{"id":11186,"date":"2018-07-18T01:00:00","date_gmt":"2018-07-30T04:03:07","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/2018\/07\/30\/%e5%9b%bd%e5%ae%b6%e7%ba%b3%e7%b1%b3%e7%a7%91%e5%ad%a6%e4%b8%ad%e5%bf%83%e8%af%be%e9%a2%98%e7%bb%84crispr%e7%ba%b3%e7%b1%b3%e9%80%92%e9%80%81%e7%a0%94%e7%a9%b6%e5%8f%96%e5%be%97%e6%96%b0%e8%bf%9b\/"},"modified":"2018-07-30T12:03:08","modified_gmt":"2018-07-30T04:03:08","slug":"%e5%9b%bd%e5%ae%b6%e7%ba%b3%e7%b1%b3%e7%a7%91%e5%ad%a6%e4%b8%ad%e5%bf%83%e8%af%be%e9%a2%98%e7%bb%84crispr%e7%ba%b3%e7%b1%b3%e9%80%92%e9%80%81%e7%a0%94%e7%a9%b6%e5%8f%96%e5%be%97%e6%96%b0%e8%bf%9b","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/11186\/","title":{"rendered":"\u56fd\u5bb6\u7eb3\u7c73\u79d1\u5b66\u4e2d\u5fc3\u8bfe\u9898\u7ec4CRISPR\u7eb3\u7c73\u9012\u9001\u7814\u7a76\u53d6\u5f97\u65b0\u8fdb\u5c55"},"content":{"rendered":"<div class=\"bpp-post-content\">\n<p>\u8fd1\u65e5\uff0c\u56fd\u5bb6\u7eb3\u7c73\u79d1\u5b66\u4e2d\u5fc3\u848b\u5174\u5b87\u7814\u7a76\u5458\u3001\u90d1\u6587\u5bcc\u7814\u7a76\u5458\u5e26\u9886\u7684\u8bfe\u9898\u7ec4\u53d1\u8868\u4e86\u975e\u75c5\u6bd2\u7eb3\u7c73\u8f7d\u4f53\u9012\u9001\u7684\u7814\u7a76\u6210\u679c\u3002\u4ed6\u4eec\u5f00\u53d1\u4e86\u4e00\u7cfb\u5217\u975e\u75c5\u6bd2\u7684\u7eb3\u7c73\u8f7d\u4f53\uff0c\u8fd9\u4e9b\u975e\u75c5\u6bd2\u7eb3\u7c73\u8f7d\u4f53\u53ef\u4ee5\u9ad8\u6548\u9012\u9001CRISPR\/Cas9\u7cfb\u7edf\u5230\u4f53\u5185\uff0c\u4e3a\u62d3\u5c55\u8fd9\u4e00\u5f3a\u5927\u57fa\u56e0\u7f16\u8f91\u6280\u672f\u5728\u751f\u547d\u79d1\u5b66\u53ca\u548c\u4e34\u5e8a\u5e94\u7528\u9886\u57df\u7684\u5e94\u7528\u63d0\u4f9b\u4e86\u65b0\u9014\u5f84\u3002\u76f8\u5173\u7814\u7a76\u6210\u679c\u201cThermo-triggered release of CRISPR-Cas9 system by lipid-encapsulated gold nanoparticles for tumor therapy\u201d&nbsp;\u4f5c\u4e3aHot paper\u5728Angew Chem Int Ed&nbsp;(57, 1491, 2018)\u53d1\u8868\u3002<\/p>\n<p><br  \/><\/p>\n<p><img class=\"\"     width=\"500\" style=\"margin-right: auto;margin-left: auto;height: 225px;white-space: normal;box-sizing: border-box;border-width: 0px;border-style: initial;border-color: initial;vertical-align: middle;display: block;width: 238px;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/07\/beepress3-1532923387.jpg\" alt=\"\u56fd\u5bb6\u7eb3\u7c73\u79d1\u5b66\u4e2d\u5fc3\u8bfe\u9898\u7ec4CRISPR\u7eb3\u7c73\u9012\u9001\u7814\u7a76\u53d6\u5f97\u65b0\u8fdb\u5c55\" title=\"\u56fd\u5bb6\u7eb3\u7c73\u79d1\u5b66\u4e2d\u5fc3\u8bfe\u9898\u7ec4CRISPR\u7eb3\u7c73\u9012\u9001\u7814\u7a76\u53d6\u5f97\u65b0\u8fdb\u5c55\"  \/><\/p>\n<p>\u3000\u3000CRISPR\/Cas9\u7cfb\u7edf\u4f5c\u4e3a\u57fa\u56e0\u7f16\u8f91\u6280\u672f\u7684\u5f04\u6f6e\u513f\uff0c\u5177\u6709\u5de8\u5927\u7684\u6f5c\u5728\u5e94\u7528\u3002\u4f46\u662f\u76ee\u524d\u5927\u90e8\u5206\u65b9\u6cd5\u90fd\u662f\u5229\u7528\u75c5\u6bd2\u8f7d\u4f53\u5bfc\u5165\u5230\u751f\u547d\u4f53\uff0c\u6240\u4ee5\u6781\u5927\u5730\u9650\u5236\u4e86\u5176\u5728\u4e34\u5e8a\u7684\u5e94\u7528\u524d\u666f\u3002\u7136\u800c\uff0c\u75c5\u6bd2\u8f7d\u4f53\u5bf9\u5bbf\u4e3b\u7ec6\u80de\u53ef\u80fd\u4ea7\u751f\u81f4\u764c\u3001\u81f4\u7a81\u53d8\u7684\u98ce\u9669\uff0c\u56e0\u6b64\u4e0d\u80fd\u5b9e\u73b0\u5bf9CRISPR\/Cas9\u7cfb\u7edf\u7684\u9ad8\u6548\u800c\u5b89\u5168\u7684\u9012\u9001\u5df2\u7ecf\u6210\u4e3a\u963b\u788d\u8be5\u6280\u672f\u4e34\u5e8a\u5e94\u7528\u7684\u4e3b\u8981\u74f6\u9888\u3002\u751f\u7269\u6750\u6599\u9886\u57df\u7684\u79d1\u5b66\u5bb6\u5c1d\u8bd5\u7740\u5229\u7528\u4eba\u5de5\u8f7d\u4f53\uff0c\u4f8b\u5982\u8102\u8d28\u4f53\u3001\u7eb3\u7c73\u6750\u6599\u7b49\u628a\u7f16\u7801\u7684CRISPR\/Cas9\u7684\u8d28\u7c92\u5bfc\u5165\u7ec6\u80de\u3002<\/p>\n<p><br  \/><\/p>\n<p>\u848b\u5174\u5b87\u8bfe\u9898\u7ec4\u53d1\u5c55\u4e86\u57fa\u4e8e\u91d1\u7eb3\u7c73\u9897\u7c92-\u8102\u8d28\u4f53\u4f53\u7cfb\u7684\u5149\u63a7\u91ca\u653e\u7eb3\u7c73\u9012\u9001\u7cfb\u7edf\u3002\u4ed6\u4eec\u5c06\u91d1\u7eb3\u7c73\u9897\u7c92\u8868\u9762\u4fee\u9970TAT\u591a\u80bd\uff0c\u4f7f\u7eb3\u7c73\u9897\u7c92\u8868\u9762\u5e26\u6b63\u7535\u8377\uff0c\u80fd\u591f\u548c\u5e26\u8d1f\u7535\u8377\u7684\u8868\u8fbeCas9\u86cb\u767d\u548c\u5f15\u5bfcRNA\u7684\u8d28\u7c92\uff08Cas9\/sgRNA plasmid\uff09\u7ed3\u5408\uff0c\u5f62\u6210\u4e00\u4e2a\u6574\u4f53\u4e0a\u5e26\u8d1f\u7535\u8377\u7684\u201c\u7eb3\u7c73\u6838\u201d\uff0c\u518d\u5728\u8be5\u201c\u6838\u201d\u5916\u5305\u88f9\u5e26\u6b63\u7535\u8377\u7684\u8102\u8d28\u4f53\u5c42\uff08DOTAP, DOPE, Cholesterol\uff09\u4ee5\u53caPEG2000-DSPE\uff0c\u5f62\u6210\u4e00\u4e2a\u5177\u6709\u6838\u58f3\u7ed3\u6784\u7684\u7eb3\u7c73\u9897\u7c92\u3002\u8be5\u7eb3\u7c73\u9897\u7c92\u53ef\u4ee5\u901a\u8fc7\u7ec6\u80de\u7684\u80de\u541e\u53ca\u6eb6\u9176\u4f53\u9003\u9038\u9014\u5f84\u8fdb\u5165\u7ec6\u80de\u6d46\uff0c\u5728514\u7eb3\u7c73\u6fc0\u5149\u7167\u5c04\u4e0b\u91d1\u9897\u7c92\u548cTAT\u4e4b\u95f4\u7684\u91d1-\u786b\u952e\u88ab\u6253\u5f00\u4ece\u800c\u5c06\u4fee\u9970\u5728\u91d1\u9897\u7c92\u4e0a\u7684TAT\u591a\u80bd\u89e3\u79bb\u4e0b\u6765\uff0c\u4e0eTAT\u591a\u80bd\u901a\u8fc7\u9759\u7535\u76f8\u4e92\u4f5c\u7528\u7ed3\u5408\u7684Cas9\/sgRNA plasmid\u4e5f\u968f\u4e4b\u89e3\u79bb\u4e0b\u6765\u5e76\u5728TAT\u591a\u80bd\u7684\u6307\u5f15\u4e0b\u7a7f\u8fc7\u7ec6\u80de\u6838\u819c\u8fdb\u5165\u7ec6\u80de\u6838\u3002<\/p>\n<p><br  \/><\/p>\n<p>\u5229\u7528\u8be5\u7eb3\u7c73\u8f7d\u4f53\uff0c\u7814\u7a76\u7ec4\u5728\u4f53\u5916\u4f53\u5185\u5b9e\u73b0\u4e86\u5bf9\u80bf\u7624\u764c\u57fa\u56e0polo-like-kinase-1(Plk-1)\u7684\u9776\u5411\u6572\u9664\u5e76\u6709\u6548\u63a7\u5236\u4e86\u80bf\u7624\u7684\u751f\u957f\u548c\u8f6c\u79fb\u3002<\/p>\n<p><img class=\"\"     width=\"500\" style=\"margin-right: auto;margin-left: auto;height: 222px;white-space: normal;box-sizing: border-box;border-width: 0px;border-style: initial;border-color: initial;vertical-align: middle;display: block;width: 250px;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/07\/beepress0-1532923388.jpg\" alt=\"\u56fd\u5bb6\u7eb3\u7c73\u79d1\u5b66\u4e2d\u5fc3\u8bfe\u9898\u7ec4CRISPR\u7eb3\u7c73\u9012\u9001\u7814\u7a76\u53d6\u5f97\u65b0\u8fdb\u5c55\" title=\"\u56fd\u5bb6\u7eb3\u7c73\u79d1\u5b66\u4e2d\u5fc3\u8bfe\u9898\u7ec4CRISPR\u7eb3\u7c73\u9012\u9001\u7814\u7a76\u53d6\u5f97\u65b0\u8fdb\u5c55\"  \/><\/p>\n<p>\u8be5\u5de5\u4f5c\u662f\u5728\u524d\u671f\u5de5\u4f5c\u7684\u57fa\u7840\u4e0a\u53d1\u5c55\u800c\u6765\u7684\u3002\u5728\u7a0d\u65e9\u7684\u4e00\u4e9b\u5de5\u4f5c\u4e2d\uff0c\u848b\u5174\u5b87\u8bfe\u9898\u7ec4\u6210\u529f\u5229\u7528\u5fae\u6d41\u63a7\u7cfb\u7edf\u9ad8\u901a\u91cf\u7b5b\u9009\u4e8654\u79cd\u7eb3\u7c73\u9012\u9001\u7cfb\u7edf\u5e76\u6700\u7ec8\u4f18\u9009\u4e86\u8102\u8d28\u4f53\u7cfb\u7edf\u6210\u529f\u9012\u9001\u4e86Cas9\/sgRNA plasmid\u5230\u52a8\u7269\u4f53\u5185\uff0c\u5b9e\u73b0\u4e86\u5bf9\u80bf\u7624Plk-1\u57fa\u56e0\u7684\u9ad8\u6548\u6572\u9664(NPG Asia Mater, 9, e441, 2017)\uff1b\u5728\u6b64\u57fa\u7840\u4e0a\uff0c\u4ed6\u4eec\u53c8\u53d1\u5c55\u4e86\u57fa\u4e8e\u91d1\u7eb3\u7c73\u7c07-\u8102\u8d28\u4f53\u7684\u9012\u9001\u7cfb\u7edf\u5e76\u6210\u529f\u9012\u9001Cas9\u86cb\u767d\u548csgRNA plasmid\u9776\u5411\u52a8\u7269\u7684Plk-1\u57fa\u56e0\uff0c\u5b9e\u73b0\u4e86\u5bf9\u80bf\u7624\u7684\u6709\u6548\u6291\u5236(Adv Sci, 4, 1700175, 2017)\u3002<\/p>\n<p><br  \/><\/p>\n<p>\u848b\u5174\u5b87\u8bfe\u9898\u7ec4\u7684\u7cfb\u5217\u7814\u7a76\u5de5\u4f5c\u5f97\u5230\u4e86\u56fd\u5bb6\u81ea\u7136\u79d1\u5b66\u57fa\u91d1\u59d4\u3001\u4e2d\u79d1\u9662\u7eb3\u7c73\u5148\u5bfc\u4e13\u9879\u4ee5\u53ca\u4e2d\u79d1\u9662\u201c\u521b\u65b0\u56e2\u961f\u56fd\u9645\u5408\u4f5c\u4f19\u4f34\u8ba1\u5212\u201d\u7b49\u9879\u76ee\u7684\u652f\u6301\u3002<\/p>\n<p><br  \/><\/p>\n<p><span style=\"font-size: 14px;\">\u8bba\u6587\u94fe\u63a5\uff1ahttps:\/\/onlinelibrary.wiley.com\/doi\/abs\/10.1002\/anie.201708689<\/span><\/p>\n<p><br  \/><\/p>\n<p><br  \/><\/p>\n<p><br  \/><\/p>\n<blockquote class='keep-source'>\n<p>\u59cb\u53d1\u4e8e\u5fae\u4fe1\u516c\u4f17\u53f7\uff1a                         \u7cfb\u7edf                      <\/p>\n<\/blockquote>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u8fd1\u65e5\uff0c\u56fd\u5bb6\u7eb3\u7c73\u79d1\u5b66\u4e2d\u5fc3\u848b\u5174\u5b87\u7814\u7a76\u5458\u3001\u90d1\u6587\u5bcc\u7814\u7a76\u5458\u5e26\u9886\u7684\u8bfe\u9898\u7ec4\u53d1\u8868\u4e86\u975e\u75c5\u6bd2\u7eb3\u7c73\u8f7d\u4f53\u9012\u9001\u7684\u7814\u7a76\u6210\u679c\u3002\u4ed6\u4eec\u5f00\u53d1\u4e86\u4e00\u7cfb [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":11187,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[2],"tags":[],"_links":{"self":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/11186"}],"collection":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/comments?post=11186"}],"version-history":[{"count":1,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/11186\/revisions"}],"predecessor-version":[{"id":11190,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/11186\/revisions\/11190"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media\/11187"}],"wp:attachment":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media?parent=11186"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/categories?post=11186"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/tags?post=11186"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}