{"id":31389,"date":"2018-08-07T01:00:00","date_gmt":"2018-08-09T04:15:53","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/2018\/08\/09\/%e5%8d%87%e7%ba%a7crispr%ef%bc%9fcell%e5%ad%90%e5%88%8a%e6%8f%ad%e7%a4%ba%e6%af%94cas9%e6%9b%b4%e6%9c%89%e6%95%88%e3%80%81%e6%9b%b4%e5%ae%89%e5%85%a8%e7%9a%84%e9%85%b6\/"},"modified":"2018-08-09T12:15:55","modified_gmt":"2018-08-09T04:15:55","slug":"%e5%8d%87%e7%ba%a7crispr%ef%bc%9fcell%e5%ad%90%e5%88%8a%e6%8f%ad%e7%a4%ba%e6%af%94cas9%e6%9b%b4%e6%9c%89%e6%95%88%e3%80%81%e6%9b%b4%e5%ae%89%e5%85%a8%e7%9a%84%e9%85%b6","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/31389\/","title":{"rendered":"\u201c\u5347\u7ea7\u201dCRISPR\uff1fCell\u5b50\u520a\u63ed\u793a\u6bd4Cas9\u66f4\u6709\u6548\u3001\u66f4\u5b89\u5168\u7684\u9176"},"content":{"rendered":"<div class=\"bpp-post-content\">\n<section  class=\"\" style=\"font-size: 16px;white-space: normal;max-width: 100%;text-align: justify;background-color: rgb(255, 255, 255);letter-spacing: 0.476px;color: rgb(62, 62, 62);font-family: \u5fae\u8f6f\u96c5\u9ed1;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section class=\"\" style=\"max-width: 100%;border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section class=\"\" style=\"max-width: 100%;border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section class=\"\" style=\"padding: 0.8em;max-width: 100%;border-width: 1px 0.4em;border-style: solid;border-color: rgb(172, 29, 16);border-radius: 0.3em;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<p style=\"max-width: 100%;min-height: 1em;box-sizing: border-box !important;word-wrap: break-word !important;\">Cas9\u662fCRISPR\u57fa\u56e0\u7f16\u8f91\u6700\u6d41\u884c\u7684\u9176\uff0c\u4f46\u79d1\u5b66\u5bb6\u4eec\u6700\u8fd1\u627e\u5230\u4e86\u786e\u51ff\u7684\u8bc1\u636e\uff0c\u8bc1\u660e\u5176\u6548\u529b\u548c\u7cbe\u786e\u5ea6\u4f4e\u4e8e\u8f83\u5c11\u4f7f\u7528\u7684CRISPR\u86cb\u767dCas12a\u3002 \u7531\u4e8eCas9\u66f4\u6709\u53ef\u80fd\u9519\u8bef\u5730\u7f16\u8f91\u690d\u7269\u6216\u52a8\u7269\u7684\u57fa\u56e0\u7ec4\uff0c\u7834\u574f\u5176\u6b63\u5e38\u7684\u529f\u80fd\uff0c\u6240\u4ee5\u79d1\u5b66\u5bb6\u4eec\u5efa\u8bae\u4f7f\u7528Cas12a\u4ee5\u786e\u4fdd\u66f4\u5b89\u5168\u6709\u6548\u7684\u57fa\u56e0\u7f16\u8f91\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"text-align: justify;line-height: 1.75em;\"><br  \/><\/p>\n<p style=\"text-align: justify;line-height: 1.75em;\"><img class=\"\"     style=\"margin-top: 6px;margin-bottom: 6px;border-width: 0px;border-style: none;border-color: initial;vertical-align: middle;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/08\/beepress2-1533788154.png\" alt=\"\u201c\u5347\u7ea7\u201dCRISPR\uff1fCell\u5b50\u520a\u63ed\u793a\u6bd4Cas9\u66f4\u6709\u6548\u3001\u66f4\u5b89\u5168\u7684\u9176\" title=\"\u201c\u5347\u7ea7\u201dCRISPR\uff1fCell\u5b50\u520a\u63ed\u793a\u6bd4Cas9\u66f4\u6709\u6548\u3001\u66f4\u5b89\u5168\u7684\u9176\"  \/><br  \/><\/p>\n<p style=\"text-align: justify;line-height: 1.75em;\"><br  \/><\/p>\n<p style=\"text-align: justify;line-height: 1.75em;\">CRISPR\u662f\u8fd1\u5e74\u6765\u6700\u91cd\u8981\u7684\u79d1\u5b66\u7a81\u7834\u4e4b\u4e00\uff0c\u8fd9\u79cd\u6280\u672f\u53ef\u4ee5\u5feb\u901f\u4e14\u5ec9\u4ef7\u7684\u5bf9\u57fa\u56e0\u8fdb\u884c\u6539\u9020\u3002<\/p>\n<p><br  \/><\/p>\n<p style=\"text-align: justify;line-height: 1.75em;\">\u6700\u8fd1\uff0c\u5fb7\u514b\u8428\u65af\u5927\u5b66\u5965\u65af\u6c40\u5206\u6821\u7684\u79d1\u5b66\u5bb6\u4eec\u8868\u793a\uff0c\u4ed6\u4eec\u5df2\u7ecf\u5347\u7ea7\u4e86\u8fd9\u9879\u6280\u672f\uff0c\u4f7f\u5176\u5177\u5907\u66f4\u51c6\u786e\u7684\u57fa\u56e0\u7f16\u8f91\u80fd\u529b\u548c\u66f4\u9ad8\u7684\u5b89\u5168\u6027\u3002\u8fd9\u4e00\u7814\u7a76\u6709\u671b\u6253\u5f00\u5b9e\u7528\u6027\u57fa\u56e0\u7f16\u8f91\u7684\u5927\u95e8\uff0c\u8db3\u4ee5\u5b89\u5168\u5730\u7528\u4e8e\u4eba\u7c7b\u3002<\/p>\n<p><br  \/><\/p>\n<p style=\"text-align: justify;line-height: 1.75em;\">\u7814\u7a76\u56e2\u961f\u53d1\u73b0\uff0c<strong>CRISPR\u57fa\u56e0\u7f16\u8f91\u7cfb\u7edf\u4e2d\u7b2c\u4e00\u4e2a\u4e14\u5e94\u7528\u6700\u666e\u904d\u7684Cas9\u9176\uff0c\u5728\u6548\u529b\u548c\u7cbe\u786e\u5ea6\u4f4e\u4e8e\u53e6\u4e00\u79cd\u5e94\u7528\u8f83\u5c11\u7684Cas12a\u9176\u3002<\/strong>\u76f8\u5173\u7814\u7a76\u6210\u679c\u4e8e8\u67082\u65e5\u53d1\u8868\u5728Cell\u5b50\u520a\u300aMolecular cell\u300b\u4e0a\u3002<\/p>\n<p><br  \/><\/p>\n<p style=\"text-align: justify;line-height: 1.75em;\">\u201c\u603b\u4f53\u76ee\u6807\u662f\u627e\u5230\u5927\u81ea\u7136\u8d4b\u4e88\u6211\u4eec\u7684\u6700\u4f73\u9176\uff0c\u7136\u540e\u4f7fCRISPR\u57fa\u56e0\u7f16\u8f91\u7cfb\u7edf\u8fd0\u8f6c\u5f97\u66f4\u597d\uff0c\u6211\u4eec\u4e0d\u5e94\u8be5\u4ec5\u6ee1\u8db3\u4e8e\u4f7f\u7528\u7b2c\u4e00\u79cd\u53d1\u73b0\u7684\u9176\u3002\u201d\u672c\u7814\u7a76\u7684\u5408\u4f5c\u7814\u7a76\u8005Ilya Finkelstein\u6559\u6388\u8bf4\u3002<\/p>\n<p style=\"text-align: justify;line-height: 1.75em;\"><br  \/><\/p>\n<section class=\"\"  style=\"white-space: normal;max-width: 100%;color: rgb(51, 51, 51);\">\n<section style=\"margin: 10px auto;max-width: 100%;text-align: center;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section style=\"margin-bottom: 3px;margin-left: 33px;max-width: 100%;font-size: 0px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"margin-bottom: 10px;max-width: 100%;width: 6px;height: 6px;display: inline-block;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);\"><\/span><span style=\"max-width: 100%;display: inline-block;width: 10px;height: 10px;opacity: 0.6;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);\"><\/span>&nbsp;&nbsp;&nbsp;<\/span><\/section>\n<section style=\"margin-top: -2px;max-width: 100%;font-size: 0px;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;display: inline-block;width: 15px;height: 15px;border-width: 1px;border-style: solid;border-color: white;opacity: 0.9;vertical-align: bottom;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;display: inline-block;border-width: 1px;border-style: solid;border-color: rgb(255, 255, 255);width: 30px;height: 30px;line-height: 30px;vertical-align: top;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\">\n<p class=\"\" style=\"margin-right: auto;margin-left: auto;max-width: 100%;min-height: 1em;white-space: pre-wrap;min-width: 1px;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;\">01<\/p>\n<\/section>\n<section style=\"max-width: 100%;display: inline-block;width: 15px;height: 15px;border-width: 1px;border-style: solid;border-color: white;opacity: 0.9;vertical-align: top;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/section>\n<\/section>\n<section style=\"margin-top: 3px;margin-left: -33px;max-width: 100%;font-size: 0px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;display: inline-block;width: 10px;height: 10px;opacity: 0.6;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);\"><\/span><span style=\"margin-bottom: -10px;max-width: 100%;width: 6px;height: 6px;display: inline-block;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);\"><\/span>&nbsp; &nbsp;<\/span><\/section>\n<\/section>\n<\/section>\n<p style=\"white-space: normal;max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);text-align: center;\"><span style=\"max-width: 100%;font-size: 16px;color: rgb(123, 12, 0);box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;text-align: justify;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"text-align: justify;\">Cas9\u7684\u5c40\u9650\u6027<\/span><\/span><\/strong><\/span><\/p>\n<p style=\"white-space: normal;max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);text-align: center;\"><span style=\"max-width: 100%;font-size: 16px;color: rgb(123, 12, 0);box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;text-align: justify;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"text-align: justify;\"><br  \/><\/span><\/span><\/strong><\/span><\/p>\n<p style=\"text-align: justify;line-height: 1.75em;\">CRISPR\u57fa\u56e0\u7f16\u8f91\u6280\u672f\u662f\u7ec6\u83cc\u7528\u6765\u62b5\u5fa1\u75c5\u6bd2\u7684\u4e00\u79cd\u5929\u7136\u673a\u5236\uff0c\u901a\u8fc7\u5b83\uff0c\u79d1\u5b66\u5bb6\u80fd\u591f\u66f4\u591a\u5730\u4e86\u89e3\u4eba\u7c7b\u57fa\u56e0\uff0c\u5e76\u53ef\u4ee5\u5bf9\u690d\u7269\u3001\u52a8\u7269\u4e43\u81f3\u4eba\u4f53\u8fdb\u884c\u57fa\u56e0\u6539\u9020\u3002\u672a\u6765\uff0c\u8bb8\u591a\u4eba\u5e0c\u671bCRISPR\u80fd\u591f\u5e2e\u52a9\u6cbb\u7597\u4eba\u7c7b\u75be\u75c5\uff0c\u4ee5\u53ca\u521b\u9020\u51fa\u5177\u6709\u66f4\u9ad8\u4ea7\u91cf\u6216\u80fd\u62b5\u6297\u5e72\u65f1\u548c\u5bb3\u866b\u7684\u519c\u4f5c\u7269\u3002<\/p>\n<p><br  \/><\/p>\n<p style=\"text-align: justify;line-height: 1.75em;\">\u4f46\u662f<strong>\u76ee\u524d\u6d41\u884c\u7684CRISPR\/Cas9\u7cfb\u7edf\u6709\u65f6\u4f1a\u9776\u5411\u57fa\u56e0\u7ec4\u4e2d\u7684\u9519\u8bef\u4f4d\u7f6e<\/strong>\uff0c\u5c06\u8fd9\u79cd\u65b9\u6cd5\u76f4\u63a5\u5e94\u7528\u4e8e\u4eba\u7c7b\u6709\u65f6\u662f\u707e\u96be\u6027\u7684\uff0c\u5b83\u53ef\u80fd\u65e0\u6cd5\u6cbb\u6108\u9057\u4f20\u75be\u75c5\uff0c\u8fd8\u4f1a\u5c06\u5065\u5eb7\u7ec6\u80de\u8f6c\u53d8\u4e3a\u764c\u7ec6\u80de\u3002<\/p>\n<p style=\"text-align: justify;line-height: 1.75em;\"><br  \/><\/p>\n<p style=\"text-align: justify;line-height: 1.75em;\"><br  \/><\/p>\n<p style=\"white-space: normal;max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);text-align: center;\"><span style=\"text-align: justify;max-width: 100%;font-size: 16px;color: rgb(136, 136, 136);box-sizing: border-box !important;word-wrap: break-word !important;\">Cas12a\u4e0eDNA\u7ed3\u5408<\/span><\/p>\n<p style=\"white-space: normal;max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);text-align: center;\"><span style=\"max-width: 100%;font-size: 16px;color: rgb(123, 12, 0);box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;text-align: justify;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"text-align: justify;\"><br  \/><\/span><\/span><\/strong><\/span><\/p>\n<p style=\"text-align: justify;line-height: 1.75em;\">\u4e4b\u524d\u5df2\u6709\u7684\u4e00\u4e9b\u7814\u7a76\u6697\u793aCas12a\u6bd4Cas9\u66f4\u5177\u9009\u62e9\u6027\uff0c\u4f46\u4ecd\u6709\u8f83\u5927\u4e89\u8bae\u3002\u7814\u7a76\u4eba\u5458\u8ba4\u4e3a\uff0c\u4ed6\u4eec\u7684\u8fd9\u9879\u6700\u65b0\u7814\u7a76\u8bc1\u660e\u4e86Cas12a\u662f\u4e00\u79cd\u6bd4Cas9\u66f4\u7cbe\u786e\u7684\u57fa\u56e0\u7f16\u8f91\u624b\u672f\u5200\uff0c\u4e3a\u5b66\u672f\u754c\u7684\u4e89\u8bba\u753b\u4e0b\u4e86\u53e5\u53f7\u3002<\/p>\n<p><br  \/><\/p>\n<section class=\"\"  style=\"white-space: normal;max-width: 100%;color: rgb(51, 51, 51);\">\n<section style=\"margin: 10px auto;max-width: 100%;text-align: center;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section style=\"margin-bottom: 3px;margin-left: 33px;max-width: 100%;font-size: 0px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"margin-bottom: 10px;max-width: 100%;width: 6px;height: 6px;display: inline-block;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);\"><\/span><span style=\"max-width: 100%;display: inline-block;width: 10px;height: 10px;opacity: 0.6;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);\"><\/span>&nbsp;&nbsp;&nbsp;<\/span><\/section>\n<section style=\"margin-top: -2px;max-width: 100%;font-size: 0px;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;display: inline-block;width: 15px;height: 15px;border-width: 1px;border-style: solid;border-color: white;opacity: 0.9;vertical-align: bottom;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;display: inline-block;border-width: 1px;border-style: solid;border-color: rgb(255, 255, 255);width: 30px;height: 30px;line-height: 30px;vertical-align: top;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\">\n<p class=\"\" style=\"margin-right: auto;margin-left: auto;max-width: 100%;min-height: 1em;white-space: pre-wrap;min-width: 1px;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;\">02<\/p>\n<\/section>\n<section style=\"max-width: 100%;display: inline-block;width: 15px;height: 15px;border-width: 1px;border-style: solid;border-color: white;opacity: 0.9;vertical-align: top;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/section>\n<\/section>\n<section style=\"margin-top: 3px;margin-left: -33px;max-width: 100%;font-size: 0px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;display: inline-block;width: 10px;height: 10px;opacity: 0.6;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);\"><\/span><span style=\"margin-bottom: -10px;max-width: 100%;width: 6px;height: 6px;display: inline-block;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);\"><\/span>&nbsp; &nbsp;<\/span><\/section>\n<\/section>\n<\/section>\n<p style=\"white-space: normal;max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);text-align: center;\"><span style=\"max-width: 100%;font-size: 16px;color: rgb(123, 12, 0);box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;text-align: justify;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"text-align: justify;\">Cas12a\u7684\u4f18\u52bf<\/span><\/span><\/strong><\/span><\/p>\n<p style=\"white-space: normal;max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);text-align: center;\"><span style=\"max-width: 100%;font-size: 16px;color: rgb(123, 12, 0);box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;text-align: justify;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"text-align: justify;\"><br  \/><\/span><\/span><\/strong><\/span><\/p>\n<p style=\"text-align: justify;line-height: 1.75em;\">\u7531Isabel Strohkendl\u548cRick Russell\u6559\u6388\u9886\u5bfc\u7684\u7814\u7a76\u5c0f\u7ec4\u53d1\u73b0\uff0c<strong>Cas12a<\/strong>\u5e26\u6709\u4e00\u5c0f\u6bb5\u7528RNA\u7f16\u5199\u7684\u9057\u4f20\u5bc6\u7801\uff0c\u4f1a\u4e0e\u75c5\u6bd2DNA\u4e2d\u7f16\u5199\u7684\u76ee\u6807\u9057\u4f20\u5bc6\u7801\u4e32\u76f8\u5339\u914d\u3002\u5f53\u78b0\u5230\u4e00\u4e9bDNA\u65f6\uff0cCas12a\u5f00\u59cb\u8bd5\u56fe\u901a\u8fc7\u5f62\u6210\u78b1\u57fa\u5bf9\u6765\u4e0e\u5b83\u7ed3\u5408\uff0c\u5e76\u4ece\u4e00\u7aef\u5f00\u59cb\u524d\u8fdb\uff0c\u4ee5\u68c0\u6d4bDNA\u4e0eRNA\u76f8\u5339\u914d\u7684\u7a0b\u5ea6\u3002\u5b83\u4f1a\u50cf\u9b54\u672f\u8d34\u4e00\u6837\u7ed1\u5b9a\u5230\u57fa\u56e0\u7ec4\u76ee\u6807\u4e0a\uff0c\u5728\u6cbf\u9014\u7684\u6bcf\u4e2a\u70b9\u7d27\u5bc6\u7ed3\u5408\u5374\u53c8\u4e0d\u4f1a\u7262\u56fa\u5230\u96be\u4ee5\u5206\u79bb\uff0c\u8fd9\u6837\u5b83\u4f1a\u5728\u6574\u4e2a\u533a\u57df\u90fd\u6709\u826f\u597d\u5339\u914d\u65f6\u624d\u80fd\u987a\u5229\u8fdb\u884c\u7f16\u8f91\u3002<\/p>\n<p><br  \/><\/p>\n<p style=\"text-align: justify;line-height: 1.75em;\">\u800cCas9\u7684\u6bcf\u4e2a\u78b1\u57fa\u5bf9\u4f1a\u7d27\u7d27\u5730\u7c98\u5728\u4e00\u8d77\uff0c\u5c31\u50cf502\u80f6\u6c34\u4e00\u6837\u3002\u5982\u679c\u6bcf\u4fa7\u7684\u524d\u51e0\u4e2a\u5b57\u6bcd\u5339\u914d\u826f\u597d\uff0c\u90a3\u4e48Cas9\u5df2\u7ecf\u4e0eDNA\u7d27\u5bc6\u7ed3\u5408\u4e86\u3002\u6362\u53e5\u8bdd\u8bf4\uff0cCas9\u4f1a\u5173\u6ce8\u57fa\u56e0\u7ec4\u76ee\u6807\u4e2d\u7684\u524d\u4e03\u4e2a\u6216\u516b\u4e2a\u5b57\u6bcd\uff0c\u4f46\u540e\u9762\u7684\u8fc7\u7a0b\u5374\u4e0d\u592a\u4f1a\u88ab\u6ce8\u610f\uff0c\u8fd9\u610f\u5473\u7740\u5b83\u5f88\u5bb9\u6613\u5ffd\u7565\u8fc7\u7a0b\u4e2d\u7684\u4e0d\u5339\u914d\uff0c\u4ece\u800c\u5bfc\u81f4\u4e86\u5b83\u7684\u7f16\u8f91\u9519\u8bef\u3002<\/p>\n<p><br  \/><\/p>\n<p style=\"text-align: justify;line-height: 1.75em;\">\u201cCas12a\u7684\u7ed3\u5408\u65b9\u5f0f\u4f7f\u5f97\u78b1\u57fa\u5bf9\u5f62\u6210\u7684\u8fc7\u7a0b\u66f4\u52a0\u53ef\u9006\u3002\u201dIsabel\u89e3\u91ca\u9053\uff0c \u201c\u6362\u53e5\u8bdd\u8bf4\uff0cCas12a\u53ef\u4ee5\u66f4\u597d\u5730\u68c0\u67e5\u6bcf\u4e2a\u78b1\u57fa\u5bf9\uff0c\u7136\u540e\u518d\u8f6c\u5230\u4e0b\u4e00\u4e2a\u78b1\u57fa\u5bf9\u3002\u4e03\u5230\u516b\u4e2a\u5b57\u6bcd\u540e\uff0cCas9\u505c\u6b62\u68c0\u67e5\uff0c\u800cCas12a\u7ee7\u7eed\u68c0\u67e5\u5927\u7ea618\u4e2a\u5b57\u6bcd\u3002\u201d<\/p>\n<p><br  \/><\/p>\n<p style=\"text-align: justify;line-height: 1.75em;\">\u4f46\u7814\u7a76\u4eba\u5458\u8868\u793aCas12a\u4ecd\u7136\u4e0d\u591f\u5b8c\u7f8e\uff0c\u8be5\u7814\u7a76\u8fd8\u63d0\u51fa\u4e86\u8fdb\u4e00\u6b65\u6539\u8fdbCas12a\u7684\u8bbe\u60f3\uff0c\u4e5f\u8bb8\u6709\u4e00\u5929\u4f1a\u5b9e\u73b0\u521b\u5efa\u201c\u7cbe\u786e\u624b\u672f\u5200\u201d\u7684\u68a6\u60f3\uff0c\u5373\u4e00\u79cd\u57fa\u672c\u4e0a\u65e0\u9519\u7684\u57fa\u56e0\u7f16\u8f91\u5de5\u5177\u3002\u201c\u603b\u7684\u6765\u8bf4\uff0cCas12a\u66f4\u597d\uff0c\u4f46\u662f\u4f9d\u7136\u6709\u7f3a\u9677\u3002\u6240\u4ee5\uff0c\u6211\u4eec\u8fd8\u6709\u5f88\u591a\u6311\u6218\u3002\u201dFinkelstein\u8868\u793a\u9053\u3002<\/p>\n<p><br  \/><\/p>\n<p style=\"text-align: right;line-height: 1.75em;\"><strong>\u8d23\u7f16\uff1a\u6d6e\u82cf<\/strong><\/p>\n<section  class=\"\" style=\"white-space: normal;max-width: 100%;color: rgb(51, 51, 51);\">\n<section  style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section class=\"\" style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section  class=\"\" style=\"max-width: 100%;line-height: 28.4444px;font-family: \u5fae\u8f6f\u96c5\u9ed1;border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box !important;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<section  style=\"margin: 1em auto;max-width: 100%;border-width: 0px;border-style: none;border-color: initial;text-align: center;width: 941px;box-sizing: border-box !important;word-wrap: break-word !important;overflow-wrap: break-word !important;\"><span class=\"\"  style=\"max-width: 100%;width: 941px;border-bottom: 1px solid rgb(172, 29, 16);border-top-color: rgb(172, 29, 16);border-right-color: rgb(172, 29, 16);border-left-color: rgb(172, 29, 16);display: inline-block;box-sizing: border-box !important;word-wrap: break-word !important;overflow-wrap: break-word !important;\"><\/p>\n<section class=\"\" style=\"margin-bottom: -3px;padding-top: 5px;padding-right: 10px;padding-left: 10px;max-width: 100%;display: inline-block;min-height: 32px;border-bottom: 5px solid rgb(172, 29, 16);font-size: 20px;color: rgb(121, 121, 121);box-sizing: border-box !important;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<p class=\"\" style=\"margin-top: 5px;margin-bottom: 5px;max-width: 100%;min-height: 1em;line-height: 1.6em;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;overflow-wrap: break-word !important;\">End<\/p>\n<\/section>\n<p><\/span><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p class=\"\" style=\"white-space: normal;max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 12px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u53c2\u8003\u8d44\u6599\uff1a1\uff09<\/span><span style=\"font-size: 12px;\"><span style=\"text-align: justify;\">How to make the gene-editing tool CRISPR work even better<\/span><\/span><\/p>\n<p style=\"white-space: normal;text-align: justify;\"><br  \/><\/p>\n<section  class=\"\" style=\"font-size: 16px;white-space: normal;max-width: 100%;box-sizing: border-box;letter-spacing: 0.544px;text-align: justify;background-color: rgb(255, 255, 255);color: rgb(62, 62, 62);line-height: 25.6px;font-family: \u5fae\u8f6f\u96c5\u9ed1;border-width: 0px;border-style: none;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<section class=\"\"  style=\"padding: 2px;max-width: 100%;box-sizing: border-box;border-width: 1px;border-style: dotted;border-color: rgb(218, 98, 71);word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<section class=\"\" style=\"margin: 5px auto;max-width: 100%;box-sizing: border-box;border-width: 0px;border-style: none;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<section class=\"\" style=\"max-width: 100%;box-sizing: border-box;height: 25px;color: inherit;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<section class=\"\" style=\"max-width: 100%;box-sizing: border-box;height: 25px;width: 50px;float: left;border-top: 2px solid rgb(172, 29, 16);border-right-color: rgb(172, 29, 16);border-bottom-color: rgb(172, 29, 16);border-left: 2px solid rgb(172, 29, 16);color: inherit;word-wrap: break-word !important;overflow-wrap: break-word !important;\"><\/section>\n<section class=\"\" style=\"max-width: 100%;box-sizing: border-box;display: inline-block;color: rgb(172, 29, 16);clear: both;border-color: rgb(172, 29, 16);word-wrap: break-word !important;overflow-wrap: break-word !important;\"><\/section>\n<\/section>\n<section class=\"\"  style=\"margin: -0.8em 0.3em;padding: 0.8em;max-width: 100%;box-sizing: border-box;color: inherit;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<p class=\"\" style=\"max-width: 100%;min-height: 1em;overflow-wrap: break-word;color: rgb(51, 51, 51);font-size: 1em;line-height: 1.75em;word-break: break-all;box-sizing: border-box !important;word-wrap: break-word !important;\"><span class=\"\" style=\"max-width: 100%;color: rgb(0, 0, 0);line-height: 22.4px;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\u672c\u6587\u7cfb\u751f\u7269\u63a2\u7d22\u539f\u521b\uff0c\u6b22\u8fce\u4e2a\u4eba\u8f6c\u53d1\u5206\u4eab\u3002\u5176\u4ed6\u4efb\u4f55\u5a92\u4f53\u3001\u7f51\u7ad9\u5982\u9700\u8f6c\u8f7d\uff0c\u987b\u5728\u6b63\u6587\u524d\u6ce8\u660e\u6765\u6e90\u751f\u7269\u63a2\u7d22\u3002<\/span><\/p>\n<\/section>\n<section class=\"\" style=\"max-width: 100%;box-sizing: border-box;height: 25px;color: inherit;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<section class=\"\" style=\"max-width: 100%;box-sizing: border-box;height: 25px;width: 50px;float: right;border-bottom: 2px solid rgb(172, 29, 16);border-top-color: rgb(172, 29, 16);border-right: 2px solid rgb(172, 29, 16);border-left-color: rgb(172, 29, 16);color: inherit;word-wrap: break-word !important;overflow-wrap: break-word !important;\"><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p class=\"\" style=\"white-space: normal;max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><img class=\"\"      width=\"auto\"  style=\"box-sizing: border-box !important;word-wrap: break-word !important;visibility: visible !important;width: auto !important;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/08\/beepress0-1533788154.png\" alt=\"\u201c\u5347\u7ea7\u201dCRISPR\uff1fCell\u5b50\u520a\u63ed\u793a\u6bd4Cas9\u66f4\u6709\u6548\u3001\u66f4\u5b89\u5168\u7684\u9176\" title=\"\u201c\u5347\u7ea7\u201dCRISPR\uff1fCell\u5b50\u520a\u63ed\u793a\u6bd4Cas9\u66f4\u6709\u6548\u3001\u66f4\u5b89\u5168\u7684\u9176\"  \/><\/p>\n<blockquote class='keep-source'>\n<p>\u59cb\u53d1\u4e8e\u5fae\u4fe1\u516c\u4f17\u53f7\uff1a                         \u751f\u7269\u63a2\u7d22                      <\/p>\n<\/blockquote>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Cas9\u662fCRISPR\u57fa\u56e0\u7f16\u8f91\u6700\u6d41\u884c\u7684\u9176\uff0c\u4f46\u79d1\u5b66\u5bb6\u4eec\u6700\u8fd1\u627e\u5230\u4e86\u786e\u51ff\u7684\u8bc1\u636e\uff0c\u8bc1\u660e\u5176\u6548\u529b\u548c\u7cbe\u786e\u5ea6\u4f4e\u4e8e\u8f83\u5c11\u4f7f\u7528\u7684CR [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":31390,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[2],"tags":[],"_links":{"self":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/31389"}],"collection":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/comments?post=31389"}],"version-history":[{"count":1,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/31389\/revisions"}],"predecessor-version":[{"id":31398,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/31389\/revisions\/31398"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media\/31390"}],"wp:attachment":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media?parent=31389"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/categories?post=31389"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/tags?post=31389"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}