{"id":126497,"date":"2019-10-15T03:55:50","date_gmt":"2020-04-21T02:17:20","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/126497\/"},"modified":"2020-04-21T10:17:22","modified_gmt":"2020-04-21T02:17:22","slug":"9b84e90f31","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/126497\/","title":{"rendered":"\u200b\u6253\u8138\uff0c\u641e\u79d1\u7814\u7684\u6211\uff0c\u6bd4\u7237\u7237\u5976\u5976\u8fd8\u8ff7\u4fe1 | \u79d1\u7814\u597d\u7269\u8bd5\u7528\u4e0a\u65b0"},"content":{"rendered":"
\n
<\/section>\n
<\/section>\n

\u5bf9\u4e8e\u5e7f\u5927\u79d1\u7814\u72d7\u800c\u8a00\uff0c\u5b9e\u9a8c\u7ed3\u679c\u7edd\u5bf9\u662f\u96be\u4ee5\u638c\u63a7\u7684\u4e8b\u60c5\u3002\u6240\u4ee5\uff0c\u4e3a\u4e86\u79d1\u7814\uff0c\u5404\u79cd\u7948\u6c42\u795e\u79d8\u529b\u91cf\u7684\u4f20\u8bf4\u5728\u5404\u4e2a\u5b9e\u9a8c\u5ba4\u4e4b\u95f4\u6d41\u4f20\uff0c\u4ece\u672a\u95f4\u65ad\u2026\u2026<\/span><\/p>\n


<\/p>\n

\n
\n
\n
\n
\n
\n
\"\u200b\u6253\u8138\uff0c\u641e\u79d1\u7814\u7684\u6211\uff0c\u6bd4\u7237\u7237\u5976\u5976\u8fd8\u8ff7\u4fe1<\/section>\n
\n

\u795e\u79d8\u4eba 1 \u53f7<\/strong><\/span><\/strong><\/span><\/p>\n


<\/p>\n<\/section>\n<\/section>\n

\n
<\/section>\n
<\/section>\n
\n
<\/span>\u542c\u8bf4\u4e00\u54e5\u4eec\u513f\uff0c\u76f8\u4fe1\u4e07\u7269\u7686\u6709\u7075\uff0c\u4e07\u7269\u81ea\u7136\u4e5f\u5305\u62ec\u8001\u9f20\u5566\u3002<\/span>\u6240\u4ee5\uff0c\u4ed6\u6709\u65f6\u5019\u4f1a\u795e\u795e\u53e8\u53e8\u5730\u8ddf\u8001\u9f20\u5520\u55d1\uff0c\u8ba9\u5b83\u4eec\u5e2e\u5e2e\u5fd9\u5565\u7684\uff1b<\/span>\u8fd8\u7ecf\u5e38\u665a\u4e0a\u8dd1\u5230\u52a8\u7269\u623f\uff0c\u53ee\u5631\u5404\u4f4d\u8001\u9f20\u5927\u54e5\u5927\u59d0\u4eec\u597d\u597d\u4f11\u606f\uff0c\u8ddf\u4ed6\u4eec\u9053\u4e00\u58f0\u665a\u5b89\u3002<\/span>\u5230\u4e86\u5904\u6b7b\u7684\u8001\u9f20\u7684\u65f6\u5019\uff0c\u66f4\u662f\u8981\u7948\u7977\u7948\u7977\u518d\u7948\u7977\u3002<\/span><\/span><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

<\/span><\/p>\n

\n
\n


<\/p>\n

\n
\n
\n
\n
\"\u200b\u6253\u8138\uff0c\u641e\u79d1\u7814\u7684\u6211\uff0c\u6bd4\u7237\u7237\u5976\u5976\u8fd8\u8ff7\u4fe1<\/section>\n
\n

\u795e\u79d8\u4eba 2 \u53f7<\/strong><\/span><\/strong><\/span><\/p>\n


<\/p>\n<\/section>\n<\/section>\n

\n
<\/section>\n
<\/section>\n
\n

<\/span><\/p>\n

\u5b9e\u9a8c\u5ba4\u6709\u4e24\u53f0 PCR \u4eea\uff0c\u4e00\u53f0\u662f\u5341\u51e0\u5e74\u524d\u4e70\u7684\uff0c\u4e00\u53f0\u662f\u8fd1\u4e24\u5e74\u624d\u4e70\u7684\u89e6\u5c4f\uff0c\u5927\u5bb6\u90fd\u62a2\u7740\u7528\u65b0\u7684\u3002\u53ef\u5e08\u5144\u5219\u76f8\u53cd\uff0c\u53ea\u7528\u65e7 \u7684\u3002<\/span>
<\/section>\n
 <\/span><\/section>\n
\u600e\u4e48\u56de\u4e8b\u5462\uff1f\u539f\u6765\u4ed6\u4e4b\u524d\u7528\u65b0 PCR \u4eea\u4e00\u76f4\u6ca1\u6709\u6269\u589e\u51fa\u76ee\u7684 DNA \u7247\u6bb5\u3002\u67d0\u5929\u51c6\u5907\u91cd\u65b0\u505a PCR \u7684\u65f6\u5019\uff0c\u65b0\u4eea\u5668\u5df2\u7ecf\u88ab\u4eba\u7528\u4e86\uff0c\u4e0d\u5f97\u5df2\u53ea\u597d\u7528\u65e7\u7684 PCR \u4eea\u3002\u7ed3\u679c\u7adf\u7136<\/span>\u6210\u529f\u4e86\u3002<\/span><\/section>\n
 <\/span><\/section>\n
\u4e8e\u662f\uff0c\u6267\u62d7\u7684\u5e08\u5144\u8ba4\u5b9a\u81ea\u5df1\u8ddf\u65b0\u4eea\u5668\u516b\u5b57\u4e0d\u5408\uff0c\u4ece\u6b64\u53ea\u4e13\u60c5\u4e8e\u65e7 PCR \u4eea\u3002<\/span><\/section>\n<\/section>\n<\/section>\n<\/section>\n
<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n


<\/p>\n

\u7c7b\u4f3c\u7684\u4f8b\u5b50\u8fd8\u6709\u5f88\u591a\u3002\u6709\u7684\u4eba\u300c\u949f\u60c5\u300d\u4e8e\u7279\u5b9a\u7684\u4e00\u628a\u79fb\u6db2\u67aa\uff0c\u78b0\u5230\u91cd\u8981\u7684\u5b9e\u9a8c\u5c31\u50cf\u8bf7\u51fa\u5c1a\u65b9\u5b9d\u5251\u4e00\u6837\u8bf7\u51fa\u5b83\uff0c\u6216\u8005\u662f\u7279\u5b9a\u7684\u65f6\u95f4\u6bb5\uff08\u636e\u8bf4\u534a\u591c\u662f\u5f88\u591a\u79d1\u7814\u5927\u795e\u7684\u6700\u7231\uff09\uff0c\u7279\u5b9a\u7684\u5b9e\u9a8c\u64cd\u4f5c\u53f0\u3002<\/span><\/p>\n

 <\/span><\/p>\n

\u8fd9\u4e9b\u4e00\u770b\u5c31\u300c\u4e0d\u79d1\u5b66\u300d\u7684\u64cd\u4f5c\uff0c\u4e5f\u8bb8\u4e0d\u4f1a\u771f\u7684\u8ba9\u6211\u4eec\u7684\u79d1\u7814\u4e4b\u8def\u4e00\u5e06\u98ce\u987a\uff0c\u4f46\u662f\u5982\u679c\u80fd\u591f\u6216\u591a\u6216\u5c11\u5730\u7f13\u89e3\u5927\u5bb6\u7684\u538b\u529b\u548c\u7126\u8651\uff0c\u4e5f\u672a\u5fc5\u5c31\u4e0d\u662f\u4e00\u4ef6\u597d\u4e8b\u3002<\/strong><\/span><\/p>\n


<\/strong><\/span><\/p>\n

\u4e00\u4e2a\u771f\u8bda\u8138\uff1a<\/span><\/section>\n
\u540c\u5b66\uff0c<\/span><\/section>\n
\u5b9e\u9a8c\u505a\u7684\u600e\u4e48\u6837\u4e86\uff1f<\/span><\/section>\n
\u7ecf\u8d39\u4e0d\u591f\uff1f<\/span><\/section>\n
\u8d28\u7591\u4ea7\u54c1\uff1f<\/span><\/section>\n
\"\u200b\u6253\u8138\uff0c\u641e\u79d1\u7814\u7684\u6211\uff0c\u6bd4\u7237\u7237\u5976\u5976\u8fd8\u8ff7\u4fe1
<\/section>\n

<\/section>\n
\u4e0d\u7528\u6015\uff0c<\/span>0 \u5143\u8bd5\u7528<\/strong><\/span>\u89e3\u51b3\u4f60\u7684\u95ee\u9898\uff0c<\/span>70 \u591a\u6b3e\u5b9e\u9a8c\u597d\u7269\u5148\u8bd5\u7528~<\/strong><\/span>\"\u200b\u6253\u8138\uff0c\u641e\u79d1\u7814\u7684\u6211\uff0c\u6bd4\u7237\u7237\u5976\u5976\u8fd8\u8ff7\u4fe1<\/strong><\/span><\/section>\n

<\/strong><\/span><\/section>\n
<\/section>\n
\"\u200b\u6253\u8138\uff0c\u641e\u79d1\u7814\u7684\u6211\uff0c\u6bd4\u7237\u7237\u5976\u5976\u8fd8\u8ff7\u4fe1<\/section>\n
<\/section>\n
<\/span>\u56fe\u7247\u6765\u6e90\uff1a\u4e01\u9999\u901a<\/span><\/section>\n

<\/span><\/section>\n
<\/section>\n
\u5148\u6765\u770b\u770b\u4e00\u4e9b\u8bd5\u7528\u597d\u7269\uff0c\u5168\u90e8\u8bd5\u7528\u9700\u8bc6\u522b\u4e0a\u56fe\u4e8c\u7ef4\u7801<\/strong>\u8fdb\u5165\u4f1a\u573a\u67e5\u770b~<\/span><\/section>\n

<\/section>\n
\"\u200b\u6253\u8138\uff0c\u641e\u79d1\u7814\u7684\u6211\uff0c\u6bd4\u7237\u7237\u5976\u5976\u8fd8\u8ff7\u4fe1SDS-PAGE <\/span><\/strong>\u51dd\u80f6\u5feb\u901f\u5236\u5907\u8bd5\u5242\u76d2<\/span><\/strong><\/span><\/section>\n
<\/section>\n
\u63d0\u4f9b\u4e86\u914d\u5236 SDS-PAGE \u51dd\u80f6\u6240\u9700\u7684\u5404\u79cd\u8bd5\u5242\uff0c\u7528\u6237\u53ea\u9700\u81ea\u5907\u5236\u80f6\u5668\u5177\u548c\u53cc\u84b8\u6c34\uff0c\u7b80\u5316\u4e86\u51dd\u80f6\u914d\u5236\u7684\u6b65\u9aa4\u3002<\/span><\/section>\n

<\/span><\/section>\n
\"\u200b\u6253\u8138\uff0c\u641e\u79d1\u7814\u7684\u6211\uff0c\u6bd4\u7237\u7237\u5976\u5976\u8fd8\u8ff7\u4fe1-Freezing-Medium <\/span><\/strong><\/span>\u65e0\u8840\u6e05\u51bb\u5b58\u6db2<\/span><\/strong><\/section>\n
\u5316\u5b66\u6210\u5206\u660e\u786e\uff0c\u65e0\u4efb\u4f55\u5916\u6e90\u86cb\u767d\u548c\u8840\u6e05\uff0c\u9002\u7528\u4e8e\u5404\u7c7b\u52a8\u7269\u7ec6\u80de\u682a\u51bb\u5b58\u3002\u65e0\u9700\u7a0b\u5e8f\u964d\u6e29\uff0c\u7ec6\u80de\u590d\u82cf\u7387 90% \u4ee5\u4e0a\u3002\u51bb\u5b58\u7ec6\u80de\u53ef\u76f4\u63a5\u5b58\u653e\u4e8e -80\u2103 \u51b0\u7bb1\uff0c\u7a33\u5b9a\u4fdd\u5b58\u6570\u5e74\u3002<\/span><\/section>\n

<\/section>\n
\"\u200b\u6253\u8138\uff0c\u641e\u79d1\u7814\u7684\u6211\uff0c\u6bd4\u7237\u7237\u5976\u5976\u8fd8\u8ff7\u4fe1Gibco \u6fb3\u6d32\u80ce\u725b\u8840\u6e05<\/span><\/strong><\/span><\/span><\/section>\n
\u5177\u6709\u6781\u4f4e\u7684 BSE \u548c\u75c5\u6bd2\u611f\u67d3\u98ce\u9669\uff0c\u7b26\u5408 USP \/ EP \u6807\u51c6\uff0c\u5e76\u901a\u8fc7\u76f8\u5e94\u7684\u591a\u8fbe 90 \u9879\u8d28\u91cf\u6d4b\u8bd5\u3002\u5927\u591a\u6570\u4ea7\u54c1\u7ecf\u8fc7\u4e09\u7ea7 0.1\u03bcm \u8fc7\u6ee4\u5236\u9020\u3002<\/span><\/section>\n

<\/section>\n
\"\u200b\u6253\u8138\uff0c\u641e\u79d1\u7814\u7684\u6211\uff0c\u6bd4\u7237\u7237\u5976\u5976\u8fd8\u8ff7\u4fe1\u6cf0\u56fd\u8fdb\u53e3\u65bd\u777f\u5eb7\u533b\u7597\u7ea7<\/span><\/strong><\/span>\u4e00\u6b21\u6027\u65e0\u7c89\u4e73\u80f6\u624b\u5957<\/span><\/strong><\/section>\n
\u539f\u6750\u6599\u9009\u7528\u6cf0\u56fd\u4f18\u8d28\u5929\u7136\u6a61\u80f6\uff0c\u6781\u4f73\u7684\u575a\u97e7\u5ea6\u4e0e\u9ad8\u5f39\u529b\uff0c\u201c\u5168\u9ebb\u201d\u80fd\u66f4\u597d\u7684\u6293\u63e1\uff0c\u4e25\u683c\u6309\u7167ISO\/GB\u8d28\u91cf\u6807\u51c6\u4f53\u7cfb\u751f\u4ea7\uff0c\u4ea7\u54c1\u901a\u8fc7\u7f8e\u56fdFDA\u3001\u4e2d\u56fdCFDA\u8ba4\u8bc1\u3002<\/span><\/section>\n

<\/section>\n
\"\u200b\u6253\u8138\uff0c\u641e\u79d1\u7814\u7684\u6211\uff0c\u6bd4\u7237\u7237\u5976\u5976\u8fd8\u8ff7\u4fe1\u9ad8\u6548\u7387\u6269\u589e PCR \u9884\u6df7\u6db2 <\/span><\/strong><\/span>Plus Mix<\/span><\/strong><\/section>\n
\u4f7f\u7528\u65f6\uff0c\u4ec5\u9700\u5728\u6269\u589e\u4f53\u7cfb\u4e2d\u52a0\u5165\u6a21\u677f\u548c\u5f15\u7269\u5373\u53ef\uff0c\u5927\u5927\u7b80\u5316\u64cd\u4f5c\u8fc7\u7a0b\uff0c\u7f29\u77ed\u64cd\u4f5c\u65f6\u95f4\uff0c\u964d\u4f4e\u6c61\u67d3\uff08\u52a0\u6837\u6b21\u6570\u51cf\u5c11\uff09\u3002\u542b\u6709\u4f18\u5316\u5242\u4e0e\u589e\u5f3a\u5242\uff0c\u80fd\u591f\u663e\u8457\u589e\u5f3aPCR\u6269\u589e\u7684\u7075\u654f\u5ea6\u3002<\/span><\/section>\n
<\/section>\n
\n
\n
\n
\n
\n
<\/section>\n
<\/section>\n
<\/section>\n
<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

<\/section>\n
\u66f4\u591a\u8bd5\u7528\u4ea7\u54c1\u70b9\u51fb\u9605\u8bfb\u5168\u6587<\/strong>\u8fdb\u5165\u4f1a\u573a\u67e5\u770b~<\/span>
<\/section>\n
<\/section>\n

\u5c01\u9762\u56fe\u6765\u6e90\uff1a\u7ad9\u9177\u6d77\u6d1b<\/span><\/p>\n

\u70b9\u51fb\u9605\u8bfb\u539f\u6587\u8fdb\u5165\u4f1a\u573a<\/span><\/p>\n

\u25bc\u25bc\u25bc<\/span><\/p>\n<\/p><\/div>\n","protected":false},"excerpt":{"rendered":"

\u5bf9\u4e8e\u5e7f\u5927\u79d1\u7814\u72d7\u800c\u8a00\uff0c\u5b9e\u9a8c\u7ed3\u679c\u7edd\u5bf9\u662f\u96be\u4ee5\u638c\u63a7\u7684\u4e8b\u60c5\u3002\u6240\u4ee5\uff0c\u4e3a\u4e86\u79d1\u7814\uff0c\u5404\u79cd\u7948\u6c42\u795e\u79d8\u529b\u91cf\u7684\u4f20\u8bf4\u5728\u5404\u4e2a\u5b9e\u9a8c\u5ba4\u4e4b\u95f4\u6d41\u4f20\uff0c […]<\/p>\n","protected":false},"author":1,"featured_media":126498,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[2],"tags":[],"_links":{"self":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/126497"}],"collection":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/comments?post=126497"}],"version-history":[{"count":1,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/126497\/revisions"}],"predecessor-version":[{"id":126509,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/126497\/revisions\/126509"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media\/126498"}],"wp:attachment":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media?parent=126497"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/categories?post=126497"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/tags?post=126497"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}