{"id":127558,"date":"2019-11-07T11:50:50","date_gmt":"2020-04-21T02:26:25","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/127558\/"},"modified":"2020-04-21T10:26:26","modified_gmt":"2020-04-21T02:26:26","slug":"f39169d676","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/127558\/","title":{"rendered":"\u79d1\u7814\u65b0\u624b\u8d44\u6599\u5408\u96c6 + \u5496\u5561\u5e74\u5361\u6253\u5305\u9001\u4e28\u6bcf\u4e2a\u6c89\u8ff7\u5b66\u672f\u7684\u65e5\u5b50\u90fd\u6709\u610f\u4e49"},"content":{"rendered":"
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<\/section>\n
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<\/section>\n<\/section>\n
\u6e29\u5ea6\u9aa4\u964d<\/strong>\uff0c<\/strong>\u8f6c\u773c\u95f4\u53c8\u5230\u4e86\u4e00\u5e74\u7684\u5c3e\u58f0\u2014\u2014<\/strong><\/span><\/section>\n

<\/strong><\/span><\/section>\n
\u60f3\u5fc5\u6211\u4eec\u61f5\u61c2\u7684\u79d1\u7814\u65b0\u624b\u5c0f\u767d\u4eec<\/span><\/section>\n
\u4e00\u5b9a\u5728\u5404\u4e2a\u5b9e\u9a8c\u5ba4\u91cc<\/span><\/section>\n
\u624b\u5fd9\u811a\u4e71\uff0c\u5175\u8352\u9a6c\u4e71\uff0c\u9e21\u98de\u72d7\u8df3\u7740<\/span><\/section>\n
\u5f53\u7136\uff0c\u4e5f\u5728\u6162\u6162\u6210\u957f\u7740\u3002<\/span><\/section>\n


<\/p>\n

\u300c \u5c0f\u84dd\u676f Vip \u5496\u5561\u5e74\u5361 \u300d<\/span><\/strong><\/span><\/section>\n
\u300c \u79d1\u7814\u65b0\u624b\u8d44\u6599\u5408\u96c6 60 G \u300d<\/span><\/strong><\/span><\/section>\n
\u9650\u65f6\u5305\u90ae\u514d\u8d39\u9001<\/span><\/strong><\/span><\/section>\n

<\/span><\/section>\n
\u8fd9\u4e2a\u53cc\u5341\u4e00\uff0c<\/span><\/section>\n
\u5c0f\u7f16\u4e5f\u60f3\u4e3a\u5927\u5bb6\u505a\u70b9\u4ec0\u4e48\u3002<\/span><\/section>\n
\u4e8e\u662f\u2026\u2026\u4e66\u9875\u548c\u5496\u5561\u90fd\u51c6\u5907\u5230\u4f4d\u4e86\uff01<\/span><\/section>\n
\u5e72\u676f\uff01\u7136\u540e\u4e00\u8d77\u5411\u524d\u8fc8\u8fdb\u5427\u3002<\/span><\/section>\n

<\/section>\n

<\/section>\n
– \u793c\u54c1\u5c55\u793a –<\/strong><\/span><\/section>\n


<\/p>\n

\"\u79d1\u7814\u65b0\u624b\u8d44\u6599\u5408\u96c6<\/section>\n

\"\u79d1\u7814\u65b0\u624b\u8d44\u6599\u5408\u96c6<\/p>\n


<\/p>\n


<\/p>\n

\u60ca\u559c\u4e00\uff1a\u745e\u5e78\u5496\u5561\u793c\u54c1\u5361<\/span><\/strong><\/span>
<\/p>\n


<\/p>\n

\u745e\u5e78\u5496\u5561\uff08\u5c0f\u84dd\u676f\uff0915 \u5f20\u996e\u54c1\u5238\uff0c\u5168\u5e74\u53ef\u7528\uff0c\u5373\u4f7f\u6bcf\u4e2a\u6708\u559d\u4e00\u676f\u90fd\u7528\u4e0d\u5b8c\u5462\u3002\u53ef\u4ee5\u5c1d\u904d\u5c0f\u84dd\u676f\u6240\u6709\u7684\u53e3\u5473\u5566\uff01\u8fd8\u80fd\u8bf7\u6574\u4e2a\u5b9e\u9a8c\u5ba4\u559d\u5496\u5561\uff0c\u79d2\u53d8\u5b9e\u9a8c\u5ba4\u56e2\u5ba0\u6709\u6ca1\u6709\uff01<\/span><\/section>\n

<\/section>\n
\u4ef7\u503c 360 \u5143<\/strong>\u7684\u793c\u54c1\u5361\uff0c\u4eca\u5929\u514d\u8d39\u9001\u7ed9\u5927\u5bb6\u5566\uff01<\/span><\/section>\n

<\/span><\/section>\n

<\/span><\/section>\n

\u60ca\u559c\u4e8c\uff1a<\/span><\/strong>\u79d1\u7814\u65b0\u624b\u8d44\u6599\u8d85\u5168\u5408\u96c6\uff0860 G\uff09<\/span><\/strong><\/strong><\/p>\n


<\/section>\n
\n
\n
\n
\n
\n
\u8d44\u6599\u5305\u6db5\u76d6\u4ee5\u4e0b\u5185\u5bb9\uff1a<\/span><\/strong>
<\/span><\/section>\n

<\/span><\/section>\n
\u4e00\u3001CRISPR\u6280\u672f<\/span><\/strong><\/span><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n
1\u3001\u89c6\u9891\u6559\u7a0b\uff08\u5f20\u950b\u89e3\u91ca\u4ec0\u4e48\u662fCISPR\uff0cCRISPR-Cas9\u9776\u70b9\u8bbe\u8ba1\u7b49\uff09<\/span>
<\/section>\n
2\u3001CRISPR \u5b9e\u9a8c\u6d41\u7a0b\uff08\u7ec6\u83cc\u3001\u52a8\u7269\u3001\u690d\u7269\uff09<\/span>
<\/section>\n
3\u3001CRISPR \u7ecf\u5178\u6587\u732e<\/span><\/section>\n
4\u3001\u5176\u5b83\u8865\u5145\u8d44\u6599<\/span><\/section>\n


<\/p>\n

\u4e8c\u3001PCR \u6280\u672f<\/span><\/strong><\/span><\/section>\n
1\u3001\u666e\u901aPCR<\/span><\/section>\n
2\u3001PCR-AFLP<\/span><\/section>\n
3\u3001PCR-SSCP<\/span><\/section>\n
4\u3001\u964d\u843dPCR<\/span><\/section>\n
5\u3001\u539f\u4f4dPCR<\/span><\/section>\n
6\u3001\u53cd\u5411PCR<\/span><\/section>\n
7\u3001PCR-RFLP<\/span><\/section>\n
8\u3001RT-PCR<\/span><\/section>\n
9\u3001\u91cd\u53e0\u5ef6\u4f38PCR<\/span><\/section>\n


<\/p>\n

\u4e09\u3001Western blot \u5b9e\u9a8c\u6280\u672f<\/span><\/strong><\/span>
<\/section>\n
1\u3001Western blot\u5b9e\u9a8c\u539f\u7406\u53ca\u64cd\u4f5c\u89c6\u9891<\/span><\/section>\n
2\u3001Western.blotting\u7ecf\u5178\u6587\u732e\u6c47\u603b<\/span><\/section>\n
3\u3001Western.blotting\u5b9e\u9a8c\u6307\u5bfc\u6d41\u7a0b<\/span><\/section>\n
4\u3001Western.blot\u86cb\u767d\u8d28\u5370\u8ff9\u5b9e\u9a8c\u539f\u7406\u4e0e\u64cd\u4f5c<\/span><\/section>\n


<\/p>\n

\u56db\u3001\u7ec6\u80de\u5b9e\u9a8c\u6280\u672f<\/span><\/strong><\/span>
<\/section>\n
1\u3001\u7ec6\u80de\u57f9\u517b\u76f8\u5173\u6587\u732e<\/span>
<\/section>\n
2\u3001\u7ec6\u80de\u57f9\u517b\u5fc5\u5907\u77e5\u8bc6<\/span><\/section>\n
3\u3001\u7ec6\u80de\u57f9\u517b\u5e38\u89c1\u95ee\u9898\u89e3\u7b54<\/span><\/section>\n
4\u3001\u64cd\u4f5c\u65b9\u6cd5<\/span>
<\/section>\n


<\/p>\n

\u4e94\u3001\u86cb\u767d\u8d28\u5b9e\u9a8c\u6280\u672f<\/span><\/strong><\/span><\/section>\n
1\u3001\u86cb\u767d\u8d28\u7684\u8868\u8fbe\u3001\u5206\u79bb\u3001\u7eaf\u5316\u5b9e\u9a8c<\/span><\/section>\n
2\u3001\u86cb\u767d\u8d28\u542b\u91cf\u7684\u6d4b\u5b9a<\/span>
<\/section>\n
3\u3001\u86cb\u767d\u8d28\u80f6\u7684\u5236\u5907<\/span>
<\/section>\n
4\u3001\u514d\u75ab\u7ec4\u5316\u5b9e\u9a8c<\/span>
<\/section>\n
5\u3001\u514d\u75ab\u5171\u6c89\u6dc0Co-IP\u5b9e\u9a8c\u64cd\u4f5c\u6b65\u9aa4<\/span>
<\/section>\n
6\u3001ELISA\u6b65\u9aa4\u3001\u8bd5\u5242\u53ca\u6ce8\u610f\u4e8b\u9879<\/span>
<\/section>\n


<\/p>\n

\u516d\u3001DNA\u5b9e\u9a8c\u6280\u672f<\/span><\/strong><\/span>
<\/section>\n
1\u3001\u8d28\u7c92DNA\u7684\u63d0\u53d6<\/span>
<\/section>\n
2\u3001\u91cd\u7ec4\u8d28\u7c92\u7684\u8fde\u63a5\u3001\u8f6c\u5316\u53ca\u7b5b\u9009<\/span><\/section>\n
3\u3001Southern Blot\u539f\u7406\u53ca\u8bd5\u9a8c\u65b9\u6cd5\u554a<\/span>
<\/section>\n
4\u3001\u57fa\u56e0\u5b9a\u70b9\u7a81\u53d8<\/span><\/section>\n
5\u3001\u5355\u6838\u82f7\u9178\u591a\u6001\u6027\uff08SNP\uff09\u5b9e\u9a8c<\/span>
<\/section>\n
6\u3001SSR\u5206\u5b50\u6807\u8bb0\u5b9e\u9a8c\u64cd\u4f5c\u53ca\u5176\u6ce8\u610f\u4e8b\u9879<\/span>
<\/section>\n


<\/p>\n

\u4e03\u3001RNA \u5b9e\u9a8c\u6280\u672f<\/span><\/strong><\/span>
<\/section>\n
1\u3001Trizol\u6cd5\u63d0\u53d6\u603bRNA<\/span><\/section>\n
2\u3001RNA \u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3<\/span>
<\/section>\n
3\u3001RNA\u7684\u5206\u79bb\u7eaf\u5316<\/span><\/section>\n
4\u3001Northern Blot \u539f\u7406\u53ca\u5b9e\u9a8c\u65b9\u6cd5<\/span>
<\/section>\n
5\u3001RNA\u5e72\u6270\u5b9e\u9a8c<\/span>
<\/section>\n
6\u3001siRNA\u8868\u8fbe\u8f7d\u4f53\u7684\u6784\u5efa<\/span>
<\/section>\n


<\/p>\n

\u516b\u3001\u5c0f\u9f20\u57fa\u672c\u64cd\u4f5c\u5408\u96c6<\/span><\/strong><\/span><\/section>\n
1\u3001\u5c0f\u9f20\u6807\u8bb0<\/span>
<\/section>\n
2\u3001\u5c0f\u9f20\u5904\u6b7b<\/span>
<\/section>\n
3\u3001\u5c0f\u9f20\u7ed9\u836f<\/span>
<\/section>\n
4\u3001\u5c0f\u9f20\u773c\u7403\u53d6\u8840<\/span>
<\/section>\n
5\u3001\u5c0f\u9f20\u6349\u62ff<\/span>
<\/section>\n


<\/p>\n

\u4e5d\u3001meta \u5206\u6790<\/span><\/strong><\/span>
<\/section>\n
1\u3001Meta \u5206\u6790\u96f6\u57fa\u7840\u5230\u7cbe\u901a\u8bfe\u7a0b<\/span>
<\/section>\n
2\u3001Meta \u5206\u6790\u8f6f\u4ef6\u64cd\u4f5c\u89c6\u9891\u6307\u5bfc<\/span>
<\/section>\n
3\u3001Meta \u5206\u6790\u4e13\u7528\u8f6f\u4ef6<\/span><\/section>\n
4\u3001Meta \u5206\u6790\u8d44\u6599\u5168\u96c6<\/span><\/section>\n
5\u3001 R\u8bed\u8a00\u4e0eMeta\u5206\u6790<\/span>
<\/section>\n


<\/p>\n

\u5341\u3001\u6570\u636e\u5206\u6790\u4e0eR\u8bed\u8a00<\/span><\/strong><\/span><\/section>\n
\u7cfb\u5217\u89c6\u9891\u6559\u7a0b\uff0b\u8bfe\u4ef6\uff0c\u4ef7\u503c588<\/span><\/section>\n


<\/p>\n

<\/section>\n
\u5341\u4e00\u3001\u5355\u4e2a\u57fa\u56e0\u751f\u7269\u4fe1\u606f\u5b66\u5206\u6790\uff08\u89c6\u9891\u6559\u7a0b\uff09<\/span><\/strong><\/section>\n
1\u3001\u57fa\u56e0\u7ed3\u6784\u9884\u6d4b\u548c\u529f\u80fd\u6ce8\u91ca<\/span><\/section>\n
2\u3001\u86cb\u767d\u7ed3\u6784\u57df\u9884\u6d4b
<\/span><\/section>\n
3\u3001\u540c\u6e90\u57fa\u56e0\u5206\u6790
<\/span><\/section>\n
4\u3001\u591a\u5e8f\u5217\u6bd4\u5bf9<\/span><\/section>\n
5\u3001\u86cb\u767d\u4e8c\u7ea7\u4e09\u7ea7\u7ed3\u6784\u9884\u6d4b
<\/span><\/section>\n
6\u3001\u86cb\u767d\u7279\u6027\u5206\u6790
<\/span><\/section>\n
7\u3001\u8de8\u819c\u7ed3\u6784\u5206\u6790
<\/span><\/section>\n
8\u3001\u4fe1\u53f7\u80bd\u5206\u6790
<\/span><\/section>\n
9\u3001\u4e9a\u7ec6\u80de\u5b9a\u4f4d\u9884\u6d4b
<\/span><\/section>\n
10\u3001\u542f\u52a8\u5b50\u5206\u6790
<\/span><\/section>\n
11\u3001\u8c03\u63a7\u76ee\u7684\u57fa\u56e0\u7684miRNA\u9884\u6d4b
<\/span><\/section>\n
12\u3001\u57fa\u56e0\u8868\u8fbe\u5206\u6790
<\/span><\/section>\n


<\/p>\n

\u5341\u4e8c\u3001\u5341\u5927\u5b9e\u9a8c\u6280\u672f\u8d44\u6e90\u5206\u4eab\u4e66\u7c4d<\/span><\/strong><\/section>\n
1\u3001\u9aa8\u79d1\u5e38\u7528\u8bca\u7597\u6280\u672f<\/span><\/section>\n
2\u3001\u514d\u75ab\u7ec4\u7ec7\u5316\u5b66\u5b9e\u9a8c\u6280\u672f\u53ca\u5e94\u7528
<\/span><\/section>\n
3\u3001\u795e\u7ecf\u751f\u7269\u5b66\u5b9e\u9a8c\u539f\u7406\u4e0e\u6280\u672f
<\/span><\/section>\n
4\u3001\u73b0\u4ee3\u5206\u5b50\u751f\u7269\u5b66\u6280\u672f\u548c\u5b9e\u9a8c\u6280\u5de7
<\/span><\/section>\n
5\u3001\u7ec6\u80de\u751f\u7269\u5b66\u5b9e\u9a8c\u6280\u672f
<\/span><\/section>\n
6\u3001\u533b\u5b66\u514d\u75ab\u5b66\u5e38\u7528\u5b9e\u9a8c\u6280\u672f
<\/span><\/section>\n
7\u3001\u75c5\u7406\u8bca\u65ad\u514d\u75ab\u7ec4\u5316\u624b\u518c
<\/span><\/section>\n
8\u3001\u533b\u5b66\u5b9e\u9a8c\u6280\u672f\u539f\u7406\u4e0e\u9009\u62e9\u7b2c\u4e8c\u7248
<\/span><\/section>\n
9\u3001\u7ec4\u7ec7\u548c\u7ec6\u80de\u57f9\u517b\u6280\u672f\u7b2c\u4e09\u7248<\/span><\/section>\n
10\u3001\u4e2d\u836f\u5236\u5242\u5206\u6790\u5b9e\u9a8c\u6280\u672f<\/span><\/section>\n

<\/section>\n
\u90e8\u5206\u8d44\u6599\u9884\u89c8\u56fe<\/span><\/strong>\"\u79d1\u7814\u65b0\u624b\u8d44\u6599\u5408\u96c6<\/section>\n

<\/span><\/strong><\/section>\n

\"\u79d1\u7814\u65b0\u624b\u8d44\u6599\u5408\u96c6<\/p>\n


<\/p>\n

\u7785\u7785\uff0c\u70ed\u95e8\u7684\u5b9e\u9a8c\u6280\u672f\u57fa\u672c\u90fd\u5728\u8fd9 60G \u91cc\u9762\u4e86\u3002\u6536\u85cf\u8d77\u6765\uff0c\u6307\u4e0d\u5b9a\u54ea\u5929\u5c31\u7528\u5230\u4e86\u3002<\/span>\u521d\u5b66\u8005\u4eec\u901f\u901f<\/span>\u5b89\u6392\u4e0a\u5427\uff01\u4e00\u8fb9\u559d\u5496\u5561\uff0c\u4e00\u8fb9\u641e\u5b66\u672f\uff0c\u8fd9\u6837\u7684\u65e5\u5b50\u4e5f\u597d\u50cf\u4e5f\u4e0d\u8d56\u54c8\uff01<\/span><\/p>\n


<\/p>\n


<\/p>\n

\u600e\u4e48\u9886\u53d6\u7cbe\u5fc3\u51c6\u5907\u7684\u8d44\u6599\u548c\u5496\u5561\u5462\uff1f<\/span><\/strong><\/span><\/section>\n
\n
\n
\n

<\/section>\n
\u6d3b\u52a8\u65f6\u95f4\uff1a<\/span><\/strong>\u5373\u523b\u8d77 \u81f3 <\/span>2019 \u5e74 11 \u6708 10 \u65e5 23:00<\/span><\/section>\n


<\/p>\n

\n
\n
\n
\u9886\u53d6\u65b9\u5f0f\u5982\u4e0b\uff1a<\/span><\/strong><\/section>\n

<\/span><\/strong><\/section>\n
\"\u79d1\u7814\u65b0\u624b\u8d44\u6599\u5408\u96c6<\/section>\n
\u79d1\u7814\u8d44\u6599\u5305\u548c\u5496\u5561\u5e74\u5361\u9886\u53d6\u65b9\u5f0f<\/span><\/strong><\/a>
<\/section>\n
\uff08<\/span>\"\u79d1\u7814\u65b0\u624b\u8d44\u6599\u5408\u96c6\u6233\u6233\u770b\uff09<\/span>
<\/section>\n
\n
\n
\n


<\/p>\n

\u53d1\u8d27\u65f6\u95f4<\/span><\/strong>\uff1a\u793c\u54c1\u5361\u5c06\u4e8e\u6d3b\u52a8\u7ed3\u675f\u540e 3 \u4e2a\u5de5\u4f5c\u65e5\u5185\uff0c\u7edf\u4e00\u5b89\u6392\u5bc4\u9001\u3002<\/span><\/section>\n<\/section>\n<\/section>\n


<\/section>\n

\u5ba2\u670d\u5fae\u4fe1<\/span><\/strong>\uff1a\u67da\u5b50\u9171\uff08Lj02319\uff09<\/span><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section><\/div>\n","protected":false},"excerpt":{"rendered":"

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