{"id":106492,"date":"2019-04-03T00:00:50","date_gmt":"2019-05-17T09:10:39","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/106492\/"},"modified":"2019-05-17T17:10:42","modified_gmt":"2019-05-17T09:10:42","slug":"8f925d38f5","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/106492\/","title":{"rendered":"\u4eb2\u548c\u6807\u7b7e\u5728\u91cd\u7ec4\u86cb\u767d\u8868\u8fbe\u4e0e\u7eaf\u5316\u7684\u4f7f\u7528\u53ca\u6bd4\u8f83"},"content":{"rendered":"<div class=\"bpp-post-content\">\n<section  label=\"Powered by 135editor.com\" style=\"font-size:16px;\">\n<p style=\"margin-bottom: 0in;text-indent: 2em;\"><span style=\"font-family: \u5b8b\u4f53, SimSun;font-size: 15px;text-indent: 0.29in;\">\u91cd\u7ec4\u86cb\u767d\u5728\u79d1\u5b66\u7814\u7a76\u548c\u5de5\u4e1a\u751f\u4ea7\u4e2d\u7684\u5e94\u7528\u8d8a\u6765\u8d8a\u591a\uff0c\u4e0d\u4ec5\u5c40\u9650\u4e8e\u666e\u901a\u86cb\u767d\uff0c\u4e5f\u7528\u4e8e\u75c5\u6bd2\u6837\u9897\u7c92\u7b49\u7684\u5236\u5907\u3002\u76ee\u524d\u5df2\u7ecf\u5f62\u6210\u4e86\u591a\u79cd\u8868\u8fbe\u548c\u7eaf\u5316\u7b56\u7565\uff0c\u8fd9\u4e9b\u7b56\u7565\u65e8\u5728\u7ed5\u8fc7\u6216\u7f13\u89e3\u86cb\u767d\u8d28\u6eb6\u89e3\u6027\u5dee\u3001\u5bf9\u5bbf\u4e3b\u7684\u6bd2\u6027\u3001\u4f4e\u6c34\u5e73\u8868\u8fbe\u548c\u7eaf\u5316\u56f0\u96be\u7b49\u95ee\u9898\u3002<\/span><\/p>\n<p style=\"text-indent: 0.29in;margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">\u91cd\u7ec4\u86cb\u767d\u7684\u4eb2\u548c\u7eaf\u5316\u901a\u5e38\u662f\u5c06\u91cd\u7ec4\u86cb\u767d\u514b\u9686\u5230\u542b\u6709\u5bf9\u56fa\u5b9a\u5316\u6811\u8102\u5177\u6709\u9ad8\u5ea6\u7279\u5f02\u6027\u7684\u878d\u5408\u86cb\u767d\u6216\u80bd\u6807\u8bb0\u7684\u8868\u8fbe\u8f7d\u4f53\u4e2d\u3002\u5728\u8bb8\u591a\u60c5\u51b5\u4e0b\uff0c\u878d\u5408\u86cb\u767d\u6216\u80bd\u4e5f\u4f1a\u589e\u52a0\u91cd\u7ec4\u86cb\u767d\u7684\u6eb6\u89e3\u6027\uff0c\u964d\u4f4e\u5bf9\u5bbf\u4e3b\u7ec6\u83cc\u7ec6\u80de\u7684\u6bd2\u6027\u3002\u4f46\u5e26\u6709\u4eb2\u548c\u6807\u7b7e\u7684\u91cd\u7ec4\u86cb\u767d\u4e5f\u9762\u4e34\u4e00\u4e9b\u95ee\u9898\uff0c\u6bd4\u5982\u6807\u7b7e\u6709\u65f6\u4f1a\u5bf9\u76ee\u7684\u86cb\u767d\u7684\u7ed3\u6784\u751a\u81f3\u751f\u7269\u6d3b\u6027\u4ea7\u751f\u6d88\u6781\u5f71\u54cd\uff0c\u8fd9\u5c31\u9700\u8981\u5bf9\u6807\u7b7e\u8fdb\u884c\u53bb\u9664\u3002<\/span><\/p>\n<p style=\"text-indent: 0.29in;margin-bottom: 0in;\"><br  \/><\/p>\n<p style=\"text-align: justify;margin-bottom: 0in;text-indent: 0em;\"><img loading=\"lazy\" align=\"bottom\" border=\"0\" class=\"\"        height=\"202\" hspace=\"1\" style=\"width: 100%;height: auto;\" width=\"601\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/4-1558084240.jpeg\" alt=\"\u4eb2\u548c\u6807\u7b7e\u5728\u91cd\u7ec4\u86cb\u767d\u8868\u8fbe\u4e0e\u7eaf\u5316\u7684\u4f7f\u7528\u53ca\u6bd4\u8f83\" title=\"\u4eb2\u548c\u6807\u7b7e\u5728\u91cd\u7ec4\u86cb\u767d\u8868\u8fbe\u4e0e\u7eaf\u5316\u7684\u4f7f\u7528\u53ca\u6bd4\u8f83\"  \/><\/p>\n<p style=\"margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">\u4eb2\u548c\u8868\u8fbe\u53ca\u9176\u5207\u7b56\u7565<sup>\u3010<\/sup><sup>1\u3011<\/sup><\/span><\/p>\n<p style=\"text-indent: 0.29in;margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">1. \u878d\u5408\u8868\u8fbe\u4e00\u4e2a\u4eb2\u548c\u6807\u7b7e\u53ca\u7528\u4e8e\u7eaf\u5316\u540e\u9176\u5207\u7684\u9176\u5207\u4f4d\u70b9linker<\/span><\/p>\n<p style=\"text-indent: 0.29in;margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">2. \u4eb2\u548c\u6807\u7b7e\u65e2\u662f\u7eaf\u5316\u6807\u7b7e\u540c\u65f6\u4e5f\u4fc3\u8fdb\u76ee\u7684\u86cb\u767d\u7684\u53ef\u6eb6\u6027\u8868\u8fbe<\/span><\/p>\n<p style=\"text-indent: 0.29in;margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">3. \u7528\u4e8e\u5916\u5207\u9176\uff08<span style=\"color: rgb(50, 50, 50);\">&nbsp;TAGZyme<\/span>\uff09\u5728\u7eaf\u5316\u540e\u4eceN\u7aef\u5207\u9664\u4eb2\u548c\u7eaf\u5316\u6807\u7b7e<\/span><\/p>\n<p style=\"text-indent: 0.29in;margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">4.\u90e8\u5206\u4fc3\u8fdb\u53ef\u6eb6\u6027\u8868\u8fbe\u7684\u5e8f\u5217\u4e0d\u5e26\u6709\u4eb2\u548c\u6807\u7b7e\uff0c\u4f8b\u5982Trx\uff0c\u56e0\u6b64\u9700\u8981\u989d\u5916\u52a0\u4e0a\u4eb2\u548c\u6807\u7b7e<\/span><\/p>\n<p style=\"text-align:center;text-indent: 0.29in;margin-bottom: 0in;\"><br  \/><\/p>\n<p style=\"text-indent: 0.29in;margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">\u5728\u672c\u6587\u4e2d\uff0c\u6211\u4eec\u5c06\u603b\u7ed3\u4f7f\u7528\u4eb2\u548c\u6807\u8bb0\u6765\u7f13\u89e3\u86cb\u767d\u8d28\u6eb6\u89e3\u6027\u5bbf\u4e3b\u6bd2\u6027\u3001\u8868\u8fbe\u6c34\u5e73\u53ca\u7eaf\u5316\u95ee\u9898\u8fd9\u7684\u5404\u79cd\u7b56\u7565\uff0c\u4eb2\u548c\u6807\u8bb0\u53bb\u9664\u7b56\u7565\uff0c\u5e76\u8ba8\u8bba\u86cb\u767d\u8d28\u7eaf\u5316\u7cfb\u7edf\u7684\u672a\u6765\u65b9\u5411\u3002<\/span><\/p>\n<p style=\"margin-bottom: 0in;\"><br  \/><\/p>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\"  >\n<section class=\"135brush\"   style=\"color: rgb(5, 115, 175);padding: 0.45em 0.6em 0.45em 0.9em;border-left: 10px solid rgb(83, 191, 250);border-top-color: rgb(83, 191, 250);border-right-color: rgb(83, 191, 250);border-bottom-color: rgb(83, 191, 250);background: rgb(182, 228, 253);box-sizing: border-box;\">1.\u4e3b\u8981\u7684\u6807\u7b7e\u79cd\u7c7b<\/section>\n<\/section>\n<\/section>\n<p style=\"text-align: center;margin-bottom: 0in;\"><img loading=\"lazy\" align=\"bottom\" border=\"0\" class=\"\"        height=\"440\" hspace=\"1\" style=\"width: 100%;height: auto;\" width=\"377\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/8-1558084240.jpg\" alt=\"\u4eb2\u548c\u6807\u7b7e\u5728\u91cd\u7ec4\u86cb\u767d\u8868\u8fbe\u4e0e\u7eaf\u5316\u7684\u4f7f\u7528\u53ca\u6bd4\u8f83\" title=\"\u4eb2\u548c\u6807\u7b7e\u5728\u91cd\u7ec4\u86cb\u767d\u8868\u8fbe\u4e0e\u7eaf\u5316\u7684\u4f7f\u7528\u53ca\u6bd4\u8f83\"  \/><\/p>\n<p style=\"text-align: center;margin-bottom: 0in;\"><span style=\"font-family: \u5b8b\u4f53, SimSun;font-size: 10px;\">\u4e3b\u8981\u878d\u5408\u6807\u7b7e\u7684\u4f18\u7f3a\u70b9\u6bd4\u8f83<\/span><\/p>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"margin-top: 1em;margin-bottom: 1em;font-size: 14px;line-height: 20px;text-align: left;clear: both;\">\n<section style=\"display: inline-block;padding: 5px 10px;color: rgb(254, 254, 254);font-weight: bold;border-top-left-radius: 3px;border-bottom-left-radius: 3px;background-color: rgb(128, 177, 53);box-sizing: border-box;\" class=\"autonum\"  title=\"\">1.1<\/section>\n<section style=\"display: inline-block;padding: 5px 10px;color: rgb(254, 254, 254);font-weight: bold;border-top-right-radius: 3px;border-bottom-right-radius: 3px;background-color: rgb(176, 214, 118);box-sizing: border-box;\" class=\"135brush\"   >\u7ec4\u6c28\u9178\u6807\u7b7e\u548c\u56fa\u76f8\u91d1\u5c5e\u4eb2\u548c\u8272\u8c31\uff08IMAC\uff09<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 0.3in;margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">His-tag\u662f\u76ee\u524d<strong>IMAC<\/strong>\u6700\u6d41\u884c\u7684\u9009\u62e9\uff0c\u5177\u6709\u5c3a\u5bf8\u5c0f\u3001\u6d17\u8131\u6761\u4ef6\u6e29\u548c\u4ee5\u53ca\u8868\u8fbe\u8f7d\u4f53\u7684\u5e7f\u6cdb\u9002\u7528\u6027\u7684\u4f18\u70b9\u3002\u6b64\u5916\uff0c\u9488\u5bf9His-tag\u7684\u6297\u4f53\u4e5f\u53ef\u5546\u4e1a\u83b7\u5f97\uff0c\u53ef\u7528\u4e8e\u8bc6\u522b\u8868\u8fbe\u86cb\u767d\u3002\u56e0\u6b64\u8bb8\u591a\u7ed3\u6676\u5b66\u548c\u6838\u78c1\u5171\u632f\u86cb\u767d\u7814\u7a76\u4e5f\u91c7\u7528His-tag\u3002\u5c3d\u7ba1His-tag\u5177\u6709\u8bb8\u591a\u4f18\u70b9\uff0c\u4f46\u7531\u4e8e\u7ec6\u83cc\u88c2\u89e3\u6db2\u4e2d\u901a\u5e38\u4e5f\u542b\u6709\u67d0\u4e9b\u91d1\u5c5e\u7ed3\u5408\u86cb\u767d\uff0c\u8fd9\u4e9b\u86cb\u767d\u4e5f\u4f1a\u7ed3\u5408\u5230\u5c42\u6790\u67f1\u4e0a\u3002\u56e0\u6b64His-tag\u7eaf\u5316\u7684\u7279\u5f02\u6027\u8f83\u5f31\uff0c\u5982\u679c\u8981\u5f97\u5230\u9ad8\u7eaf\u5ea6\u7684\u76ee\u7684\u86cb\u767d\uff0c\u8fd8\u9700\u8981\u589e\u52a0\u7eaf\u5316\u6b65\u9aa4\u3002\u6b64\u5916\uff0c\u5982\u679c\u6837\u54c1\u4e2d\u542b\u6709EDTA\u7b49\u7269\u8d28\uff0c\u4f1a\u4f7f\u586b\u6599\u4e0a\u7684\u91d1\u5c5e\u79bb\u5b50\u6389\u843d\uff0c\u6781\u5927\u7684\u5f71\u54cd\u7eaf\u5316\u3002<\/span><\/p>\n<p style=\"text-align: center;text-indent: 0.3in;margin-bottom: 0in;\"><img loading=\"lazy\" align=\"bottom\" border=\"0\" class=\"\"     height=\"215\" style=\"width: 215px;height: 215px;\" width=\"215\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/0-1558084241.jpg\" alt=\"\u4eb2\u548c\u6807\u7b7e\u5728\u91cd\u7ec4\u86cb\u767d\u8868\u8fbe\u4e0e\u7eaf\u5316\u7684\u4f7f\u7528\u53ca\u6bd4\u8f83\" title=\"\u4eb2\u548c\u6807\u7b7e\u5728\u91cd\u7ec4\u86cb\u767d\u8868\u8fbe\u4e0e\u7eaf\u5316\u7684\u4f7f\u7528\u53ca\u6bd4\u8f83\"  \/><\/p>\n<p style=\"text-align: center;text-indent: 0.3in;margin-bottom: 0in;\"><span style=\"font-family: \u5b8b\u4f53, SimSun;font-size: 10px;\">\u4e2d\u79d1\u68ee\u8f89\u5f00\u53d1\u7684\u7ec4\u6c28\u9178\u6807\u7b7e\u7eaf\u5316\u4ecb\u8d28<\/span><\/p>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"margin-top: 1em;margin-bottom: 1em;font-size: 14px;line-height: 20px;text-align: left;clear: both;\">\n<section style=\"display: inline-block;padding: 5px 10px;color: rgb(254, 254, 254);font-weight: bold;border-top-left-radius: 3px;border-bottom-left-radius: 3px;background-color: rgb(128, 177, 53);box-sizing: border-box;\" class=\"autonum\"  title=\"\" >1.2<\/section>\n<section style=\"display: inline-block;padding: 5px 10px;color: rgb(254, 254, 254);font-weight: bold;border-top-right-radius: 3px;border-bottom-right-radius: 3px;background-color: rgb(176, 214, 118);box-sizing: border-box;\" class=\"135brush\"   >\u8868\u4f4d\u6807\u7b7e\u7528\u4e8e\u514d\u75ab\u4eb2\u548c\u5206\u79bb<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 0.29in;margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">\u8868\u4f4d\u6807\u8bb0\u7531\u542b\u6709\u5df2\u77e5\u6297\u539f\u8868\u4f4d\u7684\u591a\u80bd\u5e8f\u5217\u7ec4\u6210\uff0c\u4e14\u6709\u5bf9\u5176\u6709\u9002\u5f53\u4eb2\u548c\u529b\u7684\u7279\u5f02\u6027\u6297\u4f53\u3002\u7531\u4e8e\u5229\u7528\u6742\u4ea4\u7624\u6280\u672f\u53ef\u83b7\u5f97\u5927\u91cf\u3001\u9ad8\u7eaf\u5ea6\u5355\u514b\u9686\u6297\u4f53\uff0c\u56e0\u6b64\u53ef\u5c06\u6297\u4f53\u56fa\u5b9a\u5230\u586b\u6599\u57fa\u8d28\u4e0a\uff0c\u5e76\u7528\u4e8e\u8868\u4f4d\u6807\u8bb0\u91cd\u7ec4\u86cb\u767d\u7684\u7eaf\u5316\u3002<\/span><\/p>\n<p style=\"margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\"><strong>1.2.1<\/strong><strong>FLAG<\/strong><\/span><\/p>\n<p style=\"text-indent: 0.29in;margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">FLAG\u6807\u7b7e\u7684\u5e8f\u5217\uff08DYKDDDDK\uff09\u7279\u610f\u8bbe\u8ba1\u4e3a\u5728\u9776\u86cb\u767d\u8868\u9762\u5177\u6709\u9ad8\u5ea6\u4eb2\u6c34\u6027\uff0c\u53ef\u4ee5\u51cf\u5c11\u5bf9\u86cb\u767d\u8d28\u6298\u53e0\u7684\u4e0d\u5229\u5f71\u54cd\u3002DDDDK\u7247\u6bb5\u5141\u8bb8\u7528\u80a0\u6fc0\u9176\u526a\u5207\u6807\u7b7e\u3002<\/span><\/p>\n<p style=\"margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\"><strong>1.2.2c-myc<\/strong><\/span><\/p>\n<p style=\"text-indent: 0.29in;margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">c-myc\u6807\u7b7e\uff08EQKLSEDEL\uff09\u5df2\u88ab\u7528\u4e8e\u591a\u79cd\u7528\u9014\uff0c\u5982\u6d41\u5f0f\u7ec6\u80de\u672f\u3001\u514d\u75ab\u6c89\u6dc0\u4ee5\u53ca\u86cb\u767d\u8d28\u7eaf\u5316\uff0c\u4f46\u7eaf\u5316\u5df2\u7ecf\u5f88\u5c11\u7528c-myc\u6807\u7b7e<\/span><\/p>\n<p style=\"margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\"><strong>1.2.3HA<\/strong><\/span><\/p>\n<p style=\"text-indent: 0.29in;margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">\u6d41\u611f\u75c5\u6bd2HA\u6807\u7b7e\u5305\u62ecYPYDVPDYA\u3002\u548cc-myc\u6807\u7b7e\u4e00\u6837\uff0cHA\u6807\u7b7e\u4e3b\u8981\u7528\u4e8e\u793a\u8e2a\uff0c\u68c0\u6d4b\u548c\u7eaf\u5316\u76ee\u7684\u86cb\u767d\u3002<\/span><\/p>\n<p style=\"margin-bottom: 0in;\"><br  \/><\/p>\n<p style=\"margin-bottom: 0in;text-indent: 2em;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">\u6297\u539f\u8868\u4f4d\u6807\u8bb0\u901a\u5e38\u88ab\u7528\u6765\u4ee3\u66ffHis-tag\u6807\u8bb0\uff0c\u56e0\u4e3a\u5b83\u4eec\u5177\u6709\u66f4\u9ad8\u7684\u7279\u5f02\u6027\u548c\u68c0\u6d4b\u80fd\u529b\u3002\u7136\u800c\u7531\u4e8e\u514d\u75ab\u4eb2\u548c\u6811\u8102\u6bd4\u4f20\u7edf\u4eb2\u548c\u6811\u8102\u66f4\u9ad8\u6602\u7684\u6210\u672c\uff0c\u4f7f\u7528\u6297\u4f53\u7279\u5f02\u6027\u8868\u4f4d\u6807\u8bb0\u8fdb\u884c\u7eaf\u5316\u66f4\u9002\u5408\u4e8e\u5c0f\u89c4\u6a21\u7eaf\u5316\u3002<\/span><\/p>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"margin-top: 1em;margin-bottom: 1em;font-size: 14px;line-height: 20px;text-align: left;clear: both;\">\n<section style=\"display: inline-block;padding: 5px 10px;color: rgb(254, 254, 254);font-weight: bold;border-top-left-radius: 3px;border-bottom-left-radius: 3px;background-color: rgb(128, 177, 53);box-sizing: border-box;\" class=\"autonum\"  title=\"\" >1.3<\/section>\n<section style=\"display: inline-block;padding: 5px 10px;color: rgb(254, 254, 254);font-weight: bold;border-top-right-radius: 3px;border-bottom-right-radius: 3px;background-color: rgb(176, 214, 118);box-sizing: border-box;\" class=\"135brush\"   >\u914d\u57fa\u7ed3\u5408\u4eb2\u548c\u6807\u7b7e<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 0.29in;margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">\u8fd9\u4e9b\u86cb\u767d\u6807\u7b7e\u901a\u5e38\u6bd4\u8868\u4f4d\u6807\u7b7e\u5927\uff0c\u5305\u542b\u80fd\u7ed3\u5408\u81f3\u56fa\u5b9a\u5728\u4eb2\u548c\u6811\u8102\u4e0a\u7684\u7279\u5b9a\u914d\u4f53\u7684\u529f\u80fd\u57df\/\u5e8f\u5217\u3002<\/span><\/p>\n<p style=\"margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\"><strong>1.3.1<\/strong><strong>\u9499\u8c03\u86cb\u767d\u7ed3\u5408\u80bd\uff08<\/strong><strong>CBP<\/strong><strong>\uff09<\/strong><\/span><\/p>\n<p style=\"text-indent: 0.29in;margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">CBP\u662f\u6765\u81ea\u9aa8\u9abc\u808c\u808c\u7403\u86cb\u767d\u8f7b\u94fe\u6fc0\u9176\u768426\u4e2a\u6c28\u57fa\u9178\u5e8f\u5217\uff0c\u5728\u9499\u79bb\u5b50\u5b58\u5728\u4e0b\u80fd\u4e0e\u9499\u8c03\u86cb\u767d\u7ed3\u5408\u3002\u4ece\u9499\u8c03\u7d20\u4eb2\u548c\u67f1\u4e2d\u6d17\u8131CBP\u878d\u5408\u86cb\u767d\u53ef\u4ee5\u5728\u751f\u7406\u6761\u4ef6\u4e0b\u8fdb\u884c\uff0c\u8fd9\u4f7f\u5f97\u8be5\u6807\u7b7e\u6210\u4e3a\u96be\u4ee5\u7eaf\u5316\u7684\u86cb\u767d\u8d28\uff08\u5982\u819c\u86cb\u767d\uff09\u7684\u4e00\u4e2a\u5438\u5f15\u4eba\u7684\u9009\u62e9\u3002\u7f3a\u70b9\u662f\u5176\u4ed6\u4e00\u4e9b\u771f\u6838\u86cb\u767d\u53ef\u80fd\u4e0e\u9499\u8c03\u86cb\u767d\u4eb2\u548c\u6811\u8102\u76f8\u4e92\u4f5c\u7528\u3002\u56e0\u6b64\uff0cCBP\u6807\u7b7e\u5e94\u8be5\u4e0e\u7ec6\u83cc\u8868\u8fbe\u7cfb\u7edf\u4e00\u8d77\u4f7f\u7528\u3002<\/span><\/p>\n<p style=\"margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\"><strong>1.3.2<\/strong><strong>\u8c37\u80f1\u7518\u80bd<\/strong><strong>S-<\/strong><strong>\u8f6c\u79fb\u9176\uff08<\/strong><strong>GST<\/strong><strong>\uff09<\/strong><\/span><\/p>\n<p style=\"text-indent: 0.29in;margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">GST\u6807\u7b7e\u662f\u4e00\u79cd\u5728\u771f\u6838\u751f\u7269\u4e2d\u53d1\u73b0\u768426kDa\u9176\uff0c\u80fd\u7ed3\u5408\u5230\u914d\u4f53\u8c37\u80f1\u7518\u80bd\u4e0a\u3002GST\u6807\u7b7e\u6700\u521d\u7528\u4e8epGEX\u8d28\u7c92\uff0c\u4ee5\u4fc3\u8fdb\u96be\u4ee5\u7eaf\u5316\u7684\u771f\u6838\u86cb\u767d\u7684\u7eaf\u5316\u3002\u5728\u878d\u5408\u5230\u76ee\u6807\u86cb\u767d\u7684N\u7aef\u65f6\uff0cGST\u6807\u7b7e\u53ef\u80fd\u80fd\u63d0\u9ad8\u76ee\u7684\u86cb\u767d\u7684\u53ef\u6eb6\u6027\u53ca\u8868\u8fbe\u7a0b\u5ea6\u3002GST\u53ef\u80fd\u4e3b\u8981\u901a\u8fc7\u5728\u805a\u96c6\u548c\u4e0d\u9002\u5f53\u6298\u53e0\u53d1\u751f\u524d\u7a33\u5b9a\u9776\u86cb\u767d\u7684\u6298\u53e0\u6765\u6539\u5584\u86cb\u767d\u8d28\u7684\u8868\u8fbe\uff0c\u800c\u4e0d\u662f\u901a\u8fc7\u63d0\u9ad8\u6eb6\u89e3\u6027\u6765\u6539\u5584\u8868\u8fbe\u3002<\/span><\/p>\n<p style=\"text-align: center;text-indent: 0.29in;margin-bottom: 0in;\"><img loading=\"lazy\" align=\"bottom\" border=\"0\" class=\"\"     height=\"223\" hspace=\"1\" style=\"width: 223px;height: 223px;\" vspace=\"1\" width=\"223\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/7-1558084241.jpg\" alt=\"\u4eb2\u548c\u6807\u7b7e\u5728\u91cd\u7ec4\u86cb\u767d\u8868\u8fbe\u4e0e\u7eaf\u5316\u7684\u4f7f\u7528\u53ca\u6bd4\u8f83\" title=\"\u4eb2\u548c\u6807\u7b7e\u5728\u91cd\u7ec4\u86cb\u767d\u8868\u8fbe\u4e0e\u7eaf\u5316\u7684\u4f7f\u7528\u53ca\u6bd4\u8f83\"  \/><\/p>\n<p style=\"text-align: center;text-indent: 0.3in;margin-bottom: 0in;\"><span style=\"font-family: \u5b8b\u4f53, SimSun;font-size: 10px;\">\u4e2d\u79d1\u68ee\u8f89\u5f00\u53d1\u7684GST\u6807\u7b7e\u7eaf\u5316\u4ecb\u8d28<\/span><\/p>\n<p style=\"text-align:center;text-indent: 0.29in;margin-bottom: 0in;\"><br  \/><\/p>\n<p style=\"margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\"><strong>1.3.3<\/strong><strong>\u94fe\u9709\u4eb2\u548c\u7d20\u7ed3\u5408\u80bd<\/strong><\/span><\/p>\n<p style=\"text-indent: 0.29in;margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">\u94fe\u9709\u4eb2\u548c\u7d20\u7ed3\u5408\u6807\u7b7e\u4ec5\u7531\u5c11\u6570\u6c28\u57fa\u9178\u7ec4\u6210\uff0c\u5e76\u80fd\u7ed3\u5408\u5230\u4e00\u79cd\u7ec6\u83cc\u86cb\u767d\u8d28\u94fe\u9709\u4eb2\u548c\u7d20\u4e0a\u3002\u591a\u5e74\u6765\uff0cStreptavidin\u6807\u7b7e\u5e8f\u5217\u4eceAWRHPQFGG\u6539\u4e3aNWSHPQFEK\u3002\u8fd9\u79cd\u65b0\u7684strep-II\u6807\u7b7e\u5141\u8bb8\u5c06\u6807\u7b7e\u653e\u7f6e\u5728N-\u672b\u7aef\uff0c\u800c\u4e0d\u50cf\u6700\u521d\u53ea\u80fd\u5728\u76ee\u6807\u86cb\u767d\u7684C-\u672b\u7aef\u3002\u4e0eHis-tag\u76f8\u6bd4\uff0cStrepII\u7684\u6811\u8102\u66f4\u8d35\uff0c\u4f46\u7279\u5f02\u6027\u66f4\u5f3a\uff0c\u7eaf\u5316\u7eaf\u5ea6\u66f4\u9ad8\uff0c\u751a\u81f3\u53ef\u4ee5\u4e0e\u5404\u79cd\u514d\u75ab\u6811\u8102\u76f8\u5ab2\u7f8e\uff0c\u4f46\u76f8\u6bd4\u4e4b\u4e0b\u6210\u672c\u663e\u8457\u66f4\u4f4e\u3002\u56e0\u6b64\uff0cStrepII\u6807\u7b7e\u53ef\u80fd\u662f\u914d\u4f53\u6807\u7b7e\u4e2d\u6700\u9002\u5408\u5927\u89c4\u6a21\u7eaf\u5316\u7684\u3002<\/span><\/p>\n<p style=\"margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\"><strong>1.3.4<\/strong><strong>\u9ea6\u82bd\u7cd6\u7ed3\u5408\u86cb\u767d<\/strong><\/span><\/p>\n<p style=\"text-indent: 0.29in;margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">MBP\u6807\u7b7e\u662f\u4e00\u79cd\u6765\u6e90\u4e8e\u5927\u80a0\u6746\u83cc\u768442kDa\u7684\u86cb\u767d\uff0c\u7531\u4e8e\u5176\u6eb6\u89e3\u6027\u589e\u5f3a\u4f5c\u7528\u800c\u88ab\u5e7f\u6cdb\u7528\u4f5c\u8f7d\u4f53\u86cb\u767d\u3002MBP\u5bf9\u4ea4\u8054\u7684\u6dc0\u7c89\u548c\u9ea6\u82bd\u7cd6\u5177\u6709\u5f88\u9ad8\u7684\u4eb2\u548c\u529b\u3002\u7136\u800c\uff0cMBP\u878d\u5408\u5230\u91cd\u7ec4\u86cb\u767d\u7684C\u7aef\u53ef\u80fd\u5f71\u54cd\u878d\u5408\u86cb\u767d\u5bf9\u6dc0\u7c89\/\u9ea6\u82bd\u7cd6\u7684\u4eb2\u548c\u529b\uff0c\u56e0\u4e3a\u878d\u5408\u86cb\u767d\u53ef\u80fd\u4f1a\u548c\u5176\u7ed3\u5408\u4f4d\u70b9\u76f8\u4e92\u4f5c\u7528\u3002\u56e0\u6b64\uff0c\u901a\u5e38\u9700\u8981\u5728MBP\u7684N-\u7aef\u653e\u7f6eHis-tag\uff0c\u4ee5\u4fc3\u8fdb\u878d\u5408\u86cb\u767d\u7684\u7eaf\u5316\u3002MBP\u5728\u63d0\u9ad8\u878d\u5408\u86cb\u767d\u7684\u6eb6\u89e3\u6027\u65b9\u9762\u4e5f\u975e\u5e38\u6709\u6548\u3002<\/span><\/p>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"margin-top: 1em;margin-bottom: 1em;font-size: 14px;line-height: 20px;text-align: left;clear: both;\">\n<section style=\"display: inline-block;padding: 5px 10px;color: rgb(254, 254, 254);font-weight: bold;border-top-left-radius: 3px;border-bottom-left-radius: 3px;background-color: rgb(128, 177, 53);box-sizing: border-box;\" class=\"autonum\"  title=\"\" >1.4<\/section>\n<section style=\"display: inline-block;padding: 5px 10px;color: rgb(254, 254, 254);font-weight: bold;border-top-right-radius: 3px;border-bottom-right-radius: 3px;background-color: rgb(176, 214, 118);box-sizing: border-box;\" class=\"135brush\"   >\u5176\u5b83\u878d\u5408\u8868\u8fbe\u4f34\u4fa3<\/section>\n<\/section>\n<\/section>\n<p style=\"margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\"><strong>1.4.1<\/strong><strong>\u5f39\u6027\u86cb\u767d\u6837\u591a\u80bd<\/strong><\/span><\/p>\n<p style=\"text-indent: 0.29in;margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">ELP\u753120-330\u4e2a\u4e94\u80bdVal-Pro-Gly-Xaa-Gly\u91cd\u590d\u7ec4\u6210\uff0c\u5e76\u901a\u8fc7\u6807\u7b7e\u7684\u6e29\u5ea6\u4f9d\u8d56\u6027\u4fc3\u8fdb\u76ee\u7684\u86cb\u767d\u7684\u7eaf\u5316\u3002\u6839\u636e\u4e94\u80bd\u91cd\u590d\u5e8f\u5217\u7684\u6570\u76ee\u548c\u5e8f\u5217\uff0cELP\u878d\u5408\u86cb\u767d\u5728\u7279\u5b9a\u6e29\u5ea6\u4e0b\u53ef\u7ecf\u5386\u53ef\u9006\u7684\u76f8\u53d8\uff0c\u5e76\u4ece\u6eb6\u6db2\u4e2d\u6c89\u6dc0\u51fa\u6765\u3002\u8fd9\u6837\u53ef\u4ee5\u53bb\u9664\u4e0a\u6e05\u6db2\u4e2d\u7684\u5176\u4ed6\u86cb\u767d\u8d28\uff0c\u540c\u65f6\u5206\u79bb\u6c89\u6dc0\u7684ELP\u878d\u5408\u86cb\u767d\u3002ELP\u6807\u7b7e\u7684\u4e00\u4e2a\u4f18\u70b9\u662f\u5b83\u4e0d\u9700\u8981\u7279\u6b8a\u7684\u6811\u8102\u6216\u67f1\uff0c\u53ea\u9700\u79bb\u5fc3\u5c31\u53ef\u4ee5\u3002\u7136\u800c\uff0c\u7531\u4e8e\u8fd9\u4e00\u7b56\u7565\u5229\u7528\u4e86\u878d\u5408\u86cb\u767d\u7684\u6eb6\u89e3\u6027\u53d8\u5316\uff0c\u56e0\u6b64\u9700\u8981\u8003\u5bdfELP\u7684\u7c7b\u578b\u4ee5\u53ca\u878d\u5408\u7684\u4f4d\u70b9\uff0c\u4ee5\u4ea7\u751f\u80fd\u9002\u5f53\u6298\u53e0\u548c\u4e14\u5177\u6709\u751f\u7406\u6d3b\u6027\u7684\u91cd\u7ec4\u86cb\u767d\u3002<\/span><\/p>\n<p style=\"margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\"><strong>1.4.2<\/strong><strong>\u786b\u6c27\u8fd8\u86cb\u767d<\/strong><strong>A<\/strong><strong>\uff08<\/strong><strong>Trx<\/strong><strong>\uff09<\/strong><\/span><\/p>\n<p style=\"text-indent: 0.29in;margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">\u786b\u6c27\u8fd8\u86cb\u767dA\u662f\u4e00\u79cd12kDa\u7684\u9176\uff0c\u50ac\u5316\u5404\u79cd\u8fd8\u539f\u6c27\u5316\u53cd\u5e94\u3002Trx\u80fd\u663e\u8457\u964d\u4f4e\u76ee\u7684\u86cb\u767d\u5f62\u6210\u5305\u6db5\u4f53\u7684\u51e0\u7387\u3002\u91ce\u751f\u578bTrx\u672c\u8eab\u5e76\u4e0d\u80fd\u901a\u8fc7\u4eb2\u548c\u8272\u8c31\u6cd5\u8fdb\u884c\u878d\u5408\u86cb\u767d\u7684\u7eaf\u5316\u3002\u4e3a\u4e86\u89e3\u51b3\u8fd9\u4e00\u95ee\u9898\uff0c\u7a81\u53d8\u7684\u786b\u6c27\u8fd8\u86cb\u767d\u88ab\u8bbe\u8ba1\u6210\u5177\u6709\u542b\u6709\u7ec4\u6c28\u9178\u6807\u7b7e\uff0c\u6216\u5728\u4f53\u5185\u6613\u751f\u7269\u7d20\u5316\uff0c\u7136\u540e\u901a\u8fc7\u4eb2\u548c\u67f1\u6355\u83b7\u3002<\/span><\/p>\n<p style=\"margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\"><strong>1.4.3<\/strong><strong>NusA<\/strong><\/span><\/p>\n<p style=\"text-indent: 0.28in;margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">NusA\u662f\u4e00\u79cd55kDa\u7684\u5927\u80a0\u6746\u83cc\u86cb\u767d\uff0c\u5176\u5728\u8fc7\u5ea6\u8868\u8fbe\u65f6\u5177\u6709\u5f88\u9ad8\u7684\u6eb6\u89e3\u6027\uff0c\u5728\u91cd\u7ec4\u86cb\u767d\u8868\u8fbe\u548c\u7eaf\u5316\u8fc7\u7a0b\u4e2d\u80fd\u589e\u52a0\u5176\u878d\u5408\u86cb\u767d\u7684\u6eb6\u89e3\u6027\u3002\u4e0e\u9ea6\u82bd\u7cd6\u7ed3\u5408\u86cb\u767d\u76f8\u6bd4\uff0c\u5c3d\u7ba1NusA\u5177\u6709\u975e\u5e38\u4e0d\u540c\u7684\u7ed3\u6784\u548c\u5316\u5b66\u6027\u8d28\uff0c\u4f46\u5176\u6eb6\u89e3\u6027\u589e\u5f3a\u6548\u679c\u5927\u81f4\u76f8\u540c\u3002\u7531\u4e8eNusA\u4e0e\u7279\u5b9a\u7684\u6811\u8102\u6ca1\u6709\u5f88\u5f3a\u7684\u7ed3\u5408\u4eb2\u548c\u529b\uff0c\u5b83\u88ab\u8bbe\u8ba1\u6210\u5177His-tag\u7684\u8868\u8fbe\u8f7d\u4f53\u4ee5\u65b9\u4fbf\u7eaf\u5316\u3002<\/span><\/p>\n<p style=\"margin-bottom: 0in;\"><br  \/><\/p>\n<p style=\"margin-bottom: 0in;text-indent: 2em;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">\u4ee5\u4e0a\u6211\u4eec\u53ef\u4ee5\u603b\u7ed3\u51fa\uff0c\u5e7f\u6cdb\u4f7f\u7528\u7684\u86cb\u767d\u8d28\u7ed3\u6784\u57df\u6807\u7b7e\u5177\u6709\u5171\u540c\u7684\u7279\u6027\uff1a\u589e\u52a0\u4e86\u91cd\u7ec4\u86cb\u767d\u7684\u6eb6\u89e3\u6027\u548c\u8868\u8fbe\u6c34\u5e73\u3002\u8fd9\u4e24\u79cd\u589e\u5f3a\u6548\u5e94\u662f\u901a\u8fc7\u4e00\u79cd\u76ee\u524d\u5c1a\u4e0d\u6e05\u695a\u7684\u673a\u5236\u4ea7\u751f\u7684\uff0c\u56e0\u4e3a\u5373\u4f7f\u662f\u5316\u5b66\u4e0a\u4e0d\u540c\u7684\u7ed3\u6784\uff0c\u5982MBP\u548c<strong>NusA<\/strong>\uff0c\u4e5f\u53ef\u80fd\u5177\u6709\u7c7b\u4f3c\u7684\u86cb\u767d\u8d28\u8868\u8fbe\u548c\u6eb6\u89e3\u4f5c\u7528\u3002\u6709\u5047\u8bbe\u8ba4\u4e3a\u8fd9\u4e9b\u8f7d\u4f53\u86cb\u767d\u53ef\u80fd\u4f5c\u4e3a\u4f34\u4fa3\u4fc3\u8fdb\u6765\u76ee\u7684\u86cb\u767d\u8d28\u7684\u6298\u53e0\u3002<\/span><\/p>\n<p style=\"margin-bottom: 0in;\"><br  \/><\/p>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\"  >\n<section class=\"135brush\"   style=\"color: rgb(5, 115, 175);padding: 0.45em 0.6em 0.45em 0.9em;border-left: 10px solid rgb(83, 191, 250);border-top-color: rgb(83, 191, 250);border-right-color: rgb(83, 191, 250);border-bottom-color: rgb(83, 191, 250);background: rgb(182, 228, 253);box-sizing: border-box;\">2.\u878d\u5408\u6807\u7b7e\u7684\u53bb\u9664<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 0.29in;margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">\u5728\u901a\u8fc7\u4eb2\u548c\u529b\u5206\u79bb\u7eaf\u5316\u76ee\u7684\u86cb\u767d\u540e\uff0c\u5982\u679c\u6807\u7b7e\u7684\u5b58\u5728\u5c06\u663e\u8457\u5f71\u54cd\u5176\u751f\u7269\u529f\u80fd\u6216\u9002\u7528\u8be5\u86cb\u767d\u7684\u5b89\u5168\u6027\uff0c\u5219\u9700\u8981\u80fd\u591f\u79fb\u9664\u8fd9\u4e9b\u6807\u7b7e\uff0c\u5e76\u4e14\u8fd9\u4e00\u64cd\u4f5c\u901a\u5e38\u65f6\u6709\u5fc5\u8981\u7684\u3002\u8bb8\u591a\u7b56\u7565\u88ab\u8bbe\u8ba1\u7528\u6765\u5728\u7eaf\u5316\u540e\u53bb\u9664\u6807\u7b7e\u3002<\/span><\/p>\n<p style=\"margin-bottom: 0in;text-align: left;text-indent: 0em;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\"><img loading=\"lazy\" align=\"bottom\" border=\"0\" class=\"\"        height=\"374\" vspace=\"1\" width=\"370\" style=\"height: 556px;font-size: 16px;text-indent: 27.84px;white-space: normal;width: 553px;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/7-1558084241-1.jpg\" alt=\"\u4eb2\u548c\u6807\u7b7e\u5728\u91cd\u7ec4\u86cb\u767d\u8868\u8fbe\u4e0e\u7eaf\u5316\u7684\u4f7f\u7528\u53ca\u6bd4\u8f83\" title=\"\u4eb2\u548c\u6807\u7b7e\u5728\u91cd\u7ec4\u86cb\u767d\u8868\u8fbe\u4e0e\u7eaf\u5316\u7684\u4f7f\u7528\u53ca\u6bd4\u8f83\"  \/><\/span><\/p>\n<p style=\"text-indent: 0.29in;margin-bottom: 0in;text-align: center;\"><span style=\"font-size: 12px;\">\u5e38\u7528\u7684\u6807\u7b7e\u53bb\u9664\u65b9\u6cd5\u53ca\u5176\u4f18\u7f3a\u70b9<\/span><\/p>\n<section class=\"autonum\"  title=\"\"  style=\"font-size: 14px;text-align: left;display: inline-block;padding: 5px 10px;color: rgb(254, 254, 254);font-weight: bold;border-top-left-radius: 3px;border-bottom-left-radius: 3px;background-color: rgb(128, 177, 53);box-sizing: border-box;\">2.1<\/section>\n<section class=\"135brush\"    style=\"font-size: 14px;text-align: left;display: inline-block;padding: 5px 10px;color: rgb(254, 254, 254);font-weight: bold;border-top-right-radius: 3px;border-bottom-right-radius: 3px;background-color: rgb(176, 214, 118);box-sizing: border-box;\">\u51dd\u8840\u9176<\/section>\n<p style=\"text-indent: 0.29in;margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">\u8fd9\u79cd37kDa\u7684\u4e1d\u6c28\u9178\u86cb\u767d\u9176\u88ab\u5e7f\u6cdb\u4f7f\u7528\uff0c\u901a\u8fc7\u5207\u5272LVPR*GS\u7684\u51dd\u8840\u9176\u8bc6\u522b\u5e8f\u5217\uff0c\u4ece\u9776\u86cb\u767d\u4e2d\u53bb\u9664His-tag\u548c\u5176\u4ed6\u6807\u7b7e\u3002\u5c3d\u7ba1\u51dd\u8840\u9176\u8bc6\u522b\u7684\u5e8f\u5217\u662f\u76f8\u5bf9\u7279\u5f02\u7684\uff0c\u4f46\u6709\u62a5\u9053\u79f0\uff0c\u51dd\u8840\u9176\u7684\u5546\u4e1a\u5236\u5907\u4f1a\u5bfc\u81f4\u975e\u7279\u5f02\u6027\u9176\u5207\u3002\u51dd\u8840\u9176\u6bd4\u5176\u4ed6\u86cb\u767d\u9176\u7684\u4e00\u4e2a\u4f18\u70b9\u662f\uff0c\u5b83\u80fd\u591f\u5728\u591a\u79cd\u8868\u9762\u6d3b\u6027\u5242\u5b58\u5728\u4e0b\u4fdd\u6301\u9ad8\u6d3b\u6027\u3002\u56e0\u6b64\uff0c\u51dd\u8840\u9176\u53ef\u7528\u4e8e\u7eaf\u5316\u819c\u86cb\u767d\u3002<\/span><\/p>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"margin-top: 1em;margin-bottom: 1em;font-size: 14px;line-height: 20px;text-align: left;clear: both;\">\n<section style=\"display: inline-block;padding: 5px 10px;color: rgb(254, 254, 254);font-weight: bold;border-top-left-radius: 3px;border-bottom-left-radius: 3px;background-color: rgb(128, 177, 53);box-sizing: border-box;\" class=\"autonum\"  title=\"\" >2.2<\/section>\n<section style=\"display: inline-block;padding: 5px 10px;color: rgb(254, 254, 254);font-weight: bold;border-top-right-radius: 3px;border-bottom-right-radius: 3px;background-color: rgb(176, 214, 118);box-sizing: border-box;\" class=\"135brush\"   >TEV\u86cb\u767d\u9176<\/section>\n<\/section>\n<\/section>\n<p style=\"margin-bottom: 0in;text-indent: 2em;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">TEV\u86cb\u767d\u9176\u662f\u5728\u70df\u8349\u8680\u523b\u75c5\u6bd2\u4e2d\u53d1\u73b0\u7684\u4e00\u79cd\u9176\u768427kDa\u7684C\u7aef\u50ac\u5316\u4e9a\u5355\u4f4d\u3002\u5b83\u5bf9\u5e8f\u5217ENLYFQ*S.\u5177\u6709\u9ad8\u5ea6\u7279\u5f02\u6027\u3002\u6700\u521d\u53d1\u73b0\u7ecf\u8fc7\u957f\u65f6\u95f4\u7684\u57f9\u517b\uff0c\u8be5\u86cb\u767d\u9176\u4f1a\u81ea\u6211\u6d88\u5316\u3002\u7136\u800c\uff0c\u901a\u8fc7\u5bf9\u5176\u8fdb\u884c\u5404\u79cd\u7a81\u53d8\u4fee\u6539\uff0c\u5df2\u53ef\u4ee5\u4f5c\u4e3a\u53bb\u9664\u6807\u7b7e\u7684\u5de5\u5177\u3002\u7814\u7a76\u53d1\u73b0\uff0cS219V\u7a81\u53d8\u4f7f\u5176\u86cb\u767d\u6c34\u89e3\u9176\u6d3b\u6027\u589e\u52a0\u4e86\u81f3\u5c11\u4e24\u500d\uff0c\u540c\u65f6\u4e5f\u964d\u4f4e\u4e86\u81ea\u8eab\u964d\u89e3\u7684\u654f\u611f\u6027\u3002\u6b64\u5916\uff0c\u7f3a\u4e4fC\u7aef238-242\u6b8b\u57fa\u7684\u86cb\u767d\u9176\u53d8\u4f53\u5177\u6709\u66f4\u9ad8\u7684\u8868\u8fbe\u91cf\u548c\u50ac\u5316\u6d3b\u6027\u3002TEV\u86cb\u767d\u9176\u7684\u4e3b\u8981\u7f3a\u70b9\u662f\u4e0e\u5176\u4ed6\u86cb\u767d\u9176\u76f8\u6bd4\u8f6c\u5316\u7387\u8f83\u4f4e\u3002\u5176\u8f83\u4f4e\u7684\u8f6c\u5316\u7387\u53ef\u80fd\u89e3\u91ca\u4e86\u5176\u5bf9\u8bc6\u522b\u5e8f\u5217\u7684\u9ad8\u5ea6\u7279\u5f02\u6027\uff0c\u4f46\u5bfc\u81f4\u6d88\u5316\u65f6\u95f4\u957f\u548c\u6d88\u5316\u6240\u9700\u7684\u86cb\u767d\u9176\u91cf\u8f83\u9ad8\u3002\u7136\u800c\u4e00\u4e2a\u4f18\u52bf\u662f\uff0c\u8bc6\u522b\u5e8f\u5217\u4e2d\u7684\u4e1d\u6c28\u9178\u53ef\u4ee5\u8f6c\u53d8\u6210\u86cb\u6c28\u9178\uff0c\u5728\u9176\u5207\u540e\u80fd\u4ea7\u751f\u4e00\u4e2a\u201c\u5929\u7136\u201d\u7684N\u672b\u7aef\u3002\u7531\u4e8eTEV\u86cb\u767d\u9176\u5207\u4f4d\u70b9\u7684\u5e7f\u6cdb\u6027\u548c\u4f4e\u6210\u672c\uff0c\u4e5f\u662f\u5927\u89c4\u6a21\u7eaf\u5316\u4e2d\u5f88\u597d\u7684\u9009\u62e9\u3002<\/span><\/p>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"margin-top: 1em;margin-bottom: 1em;font-size: 14px;line-height: 20px;text-align: left;clear: both;\">\n<section style=\"display: inline-block;padding: 5px 10px;color: rgb(254, 254, 254);font-weight: bold;border-top-left-radius: 3px;border-bottom-left-radius: 3px;background-color: rgb(128, 177, 53);box-sizing: border-box;\" class=\"autonum\"  title=\"\" >2.3<\/section>\n<section style=\"display: inline-block;padding: 5px 10px;color: rgb(254, 254, 254);font-weight: bold;border-top-right-radius: 3px;border-bottom-right-radius: 3px;background-color: rgb(176, 214, 118);box-sizing: border-box;\" class=\"135brush\"   >3C\u86cb\u767d\u9176<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 0.29in;margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">3C\u86cb\u767d\u9176\u662f\u4e00\u79cd\u6765\u6e90\u4e8e\u4eba\u9f3b\u75c5\u6bd2\u768448kDa\u5927\u5c0f\u7684\u91cd\u7ec4\u534a\u80f1\u6c28\u9178\u86cb\u767d\u9176\u3002\u4e0eTEV\u86cb\u767d\u9176\u4e00\u6837\uff0c\u5b83\u5bf9\u5176\u8bc6\u522b\u5e8f\u5217LEVLFQ*GP\u5177\u6709\u9ad8\u5ea6\u7279\u5f02\u6027\u30023C\u86cb\u767d\u9176\u4e5f\u88ab\u8bbe\u8ba1\u6210\u542b\u6709\u4e00\u4e2a\u4eb2\u548c\u6807\u7b7e\uff0c\u5b83\u6709\u52a9\u4e8e\u5728\u88c2\u89e3\u540e\u5c06\u5176\u4ece\u7eaf\u5316\u86cb\u767d\u6eb6\u6db2\u4e2d\u53bb\u9664\u3002\u4e3b\u8981\u7f3a\u70b9\u662f\u9176\u5207\u540e\u4e0d\u80fd\u76ee\u7684\u86cb\u767d\u4e0d\u80fd\u5f62\u6210\u5929\u7136N\u672b\u7aef\u3002\u53e6\u4e00\u65b9\u9762\uff0c\u4e0eTEV\u86cb\u767d\u9176\u76f8\u6bd4\uff0c3C\u86cb\u767d\u9176\u57284\u6444\u6c0f\u5ea6\u4e0b\u670910\u500d\u4ee5\u4e0a\u7684\u6d3b\u6027\u3002<\/span><\/p>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"margin-top: 1em;margin-bottom: 1em;font-size: 14px;line-height: 20px;text-align: left;clear: both;\">\n<section style=\"display: inline-block;padding: 5px 10px;color: rgb(254, 254, 254);font-weight: bold;border-top-left-radius: 3px;border-bottom-left-radius: 3px;background-color: rgb(128, 177, 53);box-sizing: border-box;\" class=\"autonum\"  title=\"\" >2.4<\/section>\n<section style=\"display: inline-block;padding: 5px 10px;color: rgb(254, 254, 254);font-weight: bold;border-top-right-radius: 3px;border-bottom-right-radius: 3px;background-color: rgb(176, 214, 118);box-sizing: border-box;\" class=\"135brush\"   >\u5185\u86cb\u767d\u4ecb\u5bfc\u7684\u81ea\u526a\u5207<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 0.29in;margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">\u5185\u86cb\u767d\u5728\u7ec6\u83cc\u548c\u9175\u6bcd\u83cc\u4e2d\u53d1\u73b0\u7684\u80fd\u591f\u81ea\u6211\u5207\u9664\u7684\u86cb\u767d\u8d28\uff0c\u5e76\u4e14\u5df2\u7ecf\u88ab\u6539\u8fdb\u6210\u4e3a\u80fd\u591f\u5728N\u672b\u7aef\u6216C\u672b\u7aef\u8fdb\u884c\u526a\u5207\u4ee5\u8fbe\u5230\u76ee\u7684\u86cb\u767d\u6807\u7b7e\u53bb\u9664\u7684\u76ee\u7684\u3002\u4f8b\u5982\u6765\u81ea\u7ed3\u6838\u5206\u679d\u6746\u83cc\u7684\u5fae\u5c0f\u5185\u80bd\u86cb\u767dI-CM\u7a81\u53d8\u4f53\u5df2\u88ab\u8bbe\u8ba1\u6210\u6709\u5229\u4e8eC\u672b\u7aef\u526a\u5207\u3002\u5f53pH\u503c\u53d8\u62106\u65f6\uff0cC\u7aef\u5230I-CM\u86cb\u767d\u7684\u5e8f\u5217\u5c06\u88ab\u526a\u5207\u3002\u5982\u679c\u5c06\u4e00\u4e2a\u4eb2\u548c\u6807\u7b7e\u878d\u5408\u5728\u5185\u86cb\u767d\u7684N\u7aef\uff0c\u76ee\u7684\u86cb\u767d\u5728C\u7aef\uff0c\u5219\u53ef\u5728\u4e00\u5b9a\u6761\u4ef6\u4e0b\u5b9e\u73b0\u65e0\u6807\u7b7e\u86cb\u767d\u7684\u7eaf\u5316\u3002\u8be5\u7cfb\u7edf\u7684\u4f18\u70b9\u662f\u4e0d\u9700\u8981\u4f7f\u7528\u86cb\u767d\u9176\u6765\u5207\u5272\u4eb2\u548c\u6807\u7b7e\u3002\u7f3a\u70b9\u662f\u5fc5\u987b\u5c06\u86cb\u767d\u5206\u6210\u4e24\u90e8\u5206\u8fdb\u884c\u7eaf\u5316\uff0c\u4ee5\u4fbf\u4e8e\u7eaf\u5316\u540e\u7684\u53cd\u5f0f\u526a\u5207\uff0c\u5e76\u4e14\u53ef\u80fd\u7559\u6709\u989d\u5916\u7684\u6c28\u57fa\u9178\u6b8b\u57fa\u3002\u6b64\u5916\u5728\u5ba4\u6e29\u4e0b\u5b8c\u5168\u526a\u5207\u9700\u8981\u6bd4\u86cb\u767d\u9176\u526a\u5207\u66f4\u957f\u7684\u65f6\u95f4\u3002\u7531\u4e8e\u7528\u4e8e\u86cb\u767d\u8d28\u7eaf\u5316\u7684\u5185\u86cb\u767d\u79cd\u7c7b\u7e41\u591a\uff0c\u76ee\u524d\u8fd8\u6ca1\u6709\u4e00\u4e2a\u53ef\u4ee5\u5e94\u7528\u4e8e\u5404\u79cd\u4e0d\u540c\u6027\u8d28\u7684\u86cb\u767d\u8d28\u7684\u6807\u51c6\u6216\u901a\u7528\u65b9\u6848\u3002<\/span><\/p>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"margin-top: 1em;margin-bottom: 1em;font-size: 14px;line-height: 20px;text-align: left;clear: both;\">\n<section style=\"display: inline-block;padding: 5px 10px;color: rgb(254, 254, 254);font-weight: bold;border-top-left-radius: 3px;border-bottom-left-radius: 3px;background-color: rgb(128, 177, 53);box-sizing: border-box;\" class=\"autonum\"  title=\"\" >2.5<\/section>\n<section style=\"display: inline-block;padding: 5px 10px;color: rgb(254, 254, 254);font-weight: bold;border-top-right-radius: 3px;border-bottom-right-radius: 3px;background-color: rgb(176, 214, 118);box-sizing: border-box;\" class=\"135brush\"   >\u5c0f\u6cdb\u7d20\u76f8\u5173\u4fee\u9970\u5242\uff08SUMO\uff09<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 0.29in;margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">\u7528\u4e8e\u86cb\u767d\u8d28\u7eaf\u5316\u7684\u6700\u5e38\u89c1\u7684<strong>SUMO<\/strong>\u86cb\u767d\u9176\u662fUlp1\uff0c\u4e00\u79cd27kDa\u7684\u9175\u6bcd\u9176\uff0c\u80fd\u591f\u5c06<strong>SUMO<\/strong>\u90e8\u5206\u4ece\u9776\u86cb\u767d\u4e2d\u53bb\u9664\u3002\u4e0e\u5176\u4ed6\u8bc6\u522b\u7ebf\u6027\u6c28\u57fa\u9178\u5e8f\u5217\u7684\u86cb\u767d\u9176\u4e0d\u540c\uff0cSUMO\u86cb\u767d\u9176\u8bc6\u522b\u5176\u5e95\u7269\u7684\u4e09\u7ea7\u7ed3\u6784\uff0c\u56e0\u6b64\u5177\u6709\u9ad8\u5ea6\u7279\u5f02\u6027\u3002SUMO\u5e95\u7269\u662f\u4e00\u4e2a\u901a\u5e38\u5728\u76ee\u7684\u86cb\u767dN\u7aef\u52a0\u5165\u7684\u7528\u4e8e\u63d0\u9ad8\u5176\u6eb6\u89e3\u6027\u548c\u8868\u8fbe\u768410kDa\u7684\u86cb\u767d\uff0c\u4e0eMBP\u6c34\u5e73\u76f8\u5f53\u3002\u4e0e\u5176\u4ed6\u86cb\u767d\u9176\u76f8\u6bd4\uff0c\u5b83\u7684\u4e00\u4e2a\u4e3b\u8981\u4f18\u52bf\u662f\u57282M\u5c3f\u7d20\u548c\u9ad8\u76d0\u6d53\u5ea6\u7684\u6761\u4ef6\u4e0b\u4f9d\u7136\u5177\u6709\u8f83\u9ad8\u7684\u9176\u5207\u6d3b\u6027\u3002SUMO\u7eaf\u5316\u7cfb\u7edf\u662f\u4e00\u4e2a\u5177\u6709\u9ad8\u6eb6\u89e3\u6027\u3001\u9ad8\u4f4d\u70b9\u9176\u5207\u7279\u5f02\u6027\u3001\u80fd\u4ea7\u751f\u5929\u7136N\u672b\u7aef\u7b49\u4f18\u52bf\u7684\u53ef\u9760\u7684\u86cb\u767d\u8d28\u8868\u8fbe\u4e0e\u7eaf\u5316\u5e73\u53f0\u3002\u4f46\u7f3a\u70b9\u662f\u6210\u672c\u76f8\u5bf9\u8f83\u9ad8\u3002<\/span><\/p>\n<p style=\"margin-bottom: 0in;\"><br  \/><\/p>\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\"  >\n<section class=\"135brush\"   style=\"color: rgb(5, 115, 175);padding: 0.45em 0.6em 0.45em 0.9em;border-left: 10px solid rgb(83, 191, 250);border-top-color: rgb(83, 191, 250);border-right-color: rgb(83, 191, 250);border-bottom-color: rgb(83, 191, 250);background: rgb(182, 228, 253);box-sizing: border-box;\">3.\u603b\u7ed3<\/section>\n<\/section>\n<\/section>\n<p style=\"text-indent: 0.29in;margin-bottom: 0in;\"><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">\u56de\u987e\u8fc7\u53bb\u51e0\u5341\u5e74\u4e3a\u8868\u8fbe\u548c\u7eaf\u5316\u91cd\u7ec4\u86cb\u767d\u800c\u5f00\u53d1\u7684\u4eb2\u548c\u6807\u7b7e\u7684\u53d1\u5c55\u8d8b\u52bf\uff0c\u4e3a\u4e86\u63d0\u9ad8\u7eaf\u5316\u6548\u7387\uff0c\u5df2\u7ecf\u5411\u4f7f\u7528IMAC\u8fdb\u884c\u4e86\u91cd\u5927\u8f6c\u79fb\u3002\u4ece2000\u5e74\u8d77\uff0c\u7531\u4e8e\u514d\u75ab\u4eb2\u548c\u6811\u8102\u7684\u6301\u7eed\u9ad8\u6210\u672c\u4ee5\u53ca\u5176\u4ed6\u7eaf\u5316\u7cfb\u7edf\u7684\u6539\u8fdb\uff0c\u4f7f\u7528\u8868\u4f4d\u6807\u7b7e\u7684\u7814\u7a76\u5df2\u7ecf\u5927\u5e45\u51cf\u5c11\u3002\u4f46\u5728\u9700\u8981\u8f7d\u4f53\u86cb\u767d\u4fc3\u8fdb\u9002\u5f53\u6298\u53e0\u3001\u589e\u52a0\u6eb6\u89e3\u6027\u548c\u63d0\u9ad8\u8868\u8fbe\u6c34\u5e73\u7684\u5e94\u7528\u4e2d\uff0c\u8fd9\u4e9b\u6807\u7b7e\u7684\u4f7f\u7528\u4ecd\u7136\u662f\u6709\u4ef7\u503c\u7684\uff0c\u6709\u65f6\u662f\u5fc5\u8981\u7684\u3002\u8003\u8651\u5230\u8fd9\u4e00\u9700\u8981\uff0c\u5728\u9009\u62e9\u86cb\u767d\u8d28\u7eaf\u5316\u7684\u4eb2\u548c\u6807\u7b7e\u548c\u53bb\u9664\u6807\u7b7e\u7684\u5de5\u5177\u65b9\u9762\u6709\u4e86\u8bb8\u591a\u4e0d\u540c\u7684\u65b9\u6cd5\u3002\u5728\u8fc7\u53bb15\u5e74\u4e2d\uff0c\u8f7d\u4f53\u86cb\u767d\u4e0e\u9ad8\u4eb2\u548c\u529b\u7ed3\u5408\u7684IMAC\u6807\u7b7e\u7684\u7ed3\u5408\u4f7f\u7528\u6709\u4e86\u8d8a\u6765\u8d8a\u591a\u7684\u8d8b\u52bf\u3002\u672a\u6765\u7684\u8d8b\u52bf\u662f\u4f7f\u7528\u4e0e\u9ad8\u53ef\u6eb6\u6027\u8f7d\u4f53\u86cb\u767d\u7684N\u7aef\u878d\u5408\u7684\u5c0f\u5206\u5b50\u7684\u4eb2\u548c\u529b\u6807\u7b7e\uff0c\u4fc3\u8fdb\u76ee\u7684\u86cb\u767d\u7684\u6298\u53e0\u3001\u6eb6\u89e3\u3001\u8868\u8fbe\u548c\u7eaf\u5316\uff0c\u5e76\u8fdb\u884c\u67f1\u4e0a\u7684\u7eaf\u5316\u53ca\u9176\u5207\u4f4d\u70b9\u7684\u53bb\u9664\u3002<\/span><\/p>\n<p style=\"text-align: center;\"><a href=\"https:\/\/mp.weixin.qq.com\/s?__biz=MjM5MjQxMDc3Ng==&amp;mid=2650852654&amp;idx=1&amp;sn=8709962e93650bfa350d58de7f3f0acf&amp;scene=21#wechat_redirect\" target=\"_blank\" ><span class=\"js_jump_icon h5_image_link\"  style=\"top: auto;left: auto;margin: 0px;right: auto;bottom: auto;\"><img class=\"rich_pages\"       style=\"margin: 0px;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/4-1558084242.jpg\" alt=\"\u4eb2\u548c\u6807\u7b7e\u5728\u91cd\u7ec4\u86cb\u767d\u8868\u8fbe\u4e0e\u7eaf\u5316\u7684\u4f7f\u7528\u53ca\u6bd4\u8f83\" title=\"\u4eb2\u548c\u6807\u7b7e\u5728\u91cd\u7ec4\u86cb\u767d\u8868\u8fbe\u4e0e\u7eaf\u5316\u7684\u4f7f\u7528\u53ca\u6bd4\u8f83\"  \/><\/span><\/a><\/p>\n<p style=\"margin-bottom: 0in;\"><strong><span style=\"font-size: 15px;font-family: \u5b8b\u4f53, SimSun;\">\u53c2\u8003\u6587\u732e\uff1a<\/span><\/strong><br  \/><\/p>\n<p style=\"margin-bottom: 0in;\"><span style=\"font-family: \u5b8b\u4f53, SimSun;font-size: 10px;\">\u30101\u3011C.Amarasinghe, J.P. Jin, The Use of Affinity Tags to Overcome Obstaclesin Recombinant Protein Expression and Purification, Protein PeptideLett, 22 (2015) 885-892.<\/span><\/p>\n<p style=\"margin-bottom: 0in;\"><span style=\"font-family: \u5b8b\u4f53, SimSun;font-size: 10px;\">\u30102\u3011J.Arnau, C. Lauritzen, G.E. Petersen, J. Pedersen, Current strategiesfor the use of affinity tags and tag removal for the purification ofrecombinant proteins, Protein Expres Purif, 48 (2006) 1-13.<\/span><\/p>\n<\/section>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;white-space: normal;font-family: \u5fae\u8f6f\u96c5\u9ed1;background-color: rgb(255, 255, 255);text-align: center;letter-spacing: 0.54px;line-height: normal;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><strong style=\"max-width: 100%;color: rgb(255, 41, 65);line-height: 25.6px;font-size: 18px;box-sizing: border-box !important;overflow-wrap: break-word !important;\">\u8bc6\u522b\u5fae\u4fe1\u4e8c\u7ef4\u7801\uff0c\u6dfb\u52a0\u5c0f\u7f16\uff0c\u7b26\u5408\u6761\u4ef6\u8005\u5373\u53ef\u52a0\u5165\u751f\u7269\u5236\u54c1\u5fae\u4fe1\u7fa4\uff01<\/strong><\/p>\n<section label=\"Powered by 135editor.com\"  style=\"max-width: 100%;font-size: 16px;white-space: normal;font-family: \u5fae\u8f6f\u96c5\u9ed1;background-color: rgb(255, 255, 255);letter-spacing: 0.54px;box-sizing: border-box !important;overflow-wrap: break-word !important;\">\n<section label=\"Powered by 135editor.com\"  style=\"max-width: 100%;line-height: 25.6px;box-sizing: border-box !important;overflow-wrap: break-word !important;\">\n<p style=\"max-width: 100%;min-height: 1em;text-align: center;line-height: 25.6px;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><span style=\"max-width: 100%;color: rgb(0, 128, 255);font-size: 20px;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\">\u8bf7\u6ce8\u660e\uff1a\u59d3\u540d+\u7814\u7a76\u65b9\u5411\uff01<\/strong><\/span><\/p>\n<section label=\"Powered by 135editor.com\"  style=\"max-width: 100%;letter-spacing: 0.54px;box-sizing: border-box !important;overflow-wrap: break-word !important;\">\n<section label=\"Powered by 135editor.com\"  style=\"max-width: 100%;line-height: 25.6px;box-sizing: border-box !important;overflow-wrap: break-word !important;\">\n<p style=\"max-width: 100%;min-height: 1em;text-align: center;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><img class=\"\"       style=\"box-sizing: border-box !important;overflow-wrap: break-word !important;visibility: visible !important;width: 253px !important;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/6-1558084242.jpeg\" alt=\"\u4eb2\u548c\u6807\u7b7e\u5728\u91cd\u7ec4\u86cb\u767d\u8868\u8fbe\u4e0e\u7eaf\u5316\u7684\u4f7f\u7528\u53ca\u6bd4\u8f83\" title=\"\u4eb2\u548c\u6807\u7b7e\u5728\u91cd\u7ec4\u86cb\u767d\u8868\u8fbe\u4e0e\u7eaf\u5316\u7684\u4f7f\u7528\u53ca\u6bd4\u8f83\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;text-align: center;box-sizing: border-box !important;overflow-wrap: break-word !important;\"><img width=\"555\"    class=\"\"  style=\"widows: 1;letter-spacing: 0.54px;line-height: 25.6px;text-indent: 0em;vertical-align: middle;box-sizing: border-box !important;overflow-wrap: break-word !important;visibility: visible !important;max-height: 1.2em !important;width: 640px !important;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/8-1558084242.png\" alt=\"\u4eb2\u548c\u6807\u7b7e\u5728\u91cd\u7ec4\u86cb\u767d\u8868\u8fbe\u4e0e\u7eaf\u5316\u7684\u4f7f\u7528\u53ca\u6bd4\u8f83\" title=\"\u4eb2\u548c\u6807\u7b7e\u5728\u91cd\u7ec4\u86cb\u767d\u8868\u8fbe\u4e0e\u7eaf\u5316\u7684\u4f7f\u7528\u53ca\u6bd4\u8f83\"  \/><br style=\"max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\"  \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"max-width: 100%;font-size: 16px;letter-spacing: 0.544px;white-space: normal;font-family: \u5fae\u8f6f\u96c5\u9ed1;line-height: 25.6px;background-color: rgb(255, 255, 255);box-sizing: border-box !important;overflow-wrap: break-word !important;\">\n<section style=\"margin-top: 0.5em;margin-bottom: 0.5em;max-width: 100%;text-align: center;box-sizing: border-box !important;overflow-wrap: break-word !important;\">\n<section style=\"margin: -0.9em auto 0.9em;max-width: 100%;width: 0.6em;height: 0.6em;box-sizing: border-box !important;overflow-wrap: break-word !important;\">\n<section style=\"margin-left: 1px;max-width: 100%;border-radius: 100%;width: 9.59px;height: 9.59px;background-color: rgb(170, 33, 94);box-sizing: border-box !important;overflow-wrap: break-word !important;\"><br  style=\"max-width: 100%;box-sizing: border-box !important;overflow-wrap: break-word !important;\"  \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"max-width: 100%;letter-spacing: 0.544px;white-space: normal;font-family: \u5fae\u8f6f\u96c5\u9ed1;line-height: 25.6px;font-size: 14px;background-color: rgb(255, 255, 255);box-sizing: border-box !important;overflow-wrap: break-word !important;\">\n<section style=\"margin-top: 10px;margin-bottom: 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