{"id":25546,"date":"2018-08-06T01:00:00","date_gmt":"2018-08-09T03:36:27","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/2018\/08\/09\/case-study-%ef%bc%9atgn1412%e5%85%8d%e7%96%ab%e6%b2%bb%e7%96%97%e6%80%a7%e6%8a%97%e4%bd%93%e5%ae%89%e5%85%a8%e6%80%a7%e7%9a%84%e4%bd%93%e5%a4%96%e6%b5%8b%e5%ae%9a\/"},"modified":"2018-08-09T11:36:30","modified_gmt":"2018-08-09T03:36:30","slug":"case-study-%ef%bc%9atgn1412%e5%85%8d%e7%96%ab%e6%b2%bb%e7%96%97%e6%80%a7%e6%8a%97%e4%bd%93%e5%ae%89%e5%85%a8%e6%80%a7%e7%9a%84%e4%bd%93%e5%a4%96%e6%b5%8b%e5%ae%9a","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/25546\/","title":{"rendered":"Case study \uff1aTGN1412\u514d\u75ab\u6cbb\u7597\u6027\u6297\u4f53\u5b89\u5168\u6027\u7684\u4f53\u5916\u6d4b\u5b9a"},"content":{"rendered":"
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\"Case<\/section>\n<\/section>\n
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\u524d\u8a00\uff1a<\/span><\/strong>\u968f\u7740PD-1\u6297\u4f53\u9002\u5e94\u75c7\u7684\u4e0d\u65ad\u6269\u5c55\uff0c\u56fd\u5916O\u836f\u548cK\u836f\u76f8\u7ee7\u5728\u4e2d\u56fd\u7533\u8bf7\u4e0a\u5e02\uff0c\u56fd\u5185\u4fe1\u8fbe\uff0c\u541b\u5b9e\u548c\u6052\u745e\u4e89\u53d6PD-1\u6297\u4f53\u56fd\u4ea7\u4e0a\u5e02\u9996\u53d1,\u6297\u4f53\u4f5c\u4e3a\u4eba\u4f53\u514d\u75ab\u8c03\u8282\u751f\u7269\u5236\u5242\u5df2\u88ab\u4e1a\u754c\u6240\u719f\u77e5\uff0c\u4e5f\u6709\u8d8a\u6765\u8d8a\u591a\u7684\u836f\u4f01\u5f00\u59cb\u5e03\u5c40\u80bf\u7624\u514d\u75ab\u9886\u57df\u3002\u5728\u4e1a\u754c\u90fd\u671f\u76fc\u4e0b\u4e00\u4e2a\u660e\u661f\u514d\u75ab\u6297\u4f53\u7684\u8bde\u751f\u65f6\uff0c\u5c0f\u7f16\u5728\u6b64\u5206\u4eab\u4e00\u4e2aCD28\u7684\u6fc0\u52a8\u5242\u6297\u4f53TGN1412\u4e34\u5e8a\u4e00\u671f\u5931\u8d25\u7684\u6848\u4f8b\uff0c\u5e76\u6df1\u6316National Institute for Biological Standards and Control (NIBSC) \u5173\u4e8e\u6b64\u6297\u4f53\u7684\u56de\u987e\u6027\u6587\u7ae0\uff0c\u5177\u4f53\u5206\u6790\u8be5\u6297\u4f53\u514d\u75ab\u6bd2\u6027\u4f53\u5916\u6d4b\u5b9a\u7684\u5404\u79cd\u65b9\u6cd5\uff0c\u5f3a\u8c03\u8f83\u4e3a\u5b8c\u5584\u7684\u514d\u75ab\u6cbb\u7597\u6027\u6297\u4f53\u5b89\u5168\u6027\u8bc4\u4ef7\u7684\u4f53\u5916\u6d4b\u5b9a\u65b9\u6cd5\u548c\u6570\u636e\u89e3\u8bfb\u5728\u6574\u4e2a\u514d\u75ab\u6cbb\u7597\u6297\u4f53\u7814\u53d1\u8fc7\u7a0b\u7684\u91cd\u8981\u5730\u4f4d\u3002<\/span><\/section>\n


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\"Case<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n
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\u80cc\u666f\u4ecb\u7ecd\uff1a<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

TGN1412\u662f\u4e00\u6b3e\u9776\u5411<\/span>T\u7ec6\u80de\u7684\u5171\u523a\u6fc0\u53d7\u4f53<\/span>CD28<\/span>\u7684\u8d85\u7ea7\u6fc0\u52a8\u5242\uff0c\u5728<\/span>2006<\/span>\u5e74\u8be5\u6297\u4f53\u7b2c\u4e00\u6b21\u8fdb\u5165\u4eba\u4f53\u4e34\u5e8a\u5b9e\u9a8c\u65f6\uff0c<\/span>6<\/span>\u4e2a\u5065\u5eb7\u7684\u53d7\u8bd5\u8005\u5728\u6ce8\u5c04\u8be5\u6297\u4f53\u7684<\/span>90<\/span>\u5206\u949f\u4e4b\u5185\u4fbf\u4ea7\u751f\u4e86\u4e25\u91cd\u7684\u5371\u673a\u751f\u547d\u5b89\u5168\u7684\u7ec6\u80de\u56e0\u5b50\u98ce\u66b4\u3002\u5f00\u53d1\u8be5\u6297\u4f53\u7684\u516c\u53f8<\/span>TeGenero Immuno Therapeutics\u4e5f\u5728\u4e00\u5e74\u540e\u5ba3\u5e03\u7834\u4ea7\u3002\u4e8b\u540e\u7684\u5206\u6790\u786e\u8ba4\u7528\u4e8e\u4e34\u5e8a\u5b9e\u9a8c\u6297\u4f53\u7684\u6210\u54c1\u662f\u7b26\u5408\u6807\u51c6\u89c4\u8303\u7684\uff0c\u5c24\u5176\u662f\u6392\u9664\u4e86\u53ef\u80fd\u5f15\u8d77\u7ec6\u80de\u56e0\u5b50\u98ce\u66b4\u6210\u56e0\u7684\u5185\u6bd2\u7d20\u7684\u6c61\u67d3\u3002\u8fd9\u8bf4\u660e\u4e86\u5728\u4e34\u5e8a\u524d\u7814\u53d1\u9636\u6bb5<\/span>TGN1412\u7684\u514d\u75ab\u6bd2\u6027\u5b9e\u9a8c\u7ed3\u679c\u5bf9\u4eba\u4f53\u5185\u7684\u5b89\u5168\u6027\u9884\u6d4b\u7684\u5931\u8d25\u3002\u8fd9\u4e9b\u4e34\u5e8a\u524d\u7684\u5b9e\u9a8c\u5305\u62ec\u4f53\u5916\u4eba\u548c\u5176\u4ed6\u7269\u79cd\u7684\u6dcb\u5df4\u7ec6\u80de\uff0c\u5168\u8840\u7ec6\u80de\u7684\u7ec6\u80de\u56e0\u5b50\u91ca\u653e\u7684\u6d4b\u5b9a\uff0c\u4eb2\u7f18\u76f8\u8fd1\u7684\u7075\u957f\u7c7b\u54fa\u4e73\u52a8\u7269\u98df\u87f9\u7334\u7684\u4f53\u5185\u6d4b\u5b9a\u3002\u540e\u7eed\u7684\u5206\u6790\u663e\u793a\u5bf9<\/span>TGN1412\u8d77\u54cd\u5e94\u7684\u7684\u662f\u4eba\u7684\u6548\u5e94\u8bb0\u5fc6\u6027<\/span>T\u7ec6\u80de\uff0c\u548c\u4eba\u4f53\u4e0d\u540c\uff0c\u98df\u87f9\u7334\u4e2d\u8be5\u4e9a\u578b\u7684<\/span>T\u7ec6\u80de\u4f4e\u8868\u8fbe\u6216\u8005\u4e0d\u8868\u8fbe<\/span>CD28<\/span>\u53d7\u4f53\uff0c\u8fd9\u5f88\u597d\u7684\u89e3\u91ca\u4e86\u98df\u87f9\u7334\u7684\u5b9e\u9a8c\u7ed3\u679c\u5bf9\u4e34\u5e8a\u8bd5\u9a8c\u7684\u9884\u6d4b\u5931\u8d25\uff0c\u5373\u4f7f\u5728\u98df\u87f9\u7334<\/span>50mg\/Kg,\u800c\u4eba\u4f53\u7684\u6ce8\u5c04\u5242\u91cf<\/span>0.1 mg\/kg\u7684\u60c5\u51b5\u4e0b\u3002\u90a3\u4e48\u5728\u4f53\u5916\u5b9e\u9a8c\u4e2d\u91c7\u7528\u540c\u79cd\u5c5e\u7684\u4eba\u7684\u6dcb\u5df4\u7ec6\u80de\u548c\u4eba\u5168\u8840\u7ec6\u80de\u4e3a\u4ec0\u4e48\u6ca1\u6709\u8d77\u5230\u9884\u6d4b\u4e34\u5e8a\u5b9e\u9a8c\u514d\u75ab\u6bd2\u6027\u7684\u4f5c\u7528\u4e86\uff1f\u8fd9\u662f\u6b64\u7bc7\u6587\u7ae0\u5c0f\u7f16\u8981\u7740\u91cd\u53d9\u8ff0\u7684\uff0c\u5b9e\u9a8c\u65b9\u6cd5\u7684\u7ec6\u8282\u5bf9\u5b9e\u9a8c\u7ed3\u679c\u4ea7\u751f\u7684\u5dee\u5f02\u6027\u5f71\u54cd\u3002<\/span><\/span><\/p>\n

\"Case<\/p>\n

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\u4f53\u5916\u514d\u75ab\u5b89\u5168\u6027\u6d4b\u5b9a\u65b9\u6cd5\u8bbe\u8ba1\uff1a<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

TGN1412\u6297\u4f53\u7684\u4f53\u5916\u65b9\u6cd5\u7684\u4f5c\u7528\u6d53\u5ea6\u4ece<\/span>0.5\u5230<\/span> 1000 \u03bcg\/ml\uff0c\u6b64\u6d53\u5ea6\u8303\u56f4\u4e0e\u4eba<\/span>\/\u98df\u87f9\u7334\u4f53\u5185\u6297\u4f53\u7684\u4f5c\u7528\u6d53\u5ea6\u76f8\u5bf9\u5e94\u3002<\/span><\/span><\/p>\n

TGN1412\u6297\u4f53\u5728\u4eba<\/span>\/\u98df\u87f9\u7334\u7684<\/span>PBMC<\/span>\u4e2d\u7684\u4f5c\u7528\u65b9\u5f0f<\/span>NIBSC\u8bbe\u7f6e\u4e86\u5982\u4e0b\u56fe<\/span>6\u79cd\u4f5c\u7528\u6a21\u5f0f<\/span>Protocol 1(P1)- Protocol 6(P6)<\/span>\u3002<\/span><\/span><\/p>\n

P1\u548c<\/span>P2<\/span>\u4e3a\u6db2\u76f8\u4f5c\u7528\u6a21\u5f0f\uff0c\u5373<\/span>TGN1412\u6297\u4f53\u5728\u6eb6\u6db2\u73af\u5883\u548c<\/span>PBMC\u7ec6\u80de\u8fdb\u884c\u4f5c\u7528\u3002<\/span>P2<\/span>\u76f8\u8f83<\/span>P1<\/span>\uff0c\u52a0\u5165\u4e86\u6297<\/span>TGN1412 Fc\u7aef\u7684\u6297\u4f53\uff0c\u53ef\u4ee5\u4f7f\u5f97<\/span>TGN1412\u5728\u6db2\u76f8\u4e2d\u5f62\u6210\u591a\u4ef7\u7684\u805a\u4f53\u590d\u5408\u7269\u3002\u8fd9\u4e24\u79cd\u65b9\u6cd5\u4e5f\u662f\u5e38\u89c4\u901a\u7528\u7684\uff0c\u4e5f\u662f\u6700\u521d<\/span>\u7528\u4e8e<\/span>TGN1412\u514d\u75ab\u5b89\u5168\u6027\u7684\u6d4b\u5b9a\u65b9\u6cd5\u3002<\/span><\/span><\/p>\n

P3-P6\u4e3a\u56fa\u76f8<\/span>–<\/span>\u6db2\u76f8\u4f5c\u7528\u6a21\u5f0f\u3002<\/span>TGN1412\u5305\u88ab\u5438\u9644\u56fa\u5b9a\u5728\u56fa\u4f53\u754c\u9762\u4e0a\u548c\u5904\u4e8e\u6db2\u76f8\u5185\u7684<\/span>PBMC\u76f8\u4e92<\/span>\u4f5c\u7528\uff0c\u6b64\u7c7b\u65b9\u6cd5\u7c7b\u4f3c\u4e8e<\/span>ELISA<\/span>\u7684\u4f5c\u7528\u6a21\u5f0f\uff0c<\/span>P3-P6<\/span> TGN1412\u56fa\u5b9a\u7684\u6a21\u5f0f\u4e0d\u4e00\u6837\uff0c<\/span>P3\u91c7\u7528\u7684<\/span>TGN1412\u76f4\u63a5\u91c7\u7528\u5305\u88ab\u6db2\u5305\u88ab\u5728<\/span>96\u5b54\u805a\u82ef\u4e59\u70ef\u6750\u8d28\u7684\u7ec4\u7ec7\u7ec6\u80de\u57f9\u517b\u677f\u4e2d\uff08\u6e7f\u6cd5\u5305\u88ab\uff09\uff0c\u800c<\/span>P4<\/span>\u91c7\u7528\u7684<\/span>TGN1412\u5305\u88ab<\/span>96\u5b54\u805a\u82ef\u4e59\u70ef\u677f\u4e2d\u98ce\u5e72\u4ee5\u5f97\u5230\u66f4\u52a0\u7a33\u5b9a\u548c\u9971\u548c\u7684\u56fa\u5b9a\u6548\u679c\uff08\u5e72\u6cd5\u5305\u88ab\uff09\u3002<\/span>P5,P6<\/span>\u8003\u8651\u5230\u76f4\u63a5\u5305\u88ab\u5728\u56fa\u76f8\u4e2d\u53ef\u80fd\u5bfc\u81f4<\/span>TGN1412\u7684\u4f4d\u70b9\u5c4f\u853d\u73b0\u8c61\uff0c\u4e3a\u95f4\u63a5\u5305\u88ab\uff0c<\/span>P5\u4e3a\u5e72\u6cd5\u5305\u88ab\u6297<\/span>Fc<\/span>\u6297\u4f53\u540e\u518d\u6e7f\u6cd5\u5305\u88ab<\/span>TGN1412\uff0c<\/span>P6\u5219\u662f\u6a21\u62df\u4f53\u5185\u73af\u5883\uff0c\u5148\u94fa\u4e0a\u4e00\u5c42\u5185\u76ae\u7ec6\u80de\u540e\u518d\u52a0\u5165<\/span>TGN1412\u6db2\u4f53\u3002<\/span>P3-P6\u8fd9\u56db\u79cd\u6a21\u5f0f\u662f<\/span>TGN1412\u514d\u75ab\u5b89\u5168\u6027\u4e34\u5e8a\u524d\u4f53\u5916\u672a\u8fdb\u884c\u8fc7\u5b9e\u9a8c\u8bbe\u8ba1\u3002<\/span><\/span> <\/span><\/span><\/p>\n

\"Case<\/p>\n

\u540c\u65f6\u6d4b\u5b9a\u65b9\u6cd5<\/span>\u91c7\u7528\u4e86<\/span>PHA\uff0c\u81f4\u6709\u4e1d\u5206\u88c2\u4eba<\/span>CD3<\/span>\u6fc0\u52a8\u6297\u4f53<\/span>UCHT-1(R&D Systems)\uff0c\u4e0d\u81f4\u6709\u4e1d\u5206\u88c2\u6052\u6cb3\u7334<\/span>CD3\u6297\u4f53<\/span>(clone FN-18; Abcam)\uff0c\u6297\u4eba<\/span> CD28 Ab (clone CD28.2; BD Biosciences)\u7b49\u4f5c\u4e3a\u9633\u6027\u5bf9\u7167\u3002<\/span><\/span><\/span>
<\/p>\n

\u6d4b\u5b9a\u4e86\u4ee5\u4e0b\u7684\u6307\u6807\uff1a1.\u91c7\u7528ELISA\u5bf9PBMC\u4e2d\u7ec6\u80de\u56e0\u5b50\u7684\u91ca\u653e\u542b\u91cf\u8fdb\u884c\u68c0\u6d4b 2.\u91c7\u7528PKH26\u7ec6\u80de\u819c\u8367\u5149\u67d3\u6599\u5bf9PBMC\u4e2d\u6dcb\u5df4\u7ec6\u80de\u7684\u6fc0\u6d3b\u548c\u6269\u589e\u8fdb\u884c\u6d41\u5f0f\u6d4b\u5b9a\u30023. \u91c7\u7528\u6d41\u5f0f\u6d4b\u5b9aT\u6dcb\u5df4\u7ec6\u80de\u7684\u6fc0\u6d3b\u548cbrefeldin A (2\u03bcg\/well =36\u03bcM\uff09\u4f5c\u7528\u4e0b\u80de\u5185\u65e9\u671f\u7ec6\u80de\u56e0\u5b50\u4fe1\u53f7\u7684\u6d4b\u5b9a\u3002<\/span><\/p>\n

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\u5b9e\u9a8c\u7ed3\u679c\u5206\u6790<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

PBMC\u548c\u5168\u8840\u4e2d\u7ec6\u80de\u56e0\u5b50\u7684\u91ca\u653e<\/span><\/span><\/strong><\/span><\/p>\n

\u6db2\u76f8\u4f5c\u7528\u6a21\u5f0f<\/span>P1,P2\u5b9e\u9a8c\u65b9\u6848\u5728<\/span>TGN1412\u7684\u9ad8\u4f4e\u5242\u91cf\u6d53\u5ea6\u4e0b\u548c\u4eba<\/span>PBMC\u4f5c\u7528\u5747\u672a\u68c0\u6d4b\u5230\u76f8\u5bf9\u4e8e\u9634\u6027\u5bf9\u7167\u57fa\u7ebf\u6d53\u5ea6\u4ee5\u4e0a\u7684\u7ec6\u80de\u56e0\u5b50<\/span> TNF-\u03b1, IL-2, IL-6, IL-8, and IFN-\u03b3\u7684\u91ca\u653e\uff0c\u6b64\u7ed3\u679c\u548c<\/span>TGN1412\u4e34\u5e8a\u524d\u514d\u75ab\u5b89\u5168\u6027\u7684\u6d4b\u5b9a\u7ed3\u679c\u662f\u4e00\u81f4\u7684\u3002\u4e0e\u6b64\u7ed3\u679c\u76f8\u53cd\u7684\u662f\uff0c<\/span>P4\u5b9e\u9a8c\u65b9\u6848\u5728<\/span>3<\/span>\u6b21\u72ec\u7acb\u91cd\u590d\u5b9e\u9a8c\u4e2d\u5747\uff0c\u5f53<\/span>TGN1412\u5e72\u6cd5\u5305\u88ab\u5728<\/span>96\u5b54\u677f\u7684\u542b\u91cf\u8fbe\u5230<\/span>10<\/span>\u03bcg\/well\u548c<\/span>1<\/span>\u03bcg\/well\u65f6\uff0c\u4e0e\u4eba<\/span>PBMC\u6216\u8005<\/span>20%<\/span>\uff08<\/span>v\/v<\/span>\uff09\u7684\u4eba\u5168\u8840\u4f5c\u7528\u65f6\uff0c\u68c0\u6d4b\u5230\u8fdc\u9ad8\u4e8e\u80cc\u666f\u7684<\/span>TNF-\u03b1, IL-6, and IL-8\u7684\u91ca\u653e\u3002\u800c<\/span>P4\u5b9e\u9a8c\u65b9\u6848\u5728\u98df\u87f9\u7334\u7684<\/span>20%\uff08<\/span>v\/v<\/span>\uff09\u7684\u7334\u5168\u8840\u4e2d\u4e5f\u672a\u68c0\u6d4b\u5230\u9ad8\u4e8e\u80cc\u666f\u7684<\/span>TNF-\u03b1, IL-6, and IL-8\u7684\u91ca\u653e\u3002<\/span><\/span><\/p>\n

\"Case<\/p>\n

\u540c\u65f6\uff0c\u91c7\u7528\u56fa<\/span>–\u6db2<\/span>P6<\/span>\u5b9e\u9a8c\u65b9\u6848\uff08\u94fa\u677f\u5185\u76ae\u7ec6\u80de\u540e\u518d\u52a0\u5165<\/span>TGN1424<\/span>\uff09\uff0c\u4e0e\u4eba<\/span>PBMC<\/span>\u5171\u57f9\u517b\u4e5f\u68c0\u6d4b\u5230\u5927\u91cf\u663e\u8457\u7684\u7ec6\u80de\u56e0\u5b50\u91ca\u653e\u3002<\/span><\/span><\/p>\n

\u6dcb\u5df4\u7ec6\u80de\u7684\u589e\u6b96\u6d4b\u5b9a<\/span><\/strong><\/span><\/p>\n

\u4e0e\u7ec6\u80de\u56e0\u5b50\u7684\u6d4b\u5b9a\u7ed3\u679c\u7c7b\u4f3c\uff0c\u91c7\u7528\u6db2\u76f8\u4f5c\u7528\u6a21\u5f0f\u672a\u68c0\u6d4b\u5230\u4eba\u6dcb\u5df4\u7ec6\u80de\u7684\u6269\u589e\uff0c\u800c\u91c7\u7528\u56fa<\/span>–\u6db2\u4f5c\u7528\u6a21\u5f0f\u7684<\/span>P4<\/span>\u65b9\u6848\u4e2d\uff0c\u5f53\u5e72\u6cd5\u56fa\u5b9a<\/span>1\u03bcg\/well\u7684<\/span>TGN1412\u80fd\u591f\u68c0\u6d4b\u5230\u663e\u8457\u7684\u4eba\u6dcb\u5df4\u7ec6\u80de\u7684\u589e\u6b96\uff0c\u5176\u5728<\/span>3\u5929\u7684\u57f9\u517b\u8fc7\u7a0b\u4e2d\uff0c<\/span>TGN1412\u523a\u6fc0\u7684\u589e\u6b96\u7684\u4eba\u6dcb\u5df4\u7ec6\u80de\u9ad8\u8fbe<\/span>35%\uff0c\u5982\u4e0b\u56fe\u6240\u793a\uff1a<\/span><\/span><\/p>\n

\"Case <\/span><\/p>\n

\u5728<\/span>P5\u56fa<\/span>–<\/span>\u6db2\u7684\u4f5c\u7528\u65b9\u6848\uff08\u5e72\u6cd5\u5305\u88ab\u6297<\/span>Fc<\/span>\u6297\u4f53<\/span>+<\/span>\u6e7f\u6cd5\u5305\u88ab<\/span>TGN1412\uff09\u4e2d\u751a\u81f3\u68c0\u6d4b\u5230\u66f4\u9ad8\u7684\u4eba\u6dcb\u5df4\u7ec6\u80de\u589e\u6b96\u7387\uff0c\u8fd9\u8bf4\u660e\u5373\u4f7f\u662f<\/span>P4\u65b9\u6848\uff08\u5e72\u6cd5\u5305\u88ab<\/span>TGN1412\uff09\u5bf9\u4eba\u6dcb\u5df4\u7ec6\u80de\u589e\u6b96\u7684\u5f71\u54cd\u4e5f\u88ab\u4f4e\u4f30\u4e86\u3002\u4e0e\u6b64\u622a\u7136\u76f8\u53cd\uff0c\u5728\u540c\u6837\u91c7\u7528<\/span>P4\u6a21\u5f0f\uff0c\u53ea\u68c0\u6d4b\u5230\u6781\u5176\u5fae\u5f31\u7684\u7334\u6dcb\u5df4\u7ec6\u80de\u589e\u6b96\u3002<\/span><\/span><\/p>\n

\u6dcb\u5df4\u7ec6\u80de\u7684\u6fc0\u6d3b\u548c\u80de\u5185\u65e9\u671f\u7ec6\u80de\u56e0\u5b50\u91ca\u653e<\/span><\/strong><\/span><\/p>\n

\u91c7\u7528<\/span>P4\u6a21\u5f0f\u5e72\u6cd5\u56fa\u5b9a<\/span>TGN1412\u5206\u522b\u548c\u4eba\u6216\u7334\u7684\u6dcb\u5df4\u7ec6\u80de\u4f5c\u7528\uff0c\u5747\u80fd\u591f\u68c0\u6d4b\u5230<\/span>CD4+<\/span><\/sup>\u6dcb\u5df4\u7ec6\u80de\u7684\u6fc0\u6d3b\u7684\u751f\u7269\u6807\u5fd7\u7269<\/span>CD25(IL-2R\u03b1)\u7684\u4e0a\u8c03\uff0c\u4f46\u662f\u7334\u7684\u6dcb\u5df4\u7ec6\u80de\u5e76\u672a\u68c0\u6d4b\u5230\u540e\u7eed\u7684\u589e\u6b96\u73b0\u8c61<\/span>,\u5982\u4e0b\u56fe\uff1a<\/span><\/span><\/p>\n

\"Case<\/p>\n

\u5f53\u5bf9\u6dcb\u5df4\u7ec6\u80de\u80de\u5185\u65e9\u671f\u5f62\u6210\u7684\u7ec6\u80de\u56e0\u5b50<\/span>IL-2\u8fdb\u884c\u68c0\u6d4b\u65f6\uff0c<\/span>P4<\/span>\u65b9\u6848\u548c<\/span>P5<\/span>\u65b9\u6848\u5747\u68c0\u6d4b\u5230\u663e\u8457\u7684\u80de\u5185\u7ec6\u80de\u56e0\u5b50<\/span>IL-2<\/span>\uff0c\u548c\u6dcb\u5df4\u7ec6\u80de\u7684\u589e\u6b96\u6d4b\u5b9a\u7ed3\u679c\u7c7b\u4f3c\uff0c<\/span>P5<\/span>\u65b9\u6848\u68c0\u6d4b\u5230\u7684\u9633\u6027\u7ec6\u80de\u56e0\u5b50<\/span>IL-2<\/span>\u7684\u7ec6\u80de\u8981\u8fdc\u9ad8\u4e8e<\/span>P4<\/span>\u65b9\u6848\uff0c\u518d\u6b21\u8bf4\u660e\u5373\u4f7f\u540c\u6837\u662f\u9633\u6027\u7ed3\u679c\uff0c\u4e0d\u540c\u7684\u5b9e\u9a8c\u65b9\u6848\u5c06\u4f1a\u5e26\u6765\u4e0d\u540c\u7684\u7ed3\u679c\u3002<\/span><\/span><\/p>\n

\"Case<\/p>\n

\n
\n
\n
\n

\u5b9e\u9a8c\u7ed3\u679c\u8ba8\u8bba\uff1a<\/p>\n<\/section>\n<\/section>\n<\/section>\n


<\/section>\n

CD28\u7279\u5f02\u6027\u7684\u5355\u514b\u9686\u6297\u4f53\u6fc0\u52a8\u5242\u5728\u4e34\u5e8a\u5b9e\u9a8c\u4e2d\u663e\u793a\u4e86\u4e25\u91cd\u5371\u53ca\u751f\u547d\u7684\u7ec6\u80de\u56e0\u5b50\u98ce\u66b4\u7684\u526f\u4f5c\u7528\u3002\u4e3a\u4ec0\u4e48\u5728\u8fdb\u5165\u4eba\u4f53\u5b9e\u9a8c\u524d\uff0c<\/span>TGN1412\u4e34\u5e8a\u524d\u7684\u5b89\u5168\u6027\u6d4b\u8bd5\u548c\u98df\u87f9\u7334\u4f53\u5185\u7684\u6d4b\u8bd5\u5747\u6ca1\u6709\u5bf9\u8be5\u6297\u4f53\u6f5c\u5728\u523a\u6fc0\u7684\u7ec6\u80de\u56e0\u5b50\u91ca\u653e\u548c\u6dcb\u5df4\u7ec6\u80de\u7684\u589e\u6b96\u8fdb\u884c\u6210\u529f\u7684\u9884\u6d4b\uff1f\u8fd9\u4e2a\u95ee\u9898\u6b64\u7bc7\u6587\u7ae0\u4e2d\u4e0d\u540c\u7684\u5b9e\u9a8c\u65b9\u6848\u5bfc\u81f4\u4e0d\u540c\u7684\u5b9e\u9a8c\u7ed3\u679c\u4e2d\u627e\u5230\u7b54\u6848\u3002<\/span>P1, P2\u4f53\u5916\u5b9e\u9a8c\u65b9\u6848\u5e76\u4e0d\u80fd\u5f88\u597d\u6a21\u62df\u4eba\u4f53\u5185<\/span>TGN1412\u548c\u4e0e\u4eba\u514d\u75ab\u7ec6\u80de\u7684\u76f8\u4e92\u4f5c\u7528\uff0c\u8fd9\u4e5f\u662f<\/span>TGN1412\u4e34\u5e8a\u524d\u5b89\u5168\u6027\u4f53\u5916\u6d4b\u5b9a\u7684\u65b9\u6848\u3002\u4f53\u5916\u548c\u4f53\u5185\u7ed3\u679c\u7684\u56de\u987e\u6027\u6bd4\u8f83\u663e\u793a\uff0c\u4f53\u5916\u7684<\/span>P4\uff0c<\/span>P5<\/span>\uff0c<\/span>P6<\/span>\u5b9e\u9a8c\u65b9\u6848\uff08\u56fa<\/span>–<\/span>\u6db2\u4f5c\u7528\u6a21\u5f0f\uff0c\u5408\u9002\u8db3\u591f\u7684<\/span>TGN1412\u5242\u91cf\uff09\u53ef\u80fd\u66f4\u52a0\u8d34\u8fd1\u4f53\u5185<\/span>TGN1412\u4e0e\u4eba\u514d\u75ab\u7ec6\u80de\u7684\u4f5c\u7528\u6a21\u5f0f\u3002<\/span><\/span><\/p>\n

\n
\n
\n
\n

\u5c55\u671b\uff1a<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

   <\/span><\/strong>\u9664\u4e86\u7ec6\u80de\u56e0\u5b50\u98ce\u66b4\u7684\u5b89\u5168\u6027\u6d4b\u5b9a\u5916\uff0c\u514d\u75ab\u8c03\u8282\u751f\u7269\u5236\u54c1\u7684\u4f53\u5916\u6d4b\u5b9a\u8fd8\u6709\u5f88\u591a\uff0c\u5982\u514d\u75ab\u539f\u6027\u7684\u6d4b\u5b9a\uff0c\u611f\u67d3\u548c\u5f15\u8d77\u6076\u6027\u80bf\u7624\u7684\u6d4b\u5b9a\uff0c\u6d89\u53ca\u5230\u5b9e\u9a8c\u6280\u672f\u548c\u5b9e\u9a8c\u65b9\u6848\u66f4\u662f\u591a\u79cd\u591a\u6837\u3002\u5728\u8fd9\u4e9b\u591a\u6837\u5316\u7684\u5b9e\u9a8c\u65b9\u6848\u4e2d\u5982\u4f55\u53bb\u8bc4\u5224\u7b80\u5355\u6761\u4ef6\u4e0b\u4f53\u5916\u7684\u514d\u75ab\u8c03\u8282\u751f\u7269\u5236\u54c1\u5b89\u5168\u6027\u6d4b\u5b9a\u7ed3\u679c\u548c\u590d\u6742\u60c5\u51b5\u4e0b\u4eba\u4f53\u5185\u751f\u7269\u5236\u54c1\u7684\u4f5c\u7528\u6a21\u5f0f\u7684\u5173\u8054\uff0c\u8fd9\u662f\u5f88\u96be\u8bf4\u6e05\u695a\u7684\u4e00\u4ef6\u4e8b\u60c5\u3002\u5c31TGN1412\u4f53\u5185\u4f53\u5916\u548c\u4e34\u5e8a\u7684\u5b9e\u9a8c\u7ed3\u679c\u800c\u8a00\uff0c\u5c0f\u7f16\u89c9\u90fd\u80fd\u4ece\u4e2d\u5438\u53d6\u7684\u7ecf\u9a8c\u6559\u8bad\u662f\uff1a\u5bf9\u514d\u75ab\u8c03\u8282\u751f\u7269\u5236\u54c1\u4eba\u4f53\u5185\u5b89\u5168\u6027\u7684\u9884\u6d4b\u6765\u6e90\u4e8e\u4f53\u5185\u52a8\u7269\u548c\u4f53\u5916\u6a21\u62df\u7684\u5b9e\u9a8c\u7ed3\u8bba\uff0c\u5b9e\u9a8c\u7ed3\u8bba\u6765\u6e90\u4e8e\u5b9e\u9a8c\u6570\u636e\uff0c\u800c\u5b9e\u9a8c\u6570\u636e\u6765\u6e90\u5b9e\u9a8c\u65b9\u6848\u7684\u5177\u4f53\u8bbe\u8ba1\uff0c\u5982\u4f55\u8bbe\u8ba1\u4e00\u4e2a\u4f53\u5916\u7684\u5b9e\u9a8c\u65b9\u6848\u8ba9\u5176\u80fd\u591f\u5c3d\u53ef\u80fd\u7684\u80fd\u591f\u6a21\u62df\u4eba\u4f53\u5185\u771f\u5b9e\u7684\u76f8\u4e92\u4f5c\u7528\u6a21\u5f0f\u662f\u9884\u6d4b\u6210\u529f\u7684\u5173\u952e\u3002<\/span><\/strong>\u8fd9\u4e2a\u5173\u952e\u9700\u8981\u6211\u4eec\u4ece\u6709\u9650\u7684\u4e34\u5e8a\u7ed3\u679c\u548c\u4f53\u5916\u7ed3\u679c\u7684\u5173\u8054\u4e2d\u53bb\u5438\u53d6\u7ecf\u9a8c\u6559\u8bad\uff0c\u5c31\u5982TGN1412\u6848\u4f8b\u4e2d\u4e00\u822c\uff0c\u540c\u65f6\u4e5f\u9700\u8981\u6211\u4eec\u5bf9\u5355\u4e00\u76ee\u7684\u7684\u5b9e\u9a8c\u65b9\u6848\u8fdb\u884c\u591a\u6837\u5316\u5b9e\u9a8c\u6761\u4ef6\u7684\u8bbe\u8ba1\uff0c\u5c31\u5982\u540c\u56de\u987e\u6027\u4f53\u5916\u6d4b\u5b9a\u4e2dP1-P6\u7684\u4f5c\u7528\u6a21\u5f0f\u4e00\u822c\uff0c\u5b89\u5168\u6027\u6d4b\u5b9a\u8981\u505a\u5168\u7684\u540c\u65f6\uff0c\u4e5f\u8981\u505a\u591a\uff0c\u505a\u5230\u7ec6\u81f4\u3002\u4e0d\u7ba1\u600e\u4e48\u8bf4\u65e0\u8bba\u662f\u4ece\u65f6\u95f4\u8fd8\u662f\u7ecf\u6d4e\u6210\u672c\u6765\u8bf4\uff0c\u4f53\u5916\u5b9e\u9a8c\u7684\u6210\u672c\u662f\u8fdc\u8fdc\u5c0f\u4e8e\u4e34\u5e8a\u6210\u672c\u7684\uff0c\u8fd9\u662f\u5bf9\u4ece\u4e1a\u8005\u4e13\u4e1a\u5316\u7a0b\u5ea6\u7684\u8981\u6c42\u3002<\/span><\/span><\/p>\n

\u53c2\u8003\u8d44\u6599\uff1a<\/span><\/strong><\/span><\/p>\n

Suntharalingam G, Perry M R, Ward S, et al. Cytokine Storm in a Phase 1 Trial of the Anti-CD28 Monoclonal Antibody TGN1412[J]. N Engl J Med, 2006, 355(10):1018-1028.<\/span><\/span><\/p>\n

\u8bc6\u522b\u5fae\u4fe1\u4e8c\u7ef4\u7801\uff0c\u6dfb\u52a0\u751f\u7269\u5236\u54c1\u5708\u5c0f\u7f16\uff0c\u7b26\u5408\u6761\u4ef6\u8005\u5373\u53ef\u52a0\u5165\u751f\u7269\u5236\u54c1\u5fae\u4fe1\u7fa4\uff01<\/strong>
<\/p>\n

\n
\n

\u8bf7\u6ce8\u660e\uff1a\u59d3\u540d+\u7814\u7a76\u65b9\u5411\uff01<\/strong><\/span><\/p>\n

\"Case<\/p>\n

\n
\n
\n

<\/section>\n<\/section>\n<\/section>\n<\/section>\n
\n
\n
\n
<\/section>\n
<\/section>\n<\/section>\n
\n
\n
<\/span><\/strong><\/span>\u7248<\/section>\n<\/section>\n
\n
\u6743<\/section>\n<\/section>\n
\n
\u58f0<\/section>\n<\/section>\n
\n
\u660e<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

\u7248\u6743\u4e3a\u751f\u7269\u5236\u54c1\u5708\u6240\u6709\u3002\u6b22\u8fce\u4e2a\u4eba\u8f6c\u53d1\u5206\u4eab\u3002\u5176\u4ed6\u4efb\u4f55\u5a92\u4f53\u3001\u7f51\u7ad9\u5982\u9700\u8f6c\u8f7d\u6216\u5f15\u7528\u672c\u7f51\u7248\u6743\u6240\u6709\u5185\u5bb9\u987b\u83b7\u5f97\u6388\u6743\u4e14\u5728\u9192\u76ee\u4f4d\u7f6e\u5904\u6ce8\u660e\u201c\u8f6c\u81ea\uff1a\u751f\u7269\u5236\u54c1\u5708\u201d\u3002\"Case<\/span><\/p>\n<\/section>\n<\/section>\n

\n

\u59cb\u53d1\u4e8e\u5fae\u4fe1\u516c\u4f17\u53f7\uff1a \u751f\u7269\u5236\u54c1\u5708 <\/p>\n<\/blockquote>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

\u524d\u8a00\uff1a\u968f\u7740PD-1\u6297\u4f53\u9002\u5e94\u75c7\u7684\u4e0d\u65ad\u6269\u5c55\uff0c\u56fd\u5916O\u836f\u548cK\u836f\u76f8\u7ee7\u5728\u4e2d\u56fd\u7533\u8bf7\u4e0a\u5e02\uff0c\u56fd\u5185\u4fe1\u8fbe\uff0c\u541b\u5b9e\u548c\u6052\u745e\u4e89\u53d6PD-1\u6297\u4f53 […]<\/p>\n","protected":false},"author":1,"featured_media":25547,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[2],"tags":[],"_links":{"self":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/25546"}],"collection":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/comments?post=25546"}],"version-history":[{"count":1,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/25546\/revisions"}],"predecessor-version":[{"id":25562,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/25546\/revisions\/25562"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media\/25547"}],"wp:attachment":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media?parent=25546"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/categories?post=25546"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/tags?post=25546"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}