{"id":19345,"date":"2018-07-12T01:00:00","date_gmt":"2018-08-08T01:10:09","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/2018\/08\/08\/%e7%94%9f%e7%89%a9%e5%8a%9f%e8%83%bd%e5%ad%a6%e7%ad%9b%e9%80%89%e8%af%84%e4%bb%b7%e6%8a%80%e6%9c%af%e4%b9%8b%e4%ba%b2%e5%92%8c%e5%8a%9b%e8%af%84%e4%bb%b7%e7%af%87\/"},"modified":"2018-08-08T09:10:12","modified_gmt":"2018-08-08T01:10:12","slug":"%e7%94%9f%e7%89%a9%e5%8a%9f%e8%83%bd%e5%ad%a6%e7%ad%9b%e9%80%89%e8%af%84%e4%bb%b7%e6%8a%80%e6%9c%af%e4%b9%8b%e4%ba%b2%e5%92%8c%e5%8a%9b%e8%af%84%e4%bb%b7%e7%af%87","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/19345\/","title":{"rendered":"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u6280\u672f\u4e4b\u4eb2\u548c\u529b\u8bc4\u4ef7\u7bc7"},"content":{"rendered":"
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\u6458\u8981\uff1a<\/section>\n<\/section>\n<\/section>\n<\/section>\n

<\/span><\/h3>\n

\u672c\u6587\u7684\u4e3b\u8981\u76ee\u7684\u662f\u5bf9\u836f\u7269\u7814\u53d1\u8fc7\u7a0b\u4e2d\u4eb2\u548c\u529b\u8bc4\u4ef7\u5b9e\u9a8c\u65b9\u6cd5\u8fdb\u884c\u7b80\u5355\u6982\u62ec\u548c\u603b\u7ed3\u3002\u4e3b\u8981\u5185\u5bb9\u5305\u62ec\u660e\u786e\u4eb2\u548c\u529b\u7684\u6982\u5ff5\uff0c\u7b80\u5355\u9610\u8ff0\u4eb2\u548c\u529b\u5173\u8054\u7684\u52a8\u529b\u5b66\uff0c\u70ed\u529b\u5b66\u5316\u5b66\u539f\u7406\uff0c\u6982\u8ff0\u4ee5\u5176\u5316\u5b66\u539f\u7406\u4e3a\u89c6\u89d2\u7684\u51e0\u79cd\u5e38\u7528\u7684\u4eb2\u548c\u529b\u8bc4\u4ef7\u65b9\u6cd5\uff0c\u5206\u6790\u5404\u79cd\u4eb2\u548c\u529b\u8bc4\u4ef7\u65b9\u6cd5\u95f4\u7684\u53ef\u6bd4\u6027\uff0c\u5e76\u5bf9\u4eb2\u548c\u529b\u8fdb\u884c\u65b0\u836f\u7814\u53d1\u521d\u671f\u7684\u9ad8\u901a\u91cf\u7b5b\u9009\u548c\u7279\u6b8a\u5e94\u7528\u505a\u521d\u6b65\u7684\u5c55\u671b\u3002<\/span><\/p>\n

\u5728\u7ebf\u57f9\u8bad\u63a8\u8350\uff1a<\/span>\u5728\u7ebf\u4ea4\u6d41|\u4f53\u5916\u751f\u7269\u5b66\u65b9\u6cd5\u5f00\u53d1-\u57fa\u672c\u539f\u7406\u548c\u5b9e\u8df5<\/a><\/span><\/span><\/p>\n

<\/span><\/h2>\n
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\u4eb2\u548c\u529b\u6982\u5ff5<\/section>\n<\/section>\n<\/section>\n<\/section>\n

<\/span><\/h2>\n

\u201c\u6709\u7ed3\u5408\u624d\u4f1a\u6709\u6709\u529f\u80fd\uff0c\u6709\u529f\u80fd\u4e00\u5b9a\u6709\u7ed3\u5408\u3002\u201dBinding(\u7ed3\u5408)\u548cFunction\uff08\u529f\u80fd\uff09\u662f\u4f53\u5916\u836f\u6548\u521d\u6b65\u8bc4\u4ef7\u4e2d\u6700\u91cd\u8981\u7684\u4e24\u73af\uff0c\u5bf9\u4e8e\u5019\u9009\u836f\u7269\u6765\u8bf4\uff0c\u7ed3\u5408\u662f\u5176\u836f\u7269\u53d1\u6325\u529f\u80fd\u7684\u5fc5\u8981\u6761\u4ef6\uff0c\u4f46\u5e76\u975e\u5145\u5206\u7684\u6761\u4ef6\u3002\u8bc4\u4ef7\u4e00\u4e2a\u5019\u9009\u836f\u7269\uff0c\u5176\u4e0e\u9776\u70b9\u5206\u5b50\u7684\u7ed3\u5408\u80fd\u529b\u662f\u6700\u57fa\u7840\u4e5f\u662f\u6700\u91cd\u8981\u7684\u4e00\u65b9\u9762\u3002\u836f\u7269\u548c\u9776\u70b9\u5206\u5b50\u7684\u7ed3\u5408\u53ef\u5206\u4e3a\u4e0d\u53ef\u9006\u7ed3\u5408\u548c\u53ef\u9006\u7ed3\u5408\uff0c\u4e0a\u5e02\u836f\u7269\u4e2d\uff0c\u7edd\u5927\u6570\u836f\u7269\u548c\u9776\u70b9\u5206\u5b50\u7684\u7ed3\u5408\u53ef\u9006\u7ed3\u5408\uff0c\u8fd9\u6709\u5229\u4e8e\u836f\u7269\u8fdb\u5165\u4eba\u4f53\u5185\u540e\u80fd\u591f\u66f4\u5bb9\u6613\u7684\u4ee3\u8c22\uff0c\u51cf\u5c11\u53ef\u80fd\u5b58\u5728\u7684\u957f\u671f\u84c4\u79ef\u548c\u5b89\u5168\u6027\u98ce\u9669\u3002\u4e0d\u8bba\u5c0f\u5206\u5b50\u836f\u7269\u8fd8\u662f\u5927\u5206\u5b50\u836f\u7269\u7684\u7814\u53d1\u8fc7\u7a0b\u4e2d\u5747\u4f1a\u4f7f\u7528\u4eb2\u548c\u529b(affinity)\u6765\u8bc4\u4ef7\u5019\u9009\u5206\u5b50\u548c\u9776\u70b9\u5206\u5b50\u4e4b\u95f4\u7ed3\u5408\u7684\u5f3a\u5f31\uff0c\u8fd9\u91cc\u9700\u8981\u660e\u786e\u7684\u662f\u4eb2\u548c\u529b(<\/strong>affinity<\/strong>)\u662f\u53ef\u9006\u53cd\u5e94\u8fc7\u7a0b\u4e2d\u5019\u9009\u5206\u5b50\uff0c\u9776\u70b9\u5206\u5b50\u8868\u4f4d\u548c\u5019\u9009\u5206\u5b50-\u9776\u70b9\u5206\u5b50\u7ed3\u5408\u7269\u4e4b\u95f4\u7684\u76f8\u5bf9\u72b6\u6001\u7684\u7279\u5f81\u51fd\u6570\uff0c\u5176\u66f4\u52a0\u4e13\u4e1a\u6027\u548c\u672f\u8bed\u5316\u7684\u540d\u79f0\u662f\u89e3\u79bb\u5e73\u8861\u5e38\u6570K<\/strong>D<\/span><\/sub><\/strong>\uff0c\u5355\u4f4dmol\/L(\u548c\u6d53\u5ea6\u5355\u4f4d\u4e00\u81f4)\u3002<\/strong>\u5982\u679c\u4e00\u4e2a\u9776\u70b9\u63d0\u4f9b\u591a\u4e2a\u7ed3\u5408\u4f4d\u70b9\uff0c\u6709\u65f6\u5019\u4e5f\u79f0\u8fd9\u79cd\u7ed3\u5408\u5f3a\u5f31\u4e3a\u7ed3\u5408\u529b\uff08A<\/span>vidity<\/span>\uff09\u3002\u4e00\u822c\u6765\u8bb2\uff0c\u5019\u9009\u5206\u5b50\u548c\u9776\u70b9\u5206\u5b50\u7684\u7ed3\u5408\u8d8a\u5f3a\uff0c\u5373\u4eb2\u548c\u529b\u8d8a\u5927\uff0c\u5176\u89e3\u79bb\u5e73\u8861\u5e38\u6570\u8d8a\u5c0f\u3002\u89e3\u79bb\u5e73\u8861\u5e38\u6570\u7684\u5355\u4f4d\u662f\u6d53\u5ea6\u5355\u4f4d\uff0c\u8fd9\u4e2a\u548c\u5728\u836f\u7269\u7814\u53d1\u8fc7\u7a0b\u4e2d\u8fdb\u884c\u751f\u7269\u5b66\u529f\u80fd\u8bc4\u4ef7\u5e38\u5e38\u4f1a\u5f97\u5230IC50,EC50\u7b49\u5355\u4f4d\u4e00\u81f4\uff0c\u4e5f\u5e38\u5e38\u4f1a\u4f7f\u5f97\u8fd9\u4e9b\u4e13\u6709\u540d\u8bcd\u51fa\u73b0\u4e71\u7528\uff0c\u6df7\u7528\u4e43\u81f3\u76f8\u4e92\u6bd4\u8f83\u7684\u60c5\u51b5\uff0c\u4ece\u800c\u5f97\u5230\u9519\u8bef\u7684\u7ed3\u8bba\u3002\u5982\u679c\u6211\u4eec\u9700\u8981\u6bd4\u8f83\u900f\u5f7b\u7684\u7406\u89e3\u4eb2\u548c\u529b\u7684\u6982\u5ff5\uff0c\u9996\u5148\u5f97\u4ece\u53ef\u9006\u53cd\u5e94\u8bb2\u8d77\u3002<\/span><\/p>\n

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\u53ef\u9006\u53cd\u5e94<\/section>\n<\/section>\n<\/section>\n<\/section>\n

\u5728\u751f\u7269\u4f53\u5185\uff0c\u53ef\u9006\u53cd\u5e94\u662f\u6700\u5e38\u89c1\u4e5f\u662f\u6700\u91cd\u8981\u7684\u5316\u5b66\u53cd\u5e94\u8fc7\u7a0b\uff0c\u914d\u4f53\u548c\u53d7\u4f53\u7684\u53cd\u5e94\uff0c\u6297\u4f53\u548c\u6297\u539f\u7684\u53cd\u5e94\uff0c\u9176\u548c\u5e95\u7269\u53cd\u5e94\u90fd\u662f\u53ef\u9006\u53cd\u5e94\u7684\u8fc7\u7a0b\u3002\u53ef\u9006\u53cd\u5e94\u8fc7\u7a0b\u4e2d\u4ee5\u6297\u539f\u548c\u6297\u4f53\u7684\u53cd\u5e94\u4e3a\u4f8b\uff0c\u65e2\u6709\u6b63\u76f8\u7684\u53cd\u5e94\u8fc7\u7a0b\uff0c\u5373\u6297\u539f\u548c\u6297\u4f53\u7ed3\u5408\u53cd\u5e94\u751f\u6210\u6297\u539f-\u6297\u4f53\u590d\u5408\u7269\u7684\u6b63\u53cd\u5e94\u8fc7\u7a0b<\/span><\/strong>\uff0c<\/strong>\u4e5f\u6709\u9006\u5411\u53cd\u5e94\uff0c\u6297\u539f-\u6297\u4f53\u590d\u5408\u7269\u89e3\u79bb\u53cd\u5e94\u751f\u6210\u6297\u539f\uff0c\u6297\u4f53\u7684\u9006\u53cd\u5e94\u8fc7\u7a0b<\/span><\/strong>\uff0c\u5f53\u4e00\u4e2a\u53ef\u9006\u53cd\u5e94\u4e2d\u6b63\u53cd\u5e94\u548c\u9006\u53cd\u5e94\u7684\u901f\u5ea6\u76f8\u7b49\u65f6\uff0c\u53cd\u5e94\u4f53\u7cfb\u4e2d\u6297\u539f\u7684\u6d53\u5ea6\uff0c\u6297\u4f53\u7684\u6d53\u5ea6\u548c\u6297\u539f-\u6297\u4f53\u590d\u5408\u7269\u6d53\u5ea6\u4e0d\u518d\u589e\u52a0\u4e5f\u4e0d\u518d\u51cf\u5c11\uff0c\u53ef\u9006\u53cd\u5e94\u5b8c\u6210\uff0c\u53cd\u5e94\u4f53\u7cfb\u4e2d\u5404\u6210\u5206\u6d53\u5ea6\u5904\u4e8e\u52a8\u6001\u5e73\u8861<\/strong>\u3002\u9488\u5bf9\u540c\u4e00\u6297\u539f\uff0c\u4e0d\u540c\u7684\u6297\u4f53A\uff0cB\uff0c\u76f8\u540c\u5206\u5b50\u6d53\u5ea6\u7684\u6297\u4f53A\uff0cB\u548c\u540c\u4e00\u5206\u5b50\u6d53\u5ea6\u7684\u6297\u539f\u53cd\u5e94\uff0c\u5982\u679cA\u6297\u4f53\u76f8\u5bf9\u4e8e\u6297\u539f\u7684\u4eb2\u548c\u529b\u8981\u5f3a\u4e8eB\u6297\u4f53\uff0c\u90a3\u4e48\u53ef\u9006\u53cd\u5e94\u5b8c\u6210\u5404\u7269\u8d28\u5f62\u6210\u52a8\u6001\u5e73\u8861\u540e\uff0cA\u6297\u4f53-\u6297\u539f\u590d\u5408\u7269\u7684\u6d53\u5ea6\u8981\u6bd4B\u6297\u4f53-\u6297\u539f\u590d\u5408\u7269\u6d53\u5ea6\u9ad8\uff0c\u4e5f\u53ef\u4ee5\u8bf4\u6297\u539f\u63d0\u4f9b\u7684\u7ed3\u5408\u4f4d\u70b9\u4e2d\uff0cA\u5360\u636e\u7684\u4f4d\u70b9\u8981\u6bd4B\u5360\u636e\u7684\u4f4d\u70b9\u8981\u591a\u3002\u8fd9\u53ea\u662f\u5bf9\u4e8e\u53ef\u9006\u53cd\u5e94\u548c\u4eb2\u548c\u529b\u7b80\u5355\u7684\u63cf\u8ff0\uff0c\u600e\u4e48\u5c06\u4eb2\u548c\u529b\u8fdb\u884c\u91cf\u5316\u8fdb\u884c\u6bd4\u8f83\uff0c\u5728\u65e0\u673a\u5316\u5b66\uff0c\u751f\u7269\u5b66\u548c\u836f\u7269\u7814\u53d1\u8fc7\u7a0b\u4e2d\u5bf9\u4e8e\u4eb2\u548c\u529b\u7684\u68c0\u6d4b\u5df2\u7ecf\u6709\u4e86\u5f88\u591a\u6d4b\u91cf\u65b9\u6cd5\u3002<\/span><\/p>\n

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\u4eb2\u548c\u529b\u68c0\u6d4b\u7684\u5316\u5b66\u539f\u7406<\/section>\n<\/section>\n<\/section>\n<\/section>\n

   <\/strong>\u57fa\u7840\u7814\u7a76\u5bf9\u4e8e\u53ef\u9006\u53cd\u5e94\u7684\u8fc7\u7a0b\uff0c\u4ee5\u53ca\u5176\u5316\u5b66\u52a8\u529b\u5b66\uff0c\u70ed\u529b\u5b66\u539f\u7406\u5df2\u7ecf\u6709\u6bd4\u8f83\u6e05\u6670\u7684\u7814\u7a76\uff0c\u5047\u8bbeR\uff08\u53d7\u4f53\uff09\u548cL\uff08\u914d\u4f53\uff09\u662f\u5355\u4ef7\u53cd\u5e94\uff0c\u5982\u4e0b\u56fe\u6240\u793a\uff0c\u53ef\u4ee5\u7528\u4e0b\u9762\u51e0\u4e2a\u6570\u5b66\u516c\u5f0f\u8fdb\u884c\u6982\u62ec1<\/span><\/sup>\uff1a<\/span><\/p>\n


<\/p>\n

\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u6280\u672f\u4e4b\u4eb2\u548c\u529b\u8bc4\u4ef7\u7bc7\"<\/span><\/p>\n

\u00b4 <\/span>K<\/span><\/strong>on\uff08<\/span><\/sub><\/strong>K<\/span><\/strong>a<\/span><\/sub><\/strong>\uff09<\/span><\/sub><\/strong>:\u7ed3\u5408<\/span><\/strong>\u901f\u7387<\/span><\/strong>\u5e38\u6570\uff0c<\/span><\/strong>\u7528\u4e8e\u8bc4\u4f30R\uff08\u53d7\u4f53\uff09\u548cL\uff08\u914d\u4f53\uff09\u7ed3\u5408\u5f62\u6210RL\uff08\u53d7\u4f53\u914d\u4f53\u590d\u5408\u7269\uff09\u901f\u7387\u5feb\u6162\u7684\u5e38\u6570\uff0c<\/span>R\u548cL\u6b63\u53cd\u5e94\u901f\u5ea6V=<\/span><\/strong>[<\/span><\/strong>R<\/span><\/strong>]<\/span><\/strong>f<\/span><\/sub><\/strong> <\/span><\/strong>*<\/span><\/strong> [L]<\/span><\/strong>f<\/span><\/sub><\/strong> <\/span><\/strong>*<\/span><\/strong>K<\/span><\/strong>on<\/span><\/sub><\/strong>\uff08<\/span><\/strong>\u52a8\u529b\u5b66\u53c2\u6570<\/span><\/strong>\uff09<\/span><\/strong><\/p>\n

\u00b4 <\/span>K<\/span><\/strong>off\uff08<\/span><\/sub><\/strong>K<\/span><\/strong>d<\/span><\/sub><\/strong>\uff09<\/span><\/sub><\/strong>: \u89e3\u79bb<\/span><\/strong>\u901f\u7387<\/span><\/strong>\u5e38\u6570\uff0c<\/span><\/strong>\u7528\u4e8e\u8bc4\u4f30RL\uff08\u53d7\u4f53\u914d\u4f53\u590d\u5408\u7269\uff09\u9006\u5411\u89e3\u79bb\u5f62\u6210R\uff08\u53d7\u4f53\uff09\u548cL\uff08\u914d\u4f53\uff09\u901f\u7387\u5feb\u6162\u7684\u5e38\u6570\uff0c<\/span> RL\u89e3\u79bb\u5f62\u6210R\u548cL\u7684\u9006\u53cd\u5e94\u901f\u5ea6V=<\/span><\/strong>[<\/span><\/strong>R<\/span><\/strong>]<\/span><\/strong>f<\/span><\/sub><\/strong> <\/span><\/strong>*<\/span><\/strong> [L]<\/span><\/strong>f<\/span><\/sub><\/strong> <\/span><\/strong>*<\/span><\/strong> K<\/span><\/strong>off<\/span><\/sub><\/strong>\u3002\uff08<\/span><\/strong>\u52a8\u529b\u5b66\u53c2\u6570<\/span><\/strong>\uff09<\/span><\/strong><\/p>\n

\u00b4 <\/span>K<\/span><\/strong>A<\/span><\/sub><\/strong> <\/span><\/strong>:<\/span><\/sub><\/strong>\u7ed3\u5408<\/span><\/strong>\u5e73\u8861<\/span><\/strong>\u5e38\u6570\uff1a<\/span><\/strong>\u7528\u4e8e\u8bc4\u4f30\u53ef\u9006\u53cd\u5e94\u5f3a\u5f31\u7684\u5e38\u6570\u53c2\u6570\uff0c\u8fdb\u5165\u5e73\u8861\u72b6\u6001\u65f6\uff0c\u53cd\u5e94\u4f53\u7cfb\u4e2d\u81ea\u7531\u7684R\uff08\u53d7\u4f53\uff09\uff0c\u81ea\u7531\u7684L\uff08\u914d\u4f53\uff09\u548cRL\uff08\u53d7\u4f53\u914d\u4f53\u590d\u5408\u7269\uff09\u4e09\u8005\u4e4b\u95f4\u76f8\u5173\u5173\u7cfb\u7684\u5e38\u6570\u3002<\/span>\u8be5\u5e38\u6570\u8d8a\u5927\uff0c\u8bf4\u660eR\uff08\u53d7\u4f53\uff09\u548cL\uff08\u914d\u4f53\uff09\u7684\u4eb2\u548c\u529b\u8d8a\u5f3a\u3002<\/span><\/strong><\/p>\n

\u00b4 <\/span>K<\/span><\/strong>D<\/span><\/sub><\/strong>:\u89e3\u79bb\u5e73\u8861\u5e38\u6570<\/span><\/strong>\uff1a\u7528\u4e8e\u8bc4\u4f30\u53ef\u9006\u53cd\u5e94\u5f3a\u5f31\u7684\u5e38\u6570\u53c2\u6570\uff0c\u8fdb\u5165\u5e73\u8861\u72b6\u6001\u65f6\uff0c\u53cd\u5e94\u4f53\u7cfb\u4e2d\u81ea\u7531\u7684R\uff08\u53d7\u4f53\uff09\uff0c\u81ea\u7531\u7684L\uff08\u914d\u4f53\uff09\u548cRL\uff08\u53d7\u4f53\u914d\u4f53\u590d\u5408\u7269\uff09\u4e09\u8005\u4e4b\u95f4\u76f8\u5173\u5173\u7cfb\u7684\u5e38\u6570<\/span>\u3002\u7ed3\u5408\u5e73\u8861\u5e38\u6570\u7684\u5012\u6570\uff0c\u8be5\u5e38\u6570\u8d8a\u5c0f\uff0c\u8bf4\u660eR\uff08\u53d7\u4f53\uff09\u548cL\uff08\u914d\u4f53\uff09\u7684\u4eb2\u548c\u529b\u8d8a\u5f3a\uff0c\u5355\u4f4d\u4e3amol\/L,\u548c\u56e0\u4e3a\u6d53\u5ea6\u5355\u4f4d\u76f8\u540c\u4fbf\u4e8e\u6bd4\u8f83\u548c\u4e66\u5199\uff0c\u4e00\u822c\u7528\u4e8e\u8868\u793a\u4eb2\u548c\u529b\u7684\u5927\u5c0f\uff0c\u8be5\u5e38\u6570\u8d8a\u5c0f\uff0c\u8bf4\u660eR\uff08\u53d7\u4f53\uff09\u548cL\uff08\u914d\u4f53\uff09\u7684\u4eb2\u548c\u529b\u8d8a\u5f3a\u3002<\/span><\/strong><\/p>\n


<\/p>\n

\u4e09\u4e2a\u516c\u5f0f\u5206\u522b\u4ee3\u8868\u4e86\u4e09\u79cd\u8fdb\u884c\u4eb2\u548c\u529b\u68c0\u6d4b\u7684\u65b9\u6cd5\uff0c\u70ed\u529b\u5b66\u68c0\u6d4b\u65b9\u6cd5\uff08\u516c\u5f0f\u4e00\uff09\uff1b\u52a8\u529b\u5b66\u68c0\u6d4b\u65b9\u6cd5\uff08\u516c\u5f0f\u4e8c\uff09\u548c\u5e73\u8861\u52a8\u529b\u5b66\u68c0\u6d4b\u65b9\u6cd5\uff08\u516c\u5f0f\u4e09\uff09\uff0c\u90a3\u4e48\u4e09\u79cd\u68c0\u6d4b\u65b9\u6cd5\u5177\u4f53\u7684\u5b9e\u9a8c\u65b9\u6cd5\u53c8\u6709\u54ea\u4e9b\u5462\uff1f<\/span><\/strong><\/p>\n

<\/span><\/strong><\/h2>\n
\n
\n
\n
\u70ed\u529b\u5b66\u68c0\u6d4b\u65b9\u6cd5<\/section>\n<\/section>\n<\/section>\n<\/section>\n

<\/span><\/strong><\/h2>\n

\u7b49\u6e29\u6ef4\u5b9a\u91cf\u70ed\u6cd5\uff08ITC\uff09\u662f\u7528\u4e8e\u91cf\u5316\u7814\u7a76\u5404\u79cd\u751f\u7269\u5206\u5b50\u76f8\u4e92\u4f5c\u7528\u7684\u4e00\u79cd\u6280\u672f\u3002 \u5b83\u53ef\u76f4\u63a5\u6d4b\u91cf\u751f\u7269\u5206\u5b50\u7ed3\u5408\u8fc7\u7a0b\u4e2d\u91ca\u653e\u6216\u5438\u6536\u7684\u70ed\u91cf\u3002ITC\u662f\u80fd\u591f\u5728\u4e00\u6b21\u8bd5\u9a8c\u4e2d\u540c\u65f6\u786e\u5b9a\u591a\u79cd\u7ed3\u5408\u53c2\u6570\u7684\u6280\u672f\u3002 ITC\u53ef\u4ee5\u6d4b\u5b9a\u7ed3\u5408\u914d\u5076\u4f53\u5728\u81ea\u7136\u72b6\u6001\u4e0b\u7684\u4eb2\u548c\u529b\uff0c\u65e0\u9700\u901a\u8fc7\u8367\u5149\u6807\u8bb0\u6216\u56fa\u5b9a\u5316\u6280\u672f\u5bf9\u7ed3\u5408\u914d\u5076\u4f53\u8fdb\u884c\u4fee\u9970\u3002\u901a\u8fc7\u6d4b\u91cf\u7ed3\u5408\u8fc7\u7a0b\u4e2d\u7684\u70ed\u4f20\u9012\uff0c\u5c31\u80fd\u591f\u51c6\u786e\u5730\u786e\u5b9a\u7ed3\u5408\u5e38\u6570\uff08KD\uff09\u3001\u53cd\u5e94\u5316\u5b66\u91cf\uff08n\uff09\u3001\u7113\uff08\u2206H\uff09\u548c\u71b5\uff08\u0394S\uff09\u7b49\u3002\u5982\u4e0b\u56fe\u6240\u793a2<\/span><\/sup>\uff0c<\/span><\/p>\n

\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u6280\u672f\u4e4b\u4eb2\u548c\u529b\u8bc4\u4ef7\u7bc7\"<\/span><\/p>\n

<\/span><\/strong><\/h2>\n
\n
\n
\n
\u52a8\u529b\u5b66\u68c0\u6d4b\u65b9\u6cd5<\/section>\n<\/section>\n<\/section>\n<\/section>\n

<\/span><\/strong><\/h2>\n

\u91c7\u7528<\/span>\u8868\u9762\u7b49\u79bb\u5b50\u5171\u632f\u6280\u672f\uff0c\u82f1\u6587\u7b80\u5199SPR\uff0c\u662f\u4ece20\u4e16\u7eaa90\u5e74\u4ee3\u53d1\u5c55\u8d77\u6765\u7684\u4e00\u79cd\u65b0\u6280\u672f\uff0c\u5176\u5e94\u7528SPR\u539f\u7406\u68c0\u6d4b\u751f\u7269<\/span>\u4f20\u611f<\/span>\u82af\u7247\uff08biosensor chip\uff09\u4e0a\u914d\u4f53\u4e0e\u5206\u6790\u7269\u4e4b\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u60c5\u51b5<\/span>\uff0c\u8be5\u65b9\u6cd5\u80fd\u591f\u6d4b\u91cf\u914d\u4f53\u4e0e\u5206\u6790\u7269\u7684\u52a8\u529b\u5b66\u53c2\u6570<\/span>K<\/strong>on<\/span><\/sub><\/strong>:\u7ed3\u5408<\/strong>\u901f\u7387<\/strong>\u5e38\u6570\u548c<\/strong>K<\/strong>off<\/span><\/sub><\/strong>: \u89e3\u79bb<\/strong>\u901f\u7387<\/strong>\u5e38\u6570\u3002<\/strong>\u5982\u4e0b\u56fe\u6240\u793a\uff0c\u67d0\u6297\u4f53\u53ca\u5176\u7a81\u53d8\u4f53\u76f8\u5bf9\u4e8e\u67d0\u6297\u539f\u5728\u4e0d\u540cpH\u8fdb\u884c\u7ed3\u5408\u548c\u89e3\u79bb\u7684\u52a8\u529b\u5b66\u68c0\u6d4b\u7ed3\u679c<\/span>3<\/span><\/sup>\u3002\u52a8\u529b\u5b66\u68c0\u6d4b\u7684\u56fe\u5f62\u3002\u66f2\u7ebf\u5148\u5448\u4e0a\u5347\u8d8b\u52bf\uff0c\u8fd9\u4e2a\u662f\u7ed3\u5408\u66f2\u7ebf\uff1b\u7136\u540e\u4e3a\u4e0b\u964d\u8d8b\u52bf\uff0c\u4e3a\u89e3\u79bb\u66f2\u7ebf\u3002\u7ed3\u5408\u66f2\u7ebf\u7684\u659c\u7387\u53cd\u5e94\u4e86\u6297\u4f53\u548c\u6297\u539f\u7684\u53cd\u5e94\u901f\u5ea6\uff0c\u5728\u76f8\u540c\u7684\u4e0a\u6837\u6d53\u5ea6\u4e0b\uff0c\u4e5f\u8bf4\u660e\u7ed3\u5408\u901f\u7387\u5e38\u6570\u8d8a\u5927\uff1b\u89e3\u79bb\u66f2\u7ebf\u7684\u659c\u7387\u53cd\u5e94\u4e86\u6297\u4f53\u548c\u6297\u539f\u7684\u53cd\u5e94\u751f\u6210\u6297\u4f53-\u6297\u539f\u590d\u5408\u7269\u7684\u901f\u5ea6\uff0c\u5728\u76f8\u540c\u7684\u4e0a\u6837\u6d53\u5ea6\u4e0b\uff0c\u659c\u7387\u8d8a\u6296\u4e5f\u8bf4\u660e\u7ed3\u5408\u901f\u7387\u5e38\u6570\u8d8a\u5927\uff1b\u89e3\u79bb\u66f2\u7ebf\u7684\u659c\u7387\u53cd\u5e94\u4e86\u6297\u4f53-\u6297\u539f\u590d\u5408\u7269\u89e3\u79bb\u5f62\u6210\u6297\u4f53\u548c\u6297\u539f\u7684\u901f\u5ea6\uff0c\u5728\u76f8\u540c\u7684\u6297\u4f53-\u6297\u539f\u590d\u5408\u7269\u6d53\u5ea6\u4e0b\uff0c\u659c\u7387\u8d8a\u5e73\u7f13\u8bf4\u660e\u89e3\u79bb\u901f\u7387\u5e38\u6570\u8d8a\u5c0f\uff0c\u6839\u636e\u516c\u5f0f\u7ed3\u5408\u901f\u7387\u5e38\u6570\u8d8a\u5927\uff0c\u89e3\u79bb\u901f\u7387\u5e38\u6570\u8d8a\u5c0f\uff0c\u6297\u4f53\u76f8\u5bf9\u4e8e\u6297\u539f\u7684\u4eb2\u548c\u529b\u8d8a\u5f3a\u3002\u7c7b\u4f3c\u7684\u6280\u672f\u8fd8\u6709\u751f\u7269\u819c\u5e72\u6d89\u6280\u672f\u6d4b\u5b9a\u65b9\u6cd5\uff08BLI\uff09<\/span><\/p>\n

\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u6280\u672f\u4e4b\u4eb2\u548c\u529b\u8bc4\u4ef7\u7bc7\"<\/span><\/p>\n

<\/span><\/strong><\/h2>\n
\n
\n
\n
\u52a8\u6001\u5e73\u8861\u6d4b\u5b9a\u6cd5\uff08\u9971\u548c\u6d53\u5ea6\u6cd5\uff09<\/section>\n<\/section>\n<\/section>\n<\/section>\n

<\/span><\/strong><\/h2>\n

\u5982\u516c\u5f0f\u4e09\u6240\u793a\uff0c\u5982\u679c\u6211\u4eec\u9700\u8981\u6d4b\u91cfKD\uff0c\u6211\u4eec\u9700\u8981\u6d4b\u91cf\u52a8\u6001\u5e73\u8861\u4ee5\u540e\u6eb6\u6db2\u4e2d\u6e38\u79bb\u7684R\u7684\u6d53\u5ea6\uff0c\u6e38\u79bb\u7684L\u7684\u6d53\u5ea6\u548cR-L\u590d\u5408\u7269\u7684\u6d53\u5ea6\u3002\u8fd9\u65e0\u7591\u662f\u4e00\u4e2a\u590d\u6742\u7684\u68c0\u6d4b\u8fc7\u7a0b\uff0c\u5728\u5b9e\u9645\u7684\u5e94\u7528\u8fc7\u7a0b\u4e2d\u7ecf\u8fc7\u516c\u5f0f\u63a8\u5bfc\u548c\u5047\u8bbe\u4ee5\u540e\u8f6c\u53d8\u6210\u4ee5\u4e0b\u516c\u5f0f\uff0c\u5982\u4e0b\u56fe\u6240\u793a\u5373\u5c11\u91cfR\u5b58\u5728\u7684\u60c5\u51b5\u4e0b\uff0c\u5c06L\u8fdb\u884c\u68af\u5ea6\u7a00\u91ca\uff0c\u68c0\u6d4bR-L\u590d\u5408\u7269\u7684\u6d53\u5ea6\uff0c\u5f53R-L\u590d\u5408\u7269\u7684\u6d53\u5ea6\u5360\u603bR\u6d53\u5ea6\u7684\u4e00\u534a\u65f6\uff0cL\u5bf9\u5e94\u7684\u6d53\u5ea6\u503c\uff08EC50\uff09\u5373L\u76f8\u5bf9\u4e8eR\u7684KD\u503c<\/span>\u3002\u53ea\u6709\u5728\u6b64\u5728\u7279\u5b9a\u7684\u5b9e\u9a8c\u6761\u4ef6\u4e0bEC50\u503c\u80fd\u591f\u7b49\u4ef7\u4e8eKD\u503c\u3002\u52a8\u6001\u5e73\u8861\u6d4b\u5b9a\u6cd5\u6d4b\u5b9aKD\u503c\u6709\u51e0\u4e2a\u5173\u952e\u70b9\uff0c\u5373\u76f8\u4e92\u4f5c\u7528\u7684\u4e24\u4e2a\u5206\u5b50\uff0cR\u8981\u5c11\u91cf\uff0cL\u8981\u8fdb\u884c\u8fde\u7eed\u7684\u68af\u5ea6\u7a00\u91ca\uff0c\u5f97\u5230\u5e73\u53f0\u671f\u6700\u5927\u7684\u4fe1\u53f7\u54cd\u5e94\u503c\uff08\u6240\u6709\u7684R\u7684\u7ed3\u5408\u4f4d\u70b9\u88abL\u5360\u636e\uff09\uff0c\u90a3\u4e48\u5728\u6b64\u6761\u4ef6\u4e0b\uff0c\u5360\u636e\u4e00\u534aR\u65f6\uff0c\u6240\u9700\u8981\u7684L\u6d53\u5ea6\uff08EC50\uff09\u4e3aKD\u503c\u3002\u5229\u7528\u6b64\u65b9\u6cd5\u8fdb\u884cKD\u6d4b\u91cf\u7684\u65b9\u5f0f\u591a\u6837\uff0c\u5f80\u5f80\u4f1a\u5bf9L\u8fdb\u884c\u6807\u8bb0\uff0c\u7b49R-L\u5f62\u6210\u52a8\u6001\u5e73\u8861\u540e\uff0c\u5229\u7528\u7269\u7406\u624b\u6bb5\u53bb\u9664\u672a\u53cd\u5e94\u7684L\u540e\u6d4b\u5f97\u7684\u6807\u8bb0\u7684L\u7684\u4fe1\u53f7\u503c\u80fd\u591f\u5b8c\u5168\u4ee3\u8868R-L\u590d\u5408\u7269\u3002\u5177\u4f53\u7684\u5b9e\u9a8c\u65b9\u6cd5\u6709\u540c\u4f4d\u7d20\u6807\u8bb0\u6d4b\u5b9a\uff0c\u504f\u632f\u8367\u5149\u6d4b\u5b9a\uff0c\u6d41\u5f0f\u7ec6\u80de\u672f\u8fdb\u884c\u6d4b\u5b9a\uff0cELISA\u8fdb\u884c\u6d4b\u5b9a\u7b49\u591a\u79cd\u65b9\u6cd5\u3002\u8fd9\u6837\u7684\u6d4b\u91cf\u5bf9\u4e8eR\u7684\u6d53\u5ea6\u548c\u7eaf\u5ea6\u6ca1\u6709\u8981\u6c42\uff0c\u53ea\u8981\u76f8\u5bf9\u5c11\u91cf\uff0c\u5982\u6d41\u5f0f\u6d4b\u91cf\u65b9\u6cd5\u7684R\u5f80\u5f80\u662f\u7ec6\u80de\u643a\u5e26\u7684\uff0cCell-Based ELISA\u65b9\u6cd5\u4e2d\u53d7\u4f53\u7684\u63d0\u4f9b\u8005\u4e5f\u662f\u7ec6\u80de\uff0c\u4f46\u662f\u5bf9\u4e8eL\u662f\u9700\u8981\u6bd4\u8f83\u7eaf\u5ea6\u548c\u7cbe\u786e\u6d53\u5ea6\uff0c\u56e0\u5176\u6d53\u5ea6\u662f\u76f4\u63a5\u548cKD\u5bf9\u5e94\u7684\u3002<\/span><\/p>\n

\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u6280\u672f\u4e4b\u4eb2\u548c\u529b\u8bc4\u4ef7\u7bc7\"\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u6280\u672f\u4e4b\u4eb2\u548c\u529b\u8bc4\u4ef7\u7bc7\"<\/span><\/p>\n

<\/span><\/strong><\/h2>\n
\n
\n
\n
\u4e09\u7c7b\u68c0\u6d4b\u4eb2\u548c\u529b\u65b9\u6cd5\u6bd4\u8f83<\/section>\n<\/section>\n<\/section>\n<\/section>\n

<\/span><\/strong><\/h2>\n

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 <\/span><\/p>\n

\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u6280\u672f\u4e4b\u4eb2\u548c\u529b\u8bc4\u4ef7\u7bc7\"<\/p>\n


<\/p>\n

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<\/span><\/strong><\/h2>\n
\n
\n
\n
\u4eb2\u548c\u529b\u6d4b\u5b9a\u65b9\u6cd5\u4e4b\u7ade\u4e89\u6cd5<\/section>\n<\/section>\n<\/section>\n<\/section>\n

<\/span><\/strong><\/h2>\n

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<\/p>\n

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\n
\n
\n
\u5c55\u671b<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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\u53c2\u8003\u6587\u732e\uff1a<\/span><\/strong><\/p>\n

1. Enzymes: A Practical Introduction to Structure, Mechanism, and Data Analysis  Chapter.04 Protein-ligand binding equilibria  Robert A. Copeland<\/span><\/p>\n

2. Sarkar C A, Lowenhaupt K, Horan T, et al. Rational cytokine design for increased lifetime and enhanced potency using pH-activated “histidine switching”.[J]. Nature Biotechnology, 2002, 20(9):908.<\/span><\/p>\n

3. Igawa T, Ishii S, Tachibana T, et al. Antibody recycling by engineered pH-dependent antigen binding improves the duration of antigen neutralization[J]. Nature Biotechnology, 2010, 28(11):1203.<\/span><\/p>\n

4. Friguet B, Chaffotte A F, Djavadi-Ohaniance L, et al. Measurements of the true affinity constant in solution of antigen-antibody complexes by enzyme-linked immunosorbent assay[J]. Journal of Immunological Methods, 1985, 77(2):305-319.<\/span><\/p>\n

5. Youngnak P, Kozono Y, Kozono H, et al. Differential binding properties of B7-H1 and B7-DC to programmed death-1 [J]. Biochemical & Biophysical Research Communications, 2003, 307(3):672-677.<\/span><\/p>\n

6. Rudnick S I, Lou J, Shaller C C, et al. Influence of affinity and antigen internalization on the uptake and penetration of Anti-HER2 antibodies in solid tumors[J]. Cancer Research, 2011, 71(6):2250.<\/span><\/p>\n

7. Javier Chaparro-Riggers, Hong Liang, Rachel M. DeVay, Lanfang Bai, Janette E. Sutton, Wei Chen, Tao Geng, Kevin Lindquist, Meritxell Galindo Casas, Leila M. Boustany, Colleen L. Brown, Jeffrey Chabot, Bruce Gomes, Pamela Garzone, Andrea Rossi, Pavel Strop, Dave Shelton, Jaume Pons, Arvind Rajpal. Increasing serum half-life and extending cholesterol lowering in vivo by engineering antibody with pH-sensitive binding to PCSK9.[J]. Journal of Biological Chemistry, 2012, 287(14):11090-7.<\/span><\/p>\n

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