{"id":19385,"date":"2018-07-12T01:00:00","date_gmt":"2018-08-08T01:10:20","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/2018\/08\/08\/%e7%94%9f%e7%89%a9%e5%8a%9f%e8%83%bd%e5%ad%a6%e7%ad%9b%e9%80%89%e8%af%84%e4%bb%b7%e4%b9%8b%e7%bb%86%e8%83%9e%e5%8a%9f%e8%83%bd%e5%ad%a6%e8%af%84%e4%bb%b7%e7%af%87\/"},"modified":"2018-08-08T09:10:23","modified_gmt":"2018-08-08T01:10:23","slug":"%e7%94%9f%e7%89%a9%e5%8a%9f%e8%83%bd%e5%ad%a6%e7%ad%9b%e9%80%89%e8%af%84%e4%bb%b7%e4%b9%8b%e7%bb%86%e8%83%9e%e5%8a%9f%e8%83%bd%e5%ad%a6%e8%af%84%e4%bb%b7%e7%af%87","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/19385\/","title":{"rendered":"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u7ec6\u80de\u529f\u80fd\u5b66\u8bc4\u4ef7\u7bc7"},"content":{"rendered":"<div class=\"bpp-post-content\">\n<section class=\"\"   style=\"max-width: 100%;box-sizing: border-box;color: rgb(51, 51, 51);\">\n<section style=\"margin-top: 25px;padding: 5px;max-width: 100%;box-sizing: border-box;border-width: 1px;border-style: solid;border-color: rgb(204, 204, 204);line-height: 24px;color: inherit;word-wrap: break-word !important;\">\n<section style=\"margin-top: -20px;margin-left: 15px;max-width: 100%;box-sizing: border-box;width: auto;color: inherit;word-wrap: break-word !important;\"><span style=\"max-width: 100%;color: inherit;box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"margin-top: 15px;margin-right: 8px;max-width: 100%;color: rgb(102, 102, 102);box-sizing: border-box !important;word-wrap: break-word !important;\"><span class=\"\"  style=\"padding: 4px 10px;max-width: 100%;border-color: rgb(89, 155, 171);border-radius: 5px;color: rgb(255, 255, 255);background-color: rgb(89, 155, 171);box-sizing: border-box !important;word-wrap: break-word !important;\">\u6458\u8981\uff1a<\/span>&nbsp;&nbsp;<\/strong><span class=\"\"  style=\"max-width: 100%;border-color: rgb(89, 155, 171);color: rgb(89, 155, 171);background-color: 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style=\"max-width: 100%;min-height: 1em;text-indent: 0em;box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 15px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u5728\u7ebf\u57f9\u8bad\u63a8\u8350\uff1a<\/span><\/strong><span style=\"max-width: 100%;font-size: 15px;box-sizing: border-box !important;word-wrap: break-word !important;\"><a href=\"https:\/\/mp.weixin.qq.com\/s?__biz=MjM5NTI3MDE0NQ==&amp;mid=2653619317&amp;idx=1&amp;sn=dc20b928fd908a4339a2d4fea4d4e077&amp;scene=21#wechat_redirect\" target=\"_blank\" style=\"color: rgb(87, 107, 149);-webkit-tap-highlight-color: rgba(0, 0, 0, 0);max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\u5728\u7ebf\u4ea4\u6d41|\u4f53\u5916\u751f\u7269\u5b66\u65b9\u6cd5\u5f00\u53d1-\u57fa\u672c\u539f\u7406\u548c\u5b9e\u8df5<\/a><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<h2 style=\"font-weight: bold;max-width: 100%;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><\/h2>\n<h2 style=\"font-weight: bold;max-width: 100%;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><\/h2>\n<section class=\"\"   style=\"max-width: 100%;box-sizing: border-box;color: rgb(51, 51, 51);\">\n<section class=\"\"  placeholder=\"\u6df1\u8272\u8fb9\u6846\u6807\u9898\" style=\"margin-top: 10px;margin-bottom: 10px;padding: 10px;max-width: 100%;box-sizing: border-box;line-height: 25px;color: rgb(110, 156, 40);border-radius: 4px;box-shadow: rgb(153, 153, 153) 2px 2px 4px;border-left: 10px solid rgb(184, 222, 127);border-top-color: rgb(184, 222, 127);border-right-color: rgb(184, 222, 127);border-bottom-color: rgb(184, 222, 127);background-color: rgb(229, 243, 208);word-wrap: break-word !important;\">\u7ec6\u80de\u7684\u751f\u7406\u53cd\u5e94\u8fdb\u7a0b\u6982\u8ff0<\/section>\n<\/section>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;text-indent: 28px;white-space: pre-wrap;line-height: inherit;box-sizing: border-box !important;word-wrap: break-word !important;\">\u7ec6\u80de\u662f\u751f\u547d\u6d3b\u52a8\u7684\u57fa\u672c\u5355\u4f4d\uff0c\u4ee5\u7ec6\u80de\u4e3a\u7814\u7a76\u5bf9\u8c61\u7684\u529f\u80fd\u5b66\u8bc4\u4ef7\u65b9\u6cd5\u6709\u522b\u4e8e\u5728\u4f53\u5916\u7406\u60f3\u6761\u4ef6\u4e0b\u7684\u751f\u7269\u5316\u5b66\u53cd\u5e94\u8fc7\u7a0b\uff08\u86cb\u767d\u95f4\u76f8\u4e92\u4f5c\u7528\uff0c\u9176\u50ac\u5316\u53cd\u5e94\uff09\u7b49\uff0c\u5176\u751f\u7406\u53cd\u5e94\u8fdb\u7a0b\u4e3a\u591a\u9636\u6bb5\u7684\uff0c\u53ef\u80fd\u6d89\u53ca\u5230\u591a\u4e2a\u53d7\u4f53\u86cb\u767d<\/span><span style=\"max-width: 100%;font-size: 14px;text-indent: 28px;white-space: pre-wrap;line-height: inherit;box-sizing: border-box !important;word-wrap: break-word !important;\">\/<\/span><span style=\"max-width: 100%;font-size: 14px;text-indent: 28px;white-space: pre-wrap;line-height: inherit;box-sizing: border-box !important;word-wrap: break-word !important;\">\u4fe1\u53f7\u901a\u8def\uff0c\u534f\u540c<\/span><span style=\"max-width: 100%;font-size: 14px;text-indent: 28px;white-space: pre-wrap;line-height: inherit;box-sizing: border-box !important;word-wrap: break-word !important;\">\/<\/span><span style=\"max-width: 100%;font-size: 14px;text-indent: 28px;white-space: pre-wrap;line-height: inherit;box-sizing: border-box !important;word-wrap: break-word !important;\">\u62ee\u6297\u7684\u590d\u6742\u8fc7\u7a0b\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u7ec6\u80de\u5355\u4e00\u7684\u751f\u7406\u53cd\u5e94\u8fdb\u7a0b\uff0c\u5982\u4e0b\u56fe\u6a21\u578b\u6240\u793a\uff0c\u53ef\u5206\u4e3a\u56db\u4e2a\u65b9\u9762\uff1a<\/span><\/p>\n<ol class=\"list-paddingleft-2\" style=\"\">\n<li>\n<p style=\"max-width: 100%;min-height: 1em;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: 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1em;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u653e\u5927\u7684\u4fe1\u53f7\u7ee7\u7eed\u4f20\u9012\u5f71\u54cd\u5230\u4e0b\u6e38\u7684\u8f6c\u5bfc\u4fe1\u53f7\u901a\u8def\uff0c\u5f71\u54cd\u5230\u8f6c\u5f55\u56e0\u5b50\u7684\u6d3b\u6027\uff0c\u8f6c\u5f55\u56e0\u5b50\u8fdb\u5165\u7ec6\u80de\u6838\uff0c\u4ecb\u5bfc\u6838\u82f7\u9178\u7684\u8f6c\u5f55\uff0c\u8f6c\u5f55RNA\u8fdb\u5165\u7ec6\u80de\u57fa\u8d28\u4e2d\u8c03\u8282\u86cb\u767d\u7684\u8868\u8fbe\u3002<\/span><\/p>\n<\/li>\n<li>\n<p style=\"max-width: 100%;min-height: 1em;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;line-height: 22.4px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u86cb\u767d\u7684\u4e30\u5ea6\u8868\u8fbe\u5bfc\u81f4\u7ec6\u80de\u5bf9\u4fe1\u53f7\u5206\u5b50\u4ea7\u751f\u6700\u7ec8\u7684\u54cd\u5e94\uff0c\u5f15\u8d77\u7ec6\u80de\u5411\u5916\u754c\u5206\u6ccc\u7269\u8d28\uff0c\u7ec6\u80de\u589e\u6b96\u6216\u8005\u6b7b\u4ea1\u3002<\/span><\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;box-sizing: border-box !important;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;text-align: center;box-sizing: border-box !important;word-wrap: break-word !important;\"><img class=\"\"      style=\"box-sizing: border-box !important;word-wrap: break-word !important;visibility: visible !important;width: auto !important;\" width=\"auto\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/08\/beepress7-1533690620.jpg\" alt=\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u7ec6\u80de\u529f\u80fd\u5b66\u8bc4\u4ef7\u7bc7\" title=\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u7ec6\u80de\u529f\u80fd\u5b66\u8bc4\u4ef7\u7bc7\"  \/><\/p>\n<\/li>\n<\/ol>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;line-height: inherit;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word 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style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><img class=\"\"     width=\"auto\"  style=\"background-color: rgb(238, 237, 235);border-width: 1px;border-style: solid;border-color: rgb(238, 237, 235);background-size: 22px;background-position: center center;background-repeat: no-repeat;height: 374px !important;box-sizing: border-box !important;word-wrap: break-word !important;visibility: visible !important;width: 445px !important;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/08\/beepress8-1533690620.png\" alt=\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u7ec6\u80de\u529f\u80fd\u5b66\u8bc4\u4ef7\u7bc7\" title=\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u7ec6\u80de\u529f\u80fd\u5b66\u8bc4\u4ef7\u7bc7\"  \/><\/p>\n<h2 style=\"font-weight: bold;max-width: 100%;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><\/h2>\n<section class=\"\"   style=\"max-width: 100%;box-sizing: border-box;color: rgb(51, 51, 51);\">\n<section class=\"\"  placeholder=\"\u6df1\u8272\u8fb9\u6846\u6807\u9898\" style=\"margin-top: 10px;margin-bottom: 10px;padding: 10px;max-width: 100%;box-sizing: border-box;line-height: 25px;color: rgb(110, 156, 40);border-radius: 4px;box-shadow: rgb(153, 153, 153) 2px 2px 4px;border-left: 10px solid rgb(184, 222, 127);border-top-color: rgb(184, 222, 127);border-right-color: rgb(184, 222, 127);border-bottom-color: rgb(184, 222, 127);background-color: rgb(229, 243, 208);word-wrap: break-word !important;\">\u7ec6\u80de\u529f\u80fd\u5b66\u8bc4\u4ef7\u65b9\u6cd5\u7684\u5206\u7c7b<\/section>\n<\/section>\n<h2 style=\"font-weight: bold;max-width: 100%;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><\/h2>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">&nbsp;&nbsp;\u6839\u636e\u7ec6\u80de\u5355\u4e00\u7684\u751f\u7406\u53cd\u5e94\u8fdb\u7a0b\u53ef\u4ee5\u5c06\u7ec6\u80de\u7684\u529f\u80fd\u5b66\u68c0\u6d4b\u65b9\u6cd5\u5206\u4e3a\u4ee5\u4e0b4\u7c7b\uff1a<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">1.\u6d4b\u5b9a\u5019\u9009\u836f\u7269\u5f15\u8d77\u9776\u70b9\u86cb\u767d\u6700\u521d\u7684\u53d8\u5316\uff0c\u6700\u5e38\u89c1\u7684\u662f\u5019\u9009\u836f\u7269\u548c\u4f4d\u4e8e\u7ec6\u80de\u819c\u4e0a\u7684\u9776\u70b9\u53d7\u4f53\u7684\u7ed3\u5408\uff0c\u6216\u8005\u963b\u65ad\u9776\u70b9\u53d7\u4f53\u548c\u5929\u7136\u914d\u4f53\u7684\u7ed3\u5408\uff0c\u5e38\u7528\u7684\u68c0\u6d4b\u6280\u672f\u5305\u62ecCell-Basd ELISA\uff0c\u6d41\u5f0f\u7ec6\u80de\u672f\u8fdb\u884c\u6d4b\u5b9a\u5019\u9009\u836f\u7269-\u53d7\u4f53\u5076\u8054\u7269\u3002\u6709\u522b\u4e8e\u5c06\u9776\u70b9\u53d7\u4f53\u91cd\u7ec4\u7eaf\u5316\u51fa\u6765\u8fdb\u884c\u4eb2\u548c\u529b\u8bc4\u4ef7\uff0c\u7ec6\u80de\u819c\u4e0a\u7684\u9776\u70b9\u53d7\u4f53\u66f4\u63a5\u8fd1\u771f\u5b9e\u7684\u751f\u7406\u60c5\u51b5\uff0c\u4f4d\u4e8e\u7ec6\u80de\u4e0a\u7684\u5176\u4ed6\u53d7\u4f53\uff0c\u6548\u5e94\u86cb\u767d\u53ef\u80fd\u4f1a\u53c2\u4e0e\u9776\u70b9\u86cb\u767d\u7684\u6fc0\u6d3b\u53d8\u5316\u8fc7\u7a0b\uff0c\u66f4\u6709\u53c2\u8003\u4ef7\u503c\u3002\u800c\u4e14\u5f88\u591a\u819c\u86cb\u767d\u662f\u591a\u900f\u819c\u7ed3\u6784\uff0c\u5f88\u96be\u7eaf\u5316\u5f97\u5230\u5b8c\u6574\u4e09\u7ea7\u7ed3\u6784\u6784\u8c61\u7684\u53d7\u4f53\u86cb\u767d\uff08\u5982G\u86cb\u767d\u5076\u8054\u53d7\u4f53\u4e3a7\u900f\u819c\uff09\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">2.\u6d4b\u5b9a\u4fe1\u53f7\u8f6c\u5bfc\u8fc7\u7a0b\u4e2d\u4fe1\u53f7\u901a\u8def\u4e2d\u5173\u952e\u751f\u7269\u6807\u5fd7\u7269\u7684\u6d53\u5ea6\uff0c\u5982GPCR\uff08G-\u86cb\u767d\u5076\u8054\u53d7\u4f53\uff09\u6fc0\u6d3b\u8fc7\u7a0b\u4e2d\u7b2c\u4e8c\u4fe1\u4f7fcAMP, DAG, IP3\uff08TR-FRET\uff0cELISA\uff09\u548c Ca<sup style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">+2<\/sup>&nbsp;\uff08FLIPR\uff09\u7684\u6d4b\u5b9a\uff1b\u5173\u952e\u6548\u5e94\u86cb\u767d\u78f7\u9178\u5316\u7b49\u4fee\u9970\u7684\u6d53\u5ea6\uff08TR-FRET\uff0cWB\uff09\uff1b\u5173\u952e\u9176\u9176\u6d53\u5ea6\u7684\u6d4b\u5b9a\uff1b\u52a8\u529b\u5b66\u8f6c\u8fd0\u8fc7\u7a0b\u7684\u6d4b\u5b9a\uff08\u5185\u5316\uff09\u7b49\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">3.\u5bf9\u5f15\u8d77\u7ec6\u80de\u5e94\u7b54\u7684\u8f6c\u5f55\u548c\u7ffb\u8bd1\u6c34\u5e73\u7684\u6d4b\u5b9a\u3002\u5982\u91c7\u7528\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u6280\u672f\u5bf9\u8f6c\u5f55RNA\u6c34\u5e73\u7684\u6d4b\u5b9a\uff1b\u91c7\u7528\u5728\u542f\u52a8\u5b50\u540e\u9762\u63d2\u5165\u5916\u6e90\u7684\u62a5\u544a\u57fa\u56e0\u6d4b\u5b9a\u8f6c\u5f55\u56e0\u5b50\u7684\u6d3b\u6027\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">4.\u4ee5\u6574\u4e2a\u7ec6\u80de\u4e3a\u5bf9\u8c61\u6d4b\u5b9a\u7ec6\u80de\u7684\u589e\u6b96\u6216\u8005\u6b7b\u4ea1\uff08MTT.CCK-8,c,Alarmablue,ATP\u6d4b\u5b9a\uff0cLDH\u6d4b\u5b9a\uff09\uff0c\u7ec6\u80de\u7684\u86cb\u767d\u7ec4\u6216\u8005\u57fa\u56e0\u7ec4\u53d8\u5316\uff08\u6d41\u5f0f\u6d4b\u5b9a\u5468\u671f\u51cb\u4ea1\uff09\uff0c\u7ec6\u80de\u7684\u8f6c\u79fb\u8fc1\u79fb\u80fd\u529b\u53d8\u5316\uff08\u5212\u75d5\uff0c\u4fb5\u88ad\uff0ctranswell\u5b9e\u9a8c\uff09\uff0c\u7ec6\u80de\u5206\u6ccc\u7269\u7684\u6d4b\u5b9a\u5982PBMC\u7ec6\u80de\u7684TNF\u548cIL\u5b9a\u91cf\u6d4b\u5b9a\uff08ELISA,TR-FRET\uff09\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u4ee5\u4e0a\u662f\u7b80\u800c\u6982\u4e4b\u7684\u7ec6\u80de\u529f\u80fd\u5b66\u8bc4\u4ef7\u65b9\u6cd5\uff0c\u4ece\u4e0a\u8ff0\u63cf\u8ff0\u6211\u4eec\u53ef\u4ee5\u770b\u5230\u7ec6\u80de\u529f\u80fd\u5b66\u68c0\u6d4b\u65b9\u6cd5\u591a\u79cd\u591a\u6837\uff0c\u5982\u4f55\u9009\u62e9\u5408\u9002\u7684\u68c0\u6d4b\u65b9\u6cd5\u6765\u6ee1\u8db3\u7814\u7a76\u76ee\u7684\uff1f\u7b14\u8005\u8ba4\u4e3a\u5728\u57fa\u7840\u7814\u7a76\u548c\u9776\u70b9\u786e\u8ba4\u836f\u7269\u7814\u53d1\u521d\u671f\u5e94\u8be5\u7ed3\u5408\u6587\u732e\u8d44\u6599\uff0c\u5c06\u8fdb\u884c\u7ec6\u80de\u5355\u4e00\u7684\u751f\u7406\u53cd\u5e94\u8fdb\u7a0b\u7684\u5404\u4e2a\u9636\u6bb5\u7684\u56db\u7c7b\u529f\u80fd\u5b66\u5b9e\u9a8c\uff0c\u5373\u63a5\u6536\u4fe1\u53f7\uff08\u9776\u70b9\u86cb\u767d\u7ed3\u5408\u529b\uff09\uff0c\u4fe1\u53f7\u653e\u5927\uff08\u7b2c\u4e8c\u4fe1\u4f7f\u6216\u8005\u6fc0\u9176\u6d4b\u5b9a\uff09\uff0c\u8f6c\u5f55\u7ffb\u8bd1\uff08RT-qPCR\u6216\u8005\u62a5\u544a\u57fa\u56e0\uff09\uff0c\u6700\u7ec8\u54cd\u5e94\uff08\u7ec6\u80de\u6b7b\u6d3b\u68c0\u6d4b\uff0c\u5206\u6ccc\u7269\u68c0\u6d4b\uff09\uff0c\u642d\u5efa\u5b8c\u6574\u7684\u836f\u7269\u523a\u6fc0\u540e\u7ec6\u80de\u54cd\u5e94\u7684\u7f51\u7edc\uff0c\u8bc4\u4f30\u9776\u70b9\u7684\u6709\u6548\u6027\u3002\u5728\u836f\u7269\u7b5b\u9009\u9636\u6bb5\uff0c\u5219\u9700\u8981\u6839\u636e\u5b9e\u9a8c\u64cd\u4f5c\u4fbf\u6377\u6027\uff0c\u9009\u62e9\u86cb\u767d\u6c34\u5e73\u7684\u7ed3\u5408\u5b9e\u9a8c\uff081\u7c7b\uff09\u6216\u8005\u7ec6\u80de\u7684\u6b7b\u6d3b\uff084\u7c7b\uff09\u6765\u8fdb\u884c\u836f\u7269\u7684\u521d\u6b65\u9ad8\u901a\u91cf\u7b5b\u9009\u3002\u529f\u80fd\u5b66\u8bc4\u4ef7\u9636\u6bb5\u6700\u91cd\u8981\u7684\u662f\u5bf9\u6d3b\u6027\u6bd4\u8f83\u7eaf\u7684\u5019\u9009\u836f\u7269\u7684\u7ed3\u5408\u529b\u6c34\u5e73\uff081\u7c7b\uff09\u548c\u6700\u7ec8\u54cd\u5e94\u6c34\u5e73\uff084\u7c7b\uff09\u7684\u8bc4\u4f30\u548c\u7cbe\u786e\u7684\u6570\u636e\u5c55\u73b0\uff0c\u4ee5\u5f85\u5efa\u7acb\u548c\u52a8\u7269\u5b9e\u9a8c\u7ed3\u679c\u7684\u5173\u8054\u3002\u8d28\u91cf\u63a7\u5236\u9636\u6bb5\u5219\u662f\u51fa\u4e8e\u65b9\u6cd5\u7684\u7a33\u5b9a\u6027\u548c\u7cbe\u5bc6\u5ea6\u7684\u9700\u6c42\uff0c\u9009\u62e9\u7eaf\u5316\u86cb\u767d\u6c34\u5e73\u7684\u7ed3\u5408\u529b\u8bc4\u4f30\uff084\u7c7b\uff09\u548c\u7ec6\u80de\u529f\u80fd\u5b66\u8bc4\u4f30\u65b9\u6cd5\uff082\uff0c3\uff0c4\u7c7b\uff09\u4ee5\u5907\u653e\u884c\u9700\u8981\u3002<\/span><\/p>\n<h2 style=\"font-weight: bold;max-width: 100%;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><\/h2>\n<section class=\"\"   style=\"max-width: 100%;box-sizing: border-box;color: rgb(51, 51, 51);\">\n<section class=\"\"  placeholder=\"\u6df1\u8272\u8fb9\u6846\u6807\u9898\" style=\"margin-top: 10px;margin-bottom: 10px;padding: 10px;max-width: 100%;box-sizing: border-box;line-height: 25px;color: rgb(110, 156, 40);border-radius: 4px;box-shadow: rgb(153, 153, 153) 2px 2px 4px;border-left: 10px solid rgb(184, 222, 127);border-top-color: rgb(184, 222, 127);border-right-color: rgb(184, 222, 127);border-bottom-color: rgb(184, 222, 127);background-color: rgb(229, 243, 208);word-wrap: break-word !important;\">G-\u86cb\u767d\u5076\u8054\u53d7\u4f53\u548cADCC\u6548\u5e94\u7684\u529f\u80fd\u5b66\u8bc4\u4ef7\u65b9\u6cd5<\/section>\n<\/section>\n<h2 style=\"font-weight: bold;max-width: 100%;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><\/h2>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">G-\u86cb\u767d\u5076\u8054\u53d7\u4f53\u662f\u836f\u7269\u7814\u53d1\u7684\u4e3b\u8981\u9776\u70b9\u4e4b\u4e00\uff0c\u622a\u6b622016\u5e74FDA\u5ba1\u6279\u901a\u8fc7\u76841286\u4e2a\u836f\u7269\u4e2d\u6709460\u4e2a\u662f\u4ee5G-\u86cb\u767d\u5076\u8054\u53d7\u4f53\u4e3a\u9776\u70b9\uff0c\u53601\/3\u4ee5\u4e0a\uff0c\u4e3b\u6d41\u7684\u4eea\u5668\u8bd5\u5242\u4f9b\u5e94\u5546\u63d0\u4f9b\u4e86\u4ee5\u6b64\u9776\u70b9\u7684\u7ec6\u80de\u751f\u7406\u53cd\u5e94\u8fdb\u7a0b\u5404\u4e2a\u9636\u6bb5\u7684\u529f\u80fd\u5b66\u8bc4\u4ef7\u65b9\u6cd5\u3002\u5982\u4e0b\u56fe\u6240\u793a\uff0c\u5728G-\u86cb\u767d\u5076\u8054\u53d7\u4f53\u6fc0\u6d3b\u7684\u7ec6\u80de\u751f\u7406\u53cd\u5e94\u8fdb\u7a0b\u80fd\u591f\u7528\u4e8e\u529f\u80fd\u5b66\u8bc4\u4ef7\u7684\u5404\u4e2a\u5173\u952e\u751f\u7269\u6807\u5fd7\u7269\u3002G-\u86cb\u767d\u5076\u8054\u53d7\u4f53\u88ab\u5019\u9009\u836f\u7269\u6fc0\u6d3b\u540e\uff0cG-\u86cb\u767d\u5076\u8054\u53d7\u4f53\u7684\u7ec6\u80de\u5185\u90e8\u5206\u6784\u578b\u53d1\u751f\u6539\u53d8\u4f7f\u5f97\u5176\u548cG\u86cb\u767d\u7684\u7ed3\u5408\u529b\u589e\u5f3a\uff0cG\u86cb\u767d\u548cG-\u86cb\u767d\u5076\u8054\u53d7\u4f53\u7ed3\u5408\u540e\uff0c\u5176G\u03b1\u4e9a\u57fa\u6d3b\u6027\u53d1\u751f\u6539\u53d8\uff0c\u4f7f\u5f97G\u03b1\u4e9a\u57fa\u66f4\u52a0\u5bb9\u6613\u548cGTP\u7ed3\u5408\uff0c\u5e76\u4ece\u5176\u4e24\u4e2a\u4e9a\u57fa\u4e2d\u89e3\u79bb\u51fa\u6765\uff0cG\u03b1\u4e9a\u57fa\u548cGTP\u7ed3\u5408\u540e\u6839\u636e\u5176\u7c7b\u578b\u7684\u4e0d\u540c\u6539\u53d8\u4e0d\u540c\u6548\u5e94\u86cb\u767d\u7684\u6d3b\u6027\u6fc0\u6d3b\u4e0b\u6e38\u4fe1\u53f7\u7684\u7ea7\u8054\u53cd\u5e94\uff0c\u4f7f\u5f97\u7b2c\u4e8c\u4fe1\u4f7f\u7684\u6d53\u5ea6\u53d1\u751f\u6539\u53d8\uff0c\u7b2c\u4e8c\u4fe1\u4f7f\u7684\u6d53\u5ea6\u53d1\u751f\u6539\u53d8\u6fc0\u6d3b\u540e\u7eed\u7684\u8f6c\u5f55\u56e0\u5b50\u901a\u8def\uff0c\u8f6c\u5f55\u56e0\u5b50\u7684\u6fc0\u6d3b\u8c03\u8282\u7ec6\u80de\u7684\u86cb\u767d\u8868\u8fbe\uff0c\u53d1\u751f\u7ec6\u80de\u5b66\u54cd\u5e94\u3002\u5176\u4e2dG-\u86cb\u767d\u5076\u8054\u53d7\u4f53\u6fc0\u6d3b\u540e\u4f1a\u78f7\u9178\u5316\uff0c\u03b2-arrestin\u548c\u78f7\u9178\u5316\u7684G-\u86cb\u767d\u5076\u8054\u53d7\u4f53\u7ed3\u5408\u540e\u4ecb\u5bfc\u5176\u5185\u5316\uff0c\u5185\u5316\u7684G-\u86cb\u767d\u5076\u8054\u53d7\u4f53\u91cd\u65b0\u56de\u5230\u7ec6\u80de\u8868\u9762\u6216\u8005\u8fdb\u5165\u6eb6\u9176\u4f53\u4e2d\u964d\u89e3\uff0c\u662fG-\u86cb\u767d\u5076\u8054\u53d7\u4f53\u6fc0\u6d3b\u540e\u7684\u62ee\u6297\u8c03\u8282\u901a\u8def\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><img class=\"\"     width=\"521px\"  style=\"background-color: rgb(238, 237, 235);border-width: 1px;border-style: solid;border-color: rgb(238, 237, 235);background-size: 22px;background-position: center center;background-repeat: no-repeat;height: 512.028px !important;box-sizing: border-box !important;word-wrap: break-word !important;width: 521px !important;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/08\/beepress1-1533690621.jpg\" alt=\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u7ec6\u80de\u529f\u80fd\u5b66\u8bc4\u4ef7\u7bc7\" title=\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u7ec6\u80de\u529f\u80fd\u5b66\u8bc4\u4ef7\u7bc7\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u5982\u4e0a\u56fe\u6240\u793a\u5982\u679c\u6309\u7167\u4e0a\u6587\u6240\u8ff0\u7684\u529f\u80fd\u5b66\u8bc4\u4ef7\u7684\u5206\u7c7b\u5206\u4e3a\u56db\u7c7b\uff0c\u4ee5\u2460\uff0c\u2461\uff0c\u2462\uff0c\u2463\u8fdb\u884c\u533a\u5206\uff0c\u540c\u4e00\u7c7b\u522b\u5b9e\u9a8c\u4e0d\u540c\u751f\u7269\u6807\u5fd7\u7269\u7684\u68c0\u6d4b\u65b9\u6cd5\u2461-1\uff0c\u2461-2\u7b49\u8868\u793a\uff0c\u4ee5\u6982\u8ff0\u4e0b\u4e3b\u6d41\u7684\u68c0\u6d4b\u65b9\u6cd5\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\u2460&nbsp;<\/strong><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\u914d\u4f53\u548c\u53d7\u4f53\u7684\u7ed3\u5408\uff0c\u7ec6\u80de\u6c34\u5e73\u7684\u4eb2\u548c\u529b\u8bc4\u4f30\u5b9e\u9a8c\u3002<\/strong><\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">&nbsp; \u5173\u4e8e\u4eb2\u548c\u529b\u8bc4\u4ef7\u7684\u76f8\u5173\u8bba\u8ff0\u7b14\u8005\u5199\u8fc7<strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><em style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u6280\u672f\u4e4b\u4eb2\u548c\u529b\u8bc4\u4ef7\u7bc7<\/em><\/strong>\u53ef\u4f9b\u53c2\u8003\uff0c\u5728\u5b9e\u9645\u7684\u5e94\u7528\u8fc7\u7a0b\u4e2d\uff0c\u7279\u522b\u662f\u65b9\u6cd5\u5f00\u53d1\u7684\u521d\u671f\uff0c\u4ee5\u7ec6\u80de\u8868\u9762\u7684\u86cb\u767d\u4e3a\u9776\u70b9\u7684\u8bc4\u4ef7\u65b9\u6cd5\u591a\u91c7\u7528Cell-Basd ELISA\u6216\u8005\u6d41\u5f0f\u7ec6\u80de\u6280\u672f\u8fdb\u884c\u4eb2\u548c\u529b\u6d4b\u5b9a\uff0c\u6d4b\u5b9a\u7684\u6a21\u5f0f\u53ef\u4ee5\u591a\u6837\uff0c\u5982<strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\u76f4\u63a5\u6cd5\u68c0\u6d4b<\/strong>\uff1a\u5c06\u5019\u9009\u836f\u7269\u8fdb\u884c\u76f4\u63a5\u8fdb\u884c\u8367\u5149\u6807\u8bb0\u68c0\u6d4b\uff0c<strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\u95f4\u63a5\u6cd5\u68c0\u6d4b<\/strong>\uff1a\u6297\u5019\u9009\u836f\u7269\u975e\u9776\u70b9\u7ed3\u5408\u4f4d\u70b9\u7684\u8367\u5149\/\u9176\u8054\u6807\u8bb0\u4e8c\u6297\u8fdb\u884c\u68c0\u6d4b\uff08\u5982\u6297\u4ebaIgG1\u7684Fc\u7aef\u5154\u6297\u4f53\u5076\u8054HRP\u4f5c\u4e3a\u4e8c\u6297\uff09\uff0c\u6216\u8005<strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\u7ade\u4e89\u6cd5\u68c0\u6d4b\uff1a<\/strong>\u5c06\u9776\u70b9\u86cb\u767d\u7684\u5929\u7136\u914d\u4f53\u8fdb\u884c\u6807\u8bb0\uff0c\u52a0\u5165\u5019\u9009\u836f\u7269\u8fdb\u884c\u7ade\u4e89\u3002\u4e00\u822c\u6765\u8bb2<strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\u95f4\u63a5\u6cd5\u68c0\u6d4b<\/strong>\u548c<strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\u7ade\u4e89\u6cd5\u68c0\u6d4b<\/strong>\u66f4\u5177\u6709\u901a\u7528\u6027\u548c\u53ef\u6bd4\u6027\uff0c\u9002\u5408\u591a\u4e2a\u5019\u9009\u836f\u7269\u540c\u65f6\u8fdb\u884c\u7b5b\u9009\u8bc4\u4ef7\u3002\u9700\u8981\u8bf4\u660e\u7684\u662f<strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\u7ade\u4e89\u6cd5\u68c0\u6d4b<\/strong>\u9488\u5bf9\u7684\u662f\u5019\u9009\u836f\u7269\u548c\u9776\u70b9\u86cb\u767d\u4e0a\u5355\u4e00\u7684\u7ed3\u5408\u8868\u4f4d\u4eb2\u548c\u529b\u8bc4\u4f30\uff0c\u800c\u76f4\u63a5\u6cd5\u548c\u95f4\u63a5\u6cd5\u5747\u662f\u5bf9\u5019\u9009\u836f\u7269\u548c\u9776\u70b9\u86cb\u767d\u6574\u4e2a\u7ed3\u6784\u7684\u4eb2\u548c\u529b\u8bc4\u4f30\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\u2461&nbsp;<\/strong><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">Ca<sup style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">2+<\/sup><\/strong><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\uff0c<\/strong><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">IP1<\/strong><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\uff0c<\/strong><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">cAMP<\/strong><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\u7b49\u7b2c\u4e8c\u4fe1\u4f7f\u7684\u68c0\u6d4b<\/strong><\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">&nbsp; &nbsp; &nbsp; Ca<sup style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">2+<\/sup>\u548c\u8367\u5149\u67d3\u6599\uff08Fluo-3AMA\u7b49\uff09\u7ed3\u5408\u540e\u88ab\u7279\u5b9a\u6ce2\u957f\u6fc0\u53d1\u540e\u6709\u53d1\u5c04\u5149\u4ea7\u751f\uff0c\u8be5\u53d1\u5c04\u5149\u7684\u5f3a\u5ea6\u548c\u9499\u79bb\u5b50\u7684\u6d53\u5ea6\u7ebf\u6027\u76f8\u5173\uff0c\u6574\u4e2a\u662f\u91c7\u7528\u8367\u5149\u65b9\u6cd5\u68c0\u6d4b\u57fa\u7840\uff0c\u7531\u4e8e\u7ec6\u80de\u6536\u5230\u523a\u6fc0\u540e\u5176Ca<sup style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">2+<\/sup>\u6d53\u5ea6\u7684\u53d8\u5316\u662f\u4e00\u4e2a\u77ac\u65f6\u7684\u8fc7\u7a0b\uff0c\u4f1a\u5728\u5f88\u77ed\u7684\u65f6\u95f4\u5185\u589e\u52a0\u518d\u56de\u5230\u57fa\u7ebf\u503c\uff0c\u5bf9\u4e8eCa<sup style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">2+<\/sup>\u7684\u68c0\u6d4b\u662f\u4e00\u4e2a\u5b9e\u65f6\u52a8\u6001\u7684\u8fc7\u7a0b\uff0c\u5728\u6574\u4e2a\u5b9e\u9a8c\u8fc7\u7a0b\u4e2d\u52a0\u6837\u548c\u68c0\u6d4b\u90fd\u662f\u9700\u8981\u63a7\u5236\u5728\u6781\u77ed\u7684\u65f6\u95f4\u5185\u5b8c\u6210\uff0cFluorescence Imaging Plate Reader<strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">&nbsp;FLIPR<\/strong>\u2122&nbsp;\u6280\u672f\u548c\u4eea\u5668\u662f\u8fdb\u884c\u77ac\u65f6\u79bb\u5b50\u6d53\u5ea6\u53d8\u5316\u68c0\u6d4b\u7684\u4eea\u5668\uff0c\u5176\u81ea\u52a8\u5316\u8fdb\u6837\uff0c\u5fae\u5b54\u677f\u6a21\u5f0f\uff0c\u5b9e\u65f6\u52a8\u6001\u7684\u68c0\u6d4b\u8fc7\u7a0b\u80fd\u591f\u901a\u8fc7\u63a7\u5236\u5019\u9009\u836f\u7269\u548c\u5929\u7136\u914d\u4f53\u7684\u52a0\u6837\u987a\u5e8f\u548c\u6d53\u5ea6\u5728\u4e00\u6b21\u5b9e\u9a8c\u4e2d\u8fdb\u884c\u9ad8\u901a\u91cf\u7684\uff0c\u5019\u9009\u836f\u7269\u7684\u6fc0\u52a8\u5242\u6548\u679c\uff0c\u589e\u6548\u5242\u6548\u679c\u548c\u62ee\u6297\u5242\u6548\u679c\u7684\u68c0\u6d4b\uff0c\u662f\u7814\u7a76\u79bb\u5b50\u901a\u9053\u548cGPCR\u529f\u80fd\u975e\u5e38\u5b9e\u7528\u7684\u6280\u672f\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><img class=\"\"     width=\"433px\"  style=\"background-color: rgb(238, 237, 235);border-width: 1px;border-style: solid;border-color: rgb(238, 237, 235);background-size: 22px;background-position: center center;background-repeat: no-repeat;height: 439.974px !important;box-sizing: border-box !important;word-wrap: break-word !important;width: 433px !important;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/08\/beepress7-1533690621.jpg\" alt=\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u7ec6\u80de\u529f\u80fd\u5b66\u8bc4\u4ef7\u7bc7\" title=\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u7ec6\u80de\u529f\u80fd\u5b66\u8bc4\u4ef7\u7bc7\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">&nbsp; &nbsp; &nbsp; IP1\uff0ccAMP\u7b49\u5c0f\u5206\u5b50\u7b2c\u4e8c\u4fe1\u4f7f\u4e00\u822c\u53ef\u4ee5\u901a\u8fc7ELISA\uff0cTR-FRET\uff0c\u504f\u632f\u8367\u5149\u7b49\u6280\u672f\u91c7\u7528\u7ade\u4e89\u6cd5\u8fdb\u884c\u5b9a\u91cf\u68c0\u6d4b\uff0c\u5e02\u9762\u4e0a\u5df2\u6709\u591a\u4e2a\u4f9b\u5e94\u5546\u63d0\u4f9b\u76f8\u5173\u8bd5\u5242\u76d2\u8fdb\u884c\u68c0\u6d4b\uff0c\u9700\u8981\u8bf4\u660e\u7684\u662f\u91c7\u7528\u7ade\u4e89\u6cd5\u8fdb\u884c\u68c0\u6d4b\u9700\u8981\u52a0\u5165IP1\uff0ccAMP\u7684\u6807\u51c6\u54c1\u5efa\u7acb\u6807\u51c6\u66f2\u7ebf\u786e\u5b9a\u8be5\u8bd5\u5242\u76d2\u7684\u7ebf\u6027\u68c0\u6d4b\u8303\u56f4\uff0c\u4f18\u5316\u7ec6\u80de\u7684\u52a0\u5165\u6570\u76ee\u3002\u5f88\u591a\u77e5\u540d\u7684\u4f9b\u5e94\u5546\u5b98\u7f51\u4e0a\u6709\u6bd4\u8f83\u8be6\u7ec6\u7684\u8bd5\u5242\u76d2\u8bf4\u660e\u4e66\u548c\u65b9\u6cd5\u4f18\u5316\u65b9\u6848\u53ef\u4f9b\u5b66\u4e60\uff0c\u8fd9\u91cc\u5c31\u4e0d\u4f5c\u8be6\u7ec6\u8ba8\u8bba\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><img class=\"\"     width=\"486px\"  style=\"background-color: rgb(238, 237, 235);border-width: 1px;border-style: solid;border-color: rgb(238, 237, 235);background-size: 22px;background-position: center center;background-repeat: no-repeat;height: 286.658px !important;box-sizing: border-box !important;word-wrap: break-word !important;width: 486px !important;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/08\/beepress5-1533690621.jpg\" alt=\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u7ec6\u80de\u529f\u80fd\u5b66\u8bc4\u4ef7\u7bc7\" title=\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u7ec6\u80de\u529f\u80fd\u5b66\u8bc4\u4ef7\u7bc7\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><img class=\"\"     width=\"553px\"  style=\"background-color: rgb(238, 237, 235);border-width: 1px;border-style: solid;border-color: rgb(238, 237, 235);background-size: 22px;background-position: center center;background-repeat: no-repeat;height: 327.654px !important;box-sizing: border-box !important;word-wrap: break-word !important;width: 553px !important;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/08\/beepress1-1533690621-1.jpg\" alt=\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u7ec6\u80de\u529f\u80fd\u5b66\u8bc4\u4ef7\u7bc7\" title=\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u7ec6\u80de\u529f\u80fd\u5b66\u8bc4\u4ef7\u7bc7\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\u2461<\/strong><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">-5&nbsp;<\/strong>\u78f7\u9178\u5316<\/span>ERK<span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u7684\u68c0\u6d4b<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u5bf9\u4e8e\u78f7\u9178\u5316ERK\u7b49\u5173\u952e\u6fc0\u6d3b\u7684\u5927\u5206\u5b50\u86cb\u767d\u53ef\u91c7\u7528AlphaScreen\u6280\u672f\u8fdb\u884c\u68c0\u6d4b\uff0c\u68c0\u6d4b\u7684\u4e24\u4e2a\u5fae\u7403\u5206\u522b\u6293\u4f4fERK\u86cb\u767d\u548c\u78f7\u9178\u5316\u4f4d\u70b9\uff0c\u901a\u8fc7Donor\u5fae\u7403\u6fc0\u53d1\u540e\u63d0\u4f9b\u80fd\u91cf\uff0c\u5171\u4ef7\u8fde\u63a5\u7269\u7406\u8ddd\u79bb\u8f83\u8fd1\u7684Acceptor\u5fae\u7403\u63a5\u6536\u80fd\u91cf\u540e\u88ab\u6fc0\u53d1\u53d1\u5c04\u5149\u3002\u8be5\u65b9\u6cd5\u80fd\u591f\u907f\u514dTR-FRET\u6280\u672f\u8fdb\u884c\u5927\u5206\u5b50\u5b9a\u91cf\u65f6\u80fd\u91cf\u4f20\u9012\u4f1a\u51fa\u73b0\u6bd4\u8f83\u5927\u7684\u8870\u51cf\u7684\u60c5\u51b5\uff0c\u5f97\u5230\u6bd4\u8f83\u597d\u7684\u4fe1\u566a\u6bd4\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><img class=\"\"     width=\"482px\"  style=\"background-color: rgb(238, 237, 235);border-width: 1px;border-style: solid;border-color: rgb(238, 237, 235);background-size: 22px;background-position: center center;background-repeat: no-repeat;height: 257.747px !important;box-sizing: border-box !important;word-wrap: break-word !important;width: 482px !important;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/08\/beepress5-1533690621-1.jpg\" alt=\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u7ec6\u80de\u529f\u80fd\u5b66\u8bc4\u4ef7\u7bc7\" title=\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u7ec6\u80de\u529f\u80fd\u5b66\u8bc4\u4ef7\u7bc7\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\u2461<\/strong><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">-6&nbsp;<\/strong>G-\u86cb\u767d\u5076\u8054\u53d7\u4f53\u7684\u5185\u5316\u7814\u7a76<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u5982\u4e0b\u56fe\u6240\u793a\u5e38\u89c4\u7684\u5185\u5316\u65b9\u6cd5\u662f\u5c06\u53d7\u4f53\u7684\u914d\u4f53\u8fdb\u884c\u8367\u5149\u6807\u8bb0\uff0c\u548c\u7ec6\u80de\u7ed3\u5408\u5185\u5316\u540e\uff0c\u901a\u8fc7\u9178\u6d17\u6216\u8005\u9176\u5207\u53bb\u9664\u7ec6\u80de\u8868\u9762\u7ed3\u5408\u7684\u8367\u5149\u6807\u8bb0\u914d\u4f53\uff0c\u68c0\u6d4b\u8367\u5149\u6807\u8bb0\u7684\u5f3a\u5ea6\u4ee3\u8868\u5185\u5316\u7684\u5f3a\u5f31\u6216\u8005\u5c06\u914d\u4f53\u8fdb\u884c\u6bd2\u6027\u5206\u5b50\u6807\u8bb0\uff0c\u53d7\u4f53\u548c\u914d\u4f53\u5185\u5316\u8fdb\u5165\u7ec6\u80de\u540e\u91ca\u653e\u6bd2\u6027\u5206\u5b50\uff0c\u901a\u8fc7\u6d4b\u5b9a\u7ec6\u80de\u7684\u6d3b\u6027\u5224\u65ad\u5185\u5316\u7684\u5f3a\u5f31\u3002\u8fd9\u4e24\u79cd\u65b9\u6cd5\u7684\u53ef\u64cd\u4f5c\u6027\u6216\u8005\u7a33\u5b9a\u6027\u76f8\u5bf9\u8f83\u5dee\u3002\u76ee\u524d\u6709pH\u654f\u611f\u578b\u8367\u5149\u67d3\u6599\u53ef\u907f\u514d\u9178\u6d17\u7684\u8fc7\u7a0b\uff0c\u4f7f\u5f97\u672a\u8fdb\u5165\u7ec6\u80de\u7684\u8367\u5149\u67d3\u6599\u7ec6\u80de\u5916\u4e2d\u6027\u6216\u8005\u5f31\u78b1\u6027\u7684\u57f9\u517b\u57fa\u4e2d\u65e0\u8367\u5149\u4fe1\u53f7\uff0c\u5185\u5316\u8fdb\u5165\u7ec6\u80de\u540e\u7684\u9178\u6027\u73af\u5883\u4e2d\u6709\u4fe1\u53f7\u4ea7\u751f\uff0c\u53ef\u4ee5\u907f\u514d\u9178\u6d17\u7684\u5b9e\u73b0\u5b9e\u65f6\u52a8\u6001\u7684\u5185\u5316\u65b9\u6cd5\u68c0\u6d4b\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><img class=\"\"     width=\"534px\"  style=\"background-color: rgb(238, 237, 235);border-width: 1px;border-style: solid;border-color: rgb(238, 237, 235);background-size: 22px;background-position: center center;background-repeat: no-repeat;height: 623.73px !important;box-sizing: border-box !important;word-wrap: break-word !important;width: 534px !important;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/08\/beepress3-1533690621.jpg\" alt=\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u7ec6\u80de\u529f\u80fd\u5b66\u8bc4\u4ef7\u7bc7\" title=\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u7ec6\u80de\u529f\u80fd\u5b66\u8bc4\u4ef7\u7bc7\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\u2462<\/strong><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">&nbsp;<\/strong><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\u62a5\u544a\u57fa\u56e0\u6cd5\u8fdb\u884cADCC<\/strong><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\u6548\u5e94\u68c0\u6d4b\u548c<\/strong><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\u2463<\/strong><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">&nbsp;PBMC<\/strong><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\u6cd5\u8fdb\u884cADCC<\/strong><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\u6548\u5e94\u68c0\u6d4b<\/strong><\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">PBMC\u6cd5\u8fdb\u884cADCC\u6548\u5e94\u68c0\u6d4b\u662f\u91c7\u7528\u65b0\u9c9c\u5916\u5468\u8840\u5355\u6838\u7ec6\u80de(PBMC)\u4f5c\u4e3a\u6548\u5e94\u7ec6\u80de\uff0c\u68c0\u6d4b\u548c\u9776\u7ec6\u80de\u4f5c\u7528\u7684\u6297\u4f53\uff0c\u4ecb\u5bfc\u7684\u6548\u5e94\u7ec6\u80de\u5bf9\u9776\u7ec6\u80de\u7684\u6740\u4f24\u4f5c\u7528\uff0c\u5e38\u7528LDH\u4f5c\u4e3a\u9776\u7ec6\u80de\u6b7b\u4ea1\u7684\u751f\u7269\u6807\u5fd7\u7269\u8fdb\u884c\u5b9a\u91cf\u68c0\u6d4b\uff1b\u800c\u62a5\u544a\u57fa\u56e0\u6cd5\u5982\u4e0b\u56fe\u6240\u793a\u5176\u5b9e\u8d28\u662f\u548c\u9776\u7ec6\u80de\u4f5c\u7528\u7684\u6297\u4f53\uff0c\u542f\u52a8\u4e86\u6548\u5e94\u7ec6\u80de\u7684\u4fe1\u53f7\u901a\u8def\uff0c\u6fc0\u6d3b\u4e86\u8f6c\u5f55\u56e0\u5b50\u8868\u8fbe\u62a5\u544a\u57fa\u56e0\u86cb\u767d\u9176\uff0c\u901a\u8fc7\u5b9a\u91cf\u62a5\u544a\u57fa\u56e0\u86cb\u767d\u9176\uff0c\u8868\u5f81\u6297\u4f53\u7684ADCC\u6d3b\u6027\u3002\u4ece\u65b9\u6cd5\u7684\u771f\u5b9e\u6765\u8bf4PBMC\u6cd5\u66f4\u6709\u8bf4\u670d\u529b\uff0c\u5728\u6297\u4f53ADCC\u529f\u80fd\u7684\u6700\u7ec8\u786e\u8ba4\u9636\u6bb5\u7b14\u8005\u8ba4\u4e3a\u5fc5\u987b\u6709\u8fd9\u6837\u4e00\u6b21\u5b9e\u9a8c\u6570\u636e\u3002\u4f46\u662fPBMC\u6cd5\u4e5f\u6709\u5176\u7f3a\u9677\uff0c\u65b0\u9c9c\u5916\u5468\u8840\u6765\u6e90\u4f7f\u5f97\u8be5\u65b9\u6cd5\u7684\u6750\u6599\u51c6\u5907\u53d8\u5f97\u6210\u672c\u9ad8\u6602\u548c\u590d\u6742\uff0c\u65b0\u9c9c\u8840\u6db2\u7684\u590d\u6742\u6027 \u548c\u6279\u6b21\u5dee\u5f02\u6027\u4e5f\u4f7f\u5f97\u8be5\u65b9\u6cd5\u7684\u7a33\u5b9a\u6027\u548c\u7cbe\u5bc6\u5ea6\u6b20\u7f3a\uff0c\u5982\u4e0b\u56fe\u6240\u793a\uff0cPBMC\u6cd5\u548c\u62a5\u544a\u57fa\u56e0\u6cd5\u5728\u53bbN\u7cd6\u6297\u4f53\u7684ADCC\u6548\u5e94\u68c0\u6d4b\u7ed3\u679c\uff0c\u5728\u8bc1\u5b9e\u4e86\u62a5\u544a\u57fa\u56e0\u6cd5\u548cPBMC\u6cd5\u7684\u7b49\u6548\u6027\u540e\uff0c\u62a5\u544a\u57fa\u56e0\u6cd5\u4f1a\u662f\u66f4\u4f18\u9009\u62e9\uff0c\u5176\u6709\u66f4\u597d\u7684\u7a33\u5b9a\u6027\uff0c\u9002\u5408\u8fdb\u884c\u529f\u80fd\u8bc4\u4ef7\u548c\u8d28\u91cf\u653e\u884c\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><img class=\"\"     width=\"426px\"  style=\"background-color: rgb(238, 237, 235);border-width: 1px;border-style: solid;border-color: rgb(238, 237, 235);background-size: 22px;background-position: center center;background-repeat: no-repeat;height: 280.548px !important;box-sizing: border-box !important;word-wrap: break-word !important;width: 426px !important;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/08\/beepress8-1533690621.jpg\" alt=\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u7ec6\u80de\u529f\u80fd\u5b66\u8bc4\u4ef7\u7bc7\" title=\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u7ec6\u80de\u529f\u80fd\u5b66\u8bc4\u4ef7\u7bc7\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><img class=\"\"     width=\"492px\"  style=\"background-color: rgb(238, 237, 235);border-width: 1px;border-style: solid;border-color: rgb(238, 237, 235);background-size: 22px;background-position: center center;background-repeat: no-repeat;height: 290.657px !important;box-sizing: border-box !important;word-wrap: break-word !important;width: 492px !important;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/08\/beepress6-1533690622.jpg\" alt=\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u7ec6\u80de\u529f\u80fd\u5b66\u8bc4\u4ef7\u7bc7\" title=\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u7ec6\u80de\u529f\u80fd\u5b66\u8bc4\u4ef7\u7bc7\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<h2 style=\"font-weight: bold;max-width: 100%;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><\/h2>\n<section class=\"\"   style=\"max-width: 100%;box-sizing: border-box;color: rgb(51, 51, 51);\">\n<section class=\"\"  placeholder=\"\u6df1\u8272\u8fb9\u6846\u6807\u9898\" style=\"margin-top: 10px;margin-bottom: 10px;padding: 10px;max-width: 100%;box-sizing: border-box;line-height: 25px;color: rgb(110, 156, 40);border-radius: 4px;box-shadow: rgb(153, 153, 153) 2px 2px 4px;border-left: 10px solid rgb(184, 222, 127);border-top-color: rgb(184, 222, 127);border-right-color: rgb(184, 222, 127);border-bottom-color: rgb(184, 222, 127);background-color: rgb(229, 243, 208);word-wrap: break-word !important;\">\u5404\u7c7b\u68c0\u6d4b\u65b9\u6cd5\u7684\u5173\u8054<\/section>\n<\/section>\n<h2 style=\"font-weight: bold;max-width: 100%;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><\/h2>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u73b0\u6709\u7684\u7814\u7a76\u8d44\u6599\u8868\u660e\uff0c\u5404\u7c7b\u68c0\u6d4b\u65b9\u6cd5\u4e4b\u95f4\u6709\u4e00\u5b9a\u7684\u5173\u8054\u6027\uff0c\u4f46\u4e0d\u5177\u7edd\u5bf9\u7684\u76f8\u5173\u6027\uff0c\u5982\u4e0b\u56fe\u4e09\u4e2a\u5019\u9009\u836f\u7269\u5728\u7ec6\u80de\u7684\u751f\u7406\u53cd\u5e94\u8fdb\u7a0b\u7684\u5242\u91cf\u66f2\u7ebf\u6a21\u5f0f\u56fe\uff0c\u7531\u6700\u521d\u76843&gt;2&gt;1,\u53d8\u6210\u6700\u7ec8\u76842&gt;1&gt;3\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><img class=\"\"     width=\"503px\"  style=\"background-color: rgb(238, 237, 235);border-width: 1px;border-style: solid;border-color: rgb(238, 237, 235);background-size: 22px;background-position: center center;background-repeat: no-repeat;height: 323.573px !important;box-sizing: border-box !important;word-wrap: break-word !important;width: 503px !important;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/08\/beepress4-1533690622.jpg\" alt=\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u7ec6\u80de\u529f\u80fd\u5b66\u8bc4\u4ef7\u7bc7\" title=\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u7ec6\u80de\u529f\u80fd\u5b66\u8bc4\u4ef7\u7bc7\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u5373\u4f7f\u662f\u540c\u4e00\u4e2a\u5019\u9009\u836f\u7269\u5728\u4e0d\u540c\u7684\u7ec6\u80de\u4e2d\u4e5f\u6709\u53ef\u80fd\u6709\u4e0d\u540c\u7684\u5242\u91cf\u54cd\u5e94\u66f2\u7ebf<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><img class=\"\"     width=\"419px\"  style=\"background-color: rgb(238, 237, 235);border-width: 1px;border-style: solid;border-color: rgb(238, 237, 235);background-size: 22px;background-position: center center;background-repeat: no-repeat;height: 281.527px !important;box-sizing: border-box !important;word-wrap: break-word !important;width: 419px !important;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/08\/beepress7-1533690622.jpg\" alt=\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u7ec6\u80de\u529f\u80fd\u5b66\u8bc4\u4ef7\u7bc7\" title=\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u7ec6\u80de\u529f\u80fd\u5b66\u8bc4\u4ef7\u7bc7\"  \/><\/p>\n<h2 style=\"font-weight: bold;max-width: 100%;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><\/h2>\n<section class=\"\"   style=\"max-width: 100%;box-sizing: border-box;color: rgb(51, 51, 51);\">\n<section class=\"\"  placeholder=\"\u6df1\u8272\u8fb9\u6846\u6807\u9898\" style=\"margin-top: 10px;margin-bottom: 10px;padding: 10px;max-width: 100%;box-sizing: border-box;line-height: 25px;color: rgb(110, 156, 40);border-radius: 4px;box-shadow: rgb(153, 153, 153) 2px 2px 4px;border-left: 10px solid rgb(184, 222, 127);border-top-color: rgb(184, 222, 127);border-right-color: rgb(184, 222, 127);border-bottom-color: rgb(184, 222, 127);background-color: rgb(229, 243, 208);word-wrap: break-word !important;\">\u529f\u80fd\u5b66\u65b9\u6cd5\u5f00\u53d1\u7684\u5b9e\u8d28<\/section>\n<\/section>\n<h2 style=\"font-weight: bold;max-width: 100%;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><\/h2>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;line-height: 1.6;box-sizing: border-box !important;word-wrap: break-word !important;\">\u7ec6\u80de\u7684\u751f\u7406\u53cd\u5e94\u8fdb\u7a0b\u662f\u4e00\u4e2a\u590d\u6742\u8fde\u7eed\u7684\u8fc7\u7a0b,\u7531\u6700\u521d\u7ec6\u80de\u63a5\u6536\u4fe1\u53f7\u5230\u6700\u7ec8\u7ec6\u80de\u505a\u51fa\u54cd\u5e94,\u4fe1\u53f7\u5728\u7ec6\u80de\u5185\u7684\u4fe1\u53f7\u4f20\u5bfc\u548c\u653e\u5927\u662f\u8fde\u7eed\u7684,\u591a\u79cd\u56e0\u7d20\u4f5c\u7528\u7684,\u975e\u7ebf\u6027\u7684\u8fc7\u7a0b,\u800c\u5404\u4e2a\u68c0\u6d4b\u65b9\u6cd5\u63d0\u4f9b\u7684\u5b9e\u9a8c\u7ed3\u679c\u4fe1\u606f\u5747\u662f\u7247\u6bb5\u5316\u7684\u4fe1\u606f,\u529f\u80fd\u5b66\u8bc4\u4ef7\u65b9\u6cd5\u80fd\u591f\u505a\u5230\u8fd9\u4e9b\u7247\u6bb5\u5316\u4fe1\u606f\u7684\u5c55\u73b0\u548c\u6536\u96c6,\u65e0\u6cd5\u5bf9\u5019\u9009\u836f\u7269\u7684\u4f18\u52a3\u9884\u671f\u505a\u51fa\u80af\u5b9a\u7684\u5224\u65ad\uff0c\u4f46\u53ef\u4ee5\u4e3a\u540e\u7eed\u7684\u52a8\u7269\u5b66\u5b9e\u9a8c\u4e43\u81f3\u4e34\u5e8a\u5b9e\u9a8c\u63d0\u4f9b\u4fe1\u606f\u8fdb\u884c\u4e00\u5b9a\u7a0b\u5ea6\u7684\u5173\u8054\uff0c\u603b\u7ed3\u7ecf\u9a8c\u3002\u800c\u5728\u6574\u4e2a\u4fe1\u606f\u7684\u6536\u96c6\u8fc7\u7a0b\uff0c<\/span><strong style=\"max-width: 100%;font-size: 14px;line-height: 1.6;box-sizing: border-box !important;word-wrap: break-word !important;\">\u6570\u636e\u7684\u771f\u5b9e\u6027\u662f\u975e\u5e38\u91cd\u8981\u7684\uff0c\u800c\u529f\u80fd\u5b66\u8bc4\u4ef7\u65b9\u6cd5\u7684\u51c6\u786e\u6027\u548c\u771f\u5b9e\u6027\u9700\u8981\u4ece\u5b9e\u9a8c\u65b9\u6cd5\u672c\u8eab\u53bb\u89e3\u8bfb\u3002<\/strong><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u56de\u987e\u4ee5\u4e0a\u7684\u529f\u80fd\u5b66\u5b9e\u9a8c\uff0c\u6240\u6709\u7684\u529f\u80fd\u5b66\u8bc4\u4ef7\u7684\u5b9e\u8d28\u53ea\u6709\u4e24\u4e2a\uff0c\u4eb2\u548c\u529b\u6d4b\u5b9a\u548c\u751f\u7269\u6807\u5fd7\u7269\u7684\u6d53\u5ea6\u6d4b\u5b9a\u3002\u4eb2\u548c\u529b\u6d4b\u5b9a\u662f\u57fa\u7840\uff0c\u6709\u7ed3\u5408\u624d\u53ef\u80fd\u6709\u529f\u80fd\u3002\u7531\u7ed3\u5408\u5f15\u8d77\u7684\u7ec6\u80de\u7684\u4fe1\u53f7\u4f1a\u7ecf\u8fc7\u591a\u91cd\u7684\u4f20\u9012\u548c\u653e\u5927\uff0c\u65e0\u8bba\u91c7\u7528\u4f55\u79cd\u68c0\u6d4b\u6280\u672f\uff0c\u5bf9\u8fd9\u4e2a\u8fc7\u7a0b\u4e2d\u5404\u4e2a\u751f\u7269\u6807\u5fd7\u7269\u7684\u7cbe\u786e\u5b9a\u91cf\u662f\u529f\u80fd\u8bc4\u4ef7\u7684\u5173\u952e\uff0cCa<sup style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">2+<\/sup>\uff0cIP1\uff0ccAMP\u7684\u5b9a\u91cf\uff0c\u78f7\u9178\u5316ERK\u7684\u5b9a\u91cf\uff0c\u8424\u706b\u866b\u7d20\u9176\u7684\u5b9a\u91cf\uff0c\u6d3b\u7ec6\u80de\/\u6b7b\u7ec6\u80de\u4e2a\u6570\u7684\u5b9a\u91cf\uff0c\u800c\u5728\u5b9a\u91cf\u8fc7\u7a0b\u4e2d\u4fe1\u53f7\u548c\u751f\u7269\u6807\u5fd7\u7269\u6d53\u5ea6\u5e94\u8be5\u662f\u7ebf\u6027\u76f8\u5173\u7684\uff0c\u8fd9\u4e2a\u9690\u853d\u7684\u524d\u63d0\u6761\u4ef6\u5f88\u591a\u65f6\u5019\u662f\u88ab\u5ffd\u7565\u7684\uff0c\u4e5f\u662f\u529f\u80fd\u5b66\u8bc4\u4ef7\u65b9\u6cd5\u7684\u5b9e\u9a8c\u6570\u636e\u88ab\u9519\u8bef\u7684\u89e3\u8bfb\uff0c\u6ca1\u6709\u5f88\u597d\u7684\u7a33\u5b9a\u6027\u7684\u5173\u952e\u539f\u56e0\u3002\u5019\u9009\u836f\u7269\u7ed3\u5408\u5f15\u8d77\u7684\u7ec6\u80de\u5185\u7684\u4fe1\u53f7\u7684\u975e\u7ebf\u6027\u4f20\u9012\u662f\u7ec6\u80de\u751f\u7406\u53cd\u5e94\u8fdb\u7a0b\u7684\u5ba2\u89c2\u53cd\u5e94\uff0c\u800c\u68c0\u6d4b\u4fe1\u53f7\u548c\u751f\u7269\u6807\u5fd7\u7269\u7684\u975e\u7ebf\u6027\u5173\u7cfb\u662f\u4e00\u4e2a\u9519\u8bef\u3002\u8fd9\u6837\u7684\u6982\u5ff5\u7684\u5f62\u6210\u548c\u7406\u89e3\u63a8\u8350\u8bfb\u8005\u770b\u770b\u7b14\u8005\u6700\u521d\u7684\u4e00\u7bc7\u6587\u6863<em style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">ELISA<\/em><em style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\u6280\u672f\u5e94\u7528\u6982\u89c8\u548c\u65b9\u6cd5\u5b66\u5f00\u53d1\u521d\u6790\u3002<\/em><\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">&nbsp; &nbsp; &nbsp; &nbsp;\u5b66\u597dELISA\uff0c\u73a9\u8f6c\u751f\u7269\u529f\u80fd\u5b66\u8bc4\u4ef7\u3002\u4e00\u5207\u4f3c\u4e4e\u53c8\u56de\u5230\u4e86\u539f\u70b9\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u53c2\u8003\u8d44\u6599\uff1a<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 10px;box-sizing: border-box !important;word-wrap: break-word !important;\">1.&nbsp;&nbsp;Kenakin T P. A Pharmacology Primer: Theory, Applications, and Methods[J]. Academic Pr Inc, 2009.<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 10px;box-sizing: border-box !important;word-wrap: break-word !important;\">2.&nbsp;&nbsp;Swarnakar S, Dhar T K. Assay Development: Fundamentals and Practices. By Ge Wu.[J]. Chembiochem, 2015, 12(4):648-649.<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 10px;box-sizing: border-box !important;word-wrap: break-word !important;\">3.&nbsp;&nbsp;Sittampalam G S, Gal-Edd N, Arkin M, et al. FLIPR\u2122 Assays for GPCR and Ion Channel Targets &#8212; Assay Guidance Manual[J]. 1990.<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 10px;box-sizing: border-box !important;word-wrap: break-word !important;\">4.&nbsp;https:\/\/www.promega.com<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 10px;box-sizing: border-box !important;word-wrap: break-word !important;\">5.&nbsp; http:\/\/www.perkinelmer.com.cn\/category\/lance-tr-fret<\/span><\/p>\n<p style=\"font-size: 16px;white-space: normal;max-width: 100%;min-height: 1em;text-align: center;box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;font-size: 20px;font-family: -apple-system-font, BlinkMacSystemFont, Arial, sans-serif;letter-spacing: 0.544px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;color: rgb(255, 0, 0);box-sizing: border-box !important;word-wrap: break-word !important;\">\u8bc6\u522b\u5fae\u4fe1\u4e8c\u7ef4\u7801\uff0c\u6dfb\u52a0\u751f\u7269\u5236\u54c1\u5708\u5c0f\u7f16\uff0c\u7b26\u5408\u6761\u4ef6\u8005\u5373\u53ef\u52a0\u5165\u751f\u7269\u5236\u54c1\u5708\u5fae\u4fe1\u7fa4\uff01<\/span><\/strong><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"font-size: 16px;white-space: normal;max-width: 100%;min-height: 1em;letter-spacing: 0.544px;font-family: -apple-system-font, BlinkMacSystemFont, Arial, sans-serif;text-align: center;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 18px;box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;color: rgb(0, 209, 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border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 14px;color: rgb(105, 115, 117);font-family: \u5fae\u8f6f\u96c5\u9ed1;letter-spacing: 1px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u672c\u516c\u4f17\u53f7\u6240\u6709\u8f6c\u8f7d\u6587\u7ae0\u7cfb\u51fa\u4e8e\u4f20\u9012\u66f4\u591a\u4fe1\u606f\u4e4b\u76ee\u7684\uff0c\u4e14\u660e\u786e\u6ce8\u660e\u6765\u6e90\u548c\u4f5c\u8005\uff0c\u4e0d\u5e0c\u671b\u88ab\u8f6c\u8f7d\u7684\u5a92\u4f53\u6216\u4e2a\u4eba\u53ef\u4e0e\u6211\u4eec\u8054\u7cfb(cbplib@163.com)\uff0c\u6211<span style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span>\u4eec\u5c06\u7acb\u5373\u8fdb\u884c\u5220\u9664\u5904\u7406\u3002\u6240\u6709\u6587\u7ae0\u4ec5\u4ee3\u8868\u4f5c\u8005\u89c2\u70b9\uff0c\u4e0d\u4ee3\u8868\u672c\u7ad9\u7acb\u573a\u3002<\/span><\/section>\n<p style=\"white-space: normal;max-width: 100%;min-height: 1em;font-size: 14px;line-height: 25.6px;color: rgb(105, 115, 117);letter-spacing: 1px;font-family: \u5fae\u8f6f\u96c5\u9ed1;box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;line-height: 28px;color: rgb(255, 0, 0);box-sizing: border-box !important;word-wrap: break-word !important;\"><img class=\"\"     style=\"background-color: rgb(238, 237, 235);border-width: 1px;border-style: solid;border-color: rgb(238, 237, 235);background-size: 22px;background-position: center center;background-repeat: no-repeat;line-height: 28.4444px;box-sizing: border-box !important;word-wrap: break-word !important;visibility: visible !important;width: 640px !important;\" width=\"auto\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/08\/beepress2-1533690622.jpeg\" alt=\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u7ec6\u80de\u529f\u80fd\u5b66\u8bc4\u4ef7\u7bc7\" title=\"\u751f\u7269\u529f\u80fd\u5b66\u7b5b\u9009\u8bc4\u4ef7\u4e4b\u7ec6\u80de\u529f\u80fd\u5b66\u8bc4\u4ef7\u7bc7\"  \/><\/strong><\/p>\n<blockquote class='keep-source'>\n<p>\u59cb\u53d1\u4e8e\u5fae\u4fe1\u516c\u4f17\u53f7\uff1a                         \u751f\u7269\u5236\u54c1\u5708                      <\/p>\n<\/blockquote>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u6458\u8981\uff1a&nbsp;&nbsp; \u73b0\u6709\u7684\u836f\u7269\u7814\u53d1\u7684\u6587\u732e\u548c\u8d44\u6599\u5c06\u7ec6\u80de\u76f8\u5173\u7684\u529f\u80fd\u5b66\u8bc4\u4ef7\u5206\u5272\u6210\u4e00\u4e2a\u4e2a\u72ec\u7acb\u7684\u5b9e\u9a8c\u6280\u672f\u3002\u672c [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":19386,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[2],"tags":[],"_links":{"self":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/19385"}],"collection":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/comments?post=19385"}],"version-history":[{"count":1,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/19385\/revisions"}],"predecessor-version":[{"id":19405,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/19385\/revisions\/19405"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media\/19386"}],"wp:attachment":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media?parent=19385"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/categories?post=19385"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/tags?post=19385"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}