{"id":107798,"date":"2019-05-09T00:00:50","date_gmt":"2019-05-17T09:19:04","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/107798\/"},"modified":"2019-05-17T17:19:06","modified_gmt":"2019-05-17T09:19:06","slug":"eddb5abc9c","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/107798\/","title":{"rendered":"\u4e0d\u540cCHO\u7ec6\u80de\u7cfb\u5728\u6297\u4f53\u8868\u8fbe\u4e0e\u751f\u7269\u91cf\u5408\u6210\u4e4b\u95f4\u7684\u6289\u62e9"},"content":{"rendered":"
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1<\/section>\n
\u80cc\u666f\u4ecb\u7ecd<\/section>\n<\/section>\n<\/section>\n

\u4e2d\u56fd\u4ed3\u9f20\u5375\u5de2\u7ec6\u80deCHO\u5df2\u5f00\u53d1\u51fa\u4e86CHO-DXB11,CHO-K1, CHO-DG44, \u548cCHO-S\u7b49\u4e0d\u540c\u8c31\u7cfb\u7684\u7ec6\u80de\u7cfb\u3002\u5c3d\u7ba1\u6240\u6709\u7684\u7ec6\u80de\u7cfb\u90fd\u51fa\u81ea\u4e00\u4e2a\u7956\u5148\uff0c\u4f46\u662f\u5e7f\u6cdb\u7684\u57fa\u56e0\u7a81\u53d8\uff0c\u514b\u9686\u7b5b\u9009\u4f7f\u5f97\u5b83\u4eec\u4e4b\u95f4\u5df2\u6709\u4e86\u5927\u91cf\u7684\u5f02\u8d28\u6027\u3002\u6d4b\u5e8f\u6570\u636e\u663e\u793a\u5b83\u4eec\u6709\u7740\u4e0d\u540c\u7684\u57fa\u56e0\u7f3a\u5931\u548c\u7a81\u53d8\uff0c\u540c\u65f6\u4e5f\u6709\u4e86\u4e0d\u540c\u7684\u751f\u7269\u8fdb\u7a0b\u3002\u9c9c\u6709\u6587\u7ae0\u62a5\u9053\u7531\u4e8eCHO\u7ec6\u80de\u7cfb\u57fa\u56e0\u5dee\u5f02\u9020\u6210\u7684\u751f\u7269\u8fdb\u7a0b\u7684\u4e0d\u540c\u3002\u672c\u6587\u4ee5\u4eba\u5de5\u67d3\u8272\u4f53BAC\u4e3a\u8f7d\u4f53\uff0c\u63a2\u7a76\u4e863\u79cd\u6700\u5e38\u7528\u7ec6\u80de\u7cfbCHO-K1,CHO-S, \u548cCHO-DG44\u5728\u8868\u8fbe\u57fa\u56e0\u5de5\u7a0b\u6297\u4f53\u65b9\u9762\u7684\u5dee\u5f02\u3002\u901a\u8fc7\u5206\u6279\u57f9\u517b\uff0c\u5206\u6279\u8865\u6599\u57f9\u517b\uff0c\u534a\u8fde\u7eed\u704c\u6ce8\u57f9\u517b\u7684\u65b9\u5f0f\u7814\u7a76\u4e86\u7ec6\u80de\u7279\u5f02\u6027\u589e\u6b96\u4ee5\u53ca\u4ea7\u7269\u5f62\u6210\u60c5\u51b5\u3002\u9664\u6b64\u4e4b\u5916\uff0c\u672c\u6587\u8fd8\u7814\u7a76\u4e86\u4e24\u79cd\u4e0d\u540c\u57f9\u517b\u57fa\u5bf9\u6297\u4f53\u8868\u8fbe\u91cf\u548c\u7cd6\u57fa\u5316\u7684\u5f71\u54cd\u3002\u6211\u4eec\u53d1\u73b0\uff0cCHO\u7ec6\u80de\u7279\u5f02\u6027\u5408\u6210\u6297\u4f53\u7684\u80fd\u529b\u5c5e\u4e8e\u4e0d\u540c\u7ec6\u80de\u7cfb\u7684\u4e13\u6709\u5c5e\u6027\uff0c\u5bbf\u4e3b\u7ec6\u80de\u548c\u57f9\u517b\u57fa\u5171\u540c\u51b3\u5b9a\u4e86\u6297\u4f53\u7684\u4ea7\u91cf\u548c\u8d28\u91cf\u3002<\/span><\/p>\n

CHO-DXB11,CHO-K1, CHO-DG44, \u548cCHO-S\u59cb\u4e8e\u540c\u4e00\u4e2a\u7956\u5148\uff0cCHO\u7531Dr.Theodore Puck\u4e8e1956\u5e74\u5206\u79bb\u5f97\u5230\uff0cCHO-K1\u4f5c\u4e3aCHO\u7684\u4e9a\u514b\u9686\u682a\u8bde\u751f\u4e8e1957\u5e74\uff0c1980\u5e74\uff0cCHO-DXB11\u7531CHO-K1\u901a\u8fc7\u57fa\u56e0\u7a81\u53d8\u800c\u5f97\u5230\uff0c1983\u5e74Urlaub\u7b49\u4eba\u901a\u8fc7\u57fa\u56e0\u7a81\u53d8\u5220\u9664\u4e86DHFR\u53cc\u7b49\u4f4d\u57fa\u56e0\u5f97\u5230\u4e86CHO-DG44\uff0c1991\u5e74\u7531CHO\u6d3e\u751f\u51fa\u4e86CHO-S,\u622a\u81f3\u76ee\u524d\uff0c\u4ee5\u4e0a\u56db\u79cd\u7ec6\u80de\u7cfb\u90fd\u5df2\u7528\u4e8e\u5546\u4e1a\u5316\u533b\u836f\u751f\u4ea7\u3002<\/span><\/p>\n

\"\u4e0d\u540cCHO\u7ec6\u80de\u7cfb\u5728\u6297\u4f53\u8868\u8fbe\u4e0e\u751f\u7269\u91cf\u5408\u6210\u4e4b\u95f4\u7684\u6289\u62e9\"<\/p>\n

\u56fe1.CHO\u7ec6\u80de\u7cfb\u7a81\u53d8\u6982\u89c8<\/span><\/p>\n

\u56fe\u7247\u6765\u81eaNat.Biotechnol. 2013, 31, 759.<\/span><\/p>\n

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2<\/section>\n
\u7ed3\u679c\u4e0e\u5206\u6790<\/section>\n<\/section>\n<\/section>\n

2.1<\/strong>\u5236\u5907\u91cd\u7ec4\u6297\u4f53\u8868\u8fbe\u7ec6\u80de\u6c60\u53ca\u7ec6\u80de\u9002\u5e94\u6027\u57f9\u517b<\/strong><\/span><\/p>\n

\u5982\u8865\u5145\u56fe1A\u3001B\u6240\u793a\u521d\u6b21BAC\u8f6c\u67d3\u7684CHO-K1,CHO-S, \u548cCHO-DG443\u79cd\u7ec6\u80de\u7cfb\uff0c\u5bf9\u5e94\u7684\u7ec6\u80de\u6c60\u5206\u6ccc\u6297\u4f53\u7684\u80fd\u529b\u5206\u522b\u4e3a5.04\u00b12.37pg\/\/Day(CHO-K1), 3.29\u00b11.82 pg\/\/Day(CHO-S), 1.10 \u00b10.73pg\/\/Day(CHO-DG44) \uff0c\u7ec6\u80de\u8f6c\u67d3\uff0c\u7b5b\u9009\uff0c\u4e9a\u514b\u9686\u90fd\u5728CDCHO\u57f9\u517b\u57fa\u4e2d\u5b8c\u6210\uff0c\u7ec6\u80de\u9002\u5e94\u6027\u57f9\u517b\u5728ActiCHOTMP (GE Healthcare) \u57f9\u517b\u57fa\u4e2d\u8fdb\u884c\u3002\u6d41\u5f0f\u7ec6\u80de\u672f\u68c0\u6d4b\u4e09\u79cd\u7ec6\u80de\u7cfb\u6784\u5efa\u7684\u91cd\u7ec4\u7ec6\u80de\u682a\u90fd\u6709\u5f88\u597d\u7684\u9002\u5e94\u6027\uff0c\u7ec6\u80de\u5bc6\u5ea6\u90fd\u80fd\u5f88\u597d\u7684\u8fbe\u5230CDCHO\u57f9\u517b\u57fa\u57f9\u517b\u7684\u5bc6\u5ea6\uff0c\u751a\u81f3\u66f4\u9ad8\uff0c\u4e14\u51e0\u4e4e\u6ca1\u6709\u6297\u4f53\u8868\u8fbe\u4e22\u5931\u7684\u7ec6\u80de\u3002<\/span><\/p>\n

\"\u4e0d\u540cCHO\u7ec6\u80de\u7cfb\u5728\u6297\u4f53\u8868\u8fbe\u4e0e\u751f\u7269\u91cf\u5408\u6210\u4e4b\u95f4\u7684\u6289\u62e9\"<\/p>\n

\u8865\u5145\u56fe<\/strong>1.<\/strong>\u4e09\u79cd\u7ec6\u80de\u7cfb\u7531<\/strong>CDCHO<\/strong>\u57f9\u517b\u57fa\u5230<\/strong>ActiCHO<\/strong>TM<\/strong><\/sup>P<\/strong>\u57f9\u517b\u57fa\u7684\u9002\u5e94\u8fc7\u7a0b\u3002<\/strong><\/span><\/p>\n

2.2<\/strong>\u5206\u6279\u57f9\u517b\u8bc4\u4f30<\/strong>3<\/strong>\u79cd\u7ec6\u80de\u7cfb\u7684\u751f\u4ea7\u6027\u80fd<\/strong><\/span><\/p>\n

\u5982\u56fe1\u6240\u793a\u5206\u6279\u57f9\u517b\u671f\u95f4\u4e0eCHO-S,\u548cCHO-DG44\u76f8\u6bd4CHO-K1\u5728ActiCHOTMP\u57f9\u517b\u57fa\u4e2d\u8868\u73b0\u51fa\u4e864-6\u500d\u7684\u7ec6\u80de\u5bc6\u5ea6\u548c\u6297\u4f53\u8868\u8fbe\u91cf\u589e\u957f\uff0c\u56e0\u6b64\u5728\u6279\u91cf\u57f9\u517b\u671f\u95f4CHO-K1\u663e\u793a\u51fa\u6700\u4f73\u7684\u6297\u4f53\u751f\u4ea7\u6027\u80fd\uff0cCHO-S\u6700\u5dee\uff0c\u7ec6\u80de\u5b58\u6d3b\u65f6\u95f4\u548c\u7ec6\u80de\u5bc6\u5ea6\u4e0e\u7ec6\u80de\u6297\u4f53\u4ea7\u91cf\u5173\u7cfb\u5206\u6790\u663e\u793aCHO-S\u8868\u73b0\u51fa\u4e86\u6700\u5feb\u7684\u589e\u6b96\u901f\u5ea6\uff0cCHO-K1\u8868\u73b0\u51fa\u4e86\u6700\u597d\u7684\u6297\u4f53\u8868\u8fbe\u91cf\u3002<\/span><\/p>\n

2.3<\/strong>\u6279\u91cf\u8865\u6599\u57f9\u517b\u8bc4\u4f30<\/strong>3<\/strong>\u79cd\u7ec6\u80de\u7cfb\u7684\u751f\u4ea7\u6027\u80fd<\/strong><\/span><\/p>\n

\u5728ActiCHOTM<\/sup>P\u57f9\u517b\u57fa\u6279\u91cf\u8865\u6599\u57f9\u517b\u6761\u4ef6\u4e0bCHO-S\u4f9d\u7136\u8868\u73b0\u51fa\u4e86\u6700\u4f73\u7684\u589e\u6b96\u901f\u5ea6\uff0c\u6297\u4f53\u8868\u8fbe\u65b9\u9762CHO-DG44\u6700\u4f73\u8fbe\u5230\u4e86670mg .L-1<\/sup>\uff0cCHO-K1\u6b21\u4e4b\uff0c\u8fbe\u5230\u4e86460mg.L-1<\/sup>\uff0cCHO-S370 mg .L-1<\/sup>\u3002\u6709\u8da3\u7684\u662f\u5728CDCHO\u57f9\u517b\u57fa\u5206\u6279\u8865\u6599\u57f9\u517b\u6761\u4ef6\u4e0bCHO-K1\u4ea7\u91cf\u4e3a350mg.L-1<\/sup>\uff0cCHO-S\u4e3a175mg.L-1<\/sup>\uff0c\u800cCHO-DG44\u4ec5\u4e3a115mg.L-1<\/sup>\u3002\u4ee5\u4e0a\u5b9e\u9a8c\u7ed3\u679c\u8868\u660e\u6dfb\u52a0\u8425\u517b\u6db2\u6d53\u7f29\u7269\u663e\u8457\u5f71\u54cd\u7740\u90a3\u4e9b\u5bf9\u6240\u6dfb\u52a0\u7269\u9a97\u53f7\u7684\u5bbf\u4e3b\u7ec6\u80de\u7684\u7d2f\u8ba1\u3002\u4f46\u662f\u65e0\u8bba\u5982\u4f55CHO-K1\u4fdd\u6301\u4e86\u6297\u4f53\u7684\u9ad8\u6c34\u5e73\u8868\u8fbe\uff0cCHO-DG44\uff0cCHO-S\u6b21\u4e4b\u3002<\/span><\/p>\n

\u5bf9\u6bd4\u5206\u6279\u57f9\u517b\uff0cActiCHOTM<\/sup>P\u57f9\u517b\u57fa\u4e2dCHO-K1\u7ec6\u80de\u5bc6\u5ea6\u6709\u4e2a\u8fd1\u4e4e2\u500d\u7684\u5cf0\u503c\uff0c\u4f46\u6297\u4f53\u7684\u4ea7\u91cf\u4ec5\u9ad8\u51fa10%-20%\uff0c\u5176\u539f\u56e0\u5f52\u548e\u4e8e\u6297\u4f53\u4ea7\u751f\u7387\u7684\u663e\u8457\u964d\u4f4e\u3002\u8865\u6599\u5206\u6279\u57f9\u517b\u6ca1\u6709\u5f71\u54cdCHO-S\u7684\u7ec6\u80de\u5bc6\u5ea6\u5cf0\u503c\uff0c\u4f46\u662f\u968f\u7740\u6301\u7eed\u57f9\u517b\u5230\u7b2c6\u5929\uff0c\u53ef\u884c\u7684\u7d2f\u79ef\u57f9\u517b\u5929\u6570\u7684\u5ef6\u957f\u51e0\u4e4e\u4f7f\u5f97\u6297\u4f53\u6d53\u5ea6\u589e\u52a0\u4e866\u500d\uff0c\u5728CDCHO\u7ec6\u80de\u57f9\u517b\u57fa\u4e2d\uff0c\u5206\u6279\u8865\u6599\u57f9\u517bCHO-S\u7ec6\u80de\u5176\u53ef\u884c\u6027\u7ec6\u80de\u7d2f\u79ef\u5929\u6570\u4e0d\u53ca\u5728ActiCHOTM<\/sup>P\u57f9\u517b\u57fa\u4e2d\uff0c\u56e0\u6b64\u5728\u5206\u6279\u8865\u6599\u57f9\u517b\u8fc7\u7a0b\u4e2d\uff0c\u6297\u4f53\u8868\u8fbe\u589e\u52a0\u4ec52.5\u500d\u3002\u5bf9\u4e8eCHO-DG44\u6765\u8bf4\uff0c\u5206\u6279\u8865\u6599\u57f9\u517b\u4f7f\u5f97\u7ec6\u80de\u5bc6\u5ea6\u4e5f\u6709\u4e2a\u8fd1\u4e4e2\u500d\u7684\u5cf0\u503c\uff0c\u7ec6\u80de\u53ef\u884c\u6027\u7d2f\u79ef\u65f6\u95f4\u548c\u7279\u5f02\u6027\u7ec6\u80de\u6297\u4f53\u4ea7\u751f\u7387\u4e5f\u6709\u63d0\u9ad8\uff0c\u6297\u4f53\u603b\u8868\u8fbe\u91cf\u63d0\u9ad8\u4e86\u8fd1\u4e4e5\u500d\uff0c\u4f46\u662fCDCHO\u8865\u6599\u5206\u6279\u57f9\u517b\u5e76\u6ca1\u6709\u6539\u5584\u7ec6\u80de\u7684\u5bc6\u5ea6\u548c\u6297\u4f53\u8868\u8fbe\u80fd\u529b\u3002<\/span><\/p>\n

2.4<\/strong>\u8fde\u7eed\u704c\u6ce8\u57f9\u517b\u8bc4\u4f30<\/strong>3<\/strong>\u79cd\u7ec6\u80de\u7cfb\u7684\u751f\u4ea7\u6027\u80fd<\/strong><\/span><\/p>\n

\u534a\u8fde\u7eed\u704c\u6ce8\u57f9\u517b\u8fc7\u7a0b\u4e2d\uff0c\u6bcf\u5929\u6362\u6db2\u4e00\u6b21\uff0cCHO-S\u7ec6\u80de\u8868\u73b0\u51fa\u6700\u5feb\u7684\u589e\u6b96\u901f\u5ea6\uff0c\u6700\u5148\u8fbe\u5230\u7ec6\u80de\u5bc6\u5ea6\u5cf0\u503c\uff0cCHO-K1\u4ee5\u6700\u4f4e\u7684\u7ec6\u80de\u5bc6\u5ea625×106 c ml-1<\/sup>\u5230\u8fbe\u7a33\u5b9a\u671f\uff0c\u8fd9\u4e00\u503c\u4e0eCHO-DG44\u5728CDCHO\u57f9\u517b\u57fa\u4e2d\u7684\u503c\u7c7b\u4f3c\u3002\u6709\u8da3\u7684\u662fCHO-DG44\u5728ActiCHOTM<\/sup>P\u57f9\u517b\u57fa\u4e2d\u7ec6\u80de\u6301\u7eed\u6027\u589e\u503c\uff0c\u7ec6\u80de\u5bc6\u5ea6\u8fbe\u5230\u4e86CHO\u5728\u5404\u79cd\u57f9\u517b\u6761\u4ef6\u4e0b\u7684\u6700\u5927\u503c\uff0c\u8fd9\u4e00\u7ed3\u679c\u8bc1\u660e\u4e86\u6bcf\u5929\u6362\u6db2\u4fdd\u8bc1\u4e86CHO\u4e0d\u540c\u8c31\u7cfb\u7ec6\u80de\u5404\u81ea\u7684\u8425\u517b\u9700\u6c42\uff0c\u4ece\u800c\u6781\u5927\u5f71\u54cd\u7740\u7ec6\u80de\u7684\u589e\u6b96\u3002\u6bcf\u969424h\u6362\u6db2\u4e00\u6b21CHO-K1\u8868\u73b0\u51fa\u6700\u9ad8\u7684\u6297\u4f53\u8868\u8fbe\u91cf\uff0cCHO-DG44\uff0cCHO-S\u6b21\u4e4b\u3002\u5728\u4e24\u79cd\u57f9\u517b\u57fa\u4e2dCHO-K1\u90fd\u8868\u73b0\u51fa\u4e86\u6700\u9ad8\u7684\u6297\u4f53\u751f\u4ea7\u738713-16pg\/\/Day\uff0c\u8fdc\u8d85CHO-DG444-5pg\/\/Day\uff0cCHO-S2pg\/\/Day\u3002<\/span><\/p>\n

\"\u4e0d\u540cCHO\u7ec6\u80de\u7cfb\u5728\u6297\u4f53\u8868\u8fbe\u4e0e\u751f\u7269\u91cf\u5408\u6210\u4e4b\u95f4\u7684\u6289\u62e9\"<\/p>\n

\u56fe1.\u603b\u7ec6\u80de\u5bc6\u5ea6\u548c\u53ef\u884c\u6027\u7d2f\u8ba1\u57f9\u517b\u65f6\u95f4\u5bf9\u5e94\u4e09\u79cd\u7ec6\u80de\u7cfbCHO-K1,CHO-DG44,CHO-S\u5728\u4e09\u79cd\u57f9\u517b\u6761\u4ef6\uff0c\u6279\u91cf\uff0c\u8865\u6599\u6279\u91cf\uff0c\u704c\u6ce8\u57f9\u517b\uff0c\u4e24\u79cd\u57f9\u517b\u57faCDCHO,ActiCHOTM<\/sup>\u57f9\u517b\u6761\u4ef6\u4e0b\u6297\u4f53\u8868\u8fbe\u60c5\u51b5\u3002<\/span><\/p>\n

2.5mRNA<\/strong>\u8bc4\u4f30<\/strong><\/span><\/p>\n

\u9ad8\u6c34\u5e73\u6297\u4f53\u751f\u4ea7\u7387\u662f\u5426\u610f\u5473\u7740\u9ad8\u6c34\u5e73\u7684\u8f7b\u91cd\u94femRNA,\u901a\u8fc7\u5bf9\u8865\u6599\u5206\u6279\u57f9\u517b\u7ec6\u80de\u51e0\u5929\u540e\uff0c\u5bf9\u6297\u4f53\u91cd\u94fe\u8f7b\u94femRNA\u5b9a\u91cf\u5206\u6790\uff0c\u53d1\u73b0\u4e09\u79cdCHO\u7ec6\u80de\u7cfb\uff0c\u968f\u7740\u57f9\u517b\u65f6\u95f4\u7684\u7d2f\u79ef\uff0c\u7ec6\u80de\u7279\u5f02\u6027\u6297\u4f53\u751f\u4ea7\u7387\u9010\u6e10\u964d\u4f4e\uff0c\u91cd\u94fe\uff0c\u8f7b\u94femRNA\u4e5f\u5b58\u5728\u7740\u76f8\u5e94\u7684\u5dee\u5f02\u3002\u56e0\u6b64\u6211\u4eec\u7edf\u8ba1\u4e86\u57f9\u517b\u7b2c4\u5929\u4ea7\u7269\u7279\u5f02\u6027mRNA\u548c\u7ec6\u80de\u7279\u5f02\u6027\u6297\u4f53\u751f\u4ea7\u529bqP\u503c\uff0c\u6240\u6709\u7684\u7ec6\u80de\u514b\u9686\u5904\u4e8e\u76f8\u540c\u7684\u7ec6\u80de\u5bc6\u5ea6\u6c34\u5e73\uff0c\u90fd\u5904\u4e8e\u6307\u6570\u751f\u957f\u671f\u3002\u6709\u8da3\u7684\u662fCHO-K1\u7ec6\u80de\u91cc\u6297\u4f53\u91cd\u94fe\u8f7b\u94femRNA\u6c34\u5e73\u4e0e\u8865\u6599\u606f\u606f\u76f8\u5173\uff0c\u76f8\u5e94\u7684\u8fd9\u79cd\u8865\u6599\u4e5f\u5f71\u54cd\u7740\u7279\u5f02\u6027\u6297\u4f53\u8868\u8fbe\u91cf\u3002\u4f46\u662f\u7edf\u8ba1\u6570\u636e\u663e\u793amRNA\u6c34\u5e73\u65e0\u6cd5\u51c6\u786e\u9884\u6d4b\u6297\u4f53\u8868\u8fbe\u91cf\u3002<\/span><\/p>\n

\"\u4e0d\u540cCHO\u7ec6\u80de\u7cfb\u5728\u6297\u4f53\u8868\u8fbe\u4e0e\u751f\u7269\u91cf\u5408\u6210\u4e4b\u95f4\u7684\u6289\u62e9\"<\/p>\n


<\/p>\n

\u56fe2.\u5e73\u5747\u6c34\u5e73\uff08A\uff09\u91cd\u94femRNA,(B)\u8f7b\u94femRNA,(C)\u704c\u6ce8\u57f9\u517b\u8fc7\u7a0b\u4e2d\u4e09\u79cd\u7ec6\u80de\u7cfb\u5728\u4e24\u79cd\u57f9\u517b\u57fa\u91cc\u7b2c4,7\u53ca\u7ec8\u6b62\u57f9\u517b\u65f6\u6297\u4f53\u4ea7\u7387\u3002<\/span><\/strong><\/p>\n

2.6CHO<\/strong>\u7ec6\u80de\u7cfb\u548c\u6297\u4f53\u751f\u4ea7\u8d28\u91cf<\/strong><\/span><\/p>\n

\u57f9\u517b\u8fc7\u7a0b\u4e2d\u4e0d\u540cCHO\u7ec6\u80de\u7cfb\u8868\u73b0\u51fa\u7684\u5dee\u5f02\u63d0\u9192\u6211\u4eec\u4e0d\u5f97\u4e0d\u53bb\u8003\u91cf\u6240\u4ea7\u6297\u4f53\u7684\u8d28\u91cf\u3002\u6211\u4eec\u51b3\u5b9a\u9996\u5148\u4ece\u8865\u6599\u5206\u6279\u57f9\u517b\u5f00\u59cb\u7814\u7a76\uff0c\u56e0\u4e3a\u76ee\u524d\u591a\u6570\u751f\u4ea7\u5de5\u827a\u90fd\u91c7\u53d6\u8865\u6599\u5206\u6279\u57f9\u517b\u7684\u7b56\u7565\uff0c\u6211\u4eec\u63a8\u6d4b\uff0c\u7531\u4e8e\u8865\u6599\u6279\u6b21\u7684\u4ea7\u54c1\u79ef\u7d2f\uff0c\u5373\u4f7f\u662f\u5fae\u5c0f\u7684\u7ec6\u80de\u7cfb\uff0c\u7279\u522b\u662f\u4e0e\u4ea7\u54c1\u76f8\u5173\u7684\u5dee\u5f02\u53ef\u80fd\u6bd4\u5176\u4ed6\u5de5\u827a\u6a21\u5f0f\u66f4\u660e\u663e\u3002<\/span><\/p>\n

\u603b\u4f53\u4e0a\u6765\u8bf4\u4e09\u79cd\u7ec6\u80de\u7cfb\u8868\u8fbe\u6297\u4f53\u7684Fc\u6bb5\u5ca9\u85fb\u7cd6\u7cd6\u57fa\u5316\u4e3b\u8981\u6709G0F,G1F, \u548cG2F\u4e09\u79cd\u5f62\u5f0f\uff0c\u7c7b\u4f3c\u4e8e\u81ea\u7136\u72b6\u6001\u4e0b\u4eba\u8840\u6e05\u4e2dIgG\u7684\u5f62\u5f0f\u3002\u7814\u7a76\u8868\u660e\u7ec6\u80de\u7cfb\u548c\u57f9\u517b\u57fa\u5f71\u54cd\u7740\u67d0\u4e9b\u5f62\u5f0f\u7684\u7cd6\u76f8\u5bf9\u4e30\u5ea6\u3002\u6bd4\u5982\u5f53\u57f9\u517b\u57fa\u4eceActiCHOTM<\/sup>P\u8f6c\u6362\u4e3aCDCHO\u57f9\u517b\u57fa\u65f6\uff0cCHO-K1\u548cCHO-S\u7ec6\u80de\u4e2d\u6297\u4f53\u534a\u4e73\u7cd6\u57fa\u5316\u542b\u91cf\u4ece17%\u63d0\u5347\u523037%\uff0c\u800cCHO-DG44\u4e2d\u534a\u4e73\u7cd6\u7cd6\u57fa\u5316\u6297\u4f53\u542b\u91cf\u4e0d\u53d8\u3002\u8868\u8fbe\u7684\u5355\u6297\u4e0aG0F\uff08\u65e0\u4e73\u7cd6\u9170\u5316\u7cd6\uff09\u7684\u4e30\u5ea6\u5728CHO-S\u7ec4\u9ad8\u4e8eCHO-K1\u548cCHO-DG44\u3002\u4ea7\u7269\u7518\u9732\u7cd6\u7cd6\u57fa\u5316\uff0c\u5ca9\u85fb\u7cd6\u7cd6\u57fa\u5316\uff0c\u65e0\u4e73\u7cd6\u7cd6\u57fa\u5316\u51e0\u4e4e\u662f\u4e0d\u540cCHO\u7ec6\u80de\u7684\u4e13\u6709\u5c5e\u6027\uff0c\u57f9\u517b\u57fa\u5bf9\u5176\u5f71\u54cd\u4e0d\u5927\u3002CHO-S\u7ec4\u8868\u8fbe\u4ea7\u7269\u7518\u9732\u7cd6\u7cd6\u57fa\u5316\u6c34\u5e73\u57282%-3%\uff0c\u800cCHO-K1\u548cCHO-DG44\u4ea7\u7269\u7518\u9732\u7cd6\u7cd6\u57fa\u5316\u6c34\u5e73\u5206\u522b\u4e3a5%-9%,11%-13%\u3002\u4ea7\u7269\u5ca9\u85fb\u7cd6\u7cd6\u57fa\u5316\u5728CHO-S\u7ec6\u80de\u91cc\u9ad8\u8fbe94%-96%\uff0c\u5728CHO-K1\u548cCHO-DG44\u5206\u522b\u4e3a82%\u201384%\u548c71%\u201383%\u3002\u65e0\u4e73\u7cd6\u7cd6\u578b\u5728CHO-K1\u7ec6\u80de\u4ea7\u7269\u91cc\u53602%-5%\uff0cCHO-S\u7ec6\u80de\u91cc\u53601%\uff0cCHO-DG44\u7ec6\u80de\u4ea7\u7269\u91cc\u672a\u89c1\u5176\u5f62\u5f0f\u7684\u7cd6\u3002<\/span><\/p>\n

\"\u4e0d\u540cCHO\u7ec6\u80de\u7cfb\u5728\u6297\u4f53\u8868\u8fbe\u4e0e\u751f\u7269\u91cf\u5408\u6210\u4e4b\u95f4\u7684\u6289\u62e9\"<\/p>\n

\u56fe<\/strong>3.<\/strong>\u4e09\u79cd\u7ec6\u80de\u7cfb\u5728\u4e24\u79cd\u57f9\u517b\u57fa\u91cc\u704c\u6ce8\u57f9\u517b\u6761\u4ef6\u4e0b\u6297\u4f53\u91cd\u94fe\u7cd6\u578b\u5206\u5e03<\/strong><\/span><\/p>\n

\u9664\u4e86Fc\u4e0a\u7684\u7cd6\u57fa\u5316\u4f4d\u70b9\uff0c\u672c\u6587\u8fd8\u53d1\u73b0\u4e86\u8868\u8fbe\u6297\u4f53\u5728\u8f7b\u94fe\u4e0a\u8fd8\u5b58\u5728\u7740\u4e00\u4e2a\u7cd6\u57fa\u5316\u4f4d\u70b9\u3002\u8be5\u7cd6\u57fa\u5316\u4f4d\u70b9\u7cd6\u578b\u4e3b\u8981\u4ee5\u5ca9\u85fb\u7cd6\u4e3a\u6838\u5fc3\u7684\u7cd6\u590d\u5408\u7269\uff0c\u4ee5G0F,G1F,G2F\u5f62\u5f0f\u4e3a\u4e3b\uff0c\u5076\u5c14\u6709\u553e\u6db2\u9178\u6b8b\u57fa\u3002\u6297\u4f53\u8f7b\u94fe\u7518\u9732\u7cd6\u7cd6\u57fa\u5316\u5728CHO-S\u4ea7\u7269\u4e2d\u22641.5%\uff0c\u5728CHO-K1\u4e2d\u4e3a4%\u20136%\uff0cCHO-DG44\u4e3a5%-13%\u3002\u4ea7\u7269\u8f7b\u94fe\u5ca9\u85fb\u7cd6\u7cd6\u57fa\u5316\u5728CHO-S\u7ea6\u4e3a46%\uff0cCHODG44\u7ec6\u80de\u4e2d\u4e3a(76\u201388%)\uff0cCHO-K1\u7ec6\u80de\u4e2d\u4e3a83%\u201390%\u3002\u553e\u6db2\u9178\u5316\u6297\u4f53\u5728CHO-K1\u4e2d\u536021%-36%\uff0cCHO-DG44\u4e2d\u4e3a15%-19%\uff0cCHO-S\u4e2d\u4e3a6%-15%\uff0c\u5c3d\u7ba1\u53ef\u68c0\u6d4b\u7684\u4e0d\u540c\u7cd6\u578b\u53d8\u5316\u5728\u51e0\u79cd\u7ec6\u80de\u95f4\u662f\u5fae\u5999\u7684\uff0c\u4f46\u662f\u5176\u5bf9\u836f\u7269\u836f\u6548\u7684\u5f71\u54cd\u9700\u8981\u5177\u4f53\u95ee\u9898\u5177\u4f53\u5206\u6790\u3002<\/span><\/p>\n

\"\u4e0d\u540cCHO\u7ec6\u80de\u7cfb\u5728\u6297\u4f53\u8868\u8fbe\u4e0e\u751f\u7269\u91cf\u5408\u6210\u4e4b\u95f4\u7684\u6289\u62e9\"<\/p>\n

\u56fe<\/strong>4.<\/strong>\u4e09\u79cd\u7ec6\u80de\u7cfb\u5728\u4e24\u79cd\u57f9\u517b\u57fa\u91cc\u704c\u6ce8\u57f9\u517b\u6761\u4ef6\u4e0b\u6297\u4f53\u8f7b\u94fe\u7cd6\u578b\u5206\u5e03<\/strong><\/span><\/p>\n

\u54fa\u4e73\u52a8\u7269\u86cb\u767d\u7ffb\u8bd1\u540e\u4fee\u9970\u6709\u52a9\u4e8e\u86cb\u767d\u6b63\u786e\u6298\u53e0\uff0c\u7a33\u5b9a\uff0c\u4ee5\u53ca\u529f\u80fd\u53d1\u6325\u3002\u4e3a\u4e86\u63a2\u7a76\u4e0d\u540c\u7ec6\u80de\u7cfb\u6240\u4ea7\u540c\u79cd\u6297\u4f53\u7684\u6784\u50cf\u7a33\u5b9a\u6027\uff0c\u5bf9\u86cb\u767d\u8d28\u70ed\u7a33\u5b9a\u6027\u8fdb\u884c\u7814\u7a76\u3002DSC\u6d4b\u91cf\u53d1\u73b0\u56db\u79cd\u8fc7\u6e21\u6001\u86cb\u767d\u672a\u6298\u53e0\u5f62\u5f0f\uff0c\u975e\u5e38\u91cd\u8981\u7684\u662f\uff0c\u57f9\u517b\u57fa\u5bf9\u6297\u4f53\u7a33\u5b9a\u6027\u5e76\u6ca1\u6709\u5f71\u54cd\u3002CHO-S\u8868\u8fbe\u6297\u4f53\u89e3\u94fe\u6e29\u5ea6TM1\u4e3a64.3\u00b10.2\u2103\uff0c\u660e\u663e\u4f4e\u4e8eCHO-K1\u548cCHO-DG44\u8868\u8fbe\u6297\u4f53\u7684\u89e3\u94fe\u6e29\u5ea6TM166\u00b10.3\u2103 1.7\u2103\u3002\u8fdb\u4e00\u6b65\u5bf9\u8868\u8fbe\u6297\u4f53\u8d28\u8c31\u5206\u6790\u53d1\u73b0\uff0cCHO-S\u8868\u8fbe\u6297\u4f53\u7684\u8f7b\u94fe\u4e22\u5931\u4e86C\u672b\u7aef\u7684\u4e1d\u6c28\u9178Ser\u3002<\/span><\/p>\n

\"\u4e0d\u540cCHO\u7ec6\u80de\u7cfb\u5728\u6297\u4f53\u8868\u8fbe\u4e0e\u751f\u7269\u91cf\u5408\u6210\u4e4b\u95f4\u7684\u6289\u62e9\"<\/p>\n

\u56fe5.\u4e09\u79cd\u7ec6\u80de\u7cfb\u5728\u4e24\u79cd\u57f9\u517b\u57fa\u91cc\u704c\u6ce8\u57f9\u517b\u6761\u4ef6\u4e0b\u6297\u4f53\u70ed\u7a33\u5b9a\u6027\u5206\u6790<\/span><\/p>\n

\n
\n
3<\/section>\n
\u8ba8\u8bba<\/section>\n<\/section>\n<\/section>\n

\u65f6\u81f3\u4eca\u65e5\u51e0\u4e4e70%\u7684\u751f\u7269\u5236\u54c1\u90fd\u6765\u81ea\u4e8eCHO\u7ec6\u80de\u91cd\u7ec4\u8868\u8fbe\u3002\u5927\u91cf\u6587\u732e\u9c9c\u6709\u5bf9\u4e0d\u540c\u5bbf\u4e3b\u7ec6\u80de\u7cfb\u8fdb\u884c\u7c7b\u6bd4\u3002\u4e0d\u540c\u516c\u53f8\u6839\u636e\u81ea\u5df1\u7684\u9700\u6c42\u9009\u62e9\u7ec6\u80de\u7cfb\u6765\u751f\u4ea7\u81ea\u5df1\u6240\u9700\u8981\u7684\u4ea7\u54c1\uff0c\u8fd9\u4e00\u70b9\u65e0\u53ef\u539a\u975e\u3002\u4e3a\u4e86\u66f4\u597d\u5730\u9610\u8ff0\u9009\u62e9\u7684\u5408\u7406\u6027\uff0c\u6211\u4eec\u5bf9\u4e09\u79cd\u7ec6\u80de\u7cfb\u5728\u4e24\u79cd\u57f9\u517b\u57fa\uff0c\u4e09\u79cd\u57f9\u517b\u65b9\u5f0f\u4e0b\u6297\u4f53\u8868\u8fbe\u60c5\u51b5\u505a\u4e86\u7814\u7a76\uff0c\u7814\u7a76\u53d1\u73b0\uff0c\u5bbf\u4e3b\u7ec6\u80de\u7684\u751f\u7406\u6027\u504f\u597d\u6709\u5229\u4e8e\u751f\u7269\u8d28\u5408\u6210\u548c\u6297\u4f53\u7684\u8868\u8fbe\u3002<\/span><\/p>\n

3.1CHO<\/strong>\u5bbf\u4e3b\u7ec6\u80de\u5230\u5e95\u504f\u597d\u4e8e\u6297\u4f53\u751f\u4ea7\u8fd8\u662f\u751f\u7269\u91cf\u5408\u6210\uff1f<\/strong><\/span><\/p>\n

\u4e3a\u4e86\u5ba2\u89c2\u5730\u7c7b\u6bd4\u4e09\u79cd\u7ec6\u80de\u7cfb\u8868\u8fbe\u6297\u4f53\u7684\u60c5\u51b5\uff0c\u4e09\u79cd\u7ec6\u80de\u7cfb\u6700\u7ec8\u8f6c\u67d3\u8868\u8fbe\u540c\u4e00\u79cd\u6297\u4f53\u7684\u8d28\u7c92\uff0c\u6839\u636e\u751f\u4ea7\u5b9e\u8df5\u6311\u9009\u6700\u597d\u7684\u9002\u7528\u4e8e\u751f\u4ea7\u7684\u7ec6\u80de\u7cfb\u3002\u5728\u7ec6\u80de\u7cfb\u5236\u5907\u7b5b\u9009\u8fc7\u7a0b\u4e2d\uff0cCHO-K1\u90fd\u53d8\u73b0\u51fa\u4e86\u5b83\u4f18\u79c0\u7684\u6027\u80fd\u3002\u9002\u5e94\u6027\u57f9\u517b\u57fa\u63d0\u4f9b\u4e86\u7ec6\u80de\u589e\u6b96\uff0c\u86cb\u767d\u8868\u8fbe\uff0c\u9057\u4f20\u7269\u8d28\u7a33\u5b9a\u7b49\u4f17\u591a\u6761\u4ef6\uff0c\u4e09\u79cd\u7ec6\u80de\u7cfb\u5728\u9002\u5e94\u6027\u57f9\u517b\u57fa\u76f4\u63a5\u57f9\u517b\u6210\u529f\u83b7\u5f97\u4e86\u751f\u4ea7\u7528\u7ec6\u80de\u682a\u3002\u7ec6\u80de\u589e\u6b96\uff0c\u7ec6\u80de\u5bc6\u5ea6\u5728ActiCHOTM<\/sup>P\u57f9\u517b\u57fa\u4e2d\uff0c\u65e0\u8bba\u5206\u6279\uff0c\u8865\u6599\u5206\u6279\uff0c\u704c\u6d41\u57f9\u517b\u6761\u4ef6\u4e0b\u90fd\u663e\u8457\u9ad8\u4e8eCDCHO\u57f9\u517b\u57fa\u5728\u5404\u79cd\u57f9\u517b\u6761\u4ef6\u3002\u8fd9\u5c31\u8868\u660e\u57f9\u517b\u57fa\u4e2d\u8425\u517b\u7269\u8d28\u7684\u5e73\u8861\u5f71\u54cd\u7740\u7ec6\u80de\u682a\u7684\u6027\u80fd\u3002\u57f9\u517b\u57fa\u72ec\u7acb\u5b9e\u9a8c\u4e2d\u53d1\u73b0CHO-S\u663e\u793a\u51fa\u66f4\u9ad8\u7684\u589e\u6b96\u901f\u7387\uff0c\u9ad8\u4e8eCHO-K1,CHO-DG4410%-50%,\u8fd9\u5c5e\u4e8e\u7ec6\u80de\u682a\u56fa\u6709\u7684\u751f\u957f\u504f\u597d\u7279\u6027\uff0c\u5728\u672a\u8f6c\u67d3\u7684CHO-S\u5206\u6279\u57f9\u517b\u6768\u6761\u4ef6\u4e0b\u4e5f\u663e\u793a\u51fa\u6bd4CHO-K1,CHO-DG44\u589e\u6b96\u901f\u5ea6\u5feb\u7684\u7279\u70b9\u3002<\/span><\/p>\n

\u9ad8\u6297\u4f53\u6ef4\u5ea6\u548c\u76f8\u5bf9\u8f83\u4f4e\u7684\u751f\u7269\u91cf\u7d2f\u79ef\u9884\u793a\u7740CHO-K1\u6709\u6700\u597d\u7684\u6297\u4f53\u8868\u8fbe\u91cf\u3002\u800cCHO-S\u521a\u597d\u76f8\u53cd\uff0c\u6297\u4f53\u8868\u8fbe\u91cf\u6700\u4f4e\u3002CHO-DG44\u6297\u4f53\u8868\u8fbe\u91cf\u5904\u4e8eCHO-K1\u548cCHO-S\u4e2d\u95f4\u6c34\u5e73\u3002\u8fd9\u4e00\u8d8b\u52bf\u5728\u4e24\u79cd\u57f9\u517b\u57fa\uff0c\u4e09\u79cd\u57f9\u517b\u6761\u4ef6\u4e0b\u90fd\u662f\u4e00\u81f4\u7684\u3002\u5bf9\u6297\u4f53\u8f7b\u91cd\u94femRNA\u5b9a\u91cf\u5206\u6790\u53d1\u73b0\uff0c\u4e0d\u540c\u7684CHO\u7ec6\u80de\u7cfb\u91cc\uff0c\u5c3d\u7ba1\u4e24\u79cdmRNA\u6765\u81ea\u4e8e\u540c\u4e00\u4e2acDNABAC\uff0c\u4f46\u5176\u62f7\u8d1d\u6570\u6709\u5dee\u522b\uff0cLC\u53ea\u67091-2\u4e2a\u62f7\u8d1d\uff0cHC\u67093-4\u4e2a\u62f7\u8d1d\u3002\u5f53\u7136\uff0c\u8fd9\u5f88\u6709\u53ef\u80fd\u662f\u57fa\u7840\u5bf9\u6570\u8ba1\u7b97\u7684\u7ed3\u679c\u3002\u6211\u4eec\u7684\u6570\u636e\u663e\u793a\uff0c\u5728\u6574\u4e2a\u8fc7\u7a0b\u4e2d\uff0c\u4e0e\u4ea7\u54c1\u76f8\u5173\u7684mRNA\u4e0e\u7ec6\u80de\u7279\u5f02\u6027\u5355\u514b\u9686\u6297\u4f53\u7684\u751f\u4ea7\u7387\u4e4b\u95f4\u6ca1\u6709\u7edf\u8ba1\u5b66\u4e0a\u663e\u8457\u7684\u76f8\u5173\u6027\uff0c\u56e0\u4e3a\u8fd9\u4e24\u4e2a\u53c2\u6570\u90fd\u968f\u7740\u4e09\u4e2aCHO\u7ec6\u80de\u7cfb\u7684\u57f9\u517b\u65f6\u95f4\u800c\u53d8\u5316\u3002\u8003\u8651\u5230\u8fd9\u4e9b\u884d\u751f\u7684CHO\u7ec6\u80de\u7cfb\u7ecf\u5386\u8fc7\u5e7f\u6cdb\u7684\u57fa\u56e0\u7a81\u53d8\u548c\u514b\u9686\u9009\u62e9\uff0c\u6211\u4eec\u63a8\u6d4b\u51b3\u5b9a\u6297\u4f53\u8868\u8fbe\u5dee\u5f02\u7684\u5173\u952e\u4e0e\u7ec6\u80de\u8868\u578b\u548c\u4ee3\u8c22\u6709\u5173\uff0c\u4e0e\u7279\u5f02\u6027mRNA\u7684\u5dee\u522b\uff0c\u4ee5\u53ca\u57fa\u56e0\u63d2\u5165\u4f4d\u70b9\uff0c\u7ec6\u80de\u57f9\u517b\u57fa\u65e0\u5173\u3002\u57fa\u56e0\u5171\u8868\u8fbe\u7f51\u7edc\uff08GCN\uff09\u548c\u8f6c\u5f55\u91cf\u4e0e\u86cb\u767d\u8d28\u8868\u8fbe\u6c34\u5e73\u957f\u671f\u4ee5\u6765\u5df2\u6709\u591a\u6570\u6587\u7ae0\u62a5\u9053\uff0c\u4f46\u662f\u4ed6\u4eec\u4e4b\u95f4\u7684\u5173\u7cfb\u4ecd\u7136\u5b58\u5728\u7740\u4e89\u8bae\u3002<\/span><\/p>\n

CHO\u7ec6\u80de\u7cfb\u4ea7\u7269\u5408\u6210\u7387\u7684\u5dee\u5f02\u53ef\u80fd\u8981\u5f52\u548e\u4e8e\u4e0d\u540c\u7ec6\u80de\u7cfb\u4e0d\u7b49\u540c\u7684\u7ffb\u8bd1\u6548\u7387\u53ca\u5176\u5904\u7406\u52a0\u5de5\u7684\u5bb9\u91cf\u3002\u6bd4\u5982\u6700\u65b0\u53d1\u73b0\u7684CHO-K1\u7ec6\u80de\u6bd4\u8d77CHO-DUXB11\u7ec6\u80de\u6709\u66f4\u5927\u7684\u5185\u8d28\u7f51\uff0c\u66f4\u4e30\u5bcc\u7684\u7ebf\u7c92\u4f53\u4ee5\u53ca\u4e0e\u4e4b\u5bf9\u5e94\u7684\u9ad8\u7ec6\u80de\u7279\u5f02\u6027\u6297\u4f53\u8868\u8fbe\u91cf\u3002\u5185\u8d28\u7f51\u5bf9\u4e8e\u5206\u6ccc\u86cb\u767d\u7684\u6298\u53e0\u7ec4\u88c5\u975e\u5e38\u5173\u952e\uff0c\u5982\u679c\u4e00\u4e2a\u5185\u8d28\u7f51\u91cc\u4e0d\u53ef\u9006\u9519\u8bef\u6298\u53e0\u7684\u86cb\u767d\u8d85\u8fc7\u4e00\u5b9a\u9650\u5ea6\uff0c\u7ec6\u80de\u5c06\u542f\u52a8\u672a\u6298\u53e0\u86cb\u767d\u54cd\u5e94\u673a\u5236\uff0c\u6700\u7ec8\u53d1\u751f\u7ec6\u80de\u51cb\u4ea1\u3002\u56e0\u6b64\u5927\u5185\u8d28\u7f51\u6709\u5229\u4e8e\u5206\u6ccc\u86cb\u767d\u7684\u9ad8\u4ea7\u7387\u4e5f\u6709\u5229\u4e8e\u9ad8\u4ea7\u7ec6\u80de\u7684\u5b58\u6d3b\u548c\u86cb\u767d\u52a0\u5de5\u3002\u4e30\u5bcc\u7684\u7ebf\u7c92\u4f53\u57fa\u8d28\u6709\u5229\u4e8e\u7ec6\u80de\u81ea\u6211\u4f9b\u80fd\u4fbf\u4e8e\u5185\u8d28\u7f51\u52a0\u5de5\u5904\u7406\u672a\u6298\u53e0\u7684\u86cb\u767d\u3002\u7531\u4e8e\u5185\u8d28\u7f51\u548c\u7ebf\u7c92\u4f53\u5360\u636e\u4e86\u7ec6\u80de\u5185\u5927\u91cf\u7684\u7a7a\u95f4\uff0c\u56e0\u6b64\u6297\u4f53\u8868\u8fbe\u91cf\u4e5f\u53cd\u6620\u5728\u7ec6\u80de\u4f53\u79ef\u5927\u5c0f\u4e0a\uff0c\u6211\u4eec\u53d1\u73b0\u704c\u6d41\u57f9\u517b\u65f6\u9ad8\u4ea7\u7684CHO-K1\u7ec6\u80de\u76f4\u5f8415.7\u03bcm\u00b10.7 \uff0cCHO-DG44\u76f4\u5f8414.2\u03bcm\u00b10.5 \uff0cCHO-S\u76f4\u5f8413.7\u03bcm\u00b11.1\u3002<\/span><\/p>\n

3.2CHO<\/strong>\u5bbf\u4e3b\u7ec6\u80de\u4e0e\u6297\u4f53\u7cd6\u57fa\u5316\u4fee\u9970<\/strong><\/span><\/p>\n

\u7cd6\u57fa\u5316\u4fee\u9970\u5728\u5f88\u5927\u7a0b\u5ea6\u4e0a\u51b3\u5b9a\u4e86\u6297\u4f53\u7684\u6709\u6548\u6027\u548c\u5b89\u5168\u6027\u3002\u6539\u53d8Fc\u6bb5\u7cd6\u57fa\u5316\u4fee\u9970\u5c06\u5f71\u54cd\u5230Fc\u6bb5\u7684\u6784\u8c61\uff0c\u53d7\u4f53\u7ed3\u5408\uff0c\u4ee5\u53ca\u514d\u75ab\u529f\u80fd\u6548\u5e94\u3002<\/span><\/p>\n

\u6211\u4eec\u7684\u7814\u7a76\u663e\u793a\uff0c\u6297\u4f53\u7684\u7cd6\u57fa\u5316\u4e3b\u8981\u4e0e\u5bbf\u4e3b\u7ec6\u80de\u6709\u5173\uff0c\u8f83\u9ad8\u4e30\u5ea6\u7518\u9732\u7cd6\u7cd6\u57fa\u5316\uff0c\u8f83\u4f4e\u4e30\u5ea6\u5ca9\u85fb\u7cd6\u7cd6\u57fa\u5316\u6297\u4f53\u8868\u8fbe\u5bbf\u4e3b\u7ec6\u80de\u4f9d\u6b21\u4e3aCHO-DG44,CHO-K1,CHO-S\u3002\u9ad8\u7518\u9732\u7cd6\uff0c\u4f4e\u5ca9\u85fb\u7cd6\u6297\u4f53\u4e00\u822c\u6709\u8f83\u5f3a\u7684ADCC\u6548\u5e94\u3002\u534a\u4e73\u7cd6\u7cd6\u57fa\u5316\u53ef\u4ee5\u589e\u52a0\u6297\u4f53\u7684\u529f\u80fd\u6548\u5e94\uff0c\u534a\u4e73\u7cd6\u7cd6\u57fa\u5316\u4ec5\u53d7\u57f9\u517b\u57fa\u7684\u5f71\u54cd\u3002\u7136\u800c\u5728\u4f7f\u7528\u7684\u57f9\u517b\u57fa\u4e2d\u534a\u4e73\u7cd6\u5e76\u6ca1\u6709\u88ab\u91cf\u5316\uff0c\u4e00\u822c\u60c5\u51b5\u4e0bCHO-K1\u6709\u8f83\u9ad8\u7684\u534a\u4e73\u7cd6\u7cd6\u57fa\u5316\u6297\u4f53\u3002\u53ea\u6709\u6297\u4f53\u7684\u8f7b\u94fe\u7cd6\u57fa\u5316\u542b\u6709\u553e\u6db2\u9178\u6b8b\u57fa\uff0c\u5f88\u5927\u7a0b\u5ea6\u4e0a\u51b3\u5b9a\u4e86\u6297\u4f53\u7acb\u4f53\u6613\u63a5\u8fd1\u6027\u3002\u553e\u6db2\u9178\u5316\u4f5c\u7528\u4e5f\u6709\u52a9\u4e8e\u5ef6\u957f\u6297\u4f53\u7684\u534a\u8870\u671f\u548c\u6297\u708e\u75c7\u6548\u5e94\u3002\u553e\u6db2\u9178\u5316\u6297\u4f53\u5728CHO-K1\u8868\u8fbe\u7684\u6297\u4f53\u4e2d\u8f83\u9ad8\uff0cCHO-DG44,CHO-S\u6b21\u4e4b\u3002\u5bbf\u4e3b\u7ec6\u80de\u7279\u5f02\u6027\u7cd6\u57fa\u5316\u6a21\u5f0f\u53ef\u80fd\u4e0e\u5bbf\u4e3b\u57fa\u56e0\u5f02\u8d28\u6027\u5f02\u8d28\u6027\u6709\u5173\uff0c\u4e0d\u540c\u7684\u5bbf\u4e3b\u7ec6\u80de\u53ef\u80fd\u8868\u8fbe\u4e0d\u540c\u7684\u7cd6\u57fa\u5316\u57fa\u56e0\u3002Lewis<\/span>\u7b49\u4eba\u7684\u7814\u7a76\u53d1\u73b0\u4ed3\u9f20\u5c5e\u6709256\u79cd\u7cd6\u57fa\u5316\u9176\u76f8\u5173\u57fa\u56e0\uff0c13\u79cd\u975e\u540c\u4e49SNPs,2\u79cd\u63d2\u5165\u6216\u7f3a\u5931\u79fb\u7801\uff0c25%\u7684\u62f7\u8d1d\u6570\u53d8\u5f02CNVs,CHO-DG44,CHO-K1,CHO-S\u81f3\u5c11\u5b58\u5728\u4e00\u79cd\u4ee5\u4e0a\u60c5\u5f62\u3002\u867d\u7136\u4e0d\u662f\u6240\u6709\u7684SNPs\u5bf9\u4ea7\u7269\u7cd6\u57fa\u5316\u548c\u5bbf\u4e3b\u57fa\u8d28\u504f\u597d\u5f71\u54cd\u90fd\u80fd\u88ab\u68c0\u6d4b\u5230\uff0c\u4f46\u662f\u7cd6\u57fa\u5316\u662f\u4e2a\u6f2b\u957f\uff0c\u5206\u652f\u8fdb\u884c\u7684\u8fc7\u7a0b\uff0c\u8fd9\u5c31\u589e\u52a0\u4e86\u7531\u4e8e\u57fa\u56e0\u7a81\u53d8\u6539\u53d8\u6700\u7ec8\u7cd6\u57fa\u5316\u5f62\u5f0f\u7684\u53ef\u80fd\u3002\u6211\u4eec\u7684\u7ed3\u679c\u4e5f\u5e94\u8bc1\u4e86\u8fd9\u4e00\u5047\u8bbe\uff0c\u4e0d\u540c\u7684CHO\u8868\u8fbe\u7cfb\u7edf\u542b\u6709\u4e0d\u540c\u7684\u7cd6\u57fa\u5316\u6a21\u5f0f\uff0c\u8fd9\u6837\u5c31\u53ef\u4ee5\u6839\u636e\u9700\u6c42\u9009\u62e9\u4e0d\u540c\u7684\u5bbf\u4e3b\u751f\u4ea7\u9700\u8981\u7684\u4ea7\u7269\u3002<\/span><\/p>\n

\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u86cb\u767d\u751f\u4ea7\u8fc7\u7a0b\u4e2d\u7684\u7ffb\u8bd1\u540e\u4fee\u9970\u662f\u86cb\u767d\u8d28\u6b63\u786e\u6298\u53e0\uff0c\u7a33\u5b9a\uff0c\u53d1\u6325\u529f\u80fd\u7684\u5173\u952e\u3002\u86cb\u767d\u8d28\u70ed\u7a33\u5b9a\u6027\u6709\u52a9\u4e8e\u63d0\u9ad8\u86cb\u767d\u4ea7\u91cf\uff0c\u6211\u4eec\u7684\u7814\u7a76\u8868\u660eCHO-S\u8868\u8fbe\u86cb\u767d\u70ed\u7a33\u5b9a\u6027\u76f8\u5bf9\u6700\u5dee\uff0c\u86cb\u767d\u8868\u8fbe\u91cf\u4e5f\u6700\u4f4e\uff0c\u5c3d\u7ba1\u86cb\u767d\u8868\u8fbe\u91cf\u4e0aCHO-DG44\u6297\u4f53\u8868\u8fbe\u91cf\u6bd4CHO-S\u7ec6\u80de\u9ad8\u4e0d\u592a\u591a\uff0c\u4f46\u86cb\u767d\u7a33\u5b9a\u6027\u786e\u8ddfCHO-K1\u8868\u8fbe\u86cb\u767d\u63a5\u8fd1\u3002\u6297\u4f53CHO-S\u8868\u8fbe\u6297\u4f53\u7194\u89e3\u6e29\u5ea6Tm1\u76f8\u6bd4CHO-K1,CHO-DG44\u8868\u8fbe\u6297\u4f53\u4f4e\u51fa\u6765\u76841.7\u2103\u662f\u5426\u5f71\u54cd\u6297\u4f53\u8868\u8fbe\u91cf\u8fd8\u5f88\u96be\u786e\u8ba4\u3002\u6297\u4f53\u70ed\u7a33\u5b9a\u6027\u5dee\u53ef\u80fd\u7684\u539f\u56e0\u662fCHO-S\u8868\u8fbe\u6297\u4f53\u8f7b\u94feC\u7aefser\u7684\u7f3a\u5931\u3002\u8fd9\u4e00\u539f\u56e0\u662f\u5426\u662f\u76f4\u63a5\u5bfc\u81f4CHO\u7ec6\u80de\u7cfb\u8868\u8fbe\u6297\u4f53\u70ed\u7a33\u5b9a\u6027\u5dee\u8fd8\u4e0d\u5f97\u800c\u77e5\u3002Shen\u7b49\u4eba\u7684\u7814\u7a76\u8868\u660e\u5220\u9664\u03bb\u8f7b\u94feC\u7aefSer\u6709\u52a9\u4e8e\u63d0\u9ad8\u6297\u4f53\u7684\u70ed\u7a33\u5b9a\u6027\u548cPH\u4f9d\u8d56\u7684\u7a33\u5b9a\u6027\uff0c\u800c\u4e14\u5220\u9664Ser\u5e76\u6ca1\u6709\u5f71\u54cd\u6297\u4f53\u4e0e\u6297\u539f\u7684\u7ed3\u5408\u3002\u4f46\u5f53IgG1\u03bb\u8f7b\u94fe\u7684Ser\u5b58\u5728\u65f6\uff0c\u70ed\u7a33\u5b9a\u6027\u4f1a\u53d8\u5f97\u66f4\u9ad8\u3002\u57f9\u517b\u57fa\u5bf9\u6297\u4f53\u70ed\u7a33\u5b9a\u6027\u6ca1\u6709\u5f71\u54cd\u3002<\/span><\/p>\n

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\u7ed3\u8bba<\/section>\n<\/section>\n<\/section>\n

\u968f\u7740\u9ad8\u901a\u91cf\u6d4b\u5e8f\u7684\u53d1\u5c55\uff0c\u5927\u91cf\u6d4b\u5e8f\u6570\u636e\u663e\u793a\uff0c\u4e0d\u540cCHO\u7ec6\u80de\u7cfb\u5b58\u5728\u7740\u9057\u4f20\u7269\u8d28\u5f02\u8d28\u6027\u3002\u6709\u5173\u9057\u4f20\u7269\u8d28\u5dee\u5f02\u9020\u6210\u7684\u7ec6\u80de\u8868\u578b\u5dee\u5f02\u663e\u800c\u6613\u89c1\uff0c\u4f46\u662f\u5728\u751f\u7269\u5408\u6210\u6297\u4f53\u8868\u8fbe\u65b9\u9762\u5177\u4f53\u4f1a\u4ea7\u751f\u4ec0\u4e48\u5f71\u54cd\u62a5\u9053\u7684\u5e76\u4e0d\u591a\uff0c\u672c\u6587\u9610\u8ff0\u4e86\u7ec6\u80de\u8868\u578b\u51b3\u5b9a\u4e86CHO\u7ec6\u80de\u7279\u5f02\u6027\u504f\u597d\u8868\u8fbe\u6297\u4f53\u548c\u751f\u7269\u5408\u6210\u8fc7\u7a0b\u3002CHO-K1\u4ee3\u8c22\u504f\u597d\u4e8e\u6297\u4f53\u5f62\u6210\uff0cCHO-S\u4ee3\u8c22\u504f\u597d\u4e8e\u751f\u7269\u91cf\u5f62\u6210\uff0cCHO-DG44\u4ecb\u4e8e\u4e4b\u95f4\u3002\u8fd9\u79cd\u504f\u597d\u72ec\u7acb\u4e0e\u57f9\u517b\u6a21\u5f0f\u548c\u57f9\u517b\u57fa\u3002\u56e0\u6b64\u751f\u4ea7\u8fc7\u7a0b\u4e2d\u7ec6\u80de\u7cfb\u7684\u9009\u62e9\u5bf9\u4ea7\u7269\u6709\u6839\u672c\u6027\u7684\u5f71\u54cd\u3002\u5f53\u4e0bCHO\u7ec6\u80de\u7cfb\u57fa\u56e0\u7ec4\u548c\u8868\u578b\u5dee\u5f02\u8fd8\u4e0d\u592a\u6e05\u695a\uff0c\u76ee\u524d\u8fd8\u672a\u6210\u4e3a\u5bbf\u4e3b\u9009\u62e9\u8003\u8651\u7684\u5bf9\u8c61\u3002CHO\u5e8f\u5217\u7279\u5f02\u6027\u77e5\u8bc6\u4e0e\u7279\u5f02\u6027\u57f9\u517b\u65b9\u6cd5\u7684\u7ed3\u5408\uff0c\u5c06\u4e3a\u672a\u6765\u6784\u5efa\u7ec6\u80de\u7cfb\u5de5\u7a0b\u65b9\u6cd5\u548c\u7ec6\u80de\u7cfb\u540e\u7eed\u5de5\u827a\uff0c\u4ee5\u53ca\u751f\u7269\u8fc7\u7a0b\u548c\u7ec6\u80de\u57f9\u517b\u57fa\u7684\u6539\u8fdb\u63d0\u4f9b\u6709\u529b\u7684\u5de5\u5177\u3002\u672c\u7814\u7a76\u53d1\u73b0\u57fa\u56e0\u80cc\u666f\u5f71\u54cd\u7740\u5bbf\u4e3b\u7ec6\u80de\u7684\u8868\u578b\uff0c\u6700\u7ec8\u5f71\u54cd\u7740\u4ea7\u7269\u7684\u4ea7\u91cf\u548c\u7cd6\u57fa\u5316\u4fee\u9970\u7b49\u8d28\u91cf\u3002\u56e0\u6b64\u6b63\u786e\u7684\u9009\u62e9\u5bbf\u4e3b\u7ec6\u80de\u624d\u80fd\u8fbe\u5230\u751f\u4ea7\u6240\u9700\u6c42\u4ea7\u7269\u7684\u76ee\u7684\u3002<\/span><\/p>\n

\u539f\u6587\u6765\u6e90<\/span><\/strong>\uff1a<\/span>Reinhart D, Damjanovic L, Kaisermayer C,et,al. Bioprocessing of Recombinant CHO-K1, CHO-DG44, and CHO-S: CHO Expression Hosts FavorEither mAb Production or Biomass Synthesis.Biotechnol J. 2019 Mar;14(3):e1700686.<\/span><\/p>\n

\"\u4e0d\u540cCHO\u7ec6\u80de\u7cfb\u5728\u6297\u4f53\u8868\u8fbe\u4e0e\u751f\u7269\u91cf\u5408\u6210\u4e4b\u95f4\u7684\u6289\u62e9\"<\/span><\/span><\/a><\/p>\n

\u70b9\u51fb\u4e86\u89e3\u66f4\u591a\u8be6\u60c5\uff01<\/strong><\/span><\/p>\n


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\u8bc6\u522b\u5fae\u4fe1\u4e8c\u7ef4\u7801\uff0c\u6dfb\u52a0\u5c0f\u7f16\uff0c\u7b26\u5408\u6761\u4ef6\u8005\u5373\u53ef\u52a0\u5165\u751f\u7269\u5236\u54c1\u5fae\u4fe1\u7fa4\uff01<\/strong>
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\u8bf7\u6ce8\u660e\uff1a\u59d3\u540d+\u7814\u7a76\u65b9\u5411\uff01<\/strong><\/span><\/p>\n

\n
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\"\u4e0d\u540cCHO\u7ec6\u80de\u7cfb\u5728\u6297\u4f53\u8868\u8fbe\u4e0e\u751f\u7269\u91cf\u5408\u6210\u4e4b\u95f4\u7684\u6289\u62e9\"<\/p>\n

\"\u4e0d\u540cCHO\u7ec6\u80de\u7cfb\u5728\u6297\u4f53\u8868\u8fbe\u4e0e\u751f\u7269\u91cf\u5408\u6210\u4e4b\u95f4\u7684\u6289\u62e9\"
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\u7248\u6743\u4e3a\u6240\u6709\u3002<\/span>\u6b22\u8fce\u4e2a\u4eba\u8f6c\u53d1\u5206\u4eab\u3002<\/span>\u5176\u4ed6\u4efb\u4f55\u5a92\u4f53\u3001\u7f51\u7ad9\u5982\u9700\u8f6c\u8f7d\u6216\u5f15\u7528\u672c\u7f51\u7248\u6743\u6240\u6709\u5185\u5bb9<\/span>\u987b\u83b7\u5f97\u6388\u6743<\/strong>\u4e14\u5728\u9192\u76ee\u4f4d\u7f6e\u5904\u6ce8\u660e\u201c\u8f6c\u81ea\uff1a<\/span>\u201d\u3002<\/span>
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\"\u4e0d\u540cCHO\u7ec6\u80de\u7cfb\u5728\u6297\u4f53\u8868\u8fbe\u4e0e\u751f\u7269\u91cf\u5408\u6210\u4e4b\u95f4\u7684\u6289\u62e9\"<\/p>\n<\/section>\n<\/section><\/div>\n","protected":false},"excerpt":{"rendered":"

1 \u80cc\u666f\u4ecb\u7ecd \u4e2d\u56fd\u4ed3\u9f20\u5375\u5de2\u7ec6\u80deCHO\u5df2\u5f00\u53d1\u51fa\u4e86CHO-DXB11,CHO-K1, CHO-DG44, \u548cCHO […]<\/p>\n","protected":false},"author":1,"featured_media":107799,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/107798"}],"collection":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/comments?post=107798"}],"version-history":[{"count":1,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/107798\/revisions"}],"predecessor-version":[{"id":107811,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/107798\/revisions\/107811"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media\/107799"}],"wp:attachment":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media?parent=107798"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/categories?post=107798"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/tags?post=107798"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}