{"id":30961,"date":"2018-08-03T01:00:00","date_gmt":"2018-08-09T04:12:41","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/2018\/08\/09\/ctdna%e6%a3%80%e6%b5%8b%e7%9a%84%e7%a7%98%e5%af%86%e6%ad%a6%e5%99%a8-duplex-umi-ctdna%e6%b5%8b%e5%ba%8f%e6%8a%80%e6%9c%af\/"},"modified":"2018-08-09T12:12:43","modified_gmt":"2018-08-09T04:12:43","slug":"ctdna%e6%a3%80%e6%b5%8b%e7%9a%84%e7%a7%98%e5%af%86%e6%ad%a6%e5%99%a8-duplex-umi-ctdna%e6%b5%8b%e5%ba%8f%e6%8a%80%e6%9c%af","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/30961\/","title":{"rendered":"ctDNA\u68c0\u6d4b\u7684\u79d8\u5bc6\u6b66\u5668\u2014Duplex UMI-ctDNA\u6d4b\u5e8f\u6280\u672f"},"content":{"rendered":"<div class=\"bpp-post-content\">\n<p><img class=\"\"       style=\"\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/08\/beepress10-1533787962.jpeg\" alt=\"ctDNA\u68c0\u6d4b\u7684\u79d8\u5bc6\u6b66\u5668\u2014Duplex UMI-ctDNA\u6d4b\u5e8f\u6280\u672f\" title=\"ctDNA\u68c0\u6d4b\u7684\u79d8\u5bc6\u6b66\u5668\u2014Duplex UMI-ctDNA\u6d4b\u5e8f\u6280\u672f\"  \/><\/p>\n<section class=\"\"  style=\"max-width: 100%;box-sizing: border-box;\">\n<section style=\"margin-top: 0.5em;margin-bottom: 0.5em;max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;display: inline-block;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;display: inline-block;width: 1.5em;height: 1.5em;border-radius: 100%;vertical-align: bottom;background-color: rgb(67, 83, 108);color: rgb(255, 255, 255);text-align: center;font-size: 32px;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">1<\/section>\n<\/section>\n<section style=\"margin-top: -2.3em;margin-bottom: 0.8em;margin-left: 2em;padding-right: 5px;max-width: 100%;box-sizing: border-box;background-color: rgb(185, 238, 228);word-wrap: break-word !important;\">\n<section style=\"padding-right: 10px;padding-left: 20px;max-width: 100%;box-sizing: border-box;background-color: rgb(205, 220, 241);color: rgb(0, 0, 0);text-align: center;word-wrap: break-word !important;\">\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;word-wrap: break-word !important;\">\u6db2\u4f53\u6d3b\u68c0\u5f25\u8865\u7ec4\u7ec7\u68c0\u6d4b\u7684\u4e0d\u8db3<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"\"  style=\"max-width: 100%;box-sizing: border-box;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;width: 710px;line-height: 1.5em;background-color: rgb(241, 241, 241);word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;width: 710px;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;width: 0.62em;height: 0.62em;float: left;background-color: rgb(87, 209, 249);word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;width: 0.62em;height: 0.62em;float: right;background-color: rgb(87, 209, 249);word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;clear: both;word-wrap: break-word !important;\"><\/section>\n<\/section>\n<section style=\"padding: 10px 5px;max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section class=\"\"  style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;font-size: 14px;line-height: 2;word-wrap: break-word !important;\">\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;text-indent: 2em;word-wrap: break-word !important;\">\u540c\u4e00\u764c\u79cd\u7684\u4e0d\u540c\u60a3\u8005\uff0c\u540c\u4e00\u4e2a\u80bf\u7624\u60a3\u8005\u7684\u4e0d\u540c\u6cbb\u7597\u9636\u6bb5\u3001\u540c\u4e00\u80bf\u7624\u7ec4\u7ec7\u7684\u4e0d\u540c\u533a\u57df\uff0c\u80bf\u7624\u7684\u751f\u7269\u5b66\u7279\u5f81\u5747\u5b58\u5728\u4e00\u5b9a\u7684\u5dee\u5f02\u3002\u6db2\u4f53\u6d3b\u68c0\u65e2\u53ef\u4ee5\u514b\u670d\u7ec4\u7ec7\u68c0\u6d4b\u7684\u5f02\u8d28\u6027\uff0c\u53c8\u5177\u6709\u7b80\u4fbf\u3001\u5b89\u5168\u3001\u65e0\u521b\u3001\u5b9e\u65f6\u7b49\u7279\u70b9\uff0c\u5c24\u5176\u662f\u5bf9\u4e8e\u65e0\u6cd5\u8fdb\u884c\u624b\u672f\u6216\u7a7f\u523a\uff0c\u53c8\u6216\u8005\u80bf\u7624\u4f4d\u7f6e\u5bfc\u81f4\u53d6\u6837\u56f0\u96be\u7684\u60a3\u8005\u800c\u8a00\uff0c\u6db2\u4f53\u6d3b\u68c0\u662f\u4e00\u9879\u53ef\u4ee5\u5f25\u8865\u7ec4\u7ec7\u68c0\u6d4b\u5c40\u9650\u6027\u7684\u65b9\u6cd5\uff0c\u8fd1\u5e74\u6765\u5728\u80bf\u7624\u9776\u5411\u6cbb\u7597\u3001\u8010\u836f\u76d1\u6d4b\u7684\u5b9e\u65f6\u8bc4\u4f30\u7b49\u65b9\u9762\u53d1\u6325\u7740\u91cd\u8981\u7684\u4f5c\u7528\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;width: 710px;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;width: 0.62em;height: 0.62em;float: left;background-color: rgb(87, 209, 249);word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;width: 0.62em;height: 0.62em;float: right;background-color: rgb(87, 209, 249);word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;clear: both;word-wrap: break-word !important;\"><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"\"  style=\"max-width: 100%;box-sizing: border-box;\">\n<section style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\"  \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"\"  style=\"max-width: 100%;box-sizing: border-box;\">\n<section style=\"margin-top: 0.5em;margin-bottom: 0.5em;max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;display: inline-block;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;display: inline-block;width: 1.5em;height: 1.5em;border-radius: 100%;vertical-align: bottom;background-color: rgb(67, 83, 108);color: rgb(255, 255, 255);text-align: center;font-size: 32px;word-wrap: break-word !important;\">\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;word-wrap: break-word !important;\">2<\/p>\n<\/section>\n<section style=\"margin-top: -2.3em;margin-bottom: 0.8em;margin-left: 2em;padding-right: 5px;max-width: 100%;box-sizing: border-box;background-color: rgb(185, 238, 228);word-wrap: break-word !important;\">\n<section style=\"padding-right: 10px;padding-left: 20px;max-width: 100%;box-sizing: border-box;background-color: rgb(205, 220, 241);color: rgb(0, 0, 0);text-align: center;word-wrap: break-word !important;\">\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;word-wrap: break-word !important;\">\u4ec0\u4e48\u662fctDNA\u68c0\u6d4b\uff1f<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"\"  style=\"max-width: 100%;box-sizing: border-box;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;width: 710px;line-height: 1.5em;background-color: rgb(241, 241, 241);word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;width: 710px;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;width: 0.62em;height: 0.62em;float: left;background-color: rgb(87, 209, 249);word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;width: 0.62em;height: 0.62em;float: right;background-color: rgb(87, 209, 249);word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;clear: both;word-wrap: break-word !important;\"><\/section>\n<\/section>\n<section style=\"padding: 10px 5px;max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section class=\"\"  style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;font-size: 14px;line-height: 2;word-wrap: break-word !important;\">\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;text-indent: 2em;word-wrap: break-word !important;\">\u76ee\u524d\u6db2\u4f53\u6d3b\u68c0\u7684\u9776\u6807\u5305\u62ec\uff1a\u6e38\u79bb\u7684\u5faa\u73af\u80bf\u7624\u7ec6\u80de\uff08CTCs\uff09\u3001\u5faa\u73af\u80bf\u7624DNA\uff08ctDNA\uff09\u3001\u5faa\u73af\u80bf\u7624RNA\uff08ctRNA\uff09\u548c\u5916\u6ccc\u4f53\uff08\u643a\u5e26\u6709\u7ec6\u80de\u6765\u6e90\u76f8\u5173\u7684\u591a\u79cd\u86cb\u767d\u8d28\uff0c\u8102\u7c7b\uff0cDNA\uff0cRNA\u7b49\uff09\u3002<\/p>\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;text-indent: 2em;word-wrap: break-word !important;\">ctDNA\u662f\u80bf\u7624\u7ec6\u80de\u5728\u574f\u6b7b\u3001\u51cb\u4ea1\u540e\u91ca\u653e\u7684\u4e00\u79cd\u6e38\u79bbDNA\uff08cfDNA\uff09\uff0c\u5728\u8840\u6db2\u4e2d\u7684\u534a\u8870\u671f\u77ed\uff0c\u53ef\u4ee5\u5b9e\u65f6\u53cd\u6620\u80bf\u7624\u7684\u52a8\u6001\u53d8\u5316\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;width: 710px;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;width: 0.62em;height: 0.62em;float: left;background-color: rgb(87, 209, 249);word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;width: 0.62em;height: 0.62em;float: right;background-color: rgb(87, 209, 249);word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;clear: both;word-wrap: break-word !important;\"><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"\"  style=\"max-width: 100%;box-sizing: border-box;\">\n<section style=\"max-width: 100%;box-sizing: border-box;text-align: center;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;display: inline-block;vertical-align: middle;word-wrap: break-word !important;\"><img    class=\"\"  style=\"box-sizing: border-box;border-width: 0px;border-style: initial;border-color: initial;vertical-align: middle;word-wrap: break-word !important;width: auto !important;visibility: visible !important;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/08\/beepress7-1533787962.jpg\" alt=\"ctDNA\u68c0\u6d4b\u7684\u79d8\u5bc6\u6b66\u5668\u2014Duplex UMI-ctDNA\u6d4b\u5e8f\u6280\u672f\" title=\"ctDNA\u68c0\u6d4b\u7684\u79d8\u5bc6\u6b66\u5668\u2014Duplex UMI-ctDNA\u6d4b\u5e8f\u6280\u672f\"  \/><\/section>\n<\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;text-align: center;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;display: inline-block;vertical-align: middle;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/section>\n<\/section>\n<\/section>\n<section class=\"\"  style=\"max-width: 100%;box-sizing: border-box;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;width: 710px;line-height: 1.5em;background-color: rgb(241, 241, 241);word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;width: 710px;word-wrap: break-word !important;\"><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"\"  style=\"max-width: 100%;box-sizing: border-box;\">\n<section style=\"margin-top: 0.5em;margin-bottom: 0.5em;max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;display: inline-block;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;display: inline-block;width: 1.5em;height: 1.5em;border-radius: 100%;vertical-align: bottom;background-color: rgb(67, 83, 108);color: rgb(255, 255, 255);text-align: center;font-size: 32px;word-wrap: break-word !important;\">\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;word-wrap: break-word !important;\">3<\/p>\n<\/section>\n<section style=\"margin-top: -2.3em;margin-bottom: 0.8em;margin-left: 2em;padding-right: 5px;max-width: 100%;box-sizing: border-box;background-color: rgb(185, 238, 228);word-wrap: break-word !important;\">\n<section style=\"padding-right: 10px;padding-left: 20px;max-width: 100%;box-sizing: border-box;background-color: rgb(205, 220, 241);color: rgb(0, 0, 0);text-align: center;word-wrap: break-word !important;\">\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;word-wrap: break-word !important;\">\u4e3a\u4ec0\u4e48ctDNA\u68c0\u6d4b\u9700\u8981\u66f4\u9ad8\u7684\u7075\u654f\u5ea6\uff1f<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"\"  style=\"max-width: 100%;box-sizing: border-box;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;width: 710px;line-height: 1.5em;background-color: rgb(241, 241, 241);word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;width: 710px;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;width: 0.62em;height: 0.62em;float: left;background-color: rgb(87, 209, 249);word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;width: 0.62em;height: 0.62em;float: right;background-color: rgb(87, 209, 249);word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;clear: both;word-wrap: break-word !important;\"><\/section>\n<\/section>\n<section style=\"padding: 10px 5px;max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section class=\"\"  style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;font-size: 14px;line-height: 2;word-wrap: break-word !important;\">\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;text-indent: 2em;word-wrap: break-word !important;\"><span style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\u80bf\u7624\u60a3\u8005\u4f53\u5185\u7684\u80bf\u7624\u7ec6\u80de\u6570\u91cf\u8fdc\u8fdc\u4f4e\u4e8e\u6b63\u5e38\u7ec6\u80de\uff0ccfDNA\u5728\u8840\u6d46\u4e2d\u7684\u542b\u91cf\u5f88\u4f4e\uff0c\u800cctDNA\u4ec5\u5360cfDNA\u76840.1%~5%\uff0c\u4e14\u4e0d\u540c\u764c\u79cd\uff0c\u4e0d\u540c\u75c5\u7a0b\u7684\u80bf\u7624\u60a3\u8005ctDNA\u5728\u8840\u6d46\u4e2d\u542b\u91cf\u5dee\u5f02\u8f83\u5927\uff0c\u56e0\u6b64\u76f8\u6bd4\u4e8e\u7ec4\u7ec7\u68c0\u6d4b\uff0cctDNA\u7684\u68c0\u6d4b\u9700\u8981\u66f4\u9ad8\u7684\u7075\u654f\u5ea6\u548c\u7279\u5f02\u6027\u3002<\/span><\/p>\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;text-indent: 2em;word-wrap: break-word !important;\">\u76ee\u524d\u7528\u4e8ectDNA\u6db2\u4f53\u6d3b\u68c0\u7684\u6280\u672f\u4e3b\u8981\u6709ARMS-PCR\u3001\u6570\u5b57PCR\uff08ddPCR\uff09\u548c\u7b2c\u4e8c\u4ee3\u6d4b\u5e8f\uff08NGS\uff09\u3002<\/p>\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;text-indent: 2em;word-wrap: break-word !important;\">NGS\u80fd\u540c\u65f6\u68c0\u6d4b\u591a\u4e2a\u57fa\u56e0\u7684\u591a\u79cd\u4e0d\u540c\u53d8\u5f02\u5f62\u5f0f\uff0c\u662f\u5e94\u7528\u6700\u5e7f\u6cdb\u7684\u7684\u57fa\u56e0\u68c0\u6d4b\u6280\u672f\u3002\u4f46\u7531\u4e8eNGS\u7684\u5b9e\u9a8c\u6d41\u7a0b\u6280\u672f\u8f83\u4e3a\u590d\u6742\uff0c\u5728\u6587\u5e93\u6784\u5efa\u3001\u76ee\u6807\u533a\u57df\u6355\u83b7\u53ca\u6d4b\u5e8f\u8fc7\u7a0b\u4e2d\u4e0d\u53ef\u907f\u514d\u7684\u4f1a\u5f15\u5165\u4e00\u4e9b\u6269\u589e\u548c\u6d4b\u5e8f\u7684\u9519\u8bef\uff0c\u8fd9\u4e9b\u9519\u8bef\u6211\u4eec\u628a\u5b83\u4eec\u53eb\u505a\u80cc\u666f\u566a\u97f3\uff0c\u800cctDNA\u68c0\u6d4b\u5f80\u5f80\u7a81\u53d8\u9891\u7387\u8f83\u4f4e\uff0c\u53d7\u5230\u80cc\u666f\u566a\u97f3\u5e72\u6270\u8f83\u5927\uff0c\u6765\u81eactDNA\u6837\u672c\u4e2d\u7684\u4f4e\u9891\u7a81\u53d8\u5f80\u5f80\u6df9\u6ca1\u5728\u80cc\u666f\u566a\u97f3\u4e4b\u4e2d\uff0c\u9020\u6210\u5047\u9634\u6027\u6216\u5047\u9633\u6027\u7ed3\u679c\uff0c\u8fd9\u5c31\u9650\u5236\u4e86ctDNA\u68c0\u6d4b\u7684\u7075\u654f\u5ea6\u548c\u7279\u5f02\u6027\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;width: 710px;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;width: 0.62em;height: 0.62em;float: left;background-color: rgb(87, 209, 249);word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;width: 0.62em;height: 0.62em;float: right;background-color: rgb(87, 209, 249);word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;clear: both;word-wrap: break-word !important;\"><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"\"  style=\"max-width: 100%;box-sizing: border-box;\">\n<section style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\"  \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"\"  style=\"max-width: 100%;box-sizing: border-box;\">\n<section style=\"margin-top: 0.5em;margin-bottom: 0.5em;max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;display: inline-block;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;display: inline-block;width: 1.5em;height: 1.5em;border-radius: 100%;vertical-align: bottom;background-color: rgb(67, 83, 108);color: rgb(255, 255, 255);text-align: center;font-size: 32px;word-wrap: break-word !important;\">\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;word-wrap: break-word !important;\">4<\/p>\n<\/section>\n<section style=\"margin-top: -2.3em;margin-bottom: 0.8em;margin-left: 2em;padding-right: 5px;max-width: 100%;box-sizing: border-box;background-color: rgb(185, 238, 228);word-wrap: break-word !important;\">\n<section style=\"padding-right: 10px;padding-left: 20px;max-width: 100%;box-sizing: border-box;background-color: rgb(205, 220, 241);color: rgb(0, 0, 0);text-align: center;word-wrap: break-word !important;\">\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;word-wrap: break-word !important;\">\u5982\u4f55\u63d0\u9ad8ctDNA\u68c0\u6d4b\u7684\u7075\u654f\u5ea6\u548c\u7279\u5f02\u6027\uff1f<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"\"  style=\"max-width: 100%;box-sizing: border-box;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;width: 710px;line-height: 1.5em;background-color: rgb(241, 241, 241);word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;width: 710px;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;width: 0.62em;height: 0.62em;float: left;background-color: rgb(87, 209, 249);word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;width: 0.62em;height: 0.62em;float: right;background-color: rgb(87, 209, 249);word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;clear: both;word-wrap: break-word !important;\"><\/section>\n<\/section>\n<section style=\"padding: 10px 5px;max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section class=\"\"  style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;font-size: 14px;line-height: 2;word-wrap: break-word !important;\">\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;text-indent: 2em;word-wrap: break-word !important;\"><span style=\"max-width: 100%;color: rgb(255, 76, 0);box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\u90a3\u5c31\u4e0d\u5f97\u4e0d\u63d0\u5230\u4e00\u9879\u9887\u5177\u524d\u666f\u7684\u6280\u672f\u2014\u5206\u5b50\u6761\u5f62\u7801\u6280\u672f<\/strong><\/span><\/p>\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;text-indent: 2em;word-wrap: break-word !important;\">\u5206\u5b50\u6761\u5f62\u7801\u53c8\u79f0\u5206\u5b50\u6807\u7b7e\uff08Unique Molecular indentifier\uff0cUMI\uff09\uff0c\u5b83\u7684\u539f\u7406\u5c31\u662f\u7ed9\u6bcf\u4e00\u6761\u539f\u59cbDNA\u7247\u6bb5\u52a0\u4e0a\u4e00\u6bb5\u7279\u6709\u7684\u6807\u7b7e\u5e8f\u5217\uff0c\u7ecf\u6587\u5e93\u6784\u5efa\u53caPCR\u6269\u589e\u540e\u4e00\u8d77\u8fdb\u884c\u6d4b\u5e8f\u3002\u8fd9\u6837\uff0c\u6839\u636e\u4e0d\u540c\u7684\u6807\u7b7e\u5e8f\u5217\u6211\u4eec\u5c31\u53ef\u4ee5\u533a\u5206\u4e0d\u540c\u6765\u6e90\u7684DNA\u6a21\u677f\uff0c\u5206\u8fa8\u54ea\u4e9b\u662fPCR\u6269\u589e\u53ca\u6d4b\u5e8f\u8fc7\u7a0b\u4e2d\u7684\u968f\u673a\u9519\u8bef\u9020\u6210\u7684\u5047\u9633\u6027\u7a81\u53d8\uff0c\u54ea\u4e9b\u662f\u60a3\u8005\u771f\u6b63\u7684\u643a\u5e26\u7684\u7a81\u53d8\uff0c\u4ece\u800c\u63d0\u9ad8\u68c0\u6d4b\u7075\u654f\u5ea6\u548c\u7279\u5f02\u6027\u3002<\/p>\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;text-indent: 2em;word-wrap: break-word !important;\">\u2460\u30002012\u5e745\u6708\uff0cTim Forshew\u7b49\u4eba\u7387\u5148\u5f00\u53d1\u4e86\u57fa\u4e8e\u5206\u5b50\u6807\u7b7e\u7684TAm-Seq\u65b9\u6cd5\u3002<\/p>\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;text-indent: 2em;word-wrap: break-word !important;\">\u2461\u30002012\u5e749\u6708\uff0cMichael W. Schmitt\u7b49\u5229\u7528\u53cc\u94fe\u5206\u5b50\u6807\u7b7e\u6280\u672f\u5c06\u9519\u8bef\u7387\u663e\u8457\u964d\u4f4e\u3002\u5b83\u7684\u6280\u672f\u539f\u7406\u5982\u4e0b\uff1a<\/p>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"\"  style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;max-width: 100%;box-sizing: border-box;text-align: center;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;display: inline-block;vertical-align: middle;word-wrap: break-word !important;\"><img    class=\"\"  style=\"box-sizing: border-box;background-color: rgb(238, 237, 235);border-width: 0px;border-style: initial;border-color: initial;background-size: 22px;background-position: center center;background-repeat: no-repeat;vertical-align: middle;height: 426px !important;word-wrap: break-word !important;width: 370px !important;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/08\/beepress2-1533787962.jpg\" alt=\"ctDNA\u68c0\u6d4b\u7684\u79d8\u5bc6\u6b66\u5668\u2014Duplex UMI-ctDNA\u6d4b\u5e8f\u6280\u672f\" title=\"ctDNA\u68c0\u6d4b\u7684\u79d8\u5bc6\u6b66\u5668\u2014Duplex UMI-ctDNA\u6d4b\u5e8f\u6280\u672f\"  \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;width: 710px;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;width: 0.62em;height: 0.62em;float: left;background-color: rgb(87, 209, 249);word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;width: 0.62em;height: 0.62em;float: right;background-color: rgb(87, 209, 249);word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;clear: both;word-wrap: break-word !important;\"><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"\"  style=\"max-width: 100%;box-sizing: border-box;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;width: 710px;line-height: 1.5em;background-color: rgb(241, 241, 241);word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;width: 710px;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;width: 0.62em;height: 0.62em;float: left;background-color: rgb(87, 209, 249);word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;width: 0.62em;height: 0.62em;float: right;background-color: rgb(87, 209, 249);word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;clear: both;word-wrap: break-word !important;\"><\/section>\n<\/section>\n<section style=\"padding: 10px 5px;max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section class=\"\"  style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;font-size: 14px;line-height: 2;word-wrap: break-word !important;\">\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;text-indent: 2em;word-wrap: break-word !important;\">\u5982\u56fe\u4e2d\u6240\u793a\uff0c\u03b1\u548c\u03b2\u5747\u662f\u8fde\u63a5\u5230DNA\u6a21\u677f\u4e0a\u7684\u968f\u673aDNA\u5e8f\u5217\uff0c\u4e0d\u540c\u7684\u6a21\u677f\u8fde\u63a5\u7684\u5e8f\u5217\u4e0d\u540c\uff0c\u5373\u4e3actDNA\u52a0\u4e0a\u4e86\u72ec\u7279\u7684\u6807\u8bb0\u3002\u7ecfPCR\u6269\u589e\u540e\u901a\u8fc7\u03b1\u548c\u03b2\u7684\u5e8f\u5217\u5c31\u53ef\u4ee5\u8bc6\u522b\u51fa\u540c\u4e00\u6a21\u677f\u6765\u6e90\u7684\u7247\u6bb5\uff0c\u518d\u6839\u636e\u03b1\u548c\u03b2\u4e92\u8865\u8bc6\u522b\u540c\u4e00\u6a21\u677f\u7684\u6b63\u53cd\u94fe\u3002<\/p>\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;text-indent: 2em;word-wrap: break-word !important;\">\u2460\u3000i\u56fe\u4e2d\u4e00\u4e2a\u9519\u8bef\u4ec5\u5f71\u54cd\u4e86\u6269\u589e\u51fa\u7684\u5c11\u6570\u5e8f\u5217\uff0c\u7edf\u4e00\u5206\u6790\u540c\u4e00\u6a21\u677f\u6765\u6e90\u7684\u7247\u6bb5\u53ef\u4ee5\u5c06\u8fd9\u4e2a\u9519\u8bef\u8fc7\u6ee4\uff1b<\/p>\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;text-indent: 2em;word-wrap: break-word !important;\">\u2461\u3000ii\u56fe\u4e2d\u51fa\u73b0\u7684\u9519\u8bef\u5f71\u54cd\u4e86\u6240\u6709\u6b63\u94fe\u5e8f\u5217\uff0c\u4f46\u53cd\u94fe\u6b63\u5e38\uff0c\u6839\u636e\u78b1\u57fa\u4e92\u8865\u539f\u5219\uff0c\u53ef\u5c06\u6b64\u79cd\u9519\u8bef\u6821\u6b63\uff1b<\/p>\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;text-indent: 2em;word-wrap: break-word !important;\">\u2462\u3000iii\u56fe\u4e2d\u5219\u663e\u793a\u4e86\u6392\u9664\u4e86\u4e00\u4e9b\u5f15\u5165\u9519\u8bef\u548c\u7cfb\u7edf\u9519\u8bef\u540e\u5f97\u5230\u771f\u6b63\u7684\u7a81\u53d8\u3002<\/p>\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;text-indent: 2em;word-wrap: break-word !important;\">2015\u5e748\u6708\uff0cPeng\u7b49\u4eba\u7684\u7814\u7a76\u4e2d\u5229\u7528\u5206\u5b50\u6761\u5f62\u7801\u4e0e\u4e0d\u7528\u5206\u5b50\u6761\u5f62\u7801\u7684\u5b9e\u9a8c\u8fdb\u884c\u5bf9\u6bd4\uff0c\u901a\u8fc7\u5bf9\u5b9e\u9a8c\u6570\u636e\u7684\u5206\u6790\u53d1\u73b0\u901a\u8fc7\u5f15\u5165\u5206\u5b50\u6761\u5f62\u7801\u6280\u672f\uff0c\u5927\u5927\u964d\u4f4e\u4e86\u4f4e\u9891\u7a81\u53d8\u7684\u5047\u9633\u6027\u7387\u3002<\/p>\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;text-indent: 2em;word-wrap: break-word !important;\">2016\u5e745\u6708\uff0c\u65af\u5766\u798f\u5927\u5b66\u533b\u5b66\u9662\u7684\u7814\u7a76\u4eba\u5458\u57fa\u4e8e\u5176\u66fe\u5f00\u53d1\u7684ctDNA\u5b9a\u91cf\u68c0\u6d4b\u65b9\u6cd5Capp-Seq\u6cd5\uff0c\u53c8\u5f15\u5165\u4e86\u5206\u5b50\u6761\u5f62\u7801\u7b56\u7565\u548c\u96c6\u6210\u6570\u5b57\u9519\u8bef\u6291\u5236\u65b9\u6cd5\uff08iDES\uff09\uff0c\u4f7f\u6d4b\u5e8f\u7075\u654f\u5ea6\u6709\u4e86\u5f88\u5927\u7684\u63d0\u9ad8\u3002<\/p>\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;text-indent: 0em;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"\"  style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;max-width: 100%;box-sizing: border-box;text-align: center;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;display: inline-block;vertical-align: middle;word-wrap: break-word !important;\"><img    class=\"\"  style=\"box-sizing: border-box;border-width: 0px;border-style: initial;border-color: initial;vertical-align: middle;word-wrap: break-word !important;width: auto !important;visibility: visible !important;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/08\/beepress0-1533787962.jpg\" alt=\"ctDNA\u68c0\u6d4b\u7684\u79d8\u5bc6\u6b66\u5668\u2014Duplex UMI-ctDNA\u6d4b\u5e8f\u6280\u672f\" title=\"ctDNA\u68c0\u6d4b\u7684\u79d8\u5bc6\u6b66\u5668\u2014Duplex UMI-ctDNA\u6d4b\u5e8f\u6280\u672f\"  \/><\/section>\n<\/section>\n<\/section>\n<section class=\"\"  style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;max-width: 100%;box-sizing: border-box;text-align: center;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;display: inline-block;vertical-align: middle;word-wrap: break-word !important;\"><img    class=\"\"  style=\"box-sizing: border-box;border-width: 0px;border-style: initial;border-color: initial;vertical-align: middle;word-wrap: break-word !important;width: auto !important;visibility: visible !important;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/08\/beepress6-1533787963.jpg\" alt=\"ctDNA\u68c0\u6d4b\u7684\u79d8\u5bc6\u6b66\u5668\u2014Duplex UMI-ctDNA\u6d4b\u5e8f\u6280\u672f\" title=\"ctDNA\u68c0\u6d4b\u7684\u79d8\u5bc6\u6b66\u5668\u2014Duplex UMI-ctDNA\u6d4b\u5e8f\u6280\u672f\"  \/><\/section>\n<\/section>\n<\/section>\n<section class=\"\"  style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;max-width: 100%;box-sizing: border-box;text-align: center;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;display: inline-block;vertical-align: middle;word-wrap: break-word !important;\"><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;width: 710px;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;width: 0.62em;height: 0.62em;float: left;background-color: rgb(87, 209, 249);word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;width: 0.62em;height: 0.62em;float: right;background-color: rgb(87, 209, 249);word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;clear: both;word-wrap: break-word !important;\"><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"\"  style=\"max-width: 100%;box-sizing: border-box;\">\n<section style=\"margin-top: 10px;margin-bottom: 10px;max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;width: 710px;line-height: 1.5em;background-color: rgb(241, 241, 241);word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;width: 710px;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;width: 0.62em;height: 0.62em;float: left;background-color: rgb(87, 209, 249);word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;width: 0.62em;height: 0.62em;float: right;background-color: rgb(87, 209, 249);word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;clear: both;word-wrap: break-word !important;\"><\/section>\n<\/section>\n<section style=\"padding: 10px 5px;max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section class=\"\"  style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;font-size: 14px;line-height: 2;word-wrap: break-word !important;\">\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;text-indent: 2em;word-wrap: break-word !important;\">\u7814\u7a76\u4eba\u5458\u53d1\u73b0\uff0c\u4e24\u79cd\u6280\u672f\u7ed3\u5408\u4f7f\u7528\u4f7f\u9519\u8bef\u7387\u4e0b\u964d\u4e8615\u500d\u3002\u901a\u8fc7\u8fd9\u79cd\u65b9\u6cd5\u6765\u76d1\u63a730\u540dNSCLC\u60a3\u8005\u4e2d\u7684\u7a81\u53d8\uff0c\u5bf9EGFR\u6fc0\u9176\u57df\u7a81\u53d8\u7684\u654f\u611f\u5ea6\u8fbe\u523092%\uff0c\u7279\u5f02\u6027\u8fbe96%\uff0c\u5e76\u80fd\u591f\u68c0\u6d4b\u5230\u9891\u7387\u4f4e\u81f30.004%\u7684\u7a81\u53d8\u3002<\/p>\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;text-indent: 2em;word-wrap: break-word !important;\">\u901a\u8fc7\u4ee5\u4e0a\u8fd9\u4e9b\u7814\u7a76\u62a5\u9053\u53ef\u4ee5\u770b\u51fa\uff0c\u5206\u5b50\u6807\u7b7e\u6280\u672f\u4f7f\u5f97NGS\u6280\u672f\u7684\u7075\u654f\u5ea6\u548c\u7279\u5f02\u6027\u90fd\u5f97\u5230\u4e86\u5f88\u5927\u7684\u63d0\u9ad8\uff0c\u8fd9\u4e3a\u5176\u5728\u80bf\u7624\u65e9\u671f\u8bca\u65ad\u3001\u7528\u836f\u65b9\u9762\u7684\u6307\u5bfc\u4ee5\u53ca\u8010\u836f\u673a\u5236\u5206\u6790\u7b49\u65b9\u9762\u7684\u5e94\u7528\u521b\u9020\u51fa\u8d8a\u6765\u8d8a\u5927\u7684\u4f18\u52bf\u3002<\/p>\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;text-indent: 2em;word-wrap: break-word !important;\"><span style=\"max-width: 100%;color: rgb(255, 41, 65);box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\u6676\u6cf0\u901a\u8fc7\u4e0d\u65ad\u4f18\u5316\uff0c\u57fa\u4e8e\u53cc\u94fe\u5206\u5b50\u6807\u7b7e\u7684\u539f\u7406\uff0c\u5f00\u53d1\u51faDuplex UMI-ctDNA\u6d4b\u5e8f\u6280\u672f\u3002<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;width: 710px;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;width: 0.62em;height: 0.62em;float: left;background-color: rgb(87, 209, 249);word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;width: 0.62em;height: 0.62em;float: right;background-color: rgb(87, 209, 249);word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;clear: both;word-wrap: break-word !important;\"><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section class=\"\"  style=\"max-width: 100%;box-sizing: border-box;\">\n<section style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;font-size: 14px;line-height: 2;word-wrap: break-word !important;\">\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;word-wrap: break-word !important;\"><span style=\"max-width: 100%;color: rgb(178, 178, 178);box-sizing: border-box !important;word-wrap: break-word !important;\">\u53c2\u8003\u6587\u732e\uff1a<\/span><\/p>\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;word-wrap: break-word !important;\"><span style=\"max-width: 100%;color: rgb(178, 178, 178);box-sizing: border-box !important;word-wrap: break-word !important;\">[1] Schmitt M W, Kennedy S R, Salk J J, et al. Detection of ultra-rare mutations by next-generation sequencing[J]. Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(36):14508-14513.<\/span><\/p>\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;word-wrap: break-word !important;\"><span style=\"max-width: 100%;color: rgb(178, 178, 178);box-sizing: border-box !important;word-wrap: break-word !important;\">[2] Penget al. Reducing amplification artifacts in high multiplex amplicon sequencingby using molecular barcodes. BMC Genomics. 2015 Aug 7;16:589.<\/span><\/p>\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;word-wrap: break-word !important;\"><span style=\"max-width: 100%;color: rgb(178, 178, 178);box-sizing: border-box !important;word-wrap: break-word !important;\">[3] Newman A M, Lovejoy A F, Klass D M, et al. Integrated digital error suppression for improved detection of circulating tumor DNA[J]. Nature Biotechnology, 2016, 34(5):547.<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<p><span style=\"color: rgb(136, 136, 136);\"><strong>\u6765\u6e90\uff1a<span style=\";\">\u6676\u6cf0\u533b\u5b66<\/span><\/strong><\/span><\/p>\n<p><img class=\"\"       style=\"\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/08\/beepress1-1533787963.jpeg\" alt=\"ctDNA\u68c0\u6d4b\u7684\u79d8\u5bc6\u6b66\u5668\u2014Duplex UMI-ctDNA\u6d4b\u5e8f\u6280\u672f\" title=\"ctDNA\u68c0\u6d4b\u7684\u79d8\u5bc6\u6b66\u5668\u2014Duplex UMI-ctDNA\u6d4b\u5e8f\u6280\u672f\"  \/><\/p>\n<blockquote class='keep-source'>\n<p>\u59cb\u53d1\u4e8e\u5fae\u4fe1\u516c\u4f17\u53f7\uff1a                         \u57fa\u56e0\u8c37                      <\/p>\n<\/blockquote>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>1 \u6db2\u4f53\u6d3b\u68c0\u5f25\u8865\u7ec4\u7ec7\u68c0\u6d4b\u7684\u4e0d\u8db3 \u540c\u4e00\u764c\u79cd\u7684\u4e0d\u540c\u60a3\u8005\uff0c\u540c\u4e00\u4e2a\u80bf\u7624\u60a3\u8005\u7684\u4e0d\u540c\u6cbb\u7597\u9636\u6bb5\u3001\u540c\u4e00\u80bf\u7624\u7ec4\u7ec7\u7684\u4e0d\u540c\u533a\u57df\uff0c\u80bf\u7624 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":30962,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[2],"tags":[],"_links":{"self":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/30961"}],"collection":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/comments?post=30961"}],"version-history":[{"count":1,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/30961\/revisions"}],"predecessor-version":[{"id":30973,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/30961\/revisions\/30973"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media\/30962"}],"wp:attachment":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media?parent=30961"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/categories?post=30961"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/tags?post=30961"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}