\u7801\u7684\u6297\u539f\u53ef\u4ee5\u5728\u60a3\u8005\u7684\u7ec6\u80de\u4e2d\u4ea7\u751f\u3002\u8fd9\u6837\u5c31\u5728\u4eba\u4f53\u5185\u5927\u91cf\u8868\u8fbe\u4eba\u7279\u5f02\u6027\u7ffb\u8bd1\u540e\u4fee\u9970\u7684\u6297\u539f\u3002RNA\u5206\u5b50\u6216\u75ab\u82d7\u7684\u5927\u89c4\u6a21\u751f\u4ea7\u662f\u672a\u77e5\u7684\u5e76\u4e14\u53ef\u80fd\u5e26\u6765\u6311\u6218\u3002<\/span><\/p>\n<p style=\"text-indent: 32px;line-height: 150%;\"><span style=\"line-height: 150%;font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">\u76ee\u524d\u5c1a\u65e0\u76d1\u7ba1\u673a\u6784\u6279\u51c6\u7684RNA\u75ab\u82d7\uff0c\u4f46\u662f\u7528\u4e8e\u8c03\u8282\u86cb\u767d\u8d28\u7684\u8868\u8fbe\u7684RNA\u53cd\u4e49\u5be1\u6838\u82f7\u9178\u548c\u9002\u4f53\u6cbb\u7597\u5242\u5df2\u7ecf\u83b7\u5f97\u4e34\u5e8a\u6279\u51c6\u3002\u4e5f\u5f00\u53d1\u4e86\u77ed\u5e72\u6270RNA\uff08siRNA\uff09\u6cbb\u7597\u5242\u3002\u8fd9\u4e9bRNA\u7597\u6cd5\u7684\u957f\u5ea6\u6bd4RNA\u75ab\u82d7\u77ed\u5f97\u591a\uff0c\u4f46\u8fd9\u4e3a\u4e34\u5e8a\u6279\u51c6RNA\u75ab\u82d7\u5f00\u8f9f\u4e86\u9053\u8def\u3002\u5f53\u4f7f\u7528\u81ea\u6211\u590d\u5236\u7684RNA\u8f7d\u4f53\u4ee3\u66ff\u57fa\u4e8emRNA\u7684\u75ab\u82d7\u65f6\uff0c\u53ef\u4ee5\u63d0\u9ad8RNA\u75ab\u82d7\u7684\u6548\u7387\u3002\u56e0\u6b64\uff0c\u76ee\u524d\u7684\u7814\u7a76\u96c6\u4e2d\u5728\u4f7f\u7528\u57fa\u4e8eRNA\u4f9d\u8d56\u6027RNA\u805a\u5408\u9176\uff08\u53c8\u540d\u590d\u5236\u9176\uff09\u7684RNA\u75ab\u82d7\u3002\u8fd9\u4e9b\u57fa\u4e8e\u590d\u5236\u9176\u7684RNA\u75ab\u82d7\uff0c\u9996\u5148\u8868\u8fbe\u590d\u5236\u9176\uff0c\u4f7fRNA\u6a21\u677f\u6269\u589e\u3002\u7531\u4e8e\u8f83\u4f4e\u7684\u6210\u672c\uff0c\u6613\u4e8e\u5927\u89c4\u6a21\u751f\u4ea7\u548c\u6709\u5f85\u6539\u8fdb\u7684\u5b89\u5168\u6027\u6f5c\u529b\uff0c\u975e\u75c5\u6bd2\u6295\u9012\u65b9\u6cd5\uff08\u4f8b\u5982\u7eb3\u7c73\u5fae\u7c92\u805a\u5408\u805a\u4e59\u70ef\u4e9a\u80fa\uff08PEI\uff09\uff09\u4f18\u9009\u7528\u4e8eRNA\u75ab\u82d7\u3002<\/span><\/p>\n<p style=\"text-indent: 32px;line-height: 150%;\"><span style=\"line-height: 150%;font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">\u5b9e\u9645\u4e0a\uff0c\u901a\u8fc7\u4f7f\u7528\u7eb3\u7c73\u9897\u7c92\u9633\u79bb\u5b50\u805a\u5408\u590d\u5408\u7269\u6216\u8102\u8d28\u4f53\u5236\u5242\u5c06RNA\u75ab\u82d7\u6295\u9012\u5230\u7ec6\u80de\u8d28\u4e2d\u3002RNA\u7684\u5168\u5408\u6210\u751f\u4ea7\u548c\u6613\u4e8e\u751f\u4ea7\u53ef\u4ee5\u5728\u65b0\u51fa\u73b0\u7684\u75c5\u539f\u4f53\u9274\u5b9a\u540e\u6570\u5468\u5185\u751f\u4ea7\u6570\u5343\u5242\u3002\u9488\u5bf9\u83cc\u682a\u591a\u6837\u6027\u6216\u591a\u4e2a\u611f\u67d3\u6027\u75be\u75c5\u9776\u70b9\u7684\u6784\u5efa\u53ef\u4ee5\u5f88\u5bb9\u6613\u5730\u7ed3\u5408\u3002\u6b64\u5916\uff0c\u8f83\u4f4e\u7684\u57fa\u7840\u8bbe\u65bd\u548c\u8bbe\u5907\u6210\u672c\u4f7f\u5f97\u5728\u4f4e\u6536\u5165\u73af\u5883\u4e2d\u5efa\u7acb\u4ea7\u4e1a\u5316\u6210\u4e3a\u53ef\u80fd\u3002\u56e0\u6b64\uff0c\u4f5c\u8005\u9009\u62e9\u81ea\u6211\u590d\u5236\u7684RNA\u75ab\u82d7\u751f\u4ea7\u4f5c\u4e3a\u8fdb\u4e00\u6b65\u5206\u6790\u7684\u5bf9\u8c61\uff0c\u4e0eDNA\u75ab\u82d7\u76f8\u6bd4\uff0c\u81ea\u6211\u590d\u5236\u7684RNA\u75ab\u82d7\u6613\u4e8e\u4f7f\u7528\u591a\u805a\u7269\u6216\u8102\u8d28\u4f53\u8fdb\u884c\u6295\u9012\uff0c\u4e14\u5177\u6709\u6709\u6548\u6027\u548c\u975e\u7a81\u53d8\u6027\u3002\u6240\u63d0\u51fa\u7684RNA\u75ab\u82d7\u7684\u6d3b\u6027\u836f\u7269\u6210\u5206\u662f\u57fa\u4e8e\u03b1\u75c5\u6bd2\u57fa\u56e0\u7ec4\u7684\u81ea\u6211\u590d\u5236RNA\u5206\u5b50\uff08aka RNA\u590d\u5236\u5b50\uff09\u3002\u8be5RNA\u5206\u5b50\u957f\u5ea6\u4e3a9000-12000\u4e2a\u6838\u82f7\u9178\uff0c\u5e76\u7f16\u7801\u4e86\uff1a1\uff09\u975e\u7ed3\u6784\u86cb\u767d\uff0c\u5176\u81ea\u6211\u88c2\u89e3\u4ee5\u91ca\u653e\u590d\u5236\u5b50RNA\u590d\u5236\u6240\u9700\u7684\u590d\u5236\u9176\u86cb\u767d\u548c\u89e3\u65cb\u9176\u548c2\uff09\u76ee\u7684\u6297\u539f\u57fa\u56e0\u3002\u5728\u8fd9\u4e9b\u7f16\u7801\u57fa\u56e0\u7684\u4fa7\u7ffc\u533a\uff0c\u590d\u5236\u5b50RNA\u8fd8\u542b\u67095&#8217;\u975e\u7ffb\u8bd1\u533a\uff08UTR\uff09\u30013&#8217;UTR\u30015&#8217;\u52a0\u5e3d\u5e8f\u5217\u548cploy\uff08A\uff09\u5c3e\u5e8f\u5217\u3002\u5728\u4f53\u5916\u8f6c\u5f55\u53cd\u5e94\uff08IVT\uff09\u4f7f\u7528\u65e0\u7ec6\u80de\u4ea7\u751f\u81ea\u6211\u590d\u5236\u7684RNA\u5206\u5b50\uff0c\u5982\u56fe3B\u6240\u793a\u3002<\/span><\/p>\n<p style=\"line-height: 150%;\"><strong><span style=\"line-height: 150%;font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">6.1\uff0e\u4e0a\u6e38\u548c\u4e2d\u6e38\u52a0\u5de5<\/span><\/strong><\/p>\n<p style=\"text-indent: 32px;line-height: 150%;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><span style=\"line-height: 150%;\">\u9996\u5148\uff0c\u901a\u8fc7\u5c06\u6297\u539f\u7f16\u7801\u57fa\u56e0\u63d2\u5165RNA\u590d\u5236\u5b50\u8868\u8fbe\u5e8f\u5217\u7684\u6b63\u786e\u4f4d\u7f6e\uff0c\u4ea7\u751f\u7f16\u7801\u5168\u957fRNA\u590d\u5236\u5b50\u7684\u8d28\u7c92DNA\u3002\u7531\u4e8eT7\u542f\u52a8\u5b50\u63a7\u5236RNA\u590d\u5236\u5b50\uff0c\u56e0\u6b64\u53ef\u4f7f\u7528T7 RNA\u805a\u5408\u9176\u8fdb\u884c\u8f6c\u5f55\u3002<\/span> <span style=\"line-height: 150%;\">\u8d28\u7c92DNA\u5728\u5927\u80a0\u6746\u83cc\u4e2d\u6269\u589e\uff0c\u7528\u4e24\u79cd\u9650\u5236\u9176\u7eaf\u5316\u548c\u7ebf\u6027\u5316\u3002RNA\u590d\u5236\u5b50\u76845&#8217;\u672b\u7aef\u53ef\u4ee5\u5728IVT\u671f\u95f4\u901a\u8fc7\u7528m7GpppG\u5e3d\u7ed3\u6784\u7c7b\u4f3c\u7269\u8865\u5145\u53cd\u5e94\u5e76\u4e14\u901a\u8fc7\u4fdd\u6301\u5e3d\u5206\u5b50\u7684\u6469\u5c14\u6bd4\u76f8\u5bf9\u4e8eRNA\u5e8f\u5217\u7684\u7b2c\u4e00\u6838\u82f7\u9178\uff08\u5373GTP\uff09\u9ad8\u6765\u52a0\u5e3d\u3002\u6216\u8005\uff0cRNA\u53ef\u4ee5\u7528\u725b\u75d8\u75c5\u6bd2\u884d\u751f\u7684\u5c01\u76d6\u9176\u5728\u5355\u72ec\u7684\u6b65\u9aa4\u4e2d\u52a0\u76d6\u3002\u5728\u8fd9\u4e2a\u6b65\u9aa4\uff0c\u5355\u4e00IVT\u53cd\u5e94\u4e2d\u4f7f\u7528\u6602\u8d35\u7684\u5e3d\u7c7b\u4f3c\u7269\u4e0e\u4f7f\u7528\u9700\u8981\u5f15\u5165\u989d\u5916\u9176\u4fc3\u5236\u9020\u6b65\u9aa4\u7684\u66f4\u4fbf\u5b9c\u7684\u5c01\u7aef\u53cd\u5e94\u4e4b\u95f4\u51fa\u73b0\u6f5c\u5728\u7684\u6210\u672c\u6743\u8861\u3002<\/span><\/span><\/p>\n<p style=\"text-indent: 32px;line-height: 150%;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><span style=\"line-height: 150%;\">RNA\u75ab\u82d7\u4e2d5&#8217;\u5e3d\u7684\u4f5c\u7528\u662f\uff1a1\uff09\u9632\u6b62\u5916\u5207\u6838\u9178\u9176\u964d\u89e3; 2\uff09\u4fc3\u8fdb\u7ffb\u8bd1; 3\uff09\u907f\u514d\u9488\u5bf9RNA\u5206\u5b50\u7684\u56fa\u6709\u514d\u75ab\u5e94\u7b54\u3002\u901a\u8fc7\u5728DNA\u6a21\u677f\u4e0a\u7f16\u7801poly\uff08A\uff09\u5c3e\u5df4\uff0c\u53ef\u4ee5\u5728IVT\u671f\u95f4\u5bf9RNA\u590d\u5236\u5b50\u76843&#8217;\u672b\u7aef\u8fdb\u884c\u591a\u817a\u82f7\u9178\u5316\u3002<\/span><sup><span style=\"line-height: 150%;font-size: 14px;\">[92]<\/span><\/sup> <span style=\"line-height: 150%;\">\u5c06DNA\u9176I\u9176\u52a0\u5165\u5230IVT\u53cd\u5e94\u6df7\u5408\u7269\u4e2d\u4ee5\u6d88\u5316\u6a21\u677fDNA\u5206\u5b50\u3002<\/span><\/span><\/p>\n<p style=\"line-height: 150%;text-indent: 0em;\"><strong style=\"caret-color: red;\"><span style=\"line-height: 150%;font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">6.2. \u5206\u79bb\u548c\u7eaf\u5316<\/span><\/strong><\/p>\n<p style=\"text-indent: 32px;line-height: 150%;\"><span style=\"line-height: 150%;font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">\u590d\u5236\u5b50RNA\u5206\u5b50\u53ef\u4ee5\u4f7f\u7528TFF\u57fa\u4e8e\u5206\u5b50\u5927\u5c0f\u7684\u5dee\u5f02\u4ece\u53cd\u5e94\u6df7\u5408\u7269\u4e2d\u5206\u79bb\uff0c\u4f7f\u7528\u805a\u781c\u3001\u805a\u919a\u781c\u548c\u805a\u919a\u781c\u6ee4\u819c\u4fee\u9970\u4ee5\u589e\u52a0\u4eb2\u6c34\u6027\u3002 <\/span><\/p>\n<p style=\"text-indent: 32px;line-height: 150%;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><span style=\"line-height: 150%;\">\u8f83\u5c0f\u5206\u5b50\u91cf\u7684\u6742\u8d28\uff08\u4f8b\u5982\uff0c\u6838\u7cd6\u6838\u82f7\u4e09\u78f7\u9178\uff0c\u5c0f\u6838\u9178\u7247\u6bb5\uff0c\u6c28\u57fa\u9178\u7b49\uff09\u901a\u8fc7\u8be5\u819c\u3002 TFF\u671f\u95f4\u7684\u8de8\u819c\u538b\u529b\u901a\u5e38\u7ea6\u4e3a2 \u78c5\/\u5e73\u65b9\u82f1\u5bf8\uff08psi\uff09\uff0c13790 Pa\uff0c\u526a\u5207\u901f\u7387\u7ea6\u4e3a800 <span style=\"line-height: 150%;\">s<sup>-1<\/sup><\/span>\u3002\u5728TFF\u671f\u95f4\uff0c\u8fd8\u53ef\u4ee5\u901a\u8fc7\u5c06\u65b0\u7f13\u51b2\u6db2\u6d41\u5165TFF\u4e2d\u7a7a\u7ea4\u7ef4\u8fc7\u6ee4\u5668\u6765\u8fdb\u884c\u7f13\u51b2\u6db2\u4ea4\u6362\uff08\u900f\u6790\uff09\u3002<\/span> <span style=\"line-height: 150%;\">\u7f13\u51b2\u6db2\u901a\u5e38\u7528\u7ea6PH=8\u7684\u7f13\u51b2\u6db2\u4f53\u4ee3\u66ff\uff0c\u603b\u76d0\u6d53\u5ea6\u7ea6\u4e3a250mM\u3002TFF\u8fc7\u7a0b\u4e2d\u53cd\u5e94\u6df7\u5408\u7269\u7684\u603b\u4f53\u79ef\u589e\u52a05~10\u500d\uff0c\u6700\u7ec8\u4f53\u79ef\u4e0e\u521d\u59cb\u4f53\u79ef\u4e4b\u6bd4\u7ea6\u4e3a8:1\u662f\u8f83\u597d\u7684\u6bd4\u4f8b\u3002<\/span><\/span><\/p>\n<p style=\"text-indent: 32px;line-height: 150%;\"><br  \/><\/p>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section class=\"_135editor\" style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"text-align:center;width: auto;\">\n<section style=\"display:inline-block;clear: both;\">\n<section style=\"padding: 6px 20px;background-image: url('http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/7-1558083948.jpeg');background-repeat: no-repeat;background-size: 100% 100%;letter-spacing: 1.5px;box-sizing: border-box;\">\n<section class=\"135brush\"  style=\"color: rgb(255, 255, 255);\">7. \u8ba8\u8bba\u4e0e\u6280\u672f\u6bd4\u8f83<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 32px;line-height: 150%;\"><span style=\"line-height: 150%;font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">\u4f20\u7edf\u7684\u75ab\u82d7\u751f\u4ea7\u6280\u672f\u4ec5\u9650\u4e8e\u751f\u4ea7\u4e00\u79cd\u75ab\u82d7\u6216\u975e\u5e38\u72ed\u7a84\u7684\u75ab\u82d7\uff0c\u56e0\u6b64\u9700\u8981\u4e3a\u6bcf\u79cd\u75ab\u82d7\u5f00\u53d1\u5355\u72ec\u7684\u751f\u4ea7\u5de5\u827a\uff0c\u4f7f\u5f97\u75ab\u82d7\u751f\u4ea7\u5de5\u827a\u5f00\u53d1\u6210\u672c\u9ad8\u4e14\u8017\u65f6\u3002\u4f46\u662f\uff0c\u53ef\u4ee5\u901a\u8fc7\u5b89\u88c5\u4e00\u6b21\u6027\u4f7f\u7528\u7684\u751f\u4ea7\u8bbe\u5907\uff0c\u53ef\u4ee5\u589e\u52a0\u7075\u6d3b\u6027\u3001\u964d\u4f4e\u6210\u672c\u3001\u5efa\u8bbe\u65f6\u95f4\u548c\u76d1\u7ba1\u98ce\u9669\u3002\u6b64\u5916\uff0c\u5728\u75ab\u82d7\u9700\u6c42\u6700\u9ad8\u7684\u4f4e\u6536\u5165\u6216\u4e2d\u7b49\u6536\u5165\u56fd\u5bb6\u5efa\u8bbe\u751f\u4ea7\u8bbe\u65bd\u5177\u6709\u4ee5\u4e0b\u4f18\u52bf\uff1a\u8f83\u4f4e\u7684\u623f\u5730\u4ea7\u6210\u672c\uff0c\u8f83\u4f4e\u7684\u5efa\u7b51\u6750\u6599\u548c\u5efa\u7b51\u52b3\u52a8\u529b\u6210\u672c\uff0c\u8f83\u4f4e\u7684\u4f4e\u6280\u80fd\u52b3\u52a8\u529b\u6210\u672c\uff0c\u8f83\u4f4e\u7684\u5c06\u75ab\u82d7\u4ea7\u54c1\u8fd0\u8f93\u5230\u9700\u8981\u75ab\u82d7\u5730\u533a\u7684\u8fd0\u8f93\u6210\u672c\u3002\u4f46\u662f\uff0c\u8fd9\u53ef\u80fd\u9700\u8981\u4ece\u53d1\u8fbe\u56fd\u5bb6\u8fdb\u53e3\u4e13\u4e1a\u8bbe\u5907\uff0c\u539f\u6750\u6599\u548c\u5f15\u8fdb\u9ad8\u6280\u672f\u4eba\u624d\u3002\u6b64\u5916\uff0c\u75ab\u82d7\u7684\u6536\u5165\u5f88\u4f4e\uff0c\u539f\u56e0\u5982\u4e0b:a)\u53d1\u5c55\u4e2d\u56fd\u5bb6\u7528\u4e8e\u652f\u4ed8\u75ab\u82d7\u63a5\u79cd\u6210\u672c\u7684\u8d44\u91d1\u4e0d\u8db3;b)\u5728\u53ef\u4ee5\u63d0\u4f9b\u75ab\u82d7\u65f6\uff0c\u75be\u75c5\u7684\u6d41\u884c\u5df2\u7ecf\u6d88\u5931\u3002<\/span><\/p>\n<p style=\"text-indent: 32px;line-height: 150%;\"><span style=\"line-height: 150%;font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">\u4e0a\u8ff0\u7ae0\u8282\u63cf\u8ff0\u7684\u56db\u79cd\u65b0\u5e73\u53f0\u6280\u672f\u53ef\u4ee5\u4f7f\u7528\u4e0a\u8ff0\u6210\u672c\u524a\u51cf\u7b56\u7565\u5b9e\u73b0\u9ad8\u6210\u672c\u6548\u76ca\u3002\u66f4\u91cd\u8981\u7684\u662f\uff0c\u8fd9\u4e9b\u6280\u672f\u53ef\u4ee5\u4f4e\u6210\u672c\u8fd0\u884c\uff0c\u5e76\u4e14\u7075\u6d3b\u7684\u751f\u4ea7\u591a\u79cd\u75ab\u82d7\uff0c\u5b83\u4eec\u53ef\u4ee5\u7528\u4e8e:a) 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150%;font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">\u4e3a\u4e86\u4f30\u8ba1\u8fd9\u56db\u79cd\u65b0\u5174\u75ab\u82d7\u751f\u4ea7\u6280\u672f\u7684\u603b\u4f53\u98ce\u9669\u548c\u53ef\u884c\u6027\uff0c\u5f00\u53d1\u4e86\u4ee5\u4e0b\u516d\u4e2a\u6307\u6807\u5e76\u5e94\u7528\u4e8e\u8fd9\u4e9b\u6280\u672f\uff1a1\uff09\u6280\u672f\u51c6\u5907; 2\uff09\u6280\u672f\u590d\u6742\u6027; 3\uff09\u6a2a\u5411\u6216\u7eb5\u5411\u62d3\u5c55\u7684\u53ef\u884c\u60274\uff09\u7075\u6d3b\u6027\uff0c\u666e\u904d\u9002\u7528\u4e8e\u5236\u9020\u5404\u79cd\u75ab\u82d7; 5\uff09\u75ab\u82d7\u4ea7\u54c1\u5728\u70ed\u5e26\u73af\u5883\u6e29\u5ea6\u4e0b\u7684\u7a33\u5b9a\u6027; 6\uff09\u4ece\u65b0\u5a01\u80c1\u6297\u539f\u7684\u6838\u9178\u5e8f\u5217\u9274\u5b9a\u5230\u75ab\u82d7\u90e8\u7f72\u7684\u54cd\u5e94\u901f\u5ea6\u3002\u5bf9\u4e8e\u6bcf\u4e2a\u8fd9\u516d\u4e2a\u6307\u6807,\u6709 1(\u8868\u793a\u5177\u6709\u6311\u6218\u6027\u6216\u9ad8\u98ce\u9669)\u52305(\u6307\u793a\u53ef\u8fbe\u52305\u5e74\u7684\u7814\u53d1)\u7684\u8bc4\u7ea7\uff0c\u8fd9\u79cd\u8bc4\u7ea7\u57fa\u4e8e\u5bf9\u6587\u732e\u4fe1\u606f\u548c\u5728\u75ab\u82d7\u5b66\uff0c\u5206\u5b50\u548c\u7ec6\u80de\u751f\u7269\u5b66\u7684\u6709\u4e13\u4e1a\u77e5\u8bc6\u7684\u4f5c\u8005\u4ee5\u53ca\u751f\u4ea7\u5de5\u827a\u7684\u53d1\u5c55\u3002\u98ce\u9669\u548c\u53ef\u884c\u6027\u8bc4\u4f30\u7684\u7ed3\u679c\u5982\u4e0b\u88682\u6240\u793a\u3002<\/span><\/p>\n<p style=\"text-indent: 32px;line-height: 150%;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><span style=\"line-height: 150%;\">\u4ece\u6280\u672f\u51c6\u5907\u89d2\u5ea6\u8bc4\u4ef7\uff0c\u81ea\u4ece\u76d1\u7ba1\u673a\u6784\u6279\u51c6\u7684OMV\u75ab\u82d7\u51fa\u73b0\u4ee5\u6765\uff0c\u6700\u9ad8\u7b49\u7ea7\u76845\u4e2a\u5206\u914d\u7ed9GMMA\u75ab\u82d7\uff0c\u7b26\u5408GMP\u8981\u6c42\u7684GMMA\u4ea7\u91cf\u5df2\u7ecf\u5728\u8bd5\u9a8c\u89c4\u6a21\u4e0a\u8fdb\u884c\u4e86\u751f\u4ea7\uff0cGMMA\u75ab\u82d7\u5df2\u7ecf\u901a\u8fc7\u4e86\u7b2c1\u671f\uff08NCT03089879\uff09\u548c\u7b2c2a\u671f\uff08NCT02676895\uff09\uff0c\u73b0\u5728\u6b63\u8fdb\u51652\u671f\u4e34\u5e8a\u8bd5\u9a8c\uff08NCT03527173\uff09\u3002\u56e0\u6b64\uff0c\u8be5\u6280\u672f\u4f3c\u4e4e\u8db3\u591f\u6210\u719f\uff0c\u53ef\u57285\u5e74\u5185\u5b9e\u73b0\u751f\u4ea7\u89c4\u6a21\u5316\u3002\u968f\u7740RNA\u75ab\u82d7\u5df2\u8fbe\u52302\u671f\u548c3\u671f\u4e34\u5e8a\u8bd5\u9a8c\u5e76\u5df2\u5728\u4e2d\u8bd5\u9636\u6bb5\u751f\u4ea7\uff0cRNA\u5e73\u53f0\u5df2\u8fbe\u52304\u7ea7\u3002<\/span> <span style=\"line-height: 150%;\">\u4e3a\u4e86\u6280\u672f\u51c6\u5907\uff0c\u9175\u6bcd\u5e73\u53f0\u7684\u8bc4\u7ea7\u4e3a2\u7ea7\uff0c\u5c3d\u7ba1\u5148\u524d\u5df2\u7ecf\u62a5\u9053\u4e86\u9175\u6bcd\u4e2d\u7684\u4eba\u7cd6\u57fa\u5316\uff0c\u4f46\u4eba\u7c7b\u7cd6\u57fa\u5316\u5c1a\u672a\u6210\u529f\u5b9e\u65bd\u7528\u4e8e\u9175\u6bcd\u4e2d\u7684\u4eba\u6e90\u5316\u86cb\u767d\u8d28\u751f\u7269\u5236\u5242\u3002\u5c3d\u7ba1\u57fa\u4e8e\u9175\u6bcd\u7684\u751f\u4ea7\u89c4\u6a21\u53d1\u9175\u6280\u672f\u5df2\u7ecf\u5f88\u6210\u719f\uff0c\u4f46\u4f7f\u7528\u8be5\u9175\u6bcd\u5e73\u53f0\u7684\u75ab\u82d7\u5f00\u53d1\u4ecd\u5904\u4e8e\u7814\u53d1\u9636\u6bb5\u3002ADDomer\u5e73\u53f0\u7684\u8bc4\u7ea7\u4e3a3\u3002\u867d\u7136ADDomer\u591a\u86cb\u767d\u4ec5\u5728\u5b9e\u9a8c\u5ba4\u89c4\u6a21\u751f\u4ea7\uff0c\u800c\u57fa\u4e8eADDomer\u7684\u75ab\u82d7\u6b63\u5904\u4e8e\u4e34\u5e8a\u524d\u5f00\u53d1\u9636\u6bb5\uff0c\u6606\u866b\u7ec6\u80de &#8211; 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ADDomer\u5e73\u53f0\u8bc4\u4e3a1\u7ea7\uff0c\u5e76\u4e14GMMA\u5e73\u53f0\u8bc4\u7ea7\u4e3a5\uff0c\u56e0\u4e3a\u5b83\u662f\u5177\u6709\u7b80\u5355\u4e0b\u6e38\u7eaf\u5316\u7684\u7ec6\u83cc\u5236\u9020\u6280\u672f\u3002\u9175\u6bcd\u5e73\u53f0\u7684\u6280\u672f\u590d\u6742\u6027\u8bc4\u7ea7\u4e3a3\uff0c\u56e0\u4e3a\u57fa\u4e8e\u9175\u6bcd\u7684\u751f\u4ea7\u662f\u7a33\u5b9a\u7684\uff0c\u4f46\u662f\uff1a.i\uff09\u5b83\u4ecd\u7136\u6613\u53d7\u7ec6\u83cc\u6c61\u67d3; ii\uff09\u4e0b\u6e38\u7eaf\u5316\u76f8\u5bf9\u590d\u6742; iii\uff09\u9ad8\u5ea6\u5de5\u7a0b\u5316\u7684\u9175\u6bcd\u53ef\u80fd\u5bf9\u57f9\u517b\u6761\u4ef6\u7684\u53d8\u5316\u654f\u611f\u3002 RNA\u5e73\u53f0\u7684\u6280\u672f\u590d\u6742\u6027\u4f30\u8ba1\u4e3a2\u7ea7\uff0c\u539f\u56e0\u5728\u4e8e\uff1a1\uff09\u7531\u4e8eRNA\u9176\u6216\u6c34\u89e3\u6c61\u67d3\uff0c\u4e2d\u6e38\u5904\u7406\u548c\u4e0b\u6e38\u7eaf\u5316\u7684\u964d\u89e3\u98ce\u9669\u76f8\u5bf9\u8f83\u9ad8<\/span> <span style=\"line-height: 150%;\">\u548c2\uff09\u7075\u654f\u7684\u9176\u4fc3\u53cd\u5e94\uff0c\u4e2d\u6d41\u8fc7\u7a0b\u548c\u4e0b\u6e38\u7eaf\u5316\u7684\u76f8\u5bf9\u9ad8\u7684\u964d\u89e3\u98ce\u9669\u3002<\/span><\/span><\/p>\n<p style=\"text-indent: 32px;line-height: 150%;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><span style=\"line-height: 150%;\">\u5bf9\u4e8e\u6a2a\u5411\u6216\u7eb5\u5411\u62d3\u5c55\u7684\u53ef\u884c\u6027\uff0cGMMA\u5e73\u53f0\u7684\u8bc4\u7ea7\u4e3a5\uff0c\u56e0\u4e3a\u7ec6\u83cc\u7ec6\u80de\u975e\u5e38\u7a33\u56fa\uff0c\u5e76\u4e14\u751f\u7269\u52a0\u5de5\u548c\u4e0b\u6e38\u52a0\u5de5\u7b80\u5355\u800c\u7a33\u5b9a\u3002<\/span> <span style=\"line-height: 150%;\">\u9175\u6bcd\u5e73\u53f0\u7684\u53ef\u6269\u5c55\u6027\u8bc4\u7ea7\u4e3a4\uff0c\u56e0\u4e3a\u8fd9\u4e9b\u5fae\u751f\u7269\u7684\u7a33\u5b9a\u6027\u5141\u8bb8\u6269\u5927\u89c4\u6a21\uff0c\u4f46\u4e0b\u6e38\u8fc7\u7a0b\u7684\u590d\u6742\u6027\u589e\u52a0\u4e86\u6269\u5927\u89c4\u6a21\u548c\u6269\u5927\u89c4\u6a21\u7684\u96be\u5ea6\u3002RNA\u5e73\u53f0\u56e0\u6613\u4e8e\u6269\u589e\u800c\u8bc4\u7ea7\u4e3a3\uff0c\u56e0\u4e3a\u9176\u4fc3\u53cd\u5e94\u539f\u5219\u4e0a\u662f\u6709\u52a9\u4e8e\u6a2a\u5411\u4e0e\u7eb5\u5411\u7684\u6269\u5c55\uff0c\u800c\u4e14\u4e0b\u6e38\u7eaf\u5316\u4e0d\u50cfGMMA\u6216\u9175\u6bcd\u5e73\u53f0\u590d\u6742\u3002<\/span> <span style=\"line-height: 150%;\">\u7136\u800c\uff0c\u5927\u89c4\u6a21\u53ef\u6301\u7eed\u5236\u9020\u957f\u590d\u5236\u5b50RNA\u7684\u80fd\u529b\u5c1a\u672a\u5f97\u5230\u8bc1\u5b9e\u3002 ADDomer\u5e73\u53f0\u7684\u53ef\u6269\u5c55\u6027\u8bc4\u7ea7\u4e3a3\uff0c\u56e0\u4e3a\u4e0e\u9175\u6bcd\u548c\u7ec6\u83cc\u76f8\u6bd4\uff0c\u6606\u866b\u7ec6\u80de\u5728\u66f4\u9ad8\u7684\u538b\u529b\u548c\u526a\u5207\u901f\u7387\u4e0b\u66f4\u8106\u5f31\uff0c\u56e0\u6b64\u9650\u5236\u4e86\u5176\u6269\u5927\u89c4\u6a21\uff0c\u5e76\u4e14\u7531\u4e8e\u4e0b\u6e38\u8fc7\u7a0b\u7684\u590d\u6742\u6027\u589e\u52a0\u4e86\u6a2a\u5411\u6216\u7eb5\u5411\u6269\u5c55\u7684\u96be\u5ea6\u3002<\/span><\/span><\/p>\n<p style=\"text-indent: 32px;line-height: 150%;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><span style=\"line-height: 150%;\">\u5c31\u7075\u6d3b\u6027\u800c\u8a00\uff0c\u8bc4\u7ea7\u6700\u9ad8\u7684\u6280\u672f\u8bc4\u7ea7\u4e3a5\uff0c\u5728\u7814\u53d1\u76845\u5e74\u5185\uff0c\u53ef\u4ee5\u751f\u4ea7\u4efb\u4f55\u79cd\u7c7b\u7684\u75ab\u82d7\uff0c\u5305\u62ec\uff1a\u5177\u6709\u7c7b\u4f3c\u4eba\u7c7b\u7ffb\u8bd1\u540e\u4fee\u9970\u7684\u86cb\u767d\u8d28\uff08\u590d\u6742\u7684\u7cd6\u57fa\u5316\uff09\uff0c\u5d4c\u5165\u8102\u8d28\u819c\u7684\u86cb\u767d\u8d28\uff0c\u75c5\u6bd2\u8863\u58f3\u578b\u590d\u5408\u7269\u7684\u86cb\u767d\uff0c\u5177\u6709\u7ec6\u83cc\u7279\u5f02\u6027\u7ffb\u8bd1\u540e\u4fee\u9970\u7684\u86cb\u767d\u8d28\u548c\u7ec6\u83cc\u591a\u7cd6\u3002<\/span> <span style=\"line-height: 150%;\">\u7075\u6d3b\u6027\u6700\u4f4e\u4e3a1\u7ea7\uff0c\u8fd9\u662f\u53ea\u751f\u4ea7\u4e00\u79cd\u7279\u5b9a\u75ab\u82d7\u7684\u6280\u672f\uff0c\u5927\u591a\u6570\uff08\u5982\u679c\u4e0d\u662f\u5168\u90e8\uff09\u73b0\u6709\u7684\u75ab\u82d7\u5236\u9020\u6280\u672f\u90fd\u5c5e\u4e8e\u8fd9\u4e00\u7b49\u7ea7\u3002RNA\u5e73\u53f0\u53ef\u4ee5\u7528\u590d\u6742\u7684\u4eba\u7c7b\u7cd6\u57fa\u5316\u8868\u8fbe\u6765\u8868\u8fbe\u4efb\u4f55\u7c7b\u578b\u7684\u86cb\u767d\u8d28\u6297\u539f\uff0c\u7136\u800c\uff0c\u5b83\u4e0d\u80fd\u4ea7\u751f\u7ec6\u83cc\u591a\u7cd6\uff0c\u5e76\u4e14\u7ec6\u83cc\u548c\u5bc4\u751f\u866b\u86cb\u767d\u8d28\u5728\u975e\u5178\u578b\u5730\u52a0\u5de5\uff0c\u56e0\u6b64\u7075\u6d3b\u6027\u7b49\u7ea7\u4e3a4\u7ea7\u3002\u9175\u6bcd\u548cADDomer\u5e73\u53f0\u9650\u5236\u4ea7\u751f\u7ec6\u83cc\u591a\u7cd6\uff0c\u7ec6\u83cc\u7279\u5f02\u6027\u7ffb\u8bd1\u540e\u4fee\u9970\u548c\u8102\u8d28\u819c\u5305\u57cb\u86cb\u767d\uff0c\u56e0\u6b64\u8bc4\u7ea7\u4e3a3\u7ea7\u3002\u4e0e\u4eba\u6e90\u5316\u9175\u6bcd\u8868\u8fbe\u76f8\u53cd\uff0c\u5728\u7ec6\u80de\u5185\u7ffb\u8bd1\u540e\u7cd6\u57fa\u5316\u7684ADDomer\u5206\u5b50\u76ee\u524d\u4e0d\u80fd\u9ad8\u4ea7\u7684\u8868\u8fbe\u3002\u76f8\u53cd\uff0c\u5728\u4eba\u6e90\u5316\u9175\u6bcd\u4e2d\u8868\u8fbe\u7684\u91cd\u7ec4\u86cb\u767d\u53ef\u80fd\u9700\u8981\u7ec4\u88c5\u6210VLPs\u6216\u5d4c\u5165\u819c\u4e2d\uff0c\u4ee5\u8fbe\u5230ADDomer\u5e73\u53f0\u5bb9\u6613\u8bf1\u5bfc\u7684\u514d\u75ab\u539f\u6027\u6c34\u5e73\u3002 GMMA\u5e73\u53f0\u7684\u7075\u6d3b\u6027\u7b49\u7ea7\u4e3a2\u7ea7\uff0c\u56e0\u4e3a\u5b83\u4e0d\u80fd\u4ea7\u751f\u4eba\u7c7b\u7279\u5f02\u6027\u7684\u7ffb\u8bd1\u540e\u4fee\u9970\u548c\u75c5\u6bd2\u8863\u58f3\u6837\u591a\u86cb\u767d\u590d\u5408\u7269\u3002\u5728\u8fd9\u4e9b\u5e73\u53f0\u4e2d\uff0cGMMA\u6700\u9002\u5408\u7ec6\u83cc\u75ab\u82d7\u751f\u4ea7\u3002<\/span><\/span><\/p>\n<p style=\"text-indent: 32px;line-height: 150%;\"><span style=\"line-height: 150%;font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">\u88682. \u7b2c3,4,5\u548c6\u8282\u4e2d\u63cf\u8ff0\u76844\u79cd\u65b0\u5174\u5e73\u53f0\u6280\u672f\u7684\u53ef\u884c\u6027\u548c\u98ce\u9669\u8bc4\u4f30<\/span><\/p>\n<p style=\"text-align: center;\"><img class=\"rich_pages\"          style=\"width: 100%;height: auto;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/1-1558083948.png\" alt=\"\u4f4e\u6210\u672c\u3001\u5feb\u901f\u751f\u4ea7\u75ab\u82d7\u7684\u65b0\u5174\u6280\u672f\" title=\"\u4f4e\u6210\u672c\u3001\u5feb\u901f\u751f\u4ea7\u75ab\u82d7\u7684\u65b0\u5174\u6280\u672f\"  \/><\/p>\n<p style=\"text-indent: 28px;line-height: 150%;\"><span style=\"line-height: 150%;font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">a\uff09\u9175\u6bcd\u5e73\u53f0-\u7528\u4e8e\u91cd\u7ec4\u75ab\u82d7\u751f\u4ea7\u7684\u4eba\u6e90\u5316\u3001\u9ad8\u4ea7\u91cf\u9175\u6bcd\u5e73\u53f0\uff0c\u7528\u4e8e\u91cd\u7ec4\u75ab\u82d7\u751f\u4ea7\u7684ADDomer \u5e73\u53f0-\u6606\u866b\u7ec6\u80de-\u6746\u72b6\u75c5\u6bd2\u5e73\u53f0; GMMA platform-\u5916\u819c\u56ca\u6ce1\u75ab\u82d7\uff0c\u7528\u4e8e\u819c\u6297\u539f\u75ab\u82d7\u751f\u4ea7\u7684\u901a\u7528\u6a21\u5757; RNA\u5e73\u53f0-RNA\u75ab\u82d7\u751f\u4ea7\u3002<\/span><\/p>\n<p style=\"line-height: 150%;\"><span style=\"line-height: 150%;font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">&nbsp;&nbsp; b\uff09\u666e\u904d\u9002\u7528\u4e8e\u5236\u9020\u5404\u79cd\u75ab\u82d7\u3002<\/span><\/p>\n<p style=\"line-height: 150%;\"><span style=\"line-height: 150%;font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">&nbsp;&nbsp; c\uff09\u603b\u4f53\u53ef\u884c\u6027\u548c\u98ce\u9669\u8bc4\u4f30\u662f\u901a\u8fc7\u603b\u8ba1\u6bcf\u9879\u6280\u672f\u7684\u6bcf\u4e2a\u6307\u6807\u503c\u8ba1\u7b97\u7684\u3002<\/span><\/p>\n<p style=\"text-indent: 32px;line-height: 150%;\"><br  \/><\/p>\n<p style=\"text-indent: 32px;line-height: 150%;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><span style=\"line-height: 150%;\">\u5bf9\u4e8e\u70ed\u7a33\u5b9a\u6027\uff0c\u6700\u9ad8\u662f5\u7ea7\uff0c\u8be5\u7b49\u7ea7\u4e0b\u5bf9\u75ab\u82d7\u572840\u2103\u4e0b\u534a\u8870\u671f\u81f3\u5c11\u4e3a6\u4e2a\u6708\uff0c\u7814\u53d1\u6295\u5165\u957f\u8fbe5\u5e74\u3002ADDomer\u53ef\u4ee5\u88ab\u4e8c\u786b\u952e\u7a33\u5b9a\uff0c\u4f7f\u5176\u572837\u2103\u548c45\u2103\u4e4b\u95f4\u7684\u70ed\u7a33\u5b9a\u6027\u589e\u52a0\u6570\u6708\uff0c\u56e0\u6b64\uff0c\u8fd9\u6837\u4f3c\u4e4e\u53ef\u4ee5\u4f7fADDomer\u5e73\u53f0\u662f\u70ed\u7a33\u5b9a\u6027\u8fbe\u52305\u7ea7\u3002GMMA\u5728\u5ba4\u6e29\u4e0b\u7a33\u5b9a\u6570\u5468\uff0c\u56e0\u6b64\uff0c\u5176\u70ed\u7a33\u5b9a\u6027\u4f30\u8ba1\u4e3a3\u7ea7\u3002\u7531\u9175\u6bcd\u5e73\u53f0\u4ea7\u751f\u7684\u91cd\u7ec4\u75ab\u82d7\u5177\u6709\u5e7f\u6cdb\u7684\u70ed\u7a33\u5b9a\u6027\uff0c\u4f46\u662f\u901a\u5e38\u914d\u5236\u5728\u70ed\u7a33\u5b9a\u4ea7\u54c1\u4e2d\uff0c\u56e0\u6b64\u5b83\u4eec\u7684\u70ed\u7a33\u5b9a\u6027\u7ea6\u4e3a3\u7ea7\u3002RNA\u5206\u5b50\u76f8\u5bf9\u6bd4\u8f83\u8106\u5f31\uff0c\u5373\u4f7f\u6ca1\u6709RNA\u9176\uff0c\u4e5f\u53ef\u4ee5\u6c34\u89e3\u6216\u916f\u4ea4\u6362\uff0c\u56e0\u6b64\uff0c\u70ed\u7a33\u5b9a\u6027\u8bc4\u5206\u4e3a2\u7ea7\u3002\u4f46\u662f\u4f7f\u7528\u51bb\u5e72\u6216\u79bb\u5b50\u6db2\u4f53\u4f3c\u4e4e\u53ef\u4ee5\u663e\u7740\u589e\u52a0RNA\u75ab\u82d7\u7684\u70ed\u7a33\u5b9a\u6027\uff0c\u76ee\u524d\u5df2\u7ecf\u5728DNA\u548csiRNA\u5206\u5b50\u4e0a\u5b9e\u73b0\u3002<\/span> <span style=\"line-height: 150%;\">\u51bb\u5e72\u548c\u79bb\u5b50\u6db2\u4f53\u4e5f\u53ef\u4ee5\u589e\u52a0\u6240\u63d0\u53ca\u7684\u5176\u4ed6\u75ab\u82d7\u7684\u70ed\u7a33\u5b9a\u6027\u3002\u5728\u6ca1\u6709\u51b7\u94fe\u7684\u60c5\u51b5\u4e0b\uff0c\u70ed\u7a33\u5b9a\u6027\u5bf9\u4e8e\u5904\u4e8e\u70ed\u5e26\u5730\u533a\u3001\u4ea4\u901a\u504f\u8fdc\u793e\u533a\u7684\u6700\u540e\u7684\u4ea4\u4ed8\u8fc7\u7a0b\u5c24\u5176\u91cd\u8981\u3002<\/span><\/span><\/p>\n<p style=\"text-indent: 32px;line-height: 150%;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><span style=\"line-height: 150%;\">\u5173\u4e8e\u54cd\u5e94\u901f\u5ea6\uff0cRNA\u5e73\u53f0\u7684\u8bc4\u7ea7\u4e3a5\u7ea7\uff0c\u8fd9\u662f\u56e0\u4e3a\u751f\u6210\u7279\u5b9aDNA\u6a21\u677f\u548cRNA\u590d\u5236\u5b50\u5e8f\u5217\u7684\u7b80\u4fbf\u6027\u548c\u901f\u5ea6\u3002<\/span> <span style=\"line-height: 150%;\">\u7531\u4e8eMultiBac\u7cfb\u7edf\u53ef\u63d0\u4f9b\u7684\u5feb\u901f\u9057\u4f20\u8bbe\u8ba1\uff0c\u6240\u4ee5ADDomer\u5e73\u53f0\u7684\u8bc4\u7ea7\u4e3a4\u7ea7\u3002 OMV\u7684\u9057\u4f20\u91cd\u65b0\u8bbe\u8ba1\u975e\u5e38\u8017\u65f6\uff0c\u56e0\u6b64GMMA\u5e73\u53f0\u7684\u54cd\u5e94\u901f\u5ea6\u4e3a2\u7ea7\u3002<\/span> <span style=\"line-height: 150%;\">\u9175\u6bcd\u5e73\u53f0\u7684\u8bc4\u7ea7\u4e3a1\u7ea7\uff0c\u56e0\u4e3a\u5b83\u9700\u8981\u5148\u8fdb\u7684\u9057\u4f20\u4fe1\u606f\u6539\u9020\u6280\u672f\u4ee5\u4ea7\u751f\u9ad8\u4ea7\u91cf\u7684\u5206\u6ccc\u7ec6\u80de\u7cfb\u3002<\/span><\/span><\/p>\n<p style=\"text-indent: 32px;line-height: 150%;\"><span style=\"line-height: 150%;font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">\u6700\u540e\uff0c\u901a\u8fc7\u5bf9\u88682\u4e2d\u5404\u5217\u7684\u6bcf\u79cd\u6280\u672f\u6bcf\u4e2a\u5ea6\u91cf\u503c\u8fdb\u884c\u6c42\u548c\uff0c\u8ba1\u7b97\u51fa\u7edf\u4e00\u52a0\u6743\u7684\u603b\u4f53\u53ef\u884c\u6027\u548c\u98ce\u9669\u8bc4\u4f30\u3002\u8fd9\u6837\uff0c\u6309\u8fd1\u671f\u53ef\u884c\u6027\u548c\u964d\u4f4e\u98ce\u9669\u7684\u987a\u5e8f\u7684\u6280\u672f\u662f\uff1a1\uff09\u9175\u6bcd\u5e73\u53f0;2\uff09ADDomer\u5e73\u53f0; 3\uff09RNA\u5e73\u53f0; 4\uff09GMMA\u5e73\u53f0\u3002\u8fd9\u6837\u5bf9\u6bd4\u8f83\u6982\u8ff0\u6709\u52a9\u4e8e\u7814\u7a76\u4f18\u5148\u7ea7\u5212\u5206\uff0c\u4ee5\u8fdb\u4e00\u6b65\u6539\u8fdb\u8fd9\u4e9b\u5e73\u53f0\u7684\u6280\u672f\u3002<\/span><\/p>\n<p style=\"text-indent: 32px;line-height: 150%;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\"><span style=\"line-height: 150%;\">&nbsp;\u4f5c\u4e3a\u5b9e\u73b0\u5168\u9762\u5546\u4e1a\u5316\u7684\u89c4\u5212\u56fe\u7684\u4e00\u90e8\u5206\uff0c\u5e94\u901a\u8fc7\u5efa\u6a21\uff0c\u6a21\u62df\u548c\u4f18\u5316\u751f\u4ea7\u8fc7\u7a0b\u53ca\u5176\u5404\u81ea\u7684\u4f9b\u5e94\u94fe\u6765\u8be6\u7ec6\u8bc4\u4f30\u8fd9\u4e9b\u75ab\u82d7\u5e73\u53f0\u6280\u672f\u7684\u6280\u672f\u548c\u7ecf\u6d4e\u53ef\u884c\u6027\u3002\u56e0\u6b64\uff0c\u9664\u4e86\u4e0a\u8ff0\u516d\u4e2a\u6307\u6807\u5916\uff0c\u8d44\u91d1\u6210\u672c\uff08\u4e3b\u8981\u5305\u62ec\u8bbe\u65bd\u5efa\u8bbe\uff0c\u8bbe\u5907\u548c\u673a\u68b0\u6210\u672c\uff09\u548c\u8fd0\u8425\u548c\u7ef4\u62a4\u6210\u672c\uff08\u4e3b\u8981\u5305\u62ec\u539f\u6750\u6599\u548c\u6d88\u8017\u54c1\u7684\u6210\u672c\uff0c\u52b3\u52a8\u529b\u6210\u672c\u548c\u516c\u7528\u4e8b\u4e1a\u6210\u672c\uff09<\/span> <span style=\"line-height: 150%;\">\u4e5f\u5e94\u8be5\u8ba1\u7b97\u8fdb\u53bb\u3002\u5728\u7ecf\u6d4e\u4e0a\uff0c\u5bf9\u4e8e\u6280\u672f\u6700\u53ef\u884c\u7684\u75ab\u82d7\u5e73\u53f0\u6280\u672f\uff0c\u5e94\u8be5\u5efa\u7acb\u4e00\u4e2a\u4e2d\u8bd5\u89c4\u6a21\u7684\u8bbe\u65bd\uff0c\u53ef\u4ee5\u4ee5\u66f4\u4f4e\u5bf9\u6210\u672c\u4e3a\u4e34\u5e8a\u8bd5\u9a8c\u751f\u4ea7\u5404\u79cd\u9ad8\u9700\u6c42\u6216\u76ee\u524d\u6602\u8d35\u7684\u75ab\u82d7\u3002\u4e3a\u4e86\u6ee1\u8db3\u75ab\u82d7\u9700\u6c42\u5e76\u8fdb\u4e00\u6b65\u964d\u4f4e\u751f\u4ea7\u6210\u672c\uff0c\u5e94\u5efa\u7acb\u8d77\u751f\u4ea7\u89c4\u6a21\u8bbe\u65bd\u3002\u5e94\u5c06\u5229\u6da6\u518d\u6295\u8d44\u4e8e\u5efa\u8bbe\u66f4\u591a\u7684\u751f\u4ea7\u89c4\u6a21\u8bbe\u65bd\uff0c\u8fd9\u5c06\u6709\u52a9\u4e8e\u6ee1\u8db3\u533a\u57df\u548c\u56fd\u9645\u75ab\u82d7\u9700\u6c42\u3002<\/span><\/span><\/p>\n<p style=\"line-height: 150%;\"><br  \/><\/p>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section class=\"_135editor\" style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"text-align:center;width: auto;\">\n<section style=\"display:inline-block;clear: both;\">\n<section style=\"padding: 6px 20px;background-image: url('http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/7-1558083948.jpeg');background-repeat: no-repeat;background-size: 100% 100%;letter-spacing: 1.5px;box-sizing: border-box;\">\n<section class=\"135brush\"  style=\"color: rgb(255, 255, 255);\">8. \u7ed3\u8bba<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 28px;line-height: 150%;\"><span style=\"line-height: 150%;font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">\u672c\u6587\u56de\u987e\u4e86\u73b0\u6709\u7684\u75ab\u82d7\u751f\u4ea7\u6280\u672f\uff0c\u5e76\u5bf9\u4ee5\u4e0b\u56db\u79cd\u6709\u524d\u666f\u7684\u75ab\u82d7\u751f\u4ea7\u6280\u672f\u8fdb\u884c\u4e86\u8bc4\u4ef7\uff1a1) \u4eba\u6e90\u5316\uff0c\u9ad8\u4ea7\u91cf\u7684\u9175\u6bcd\u5e73\u53f0\uff1b2) \u6606\u866b\u7ec6\u80de-\u6746\u72b6\u75c5\u6bd2 ADDomer\u5e73\u53f0\uff1b3) GMMA\u75ab\u82d7\u5e73\u53f0\uff1b4) RNA\u75ab\u82d7\u5e73\u53f0\u3002\u8fd9\u56db\u79cd\u6280\u672f\u7684\u4f18\u7f3a\u70b9\u5206\u522b\u901a\u8fc7\u4ee5\u4e0b\u516d\u4e2a\u6307\u6807\u8fdb\u884c\u8bc4\u4f30:\u6280\u672f\u76ee\u524d\u8fdb\u5c55\u3001\u6280\u672f\u590d\u6742\u6027\u3001\u6613\u4e8e\u6269\u5c55\u3001\u7075\u6d3b\u6027\u3001\u4ea7\u54c1\u70ed\u7a33\u5b9a\u6027\u548c\u54cd\u5e94\u901f\u5ea6\u3002\u5728\u6b64\u57fa\u7840\u4e0a\uff0c\u53ef\u4ee5\u5bf9\u8fd9\u4e9b\u75ab\u82d7\u5e73\u53f0\u6280\u672f\u8fdb\u884c\u6539\u8fdb\u3002\u8fd9\u56db\u79cd\u65b0\u5174\u6280\u672f\u7684\u6392\u5217\u5982\u4e0b\uff08\u53ef\u884c\u6027\u4f9d\u6b21\u964d\u4f4e\uff0c\u98ce\u9669\u6027\u4f9d\u6b21\u9012\u589e\uff09\uff1a1) GMMA \u5e73\u53f0\uff1b2) RNA\u5e73\u53f0\uff1b3) ADDomer \u5e73\u53f0\uff1b4) \u9175\u6bcd\u5e73\u53f0\u3002\u4f46\u662f\u4e3a\u4e86\u66f4\u51c6\u786e\u5730\u8bc4\u4f30\u8fd9\u4e9b\u6280\u672f\u7684\u6280\u672f\u7ecf\u6d4e\u53ef\u884c\u6027\uff0c\u5e94\u9488\u5bf9\u6700\u53ef\u884c\u7684\u6280\u672f\u5efa\u9020\u751f\u4ea7\u8bbe\u65bd\uff0c\u518d\u8fdb\u884c\u8fdb\u4e00\u6b65\u63a2\u7d22\u3002<\/span><\/p>\n<p style=\"text-indent: 28px;line-height: 150%;\"><br  \/><\/p>\n<p style=\"line-height: 150%;\"><span style=\"font-size: 14px;font-family: \u5b8b\u4f53, SimSun;\">\u539f\u6587\u6765\u6e90\uff1aKis Z, Shottock R, Shah N, et al. <em>Emerging technologies for low-cost, rapid vaccine manufacture<\/em>. Biotechnolo,J,2018<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;letter-spacing: 0.544px;white-space: normal;font-size: 16px;background-color: rgb(255, 255, 255);font-family: \u5fae\u8f6f\u96c5\u9ed1;line-height: 32px;text-align: center;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 20px;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box;text-indent: 27px;letter-spacing: 0.54px;widows: 1;font-size: 18px;overflow-wrap: break-word !important;\"><span style=\"max-width: 100%;color: rgb(0, 209, 0);box-sizing: border-box !important;overflow-wrap: break-word !important;\">\u57f9\u8bad\u63a8\u8350<\/span><\/strong><\/span><\/p>\n<p style=\"white-space: normal;max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;background-color: rgb(255, 255, 255);font-family: \u5fae\u8f6f\u96c5\u9ed1;line-height: 32px;text-align: center;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MjM5NTI3MDE0NQ==&amp;mid=2653622204&amp;idx=8&amp;sn=557d45528da9fef4115187e3be05efc3&amp;chksm=bd24b4d28a533dc45bbc1762bf8ec6e76306c6557755276531dab9a5d734770ba71efb84303a&amp;scene=21#wechat_redirect\" target=\"_blank\"   style=\"-webkit-tap-highlight-color: rgba(0, 0, 0, 0);max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 15px;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><span style=\"max-width: 100%;-webkit-tap-highlight-color: rgba(0, 0, 0, 0);box-sizing: border-box !important;overflow-wrap: break-word !important;\">\u836f\u4f01\u516c\u7528\u5de5\u7a0b\u8bbe\u8ba1\u3001\u9a8c\u8bc1\u53ca\u65e5\u5e38\u7ba1\u7406\u7ef4\u62a4\uff08\u6b66\u6c49\uff09\u6280\u672f\u4ea4\u6d41\u4f1a<\/span><\/strong><\/span><\/a><br style=\"max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\"  \/><\/p>\n<p style=\"white-space: normal;max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;background-color: rgb(255, 255, 255);font-family: \u5fae\u8f6f\u96c5\u9ed1;line-height: 32px;text-align: center;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MjM5NTI3MDE0NQ==&amp;mid=2653622147&amp;idx=4&amp;sn=1d1a215a0fba530345b894ef7b3883f6&amp;chksm=bd24b4ed8a533dfbf465b5bd88e1c507f3c88ff2e92f0e3d0c9af60add4ad9c79ed55249bf83&amp;scene=21#wechat_redirect\" target=\"_blank\"   style=\"-webkit-tap-highlight-color: rgba(0, 0, 0, 0);max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 15px;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><span style=\"max-width: 100%;-webkit-tap-highlight-color: rgba(0, 0, 0, 0);box-sizing: border-box !important;overflow-wrap: break-word !important;\">\u836f\u4f01\u5982\u4f55\u5f00\u5c55\u4e34\u5e8a\u524d\u5b89\u5168\u6027\u7814\u7a76\u4e13\u9898\u57f9\u8bad\u73ed\uff08ICH S\u6307\u5bfc\uff09<\/span><\/strong><\/span><\/a><br style=\"max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\"  \/><\/p>\n<p style=\"white-space: normal;max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;background-color: rgb(255, 255, 255);font-family: \u5fae\u8f6f\u96c5\u9ed1;line-height: 32px;text-align: center;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MjM5NTI3MDE0NQ==&amp;mid=2653622410&amp;idx=6&amp;sn=7984f227ca630ba102ba0b247574d26b&amp;chksm=bd24abe48a5322f2ee4905ae29c2aac638fa7309c19dfdbd86316a8515a9799f4add113bb2f7&amp;scene=21#wechat_redirect\" target=\"_blank\"   style=\"-webkit-tap-highlight-color: rgba(0, 0, 0, 0);max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\">\u201c\u6570\u636e\u5b8c\u6574\u6027\u53ca\u8ba1\u7b97\u673a\u5316\u7cfb\u7edf\u9a8c\u8bc1\u201d\u6280\u672f\u4ea4\u6d41\u4f1a<\/a><\/strong><\/p>\n<p style=\"white-space: normal;max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;background-color: rgb(255, 255, 255);font-family: \u5fae\u8f6f\u96c5\u9ed1;line-height: 32px;text-align: center;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 15px;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><span style=\"max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzA5MDY4MDQ1NQ==&amp;mid=2649834371&amp;idx=6&amp;sn=58a1eeec283acc3cd2d496b9697c0363&amp;chksm=88021e6bbf75977d8a6ec44b1e420eacbae4290e187bfc82a3702d2eab2df38c3478b732b026&amp;scene=21#wechat_redirect\" target=\"_blank\"   style=\"-webkit-tap-highlight-color: rgba(0, 0, 0, 0);max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\">\u65b0\u6cd5\u89c4\u4e0b\u836f\u54c1\u6ce8\u518c\u7533\u62a5\u7684\u91cd\u70b9\u96be\u70b9\u5206\u6790\u4e13\u9898\u7814\u4fee\u73ed<\/a><\/span><\/strong><\/span><\/p>\n<p style=\"white-space: normal;max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;background-color: rgb(255, 255, 255);font-family: \u5fae\u8f6f\u96c5\u9ed1;line-height: 32px;text-align: center;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 15px;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><span style=\"max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><strong style=\"-webkit-tap-highlight-color: rgba(0, 0, 0, 0);max-width: 100%;font-size: 16px;letter-spacing: 0.544px;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MjM5NTI3MDE0NQ==&amp;mid=2653622410&amp;idx=5&amp;sn=8f89aee1ffe1990e50018ff51c1d3bed&amp;chksm=bd24abe48a5322f22b977a9ef82f85a96ae40ed017d5a1545a71e52f915c927477bc01b8aade&amp;scene=21#wechat_redirect\" target=\"_blank\"   style=\"-webkit-tap-highlight-color: rgba(0, 0, 0, 0);max-width: 100%;font-size: 16px;letter-spacing: 0.544px;box-sizing: border-box !important;overflow-wrap: break-word !important;\">\u57fa\u4e8e\u5927\u6570\u636e\u5b8c\u6574\u6027\u7684\u98ce\u9669\u5e94\u5bf9OOS\/OOT\u8c03\u67e5\u4e0eCAPA\u201d\u6280\u672f\u4ea4\u6d41\u4f1a<\/a><\/strong><\/span><\/strong><\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;white-space: normal;font-family: \u5fae\u8f6f\u96c5\u9ed1;font-size: 16px;background-color: rgb(255, 255, 255);text-align: center;letter-spacing: 0.54px;line-height: normal;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><strong style=\"max-width: 100%;color: rgb(255, 41, 65);line-height: 25.6px;font-size: 18px;box-sizing: border-box !important;overflow-wrap: break-word !important;\">\u8bc6\u522b\u5fae\u4fe1\u4e8c\u7ef4\u7801\uff0c\u6dfb\u52a0\u5c0f\u7f16\uff0c\u7b26\u5408\u6761\u4ef6\u8005\u5373\u53ef\u52a0\u5165\u751f\u7269\u5236\u54c1\u5fae\u4fe1\u7fa4\uff01<\/strong><\/p>\n<section 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100%;letter-spacing: 0.54px;box-sizing: border-box !important;overflow-wrap: break-word !important;\">\n<section label=\"Powered by 135editor.com\"  style=\"max-width: 100%;line-height: 25.6px;box-sizing: border-box !important;overflow-wrap: break-word !important;\">\n<p style=\"max-width: 100%;min-height: 1em;text-align: center;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><img class=\"\"       style=\"box-sizing: border-box !important;overflow-wrap: break-word !important;visibility: visible !important;width: 253px !important;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/2-1558083948.jpeg\" alt=\"\u4f4e\u6210\u672c\u3001\u5feb\u901f\u751f\u4ea7\u75ab\u82d7\u7684\u65b0\u5174\u6280\u672f\" title=\"\u4f4e\u6210\u672c\u3001\u5feb\u901f\u751f\u4ea7\u75ab\u82d7\u7684\u65b0\u5174\u6280\u672f\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;text-align: center;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><img 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!important;\">\n<section style=\"margin-top: 0.5em;margin-bottom: 0.5em;max-width: 100%;text-align: center;box-sizing: border-box !important;overflow-wrap: break-word !important;\">\n<section style=\"margin: -0.9em auto 0.9em;max-width: 100%;width: 0.6em;height: 0.6em;box-sizing: border-box !important;overflow-wrap: break-word !important;\">\n<section style=\"margin-left: 1px;max-width: 100%;border-radius: 100%;width: 9.59px;height: 9.59px;background-color: rgb(170, 33, 94);box-sizing: border-box !important;overflow-wrap: break-word !important;\"><br  style=\"max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\"  \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"max-width: 100%;letter-spacing: 0.544px;white-space: normal;font-family: \u5fae\u8f6f\u96c5\u9ed1;line-height: 25.6px;font-size: 14px;background-color: rgb(255, 255, 255);box-sizing: border-box !important;overflow-wrap: break-word !important;\">\n<section 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border-box !important;overflow-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\">\u58f0<\/section>\n<\/section>\n<section style=\"max-width: 100%;border-radius: 100%;width: 2em;height: 2em;text-align: center;color: rgb(255, 255, 255);line-height: 2em;vertical-align: top;display: inline-block;background-color: rgb(170, 33, 94);box-sizing: border-box !important;overflow-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\">\u660e<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"max-width: 100%;min-height: 1em;letter-spacing: 0.544px;white-space: normal;font-family: \u5fae\u8f6f\u96c5\u9ed1;font-size: 16px;background-color: rgb(255, 255, 255);box-sizing: border-box !important;overflow-wrap: break-word !important;\"><span style=\"max-width: 100%;line-height: 25.6px;font-size: 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100%;min-height: 1em;text-align: center;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><img class=\"\"   width=\"auto\"  style=\"padding: 10px;color: inherit;text-align: justify;line-height: 28.4444px;border-width: 1px;border-style: solid;border-color: rgb(208, 208, 208);box-shadow: rgba(7, 1, 3, 0.4) 2px 2px 5px;box-sizing: border-box !important;overflow-wrap: break-word !important;visibility: visible !important;width: auto !important;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/9-1558083948.jpeg\" alt=\"\u4f4e\u6210\u672c\u3001\u5feb\u901f\u751f\u4ea7\u75ab\u82d7\u7684\u65b0\u5174\u6280\u672f\" title=\"\u4f4e\u6210\u672c\u3001\u5feb\u901f\u751f\u4ea7\u75ab\u82d7\u7684\u65b0\u5174\u6280\u672f\"  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