{"id":19107,"date":"2018-07-17T01:00:00","date_gmt":"2018-08-08T01:08:45","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/2018\/08\/08\/%e7%ba%b3%e7%b1%b3%e9%a2%97%e7%b2%92%e4%bc%a0%e9%80%92%e6%8a%80%e6%9c%af%e5%8a%a9%e5%8a%9bcrispr%e6%b2%bb%e7%96%97%e8%87%aa%e9%97%ad%e7%97%87\/"},"modified":"2018-08-08T09:08:47","modified_gmt":"2018-08-08T01:08:47","slug":"%e7%ba%b3%e7%b1%b3%e9%a2%97%e7%b2%92%e4%bc%a0%e9%80%92%e6%8a%80%e6%9c%af%e5%8a%a9%e5%8a%9bcrispr%e6%b2%bb%e7%96%97%e8%87%aa%e9%97%ad%e7%97%87","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/19107\/","title":{"rendered":"\u7eb3\u7c73\u9897\u7c92\u4f20\u9012\u6280\u672f\u52a9\u529bCRISPR\u6cbb\u7597\u81ea\u95ed\u75c7"},"content":{"rendered":"<div class=\"bpp-post-content\">\n<p style=\"margin-right: 16px;margin-left: 16px;max-width: 100%;min-height: 1em;line-height: 2em;text-indent: 2em;box-sizing: border-box !important;word-wrap: break-word !important;text-align: justify;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u52a0\u5dde\u5927\u5b66\u4f2f\u514b\u5229\u5206\u6821\u7684\u79d1\u7814\u4eba\u5458\u53d1\u660e\u4e86\u4e00\u79cd\u540d\u4e3aCRISPR-Gold\u7684\u6280\u672f\uff0c\u5176\u5229\u7528\u91d1\u7eb3\u7c73\u7c92\u5b50\u5c06\u5207\u5272DNA\u7684Cas9\u9176\u4f20\u9012\u5230\u5927\u8111\u4e2d\uff0c\u4f7f\u7814\u7a76\u4eba\u5458\u80fd\u591f\u7f16\u8f91\u795e\u7ecf\u9012\u8d28\u53d7\u4f53\u57fa\u56e0\uff0c\u5e76\u51cf\u5c11\u8106\u6027X\u7efc\u5408\u5f81\uff08FXS\uff09\u7684\u5938\u5f20\u6027\u91cd\u590d\u884c\u4e3a\u8fd9\u79cd\u53d1\u75c5\u75c7\u72b6\u7684\u53d1\u751f\u3002<\/span><\/p>\n<p style=\"margin-right: 16px;margin-left: 16px;max-width: 100%;min-height: 1em;text-indent: 2em;line-height: 2em;box-sizing: border-box !important;word-wrap: break-word !important;text-align: justify;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u5728San Antonio\u5065\u5eb7\u79d1\u5b66\u4e2d\u5fc3\u7684\u7814\u7a76\u8d1f\u8d23\u4eba\u517cTexas\u5927\u5b66\u7684\u7ec6\u80de\u4e0e\u7efc\u5408\u751f\u7406\u5b66\u52a9\u7406\u6559\u6388Hye Young Lee\u8868\u793a\uff0c\u76ee\u524d\u8fd8\u6ca1\u6709\u6cbb\u7597\u624b\u6bb5\u6765\u6cbb\u6108\u81ea\u95ed\u75c7\uff0c\u800c\u4e14\u8bb8\u591a\u9488\u5bf9\u9776\u5411\u86cb\u767d\u8d28\u7684\u5c0f\u5206\u5b50\u6cbb\u7597\u7684\u4e34\u5e8a\u8bd5\u9a8c\u90fd\u5931\u8d25\u4e86\u3002\u8be5\u7814\u7a76\u662f\u80fd\u591f\u7f16\u8f91\u5927\u8111\u4e2d\u81ea\u95ed\u75c7\u81f4\u75c5\u57fa\u56e0\u7684\u7b2c\u4e00\u4e2a\u6848\u4f8b\uff0c\u8fd9\u6781\u5927\u5730\u6539\u5584\u4e86\u5b9e\u9a8c\u4e2d\u5c0f\u9f20\u7684\u884c\u4e3a\u80fd\u529b\u3002<\/span><\/p>\n<p style=\"margin-right: 16px;margin-left: 16px;max-width: 100%;min-height: 1em;text-indent: 2em;line-height: 2em;box-sizing: border-box !important;word-wrap: break-word !important;text-align: justify;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u7814\u7a76\u4eba\u5458\u8ba4\u4e3a\uff0c\u4e0e\u5176\u5b83\u5c06Cas9\u690d\u5165\u4f53\u5185\u7684\u65b9\u6cd5\u76f8\u6bd4\uff08\u6bd4\u5982\u5229\u7528\u75c5\u6bd2\u4f20\u9012\uff09\uff0cCRISPR-Gold\u5177\u6709\u66f4\u591a\u4f18\u52bf\u3002<\/span><\/p>\n<p style=\"margin-right: 16px;margin-left: 16px;max-width: 100%;min-height: 1em;text-indent: 2em;line-height: 2em;box-sizing: border-box !important;word-wrap: break-word !important;text-align: justify;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">CRISPR-Gold\u7684\u53d1\u660e\u8005\uff0c\u52a0\u5dde\u5927\u5b66\u4f2f\u514b\u5229\u5206\u6821\u751f\u7269\u5de5\u7a0b\u5b66\u6559\u6388Niren Murthy\u8ba4\u4e3a\uff0c\u8fd9\u7bc7\u8bba\u6587\u771f\u6b63\u5f15\u4eba\u6ce8\u76ee\u7684\u662f\uff0cHye Young\u8bc1\u660e\u4e86\u5982\u679c\u5c06CRISPR-Gold\u6ce8\u5c04\u5230\u5927\u8111\u4e2d\uff0c\u5c31\u53ef\u4ee5\u5254\u9664\u81f4\u75c5\u57fa\u56e0\uff0c\u5e76\u4ea7\u751f\u6781\u5927\u7684\u884c\u4e3a\u80fd\u529b\u53d8\u5316\u3002\u8fd9\u662f\u6709\u53f2\u4ee5\u6765\u7b2c\u4e00\u6b21\u7531\u975e\u75c5\u6bd2\u65b9\u6cd5\u8fdb\u884c\u4f20\u9012\u7684\u6848\u4f8b\u3002<\/span><\/p>\n<p style=\"margin-right: 16px;margin-left: 16px;max-width: 100%;min-height: 1em;text-indent: 2em;line-height: 2em;box-sizing: border-box !important;word-wrap: break-word !important;text-align: justify;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u60a3\u6709\u81ea\u95ed\u75c7\u8c31\u7cfb\u969c\u788d\uff08ASD\uff09\u7684\u4eba\u901a\u5e38\u6709\u4e0e\u4ed6\u4eba\u96be\u4ee5\u4ea4\u5f80\u7684\u95ee\u9898\uff0c\u4ee5\u53ca\u8868\u73b0\u51fa\u5938\u5f20\u6027\u7684\u91cd\u590d\u884c\u4e3a\uff0c\u4f8b\u5982\u91cd\u590d\u7684\u6447\u6446\u548c\u62cd\u6253\u3002\u867d\u7136ASD\u5f80\u5f80\u6709\u591a\u79cd\u81f4\u75c5\u539f\u56e0\uff0c\u5305\u62ec\u591a\u79cd\u57fa\u56e0\u7684\u53d8\u5f02\uff0c\u4f46\u73b0\u5728\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u5148\u901a\u8fc7FXS\u8fd9\u6837\u7684\u5355\u57fa\u56e0\u75be\u75c5\u6765\u63a2\u7d22\u75c5\u56e0\u548c\u6f5c\u5728\u7684\u6cbb\u7597\u65b9\u6cd5\u3002\u5168\u4e16\u754c\u6709\u5927\u7ea61%\u7684\u513f\u7ae5\u60a3\u6709\u81ea\u95ed\u75c7\u8c31\u7cfb\u969c\u788d\uff0c\u4f46FXS\u5f88\u7f55\u89c1\uff0c\u5927\u7ea6\u6bcf4,000\u540d\u7537\u5b69\u548c6,000\u540d\u5973\u5b69\u4e2d\u4f1a\u6709\u4e00\u540d\u3002<\/span><\/p>\n<p style=\"margin-right: 16px;margin-left: 16px;max-width: 100%;min-height: 1em;text-indent: 2em;line-height: 2em;box-sizing: border-box !important;word-wrap: break-word !important;text-align: justify;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">CRISPR-Gold\u8fd9\u9879\u65b0\u7814\u7a76\u9996\u6b21\u8bc1\u660e\u4e86Cas9\u86cb\u767d\u53ef\u4ee5\u88ab\u8fd0\u9001\u5230\u5927\u8111\u4e2d\u4ee5\u6572\u9664\u57fa\u56e0\u5e76\u6709\u6cbb\u7597\u6548\u679c\u3002\u867d\u7136\u5176\u4ed6\u7814\u7a76\u4eba\u5458\u5df2\u7ecf\u901a\u8fc7\u50cf\u7528\u817a\u76f8\u5173\u75c5\u6bd2\uff08AAV\uff09\u5c06Cas9\u57fa\u56e0\u4f20\u9012\u5230\u795e\u7ecf\u5143\u4e2d\uff0c\u4f46\u7531\u4e8e\u8be5\u57fa\u56e0\u4e0d\u65ad\u751f\u4ea7Cas9\u9176\uff0c\u8fc7\u591a\u7684Cas9\u9176\u4f1a\u5bfc\u81f4\u5176\u5b83\u57fa\u56e0\u88ab\u6572\u9664\uff0c\u5f15\u8d77\u5176\u5b83\u65b9\u9762\u7684\u95ee\u9898\u3002\u76f8\u6bd4\u4e4b\u4e0b\uff0cCRISPR-Gold\u672c\u8eab\u643a\u5e26Cas9\u590d\u5408\u7269\uff08\u5305\u62ec\u63d0\u7eaf\u7684Cas9\u86cb\u767d\u548c\u5f15\u5bfcRNA\uff09\u76f4\u63a5\u8fdb\u5165\u7ec6\u80de\uff0c\u5728\u90a3\u91cc\u5b83\u53ea\u4f1a\u8fdb\u884c\u51e0\u6b21\u57fa\u56e0\u526a\u5207\uff0c\u968f\u540e\u5c31\u4f1a\u6d88\u5931\u3002<\/span><\/p>\n<p style=\"margin-right: 16px;margin-left: 16px;max-width: 100%;min-height: 1em;text-indent: 2em;line-height: 2em;box-sizing: border-box !important;word-wrap: break-word !important;text-align: justify;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u5982\u679c\u7528AAV\u75c5\u6bd2\u6ce8\u5c04CRISPR DNA\uff0c\u5c06\u4f1a\u65e0\u6cd5\u63a7\u5236Cas9\u86cb\u767d\u548c\u5f15\u5bfcRNA\u7684\u5242\u91cf\uff0c\u5e76\u4f1a\u5bfc\u81f4\u6f5c\u5728\u7684\u95ee\u9898\u3002Lee\u8ba4\u4e3a\uff0cCRISPR-Gold\u65b9\u6cd5\u975e\u5e38\u6709\u6548\uff0c\u56e0\u4e3a\u79d1\u5b66\u5bb6\u53ef\u4ee5\u63a7\u5236\u5e0c\u671b\u6ce8\u5c04\u7684\u5242\u91cf\uff0c\u4ece\u800c\u53ef\u80fd\u6700\u5c0f\u5316CRISPR\u7684\u526f\u4f5c\u7528\uff0c\u4f8b\u5982\u8131\u9776\u6548\u5e94\u3002<\/span><\/p>\n<p style=\"margin-right: 16px;margin-left: 16px;max-width: 100%;min-height: 1em;text-indent: 2em;line-height: 2em;box-sizing: border-box !important;word-wrap: break-word !important;text-align: justify;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">Murthy\u8ba4\u4e3a\uff0c\u8fd9\u9879\u6280\u672f\u4e3a\u6cbb\u7597\u963f\u7247\u7c7b\u836f\u7269\u6210\u763e\uff0c\u795e\u7ecf\u6027\u75bc\u75db\uff0c\u7cbe\u795e\u5206\u88c2\u75c7\u548c\u766b\u75eb\u53d1\u4f5c\u7b49\u75be\u75c5\u6253\u5f00\u4e86\u5927\u95e8\u3002<\/span><\/p>\n<p style=\"margin-right: 16px;margin-left: 16px;max-width: 100%;min-height: 1em;text-indent: 2em;line-height: 2em;box-sizing: border-box !important;word-wrap: break-word !important;text-align: justify;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u8be5\u7814\u7a76\u53ef\u4ee5\u963b\u6b62\u8fc7\u5ea6\u5174\u594b\u7684\u5927\u8111\u3002\u5728\u60a3\u6709FXS\u7684\u5c0f\u9f20\u5b9e\u9a8c\u4e2d\uff0c\u7814\u7a76\u4eba\u5458\u5c06\u8f7d\u6709Cas9\u590d\u5408\u7269\u7684CRISPR-Gold\u6ce8\u5165\u5927\u8111\u5185\u7684\u7eb9\u72b6\u4f53\u3002\u7eb9\u72b6\u4f53\u662f\u5927\u8111\u63a7\u5236\u4eba\u7c7b\u4e60\u6027\u7684\u4e00\u5757\u533a\u57df\uff0c\u548cASD\u7684\u60a3\u8005\u4e00\u6837\uff0c\u4e00\u4e9b\u5e38\u89c1\u7684\u91cd\u590d\u884c\u4e3a\u4e5f\u88ab\u7eb9\u72b6\u4f53\u63a7\u5236\u7740\u3002Cas9\u4ee5\u5174\u594b\u6027\u53d7\u4f53\uff0c\u5373\u4ee3\u8c22\u578b\u8c37\u6c28\u9178\u53d7\u4f535\uff08mGluR5\uff09\u4e3a\u9776\u5411\u3002mGluR\u5728\u795e\u7ecf\u5143\u4e4b\u95f4\u7684\u901a\u8baf\u8fc7\u7a0b\u4e2d\u8d77\u5230\u4f5c\u7528\uff0c\u4f46\u662fFXS\u60a3\u8005\u7684mGluR5\u663e\u793a\u4e3a\u5f02\u5e38\u3002\u901a\u8fc7\u4f7fmGluR5\u57fa\u56e0\u5931\u53bb\u529f\u80fd\uff0c\u7814\u7a76\u4eba\u5458\u80fd\u591f\u6291\u5236\u7ec6\u80de\u4e4b\u95f4\u5938\u5927\u7684\u4fe1\u53f7\u4f20\u5bfc\u5e76\u51cf\u5c11\u60a3\u8005\u7684\u5938\u5f20\u6027\u91cd\u590d\u884c\u4e3a\u3002<\/span><\/p>\n<p style=\"margin-right: 16px;margin-left: 16px;max-width: 100%;min-height: 1em;text-indent: 2em;line-height: 2em;box-sizing: border-box !important;word-wrap: break-word !important;text-align: justify;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">Lee\u8ba4\u4e3a\uff0c\u8fd9\u662f\u5176\u7814\u7a76\u7684\u4e00\u4e2a\u91cd\u8981\u751f\u7269\u5b66\u53d1\u73b0\uff0c\u56e0\u4e3a\u5728\u8fd9\u4e2a\u5b9e\u9a8c\u4e4b\u524d\uff0c\u4eba\u4eec\u5e76\u4e0d\u77e5\u9053\u7eb9\u72b6\u4f53\u4e2d\u7684mGluR5\u53d7\u4f53\u662f\u5426\u4e0e\u5938\u5f20\u6027\u91cd\u590d\u884c\u4e3a\u6709\u5173\u3002<\/span><\/p>\n<p style=\"margin-right: 16px;margin-left: 16px;max-width: 100%;min-height: 1em;line-height: 2em;text-indent: 2em;box-sizing: border-box !important;word-wrap: break-word !important;text-align: justify;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u5728\u60a3\u6709FXS\u7684\u5c0f\u9f20\u4e2d\uff0c<span style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\u5938\u5f20\u6027\u91cd\u590d\u884c\u4e3a<\/span>\u5305\u62ec\u5f3a\u8feb\u6027\u6316\u6398\u548c\u5468\u671f\u6027\u8df3\u8dc3\u5230\u7a7a\u4e2d\u3002\u5f53\u8f7d\u6709Cas9\u590d\u5408\u7269\u7684CRISPR-Gold\u6ce8\u5165\u5927\u8111\u7eb9\u72b6\u4f53\u4e4b\u540e\uff0c\u5c0f\u9f20\u7684\u6316\u6398\u6b21\u6570\u51cf\u5c11\u4e86\u7ea630\uff05\uff0c\u800c\u8df3\u8dc3\u6b21\u6570\u51cf\u5c11\u4e86\u7ea670\uff05\u3002\u4e0e\u6b64\u540c\u65f6\uff0c\u5927\u7ea650\uff05\u7684\u7eb9\u72b6\u4f53\u4e2d\u7684mGluR5\u88ab\u57fa\u56e0\u7f16\u8f91\u8fc7\uff0c\u4f7f\u53d7\u4f53\u86cb\u767d\u7684\u6570\u91cf\u51cf\u5c11\u4e86\u8fd1\u4e00\u534a\u3002<\/span><\/p>\n<p style=\"margin-right: 16px;margin-left: 16px;max-width: 100%;min-height: 1em;text-indent: 2em;line-height: 2em;box-sizing: border-box !important;word-wrap: break-word !important;text-align: justify;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u5c3d\u7ba1\u4e4b\u524d\u6709\u5236\u836f\u516c\u53f8\u5c1d\u8bd5\u5c06\u5c0f\u5206\u5b50\u836f\u7269\u6ce8\u5c04\u5230\u8840\u6db2\u7cfb\u7edf\u4e2d\u4ee5\u963b\u65ad\u76f8\u540c\u7684\u53d7\u4f53\uff0c\u4f46\u662f\u7814\u7a76\u4eba\u5458\u53d1\u73b0\uff0c\u867d\u7136\u5c0f\u9f20\u7684\u91cd\u590d\u884c\u4e3a\u5f97\u5230\u4e86\u5c11\u91cf\u7684\u63a7\u5236\uff0c\u4f46\u662f\u5728\u968f\u540e\u7684\u6ce8\u5c04\u6cbb\u7597\u4e2d\uff0c\u60a3\u6709FXS\u7684\u5c0f\u9f20\u4ea7\u751f\u4e86\u8010\u836f\u6027\u3002<\/span><\/p>\n<p style=\"margin-right: 16px;margin-left: 16px;max-width: 100%;min-height: 1em;text-indent: 2em;line-height: 2em;box-sizing: border-box !important;word-wrap: break-word !important;text-align: justify;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;box-sizing: border-box !important;word-wrap: break-word !important;text-align: justify;\"><img class=\"\"       style=\"box-sizing: border-box !important;word-wrap: break-word !important;width: auto !important;visibility: visible !important;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/08\/beepress0-1533690526-1.jpeg\" alt=\"\u7eb3\u7c73\u9897\u7c92\u4f20\u9012\u6280\u672f\u52a9\u529bCRISPR\u6cbb\u7597\u81ea\u95ed\u75c7\" title=\"\u7eb3\u7c73\u9897\u7c92\u4f20\u9012\u6280\u672f\u52a9\u529bCRISPR\u6cbb\u7597\u81ea\u95ed\u75c7\"  \/><\/p>\n<p style=\"margin-right: 16px;margin-left: 16px;max-width: 100%;min-height: 1em;text-indent: 2em;line-height: 2em;box-sizing: border-box !important;word-wrap: break-word !important;text-align: justify;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"margin-right: 16px;margin-left: 16px;max-width: 100%;min-height: 1em;text-indent: 2em;line-height: 2em;box-sizing: border-box !important;word-wrap: break-word !important;text-align: justify;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u7531Murthy\u5f00\u53d1\u7684CRISPR-Gold\u7cfb\u7edf\u4e3b\u8981\u4e13\u6ce8\u4e8e\u836f\u7269\u4f20\u9012\u548c\u65b0\u6297\u751f\u7d20\u7684\u5f00\u53d1\u3002\u8be5\u6280\u672f\u901a\u8fc7DNA\u94fe\u8986\u76d6\u7684\u91d1\u7eb3\u7c73\u7c92\u5b50\u7ea6\u675fCas9\u590d\u5408\u7269\uff0c\u5305\u62ec\u4e00\u4e2a\u57fa\u56e0\u5207\u5272\u9176\u548c\u4e00\u4e2a\u5411\u5bfcRNA\u3002\u8fd9\u4e2a\u7ec4\u5408\u88ab\u5c01\u88c5\u5728\u805a\u5408\u7269\u91cc\uff0c\u6709\u52a9\u4e8e\u8fdb\u5165\u5408\u9002\u7684\u7ec6\u80de\u3002<\/span><\/p>\n<p style=\"margin-right: 16px;margin-left: 16px;max-width: 100%;min-height: 1em;text-indent: 2em;line-height: 2em;box-sizing: border-box !important;word-wrap: break-word !important;text-align: justify;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u53bb\u5e74\uff0cMurthy\u53ca\u5176\u540c\u4e8b\u8bc1\u5b9e\u4e86CRISPR-Gold\u53ef\u4ee5\u5c06Cas9\u8f6c\u8fd0\u5230\u808c\u8089\u7ec6\u80de\u4e2d\uff0c\u5e76\u53ef\u4ee5\u7528\u6b63\u5e38\u57fa\u56e0\u53d6\u4ee3\u53d8\u5f02\u57fa\u56e0\uff0c\u4ee5\u63d0\u9ad8\u60a3\u675c\u6c0f\u808c\u8425\u517b\u4e0d\u826f\u75c7\u7684\u5c0f\u9f20\u7684\u529b\u91cf\u3002\u53e6\u4e00\u7bc7\u8bba\u6587\u4e5f\u8bc1\u660e\u4e86\u53ef\u4ee5\u901a\u8fc7\u8fd0\u7528CRISPR-Gold\u6210\u529f\u5730\u5c06Cas9\u5bfc\u5165\u5927\u8111\u4e2d\u7684\u5404\u79cd\u7ec6\u80de\u3002<\/span><\/p>\n<p style=\"margin-right: 16px;margin-left: 16px;max-width: 100%;min-height: 1em;text-indent: 2em;line-height: 2em;box-sizing: border-box !important;word-wrap: break-word !important;text-align: justify;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">Murthy\u8ba4\u4e3a\uff0c\u7814\u7a76\u4eba\u5458\u8fd8\u80fd\u591f\u901a\u8fc7CRISPR-Gold\u7f16\u8f91\u975e\u795e\u7ecf\u5143\uff1a\u5c0f\u80f6\u8d28\u7ec6\u80de\uff08\u5927\u8111\u514d\u75ab\u7cfb\u7edf\u7684\u4e00\u90e8\u5206\uff09\uff0c\u4ee5\u53ca\u7528\u6765\u652f\u6301\u795e\u7ecf\u5143\u7684\u661f\u5f62\u80f6\u8d28\u7ec6\u80de\u3002\u6bd4\u8d77\u795e\u7ecf\u5143\uff0c\u7814\u7a76\u4eba\u5458\u53ef\u4ee5\u66f4\u6709\u6548\u5730\u7f16\u8f91\u5b83\u4eec\uff0c\u5e76\u5728\u5bf9\u8bb8\u591a\u75be\u75c5\u7684\u7814\u7a76\u548c\u6cbb\u7597\u8fc7\u7a0b\u4e2d\u53d1\u6325\u975e\u5e38\u91cd\u8981\u7684\u4f5c\u7528\u3002<\/span><\/p>\n<p style=\"margin-right: 16px;margin-left: 16px;max-width: 100%;min-height: 1em;text-indent: 2em;line-height: 2em;box-sizing: border-box !important;word-wrap: break-word !important;text-align: justify;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">2016\u5e74\uff0c\u4e3a\u6d4b\u8bd5CRISPR-Gold\u5bf9\u4e0d\u540c\u75be\u75c5\u7684\u7597\u6548\uff0cMurthy\u7684\u5b9e\u9a8c\u5ba4\u53e6\u5916\u521b\u7acb\u4e86\u4e00\u5bb6\u53ebGenEdit\u7684\u516c\u53f8\u3002GenEdit\u9996\u5e2d\u6267\u884c\u5b98\u517c\u52a0\u5dde\u5927\u5b66\u4f2f\u514b\u5229\u5206\u6821\u535a\u58eb\uff0c\u6b63\u5728\u52a0\u5dde\u5927\u5b66\u4f2f\u514b\u5229\u5206\u6821QB3\u8f66\u5e93\u5de5\u4f5c\u7684Kunwoo Lee\u6210\u529f\u7f16\u8f91\u4e86\u8111\u7ec6\u80de\uff0c\u4e3a\u6cbb\u7597\u8bb8\u591a\u75be\u75c5\u6253\u5f00\u4e86\u5927\u95e8\u3002<\/span><\/p>\n<p style=\"margin-right: 16px;margin-left: 16px;max-width: 100%;min-height: 1em;text-indent: 2em;line-height: 2em;box-sizing: border-box !important;word-wrap: break-word !important;text-align: justify;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">Lee\u8ba4\u4e3a\uff0cCRISPR-Gold\u53ef\u7528\u4e8e\u6cbb\u7597\u5404\u79cd\u9057\u4f20\u6027\u75be\u75c5\uff0c\u5982\u4ea8\u5ef7\u987f\u75c5\uff0c\u4f46\u5b83\u4e0d\u4ec5\u4ec5\u9650\u4e8e\u5355\u57fa\u56e0\u75be\u75c5\u3002\u4e00\u65e6\u7814\u7a76\u4eba\u5458\u5f04\u6e05\u695a\u6d89\u53ca\u6574\u4e2a\u57fa\u56e0\u7684\u7f51\u7edc\uff0cCIRISPR-Gold\u4e5f\u53ef\u7528\u4e8e\u5bf9\u6297\u591a\u57fa\u56e0\u75be\u75c5\u3002<\/span><\/p>\n<p style=\"margin-right: 16px;margin-left: 16px;max-width: 100%;min-height: 1em;line-height: 2em;text-indent: 2em;box-sizing: border-box !important;word-wrap: break-word !important;text-align: justify;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u5bf9\u4e8e\u8fd9\u9879\u7814\u7a76\u91cd\u8981\u7684\u4e00\u6b65\uff0cMurthy\u8ba4\u4e3a\uff0c\u662fGenEdit\u60f3\u51fa\u4e86\u5982\u4f55\u901a\u8fc7\u957f\u8ddd\u79bb\u7684\u8fd0\u9001CRISPR-Gold\u9897\u7c92\uff08\u4ece\u4f2f\u514b\u5229\u5230\u5723\u5b89\u4e1c\u5c3c\u5965\uff09\u91cd\u590d\u5236\u9020\u5b83\u4eec\uff0c\u5e76\u6d88\u9664\u4e00\u4e2a\u9650\u5236\u7eb3\u7c73\u6280\u672f\u8f6c\u5316\u7684\u5173\u952e\u74f6\u9888\u3002<\/span><\/p>\n<p style=\"margin-right: 16px;margin-left: 16px;max-width: 100%;min-height: 1em;line-height: 2em;text-indent: 2em;box-sizing: border-box !important;word-wrap: break-word !important;text-align: justify;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">Hye Young Lee\uff0cKunwoo Lee\u548cMurthy\u76ee\u524d\u6b63\u81f4\u529b\u4e8e\u5f00\u53d1\u901a\u8fc7\u810a\u9ad3\u76f4\u63a5\u6ce8\u5165\u4e2d\u67a2\u795e\u7ecf\u7cfb\u7edf\u7684CRISPR-Gold\u9897\u7c92\uff0c\u4ee5\u907f\u514d\u9700\u8981\u6253\u5f00\u9885\u9aa8\u5e76\u76f4\u63a5\u6ce8\u5165\u5927\u8111\u3002Hye Young Lee\u4e50\u89c2\u5730\u8ba4\u4e3a\u8fd9\u53ef\u80fd\u4e0e\u9885\u5185\u6ce8\u5c04Cas9\u590d\u5408\u7269\u5230\u7eb9\u72b6\u4f53\u4ee5\u51cf\u5c11\u91cd\u590d\u884c\u4e3a\u7684\u65b9\u6cd5\u4e00\u6837\u6709\u6548\uff0c\u751a\u81f3\u53ef\u80fd\u66f4\u6709\u6548\u7684\u89e3\u51b3ASD\u60a3\u8005\u7684\u793e\u4f1a\u4ea4\u5f80\u95ee\u9898\u3002<\/span><\/p>\n<p style=\"margin-right: 16px;margin-left: 16px;max-width: 100%;min-height: 1em;line-height: 2em;box-sizing: border-box !important;word-wrap: break-word !important;text-align: justify;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u6765\u6e90\uff1a<em style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">Nature Biomedical Engineering<\/em><\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;text-align: center;box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;font-size: 20px;font-family: -apple-system-font, BlinkMacSystemFont, Arial, sans-serif;letter-spacing: 0.544px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;color: rgb(255, 0, 0);box-sizing: border-box !important;word-wrap: break-word !important;\">\u8bc6\u522b\u5fae\u4fe1\u4e8c\u7ef4\u7801\uff0c\u6dfb\u52a0\u751f\u7269\u5236\u54c1\u5708\u5c0f\u7f16\uff0c\u7b26\u5408\u6761\u4ef6\u8005\u5373\u53ef\u52a0\u5165\u751f\u7269\u5236\u54c1\u5708\u5fae\u4fe1\u7fa4\uff01<\/span><\/strong><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;font-family: -apple-system-font, BlinkMacSystemFont, Arial, sans-serif;text-align: center;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 18px;box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;color: rgb(0, 209, 0);box-sizing: border-box !important;word-wrap: break-word !important;\">\u8bf7\u6ce8\u660e\uff1a\u59d3\u540d+\u7814\u7a76\u65b9\u5411\uff01<\/span><\/strong><\/span><\/p>\n<section  label=\"Powered by 135editor.com\" style=\"max-width: 100%;font-size: 16px;letter-spacing: 0.544px;font-family: -apple-system-font, BlinkMacSystemFont, Arial, sans-serif;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<p style=\"max-width: 100%;min-height: 1em;text-align: center;box-sizing: border-box !important;word-wrap: break-word !important;\"><img class=\"\"      width=\"269px\"  style=\"box-sizing: border-box !important;word-wrap: break-word !important;visibility: visible !important;width: 269px !important;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/08\/beepress6-1533690526.jpeg\" alt=\"\u7eb3\u7c73\u9897\u7c92\u4f20\u9012\u6280\u672f\u52a9\u529bCRISPR\u6cbb\u7597\u81ea\u95ed\u75c7\" title=\"\u7eb3\u7c73\u9897\u7c92\u4f20\u9012\u6280\u672f\u52a9\u529bCRISPR\u6cbb\u7597\u81ea\u95ed\u75c7\"  \/><\/p>\n<\/section>\n<p><br  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;font-size: 16px;letter-spacing: 0.544px;line-height: 25.6px;font-family: \u5fae\u8f6f\u96c5\u9ed1;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;line-height: 24px;box-sizing: border-box !important;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/span><\/p>\n<section style=\"max-width: 100%;font-size: 16px;letter-spacing: 0.544px;text-align: center;line-height: 25.6px;font-family: \u5fae\u8f6f\u96c5\u9ed1;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section style=\"margin-top: 0.5em;margin-bottom: 0.5em;max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section style=\"margin: -0.9em auto 0.9em;max-width: 100%;width: 0.6em;height: 0.6em;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section style=\"margin-left: 1px;max-width: 100%;border-radius: 100%;width: 9.59px;height: 9.59px;background-color: rgb(170, 33, 94);box-sizing: border-box !important;word-wrap: break-word !important;\"><br  style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"max-width: 100%;letter-spacing: 0.544px;font-size: 14px;text-align: center;font-family: \u5fae\u8f6f\u96c5\u9ed1;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;padding-left: 4px;max-width: 100%;text-align: justify;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section style=\"margin-bottom: 0.2em;max-width: 100%;width: 666px;vertical-align: bottom;display: inline-block;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;width: 599.39px;border-top: 1px solid rgb(170, 33, 94);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/section>\n<section style=\"margin-top: -4px;margin-right: -4px;max-width: 100%;border-radius: 100%;width: 8px;height: 8px;background-color: rgb(170, 33, 94);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/section>\n<\/section>\n<section style=\"margin-top: -1.5em;max-width: 100%;text-align: right;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;border-radius: 100%;width: 2em;height: 2em;text-align: center;color: rgb(255, 255, 255);line-height: 2em;vertical-align: top;display: inline-block;background-color: rgb(170, 33, 94);box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;color: rgb(170, 33, 94);box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 18px;box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><\/strong><\/span>\u7248<\/section>\n<\/section>\n<section style=\"max-width: 100%;border-radius: 100%;width: 2em;height: 2em;text-align: center;color: rgb(255, 255, 255);line-height: 2em;vertical-align: top;display: 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100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\u660e<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section  label=\"Powered by 135editor.com\" style=\"max-width: 100%;font-size: 16px;letter-spacing: 0.544px;font-family: -apple-system-font, BlinkMacSystemFont, Arial, sans-serif;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 14px;color: rgb(105, 115, 117);font-family: \u5fae\u8f6f\u96c5\u9ed1;letter-spacing: 1px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u672c\u516c\u4f17\u53f7\u6240\u6709\u8f6c\u8f7d\u6587\u7ae0\u7cfb\u51fa\u4e8e\u4f20\u9012\u66f4\u591a\u4fe1\u606f\u4e4b\u76ee\u7684\uff0c\u4e14\u660e\u786e\u6ce8\u660e\u6765\u6e90\u548c\u4f5c\u8005\uff0c\u4e0d\u5e0c\u671b\u88ab\u8f6c\u8f7d\u7684\u5a92\u4f53\u6216\u4e2a\u4eba\u53ef\u4e0e\u6211\u4eec\u8054\u7cfb(cbplib@163.com)\uff0c\u6211<span style=\"max-width: 100%;box-sizing: border-box 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