{"id":19362,"date":"2018-07-12T01:00:00","date_gmt":"2018-08-08T01:10:14","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/2018\/08\/08\/%e7%94%9f%e7%89%a9%e5%ad%a6%e5%8a%9f%e8%83%bd%e5%ad%a6%e7%ad%9b%e9%80%89%e8%af%84%e4%bb%b7%e4%b9%8b%e9%85%b6%e5%ad%a6%e7%ad%9b%e9%80%89%e8%af%84%e4%bb%b7%e7%af%87\/"},"modified":"2018-08-08T09:10:18","modified_gmt":"2018-08-08T01:10:18","slug":"%e7%94%9f%e7%89%a9%e5%ad%a6%e5%8a%9f%e8%83%bd%e5%ad%a6%e7%ad%9b%e9%80%89%e8%af%84%e4%bb%b7%e4%b9%8b%e9%85%b6%e5%ad%a6%e7%ad%9b%e9%80%89%e8%af%84%e4%bb%b7%e7%af%87","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/19362\/","title":{"rendered":"\u751f\u7269\u5b66\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u9176\u5b66\u7b5b\u9009\u8bc4\u4ef7\u7bc7"},"content":{"rendered":"
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\u6458\u8981<\/section>\n<\/section>\n<\/section>\n<\/section>\n

      \u5728\u7edd\u5927\u6570\u836f\u7269\u9776\u70b9\u4e2d\u9176\u5b66\u9776\u70b9\u662f\u4e00\u4e2a\u5927\u7c7b\u3002\u672c\u6587\u4e3b\u8981\u4ee5\u9176\u50ac\u5316\u7684\u57fa\u672c\u5316\u5b66\u53cd\u5e94\u539f\u7406\u4e3a\u8d77\u70b9\uff0c\u6982\u8ff0\u548c\u9610\u8ff0\u4e0b\u9176\u50ac\u5316\u53cd\u5e94\u7684\u51e0\u4e2a\u57fa\u672c\u6982\u5ff5Km\uff0cKcat\u548cKi\uff0c\u4ee5\u53ca\u5176\u6d4b\u5b9a\u65b9\u6cd5\uff0c\u5206\u6790\u4e0b\u4e00\u4e2a\u7075\u654f\u7684\u9176\u5b66\u7b5b\u9009\u65b9\u6cd5\u5f00\u53d1\u7684\u51e0\u4e2a\u5173\u952e\u6b65\u9aa4\uff0c\u6982\u62ec\u8bba\u8ff0\u4e0b\u9176\u6291\u5236\u5242\u7684\u7c7b\u578b\u4ee5\u53ca\u5728\u5b9e\u9a8c\u68c0\u6d4b\u8fc7\u7a0b\u4e2d\u4e0d\u540c\u7c7b\u578b\u9176\u6291\u5236\u5242\u7684IC50\u548cKi\u4e4b\u95f4\u7684\u5173\u8054\u3002<\/span><\/p>\n

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<\/span><\/h2>\n
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3. \u9009\u53d6\u68af\u5ea6\u6d53\u5ea6\u7a00\u91ca\u7684\u5e95\u7269\u52a8\u529b\u5b66\u68c0\u6d4b\u7684\u7ebf\u6027\u533a\u57df\u7684\u659c\u7387\u4f5c\u4e3a\u521d\u59cb\u53cd\u5e94\u901f\u5ea6V0<\/span><\/sub>\u8fdb\u884c\u6570\u636e\u7c73\u6c0f\u65b9\u7a0b\u62df\u5408\uff0c\u62df\u5408\u56fe\u5f62\u7684\u5e73\u53f0\u671f\u4e3aVmax<\/span><\/sub>, 1\/2\u7684Vmax<\/span><\/sub>\u65f6V0<\/span><\/sub>\u5bf9\u5e94\u7684\u5e95\u7269\u6d53\u5ea6\u503c\u662fKm\u503c\u3002\u5904\u7406\u7ed3\u679c\u793a\u610f\u56fe\u5982\u4e0b\uff1a<\/span><\/p>\n

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4. \u5f53\u9176\u50ac\u5316\u53cd\u5e94\u8fbe\u5230\u6700\u5927\u901f\u5ea6\u65f6\uff0cS\u8fdc\u8fdc\u5927\u4e8eE\uff0c\u6240\u4ee5\u6240\u6709\u7684E\u5747\u88abS\u6355\u83b7\uff0cE\u548cS\u7684\u9006\u53cd\u5e94\u901f\u5ea6\u53ef\u5ffd\u7565\u4e0d\u8ba1\uff0c[ES]=[E]<\/span><\/p>\n

Vmax<\/span><\/sub>=[ES]*Kcat<\/span><\/sub>=[E] *Kcat<\/span><\/sub>,\u6240\u4ee5K<\/strong>cat<\/span><\/sub><\/strong>= V<\/strong>max<\/span><\/sub><\/strong>\/[E]<\/strong><\/span><\/p>\n

\u4ee5\u4e0a\u662fKm\u548cKcat\u7684\u6d4b\u5b9a\u548c\u8ba1\u7b97\u8fc7\u7a0b\uff0c\u503c\u5f97\u6ce8\u610f\u7684\u662f\u5728\u5b9e\u9645\u7684\u5b9e\u9a8c\u8fc7\u7a0b\u4e2d\u9700\u8981\u6ce8\u610f\u4ee5\u4e0b\u51e0\u70b9\uff1a<\/span><\/p>\n

1. \u5fc5\u987b\u662f\u52a8\u529b\u5b66\u8bfb\u6570\uff0c\u9009\u53d6\u521d\u59cb\u53cd\u6620\u7684\u7ebf\u6027\u6bb5\u7684\u659c\u7387\u4f5c\u4e3a\u521d\u59cb\u53cd\u5e94\u7684\u901f\u5ea6\u3002\u7ec8\u70b9\u6cd5\u8bfb\u6570\u7684\u7ed3\u679c\u5f88\u591a\u65f6\u5019\u5e76\u4e0d\u53ef\u9760\uff0c\u5e94\u4e3a\u9176\u50ac\u5316\u53cd\u5e94\u968f\u7740\u65f6\u95f4\u7684\u8fdb\u884c\u5e95\u7269\u7684\u6d88\u8017\uff0c\u53cd\u5e94\u901f\u5ea6\u4f1a\u4ece\u5300\u901f\u5230\u975e\u5747\u901f\u53d8\u5316\uff0c\u5982\u679c\u91c7\u7528\u7ec8\u70b9\u6cd5\u53ef\u80fd\u5f97\u5230\u4e0d\u771f\u5b9e\u7684\u521d\u59cb\u901f\u5ea6\uff0c\u5982\u4e0b\u56fe\uff1a<\/span><\/p>\n

\"\u751f\u7269\u5b66\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u9176\u5b66\u7b5b\u9009\u8bc4\u4ef7\u7bc7\"<\/p>\n


<\/span><\/p>\n

2. \u5fc5\u987b\u786e\u8ba4\u4eea\u5668\u7684\u68c0\u6d4b\u7ebf\u6027\uff0c\u5f53\u9176\u50ac\u5316\u53cd\u5e94\uff0c\u4ea7\u7269\u7684\u91cf\u8fc7\u591a\u65f6\uff0c\u8d85\u8fc7\u4eea\u5668\u68c0\u6d4b\u7684\u7ebf\u6027\u533a\u57df\u65f6\uff0c\u68c0\u6d4b\u7684\u4ea7\u7269\u7684\u91cf\u4e0d\u51c6\uff0c\u5f71\u54cd\u5230\u540e\u7eed\u53c2\u6570\u7684\u8ba1\u7b97\uff0c\u5982\u4e0b\u56fe\uff1a<\/span><\/p>\n

\"\u751f\u7269\u5b66\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u9176\u5b66\u7b5b\u9009\u8bc4\u4ef7\u7bc7\"<\/p>\n

<\/span><\/p>\n

<\/span><\/h2>\n
\n
\n
\n
\u9176\u5b66\u836f\u7269\u7b5b\u9009\u6a21\u578b\u7684\u5efa\u7acb<\/section>\n<\/section>\n<\/section>\n<\/section>\n

<\/span><\/h2>\n

\u9176\u5b66\u7b5b\u9009\u65b9\u6cd5\u7684\u51e0\u4e2a\u6838\u5fc3\u53c2\u6570\u5305\u62ec\u9176\uff0c\u53cd\u5e94\u73af\u5883\uff0c\u5e95\u7269\u548c\u6291\u5236\u7269\u3002\u4e00\u822c\u6765\u8bb2\u53cd\u5e94\u73af\u5883\u9700\u8981\u67e5\u9605\u6587\u732e\u8fdb\u884c\u8c03\u7814\uff0c\u786e\u5b9aPH\u503c\uff0c\u79bb\u5b50\u5f3a\u5ea6\uff0c\u6d17\u6da4\u5242\u7b49\u5bf9\u9176\u6d3b\u7684\u5f71\u54cd\uff0c\u5e95\u7269\u53ef\u4ee5\u6839\u636e\u9176\u50ac\u5316\u539f\u7406\u9009\u62e9\u5929\u7136\u5e95\u7269\uff0c\u4e5f\u53ef\u4ee5\u9009\u62e9\u5408\u6210\u5e95\u7269\uff08\u5e95\u7269\u7684\u9009\u62e9\u548c\u540e\u7eed\u914d\u5957\u7684\u68c0\u6d4b\u65b9\u6cd5\u7d27\u5bc6\u76f8\u5173\uff09\u3002\u4e00\u4e2a\u9176\u5b66\u65b9\u6cd5\u7684\u5f00\u53d1\u53ef\u80fd\u6d89\u53ca\u5230\u4ee5\u4e0b\u4e00\u4e9b\u56e0\u7d20\uff1a<\/span><\/p>\n

\u5e95\u7269\u7684\u9009\u62e9\u548c\u914d\u5957\u7684\u68c0\u6d4b\u65b9\u5f0f\uff0c\u5982\u4e0b\u56fe\u67d0\u86cb\u767d\u6c34\u89e3\u9176\uff0c\u5176\u8bc6\u522b\u7684\u4f4d\u70b9\u662f\u6c28\u57fa\u9178\u7ebf\u6027\u8868\u4f4d\uff0c\u53ef\u4ee5\u4eba\u5de5\u5408\u6210\u591a\u80bd\uff0c\u5728\u5176N\u7aef\u548cC\u7aef\u5206\u522b\u52a0\u5165\u8367\u5149\u57fa\u56e2\u548c\u6dec\u706d\u57fa\u56e2\uff0c\u6b63\u5e38\u72b6\u6001\u4e0b\u6216\u8005\u9176\u6d3b\u6291\u5236\u72b6\u6001\uff0c\u8be5\u591a\u80bd\u5728\u9176\u6807\u4eea340nm\u6fc0\u53d1\u65f6\uff0c\u5176\u53d1\u5c04\u5149\u4f1a\u88ab\u6dec\u706d\u57fa\u56e2\u5438\u6536\uff0c\u5728405nm\u65e0\u4fe1\u53f7\u4ea7\u751f\uff1b\u9176\u50ac\u5316\u53cd\u5e94\u4f1a\u5c06\u8be5\u591a\u80bd\u526a\u5207\uff0c\u5f53\u9176\u6807\u4eea340nm\u6fc0\u53d1\u65f6\uff0c405nm\u6709\u4fe1\u53f7\u4ea7\u751f\u3002<\/span><\/p>\n

\"\u751f\u7269\u5b66\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u9176\u5b66\u7b5b\u9009\u8bc4\u4ef7\u7bc7\"<\/p>\n

<\/span><\/p>\n

\u53c8\u5982\u67d0\u75c5\u6bd2\u9006\u8f6c\u5f55\u9176\uff0c\u5408\u6210RNA\u6a21\u677f\u548c\u5bf9\u5e94\u5f15\u7269\uff0c\u5728\u5176\u5f15\u7269\u4e0a\u8fdb\u884c\u751f\u7269\u7d20\u6807\u8bb0\uff0c\u5176\u4e2d\u78b1\u57fa\u4e0a\u8fdb\u884cRu\u6807\u8bb0\uff0c\u968f\u7740\u9176\u50ac\u5316\u7684\u9006\u8f6c\u5f55\u53cd\u5e94\u7684\u8fdb\u884c\uff0c\u5f15\u7269\u4e0a\u4e0d\u65ad\u6709Ru\u6807\u8bb0\u78b1\u57fa\u4e0a\u53bb\u5f62\u6210\u6838\u82f7\u9178\u5e8f\u5217\uff0c\u901a\u8fc7\u4eb2\u548c\u7d20\u7684\u677f\u5b50\u6355\u6349\u5f15\u7269\uff0c\u68c0\u6d4bRu\u4fe1\u53f7\u503c\u6765\u8bc4\u5224\u9176\u7684\u50ac\u5316\u548c\u6291\u5236\u7684\u6d3b\u6027\u3002<\/span><\/p>\n

\"\u751f\u7269\u5b66\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u9176\u5b66\u7b5b\u9009\u8bc4\u4ef7\u7bc7\"<\/p>\n


<\/span><\/p>\n

\u9176\uff0c\u5e95\u7269\u6d53\u5ea6\u7684\u9009\u62e9\u548c\u53cd\u5e94\u65f6\u95f4\u7684\u9009\u62e9<\/strong><\/span><\/p>\n

\u5f53\u9176\u5bf9\u5e94\u7684\u5e95\u7269\u786e\u5b9a\u4ee5\u540e\uff0c\u9009\u53d6\u5c11\u91cf\u7684\u9176\u6d53\u5ea6\uff08pM\u6216\u8005nM\uff09\u5bf9\u5e95\u7269\u8fdb\u884cKm\u6d4b\u5b9a\uff0c\u9009\u53d6\u5e95\u7269\u7684Km\u6d53\u5ea6\u4f5c\u4e3a\u7b5b\u9009\u7684\u53cd\u5e94\u6d53\u5ea6\uff0c\u9009\u53d6\u8be5\u6d53\u5ea6\u4e0b\u7ebf\u6027\u7684\u53cd\u5e94\u65f6\u95f4\u5185\u7684\u67d0\u4e2a\u65f6\u95f4\u70b9\u4f5c\u4e3a\u53cd\u5e94\u7ec8\u6b62\u6216\u8005\u68c0\u6d4b\u7684\u65f6\u95f4\u3002\u9176\u548c\u68af\u5ea6\u7a00\u91ca\u7684\u5316\u5408\u7269\u8fdb\u884c\u9884\u5b75\u80b2\u540e\uff0c\u52a0\u5165Km\u6d53\u5ea6\u7684\u5e95\u7269\uff0c\u53cd\u5e94\u4e00\u6bb5\u65f6\u95f4\u540e\u8fdb\u884c\u68c0\u6d4b\uff0c\u8fd9\u4e2a\u5c31\u662f\u4e00\u822c\u9176\u5b66\u7b5b\u9009\u6a21\u578b\u3002<\/span> <\/span><\/p>\n

\"\u751f\u7269\u5b66\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u9176\u5b66\u7b5b\u9009\u8bc4\u4ef7\u7bc7\"<\/p>\n

<\/span><\/h2>\n
\n
\n
\n
\u9176\u6291\u5236\u5242\u673a\u7406\u7814\u7a76<\/section>\n<\/section>\n<\/section>\n<\/section>\n

<\/span><\/h2>\n

\u5bf9\u4e8e\u9776\u70b9\u9176\u7684\u6291\u5236\u5242\uff0c\u4e3b\u8981\u53ef\u4ee5\u5f52\u7eb3\u4e3a\u4e09\u7c7b\uff08competitive\u7ade\u4e89\u6027, noncompetitive\u975e\u7ade\u4e89\u6027, uncompetitive\u4e0d\u7ade\u4e89\u6027\uff09<\/span><\/p>\n

competitive\u7ade\u4e89\u6027\u6291\u5236\u5242\u53ea\u548c\u81ea\u7531\u7684\u9176\u7ed3\u5408\uff0c\u901a\u5e38\u60c5\u51b5\u4e0b\u548c\u5e95\u7269\u5171\u540c\u7ade\u4e89\u7ed3\u5408\u4f4d\u70b9\uff08\u6d3b\u6027\u4e2d\u5fc3\uff09\uff0c\u5f53\u7136\u4e5f\u6709\u7ade\u4e89\u6027\u6291\u5236\u5242\u548c\u5e95\u7269\u4e92\u65a5\u7684\u60c5\u51b5\u51fa\u73b0\uff0c\u4e24\u4e2a\u4e2d\u53ea\u6709\u4e00\u4e2a\u80fd\u591f\u548c\u81ea\u7531\u7684\u9176\u7ed3\u5408\u3002\u5728\u7ade\u4e89\u6027\u6291\u5236\u5242\u5b58\u5728\u7684\u60c5\u51b5\u4e0b\uff0c\u9176\u76f8\u5bf9\u4e8e\u5e95\u7269\u7684Km\u548cVmax\u6d4b\u5b9a\u65f6\uff0c\u6d4b\u5f97\u5f97\u8868\u89c2Km\u503c\u589e\u52a0\uff0cVmax\u503c\u4e0d\u53d8\uff0c\u5982\u4e0b\u56fe\u6240\u793a\uff0c\u51e0\u79cd\u7ade\u4e89\u6027\u6291\u5236\u5242\u7684\u56fe\u793a\uff1a<\/span><\/p>\n

\"\u751f\u7269\u5b66\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u9176\u5b66\u7b5b\u9009\u8bc4\u4ef7\u7bc7\"<\/p>\n

<\/span><\/p>\n

noncompetitive\u975e\u7ade\u4e89\u6027\u6291\u5236\u5242<\/span><\/p>\n

\u975e\u7ade\u4e89\u6027\u6291\u5236\u5242\u53ef\u4ee5\u548c\u81ea\u7531\u7684\u9176\u7ed3\u5408\uff0c\u4e5f\u53ef\u4ee5\u548c\u9176-\u5e95\u7269\u590d\u5408\u7269\u7ed3\u5408\uff0c\u6291\u5236\u5242\u7ed3\u5408\u7684\u4f4d\u70b9\u548c\u6d3b\u6027\u4e2d\u5fc3\u4f4d\u70b9\u4e0d\u5728\u76f8\u540c\u7684\u8868\u4f4d\uff0c\u6700\u7ec8\u4e5f\u4f1a\u5bfc\u81f4\u9176\u50ac\u5316\u6d3b\u6027\u7684\u4e27\u5931\u3002\u5728\u975e\u7ade\u4e89\u6027\u6291\u5236\u5242\u5b58\u5728\u7684\u60c5\u51b5\u4e0b\uff0c\u9176\u76f8\u5bf9\u4e8e\u5e95\u7269\u7684Km\u548cVmax\u6d4b\u5b9a\u65f6\uff0c\u6d4b\u5f97\u5f97\u8868\u89c2Km\u503c\u4e0d\u53d8\uff0cVmax\u503c\u964d\u4f4e\uff0c\u5982\u4e0b\u56fe\u6240\u793a\uff1a<\/span><\/p>\n

\"\u751f\u7269\u5b66\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u9176\u5b66\u7b5b\u9009\u8bc4\u4ef7\u7bc7\" <\/span><\/p>\n

uncompetitive\u4e0d\u7ade\u4e89\u6027\u6291\u5236\u5242<\/span><\/p>\n

\u4e0d\u7ade\u4e89\u6027\u6291\u5236\u5242\u53ea\u548c\u9176-\u5e95\u7269\u590d\u5408\u7269\u7ed3\u5408\uff0c\u5f62\u6210\u5931\u6d3b\u7684\u6291\u5236\u5242-\u9176-\u5e95\u7269\u590d\u5408\u7269\u3002\u9176\u76f8\u5bf9\u4e8e\u5e95\u7269\u7684Km\u548cVmax\u6d4b\u5b9a\u65f6\uff0c\u6d4b\u5f97\u5f97\u8868\u89c2Km\u503c\u964d\u4f4e\uff0cVmax\u503c\u964d\u4f4e\u3002\u5982\u4e0b\u56fe\u6240\u793a\uff1a<\/span><\/p>\n

\"\u751f\u7269\u5b66\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u9176\u5b66\u7b5b\u9009\u8bc4\u4ef7\u7bc7\"<\/p>\n

<\/span><\/p>\n

<\/span><\/h2>\n
\n
\n
\n
\u4e0d\u540c\u7c7b\u578b\u6291\u5236\u5242Ki\u548cIC50\u4e4b\u95f4\u7684\u5173\u8054<\/section>\n<\/section>\n<\/section>\n<\/section>\n

<\/span><\/h2>\n

Ki\u63cf\u8ff0\u7684\u662f\u6291\u5236\u5242\u548c\u9176\u7684\u7ed3\u5408\u5f3a\u5f31\uff0c\u662f\u6291\u5236\u5242\u548c\u9176\u7684\u89e3\u79bb\u5e73\u8861\u5e38\u6570\uff0c\u662f\u4e2a\u72b6\u6001\u51fd\u6570\uff0c\u4e0d\u4f1a\u53d7\u5230\u53cd\u5e94\u4f53\u7cfb\u4e2d\u5e95\u7269\u6d53\u5ea6\u7684\u5f71\u54cd\uff0c\u4e0d\u540c\u5b9e\u9a8c\u5f97\u5230\u7684Ki\u503c\u5177\u6709\u53ef\u6bd4\u6027\uff1b\u800cIC50\u5219\u662f\u5728\u7279\u5b9a\u9176\u5b66\u68c0\u6d4b\u65b9\u6cd5\u4e2d\u9176\u6d3b\u6291\u5236\u4e00\u534a\u65f6\u6291\u5236\u5242\u7684\u6d53\u5ea6\uff0c\u6839\u636e\u4e0d\u540c\u7c7b\u578b\u7684\u6291\u5236\u5242\uff0c\u5e95\u7269\u6d53\u5ea6\u53ef\u80fd\u5bf9IC50\u4ea7\u751f\u5f71\u54cd\uff0c\u4e0d\u540c\u5b9e\u9a8c\u5f97\u5230\u7684IC50\u503c\u4e0d\u5177\u53ef\u6bd4\u6027\u3002\u6839\u636e\u4e0d\u540c\u7c7b\u578b\u7684\u6291\u5236\u5242\uff0c\u5728\u7406\u60f3\u6761\u4ef6\u4e0b\uff08\u9176\u6d53\u5ea6\u8fdc\u8fdc\u5c0f\u4e8e\u5e95\u7269\u6d53\u5ea6\u8fdb\u884c\u53cd\u5e94\uff09\uff0cKi\u548cIC50\u7684\u5173\u7cfb\u5982\u4e0b\uff1a<\/span><\/p>\n

\"\u751f\u7269\u5b66\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u9176\u5b66\u7b5b\u9009\u8bc4\u4ef7\u7bc7\"<\/p>\n

\u4e0d\u540c\u6291\u5236\u5242\u7c7b\u578bIC50\u548c\u53cd\u5e94\u4f53\u7cfb\u4e2d\u52a0\u5165\u7684\u5e95\u7269\u6d53\u5ea6\u7684\u5173\u7cfb\u793a\u610f\u56fe\u5982\u4e0b\uff1a<\/span><\/p>\n

\"\u751f\u7269\u5b66\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u9176\u5b66\u7b5b\u9009\u8bc4\u4ef7\u7bc7\"<\/p>\n

<\/span><\/p>\n

\n
\n
\n
\u5c55\u671b\uff1a<\/section>\n<\/section>\n<\/section>\n<\/section>\n

<\/span><\/h2>\n

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    \u5229\u7528\u9176\u50ac\u5316\u53cd\u5e94\u8fdb\u884c\u4f53\u5916\u7684\u529f\u80fd\u5b66\u68c0\u6d4b\u4e5f\u662f\u5e38\u7528\u7684\u5de5\u5177\uff0c\u5982ELISA\u4e2dHRP\u9176\u7684\u50ac\u5316\u663e\u8272\uff0c\u7ec6\u80de\u6d3b\u6027\u68c0\u6d4b\u65f6\u7425\u73c0\u9178\u8131\u6c22\u9176\u50ac\u5316\u7684C<\/span>CK8\u68c0\u6d4b\uff0c\u62a5\u544a\u57fa\u56e0\u68c0\u6d4b\u8fc7\u7a0b\u4e2dluciferase\u9176\u50ac\u5316\u7684\u5316\u5b66\u53d1\u5149\u7b49\uff0c\u4e86\u89e3\u5176\u5316\u5b66\u539f\u7406\u548c\u52a8\u529b\u5b66\u53cd\u5e94\u8fdb\u7a0b\u6709\u52a9\u4e8e\u529f\u80fd\u5b66\u65b9\u6cd5\u5b66\u5f00\u53d1\uff0c\u4f18\u5316\u68c0\u6d4b\u65b9\u6cd5\u7684\u6761\u4ef6\u3002<\/span><\/p>\n

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\u53c2\u8003\u8d44\u6599<\/strong><\/span><\/h2>\n

\u300aGuidance for Assay Development & HTS<\/strong> \u300b<\/strong><\/span><\/p>\n

SECTION V: ENZYMATIC ASSAYS<\/strong><\/span><\/p>\n

SECTION XII: MECHANISM OF ACTION ASSAYS<\/strong> <\/strong>FOR ENZYMES<\/strong><\/span><\/p>\n

\u8bc6\u522b\u5fae\u4fe1\u4e8c\u7ef4\u7801\uff0c\u6dfb\u52a0\u751f\u7269\u5236\u54c1\u5708\u5c0f\u7f16\uff0c\u7b26\u5408\u6761\u4ef6\u8005\u5373\u53ef\u52a0\u5165\u751f\u7269\u5236\u54c1\u5708\u5fae\u4fe1\u7fa4\uff01<\/span><\/strong>
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\u8bf7\u6ce8\u660e\uff1a\u59d3\u540d+\u7814\u7a76\u65b9\u5411\uff01<\/span><\/strong><\/span><\/p>\n

\n

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\u59cb\u53d1\u4e8e\u5fae\u4fe1\u516c\u4f17\u53f7\uff1a \u751f\u7269\u5236\u54c1\u5708 <\/p>\n<\/blockquote>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

\u6458\u8981       \u5728\u7edd\u5927\u6570\u836f\u7269\u9776\u70b9\u4e2d\u9176\u5b66\u9776\u70b9\u662f\u4e00\u4e2a\u5927\u7c7b\u3002\u672c\u6587\u4e3b\u8981\u4ee5\u9176\u50ac\u5316\u7684\u57fa\u672c\u5316 […]<\/p>\n","protected":false},"author":1,"featured_media":19363,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[2],"tags":[],"_links":{"self":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/19362"}],"collection":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/comments?post=19362"}],"version-history":[{"count":1,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/19362\/revisions"}],"predecessor-version":[{"id":19384,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/19362\/revisions\/19384"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media\/19363"}],"wp:attachment":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media?parent=19362"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/categories?post=19362"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/tags?post=19362"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}