{"id":18640,"date":"2018-07-30T01:00:00","date_gmt":"2018-08-08T01:05:59","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/2018\/08\/08\/%e5%bd%93%e8%b4%a8%e8%b0%b1%e6%8a%80%e6%9c%af%e5%ba%94%e7%94%a8%e4%ba%8e%e5%8c%bb%e5%ad%a6%e6%a3%80%e9%aa%8c\/"},"modified":"2018-08-08T09:06:01","modified_gmt":"2018-08-08T01:06:01","slug":"%e5%bd%93%e8%b4%a8%e8%b0%b1%e6%8a%80%e6%9c%af%e5%ba%94%e7%94%a8%e4%ba%8e%e5%8c%bb%e5%ad%a6%e6%a3%80%e9%aa%8c","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/18640\/","title":{"rendered":"\u5f53\u8d28\u8c31\u6280\u672f\u5e94\u7528\u4e8e\u533b\u5b66\u68c0\u9a8c\u2026\u2026"},"content":{"rendered":"
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\u8d28\u8c31\u6280\u672f (mass spectrometry) \u662f\u5206\u79bb\u548c\u68c0\u6d4b\u5e26\u7535\u7c92\u5b50\u8d28\u8377\u6bd4\u7684\u5206\u6790\u6280\u672f\u3002\u968f\u7740\u79bb\u5b50\u6e90\u53ca\u8d28\u91cf\u5206\u6790\u5668\u6280\u672f\u7684\u53d8\u9769\u3001\u8d28\u8c31\u4eea\u5668\u8bbe\u8ba1\u7684\u5feb\u901f\u6539\u8fdb\u7b49\uff0c\u8d28\u8c31\u6280\u672f\u5df2\u6210\u4e3a\u5316\u5b66\u5206\u6790\u9886\u57df\u548c\u751f\u547d\u79d1\u5b66\u9886\u57df\u975e\u5e38\u6709\u6548\u7684\u5206\u6790\u5de5\u5177\uff0c\u5c24\u5176\u5728\u533b\u5b66\u68c0\u9a8c\u4e2d\u7684\u5e94\u7528\u8d8a\u6765\u8d8a\u4e3a\u5e7f\u6cdb\u548c\u6df1\u5165\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n


<\/p>\n

\"\u5f53\u8d28\u8c31\u6280\u672f\u5e94\u7528\u4e8e\u533b\u5b66\u68c0\u9a8c\u2026\u2026\"<\/p>\n


<\/p>\n

\u7531\u4e8e\u8d28\u8c31\u6280\u672f\u7684\u9ad8\u7279\u5f02\u6027\u3001\u9ad8\u7075\u654f\u5ea6\u3001\u5355\u6b21\u5206\u6790\u7684\u5feb\u901f\u6027\u4e0e\u68c0\u6d4b\u4fe1\u606f\u7684\u4e30\u5bcc\u6027\uff0c\u4ee5\u53ca\u5bf9\u590d\u6742\u751f\u7269\u57fa\u8d28\u5206\u6790\u7684\u9ad8\u8010\u53d7\u6027\u7b49\u7279\u70b9\uff0c\u4e34\u5e8a\u7814\u7a76\u548c\u8bca\u65ad\u5de5\u4f5c\u4e5f\u9010\u6e10\u501a\u91cd\u4e8e\u6b64\u7c7b\u91cd\u8981\u7684\u65b0\u578b\u68c0\u6d4b\u6280\u672f\u3002\u5982\uff1a\u8d28\u8c31\u6280\u672f\u6240\u80fd\u63d0\u4f9b\u7684\u4e30\u5bcc\u7684\u68c0\u6d4b\u4fe1\u606f\uff0c\u6709\u52a9\u4e8e\u4e34\u5e8a\u66f4\u52a0\u5b8c\u6574\u5730\u4e86\u89e3\u75be\u75c5\u548c\u75c5\u7406\u72b6\u6001\uff0c\u4ece\u800c\u4e3a\u60a3\u8005\u63d0\u4f9b\u66f4\u4e3a\u5168\u9762\u548c\u51c6\u786e\u7684\u8bca\u7597\u670d\u52a1\u3002<\/span><\/p>\n


<\/p>\n

\u5728\u7f8e\u56fd\u7b49\u53d1\u8fbe\u56fd\u5bb6\uff0c\u8d28\u8c31\u6280\u672f\u5df2\u5e7f\u6cdb\u5e94\u7528\u4e8e\u533b\u5b66\u68c0\u9a8c\uff0c\u57fa\u4e8e\u8be5\u6280\u672f\u5f00\u53d1\u51fa\u7684\u4e34\u5e8a\u68c0\u6d4b\u9879\u76ee\u5df2\u6709\u6570\u767e\u9879\uff0c\u4f46\u6211\u56fd\u76ee\u524d\u4ecd\u5904\u4e8e\u8d77\u6b65\u9636\u6bb5\uff0c\u68c0\u6d4b\u9879\u76ee\u670970\u4f59\u9879\uff1b\u5e94\u7528\u8986\u76d6\u9762\u975e\u5e38\u5e7f\u6cdb\uff0c\u6db5\u76d6\u4e86\u7f55\u89c1\u548c\u9ad8\u96be\u5ea6\u5206\u6790\uff0c\u5305\u62ec\u5fae\u751f\u7269\u9274\u5b9a\u3001\u751f\u5316\u68c0\u9a8c\uff08\u6fc0\u7d20\u68c0\u6d4b\u3001\u836f\u7269\u6d53\u5ea6\u76d1\u6d4b\u3001\u9057\u4f20\u6027\u75be\u75c5\u68c0\u6d4b\u3001\u8425\u517b\u7d20\u68c0\u6d4b\u7b49) \u548c\u5206\u5b50\u751f\u7269\u8bca\u65ad (\u86cb\u767d\u7ec4\u5b66\u3001\u6838\u82f7\u9178\u591a\u6001\u6027\u3001\u4ee3\u8c22\u7ec4\u5b66) [1-5]<\/sup>\uff1b\u5e94\u7528\u8303\u56f4\u4e5f\u5728\u9010\u6b65\u6269\u5c55\uff0c\u4ece\u751f\u5316\u68c0\u9a8c\u3001\u5fae\u751f\u7269\u9274\u5b9a\uff0c\u5230\u4ee3\u8c22\u7ec4\u5b66\u3001\u8102\u8d28\u7ec4\u5b66\u3001\u86cb\u767d\u7ec4\u5b66\uff0c\u518d\u5230\u53c2\u8003\u6d4b\u91cf\u7a0b\u5e8f\u7684\u5efa\u7acb\u548c\u6821\u51c6\u54c1\u8d4b\u503c\uff0c\u4e43\u81f3\u672f\u4e2d\u5e94\u7528\u53ca\u5e8a\u65c1\u68c0\u6d4b\u3002<\/span><\/p>\n


<\/p>\n

\u672c\u6587\u5c06\u5168\u9762\u9610\u8ff0\u8d28\u8c31\u6280\u672f\u5728\u533b\u5b66\u5b9e\u9a8c\u5ba4\u4e2d\u7684\u53d1\u5c55\u5386\u7a0b\uff0c\u4ee5\u53ca\u5728\u533b\u5b66\u68c0\u9a8c\u9886\u57df\u7684\u4e3b\u8981\u5e94\u7528\u60c5\u51b5\u548c\u7279\u70b9\uff0c\u540c\u65f6\u5256\u6790\u4e86\u8d28\u8c31\u6280\u672f\u76ee\u524d\u5728\u4e34\u5e8a\u5e94\u7528\u4e2d\u4e3b\u8981\u75db\u70b9\u548c\u672a\u6765\u53ef\u80fd\u7684\u53d1\u5c55\u65b9\u5411\uff0c\u5e0c\u671b\u80fd\u8f83\u4e3a\u5168\u9762\u5730\u7efc\u8ff0\u8d28\u8c31\u6280\u672f\u4e34\u5e8a\u5e94\u7528\u7684\u73b0\u72b6\u4e0e\u672a\u6765\u3002<\/span><\/p>\n

\n
\n
\u4e00\u3001\u8d28\u8c31\u6280\u672f\u5728\u533b\u5b66\u5b9e\u9a8c\u5ba4\u4e2d\u7684\u53d1\u5c55<\/span><\/strong><\/span><\/section>\n
<\/section>\n<\/section>\n<\/section>\n

\u4ece\u8d28\u8c31\u6280\u672f\u6700\u521d\u8fdb\u5165\u533b\u5b66\u68c0\u9a8c\u9886\u57df\uff0c\u81f3\u4eca\u53d1\u5c55\u81f3\u591a\u9886\u57df\u3001\u5bbd\u8303\u56f4\u7684\u5e94\u7528\uff0c\u5927\u7ea6\u7ecf\u5386\u4e86\u56db\u5341\u5e74\u7684\u65f6\u95f4\uff0c\u6700\u65e9\u59cb\u4e8e20\u4e16\u7eaa80\u5e74\u4ee3\u3002\u7531\u4e8e\u514d\u75ab\u6cd5\u68c0\u6d4b\u5b58\u5728\u5047\u9633\u6027\uff0c\u8d28\u8c31\u6280\u672f\u5f00\u59cb\u4ece\u7814\u7a76\u6027\u5b9e\u9a8c\u5ba4\u8d70\u5165\u4e34\u5e8a\u5b9e\u9a8c\u5ba4\uff0c\u5982\u6c14\u76f8\u8272\u8c31\u8d28\u8c31 (gas chromatography mass spectrometry, GC-MS) \u6280\u672f\u8d77\u521d\u5e94\u7528\u4e8e\u519b\u4e8b\u836f\u7269\u76d1\u6d4b\u3002\u63a8\u52a8\u8fd9\u79cd\u8f6c\u53d8\u51fa\u73b0\u7684\u662f\u53d1\u751f\u57281981\u5e74\u7684\u7f8e\u56fd\u5c3c\u7c73\u5179\u53f7\u822a\u7a7a\u6bcd\u8230\u4e8b\u6545\u3002\u5c3c\u7c73\u5179\u53f7\u662f\u7f8e\u56fd\u6d77\u519b\u4e2d\u6700\u5927\u7684\u4e00\u8258\u6838\u52a8\u529b\u822a\u7a7a\u6bcd\u8230\uff0c1981\u5e745\u670825\u65e5\u6df1\u591c\uff0c\u5c3c\u7c73\u5179\u53f7\u5728\u51c6\u5907\u56de\u6536\u6a21\u62df\u4f5c\u6218\u5f52\u6765\u7684\u673a\u7fa4\u65f6\u53d1\u751f\u610f\u5916\uff0c\u5f15\u53d1\u5927\u706b\uff0c\u8be5\u4e8b\u6545\u6700\u7ec8\u9020\u6210\u591a\u4eba\u6b7b\u4f24\u3002\u968f\u540e\u91c7\u7528\u514d\u75ab\u6cd5\u68c0\u6d4b\u98de\u884c\u5458\u5c3f\u6db2\u6837\u672c\u65f6\u53d1\u73b0\uff0c\u5927\u90e8\u5206\u5c3f\u68c0\u7ed3\u679c\u4e2d\u5927\u9ebb\u4ee3\u8c22\u7269\u5448\u9633\u6027\uff0c\u8868\u660e\u519b\u961f\u4e2d\u53ef\u80fd\u5b58\u5728\u836f\u7269\u6ee5\u7528\u60c5\u51b5\u3002<\/span><\/p>\n


<\/p>\n

\u56e0\u514d\u75ab\u6cd5\u68c0\u6d4b\u7ed3\u679c\u672c\u8eab\u5b58\u5728\u8f83\u9ad8\u7684\u5047\u9633\u6027\u7387\uff0c\u6240\u4ee5\u9700\u8981\u4f7f\u7528\u66f4\u4e3a\u7279\u5f02\u7684GC-MS\u65b9\u6cd5\u8fdb\u884c\u786e\u8ba4\u3002\u800cGC-MS\u5728\u5e94\u7528\u540e\u768410\u5e74\u91cc\u4f7f\u5927\u9ebb\u68c0\u6d4b\u9633\u6027\u7387\u4ece18%\u964d\u4f4e\u81f38%\uff0c\u8fd9\u4e5f\u4fc3\u4f7f\u4e86\u8d28\u8c31\u6280\u672f\u8fdb\u5165\u6bd2\u7406\u5b9e\u9a8c\u5ba4\u5e76\u5e94\u7528\u4e8e\u6ee5\u7528\u836f\u7269\u68c0\u6d4b\u548c\u6cbb\u7597\u836f\u7269\u76d1\u6d4b\uff0c\u4e34\u5e8a\u8d28\u8c31\u68c0\u6d4b\u5f00\u59cb\u840c\u82bd\u30021988\u5e74\uff0c\u7f8e\u56fd\u8054\u90a6\u836f\u54c1\u68c0\u9a8c\u5c40\u53d1\u5e03\u5f3a\u5236\u6027\u6307\u5357\uff0c\u8981\u6c42\u6cbb\u7597\u836f\u7269\u76d1\u6d4b\u5fc5\u987b\u4f7f\u7528\u8d28\u8c31\u6cd5\u8fdb\u884c\u786e\u8ba4\uff0c\u5960\u5b9a\u4e86\u8d28\u8c31\u6280\u672f\u5728\u6cbb\u7597\u836f\u7269\u76d1\u6d4b\u4e2d\u7684\u91cd\u8981\u5730\u4f4d[2]<\/sup>\u3002<\/span><\/p>\n


<\/p>\n

\u968f\u7740GC-MS\u5728\u533b\u5b66\u68c0\u9a8c\u9886\u57df\u5e94\u7528\u7684\u9010\u6b65\u589e\u591a\uff0c\u514d\u75ab\u6cd5\u7528\u4e8e\u7c7b\u56fa\u9187\u6fc0\u7d20\u68c0\u6d4b\u7684\u7f3a\u70b9\u4e5f\u65e5\u76ca\u51f8\u663e\uff0c\u5c24\u5176\u662f\u5728\u6d4b\u5b9a\u5987\u5973\u548c\u513f\u7ae5\u4f53\u5185\u4f4e\u6d53\u5ea6\u777e\u916e\u65f6\u3002\u4f46\u56e0GC-MS\u4e3b\u8981\u9002\u7528\u4e8e\u6325\u53d1\u6027\u548c\u70ed\u7a33\u5b9a\u6027\u5316\u5408\u7269\uff0c\u6837\u54c1\u5236\u5907\u7a0b\u5e8f\u590d\u6742\uff0c\u68c0\u6d4b\u901a\u91cf\u4f4e\uff0c\u56e0\u6b64\u9650\u5236\u4e86\u5176\u5728\u4e34\u5e8a\u4e2d\u7684\u5e94\u7528\u300220\u4e16\u7eaa80\u5e74\u4ee3\uff0c\u5feb\u539f\u5b50\u8f70\u51fb\u3001\u7535\u55b7\u96fe\u548c\u8f85\u52a9\u6fc0\u5149\u89e3\u6790\u7b49\u201c\u8f6f\u7535\u79bb\u201d\u6280\u672f\u7684\u53d1\u5c55\uff0c\u4f7f\u86cb\u767d\u8d28\u3001\u9176\u3001\u6838\u9178\u7b49\u751f\u7269\u5927\u5206\u5b50\u7684\u68c0\u6d4b\u6210\u4e3a\u53ef\u80fd\uff0c\u5927\u5927\u62d3\u5c55\u4e86\u8d28\u8c31\u6280\u672f\u5728\u533b\u5b66\u68c0\u9a8c\u9886\u57df\u7684\u5e94\u7528\u8303\u56f4[1]<\/sup>\u3002<\/span><\/p>\n


<\/p>\n

20\u4e16\u7eaa90\u5e74\u4ee3\uff0c\u4e32\u8054\u8d28\u8c31\u6280\u672f\u5f00\u59cb\u5e94\u7528\u4e8e\u65b0\u751f\u513f\u7b5b\u67e5\u3002\u800c\u7535\u55b7\u96fe\u7535\u79bb\u63a5\u53e3\u6280\u672f (electrospray ionization interface technology, ESI) \u53d1\u5c55\u4f7f\u5f97\u4e24\u79cd\u5f3a\u6709\u529b\u7684\u5206\u6790\u5de5\u5177\u2014\u6db2\u76f8\u7cfb\u7edf\u548c\u8d28\u8c31\u7cfb\u7edf\u7684\u7ed3\u5408\u6210\u4e3a\u4e86\u53ef\u80fd\uff0c\u4e24\u8005\u7684\u7ed3\u5408\u4e5f\u8fdb\u4e00\u6b65\u4fc3\u8fdb\u4e86\u6db2\u76f8\u8272\u8c31-\u4e32\u8054\u8d28\u8c31 (liquid chromatography-tandem mass spectrometry, LC-MS\/MS) \u6280\u672f\u5728\u533b\u5b66\u68c0\u9a8c\u3001\u4e34\u5e8a\u7814\u7a76\u53ca\u75be\u75c5\u8bca\u65ad\u7684\u5e94\u7528\u53d1\u5c55\u3002\u6db2\u76f8\u8272\u8c31\u8d28\u8c31\u6280\u672f\u7684\u8054\u7528\u80fd\u591f\u4f7f\u975e\u6325\u53d1\u6027\u548c\u70ed\u4e0d\u7a33\u5b9a\u751f\u7269\u5206\u5b50\u7535\u79bb\u5e76\u5728\u6781\u4f4e\u7684\u6d53\u5ea6\u4e0b\u5f97\u5230\u68c0\u6d4b\uff0c\u7b80\u5316\u4e86\u6837\u54c1\u5236\u5907\u6d41\u7a0b\uff0c\u63d0\u9ad8\u4e86\u68c0\u6d4b\u901a\u91cf\uff0c\u6781\u5927\u7f29\u77ed\u4e86\u62a5\u544a\u5468\u671f\uff0c\u56e0\u800c\u5728\u4e34\u5e8a\u5b9e\u9a8c\u5ba4\u7684\u5e38\u89c4\u68c0\u6d4b\u5de5\u4f5c\u4e2d\u5f97\u5230\u4e86\u8fc5\u731b\u7684\u53d1\u5c55\u3002<\/span><\/p>\n


<\/p>\n

21\u4e16\u7eaa\u521d\u671f\uff0c\u8d28\u8c31\u6280\u672f\u5f00\u59cb\u5c1d\u8bd5\u5e94\u7528\u4e8e\u611f\u67d3\u6027\u75be\u75c5\u7684\u68c0\u6d4b\uff0c\u5982\u8840\u6e90\u6027\u611f\u67d3\u75be\u75c5\u7684\u5206\u5b50\u8bca\u65ad\u3002\u57fa\u8d28\u8f85\u52a9\u6fc0\u5149\u89e3\u5438\u7535\u79bb\u6280\u672f (matrix-assisted laser desorption\/ionization, MALDI) \u7684\u53d1\u5c55\u5219\u5b8c\u7f8e\u5730\u5b9e\u73b0\u4e86\u751f\u7269\u5927\u5206\u5b50\u7684\u8f6f\u79bb\u5b50\u5316\uff0c\u901a\u8fc7\u5f15\u5165\u57fa\u8d28\u5206\u5b50\uff0c\u4f7f\u5f85\u6d4b\u5206\u5b50\u4e0d\u4ea7\u751f\u788e\u7247\uff0c\u89e3\u51b3\u4e86\u975e\u6325\u53d1\u6027\u548c\u70ed\u4e0d\u7a33\u5b9a\u6027\u751f\u7269\u5927\u5206\u5b50\u89e3\u5438\u79bb\u5b50\u5316\u7684\u95ee\u9898\uff0c\u4fbf\u6377\u5730\u5c06\u751f\u7269\u6837\u672c\u5f15\u5165\u8d28\u8c31\u7cfb\u7edf\uff0c\u5e76\u7ed3\u5408\u98de\u884c\u65f6\u95f4\u8d28\u91cf\u5206\u6790\u5668 (time-of-flight, TOF) \u6280\u672f\uff0c\u5b9e\u73b0\u4e86\u5fae\u751f\u7269\u7684\u5feb\u901f\u9274\u5b9a\u5206\u6790\uff0c\u9274\u5b9a\u65f6\u95f4\u53ef\u7f29\u77ed1.45d\u3002\u76f8\u5bf9\u4e8e\u4f20\u7edf\u7684\u5fae\u751f\u7269\u68c0\u6d4b\u65b9\u6cd5\uff0cMALDI-TOF\u53ef\u8282\u7701\u4eba\u529b\u548c\u65f6\u95f4\uff0c\u8be5\u5e94\u7528\u4e5f\u4fc3\u8fdb\u4e86\u8d28\u8c31\u6280\u672f\u5728\u5927\u5206\u5b50\u68c0\u6d4b\u9886\u57df\u7684\u5e7f\u6cdb\u4f7f\u7528\uff0c\u5c06\u8d28\u8c31\u7684\u5e94\u7528\u63a8\u4e0a\u4e86\u4e00\u4e2a\u65b0\u7684\u53f0\u9636\u3002<\/span><\/p>\n


<\/p>\n

2013\u5e74\uff0c\u7f8e\u56fd\u98df\u54c1\u836f\u54c1\u76d1\u7763\u7ba1\u7406\u5c40 (Food and Drug Administration, FDA) \u9996\u6b21\u8ba4\u53ef\u4f7f\u7528MALDI-TOF\u5bf9\u5fae\u751f\u7269\u8fdb\u884c\u9274\u5b9a\u3002\u53e6\u5916\uff0c\u8fd1\u51e0\u5e74\u8fd8\u51fa\u73b0\u4e86\u5c06\u8d28\u8c31\u6280\u672f\u7528\u4e8e\u5b9e\u65f6\u6307\u5bfc\u764c\u75c7\u5916\u79d1\u624b\u672f\u7684\u524d\u6cbf\u5e94\u7528[2,5]<\/sup>\u3002<\/span><\/p>\n


<\/p>\n

\u5728\u6211\u56fd\uff0c\u8d28\u8c31\u6280\u672f\u5728\u533b\u5b66\u68c0\u9a8c\u4e2d\u7684\u5e94\u7528\u6700\u65e9\u4e5f\u59cb\u4e8e\u6cbb\u7597\u836f\u7269\u76d1\u6d4b\u3002\u968f\u540e\uff0c\u8d28\u8c31\u6280\u672f\u5728\u9057\u4f20\u4ee3\u8c22\u75c5\u68c0\u6d4b\u3001\u8425\u517b\u7d20\u68c0\u6d4b\u548c\u5fae\u751f\u7269\u9274\u5b9a\u5de5\u4f5c\u4e2d\u4f5c\u51fa\u7684\u7a81\u51fa\u8d21\u732e\uff0c\u5f15\u8d77\u884c\u4e1a\u5185\u7684\u5e7f\u6cdb\u5173\u6ce8\u3002\u540c\u65f6\u5176\u5728\u86cb\u767d\u7ec4\u5b66\u7b49\u7814\u7a76\u9886\u57df\u4e5f\u5177\u6709\u826f\u597d\u7684\u524d\u666f\uff0c\u6709\u671b\u6210\u4e3a\u533b\u5b66\u68c0\u9a8c\u9886\u57df\u7ee7\u57fa\u56e0\u6d4b\u5e8f\u6280\u672f\u4e4b\u540e\u7684\u4e0b\u4e00\u4e2a\u9769\u547d\u6027\u6280\u672f\u3002<\/span><\/p>\n


<\/p>\n

\n
\n
\u4e8c\u3001\u8d28\u8c31\u6280\u672f\u5728\u533b\u5b66\u68c0\u9a8c\u4e2d\u7684\u4e3b\u8981\u5e94\u7528<\/span><\/strong><\/span><\/section>\n
<\/section>\n<\/section>\n<\/section>\n


<\/p>\n

1\u3001<\/strong>\u8d28\u8c31\u6280\u672f\u5728\u4e34\u5e8a\u751f\u5316\u68c0\u9a8c\u4e2d\u7684\u5e94\u7528<\/strong><\/span><\/p>\n

\u8d28\u8c31\u6280\u672f\u5728\u5e94\u7528\u8f83\u65e9\u7684\u56fd\u5bb6\u5df2\u6210\u4e3a\u7ee7\u514d\u75ab\u5b66\u65b9\u6cd5\u548c\u5316\u5b66\u53d1\u5149\u6cd5\u4e4b\u540e\u7684\u7b2c\u4e09\u5927\u751f\u5316\u68c0\u6d4b\u6280\u672f\u3002\u76ee\u524d\u91c7\u7528\u8d28\u8c31\u6280\u672f\u68c0\u6d4b\u7684\u9879\u76ee\u6570\u91cf\u867d\u7136\u4e0e\u5176\u4ed6\u4e24\u79cd\u65b9\u6cd5\u76f8\u6bd4\u8fd8\u6709\u5f88\u5927\u5dee\u8ddd\uff0c\u4f46\u8d8a\u6765\u8d8a\u591a\u7684\u751f\u5316\u68c0\u6d4b\u9879\u76ee\u6b63\u88ab\u8f6c\u79fb\u81f3\u8d28\u8c31\u6280\u672f\u5e73\u53f0\u8fdb\u884c\u68c0\u6d4b\uff1b\u8d28\u8c31\u6280\u672f\u4e5f\u6210\u4e3a\u751f\u5316\u68c0\u9a8c\u9886\u57df\u65b0\u5174\u7684\u53d1\u5c55\u65b9\u5411\u548c\u4e0d\u53ef\u6216\u7f3a\u7684\u91cd\u8981\u6280\u672f[6]<\/sup>\u3002<\/span><\/p>\n


<\/p>\n

\u8d28\u8c31\u6280\u672f\u5728\u4e34\u5e8a\u751f\u5316\u68c0\u9a8c\u4e2d\u5e94\u7528\u6700\u4e3a\u6210\u719f\u7684\u9879\u76ee\u4e3b\u8981\u5305\u62ec\uff1a\u751f\u5316\u9057\u4f20\u68c0\u6d4b\u3001\u6cbb\u7597\u836f\u7269\u76d1\u6d4b\u3001\u7c7b\u56fa\u9187\u6fc0\u7d20\u68c0\u6d4b\u3001\u8425\u517b\u7d20\u68c0\u6d4b\u4ee5\u53ca\u6bd2\u7406\u5b66\u68c0\u6d4b\u3002\u6280\u672f\u9ad8\u7279\u5f02\u6027\u7684\u7279\u70b9\u53ef\u6709\u6548\u907f\u514d\u7ed3\u6784\u7c7b\u4f3c\u7269\u5bf9\u68c0\u6d4b\u7ed3\u679c\u7684\u5f71\u54cd\uff0c\u4e3a\u4e34\u5e8a\u63d0\u4f9b\u66f4\u51c6\u786e\u7684\u7ed3\u679c\uff0c\u63d0\u9ad8\u60a3\u8005\u7684\u4f9d\u4ece\u6027\u3002\u6280\u672f\u9ad8\u7075\u654f\u5ea6\u7684\u7279\u70b9\u53ef\u5728\u5f88\u5927\u7a0b\u5ea6\u4e0a\u5f25\u8865\u5185\u5206\u6ccc\u7c7b\u56fa\u9187\u6fc0\u7d20\u68c0\u6d4b\u4e2d\uff0c\u4f4e\u6d53\u5ea6\u5316\u5408\u7269\u68c0\u6d4b\u56f0\u96be\u548c\u6d4b\u4e0d\u51c6\u7684\u96be\u9898\uff0c\u4e3a\u75be\u75c5\u7684\u9884\u6d4b\u548c\u8bca\u7597\u5206\u578b\u63d0\u4f9b\u51c6\u786e\u7ed3\u679c\u3002<\/span><\/p>\n


<\/p>\n

\u56fd\u5916\u8bb8\u591a\u5185\u5206\u6ccc\u5b9e\u9a8c\u5ba4\u5df2\u7ecf\u5c06\u5927\u90e8\u5206\u4f53\u5185\u6fc0\u7d20\u7c7b\u7269\u8d28\u7684\u68c0\u6d4b\u7531\u653e\u5c04\u514d\u75ab\u5b66\u65b9\u6cd5\u6216\u514d\u75ab\u5b66\u65b9\u6cd5\u8f6c\u6362\u4e3aLC-MS\/MS\u65b9\u6cd5\uff0c\u5e76\u5c06\u8d28\u8c31\u6280\u672f\u4f5c\u4e3a\u5185\u5206\u6ccc\u7c7b\u56fa\u9187\u6fc0\u7d20\u7c7b\u7269\u8d28\u68c0\u6d4b\u7684\u9996\u9009\u65b9\u6cd5\u3002\u8d28\u8c31\u6280\u672f\u4e00\u6b21\u53ef\u68c0\u6d4b\u591a\u79cd\u5316\u5408\u7269\u7684\u7279\u70b9\uff0c\u53ef\u63d0\u9ad8\u68c0\u6d4b\u901a\u91cf\u3001\u51cf\u5c11\u6837\u54c1\u7528\u91cf\u548c\u964d\u4f4e\u68c0\u6d4b\u6210\u672c\u3002\u5982\u5728\u751f\u5316\u9057\u4f20\u68c0\u6d4b\u4e2d\uff0c\u8d28\u8c31\u6280\u672f\u4e00\u6b21\u53ef\u5206\u679060\u591a\u79cd\u6c28\u57fa\u9178\u548c\u9170\u57fa\u8089\u78b1\uff0c\u7b5b\u67e540\u4f59\u79cd\u65b0\u751f\u513f\u9057\u4f20\u4ee3\u8c22\u75c5\uff1b\u5728\u8425\u517b\u7d20\u68c0\u6d4b\u4e2d\u4e00\u6b21\u53ef\u5206\u679020\u79cd\u6c28\u57fa\u9178\u300120\u79cd\u8102\u80aa\u9178\u300110\u4f59\u79cd\u5fae\u91cf\u5143\u7d20\u62165\u79cd\u8102\u6eb6\u6027\u7ef4\u751f\u7d20\uff0c\u6709\u6548\u63d0\u9ad8\u4e86\u68c0\u6d4b\u901a\u91cf\u3001\u51cf\u5c11\u4e86\u6837\u54c1\u7528\u91cf\uff0c\u5e76\u63d0\u4f9b\u4e86\u4e30\u5bcc\u7684\u68c0\u6d4b\u4fe1\u606f\uff1b\u5728\u6bd2\u7406\u5b66\u68c0\u6d4b\u4e2d\u4e00\u6b21\u53ef\u68c0\u6d4b\u5c3f\u6db2\u4e2d19\u79cd\u836f\u7269\uff0c\u5b9e\u73b0\u4e86\u9ad8\u901a\u91cf\u3001\u5feb\u901f\u9ad8\u6548\u7684\u836f\u7269\u7b5b\u67e5\u6280\u672f[7]<\/sup>\u3002<\/span><\/p>\n


<\/p>\n

\u5728\u4e34\u5e8a\u751f\u5316\u68c0\u9a8c\u9886\u57df\uff0c\u8d28\u8c31\u6280\u672f\u76f8\u6bd4\u4e8e\u4f20\u7edf\u65b9\u6cd5\u7684\u4f18\u52bf\u8f83\u4e3a\u7a81\u51fa\uff0c\u4f46\u968f\u7740\u6280\u672f\u7684\u6df1\u5165\u5e94\u7528\u4e0e\u7ecf\u9a8c\u7684\u79ef\u7d2f\uff0c\u6280\u672f\u5e94\u7528\u7684\u7f3a\u70b9\u4e5f\u9010\u6b65\u51f8\u663e\u51fa\u6765\uff0c\u5305\u62ec\u8d28\u8c31\u6280\u672f\u5e94\u7528\u7684\u9677\u9631\u95ee\u9898\u3001\u5b9e\u9a8c\u5ba4\u65e5\u5e38\u8fd0\u884c\u8fc7\u7a0b\u4e2d\u7684\u7ba1\u7406\u95ee\u9898\u4ee5\u53ca\u76f8\u5173\u653f\u7b56\u6cd5\u89c4\u95ee\u9898\u7b49\uff0c\u4e3b\u8981\u4f53\u73b0\u5728\uff1a<\/span><\/p>\n


<\/p>\n

(1) <\/span><\/strong>\u8d28\u8c31\u6280\u672f\u5728\u5206\u6790\u57fa\u8d28\u590d\u6742\u7684\u751f\u7269\u6837\u672c\u65f6\uff0c\u68c0\u6d4b\u7ed3\u679c\u6613\u53d7\u5230\u57fa\u8d28\u6548\u5e94\u3001\u7ed3\u6784\u7c7b\u4f3c\u7269\u5e72\u6270\u4ee5\u53ca\u8d28\u8c31\u4fe1\u53f7\u4ea7\u751f\u7684\u4e0d\u7a33\u5b9a\u6240\u5e26\u6765\u7684\u5e72\u6270\u5f71\u54cd\uff1b\u5bf9\u8fd9\u4e9b\u95ee\u9898\u8ba4\u8bc6\u548c\u9884\u9632\u4e0d\u5f53\uff0c\u5219\u8d28\u8c31\u7684\u68c0\u6d4b\u7ed3\u679c\u5c06\u5b58\u5728\u8f83\u5927\u7684\u9519\u8bef\u98ce\u9669\uff1b<\/span><\/p>\n


<\/p>\n

(2) <\/span><\/strong>\u8d28\u8c31\u6280\u672f\u76f8\u6bd4\u4e8e\u514d\u75ab\u5b66\u65b9\u6cd5\u548c\u5316\u5b66\u53d1\u5149\u6cd5\uff0c\u68c0\u6d4b\u7684\u81ea\u52a8\u5316\u7a0b\u5ea6\u8f83\u4f4e\uff0c\u5bf9\u4eba\u5458\u4f9d\u8d56\u6027\u8f83\u5927\uff1b\u540c\u65f6\u5404\u5382\u5bb6\u4eea\u5668\u7cfb\u7edf\u8fd8\u672a\u5b9e\u73b0\u4e0e\u4e34\u5e8a\u5b9e\u9a8c\u5ba4\u4fe1\u606f\u7ba1\u7406\u7cfb\u7edf (LIS) \u7684\u63a5\u53e3\u53cc\u5411\u5bf9\u63a5\uff0c\u5728\u6570\u636e\u5904\u7406\u548c\u62a5\u544a\u53d1\u653e\u73af\u8282\uff0c\u4ecd\u672a\u5b9e\u73b0\u81ea\u52a8\u5316\uff1b<\/span><\/p>\n


<\/p>\n

(3) <\/span><\/strong>\u5bf9\u4e8e\u8d28\u8c31\u6280\u672f\u5e94\u7528\u8f83\u6210\u719f\u7684\u9879\u76ee\uff0c\u68c0\u6d4b\u6570\u636e\u4ecd\u7f3a\u4e4f\u7edf\u4e00\u7684\u5e94\u7528\u6807\u51c6[4]<\/sup>\uff1b<\/span><\/p>\n


<\/p>\n

(4) <\/span><\/strong>\u8d28\u8c31\u6280\u672f\u68c0\u6d4b\u65b9\u6cd5\u6240\u9700\u7684\u6807\u51c6\u7269\u8d28\u3001\u8bd5\u5242\u548c\u8017\u6750\u7b49\uff0c\u76ee\u524d\u4e3b\u8981\u4f9d\u8d56\u4e8e\u8fdb\u53e3\uff0c\u8f83\u591a\u7684\u68c0\u6d4b\u9879\u76ee\u53d7\u9650\u4e8e\u8fd9\u4e9b\u56e0\u7d20\u800c\u5f00\u5c55\u53d7\u963b\uff1b<\/span><\/p>\n


<\/p>\n

(5) <\/span><\/strong>\u76ee\u524d\u8d28\u8c31\u5b9e\u9a8c\u5ba4\u7684\u65b9\u6cd5\u57fa\u672c\u4e3a\u81ea\u5efa\u65b9\u6cd5\uff0c\u6807\u51c6\u5316\u548c\u89c4\u8303\u5316\u8f83\u4e3a\u8584\u5f31\u3002\u7f8e\u56fd\u4e34\u5e8a\u5b9e\u9a8c\u5ba4\u6807\u51c6\u5316\u534f\u4f1a\u5df2\u53d1\u5e03\u4e86\u4e34\u5e8a\u8d28\u8c31\u7684\u4f7f\u7528\u6307\u5357[8]<\/sup>\uff0c\u4e2d\u534e\u533b\u5b66\u4f1a\u68c0\u9a8c\u533b\u5b66\u5206\u4f1a\u3001\u536b\u751f\u8ba1\u751f\u59d4\u4e34\u5e8a\u68c0\u9a8c\u4e2d\u5fc3\u548c\u300a\u4e2d\u534e\u68c0\u9a8c\u533b\u5b66\u6742\u5fd7\u300b\u7f16\u8f91\u90e8\u4e5f\u4e8e2017\u5e7410\u6708\u4efd\u5171\u540c\u53d1\u5e03\u4e86\u300a\u6db2\u76f8\u8272\u8c31-\u8d28\u8c31\u4e34\u5e8a\u5e94\u7528\u5efa\u8bae\u300b[9]<\/sup>\uff0c\u8fd9\u4e9b\u90fd\u4e3a\u8d28\u8c31\u6280\u672f\u4e34\u5e8a\u68c0\u6d4b\u5de5\u4f5c\u63d0\u4f9b\u826f\u597d\u4e86\u7684\u6307\u5bfc\u548c\u53c2\u8003\uff1b<\/span><\/p>\n


<\/p>\n

(6) <\/span><\/strong>\u7531\u4e8e\u8d28\u8c31\u6280\u672f\u8f83\u4e3a\u590d\u6742\uff0c\u4eea\u5668\u6784\u6210\u591a\u6837\u5316\uff0c\u5728\u5b9e\u9645\u7684\u5e94\u7528\u8fc7\u7a0b\u4e2d\uff0c\u9700\u8981\u6709\u7ecf\u9a8c\u7684\u4e13\u4e1a\u6280\u672f\u4eba\u624d\u8fdb\u884c\u89c4\u8303\u7684\u4f7f\u7528\u64cd\u4f5c\uff0c\u4f46\u76ee\u524d\u56fd\u5185\u76f8\u5173\u7684\u6280\u672f\u4eba\u624d\u532e\u4e4f\uff1b\u8d28\u8c31\u5b9e\u9a8c\u5ba4\u7684\u4eea\u5668\u8bbe\u5907\u6602\u8d35\uff0c\u5bf9\u4e8e\u5b89\u88c5\u6761\u4ef6\u6709\u7279\u6b8a\u8981\u6c42\uff0c\u5efa\u8bbe\u9700\u8981\u6295\u5165\u5927\u91cf\u7684\u8d44\u91d1\uff1b\u8fd9\u4e9b\u4f7f\u5f97\u8d28\u8c31\u6280\u672f\u4e34\u5e8a\u5e94\u7528\u7684\u95e8\u69db\u8f83\u9ad8\uff0c\u4e00\u5b9a\u7a0b\u5ea6\u4e0a\u9650\u5236\u4e86\u6280\u672f\u7684\u5e94\u7528\uff1b<\/span><\/p>\n


<\/p>\n

(7) <\/span><\/strong>\u5728\u65e5\u5e38\u8fd0\u8425\u8fc7\u7a0b\u4e2d\u4eea\u5668\u7684\u7ef4\u4fee\u670d\u52a1\u6210\u672c\u8f83\u9ad8\uff0c\u7ef4\u4fee\u5468\u671f\u8f83\u957f\uff0c\u7ef4\u4fee\u7684\u53ca\u65f6\u6027\u4e5f\u5b58\u5728\u4e0d\u80fd\u6ee1\u8db3\u4e34\u5e8a\u68c0\u6d4b\u7684\u62a5\u544a\u5468\u671f\u56fa\u5b9a\u6027\u7684\u8981\u6c42\uff1b

<\/span><\/p>\n

(8) <\/span><\/strong>\u56fd\u5185\u5bf9\u4e8e\u8d28\u8c31\u6280\u672f\u5728\u4e34\u5e8a\u7684\u5e94\u7528\u76d1\u7ba1\u8fd8\u4e0d\u6210\u719f\uff0c\u76f8\u5173\u7684\u68c0\u6d4b\u9879\u76ee\u5728\u4e34\u5e8a\u4e0a\u65e0\u6536\u8d39\u6807\u51c6\uff0c\u4e5f\u5728\u4e00\u5b9a\u7a0b\u5ea6\u4e0a\u9650\u5236\u4e86\u6280\u672f\u7684\u5e94\u7528\u666e\u53ca\u3002<\/span><\/p>\n


<\/p>\n

\u867d\u7136\u8d28\u8c31\u6280\u672f\u7684\u5e94\u7528\u4ecd\u5b58\u5728\u8f83\u591a\u7f3a\u9677\uff0c\u4f46\u968f\u7740\u6280\u672f\u7684\u9769\u65b0\u4e0e\u53d1\u5c55\uff0c\u5e94\u7528\u76d1\u7ba1\u7684\u6210\u719f\uff0c\u5404\u9879\u74f6\u9888\u5c06\u88ab\u4e0d\u65ad\u7a81\u7834\uff0c\u672a\u6765\u968f\u7740\u8d28\u8c31\u4eea\u5668\u7684\u5404\u9879\u6027\u80fd\u7684\u63d0\u5347\uff1b\u524d\u5904\u7406\u81ea\u52a8\u5316\u7684\u5b9e\u73b0\uff1b\u68c0\u6d4b\u6570\u636e\u81ea\u52a8\u8f93\u51fa\u5e76\u5b9e\u73b0\u4e0e\u5b9e\u9a8c\u5ba4\u4fe1\u606f\u7cfb\u7edf\u7684\u53cc\u5411\u5bf9\u63a5\uff0c\u4ee5\u53ca\u7ed3\u679c\u62a5\u544a\u81ea\u52a8\u9884\u8b66\u529f\u80fd\u7684\u5b9e\u73b0\uff0c\u8d28\u8c31\u4eea\u6709\u671b\u50cf\u514d\u75ab\u5b66\u65b9\u6cd5\u548c\u5316\u5b66\u53d1\u5149\u6cd5\u4e00\u6837\uff0c\u6210\u4e3a\u4e34\u5e8a\u751f\u5316\u68c0\u9a8c\u4e2d\u81ea\u52a8\u5316\u3001\u667a\u80fd\u5316\u3001\u6613\u7528\u5316\u7684\u68c0\u6d4b\u5e73\u53f0\u3002<\/span><\/p>\n


<\/p>\n

2\u3001\u8d28\u8c31\u6280\u672f\u5728\u5fae\u751f\u7269\u68c0\u9a8c\u4e2d\u7684\u5e94\u7528<\/strong><\/span><\/p>\n

\u8fd1\u5e74\u6765\uff0cMALDI-TOF\u6280\u672f\u5df2\u6210\u529f\u5e94\u7528\u4e8e\u5fae\u751f\u7269\u7684\u9274\u5b9a\u53ca\u5206\u578b\uff0c\u5e76\u9010\u6e10\u6210\u4e3a\u5fae\u751f\u7269\u9274\u5b9a\u7684\u4e3b\u6d41\u6280\u672f\uff0c\u53ef\u5feb\u901f\u68c0\u6d4b\u548c\u9274\u5b9a\u9769\u5170\u9633\u6027\u83cc\u3001\u9769\u5170\u9634\u6027\u83cc\u3001\u538c\u6c27\u83cc\u3001\u5206\u679d\u6746\u83cc\u3001\u9175\u6bcd\u83cc\u548c\u4e1d\u72b6\u771f\u83cc\u7b49[6,10-14]<\/sup>\u3002\u76f8\u6bd4\u4e8e\u4f20\u7edf\u7684\u9769\u5170\u67d3\u8272\u3001\u83cc\u843d\u5f62\u6001\u3001\u8868\u578b\u9274\u5b9a\u53ca\u5206\u5b50\u751f\u7269\u5b66\u6280\u672f\uff0c MALDI-TOF\u6280\u672f\u5177\u6709\u5feb\u901f\u3001\u51c6\u786e\u3001\u7ecf\u6d4e\u3001\u9ad8\u901a\u91cf\u7b49\u4f18\u70b9\u3002MALDI-TOF\u662f\u57fa\u4e8e\u7ec6\u83cc\u8868\u9762\u86cb\u767d\u5206\u5b50\u68c0\u6d4b\u7684\u6280\u672f\uff0c\u901a\u8fc7\u6d4b\u5b9a\u672a\u77e5\u5fae\u751f\u7269\u81ea\u8eab\u72ec\u7279\u7684\u86cb\u767d\u8d28\u6307\u7eb9\u56fe\u8c31\u53ca\u7279\u5f81\u6027\u7684\u56fe\u8c31\u5cf0\uff0c\u5e76\u4e0e\u6570\u636e\u5e93\u4e2d\u53c2\u8003\u83cc\u682a\u7684\u86cb\u767d\u6307\u7eb9\u56fe\u8c31\u8fdb\u884c\u6bd4\u5bf9\uff0c\u4ece\u800c\u5b9e\u73b0\u83cc\u682a\u7684\u9274\u5b9a[11]<\/sup>\u3002<\/span><\/p>\n


<\/p>\n

\u8be5\u6280\u672f\u662f\u5c06\u5b8c\u6574\u7684\u5fae\u751f\u7269\u7ec6\u80de\u76f4\u63a5\u8fdb\u884c\u68c0\u6d4b\uff0c\u6837\u54c1\u5236\u5907\u7b80\u5355\uff0c\u68c0\u6d4b\u5468\u8f6c\u65f6\u95f4\u77ed\uff0c\u5728\u6570\u5206\u949f\u5185\u5c31\u53ef\u4ee5\u5f97\u5230\u4e00\u4e2a\u83cc\u79cd\u7684\u6d4b\u8bd5\u7ed3\u679c\uff0c\u4e14\u5206\u6790\u7528\u83cc\u91cf\u6781\u5c11\uff0c\u800c\u4f20\u7edf\u65b9\u6cd5\u5b8c\u6210\u5e38\u89c4\u7ec6\u83cc\u9274\u5b9a\u81f3\u5c11\u9700\u89818~18h\u6216\u66f4\u957f\u65f6\u95f4\u3002MALDI-TOF\u901a\u8fc7\u68c0\u6d4b\u7ec6\u83cc\u80de\u819c\u6210\u5206\u6216\u8868\u8fbe\u7684\u7279\u5f02\u86cb\u767d\u5bf9\u7ec6\u83cc\u8fdb\u884c\u79cd\u7fa4\u7684\u9274\u522b\uff0c\u654f\u611f\u6027\u548c\u51c6\u786e\u6027\u9ad8\uff0c\u53ef\u4ee5\u533a\u5206\u8868\u578b\u76f8\u4f3c\u6216\u76f8\u540c\u7684\u83cc\u682a\uff0c\u63d0\u4f9b\u5c5e\u3001\u79cd\u3001\u578b\u6c34\u5e73\u7684\u9274\u5b9a\uff0c\u5bf9\u4e34\u5e8a\u5e38\u89c1\u5206\u79bb\u83cc\u9274\u5b9a\u5230\u79cd\u6c34\u5e73\u7684\u51c6\u786e\u7387\u5f88\u9ad8\u3002\u4ee516S r RNA\u57fa\u56e0\u6d4b\u5e8f\u7ed3\u679c\u4e3a\u6807\u51c6\uff0c\u8d28\u8c31\u68c0\u6d4b\u7ed3\u679c\u51c6\u786e\u7387\u4e3a90.0%~95.0%[15]<\/sup>\uff0c\u4e0d\u4ec5\u53ef\u4ee5\u8bc6\u522b\u75c5\u539f\u83cc\uff0c\u800c\u4e14\u6709\u52a9\u4e8e\u53d1\u73b0\u65b0\u7684\u75c5\u539f\u83cc\u3002\u6b64\u5916\uff0c\u8d28\u8c31\u6280\u672f\u8fd8\u7528\u4e8e\u75c5\u539f\u4f53\u7684\u836f\u7269\u654f\u611f\u6027\u68c0\u6d4b\uff0c\u5e38\u89c4\u7684\u836f\u7269\u654f\u611f\u6027\u5b9e\u9a8c\u65b9\u6cd5\u6bd4\u8f83\u8d39\u65f6\uff0c\u5c40\u9650\u4e8e\u5c11\u6570\u7ec6\u83cc\uff0cMALDI-TOF\u901a\u8fc7\u6bd4\u5bf9\u8010\u836f\u83cc\u682a\u548c\u836f\u7269\u654f\u611f\u83cc\u682a\u95f4\u7684\u7279\u5f81\u6027\u86cb\u767d\u548c\u56fe\u8c31\u5cf0\u53ca\u68c0\u6d4b\u8010\u836f\u83cc\u682a\u4e0e\u6297\u751f\u7d20\u5171\u57f9\u517b\u540e\u7684\u5206\u89e3\u4ea7\u7269\uff0c\u53ef\u4ee5\u5206\u6790\u51e0\u4e4e\u6240\u6709\u7684\u8010\u836f\u673a\u5236\u3002<\/span><\/p>\n


<\/p>\n

\u7814\u7a76\u8868\u660e\uff0c\u76f8\u6bd4\u4e8e\u6807\u51c6\u7684\u5fae\u751f\u7269\u57f9\u517b\u6280\u672f\uff0c\u8d28\u8c31\u6280\u672f\u53ef\u964d\u4f4e\u7ea650%\u7684\u8bd5\u5242\u6210\u672c\u548c\u52b3\u52a8\u529b\u6210\u672c[16]<\/sup>\u3002\u4f46\u662f\uff0cMALDI-TOF\u4f5c\u4e3a\u4e00\u9879\u65b0\u5174\u6280\u672f\uff0c\u5728\u5fae\u751f\u7269\u9274\u5b9a\u65b9\u9762\u4e5f\u5b58\u5728\u7740\u4e00\u5b9a\u7684\u5c40\u9650\u6027\u3002\u5982\u5bf9\u4e8e\u5177\u6709\u7279\u6b8a\u7ed3\u6784\u7684\u83cc\u79cd\u548c\u56fe\u8c31\u6781\u4e3a\u76f8\u4f3c\u7684\u83cc\u79cd\u7684\u9274\u5b9a\u533a\u5206\u5b58\u5728\u4e00\u5b9a\u7684\u96be\u5ea6\u3001\u5bf9\u4e8e\u4e00\u4e9b\u7f55\u89c1\u83cc\u79cd\u6216\u65b0\u578b\u7ec6\u83cc\u9274\u5b9a\u56f0\u96be\u3001\u5bf9\u8840\u57f9\u517b\u6837\u672c\u4e2d\u7684\u6df7\u5408\u83cc\u79cd\u96be\u4ee5\u51c6\u786e\u9274\u522b\u7b49\uff0c\u539f\u56e0\u662f\u8d28\u8c31\u6570\u636e\u5e93\u4e2d\u6807\u51c6\u83cc\u682a\u7684\u56fe\u8c31\u6709\u9650\u3001\u8d28\u8c31\u5cf0\u7684\u6570\u636e\u4e0d\u5145\u5206\u4ee5\u53ca\u7ec6\u83cc\u5e93\u4e2d\u65e0\u8fd9\u4e9b\u83cc\u682a[17,18]<\/sup>\u3002<\/span><\/p>\n


<\/p>\n

\u968f\u7740\u4eea\u5668\u6280\u672f\u53c2\u6570\u3001\u8d28\u8c31\u6570\u636e\u5e93\u53ca\u5206\u6790\u8f6f\u4ef6\u7684\u4e0d\u65ad\u66f4\u65b0\u5b8c\u5584\uff0c\u6240\u6709\u7684\u5206\u79bb\u682a\u5c06\u88ab\u9010\u6b65\u7684\u660e\u786e\u9274\u5b9a\u51fa\u6765\u3002\u56e0\u6b64\uff0c\u968f\u7740\u8d28\u8c31\u6280\u672f\u5728\u4e34\u5e8a\u5fae\u751f\u7269\u5b9e\u9a8c\u5ba4\u7684\u5e94\u7528\u6570\u636e\u5e93\u8fdb\u4e00\u6b65\u5b8c\u5584\uff0cMALDI-TOF\u6280\u672f\u5fc5\u5c06\u5728\u5fae\u751f\u7269\u9274\u5b9a\u3001\u83cc\u79cd\u5206\u578b\u3001\u540c\u6e90\u5206\u6790\u3001\u8010\u836f\u76d1\u6d4b\u7b49\u591a\u65b9\u9762\u53d1\u6325\u51fa\u66f4\u5927\u4f5c\u7528\uff0c\u6709\u671b\u6210\u4e3a\u65b0\u4e00\u4ee3\u75c5\u539f\u5fae\u751f\u7269\u8bca\u65ad\u7684\u5e38\u89c4\u6280\u672f\u3002<\/span><\/p>\n


<\/p>\n

3\u3001\u8d28\u8c31\u6280\u672f\u5728\u6838\u9178\u68c0\u6d4b\u4e2d\u7684\u5e94\u7528<\/strong><\/span><\/p>\n

\u6838\u9178\u8d28\u8c31\u68c0\u6d4b\u6280\u672f\u662f\u5728MALDI-TOF\u539f\u7406\u7684\u57fa\u7840\u4e0a\uff0c\u7ed3\u5408\u5f15\u7269\u5ef6\u4f38\u5206\u6790\u6cd5\u548c\u78b1\u57fa\u7279\u5f02\u88c2\u89e3\u5206\u6790\u6cd5\uff0c\u9488\u5bf9\u53cc\u94feDNA\u7684\u7279\u6027\u8fdb\u884c\u4e86\u7279\u6b8a\u4f18\u5316\uff0c\u4f7f\u6837\u54c1\u5728\u7535\u79bb\u8fc7\u7a0b\u4e2d\u4e0d\u4ea7\u751f\u6216\u4ea7\u751f\u8f83\u5c11\u7684\u788e\u7247\u79bb\u5b50\uff0c\u53ef\u7528\u4e8e\u68c0\u6d4b\u6838\u9178\u7684\u5206\u5b50\u91cf\u548c\u7814\u7a76\u57fa\u56e0\u7ec4\u5355\u6838\u82f7\u9178\u591a\u6001\u6027 (single nucleotide polymorphism, SNP) \uff0c\u662f\u8fd1\u5e74\u6765\u5e94\u7528\u4e8e\u4e34\u5e8a\u6838\u9178\u68c0\u6d4b\u7684\u65b0\u578b\u8f6f\u7535\u79bb\u751f\u7269\u8d28\u8c31[19]<\/sup>\u3002\u76f8\u6bd4\u4e8e\u4ee5\u51dd\u80f6\u7535\u6cf3\u4e3a\u57fa\u7840\u7684\u6d4b\u5e8f\u6cd5\uff0c\u8d28\u8c31\u6280\u672f\u5177\u6709\u5206\u8fa8\u7387\u9ad8\u3001\u5206\u79bb\u901f\u5ea6\u5feb\u3001\u6742\u8d28\u5e72\u6270\u5c11\u7684\u4f18\u70b9\uff0c\u88ab\u5e7f\u6cdb\u5e94\u7528\u4e8e\u6838\u9178\u6d4b\u5e8f\u3001\u6838\u9178\u6307\u7eb9\u56fe\u8c31\u3001\u6838\u9178SNP\u5206\u6790\u7b49[20]<\/sup>\u3002<\/span><\/p>\n


<\/p>\n

SNP\u662f\u6307\u57fa\u56e0\u7ec4DNA\u5e8f\u5217\u4e0a\u67d0\u4e2a\u4f4d\u7f6e\u5355\u4e2a\u6838\u82f7\u9178\u78b1\u57fa\u7684\u5dee\u5f02\uff0c\u5373\u57fa\u56e0\u4f4d\u70b9\u7684\u7a81\u53d8\uff0c\u5728\u4eba\u7fa4\u4e2d\u7684\u53d1\u751f\u9891\u7387\u5927\u4e8e1%\uff0c\u662f\u51b3\u5b9a\u4e2a\u4f53\u75be\u75c5\u6613\u611f\u6027\u548c\u836f\u7269\u53cd\u5e94\u6027\u5dee\u5f02\u7684\u91cd\u8981\u56e0\u7d20\uff0c\u901a\u8fc7\u5206\u6790\u7a81\u53d8\u7684\u4f4d\u70b9\uff0c\u53ef\u9884\u6d4b\u75be\u75c5\uff0c\u5e76\u63d0\u4f9b\u8bca\u65ad\u610f\u89c1\u548c\u6307\u5bfc\u7528\u836f\u3002MALDI-TOF\u5206\u6790\u68c0\u6d4bSNP\u662f\u6839\u636e\u4e0d\u540c\u7684\u5206\u5b50\u91cf\u5c06\u7b49\u4f4d\u57fa\u56e0\u6392\u5e8f\uff0c\u533a\u5206\u548c\u9274\u522b\u76f8\u5bf9\u5206\u5b50\u91cf\u8fbe7000\u5de6\u53f3 (\u542b20\u591a\u4e2a\u78b1\u57fa) \u3001\u4ec5\u5b58\u57281\u4e2a\u78b1\u57fa\u5dee\u522b\u7684\u4e0d\u540cDNA\uff0c\u53ef\u4ee5\u7cbe\u51c6\u5730\u5206\u8fa8\u5230\u78b1\u57fa\u79cd\u7c7b\u3002<\/span><\/p>\n


<\/p>\n

\u836f\u7269\u4ee3\u8c22\u9176\u9057\u4f20\u591a\u6001\u6027\u662f\u4ea7\u751f\u836f\u7269\u6bd2\u526f\u4f5c\u7528\u3001\u964d\u4f4e\u6216\u4e27\u5931\u836f\u7269\u7597\u6548\u7684\u4e3b\u8981\u539f\u56e0\u4e4b\u4e00\uff0c\u901a\u8fc7\u68c0\u6d4b\u836f\u7269\u4ee3\u8c22\u9176\u7684\u57fa\u56e0\u578b\u53ef\u5bf9\u4e34\u5e8a\u7528\u836f\u65b9\u6848\u8fdb\u884c\u6307\u5bfc\u548c\u8c03\u6574\uff0c\u4e3a\u4e34\u5e8a\u4e2a\u4f53\u5316\u7528\u836f\u63d0\u4f9b\u4f9d\u636e\u3002\u4ee5\u5f80\u68c0\u6d4b\u836f\u7269\u4ee3\u8c22\u9176\u57fa\u56e0\u591a\u6001\u6027\u901a\u5e38\u91c7\u7528\u5316\u5b66\u6cd5\uff0c\u4f9d\u8d56\u4e8e\u6838\u82f7\u9178\u7684\u4e92\u8865\u6027\u5bf9\u6838\u9178\u5e8f\u5217\u8fdb\u884c\u5206\u6790\uff0c\u5bf9\u4e8e\u5e8f\u5217\u7684\u957f\u5ea6\u3001\u590d\u6742\u6027\u3001\u53cd\u5e94\u6761\u4ef6\u7b49\u90fd\u5177\u6709\u8f83\u9ad8\u7684\u8981\u6c42\uff0c\u5bb9\u6613\u53d7\u5230\u4e0d\u540c\u7a0b\u5ea6\u7684\u5316\u5b66\u56e0\u7d20\u5e72\u6270\uff0c\u5bfc\u81f4\u68c0\u6d4b\u7ed3\u679c\u51fa\u73b0\u504f\u5dee\u3002\u82e5\u80fd\u5c06\u5316\u5b66\u548c\u7269\u7406\u65b9\u6cd5\u7ed3\u5408\u8d77\u6765\u5bf9\u836f\u7269\u4ee3\u8c22\u9176\u57fa\u56e0\u8fdb\u884c\u68c0\u6d4b\uff0c\u5c06\u6781\u5927\u63d0\u9ad8\u68c0\u6d4b\u7ed3\u679c\u7684\u51c6\u786e\u6027\u3002<\/span><\/p>\n


<\/p>\n

MALDI-TOF\u662f\u836f\u7269\u4ee3\u8c22\u9176\u57fa\u56e0\u591a\u6001\u6027\u7684\u65b0\u578b\u68c0\u6d4b\u65b9\u6cd5\uff0c\u5176\u6839\u636e\u6838\u82f7\u9178\u5206\u5b50\u88ab\u7535\u79bb\u540e\u5728\u771f\u7a7a\u7ba1\u4e2d\u7684\u98de\u884c\u65f6\u95f4\u6765\u786e\u5b9a\u5176\u5206\u5b50\u91cf\u5927\u5c0f\uff0c\u6700\u7ec8\u786e\u5b9a\u6838\u82f7\u9178\u5e8f\u5217\uff0c\u68c0\u6d4b\u7ed3\u679c\u4ec5\u4ec5\u4f9d\u8d56\u4e8e\u6838\u9178\u5206\u5b50\u91cf\u3002\u7ecf\u8fc7\u9a8c\u8bc1\u6bd4\u8f83\uff0cMALDI-TOF\u68c0\u6d4b\u7ed3\u679c\u4e0eSanger\u6d4b\u5e8f\u7684\u7ed3\u679c\u7b26\u5408\u7387\u4e3a100%[21,22]<\/sup>\u3002\u4f20\u7edf\u7684Sanger\u6d4b\u5e8f\u65b9\u6cd5\u867d\u7136\u662f\u5e8f\u5217\u6d4b\u5b9a\u7684\u91d1\u6807\u51c6\uff0c\u4f46\u5176\u64cd\u4f5c\u6b65\u9aa4\u7e41\u7410\u8d39\u65f6\u548c\u8bd5\u5242\u6210\u672c\u9ad8\u7b49\u9650\u5236\u4e86\u5176\u4e34\u5e8a\u5e94\u7528\u3002MALDI-TOF\u53ef\u901a\u8fc7\u4e00\u6b21\u5b9e\u9a8c\u68c0\u6d4b\u591a\u4e2a\u6807\u672c\u7684\u591a\u4e2a\u7a81\u53d8\uff0c\u5b9e\u73b0\u57fa\u56e0\u578b\u7684\u9ad8\u901a\u91cf\u3001\u5feb\u901f\u68c0\u6d4b\uff0c\u4e3a\u4e2a\u4f53\u5316\u7528\u836f\u63d0\u4f9b\u66f4\u52a0\u591a\u6837\u5316\u7684\u68c0\u6d4b\u624b\u6bb5\u3002<\/span><\/p>\n


<\/p>\n

4\u3001\u8d28\u8c31\u6280\u672f\u5728\u86cb\u767d\u8d28\u7ec4\u5b66\u4e2d\u7684\u5e94\u7528<\/span><\/strong><\/span><\/p>\n

\u8d28\u8c31\u6280\u672f\u53ef\u68c0\u6d4b\u86cb\u767d\u8d28\u7684\u6c28\u57fa\u9178\u7ec4\u6210\u3001\u5206\u5b50\u91cf\u3001\u591a\u80bd\u6216\u4e8c\u786b\u952e\u7684\u6570\u76ee\u4e0e\u4f4d\u7f6e\u53ca\u86cb\u767d\u8d28\u7684\u7a7a\u95f4\u6784\u8c61\u7b49\uff0c\u4ece\u800c\u5b9e\u73b0\u672a\u77e5\u80bd\u6bb5\u7684\u7b5b\u9009\u3001\u6d4b\u5e8f\u3001\u80bd\u6307\u7eb9\u56fe\u8c31\u3001\u86cb\u767d\u8d28\u8868\u8fbe\u8c31\u3001\u86cb\u767d\u8d28\u7ffb\u8bd1\u540e\u4fee\u9970\u8c31\u3001\u5168\u86cb\u767d\u5b8c\u6574\u65e0\u635f\u5206\u6790\u7b49\u3002\u8d28\u8c31\u591a\u6837\u5316\u7684\u524d\u7aef\u8fde\u63a5\u65b9\u5f0f\u6781\u5927\u5730\u4fc3\u8fdb\u4e86\u7814\u7a76\u8005\u5bf9\u57fa\u7840\u86cb\u767d\u79d1\u5b66\u9886\u57df\u7684\u8ba4\u8bc6\uff0c\u4f46\u5c06\u8fd9\u4e9b\u8ba4\u8bc6\u8f6c\u53d8\u4e3a\u5bf9\u4e34\u5e8a\u5b9e\u8df5\u7684\u6709\u6548\u4fe1\u606f\u5219\u6709\u76f8\u5f53\u5927\u7684\u96be\u5ea6\u3002\u5230\u76ee\u524d\u4e3a\u6b62\uff0c\u57fa\u4e8e\u8d28\u8c31\u6280\u672f\u7684\u5c06\u86cb\u767d\u7ec4\u5b66\u591a\u6837\u6027\u7684\u86cb\u767d\u548c\u591a\u80bd\u6807\u5fd7\u7269\uff0c \u6210\u529f\u5e94\u7528\u4e8e\u4e34\u5e8a\u68c0\u6d4b\u7684\u6848\u4f8b\u5e76\u4e0d\u591a\u89c1[22]<\/sup>\u3002<\/span><\/p>\n


<\/p>\n

\u76f8\u53cd\uff0c\u5bf9\u4e8e\u5df2\u77e5\u7684\u3001\u786e\u5b9a\u7684\u591a\u80bd\u548c\u86cb\u767d\u6807\u5fd7\u7269\u5373\u76ee\u6807\u86cb\u767d\u7ec4\u5b66\uff0c\u8d28\u8c31\u6280\u672f\u5f97\u5230\u4e86\u8f83\u597d\u7684\u5e94\u7528\u3002\u76ee\u524d\uff0c\u5df2\u7ecf\u6709\u4e00\u4e9b\u5173\u4e8eLC-MS\/MS\u7528\u4e8e\u4e34\u5e8a\u76ee\u6807\u591a\u80bd\u548c\u86cb\u767d\u5206\u6790\u7684\u6587\u7ae0\u53d1\u8868\uff0c\u5982\u7532\u72b6\u817a\u7403\u86cb\u767d (Tg) \u548c\u6dc0\u7c89\u6837\u86cb\u767d\u7684\u9274\u5b9a\u4e0e\u5b9a\u91cf\u5206\u6790\u7b49[23-25]<\/sup>\u3002\u8d28\u8c31\u6280\u672f\u5728\u8fd9\u4e00\u9886\u57df\u7684\u5e94\u7528\uff0c\u5728\u5f88\u591a\u60c5\u51b5\u4e0b\u5747\u53ef\u4e3a\u4e34\u5e8a\u63d0\u4f9b\u6709\u4ef7\u503c\u7684\u4fe1\u606f[21]<\/sup>\uff0c\u5982\u5bf9\u67d0\u4e00\u5206\u6790\u7269\u7684\u514d\u75ab\u5b66\u65b9\u6cd5\u4e0d\u5b58\u5728\u65f6\uff1b\u5df2\u7ecf\u5b58\u5728\u7684\u514d\u75ab\u5b66\u65b9\u6cd5\u4e0d\u80fd\u7ed9\u51fa\u67d0\u4e9b\u4e34\u5e8a\u5173\u952e\u95ee\u9898\u7684\u7b54\u6848\u65f6\uff1b\u5df2\u7ecf\u5b58\u5728\u7684\u514d\u75ab\u5b66\u65b9\u6cd5\u5b58\u5728\u5e72\u6270\u65f6\uff1b\u67d0\u4e00\u5206\u6790\u7269\u5b58\u5728\u591a\u4e2a\u5f02\u6784\u4f53\u65f6\uff1b\u5bf9\u540c\u4e00\u5206\u6790\u7269\u7684\u68c0\u6d4b\uff0c\u4e0d\u540c\u7684\u68c0\u6d4b\u65b9\u6cd5\u95f4\u5b58\u5728\u8f83\u5927\u7684\u7ed3\u679c\u53d8\u5f02\u6027\u65f6\uff1b\u5df2\u7ecf\u5b58\u5728\u7684\u5206\u6790\u65b9\u6cd5\u6d41\u7a0b\u8f83\u4e3a\u590d\u6742\u65f6\uff0c\u8d28\u8c31\u6280\u672f\u5747\u53ef\u53d1\u6325\u76f8\u5e94\u4f5c\u7528\uff0c\u5f25\u8865\u514d\u75ab\u5b66\u65b9\u6cd5\u7684\u4e0d\u8db3\u3002<\/span><\/p>\n


<\/p>\n

\u8d28\u8c31\u6280\u672f\u5728\u533b\u5b66\u68c0\u9a8c\u9886\u57df\u4e2d\u5e94\u7528\u7684\u4e0b\u4e2a\u76ee\u6807\u548c\u6311\u6218\uff0c\u662f\u5982\u4f55\u5f25\u8865\u514d\u75ab\u5b66\u65b9\u6cd5\u5728\u86cb\u767d\u548c\u591a\u80bd\u68c0\u6d4b\u65b9\u9762\u7684\u5c40\u9650\u6027\u3002\u76f8\u4fe1\u968f\u7740\u6280\u672f\u7684\u53d1\u5c55\uff0c\u8fd9\u65b9\u9762\u7684\u7a81\u7834\u4f1a\u8d8a\u6765\u8d8a\u591a\uff0c\u4e3a\u4e34\u5e8a\u63d0\u4f9b\u66f4\u591a\u7684\u6709\u4ef7\u503c\u7684\u8d28\u8c31\u68c0\u6d4b\u6570\u636e\u3002<\/span><\/p>\n


<\/p>\n

\u3010\u53c2\u8003\u6587\u732e\u3011<\/span><\/p>\n

\n
\n
\n
\n

[1]\u6f58\u67cf\u7533.\u8fce\u63a5\u8d28\u8c31\u6280\u672f\u8fdb\u5165\u68c0\u9a8c\u533b\u5b66\u9886\u57df[J].\u4e2d\u534e\u68c0\u9a8c\u533b\u5b66\u6742\u5fd7, 2017, 40 (10) :733-736.<\/span><\/p>\n

[2] Jannetto PJ, Fitzgerald RL.Effective use of mass spectrometry in the clinical laboratory[J].Clin Chem, 2016, 62 (1) :92-98.<\/span><\/p>\n

[3] \u97e9\u4e3d\u4e54, \u5e84\u4fca\u534e, \u9ec4\u5baa\u7ae0.\u8d28\u8c31\u6280\u672f\u53ca\u5176\u5728\u4e34\u5e8a\u68c0\u9a8c\u4e2d\u7684\u5e94\u7528[J].\u68c0\u9a8c\u533b\u5b66, 2013, 28 (3) :252-256.<\/span><\/p>\n

[4] \u5f20\u81ea\u5f3a, \u674e\u5ca9.\u8d28\u8c31\u6280\u672f\u5728\u4e34\u5e8a\u751f\u5316\u68c0\u6d4b\u4e2d\u7684\u5e94\u7528[J].\u68c0\u9a8c\u533b\u5b66, 2015, 30 (5) :407-409.<\/span><\/p>\n

[5] \u674e\u6c34\u519b, \u738b\u601d\u5408.\u6db2\u76f8\u8272\u8c31-\u4e32\u8054\u8d28\u8c31\u6280\u672f\u7684\u4e34\u5e8a\u5e94\u7528\u8fdb\u5c55[J].\u4e34\u5e8a\u68c0\u9a8c\u6742\u5fd7, 2016, 34 (12) :881-884.<\/span><\/p>\n

[6] Vogeser M, Seger C.Quality management in clinical application of mass spectrometry measurement systems[J].Clin Biochem, 2016, 49 (13-14) :947-954.<\/span><\/p>\n

[7] \u738b\u601d\u5408, \u7a0b\u96c5\u5a77, \u8d75\u84d3\u84d3.\u4e34\u5e8a\u8272\u8c31\u8d28\u8c31\u68c0\u9a8c\u6280\u672f[M].\u5317\u4eac:\u4eba\u6c11\u536b\u751f\u51fa\u7248\u793e, 2017.<\/span><\/p>\n

[8] \u6c88\u7acb\u677e, \u9a6c\u598d\u6167.\u8d28\u8c31\u6280\u672f\u5728\u68c0\u9a8c\u533b\u5b66\u4e2d\u7684\u5e94\u7528\u73b0\u72b6\u548c\u524d\u666f[J].\u8bca\u65ad\u5b66\u7406\u8bba\u4e0e\u5b9e\u8df5, 2012, 11 (5) :536-538.<\/span><\/p>\n

[9] \u4e2d\u534e\u533b\u5b66\u4f1a\u68c0\u9a8c\u533b\u5b66\u5206\u4f1a, \u536b\u751f\u8ba1\u751f\u59d4\u4e34\u5e8a\u68c0\u9a8c\u4e2d\u5fc3.\u6db2\u76f8\u8272\u8c31-\u8d28\u8c31\u4e34\u5e8a\u5e94\u7528\u5efa\u8bae[J].\u4e2d\u534e\u68c0\u9a8c\u533b\u5b66\u6742\u5fd7, 2017, 40 (10) :770-779.<\/span><\/p>\n

[10] Van Veen SQ, Class ECJ, Kuijper EJ.High-throughput identification of bacteria and yeast by matrix-assisted laser desorption ionization time of flight mass spectrometry in conventional medical microbiology laboratories[J].J Clin Microbiol, 2010, 48 (3) :900-907.<\/span><\/p>\n

[11] Robert PR, Brosnikoff C, Turnbull L, et al.Multicenter evaluation of the vitek 2 anaerobe and Coryne bacterium identification card[J].J Clin Microbiol, 2008, 46 (8) :2646-2651.<\/span><\/p>\n

[12] Christopher DD, Susan MB.Emerging and future applications of Matrix-Assisted Laser Desorption Ionization Time-of-Flight (MALDITOF) Mass Spectrometry in the clinical microbiology laboratory[J].J Mol Diagn, 2016, 18 (6) :789-802.<\/span><\/p>\n

[13] Ovia\u00f1o M, Fern\u00e1ndez B, Fern\u00e1ndez A, et al.Rapid detection of enterobacteriaceae producing extended spectrum beta-Lactamases directly from positive blood cultures by matrix-assisted laser desorption ionization time-of-flight mass spectrometry[J].Clin Microbiol Infect, 2014, 20 (11) :1146-1157.<\/span><\/p>\n

[14] McMullen AR, Burnham CA.Be serious:posttraumatic endophthalmitis[J].Clin Chem, 2016, 62 (1) :37-39.<\/span><\/p>\n

[15] \u4e2d\u56fd\u4e34\u5e8a\u5fae\u751f\u7269\u8d28\u8c31\u5e94\u7528\u4e13\u5bb6\u5171\u8bc6\u4e13\u5bb6\u7ec4.\u4e2d\u56fd\u4e34\u5e8a\u5fae\u751f\u7269\u8d28\u8c31\u5e94\u7528\u4e13\u5bb6\u5171\u8bc6[J].\u4e2d\u534e\u533b\u9662\u611f\u67d3\u5b66\u6742\u5fd7, 2016, 26 (10) :I-IV.<\/span><\/p>\n

[16] Tan KE, Ellis BC, Lee R, et al.Prospective evaluation of a matrixassisted laser desorption ionization-time of flight mass spectrometry system in a hospital clinical microbiology laboratory for identification of bacteria and yeasts:a bench-by-bench study for assessing the impact on time to identification and cost-effectiveness[J].J Clin Microbiol, 2012, 50 (10) :3301-3308.<\/span><\/p>\n

[17] Dingle TC, Butler-Wu SM.MALDI-TOF mass spectrometry for microorganism identification[J].Clin Lab Med, 2013, 33 (3) :589-609.<\/span><\/p>\n

[18] Clark AE, Kaleta EJ, Arora A, et al.Matrix-assisted laser desorption ionization-time of flight mass spectrometry:a fundamental shift in the routine practice of clinical microbiology[J].Clin Microbiol Rev, 2013, 26 (3) :547-603.<\/span><\/p>\n

[19] Edwards JR, Ruparel H, Ju J.Mass-spectrometry DNA sequencing[J].Mutat Res, 2005, 573 (1-2) :3-12.<\/span><\/p>\n

[20] Sauer S, Reinhardt R, Lehrach H, et al.Single nucleotide polymorphisms:analysis by mass spectrometry[J].Nat Protoc, 2006, 1 (4) :1761-1771.<\/span><\/p>\n

[21] \u53f6\u963f\u91cc, \u5f20\u6d77\u71d5, \u7aa6\u4e9a\u73b2, \u7b49.\u57fa\u8d28\u8f85\u52a9\u6fc0\u5149\u89e3\u5438\u7535\u79bb\u98de\u884c\u65f6\u95f4\u8d28\u8c31\u6280\u672f\u68c0\u6d4b\u836f\u7269\u4ee3\u8c22\u9176\u57fa\u56e0\u591a\u6001\u6027\u5e73\u53f0\u7684\u5efa\u7acb[J].\u73b0\u4ee3\u68c0\u9a8c\u533b\u5b66\u6742\u5fd7, 2016, 31 (5) :30-33.<\/span><\/p>\n

[22] Grebe SKG, Singh RJ.LC-MS\/MS in the clinical laboratory-where to form here[J]?Clin Biochem Rev, 2011, 32 (1) :5-31.<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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