{"id":19456,"date":"2018-07-11T01:00:00","date_gmt":"2018-08-08T01:10:42","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/2018\/08\/08\/%e6%b7%b1%e5%9c%b3%e5%85%88%e8%bf%9b%e9%99%a2%e5%9c%a8-crispr-%e5%9f%ba%e5%9b%a0%e7%bc%96%e8%be%91%e5%ba%94%e7%94%a8%e9%a2%86%e5%9f%9f%e5%8f%96%e5%be%97%e6%96%b0%e7%aa%81%e7%a0%b4\/"},"modified":"2018-08-08T09:10:44","modified_gmt":"2018-08-08T01:10:44","slug":"%e6%b7%b1%e5%9c%b3%e5%85%88%e8%bf%9b%e9%99%a2%e5%9c%a8-crispr-%e5%9f%ba%e5%9b%a0%e7%bc%96%e8%be%91%e5%ba%94%e7%94%a8%e9%a2%86%e5%9f%9f%e5%8f%96%e5%be%97%e6%96%b0%e7%aa%81%e7%a0%b4","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/19456\/","title":{"rendered":"\u6df1\u5733\u5148\u8fdb\u9662\u5728 CRISPR \u57fa\u56e0\u7f16\u8f91\u5e94\u7528\u9886\u57df\u53d6\u5f97\u65b0\u7a81\u7834"},"content":{"rendered":"
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\u5bfc\u8bfb\uff1a<\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/strong><\/span><\/strong><\/strong><\/span>\u8fd1\u65e5\uff0c\u4e2d\u56fd\u79d1\u5b66\u9662\u6df1\u5733\u5148\u8fdb\u6280\u672f\u7814\u7a76\u9662\u7814\u7a76\u5458\u55bb\u5b66\u950b\u8bfe\u9898\u7ec4\u5728 CRISPR \u57fa\u56e0\u7f16\u8f91\u5e94\u7528\u9886\u57df\u53d6\u5f97\u65b0\u7a81\u7834\u3002\u76f8\u5173\u5de5\u4f5c Enhanced Cytosolic Delivery and Releases of CRISPR\/Cas9 by Black Phosphorus Nanosheets for Genome Editing\uff08\u300a\u7528\u4e8e\u57fa\u56e0\u7f16\u8f91\u7684\u9ed1\u78f7\u589e\u5f3a\u578b CRISPR\/Cas9 \u8fd0\u8f7d\u4e0e\u91ca\u653e\u7cfb\u7edf\u300b\uff09\u53d1\u8868\u4e8e\u300a\u5fb7\u56fd\u5e94\u7528\u5316\u5b66\u300b\uff08Angewandte Chemie International Edition\uff0cDOI: 10.1002\/anie.201806941\uff0c10.1002\/ange.201806941\uff09\u3002\u8bba\u6587\u5171\u540c\u7b2c\u4e00\u4f5c\u8005\u662f\u535a\u58eb\u5468\u6587\u534e\u548c\u7814\u7a76\u52a9\u7406\u5d14\u6d69\u4e1c\uff0c\u901a\u8baf\u4f5c\u8005\u662f\u55bb\u5b66\u950b\uff0c\u53c2\u4e0e\u4f5c\u8005\u8fd8\u5305\u62ec\u5e1d\u56fd\u7406\u5de5\u5b66\u9662\u6559\u6388 Ying Liming<\/span>\u3002<\/span><\/p>\n


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\"\u6df1\u5733\u5148\u8fdb\u9662\u5728<\/p>\n

\u56fe 1: \u57fa\u4e8e\u9ed1\u78f7\u7eb3\u7c73\u7247\u7684 Cas9-sgRNA \u590d\u5408\u4f53\u8f6c\u8fd0\u4f53\u7cfb\u7ec6\u80de\u5185\u4f5c\u7528\u793a\u610f\u56fe\u3002<\/span><\/p>\n


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\u8fd1\u65e5\uff0c\u4e2d\u56fd\u79d1\u5b66\u9662\u6df1\u5733\u5148\u8fdb\u6280\u672f\u7814\u7a76\u9662\u7814\u7a76\u5458\u55bb\u5b66\u950b\u8bfe\u9898\u7ec4\u5728 CRISPR \u57fa\u56e0\u7f16\u8f91\u5e94\u7528\u9886\u57df\u53d6\u5f97\u65b0\u7a81\u7834\u3002\u76f8\u5173\u5de5\u4f5c Enhanced Cytosolic Delivery and Releases of CRISPR\/Cas9 by Black Phosphorus Nanosheets for Genome Editing\uff08\u300a\u7528\u4e8e\u57fa\u56e0\u7f16\u8f91\u7684\u9ed1\u78f7\u589e\u5f3a\u578b CRISPR\/Cas9 \u8fd0\u8f7d\u4e0e\u91ca\u653e\u7cfb\u7edf\u300b\uff09\u53d1\u8868\u4e8e\u300a\u5fb7\u56fd\u5e94\u7528\u5316\u5b66\u300b\uff08Angewandte Chemie International Edition\uff0cDOI: 10.1002\/anie.201806941\uff0c10.1002\/ange.201806941\uff09\u3002\u8bba\u6587\u5171\u540c\u7b2c\u4e00\u4f5c\u8005\u662f\u535a\u58eb\u5468\u6587\u534e\u548c\u7814\u7a76\u52a9\u7406\u5d14\u6d69\u4e1c\uff0c\u901a\u8baf\u4f5c\u8005\u662f\u55bb\u5b66\u950b\uff0c\u53c2\u4e0e\u4f5c\u8005\u8fd8\u5305\u62ec\u5e1d\u56fd\u7406\u5de5\u5b66\u9662\u6559\u6388 Ying Liming\u3002<\/span><\/p>\n

\"\u6df1\u5733\u5148\u8fdb\u9662\u5728<\/p>\n

\u56fe 2: \u57fa\u4e8e\u9ed1\u78f7\u7eb3\u7c73\u7247\u7684 Cas9-sgRNA \u590d\u5408\u4f53\u8f6c\u8fd0\u4f53\u7cfb\u7684\uff08a\uff09\u80de\u8d28\u8f93\u8fd0\u4ee5\u53ca\u6838\u8f6c\u8fd0\u548c\uff08b\uff09\u9ed1\u78f7\u7eb3\u7c73\u7247\u5728\u7ec6\u80de\u5185\u7684\u964d\u89e3\u8fc7\u7a0b\u3002<\/span><\/p>\n


<\/p>\n

\u8fd1\u5e74\u6765\uff0cCRISPR\/Cas9 \u7cfb\u7edf\u4f5c\u4e3a\u4e00\u79cd\u6e90\u81ea\u539f\u6838\u751f\u7269\u83b7\u5f97\u6027\u514d\u75ab\u7cfb\u7edf\u7684\u57fa\u56e0\u5b9a\u70b9\u7f16\u8f91\u6280\u672f\uff0c\u5df2\u88ab\u5e7f\u6cdb\u5e94\u7528\u4e8e\u8bf8\u5982\u57fa\u56e0\u8868\u8fbe\u8c03\u63a7\u3001\u57fa\u56e0\u7f16\u8f91\u4ee5\u53ca\u6838\u9178\u7684\u539f\u4f4d\u68c0\u6d4b\u7b49\u9886\u57df\u3002\u7136\u800c\uff0c\u7531\u4e8e\u4f20\u7edf\u7684\u9488\u5bf9\u7f16\u7801 DNA \u7684\u8f6c\u8fd0\u624b\u6bb5\u5b58\u5728\u7740\u6613\u5bfc\u81f4\u9776\u7ec6\u80de\u7684\u63d2\u5165\u7f3a\u5931\u548c\u8fc7\u8868\u8fbe\uff0c\u5e76\u5f15\u8d77\u57fa\u56e0\u53d8\u5f02\u548c\u8131\u9776\u6548\u5e94\u7b49\u95ee\u9898\uff0c\u9650\u5236\u4e86\u8be5\u6280\u672f\u7684\u4e34\u5e8a\u5e94\u7528\u6f5c\u529b\u3002\u56e0\u6b64\uff0c\u5f00\u53d1\u51fa\u4e00\u79cd\u65b0\u7684\u9488\u5bf9 Cas9-sgRNA \u6838\u9178 – \u86cb\u767d\u590d\u5408\u4f53\u7684\u9ad8\u6548\u7ec6\u80de\u8f6c\u8fd0 \/ \u91ca\u653e\u624b\u6bb5\u5c06\u5177\u6709\u91cd\u8981\u7684\u79d1\u5b66\u610f\u4e49\u548c\u5e94\u7528\u524d\u666f\u3002<\/span><\/p>\n


<\/p>\n

\u9ed1\u78f7\u56e0\u5176\u826f\u597d\u7684\u751f\u7269\u6d3b\u6027\u548c\u751f\u7269\u76f8\u5bb9\u6027\uff0c\u4ee5\u53ca\u5176\u4f18\u8d8a\u7684\u5149\u7535\u7279\u6027\uff0c\u5728\u751f\u7269\u533b\u5b66\u9886\u57df\u53d7\u5230\u5e7f\u6cdb\u5173\u6ce8\u3002\u55bb\u5b66\u950b\u8bfe\u9898\u7ec4\u5728\u4e4b\u524d\u7814\u7a76\u4e2d\u5df2\u6210\u529f\u5c06\u9ed1\u78f7\u7eb3\u7c73\u6750\u6599\u5e94\u7528\u4e8e\u5149\u58f0\u6210\u50cf\uff08Small, 2017, 13, 1602896\uff09\u3001\u80bf\u7624\u5149\u70ed\u6cbb\u7597\uff08Nature Communications, 2016, 7, 12967\uff09\u3001\u80bf\u7624\u653e\u5c04\u6cbb\u7597\uff08Biomaterials, 2018, 171, 12\uff09\u3001\u5149\u81f4\u5f62\u53d8\u690d\u5165\u6750\u6599\uff08Biomaterials, 2018, 164, 11\uff09\u4ee5\u53ca\u6c34\u51dd\u80f6\u6577\u6599\uff08Advanced Science, 2018, 5, 1700848\uff09\u7b49\u9886\u57df\u3002\u57fa\u4e8e\u8fd9\u4e9b\u524d\u671f\u5de5\u4f5c\uff0c\u4ee5\u53ca\u5728\u8fd9\u4e9b\u5de5\u4f5c\u4e2d\u53d1\u73b0\u7684\u9ed1\u78f7\u964d\u89e3\u6240\u5f15\u8d77\u7684\u7ec6\u80de\u5185\u4f53\uff08endosome\uff09\u7834\u88c2\u73b0\u8c61\uff0c\u56e2\u961f\u63d0\u51fa\u9ed1\u78f7\u7eb3\u7c73\u7247\u53ef\u7528\u4e8e\u751f\u7269\u6d3b\u6027\u5927\u5206\u5b50\u7684\u7ec6\u80de\u8f6c\u8fd0\u548c\u91ca\u653e\u3002<\/span><\/p>\n

\"\u6df1\u5733\u5148\u8fdb\u9662\u5728<\/p>\n

\u56fe 3: \u57fa\u4e8e\u9ed1\u78f7\u7eb3\u7c73\u7247\u7684 Cas9-sgRNA \u590d\u5408\u4f53\u8f6c\u8fd0\u4f53\u7cfb\uff1a\uff08a\uff09\u5728\u7ec6\u80de\u682a MCF- 7 \u5185\u5b9e\u73b0\u9ad8\u6548\u7684\u57fa\u56e0\u7f16\u8f91\uff1b\uff08b\uff09\u5728\u7ec6\u80de\u682a EGFP\/A549 \u53ca\uff08c\uff09\u52a8\u7269\u8377\u7624\u6a21\u578b\u7684 EGFP \u4f4d\u70b9\u5b9e\u73b0\u4e86\u9ad8\u6548\u7684\u57fa\u56e0\u6c89\u9ed8\u548c\u57fa\u56e0\u7f16\u8f91\u3002<\/span><\/p>\n


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\u5728\u8be5\u7814\u7a76\u4e2d\uff0c\u8bfe\u9898\u7ec4\u4ee5\u8d85\u8584\u4e8c\u7ef4\u9ed1\u78f7\u7eb3\u7c73\u7247\u4e3a\u8f7d\u4f53\uff0c\u901a\u8fc7\u9759\u7535\u76f8\u4e92\u4f5c\u7528\u5bf9\u643a\u5e26\u4e09\u6838\u5b9a\u4f4d\u5e8f\u5217\uff083xNLS\uff09\u7684 Cas9-sgRNA \u590d\u5408\u4f53\u5b9e\u73b0\u9ad8\u6548\u8d1f\u8f7d\uff0c\u4ece\u800c\u6784\u5efa\u4e86\u4e00\u79cd\u9ad8\u6548\u7684 CRISPR\/Cas9 \u57fa\u56e0\u8fd0\u8f7d\u4f53\u7cfb\uff08\u56fe 1\uff09\u3002\u8be5\u8f6c\u8fd0\u4f53\u7cfb\u4e3b\u8981\u901a\u8fc7\u76f4\u63a5\u7834\u819c\u548c\u80de\u541e\u4f5c\u7528\u8fdb\u5165\u7ec6\u80de\u5185\u4f53\uff0c\u8fdb\u5165\u5185\u4f53\u7684\u9ed1\u78f7\u8f7d\u4f53\u5728\u9178\u6027\u73af\u5883\u4e0b\u8fc5\u901f\u964d\u89e3\u4e3a\u9ad8\u5ea6\u751f\u7269\u76f8\u5bb9\u7684\u65e0\u673a\u78f7\u9178\u76d0\uff0c\u4ece\u800c\u5bfc\u81f4\u7531\u6e17\u900f\u538b\u6539\u53d8\u6240\u5f15\u8d77\u7684\u5185\u4f53\u7834\u88c2\uff0c\u5b9e\u73b0 Cas9-sgRNA \u590d\u5408\u4f53\u7684\u9ad8\u6548\u80de\u5185\u91ca\u653e\uff0c\u5e76\u6700\u7ec8\u7531\u6240\u643a\u5e26\u7684\u4e09\u6838\u5b9a\u4f4d\u5e8f\u5217\u9776\u5411\u8fdb\u5165\u7ec6\u80de\u6838\uff0c\u5b8c\u6210\u57fa\u56e0\u7f16\u8f91\u7684\u529f\u80fd\uff08\u56fe 2\uff09\u3002\u5b9e\u9a8c\u7ed3\u679c\u663e\u793a\uff0c\u76f8\u5bf9\u4e8e\u5176\u4ed6\u7eb3\u7c73\u6750\u6599\u8f7d\u4f53\uff0c\u8be5\u57fa\u4e8e\u9ed1\u78f7\u7eb3\u7c73\u7247\u7684 Cas9-sgRNA \u590d\u5408\u4f53\u8f6c\u8fd0\u4f53\u7cfb\u53ef\u5728\u8f83\u4f4e\u6d53\u5ea6\u4e0b\u5b8c\u6210\u5bf9\u4e0d\u540c\u7ec6\u80de\u682a\u4ee5\u53ca\u52a8\u7269\u8377\u7624\u6a21\u578b\u7684\u9ad8\u6548\u57fa\u56e0\u7f16\u8f91\u548c\u57fa\u56e0\u6c89\u9ed8\uff08\u56fe 3\uff09\u3002\u8fd9\u4e00\u57fa\u4e8e\u9ed1\u78f7\u7eb3\u7c73\u7247\u7684\u9ad8\u6548\u7ec6\u80de\u8f6c\u8fd0\u4f53\u7cfb\u53ef\u8fdb\u4e00\u6b65\u7528\u4e8e\u5176\u4ed6\u751f\u7269\u6d3b\u6027\u5927\u5206\u5b50\u7684\u9ad8\u6548\u7ec6\u80de\u8f6c\u8fd0\uff0c\u4ece\u800c\u5177\u6709\u91cd\u8981\u7684\u533b\u5b66\u7814\u7a76\u548c\u4e34\u5e8a\u5e94\u7528\u4ef7\u503c\u3002\u76f8\u5173\u5de5\u4f5c\u5df2\u7533\u8bf7\u4e13\u5229\uff08CN108148874A\uff09\uff0c\u5e76\u4f9d\u6258\u5b75\u5316\u7684\u4e2d\u79d1\u58a8\u78f7\u79d1\u6280\u6709\u9650\u516c\u53f8\uff08MoPhos.cn\uff09\uff0c\u79ef\u6781\u63a8\u8fdb\u76f8\u5173\u6280\u672f\u7684\u4ea7\u4e1a\u5316\u3002<\/span><\/p>\n


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\u53c2\u8003\u6587\u732e\uff1a<\/span><\/strong><\/span><\/p>\n

Wenhua Zhou, Haodong Cui, Liming Ying,  Xue-Feng Yu  et al. Enhanced Cytosolic Delivery and Releases of CRISPR\/Cas9 by Black Phosphorus Nanosheets for Genome Editing<\/strong>.  25 June 2018 https:\/\/doi.org\/10.1002\/anie.201806941<\/span><\/p>\n

\u8bc6\u522b\u5fae\u4fe1\u4e8c\u7ef4\u7801\uff0c\u6dfb\u52a0\u751f\u7269\u5236\u54c1\u5708\u5c0f\u7f16\uff0c\u7b26\u5408\u6761\u4ef6\u8005\u5373\u53ef\u52a0\u5165\u751f\u7269\u5236\u54c1\u5708\u5fae\u4fe1\u7fa4\uff01<\/span><\/strong>
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\u8bf7\u6ce8\u660e\uff1a\u59d3\u540d+\u7814\u7a76\u65b9\u5411\uff01<\/span><\/strong><\/span><\/p>\n

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\"\u6df1\u5733\u5148\u8fdb\u9662\u5728<\/p>\n<\/section>\n


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\u672c\u516c\u4f17\u53f7\u6240\u6709\u8f6c\u8f7d\u6587\u7ae0\u7cfb\u51fa\u4e8e\u4f20\u9012\u66f4\u591a\u4fe1\u606f\u4e4b\u76ee\u7684\uff0c\u4e14\u660e\u786e\u6ce8\u660e\u6765\u6e90\u548c\u4f5c\u8005\uff0c\u4e0d\u5e0c\u671b\u88ab\u8f6c\u8f7d\u7684\u5a92\u4f53\u6216\u4e2a\u4eba\u53ef\u4e0e\u6211\u4eec\u8054\u7cfb(cbplib@163.com)\uff0c\u6211<\/span>\u4eec\u5c06\u7acb\u5373\u8fdb\u884c\u5220\u9664\u5904\u7406\u3002\u6240\u6709\u6587\u7ae0\u4ec5\u4ee3\u8868\u4f5c\u8005\u89c2\u70b9\uff0c\u4e0d\u4ee3\u8868\u672c\u7ad9\u7acb\u573a\u3002<\/span><\/section>\n

\"\u6df1\u5733\u5148\u8fdb\u9662\u5728<\/strong><\/p>\n<\/section>\n

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\u59cb\u53d1\u4e8e\u5fae\u4fe1\u516c\u4f17\u53f7\uff1a \u751f\u7269\u5236\u54c1\u5708 <\/p>\n<\/blockquote>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

\u5bfc\u8bfb\uff1a\u8fd1\u65e5\uff0c\u4e2d\u56fd\u79d1\u5b66\u9662\u6df1\u5733\u5148\u8fdb\u6280\u672f\u7814\u7a76\u9662\u7814\u7a76\u5458\u55bb\u5b66\u950b\u8bfe\u9898\u7ec4\u5728 CRISPR \u57fa\u56e0\u7f16\u8f91\u5e94\u7528\u9886\u57df\u53d6\u5f97\u65b0\u7a81\u7834\u3002\u76f8\u5173\u5de5 […]<\/p>\n","protected":false},"author":1,"featured_media":19457,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[2],"tags":[],"_links":{"self":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/19456"}],"collection":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/comments?post=19456"}],"version-history":[{"count":1,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/19456\/revisions"}],"predecessor-version":[{"id":19467,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/19456\/revisions\/19467"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media\/19457"}],"wp:attachment":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media?parent=19456"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/categories?post=19456"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/tags?post=19456"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}