{"id":31399,"date":"2018-08-06T01:00:00","date_gmt":"2018-08-09T04:15:57","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/2018\/08\/09\/%e4%b8%93%e8%ae%bf-%e5%a4%8d%e6%97%a6%e5%a4%a7%e5%ad%a6%e5%8d%a2%e5%a4%a7%e5%84%92%e6%95%99%e6%8e%88%ef%bc%9a%e5%9f%ba%e5%9b%a0%e7%bc%96%e8%be%91%e6%ad%a3%e5%bd%93%e6%97%b6%ef%bc%8c%e5%90%84\/"},"modified":"2018-08-09T12:15:59","modified_gmt":"2018-08-09T04:15:59","slug":"%e4%b8%93%e8%ae%bf-%e5%a4%8d%e6%97%a6%e5%a4%a7%e5%ad%a6%e5%8d%a2%e5%a4%a7%e5%84%92%e6%95%99%e6%8e%88%ef%bc%9a%e5%9f%ba%e5%9b%a0%e7%bc%96%e8%be%91%e6%ad%a3%e5%bd%93%e6%97%b6%ef%bc%8c%e5%90%84","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/31399\/","title":{"rendered":"\u4e13\u8bbf | \u590d\u65e6\u5927\u5b66\u5362\u5927\u5112\u6559\u6388\uff1a\u57fa\u56e0\u7f16\u8f91\u6b63\u5f53\u65f6\uff0c\u5404\u8272\u5e94\u7528\u663e\u8eab\u624b"},"content":{"rendered":"<div class=\"bpp-post-content\">\n<section class=\"\" label=\"Copyright \u00a9 2015 Yead All Rights Reserved.\" style=\"white-space: normal;max-width: 100%;color: rgb(51, 51, 51);\">\n<fieldset style=\"margin-top: 0.5em;margin-bottom: 0.5em;max-width: 100%;box-sizing: border-box;min-width: 0px;border-width: 0px;border-style: initial;border-color: initial;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<section class=\"\" style=\"margin-left: 5.51563px;max-width: 100%;box-sizing: border-box;border-width: 1px;border-style: solid;border-color: rgb(197, 63, 70);border-radius: 0px 5px 5px 0px;font-size: 1em;font-family: inherit;text-align: inherit;text-decoration: inherit;color: rgb(52, 54, 60);word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<section class=\"\" style=\"margin-top: 27.2656px;margin-right: 8px;margin-left: -8px;max-width: 100%;box-sizing: border-box;float: left;font-size: 1em;font-family: inherit;font-style: inherit;text-decoration: inherit;color: rgb(255, 255, 255);border-color: rgb(197, 63, 70);word-wrap: break-word !important;overflow-wrap: break-word !important;\"><span class=\"\" style=\"padding: 0.3em 0.5em;max-width: 100%;box-sizing: border-box;display: inline-block;border-radius: 0px 0.5em 0.5em 0px;font-size: 1em;font-family: inherit;background-color: rgb(197, 63, 70);word-wrap: break-word !important;overflow-wrap: break-word !important;\"><\/p>\n<section style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\u751f\u7269\u63a2\u7d22<\/section>\n<p><\/span><\/p>\n<section class=\"\" style=\"max-width: 100%;box-sizing: border-box;width: 0px;border-right: 4px solid rgb(197, 63, 70);border-top: 4px solid rgb(197, 63, 70);word-wrap: break-word !important;overflow-wrap: break-word !important;border-left: 4px solid transparent !important;border-bottom: 4px solid transparent !important;\"><\/section>\n<\/section>\n<section style=\"margin-top: 27.2656px;padding-right: 8px;padding-left: 8px;max-width: 100%;box-sizing: border-box;font-size: 1.5em;font-family: inherit;text-align: inherit;text-decoration: inherit;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;overflow-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 18px;box-sizing: border-box !important;word-wrap: break-word !important;overflow-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\u7f16\u8005\u6309<\/strong><\/span><\/section>\n<\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;clear: both;word-wrap: break-word !important;overflow-wrap: break-word !important;\"><\/section>\n<section style=\"padding: 8px;max-width: 100%;box-sizing: border-box;line-height: 30px;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<p style=\"max-width: 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style=\"white-space: normal;\">\n<section class=\"\"  style=\"white-space: normal;max-width: 100%;color: rgb(51, 51, 51);\">\n<section style=\"margin: 10px auto;max-width: 100%;text-align: center;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section style=\"margin-bottom: 3px;margin-left: 33px;max-width: 100%;font-size: 0px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"margin-bottom: 10px;max-width: 100%;width: 6px;height: 6px;display: inline-block;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><span style=\"max-width: 100%;display: inline-block;width: 10px;height: 10px;opacity: 0.6;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span>&nbsp;&nbsp;&nbsp;<\/span><\/section>\n<section style=\"margin-top: -2px;max-width: 100%;font-size: 0px;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;display: inline-block;width: 15px;height: 15px;border-width: 1px;border-style: solid;border-color: white;opacity: 0.9;vertical-align: bottom;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;display: inline-block;border-width: 1px;border-style: solid;border-color: rgb(255, 255, 255);width: 30px;height: 30px;line-height: 30px;vertical-align: top;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\">\n<p class=\"\" style=\"margin-right: auto;margin-left: auto;max-width: 100%;min-height: 1em;white-space: pre-wrap;min-width: 1px;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;\">01<\/p>\n<\/section>\n<section style=\"max-width: 100%;display: inline-block;width: 15px;height: 15px;border-width: 1px;border-style: solid;border-color: white;opacity: 0.9;vertical-align: top;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/section>\n<\/section>\n<section style=\"margin-top: 3px;margin-left: -33px;max-width: 100%;font-size: 0px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;display: inline-block;width: 10px;height: 10px;opacity: 0.6;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><span style=\"margin-bottom: -10px;max-width: 100%;width: 6px;height: 6px;display: inline-block;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span>&nbsp; &nbsp;<\/span><\/section>\n<\/section>\n<\/section>\n<p style=\"white-space: normal;max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);text-align: center;\"><span style=\"max-width: 100%;font-size: 16px;color: rgb(123, 12, 0);box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;text-align: justify;box-sizing: border-box !important;word-wrap: break-word !important;\">\u9057\u4f20\u5b66\u7684\u9b45\u529b<\/span><\/strong><\/span><\/p>\n<p style=\"white-space: normal;max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><\/p>\n<p style=\"white-space: normal;text-align: justify;line-height: 1.75em;\">20\u4e16\u7eaa70\u5e74\u4ee3\uff0c\u968f\u7740\u57fa\u56e0\u5de5\u7a0b\u7684\u8bde\u751f\uff0c\u9057\u4f20\u5b66\u6b63\u503c\u201c\u9633\u6625\u767d\u96ea\u201d\u30021984\u5e74\uff0c\u5362\u5927\u5112\u8003\u5165\u5fc3\u4eea\u5df2\u4e45\u7684\u590d\u65e6\u5927\u5b66\u751f\u7269\u5de5\u7a0b\u7cfb\uff0c\u5e08\u4ece\u6211\u56fd\u9057\u4f20\u5b66\u4e4b\u7236\u8c08\u5bb6\u6862\u5148\u751f\u3002\u4e0e\u4f20\u7edf\u7684\u751f\u7269\u5b66\u4e0d\u540c\uff0c\u9057\u4f20\u5b66\u5f3a\u8c03\u903b\u8f91\u63a8\u7406\u7684\u8fc7\u7a0b\u3002\u5728\u57fa\u56e0\u5de5\u7a0b\u9886\u57df\uff0c\u9057\u4f20\u5b66\u751a\u81f3\u53ef\u4ee5\u89e3\u7801\u57fa\u56e0\u3001\u8bbe\u8ba1\u57fa\u56e0\u3001\u64cd\u4f5c\u57fa\u56e0\u3001\u6539\u9020\u57fa\u56e0\uff0c\u8fd9\u4e00\u5207\u90fd\u8ba9\u5362\u5927\u5112\u611f\u53d7\u5230\u8fd9\u4e00\u5b66\u79d1\u7684\u9b45\u529b\u3002<\/p>\n<p style=\"white-space: normal;text-align: justify;line-height: 1.75em;\">&nbsp;<\/p>\n<p style=\"white-space: normal;text-align: justify;line-height: 1.75em;\">\u201c\u7814\u7a76\u751f\u7684\u65f6\u5019\uff0c\u6070\u9022\u8c08\u5bb6\u6862\u5148\u751f\u5411\u56fd\u5bb6\u5021\u8bae\uff0c\u63d0\u51fa\u4e86\u57fa\u56e0\u6cbb\u7597\u9057\u4f20\u75c5\u7684\u8bfe\u9898\uff0c\u6211\u5f88\u6709\u5e78\u53c2\u4e0e\u5230\u8fd9\u9879\u7814\u7a76\u4e2d\u3002\u7814\u7a76\u751f\u8bfb\u5b8c\u4ee5\u540e\uff0c\u6211\u6709\u51e0\u79cd\u9009\u62e9\uff0c\u4e00\u662f\u56de\u82cf\u5dde\u533b\u5b66\u7b2c\u4e00\u9644\u5c5e\u533b\u9662\uff08\u73b0\u5728\u7684\u82cf\u5dde\u5927\u5b66\u7b2c\u4e00\u9644\u5c5e\u533b\u9662\uff09\uff0c\u4e8c\u662f\u51fa\u56fd\uff0c\u4e09\u662f\u7ee7\u7eed\u653b\u8bfb\u535a\u58eb\u3002\u5f53\u65f6\u5e78\u5f97\u8c08\u5bb6\u6862\u5148\u751f\u548c\u5bfc\u5e08\u7684\u5668\u91cd\uff0c\u6211\u6700\u540e\u9009\u62e9\u7ee7\u7eed\u56de\u5230\u590d\u65e6\u5927\u5b66\u653b\u8bfb\u535a\u58eb\u3002\u201d\u91c7\u8bbf\u4e2d\uff0c\u5362\u6559\u6388\u56de\u5fc6\u4e86\u7ee7\u7eed\u5728\u590d\u65e6\u653b\u8bfb\u535a\u58eb\u7684\u6545\u4e8b\u3002<\/p>\n<p style=\"white-space: normal;text-align: justify;line-height: 1.75em;\">&nbsp;<\/p>\n<p style=\"white-space: normal;text-align: justify;line-height: 1.75em;\">\u5982\u4eca\uff0c\u7ecf\u8fc730\u591a\u5e74\u7684\u53d1\u5c55\uff0c\u9057\u4f20\u5b66\u79d1\u5b66\u548c\u6280\u672f\u5b9e\u5b9e\u5728\u5728\u5730\u5e94\u7528\u5728\u6211\u4eec\u65e5\u5e38\u751f\u6d3b\u7684\u65b9\u65b9\u9762\u9762\uff0c\u5305\u62ec\u4eba\u7c7b\u5065\u5eb7\u3001\u75be\u75c5\u8bca\u7597\u3001\u836f\u54c1\u3001\u519c\u4e1a\u3001\u73af\u5883\u4fdd\u62a4\u3001\u53f8\u6cd5\u7b49\u65b9\u9762\u3002\u5728\u5362\u5927\u5112\u770b\u6765\uff0c\u771f\u6b63\u63a8\u52a8\u9057\u4f20\u5b66\u4ece\u57fa\u7840\u79d1\u5b66\u5230\u5e94\u7528\u8f6c\u5316\u7684\u7b2c\u4e00\u4e2a\u5173\u952e\u6280\u672f\u662f\u91cd\u7ec4DNA\u6280\u672f\uff0c\u5b83\u5f15\u9886\u4e86\u79d1\u5b66\u9769\u547d\uff0c\u968f\u540e\u624d\u6709\u4e86\u57fa\u56e0\u5de5\u7a0b\u3001\u836f\u7269\u548c\u75ab\u82d7\u7b49\u3002\u4e0e\u6b64\u540c\u65f6\uff0cPCR\u6280\u672f\u7684\u51fa\u73b0\u63a8\u52a8\u4e86\u57fa\u56e0\u7814\u7a76\u7684\u53d1\u5c55\u3002\u201c\u5b83\u5c31\u50cf\u8ba9\u6211\u4eec\u5750\u4e0a\u4e86\u2018\u9ad8\u94c1\u2019\uff0c\u5f97\u4ee5\u7834\u89e3\u4eba\u7c7b\u751f\u547d\u7684\u5bc6\u7801\u3002\u201d\u5362\u6559\u6388\u89e3\u91ca\u9053\u3002<\/p>\n<section class=\"\"  style=\"white-space: normal;max-width: 100%;color: rgb(51, 51, 51);\">\n<section style=\"margin: 10px auto;max-width: 100%;text-align: center;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section style=\"margin-bottom: 3px;margin-left: 33px;max-width: 100%;font-size: 0px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"margin-bottom: 10px;max-width: 100%;width: 6px;height: 6px;display: inline-block;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><span style=\"max-width: 100%;display: inline-block;width: 10px;height: 10px;opacity: 0.6;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span>&nbsp;&nbsp;&nbsp;<\/span><\/section>\n<section style=\"margin-top: -2px;max-width: 100%;font-size: 0px;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;display: inline-block;width: 15px;height: 15px;border-width: 1px;border-style: solid;border-color: white;opacity: 0.9;vertical-align: bottom;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;display: inline-block;border-width: 1px;border-style: solid;border-color: rgb(255, 255, 255);width: 30px;height: 30px;line-height: 30px;vertical-align: top;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\">\n<p class=\"\" style=\"margin-right: auto;margin-left: auto;max-width: 100%;min-height: 1em;white-space: pre-wrap;min-width: 1px;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;\">02<\/p>\n<\/section>\n<section style=\"max-width: 100%;display: inline-block;width: 15px;height: 15px;border-width: 1px;border-style: solid;border-color: white;opacity: 0.9;vertical-align: top;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/section>\n<\/section>\n<section style=\"margin-top: 3px;margin-left: -33px;max-width: 100%;font-size: 0px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;display: inline-block;width: 10px;height: 10px;opacity: 0.6;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><span style=\"margin-bottom: -10px;max-width: 100%;width: 6px;height: 6px;display: inline-block;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span>&nbsp; &nbsp;<\/span><\/section>\n<\/section>\n<\/section>\n<p style=\"white-space: normal;max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);text-align: center;\"><span style=\"max-width: 100%;font-size: 16px;color: rgb(123, 12, 0);box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;text-align: justify;box-sizing: border-box !important;word-wrap: break-word !important;\">\u57fa\u56e0\u7f16\u8f91\u7684\u81ea\u7531\u63a2\u7d22<\/span><\/strong><\/span><\/p>\n<p style=\"white-space: normal;max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><\/p>\n<p style=\"white-space: normal;text-align: justify;line-height: 1.75em;\">\u5982\u4f55\u9605\u8bfb\u3001\u4fee\u6539\u751a\u81f3\u7f16\u5199\u4eba\u7c7b\u57fa\u56e0\u7ec4\u8fd9\u672c\u201c\u5929\u4e66\u201d\u5462\uff1f\u5362\u5927\u5112\u6559\u6388\u4e00\u8bed\u4e2d\u7684\uff0c\u7b54\u6848\u662f\u57fa\u56e0\u7f16\u8f91\u2014\u2014\u901a\u8fc7\u6539\u5199\u3001\u4fee\u9970\u5929\u4e66\uff0c\u8ba9\u5176\u5f97\u4ee5\u6b63\u5e38\u3001\u751a\u81f3\u4e8e\u5b8c\u7f8e\uff0c\u4fc3\u4f7f\u75be\u75c5\u7279\u522b\u662f\u9057\u4f20\u75c5\u80fd\u591f\u4ece\u6839\u672c\u4e0a\u5f97\u5230\u6cbb\u7597\u3002<\/p>\n<p style=\"white-space: normal;text-align: justify;line-height: 1.75em;\">&nbsp;<\/p>\n<p style=\"white-space: normal;text-align: justify;line-height: 1.75em;\">\u8c08\u5230\u57fa\u56e0\u7f16\u8f91\u7684\u524d\u4e16\uff0c\u4e00\u5b9a\u8981\u4ece\u540c\u6e90\u91cd\u7ec4\u8fdb\u884c\u57fa\u56e0\u6253\u9776\u8bf4\u8d77\u3002\u4e0a\u4e2a\u4e16\u7eaa80\u5e74\u4ee3\uff0c\u79d1\u5b66\u5bb6\u5728\u5c0f\u9f20\u80da\u80ce\u5e72\u7ec6\u80de\u4e2d\u901a\u8fc7\u57fa\u56e0\u6253\u9776\u6280\u672f\u5b9e\u73b0\u4e86\u57fa\u56e0\u7f16\u8f91\uff0c\u4f46\u6b64\u6280\u672f\u5728\u5176\u4f59\u7ec6\u80de\u5185\u6548\u7387\u6781\u4f4e\uff0c\u5e94\u7528\u53d7\u5230\u6781\u5927\u7684\u9650\u5236\u300290\u5e74\u4ee3\uff0c\u57fa\u4e8e\u7ec6\u80de\u5185\u4e0d\u540c\u950c\u6307\u86cb\u767d\u53ef\u7279\u5f02\u6027\u8bc6\u522bDNA\u4e0a\u4e09\u8054\u78b1\u57fa\u7684\u7279\u5f81\u4ee5\u53ca\u6838\u9178\u9176FokI\u4e8c\u805a\u5316\u540e\u53ef\u4ee5\u5207\u5272DNA\u7684\u7279\u70b9\uff0c\u79d1\u5b66\u5bb6\u4eec\u7814\u53d1\u51fa\u4e00\u79cd\u65b0\u7684\u57fa\u56e0\u7f16\u8f91\u6280\u672f\u2014\u2014\u950c\u6307\u86cb\u767d\u9178\u9176\u6280\u672f\uff08ZFNs\uff09\u3002\u4f46\u6b64\u6280\u672f\u4e13\u5229\u88ab\u516c\u53f8\u5784\u65ad\uff0c\u4e14\u950c\u6307\u86cb\u767d\u6570\u91cf\u6709\u9650\uff0c\u53ef\u4ee5\u8bc6\u522b\u7684DNA\u5e8f\u5217\u6570\u91cf\u6709\u9650\u3002<\/p>\n<p style=\"white-space: normal;text-align: justify;line-height: 1.75em;\">&nbsp;<\/p>\n<p style=\"white-space: normal;text-align: justify;line-height: 1.75em;\">2011\u5e74\u5de6\u53f3\uff0c TALENs\u6280\u672f\u6a2a\u7a7a\u51fa\u4e16\uff0c\u5b83\u662f\u4e00\u79cd\u4eba\u5de5\u6539\u9020\u7684\u9650\u5236\u6027\u5185\u5207\u9176\uff0c\u662f\u5c06TALE\u7684DNA\u7ed3\u5408\u57df\u57df\u9650\u5236\u6027\u5185\u5207\u9176\uff08FokI\uff09\u7684DNA\u5207\u5272\u57df\u878d\u5408\u800c\u5f97\u5230\u3002\u7406\u8bba\u4e0a\uff0cTALENs\u53ef\u4ee5\u4efb\u610f\u9009\u62e9\u9776DNA\u5e8f\u5217\u8fdb\u884c\u6539\u9020\uff0c\u662f\u4e00\u79cd\u975e\u5e38\u6709\u6548\u7684\u57fa\u56e0\u7ec4\u6539\u9020\u5de5\u5177\u9176\u3002<\/p>\n<p style=\"white-space: normal;text-align: justify;line-height: 1.75em;\">&nbsp;<\/p>\n<p style=\"white-space: normal;text-align: justify;line-height: 1.75em;\">2013\u5e74\u5f00\u59cb\uff0c\u57fa\u4e8e\u7ec6\u83cc\u89c4\u5f8b\u6210\u7c07\u7684\u95f4\u9694\u77ed\u56de\u6587\u91cd\u590d\u5e8f\u5217\uff08CRISPR\uff09\u7cfb\u7edf\u53d1\u5c55\u800c\u6765\u7684\u65b0\u4e00\u4ee3\u57fa\u56e0\u7ec4\u7f16\u8f91\u6280\u672f\u2014\u2014CRISPR\/Cas9\u6280\u672f\uff0c\u4f7f\u5f97\u57fa\u56e0\u7f16\u8f91\u53d8\u5f97\u66f4\u4e3a\u7b80\u6613\u3001\u9ad8\u6548\uff0c\u76ee\u524d\u5df2\u7ecf\u5e7f\u6cdb\u5e94\u7528\u4e8e\u591a\u7269\u79cd\u9776\u5411\u57fa\u56e0\u7f16\u8f91\u3001\u8f6c\u5f55\u8c03\u63a7\u3001\u8868\u89c2\u4fee\u9970\uff0c\u57fa\u56e0\u6cbb\u7597\u7814\u7a76\u548c\u4e34\u5e8a\u7814\u7a76\u3002<\/p>\n<p style=\"white-space: normal;text-align: justify;line-height: 1.75em;\">&nbsp;<\/p>\n<p style=\"white-space: normal;text-align: justify;line-height: 1.75em;\">\u5176\u5b9e\u65e9\u5728\u672c\u4e16\u7eaa\u521d\uff0c \u5362\u5927\u5112\u6559\u6388\u5c31\u5f00\u59cb\u5173\u6ce8\u8fd9\u4e9b\u6280\u672f\u3001\u5e76\u5408\u4f5c\u5f00\u5c55\u4e86ZFN\u3001\u72ec\u7acb\u8fdb\u884c\u4e86TALEN\u7684\u57fa\u56e0\u7f16\u8f91\u7814\u7a76\u4e86\uff0c2012\u5e74\uff0c\u5362\u5927\u5112\u6559\u6388\u7684\u56e2\u961f\u5c31\u5df2\u7ecf\u770b\u5230CRISPR\u6280\u672f\u7684\u6f5c\u529b\uff0c\u5e76\u4e8e2013\u5e74\u6625\u5929\uff0c\u5c06\u7814\u7a76\u56e2\u961f\u7684\u7cbe\u529b\u4eceTALENs\u8f6c\u5230CRISPR\u4e0a\uff0c\u6784\u5efa\u4e00\u4e2a\u8840\u53cb\u75c5B\u5c0f\u9f20\u6a21\u578b\uff0c\u5e76\u8fdb\u884c\u4e86\u8840\u53cb\u75c5B\u5c0f\u9f20\u7684\u57fa\u56e0\u7f16\u8f91\u6cbb\u7597\u7814\u7a76\u3002\u800c\u73b0\u5728\u4ed6\u4eec\u56e2\u961f\u7684\u7814\u7a76\u91cd\u70b9\u4e4b\u4e00\u662f\u5730\u4e2d\u6d77\u8d2b\u8840\u75c7\u57fa\u56e0\u7f16\u8f91\u6cbb\u7597\u3002\u5177\u4f53\u6765\u8bf4\uff0c\u91c7\u7528CRISPR\u7684RNP\u901a\u8fc7\u86cb\u767d\u8d28\u8f6c\u5bfc\u7684\u65b9\u6cd5\uff0c\u5efa\u7acb\u4e86\u6210\u719f\u7684\u9020\u8840\u5e72\u7ec6\u80de\u5206\u79bb\u3001\u57f9\u517b\u548c\u6269\u589e\u6280\u672f\uff0c\u9020\u8840\u5e72\u7ec6\u80de\u7684\u7ec6\u80de\u5206\u5316\u6280\u672f\uff0c\u591a\u79cd\u9020\u8840\u5e72\u7ec6\u80de\u7684\u57fa\u56e0\u548cRNP\u8f6c\u67d3\u7cfb\u7edf\uff08\u5305\u62ec\u6162\u75c5\u6bd2\u3001\u817a\u76f8\u5173\u75c5\u6bd2\u548c\u7535\u8f6c\u67d3\u7b49\uff09\uff0c\u4ed6\u4eec\u57fa\u4e8eNSG\u5c0f\u9f20\u7684\u4eba\u9020\u8840\u5e72\u7ec6\u80de\u79fb\u690d\u6a21\u578b\u89c2\u5bdf\u6cbb\u7597\u6548\u679c\uff0c\u76ee\u524d\u5df2\u53d6\u5f97\u521d\u6b65\u8fdb\u5c55\u548c\u6210\u679c\u3002<\/p>\n<p style=\"white-space: normal;text-align: justify;line-height: 1.75em;\">&nbsp;<\/p>\n<p style=\"white-space: normal;text-align: justify;line-height: 1.75em;\">\u5362\u5927\u5112\u6559\u6388\u4e5f\u63d0\u5230\uff0c\u201cCRISPR\/Cas9\u4e5f\u6709\u5f88\u591a\u9700\u8981\u4e0d\u65ad\u5b8c\u5584\u548c\u53d1\u5c55\u7684\u5730\u65b9\uff0c\u6bd4\u5982CRISPR\/Cas9\u53ef\u80fd\u4f1a\u6709\u8131\u9776\u6548\u5e94\uff0c\u8fd9\u4e9b\u90fd\u8ba9\u6211\u4eec\u610f\u8bc6\u5230\u4efb\u4f55\u6280\u672f\u7684\u53d1\u5c55\u90fd\u9700\u8981\u87ba\u65cb\u5f0f\u4e0a\u5347\uff0c\u5728\u771f\u6b63\u4e34\u5e8a\u5e94\u7528\u4e4b\u524d\u9700\u8981\u4e0d\u65ad\u6539\u8fdb\uff0c\u4ee5\u4fbf\u8ba9\u5176\u66f4\u52a0\u7cbe\u51c6\u3002\u76ee\u524d\u6211\u4eec\u56e2\u961f\u91c7\u7528\u4f4e\u6e29\u51b7\u51bb\u7535\u955c\u7b49\u6280\u672f\u7814\u7a76CRISPR\/Cas9\uff0c\u76ee\u7684\u4e5f\u662f\u901a\u8fc7\u7a81\u53d8\u6539\u9020\uff0c\u63d0\u9ad8\u7cbe\u51c6\u5ea6\u3002\u6b64\u5916\uff0c\u6211\u4eec\u6b63\u5728\u601d\u8003\u662f\u5426\u80fd\u591f\u63d0\u9ad8CRISPR\/Cas9\u4e00\u4e9b\u4f4e\u6548\u7684\u533a\u57df\u5207\u5272\u6548\u7387\u7b49\u95ee\u9898\u3002\u5728\u6cbb\u7597\u9057\u4f20\u75c5\u7684\u5c1d\u8bd5\u4e2d\uff0c\u57fa\u56e0\u7f16\u8f91\u548c\u5355\u78b1\u57fa\u7f16\u8f91\u8fd9\u4e24\u79cd\u6280\u672f\u53ef\u4ee5\u4e92\u8865\u3002\u201d<\/p>\n<p style=\"white-space: normal;text-align: justify;line-height: 1.75em;\">&nbsp;<\/p>\n<p style=\"white-space: normal;text-align: justify;line-height: 1.75em;\">1990\u5e74\u590d\u65e6\u5927\u5b66\u7b49\u8fdb\u884c\u4e86\u4e16\u754c\u9996\u6b21\u8840\u53cb\u75c5B\u57fa\u56e0\u6cbb\u7597\u3002\u5362\u5927\u5112\u6559\u6388\u8bf4\u9053\uff1a\u201c\u5f53\u5e74\u628a\u57fa\u56e0\u7597\u6cd5\u5f15\u5165\u5927\u5bb6\u89c6\u91ce\u662f\u9488\u5bf9\u9057\u4f20\u75c5\u7684\u57fa\u56e0\u6cbb\u7597\uff0c\u4f46\u662f\u8ba9\u5176\u771f\u6b63\u53d1\u5c55\u3001\u843d\u5730\u662f\u901a\u8fc7\u5bf9\u80bf\u7624\u7684\u6cbb\u7597\uff0c\u4f8b\u5982CAR-T\u7597\u6cd5\u2014\u2014\u5df2\u5728\u767d\u8840\u75c5\u9886\u57df\u53d6\u5f97\u4e86\u663e\u8457\u6210\u6548\u3002\u5728\u5305\u62ec\u5730\u4e2d\u6d77\u8d2b\u8840\u3001\u9ed1\u6726\u75c5\u3001\u5148\u5929\u6027\u7684\u8054\u5408\u514d\u75ab\u7f3a\u9677\u75c7\u7b49\u5728\u5185\u7684\u9057\u4f20\u75c5\u6cbb\u7597\u65b9\u9762\uff0c\u57fa\u56e0\u7597\u6cd5\u540c\u6837\u4e5f\u53d6\u5f97\u4e86\u826f\u597d\u7684\u6210\u7ee9\uff0c\u8fd9\u4e9b\u8fdb\u5c55\u6781\u5927\u5730\u9f13\u821e\u4e86\u79d1\u5b66\u5de5\u4f5c\u8005\u7684\u4fe1\u5fc3\uff01\u201d<\/p>\n<section class=\"\"  style=\"white-space: normal;max-width: 100%;color: rgb(51, 51, 51);\">\n<section style=\"margin: 10px auto;max-width: 100%;text-align: center;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section style=\"margin-bottom: 3px;margin-left: 33px;max-width: 100%;font-size: 0px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"margin-bottom: 10px;max-width: 100%;width: 6px;height: 6px;display: inline-block;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><span style=\"max-width: 100%;display: inline-block;width: 10px;height: 10px;opacity: 0.6;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span>&nbsp;&nbsp;&nbsp;<\/span><\/section>\n<section style=\"margin-top: -2px;max-width: 100%;font-size: 0px;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;display: inline-block;width: 15px;height: 15px;border-width: 1px;border-style: solid;border-color: white;opacity: 0.9;vertical-align: bottom;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;display: inline-block;border-width: 1px;border-style: solid;border-color: rgb(255, 255, 255);width: 30px;height: 30px;line-height: 30px;vertical-align: top;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\">\n<p class=\"\" style=\"margin-right: auto;margin-left: auto;max-width: 100%;min-height: 1em;white-space: pre-wrap;min-width: 1px;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;\">03<\/p>\n<\/section>\n<section style=\"max-width: 100%;display: inline-block;width: 15px;height: 15px;border-width: 1px;border-style: solid;border-color: white;opacity: 0.9;vertical-align: top;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/section>\n<\/section>\n<section style=\"margin-top: 3px;margin-left: -33px;max-width: 100%;font-size: 0px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;display: inline-block;width: 10px;height: 10px;opacity: 0.6;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><span style=\"margin-bottom: -10px;max-width: 100%;width: 6px;height: 6px;display: inline-block;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span>&nbsp; &nbsp;<\/span><\/section>\n<\/section>\n<\/section>\n<p style=\"white-space: normal;max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);text-align: center;\"><span style=\"max-width: 100%;font-size: 16px;color: rgb(123, 12, 0);box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;text-align: justify;box-sizing: border-box !important;word-wrap: break-word !important;\">\u9057\u4f20\u54a8\u8be2\u7684\u672a\u6765<\/span><\/strong><\/span><\/p>\n<p style=\"white-space: normal;max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><\/p>\n<p style=\"white-space: normal;text-align: justify;line-height: 1.75em;\">\u5f53\u63d0\u53ca\u56fd\u5185\u9057\u4f20\u54a8\u8be2\u7684\u73b0\u72b6\u548c\u5bf9\u672a\u6765\u56fd\u5185\u9057\u4f20\u54a8\u8be2\u7684\u5c55\u671b\u65f6\uff0c\u5362\u5927\u5112\u6559\u6388\u8fd9\u6837\u8bf4\u9053\uff1a\u201c\u73b0\u5728\uff0c\u56fd\u5185\u9057\u4f20\u54a8\u8be2\u975e\u5e38\u706b\u7206\uff0c\u98ce\u8d77\u4e91\u6d8c\uff0c\u5404\u8def\u8bf8\u4faf\u7eb7\u7eb7\u505a\u9057\u4f20\u54a8\u8be2\uff0c\u8fd9\u662f\u56e0\u4e3a\u6709\u9700\u6c42\u3002\u7279\u522b\u662f\u57fa\u56e0\u6d4b\u5e8f\u65f6\u4ee3\u7684\u66f4\u8fed\uff0c\u89e3\u8bfb\u57fa\u56e0\u7ec4\u4fe1\u606f\u9010\u6e10\u6210\u4e3a\u521a\u9700\u3002\u81ea2013\u5e74\u5f00\u59cb\uff0c\u590d\u65e6\u5927\u5b66\u5148\u540e\u4e3e\u529e\u4e86\u4e24\u5c4a\u9057\u4f20\u54a8\u8be2\u57f9\u8bad\u73ed\uff0c\u5171\u57f9\u517b\u4e86\u7ea680\u540d\u9057\u4f20\u54a8\u8be2\u5e08\u30022015\u5e74\uff0c\u4e2d\u56fd\u9057\u4f20\u534f\u4f1a\u9057\u4f20\u54a8\u8be2\u5206\u4f1a\u6b63\u5f0f\u6210\u7acb\uff0c\u5230\u76ee\u524d\u5df2\u6210\u529f\u4e3e\u529e\u5341\u591a\u5c4a\u9057\u4f20\u54a8\u8be2\u5e08\u57f9\u8bad\u73ed\uff0c\u4e3a\u5f88\u591a\u533b\u9662\u9057\u4f20\u5de5\u4f5c\u8005\u63d0\u4f9b\u4e86\u5f88\u597d\u7684\u6559\u80b2\u3001\u57f9\u8bad\u673a\u4f1a\u3002\u6211\u4eec\u6b22\u8fce\u66f4\u591a\u4eba\u52a0\u5165\u9057\u4f20\u5b66\u7684\u6559\u80b2\u4e2d\uff0c\u540c\u65f6\uff0c\u6211\u4eec\u5e0c\u671b\u9057\u4f20\u5b66\u7684\u6559\u80b2\u5728\u4e0e\u56fd\u9645\u63a5\u8f68\u7684\u8fc7\u7a0b\u4e2d\u9010\u6b65\u89c4\u8303\u5316\u3002\u201d<\/p>\n<p style=\"white-space: normal;text-align: justify;line-height: 1.75em;\">&nbsp;<\/p>\n<p style=\"white-space: normal;text-align: justify;line-height: 1.75em;\">\u5bf9\u4e8e\u9057\u4f20\u54a8\u8be2\u7684\u672a\u6765\uff0c\u5362\u5927\u5112\u6559\u6388\u5c55\u671b\u9053\uff0c\u201c\u9996\u5148\uff0c\u5e0c\u671b\u666e\u53ca\u5230\u4e34\u5e8a\u533b\u751f\u3001\u57fa\u56e0\u6570\u636e\u5206\u6790\u4eba\u5458\u3002\u7b2c\u4e8c\uff0c\u9057\u4f20\u54a8\u8be2\u80fd\u591f\u8ddf\u9ad8\u79d1\u6280\u7ed3\u5408\uff0c\u6bd4\u5982\u4eba\u5de5\u667a\u80fd\u3001\u5927\u6570\u636e\u3001APP\u7b49\u3002\u540c\u65f6\uff0c\u9057\u4f20\u54a8\u8be2\u79bb\u4e0d\u5f00\u6570\u636e\u5e93\uff0c\u8fd9\u662f\u54a8\u8be2\u7684\u4f9d\u636e\uff0c\u6240\u4ee5\u8981\u642d\u5efa\u5f88\u597d\u7684\u516c\u5171\u6570\u636e\u5e93\u3002\u6b64\u5916\uff0c\u8fd8\u9700\u8981\u4e2d\u56fd\u9057\u4f20\u5b66\u4f1a\u9057\u4f20\u54a8\u8be2\u5206\u4f1a\u3001\u4e2d\u534e\u533b\u5b66\u4f1a\u9057\u4f20\u5b66\u5206\u4f1a\u7b49\u51fa\u53f0\u4e00\u4e9b\u6307\u5357\u548c\u4e13\u5bb6\u5171\u8bc6\uff0c\u63d0\u4f9b\u6307\u5bfc\u3002\u201d<\/p>\n<section class=\"\"  style=\"white-space: normal;max-width: 100%;color: rgb(51, 51, 51);\">\n<section style=\"margin: 10px auto;max-width: 100%;text-align: center;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section style=\"margin-bottom: 3px;margin-left: 33px;max-width: 100%;font-size: 0px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"margin-bottom: 10px;max-width: 100%;width: 6px;height: 6px;display: inline-block;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><span style=\"max-width: 100%;display: inline-block;width: 10px;height: 10px;opacity: 0.6;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span>&nbsp;&nbsp;&nbsp;<\/span><\/section>\n<section style=\"margin-top: -2px;max-width: 100%;font-size: 0px;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;display: inline-block;width: 15px;height: 15px;border-width: 1px;border-style: solid;border-color: white;opacity: 0.9;vertical-align: bottom;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;display: inline-block;border-width: 1px;border-style: solid;border-color: rgb(255, 255, 255);width: 30px;height: 30px;line-height: 30px;vertical-align: top;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\">\n<p class=\"\" style=\"margin-right: auto;margin-left: auto;max-width: 100%;min-height: 1em;white-space: pre-wrap;min-width: 1px;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;\">04<\/p>\n<\/section>\n<section style=\"max-width: 100%;display: inline-block;width: 15px;height: 15px;border-width: 1px;border-style: solid;border-color: white;opacity: 0.9;vertical-align: top;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/section>\n<\/section>\n<section style=\"margin-top: 3px;margin-left: -33px;max-width: 100%;font-size: 0px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;display: inline-block;width: 10px;height: 10px;opacity: 0.6;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><span style=\"margin-bottom: -10px;max-width: 100%;width: 6px;height: 6px;display: inline-block;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span>&nbsp; &nbsp;<\/span><\/section>\n<\/section>\n<\/section>\n<p style=\"white-space: normal;max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);text-align: center;\"><span style=\"max-width: 100%;font-size: 16px;color: rgb(123, 12, 0);box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;text-align: justify;box-sizing: border-box !important;word-wrap: break-word !important;\">\u6b23\u8d4f\u751f\u547d\u79d1\u5b66\u7684\u98ce\u666f<\/span><\/strong><\/span><\/p>\n<p style=\"white-space: normal;text-align: justify;line-height: 1.75em;\">&nbsp;<\/p>\n<p style=\"white-space: normal;text-align: justify;line-height: 1.75em;\">\u201c\u7b2c\u4e00\u8eab\u4efd\u662f\u8001\u5e08\uff0c\u7b2c\u4e8c\u8eab\u4efd\u662f\u6709\u5174\u8da3\u7684\u9057\u4f20\u79d1\u7814\u5de5\u4f5c\u8005\u201d\u3002\u8fd9\u662f\u5362\u5927\u5112\u6559\u6388\u5bf9\u81ea\u5df1\u7684\u8eab\u4efd\u5b9a\u4f4d\u3002\u4ed6\u7b11\u7740\u8bf4\uff0c\u201c\u6211\u8ddf\u5f88\u591a\u79d1\u7814\u5de5\u4f5c\u8005\u4e0d\u592a\u4e00\u6837\uff0c\u6211\u5bf9\u79d1\u7814\u7684\u5de5\u4f5c\u662f\u5174\u8da3\u9a71\u52a8\u3002\u6211\u628a\u8fd9\u79cd\u81ea\u7531\u63a2\u7d22\u7406\u89e3\u4e3a\u5728\u751f\u547d\u79d1\u5b66\u7684\u98ce\u666f\u91cc\u9762\u8fdb\u884c\u6b23\u8d4f\u3002\u4f5c\u4e3a\u4e00\u4f4d\u8001\u5e08\uff0c\u6211\u8981\u626e\u6f14\u2018\u5bfc\u6e38\u2019\u7684\u89d2\u8272\uff0c\u5e26\u9886\u5b66\u751f\u4eec\u53bb\u6b23\u8d4f\u548c\u9274\u522b\u98ce\u666f\uff0c\u79d1\u5b66\u7814\u7a76\u4e5f\u662f\u6709\u54c1\u4f4d\u7684\uff0c\u8fd9\u662f\u6559\u5e08\u5e94\u8be5\u5177\u5907\u7684\u7d20\u8d28\uff0c\u77e5\u8bc6\u9762\u6bd4\u8f83\u5e7f\u535a\uff0c\u5bf9\u65f6\u4ee3\u7684\u524d\u6cbf\u628a\u63e1\u6bd4\u8f83\u51c6\uff0c\u5728\u6b64\u57fa\u7840\u4e0a\u518d\u505a\u7814\u7a76\u3002\u6b64\u5916\uff0c\u6211\u5bf9\u6280\u672f\u4e5f\u5f88\u611f\u5174\u8da3\uff0c\u6280\u672f\u662f\u63a8\u52a8\u79d1\u5b66\u53d1\u5c55\u7684\u52a8\u529b\u3002\u4fd7\u8bdd\u8bf4\uff0c\u78e8\u5200\u4e0d\u8bef\u780d\u67f4\u5de5\uff0c\u6709\u4e86\u597d\u7684\u6280\u672f\u53ef\u4ee5\u4e8b\u534a\u529f\u500d\u3002\u6240\u4ee5\u6211\u7ecf\u5e38\u9f13\u52b1\u540c\u5b66\u4eec\u8981\u6709\u5de5\u5320\u7cbe\u795e\uff0c\u5bf9\u6280\u672f\u8981\u94bb\u7814\u3002\u201d<\/p>\n<p style=\"white-space: normal;text-align: justify;line-height: 1.75em;\">&nbsp;<\/p>\n<p style=\"white-space: normal;text-align: justify;line-height: 1.75em;\">\u91c7\u8bbf\u6700\u540e\uff0c\u5362\u5927\u5112\u6559\u6388\u8868\u793a\uff0c\u8eab\u4e3a\u6cf0\u5dde\u4eba\uff0c\u5bf9\u4e8e\u8fd1\u5e74\u6765\u5bb6\u4e61\u533b\u836f\u4ea7\u4e1a\u7684\u53d8\u5316\u6709\u7740\u7279\u6b8a\u7684\u611f\u53d7\u3002\u201c\u4e09\u5e74\u4ee5\u540e\uff0c\u518d\u6b21\u56de\u5230\u6cf0\u5dde\u53c2\u52a0\u57fa\u56e0\u68c0\u6d4b\u4e0e\u5065\u5eb7\u4ea7\u4e1a\u5927\u4f1a\uff0c\u975e\u5e38\u9ad8\u5174\u770b\u5230\u4e2d\u56fd\u533b\u836f\u57ce\u7684\u817e\u98de\u3002\u98de\u901f\u53d1\u5c55\u7684\u80cc\u540e\u80af\u5b9a\u79bb\u4e0d\u5f00\u5f53\u5730\u653f\u5e9c\u5bf9\u8f85\u52a9\u670d\u52a1\u3001\u4eba\u624d\u5f15\u8fdb\u7b49\u65b9\u9762\u7684\u52aa\u529b\u5de5\u4f5c\u3002\u6211\u5e0c\u671b\uff0c\u672a\u6765\u4e2d\u56fd\u533b\u836f\u57ce\u66f4\u52a0\u7ea2\u706b\uff0c\u80fd\u591f\u771f\u6b63\u8fbe\u5230\u5728\u4e2d\u56fd\u533b\u836f\u7814\u53d1\u3001\u6d41\u901a\u7b49\u591a\u65b9\u9762\u771f\u6b63\u9886\u5148\u7684\u6c34\u5e73\u3002\u201d\u4ed6\u5982\u6b64\u671f\u5f85\u3002<\/p>\n<p style=\"white-space: normal;text-align: justify;line-height: 1.75em;\"><\/p>\n<p style=\"white-space: normal;text-align: right;line-height: 1.75em;\"><strong>\u8d23\u7f16\uff1a\u6d6e\u82cf<\/strong><\/p>\n<section  class=\"\" style=\"white-space: normal;max-width: 100%;color: rgb(51, 51, 51);\">\n<section  style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section class=\"\" style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section  class=\"\" style=\"max-width: 100%;line-height: 28.4444px;font-family: \u5fae\u8f6f\u96c5\u9ed1;border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box !important;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<section  style=\"margin: 1em auto;max-width: 100%;border-width: 0px;border-style: none;border-color: initial;text-align: center;width: 941px;box-sizing: border-box !important;word-wrap: break-word !important;overflow-wrap: break-word !important;\"><span class=\"\"  style=\"max-width: 100%;width: 941px;border-bottom: 1px solid rgb(172, 29, 16);border-top-color: rgb(172, 29, 16);border-right-color: rgb(172, 29, 16);border-left-color: rgb(172, 29, 16);display: inline-block;box-sizing: border-box !important;word-wrap: break-word !important;overflow-wrap: break-word !important;\"><\/p>\n<section class=\"\" style=\"margin-bottom: -3px;padding-top: 5px;padding-right: 10px;padding-left: 10px;max-width: 100%;display: inline-block;min-height: 32px;border-bottom: 5px solid rgb(172, 29, 16);font-size: 20px;color: rgb(121, 121, 121);box-sizing: border-box !important;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<p class=\"\" style=\"margin-top: 5px;margin-bottom: 5px;max-width: 100%;min-height: 1em;line-height: 1.6em;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;overflow-wrap: break-word !important;\">End<\/p>\n<\/section>\n<p><\/span><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section  class=\"\" style=\"font-size: 16px;white-space: normal;max-width: 100%;box-sizing: border-box;letter-spacing: 0.544px;text-align: justify;background-color: rgb(255, 255, 255);color: rgb(62, 62, 62);line-height: 25.6px;font-family: \u5fae\u8f6f\u96c5\u9ed1;border-width: 0px;border-style: none;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<section class=\"\"  style=\"padding: 2px;max-width: 100%;box-sizing: border-box;border-width: 1px;border-style: dotted;border-color: rgb(218, 98, 71);word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<section class=\"\" style=\"margin: 5px auto;max-width: 100%;box-sizing: border-box;border-width: 0px;border-style: none;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<section class=\"\" style=\"max-width: 100%;box-sizing: border-box;height: 25px;color: inherit;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<section class=\"\" style=\"max-width: 100%;box-sizing: border-box;height: 25px;width: 50px;float: left;border-top: 2px solid rgb(172, 29, 16);border-right-color: rgb(172, 29, 16);border-bottom-color: rgb(172, 29, 16);border-left: 2px solid rgb(172, 29, 16);color: inherit;word-wrap: break-word !important;overflow-wrap: break-word !important;\"><\/section>\n<section class=\"\" style=\"max-width: 100%;box-sizing: border-box;display: inline-block;color: rgb(172, 29, 16);clear: both;border-color: rgb(172, 29, 16);word-wrap: break-word !important;overflow-wrap: break-word !important;\"><\/section>\n<\/section>\n<section class=\"\"  style=\"margin: -0.8em 0.3em;padding: 0.8em;max-width: 100%;box-sizing: border-box;color: inherit;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<p class=\"\" style=\"max-width: 100%;min-height: 1em;overflow-wrap: break-word;color: rgb(51, 51, 51);font-size: 1em;line-height: 1.75em;word-break: break-all;box-sizing: border-box !important;word-wrap: break-word !important;\"><span class=\"\" style=\"max-width: 100%;color: rgb(0, 0, 0);line-height: 22.4px;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\u672c\u6587\u7cfb\u751f\u7269\u63a2\u7d22\u539f\u521b\uff0c\u6b22\u8fce\u4e2a\u4eba\u8f6c\u53d1\u5206\u4eab\u3002\u5176\u4ed6\u4efb\u4f55\u5a92\u4f53\u3001\u7f51\u7ad9\u5982\u9700\u8f6c\u8f7d\uff0c\u987b\u5728\u6b63\u6587\u524d\u6ce8\u660e\u6765\u6e90\u751f\u7269\u63a2\u7d22\u3002<\/span><\/p>\n<\/section>\n<section class=\"\" style=\"max-width: 100%;box-sizing: border-box;height: 25px;color: inherit;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<section class=\"\" style=\"max-width: 100%;box-sizing: border-box;height: 25px;width: 50px;float: right;border-bottom: 2px solid rgb(172, 29, 16);border-top-color: rgb(172, 29, 16);border-right: 2px solid rgb(172, 29, 16);border-left-color: rgb(172, 29, 16);color: inherit;word-wrap: break-word !important;overflow-wrap: break-word !important;\"><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p class=\"\" style=\"white-space: normal;max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><img class=\"\"       width=\"auto\" style=\"box-sizing: border-box !important;word-wrap: break-word !important;visibility: visible !important;width: auto !important;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/08\/beepress9-1533788158.png\" alt=\"\u4e13\u8bbf | \u590d\u65e6\u5927\u5b66\u5362\u5927\u5112\u6559\u6388\uff1a\u57fa\u56e0\u7f16\u8f91\u6b63\u5f53\u65f6\uff0c\u5404\u8272\u5e94\u7528\u663e\u8eab\u624b\" title=\"\u4e13\u8bbf | \u590d\u65e6\u5927\u5b66\u5362\u5927\u5112\u6559\u6388\uff1a\u57fa\u56e0\u7f16\u8f91\u6b63\u5f53\u65f6\uff0c\u5404\u8272\u5e94\u7528\u663e\u8eab\u624b\"><\/p>\n<blockquote class='keep-source'>\n<p>\u59cb\u53d1\u4e8e\u5fae\u4fe1\u516c\u4f17\u53f7\uff1a                         \u751f\u7269\u63a2\u7d22                      <\/p>\n<\/blockquote>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u751f\u7269\u63a2\u7d22 \u7f16\u8005\u6309 \u4ed6\u5728\u590d\u65e6\u5927\u5b66\u751f\u547d\u79d1\u5b66\u5b66\u9662\u9ed8\u9ed8\u575a\u5b88\u4e8634\u5e74\uff0c\u5e26\u9886\u7814\u7a76\u56e2\u961f\u5728\u8840\u53cb\u75c5\u3001\u80bf\u7624\u7b49\u75be\u75c5\u7684\u9057\u4f20\u5b66\u7814\u7a76\u65b9\u9762\u53d6 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":31400,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[2],"tags":[],"_links":{"self":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/31399"}],"collection":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/comments?post=31399"}],"version-history":[{"count":1,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/31399\/revisions"}],"predecessor-version":[{"id":31406,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/31399\/revisions\/31406"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media\/31400"}],"wp:attachment":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media?parent=31399"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/categories?post=31399"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/tags?post=31399"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}