{"id":31468,"date":"2018-08-04T01:00:00","date_gmt":"2018-08-09T04:16:29","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/2018\/08\/09\/%e9%ad%94%e5%89%aa%e5%89%aa%e5%88%87%e5%90%8e%ef%bc%8c%e7%bb%86%e8%83%9e%e5%a6%82%e4%bd%95%e4%bf%ae%e5%a4%8d%ef%bc%9fnature%e5%ad%90%e5%88%8a%ef%bc%9a%e6%b2%a1%e9%82%a3%e4%b9%88\/"},"modified":"2018-08-09T12:16:32","modified_gmt":"2018-08-09T04:16:32","slug":"%e9%ad%94%e5%89%aa%e5%89%aa%e5%88%87%e5%90%8e%ef%bc%8c%e7%bb%86%e8%83%9e%e5%a6%82%e4%bd%95%e4%bf%ae%e5%a4%8d%ef%bc%9fnature%e5%ad%90%e5%88%8a%ef%bc%9a%e6%b2%a1%e9%82%a3%e4%b9%88","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/31468\/","title":{"rendered":"\u201c\u9b54\u526a\u201d\u526a\u5207\u540e\uff0c\u7ec6\u80de\u5982\u4f55\u4fee\u590d\uff1fNature\u5b50\u520a\uff1a\u6ca1\u90a3\u4e48\u7b80\u5355"},"content":{"rendered":"<div class=\"bpp-post-content\">\n<section class=\"xmteditor\" style=\"display:none;\"  ><\/section>\n<section class=\"\" label=\"Copyright \u00a9 2015 Yead All Rights Reserved.\" style=\"max-width: 100%;color: rgb(51, 51, 51);\">\n<fieldset style=\"margin-top: 0.5em;margin-bottom: 0.5em;max-width: 100%;box-sizing: border-box;min-width: 0px;border-width: 0px;border-style: initial;border-color: initial;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<section class=\"\" style=\"margin-left: 5.51563px;max-width: 100%;box-sizing: border-box;border-width: 1px;border-style: solid;border-color: rgb(197, 63, 70);border-radius: 0px 5px 5px 0px;font-size: 1em;font-family: inherit;text-align: inherit;text-decoration: inherit;color: rgb(52, 54, 60);word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<section class=\"\" style=\"margin-top: 27.2656px;margin-right: 8px;margin-left: -8px;max-width: 100%;box-sizing: border-box;float: left;font-size: 1em;font-family: inherit;font-style: inherit;text-decoration: inherit;color: rgb(255, 255, 255);border-color: rgb(197, 63, 70);word-wrap: break-word !important;overflow-wrap: break-word !important;\"><span class=\"\" style=\"padding: 0.3em 0.5em;max-width: 100%;box-sizing: border-box;display: inline-block;border-radius: 0px 0.5em 0.5em 0px;font-size: 1em;font-family: inherit;background-color: rgb(197, 63, 70);word-wrap: break-word !important;overflow-wrap: break-word !important;\"><\/p>\n<section style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\u751f\u7269\u63a2\u7d22<\/section>\n<p><\/span><\/p>\n<section class=\"\" style=\"max-width: 100%;box-sizing: border-box;width: 0px;border-right: 4px solid rgb(197, 63, 70);border-top: 4px solid rgb(197, 63, 70);word-wrap: break-word !important;overflow-wrap: break-word !important;border-left: 4px solid transparent !important;border-bottom: 4px solid transparent !important;\"><\/section>\n<\/section>\n<section style=\"margin-top: 27.2656px;padding-right: 8px;padding-left: 8px;max-width: 100%;box-sizing: border-box;font-size: 1.5em;font-family: inherit;text-align: inherit;text-decoration: inherit;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;overflow-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 18px;box-sizing: border-box !important;word-wrap: break-word !important;overflow-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\u7f16\u8005\u6309<\/strong><\/span><\/section>\n<\/section>\n<section style=\"max-width: 100%;box-sizing: border-box;clear: both;word-wrap: break-word !important;overflow-wrap: break-word !important;\"><\/section>\n<section style=\"padding: 8px;max-width: 100%;box-sizing: border-box;line-height: 30px;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<p style=\"max-width: 100%;min-height: 1em;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">CRISPR\u81ea\u95ee\u4e16\u4ee5\u6765\u4e00\u76f4\u88ab\u5bc4\u4e88\u539a\u671b\uff0c\u4f46\u662f\u5bf9\u4e8e\u5b83\u5728\u7ec6\u80de\u4e2d\u7684\u4f5c\u7528\u7ec6\u8282\uff0c\u4f9d\u7136\u6709\u5f88\u591a\u672a\u77e5\u3002\u8fd1\u65e5\uff0c\u79d1\u5b66\u5bb6\u4eec\u53d1\u73b0\uff0c\u8fc7\u53bb\u5bf9\u4e8e\u201c\u7ec6\u80de\u5728Cas9\u9176\u526a\u5207DNA\u540e\u4fee\u590d\u57fa\u56e0\u201d\u7684\u5047\u8bbe\u662f\u9519\u8bef\u7684\uff0c\u7ec6\u80de\u4fee\u590d\u201c\u9b54\u526a\u201d\u9020\u6210\u7684DNA\u65ad\u88c2\u5b8c\u5168\u4e0d\u540c\u4e8e\u6700\u521d\u7684\u5047\u8bbe\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/fieldset>\n<\/section>\n<p style=\"line-height: 1.75em;text-align: center;\"><img class=\"\"     style=\"margin-top: 6px;margin-bottom: 6px;border-width: 0px;border-style: none;border-color: initial;vertical-align: middle;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/08\/beepress1-1533788190-1.jpeg\" alt=\"\u201c\u9b54\u526a\u201d\u526a\u5207\u540e\uff0c\u7ec6\u80de\u5982\u4f55\u4fee\u590d\uff1fNature\u5b50\u520a\uff1a\u6ca1\u90a3\u4e48\u7b80\u5355\" title=\"\u201c\u9b54\u526a\u201d\u526a\u5207\u540e\uff0c\u7ec6\u80de\u5982\u4f55\u4fee\u590d\uff1fNature\u5b50\u520a\uff1a\u6ca1\u90a3\u4e48\u7b80\u5355\"  \/><br  \/><\/p>\n<p style=\"line-height: 1.75em;text-align: center;\"><span style=\"color: rgb(136, 136, 136);\">IrvineNIST<\/span><br  \/><\/p>\n<p style=\"line-height: 1.75em;\"><br  \/><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\">7\u670827\u65e5\uff0c\u300aNature Genetics\u300b\u671f\u520a\u6700\u65b0\u53d1\u8868\u4e86\u8fd9\u7bc7\u9898\u4e3a\u201cCRISPR\u2013Cas9 genome editing in human cells occurs via the Fanconi anemia pathway\u201d\u6587\u7ae0\u3002\u6765\u81ea\u4e8e\u52a0\u5dde\u5927\u5b66\u4f2f\u514b\u5229\u5206\u6821\u7684\u79d1\u5b66\u5bb6\u4eec\u53d1\u73b0\uff0c\u4e00\u6761\u7f55\u89c1\u7684DNA\u4fee\u590d\u9014\u5f84\u2014\u2014<strong>\u8303\u53ef\u5c3c\u8d2b\u8840\u901a\u8def\uff08Fanconi anemia pathway\uff09\u5728\u4fee\u590d\u7531\u201c\u9b54\u526a\u201dCRISPR-Cas9\u7f16\u8f91\u9020\u6210\u7684\u53cc\u94feDNA\u65ad\u88c2\u8fc7\u7a0b\u4e2d\u53d1\u6325\u7740\u5173\u952e\u4f5c\u7528\u3002<\/strong>\u800c\u4e14\uff0c\u8303\u53ef\u5c3c\u8d2b\u8840\u901a\u8def\u7c7b\u4f3c\u4e8e\u201c\u4ea4\u901a\u8b66\u5bdf\u201d\uff0c\u5f15\u5bfc\u7b80\u5355\u7684\u672b\u7aef\u8fde\u63a5\u6216\u8005\u65b0\u7684\u5355\u94feDNA\u4f24\u53e3\u4fee\u8865\u3002<\/p>\n<p><br  \/><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\">\u8fd9\u9879\u53d1\u73b0\u63ed\u793a\u4e86\u4e3a\u4ec0\u4e48CRISPR-Cas9\u6280\u672f\u51e0\u4e4e\u5728\u6240\u6709\u5c1d\u8bd5\u8fc7\u7684\u7ec6\u80de\u4e2d\u90fd\u975e\u5e38\u6709\u6548\uff08\u867d\u7136\u5e76\u4e0d\u662f\u6240\u6709\u7ec6\u80de\u90fd\u83b7\u5f97\u76f8\u540c\u6c34\u5e73\u7684\u6210\u529f\uff09\uff0c\u6709\u52a9\u4e8e\u5e2e\u52a9\u79d1\u7814\u4eba\u5458\u63d0\u9ad8\u5728\u57fa\u56e0\u7ec4\u4e2d\u63d2\u5165\u65b0DNA\uff08\u4f8b\u5982\u7528\u6b63\u786e\u7684DNA\u5e8f\u5217\u66ff\u6362\u6709\u5bb3\u7684\u7a81\u53d8\uff09\u7684\u6548\u7387\uff0c\u5e76\u786e\u4fddCRISPR\u7f16\u8f91\u83b7\u5f97\u9884\u671f\u7684\u7ed3\u679c\u3002<\/p>\n<p><br  \/><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\">\u201c\u57fa\u56e0\u7f16\u8f91\u6f5c\u529b\u5f88\u5927\uff0c\u4f46\u662f\u5f53\u524d\u9762\u4e34\u5f88\u591a\u98ce\u9669\u548c\u9519\u8bef\u3002\u201d\u6587\u7ae0\u4f5c\u8005\u3001\u52a0\u5dde\u5927\u5b66\u4f2f\u514b\u5229\u5206\u6821\u5206\u5b50\u548c\u7ec6\u80de\u751f\u7269\u5b66\u6559\u6388Jacob Corn\u5f3a\u8c03\uff0c<strong>\u201c\u5b83\u5728\u4eba\u7c7b\u7ec6\u80de\u4e2d\u7684\u5de5\u4f5c\u65b9\u5f0f\u4e00\u76f4\u662f\u4e00\u4e2a\u6709\u7740\u5f88\u591a\u5047\u8bbe\u7684\u2018\u9ed1\u5323\u5b50\u2019\u3002\u73b0\u5728\uff0c\u6211\u4eec\u7684\u5de5\u4f5c\u662f\u5f00\u59cb\u89e3\u5f00\u8fd9\u4e00\u8c1c\u56e2\u3002\u201d<\/strong><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><br  \/><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><img class=\"\"     style=\"margin-top: 6px;margin-bottom: 6px;border-width: 0px;border-style: none;border-color: initial;vertical-align: middle;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/08\/beepress3-1533788190.jpeg\" alt=\"\u201c\u9b54\u526a\u201d\u526a\u5207\u540e\uff0c\u7ec6\u80de\u5982\u4f55\u4fee\u590d\uff1fNature\u5b50\u520a\uff1a\u6ca1\u90a3\u4e48\u7b80\u5355\" title=\"\u201c\u9b54\u526a\u201d\u526a\u5207\u540e\uff0c\u7ec6\u80de\u5982\u4f55\u4fee\u590d\uff1fNature\u5b50\u520a\uff1a\u6ca1\u90a3\u4e48\u7b80\u5355\"  \/><br  \/><\/p>\n<section class=\"\"  style=\"max-width: 100%;color: rgb(51, 51, 51);\">\n<section style=\"margin: 10px auto;max-width: 100%;text-align: center;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section style=\"margin-bottom: 3px;margin-left: 33px;max-width: 100%;font-size: 0px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"margin-bottom: 10px;max-width: 100%;width: 6px;height: 6px;display: inline-block;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><span style=\"max-width: 100%;display: inline-block;width: 10px;height: 10px;opacity: 0.6;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span>&nbsp;&nbsp;&nbsp;<\/span><\/section>\n<section style=\"margin-top: -2px;max-width: 100%;font-size: 0px;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;display: inline-block;width: 15px;height: 15px;border-width: 1px;border-style: solid;border-color: white;opacity: 0.9;vertical-align: bottom;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;display: inline-block;border-width: 1px;border-style: solid;border-color: rgb(255, 255, 255);width: 30px;height: 30px;line-height: 30px;vertical-align: top;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\">\n<p class=\"\" style=\"margin-right: auto;margin-left: auto;max-width: 100%;min-height: 1em;white-space: pre-wrap;min-width: 1px;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;\">01<\/p>\n<\/section>\n<section style=\"max-width: 100%;display: inline-block;width: 15px;height: 15px;border-width: 1px;border-style: solid;border-color: white;opacity: 0.9;vertical-align: top;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/section>\n<\/section>\n<section style=\"margin-top: 3px;margin-left: -33px;max-width: 100%;font-size: 0px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;display: inline-block;width: 10px;height: 10px;opacity: 0.6;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><span style=\"margin-bottom: -10px;max-width: 100%;width: 6px;height: 6px;display: inline-block;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span>&nbsp; &nbsp;<\/span><\/section>\n<\/section>\n<\/section>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);text-align: center;\"><span style=\"max-width: 100%;font-size: 16px;color: rgb(123, 12, 0);box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;text-align: justify;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"text-align: justify;\">CRISPR\u4f9d\u8d56\u4e8eDNA\u4fee\u590d<\/span><\/span><\/span><\/strong><\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br  \/><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\">CRISPR-Cas9\u662f\u4e00\u9879\u9769\u547d\u6027\u6280\u672f\uff0c\u80fd\u591f\u4ece\u6570\u5341\u4ebf\u57fa\u56e0\u7ec4\u4e2d\u7cbe\u786e\u5730\u63d0\u53d6\u51fa\u7279\u5b9a\u7684DNA\u3002\u7f16\u8f91\u5de5\u4f5c\u4e4b\u540e\uff0c\u5c31\u662f<strong>\u7ec6\u80de\u7684\u81ea\u6211\u4fee\u590d\u4e86<\/strong>\u3002\u901a\u5e38\uff0cDNA\u4fee\u590d\u53ef\u901a\u8fc7\u4e24\u79cd\u65b9\u5f0f\u8fdb\u884c\uff1a<\/p>\n<p><br  \/><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><strong>1\uff09\t\u975e\u540c\u6e90\u6027\u672b\u7aef\u8fde\u63a5\uff08non-homologous end-joining\uff09<\/strong>\uff0c\u901a\u8fc7\u7279\u5b9a\u7684\u9176\u5c06\u5207\u5f00\u7684\u672b\u7aef\u7f1d\u5408\u5728\u4e00\u8d77\uff0c\u8fd9\u4e00\u65b9\u5f0f\u901a\u5e38\u4f1a\u5bfc\u81f4\u4e00\u4e2a\u6216\u591a\u4e2a\u78b1\u57fa\u88ab\u6dfb\u52a0\u6216\u5220\u9664\uff0c\u4ece\u800c\u5bb9\u6613\u7834\u574f\u57fa\u56e0\u529f\u80fd\uff0c\u8d77\u5230\u201c\u57fa\u56e0\u6572\u9664\u201d\u7684\u6548\u679c\u3002\u8fd9\u662fCRISPR\u5207\u5272\u540e\u6700\u5e38\u89c1\u7684\u7ed3\u679c\uff1b<\/p>\n<p><br  \/><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><strong>2\uff09\t\u540c\u6e90\u4ecb\u5bfc\u4fee\u590d\uff08homology-directed repair\uff09\uff0c<\/strong>\u5229\u7528\u5355\u94feDNA\u6a21\u677f\uff0c\u5f15\u5165\u65b0\u7684\u5e8f\u5217\uff0c\u5bf9\u57fa\u56e0\u8fdb\u884c\u7f16\u8f91\u3002\u8fd9\u4e00\u65b9\u5f0f\u5728\u67d0\u4e9b\u7c7b\u578b\u7684\u7ec6\u80de\u4e2d\u53d1\u751f\u7684\u9891\u7387\u6bd4\u5176\u4ed6\u7c7b\u578b\u7684\u7ec6\u80de\u8981\u9ad8\uff0c\u5e76\u4e14\u9700\u8981\u4e00\u6bb5\u53ef\u4ee5\u7528\u6765\u4fee\u8865\u5207\u53e3\u7684DNA\u7247\u6bb5\u3002\u7814\u7a76\u4eba\u5458\u7ecf\u5e38\u63d0\u4f9b\u5355\u94feDNA\u7247\u6bb5\uff0c\u5e76\u4e14\u5e0c\u671b\u7ec6\u80de\u7528\u5b83\u6765\u66ff\u6362\u6709\u7f3a\u9677\u7684\u5e8f\u5217\u3002<\/p>\n<p><br  \/><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\">\u7136\u800c\uff0c\u8fd9\u4e24\u4e2a\u8fc7\u7a0b\u6709\u70b9\u795e\u79d8\uff0c\u6ca1\u6709\u4eba\u77e5\u9053\u4e3a\u4ec0\u4e48\u4e00\u4e9b\u7ec6\u80de\u66f4\u5bb9\u6613\u63d2\u5165DNA\uff0c\u800c\u53e6\u4e00\u4e9b\u7ec6\u80de\u5374\u5f88\u5c11\u8fd9\u4e48\u505a\u3002<\/p>\n<p><br  \/><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\">\u201c\u5c06CRISPR-Cas9\u5e94\u7528\u4e8e\u533b\u5b66\u6216\u5408\u6210\u751f\u7269\u5b66\u5e94\u7528\u7684\u70ed\u60c5\u662f\u5de8\u5927\u7684\uff0c\u4f46\u6ca1\u6709\u4eba\u771f\u6b63\u77e5\u9053\u5b83\u690d\u5165\u7ec6\u80de\u540e\u4f1a\u53d1\u751f\u4ec0\u4e48\u3002\u201d\u6587\u7ae0\u4e00\u4f5c\u3001\u52a0\u5dde\u5927\u5b66\u4f2f\u514b\u5229\u5206\u6821\u535a\u58eb\u540eChris Richardson\u8868\u793a\uff0c\u201c\u5b83\u4f1a\u4ea7\u751fDNA\u65ad\u88c2\uff0c\u7136\u540e\u9700\u8981\u7ec6\u80de\u542f\u52a8\u4fee\u590d\u673a\u5236\u3002\u4f46\u662f\u6211\u4eec\u5e76\u4e0d\u4e86\u89e3\u8fd9\u80cc\u540e\u7684\u7ec6\u8282\u3002\u201d<\/p>\n<section class=\"\"  style=\"max-width: 100%;color: rgb(51, 51, 51);\">\n<section style=\"margin: 10px auto;max-width: 100%;text-align: center;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section style=\"margin-bottom: 3px;margin-left: 33px;max-width: 100%;font-size: 0px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"margin-bottom: 10px;max-width: 100%;width: 6px;height: 6px;display: inline-block;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><span style=\"max-width: 100%;display: inline-block;width: 10px;height: 10px;opacity: 0.6;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span>&nbsp;&nbsp;&nbsp;<\/span><\/section>\n<section style=\"margin-top: -2px;max-width: 100%;font-size: 0px;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;display: inline-block;width: 15px;height: 15px;border-width: 1px;border-style: solid;border-color: white;opacity: 0.9;vertical-align: bottom;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;display: inline-block;border-width: 1px;border-style: solid;border-color: rgb(255, 255, 255);width: 30px;height: 30px;line-height: 30px;vertical-align: top;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\">\n<p class=\"\" style=\"margin-right: auto;margin-left: auto;max-width: 100%;min-height: 1em;white-space: pre-wrap;min-width: 1px;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;\">02<\/p>\n<\/section>\n<section style=\"max-width: 100%;display: inline-block;width: 15px;height: 15px;border-width: 1px;border-style: solid;border-color: white;opacity: 0.9;vertical-align: top;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/section>\n<\/section>\n<section style=\"margin-top: 3px;margin-left: -33px;max-width: 100%;font-size: 0px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;display: inline-block;width: 10px;height: 10px;opacity: 0.6;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><span style=\"margin-bottom: -10px;max-width: 100%;width: 6px;height: 6px;display: inline-block;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span>&nbsp; &nbsp;<\/span><\/section>\n<\/section>\n<\/section>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);text-align: center;\"><span style=\"max-width: 100%;font-size: 16px;color: rgb(123, 12, 0);box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;text-align: justify;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">&nbsp;<span style=\"text-align: justify;\">\u89e3\u5f00\u8c1c\u56e2<\/span><\/span><\/span><\/strong><\/span><\/p>\n<p><br  \/><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\">\u4e3a\u4e86\u627e\u51fa\u54ea\u4e9bDNA\u4fee\u590d\u9176\u624d\u662fCRISPR\u526a\u5207\u4e4b\u540e\u540c\u6e90\u5b9a\u5411\u4fee\u590d\u7684\u5173\u952e\uff0cRichardson\u548cCorn\u91c7\u7528CRISPR\u5e72\u6270\uff08CRISPR interference\uff0cCRISPRi\uff09\u6280\u672f\u5728\u540c\u4e00\u65f6\u95f4\u6572\u9664\u8d852000\u4e2a\u5df2\u77e5\u6216\u8005\u6f5c\u5728\u7684\u4e0eDNA\u4fee\u590d\u6709\u5173\u7684\u57fa\u56e0\u3002<\/p>\n<p><br  \/><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\">\u4ee4\u4eba\u60ca\u8bb6\u7684\u662f\uff0c\u8bb8\u591a\u88ab\u8bc1\u660e\u5f88\u91cd\u8981\u7684\u57fa\u56e0\uff08\u4e00\u65e6\u6c89\u9ed8\uff0c\u540c\u6e90\u5b9a\u5411\u4fee\u590d\u4f1a\u6025\u5267\u4e0b\u964d\uff09\u4f3c\u4e4e\u4e0eCRISPR\u6ca1\u6709\u5173\u7cfb\u3002\u800c\u4e14\uff0c\u4ed6\u4eec\u53d1\u73b0\u4e86\u4e00\u6761\u7f55\u89c1\u7684\u57fa\u56e0\u4fee\u590d\u901a\u8def\uff0c\u5373\u8303\u53ef\u5c3c\u8d2b\u8840\u901a\u8def\uff0c\u8bc1\u5b9e\u5176\u5728CRISPR\u7f16\u8f91\u4e2d\u53d1\u6325\u91cd\u8981\u4f5c\u7528\u3002<\/p>\n<p><br  \/><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><strong>\u8303\u53ef\u5c3c\u8d2b\u8840\u662f\u4e00\u79cd\u7f55\u89c1\u4e14\u4e25\u91cd\u7684\u9057\u4f20\u6027\u75be\u75c5<\/strong>\u2014\u2014\u6e90\u4e8e\u4e0d\u80fd\u4ea7\u751f\u8db3\u591f\u591a\u7684\u65b0\u9c9c\u8840\u7ec6\u80de\uff0c\u4e34\u5e8a\u8868\u73b0\u4e3a\u591a\u6837\u5316\u5148\u5929\u7578\u5f62\u3001\u8fdb\u884c\u6027\u9aa8\u9ad3\u8870\u7aed\u3001\u8272\u7d20\u6c89\u7740\u75c7\u3001\u6076\u6027\u8840\u6db2\u7cfb\u7edf\u80bf\u7624\u53ca\u5b9e\u4f53\u7624\u503e\u5411\u3002\u5f88\u5c11\u6709\u75c5\u4eba\u6d3b\u523030\u5c81\u4ee5\u4e0a\u3002<\/p>\n<p><br  \/><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\">\u79d1\u5b66\u5bb6\u4eec\u5bf9\u8fd9\u4e00\u75c5\u7406\u7814\u7a76\u5df2\u8d8510\u5e74\uff0c\u53d1\u73b0\u8303\u53ef\u5c3c\u8d2b\u8840\u901a\u8def\u6d89\u53ca21\u79cd\u4e0d\u540c\u7684\u86cb\u767d\u8d28\uff0c\u4e00\u65e6\u4efb\u610f\u4e00\u4e2a\u86cb\u767d\u53d7\u635f\uff0c\u4eba\u4eec\u5c31\u4f1a\u60a3\u4e0a\u8303\u53ef\u5c3c\u8d2b\u8840\u3002\u6b64\u5916\uff0c\u8be5\u901a\u8def\u66f4\u503e\u5411\u4e8e\u4fee\u590d\u4e00\u4e2a\u7279\u5b9a\u7c7b\u578b\u7684DNA\u635f\u4f24\u2014\u2014DNA\u94fe\u95f4\u4ea4\u8054\uff08DNA interstrand crosslinks\uff09\uff0c\u53ef\u8f7b\u6613\u963b\u65adDNA\u590d\u5236\u3001\u8f6c\u5f55\u548c\u91cd\u7ec4\uff0c\u5c5e\u4e8e\u6bd2\u6027\u6700\u5f3a\u7684\u4e00\u79cdDNA\u635f\u4f24\uff0c\u53ef\u4ee5\u8f7b\u6613\u6740\u6b7b\u590d\u5236\u4e2d\u7684\u7ec6\u80de\u3002<\/p>\n<p><br  \/><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\">20\u4e16\u7eaa80\u5e74\u4ee3\uff0c\u7814\u7a76\u4eba\u5458\u66fe\u53d1\u73b0\uff0c\u540c\u6e90\u4ecb\u5bfc\u4fee\u590d\u4e0e\u8303\u53ef\u5c3c\u8d2b\u8840\u901a\u8def\u4e4b\u95f4\u5b58\u5728\u5173\u8054\uff0c\u4f46\u662f\u8fd9\u4e00\u7ebf\u7d22\u4e00\u76f4\u88ab\u5ffd\u7565\u3002<\/p>\n<p><br  \/><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\">\u201c\u6839\u636e\u6211\u4eec\u7684\u7814\u7a76\uff0c\u6211\u4eec\u8ba4\u4e3a\uff0c\u8303\u53ef\u5c3c\u8d2b\u8840\u901a\u8def\u5728\u4fee\u590d\u5176\u4ed6\u7c7b\u578b\u7684\u75c5\u53d8\u4e2d\u540c\u6837\u53d1\u6325\u7740\u91cd\u8981\u7684\u4f5c\u7528\uff0c\u4f46\u662f\u6700\u7cbe\u5f69\u7684\u662f\u4fee\u590d\u53cc\u94fe\u65ad\u88c2\u3002\u201d Richardson\u8868\u793a\uff0c\u201c<strong>\u5728Cas9\u7f16\u8f91\u4e4b\u540e\uff0c\u8303\u53ef\u5c3c\u8d2b\u8840\u901a\u8def\u53ef\u4ee5\u63d2\u5165\u65b0\u7684DNA\u3002\u201d<\/strong><\/p>\n<section class=\"\"  style=\"max-width: 100%;color: rgb(51, 51, 51);\">\n<section style=\"margin: 10px auto;max-width: 100%;text-align: center;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section style=\"margin-bottom: 3px;margin-left: 33px;max-width: 100%;font-size: 0px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"margin-bottom: 10px;max-width: 100%;width: 6px;height: 6px;display: inline-block;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><span style=\"max-width: 100%;display: inline-block;width: 10px;height: 10px;opacity: 0.6;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span>&nbsp;&nbsp;&nbsp;<\/span><\/section>\n<section style=\"margin-top: -2px;max-width: 100%;font-size: 0px;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;display: inline-block;width: 15px;height: 15px;border-width: 1px;border-style: solid;border-color: white;opacity: 0.9;vertical-align: bottom;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;display: inline-block;border-width: 1px;border-style: solid;border-color: rgb(255, 255, 255);width: 30px;height: 30px;line-height: 30px;vertical-align: top;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\">\n<p class=\"\" style=\"margin-right: auto;margin-left: auto;max-width: 100%;min-height: 1em;white-space: pre-wrap;min-width: 1px;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;\">03<\/p>\n<\/section>\n<section style=\"max-width: 100%;display: inline-block;width: 15px;height: 15px;border-width: 1px;border-style: solid;border-color: white;opacity: 0.9;vertical-align: top;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/section>\n<\/section>\n<section style=\"margin-top: 3px;margin-left: -33px;max-width: 100%;font-size: 0px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;display: inline-block;width: 10px;height: 10px;opacity: 0.6;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><span style=\"margin-bottom: -10px;max-width: 100%;width: 6px;height: 6px;display: inline-block;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span>&nbsp; &nbsp;<\/span><\/section>\n<\/section>\n<\/section>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);text-align: center;\"><span style=\"max-width: 100%;font-size: 16px;color: rgb(123, 12, 0);box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;text-align: justify;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"text-align: justify;\">\u98a0\u8986\u65e7\u8bc6<\/span><\/span><\/span><\/strong><\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><br  \/><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\">\u8fd9\u4e00\u53d1\u73b0\u5f15\u53d1\u4e86\u65b0\u7684\u8d28\u7591\uff1a\u5982\u679c\u8303\u53ef\u5c3c\u8d2b\u8840\u901a\u8def\u672a\u66fe\u6fc0\u6d3b\uff0c\u7ec6\u80de\u53ef\u80fd\u65e0\u6cd5\u5728\u57fa\u56e0\u7f16\u8f91\u4e4b\u540e\u7528\u6b63\u5e38\u57fa\u56e0\u66ff\u6362\u7a81\u53d8\u7684\u57fa\u56e0\u3002\u4e8b\u5b9e\u4e0a\uff0c\u8303\u53ef\u5c3c\u8d2b\u8840\u901a\u8def\u7684\u6d3b\u6027\u6c34\u5e73\u53ef\u80fd\u4f1a\u5f71\u54cdCRISPR\u7cbe\u786e\u5728\u7279\u5b9a\u7ec6\u80de\u4e2d\u63d2\u5165DNA\u7684\u6548\u7387\u3002<\/p>\n<p><br  \/><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\">\u7814\u7a76\u4eba\u5458\u5f97\u51fa\u7ed3\u8bba\uff1a<strong>\u867d\u7136\u975e\u540c\u6e90\u6027\u672b\u7aef\u8fde\u63a5\u662f\u53cc\u94fe\u65ad\u88c2\u540e\u7684\u9ed8\u8ba4\u4fee\u590d\u673a\u5236\uff0c\u4f46\u662f\u8303\u53ef\u5c3c\u8d2b\u8840\u901a\u8def\u4f1a\u4e0e\u4e4b\u7ade\u4e89\uff0c\u6d3b\u6027\u8d8a\u9ad8\u4f1a\u5bfc\u81f4\u66f4\u591a\u7684\u540c\u6e90\u4ecb\u5bfc\u4fee\u590d\u3001\u66f4\u5c11\u7684\u7aef\u8fde\u63a5\u3002<\/strong><\/p>\n<section class=\"\"  style=\"max-width: 100%;color: rgb(51, 51, 51);\">\n<section style=\"margin: 10px auto;max-width: 100%;text-align: center;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section style=\"margin-bottom: 3px;margin-left: 33px;max-width: 100%;font-size: 0px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"margin-bottom: 10px;max-width: 100%;width: 6px;height: 6px;display: inline-block;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><span style=\"max-width: 100%;display: inline-block;width: 10px;height: 10px;opacity: 0.6;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span>&nbsp;&nbsp;&nbsp;<\/span><\/section>\n<section style=\"margin-top: -2px;max-width: 100%;font-size: 0px;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;display: inline-block;width: 15px;height: 15px;border-width: 1px;border-style: solid;border-color: white;opacity: 0.9;vertical-align: bottom;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/section>\n<section style=\"max-width: 100%;display: inline-block;border-width: 1px;border-style: solid;border-color: rgb(255, 255, 255);width: 30px;height: 30px;line-height: 30px;vertical-align: top;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\">\n<p class=\"\" style=\"margin-right: auto;margin-left: auto;max-width: 100%;min-height: 1em;white-space: pre-wrap;min-width: 1px;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;\">04<\/p>\n<\/section>\n<section style=\"max-width: 100%;display: inline-block;width: 15px;height: 15px;border-width: 1px;border-style: solid;border-color: white;opacity: 0.9;vertical-align: top;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/section>\n<\/section>\n<section style=\"margin-top: 3px;margin-left: -33px;max-width: 100%;font-size: 0px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;display: inline-block;width: 10px;height: 10px;opacity: 0.6;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span><span style=\"margin-bottom: -10px;max-width: 100%;width: 6px;height: 6px;display: inline-block;color: rgb(255, 255, 255);background-color: rgb(172, 29, 16);box-sizing: border-box !important;word-wrap: break-word !important;\"><\/span>&nbsp; &nbsp;<\/span><\/section>\n<\/section>\n<\/section>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);text-align: center;\"><span style=\"max-width: 100%;font-size: 16px;color: rgb(123, 12, 0);box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;text-align: justify;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"text-align: justify;\">\u7814\u7a76\u610f\u4e49<\/span>&nbsp;<\/span><\/span><\/strong><\/span><\/p>\n<p><br  \/><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\">\u8fd9\u4e9b\u53d1\u73b0\u4e0d\u4ec5\u6709\u52a9\u4e8e\u79d1\u5b66\u5bb6\u4eec\u66f4\u597d\u5730\u4e86\u89e3\u7ec6\u80de\u7684DNA\u4fee\u590d\u673a\u5236\uff0c\u8fd8\u53ef\u4ee5\u5e2e\u52a9\u4ed6\u4eec\u5f00\u53d1\u9488\u5bf9\u764c\u7ec6\u80deDNA\u4fee\u590d\u7684\u6297\u764c\u7597\u6cd5\u3002<\/p>\n<p><br  \/><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\">Richardson\u53d1\u73b0\uff0c\u5173\u8054\u8303\u53ef\u5c3c\u8d2b\u8840\u901a\u8def\u768421\u79cd\u86cb\u767d\u8d28\u4e2d\u7684FANCD2\u86cb\u767d\u603b\u662f\u4f4d\u4e8eCRISPR-Cas9\u521b\u5efa\u7684\u53cc\u94fe\u65ad\u88c2\u7684\u4f4d\u7f6e\u3002\u8fd9\u610f\u5473\u7740\uff0cFANCD2\u5728\u8c03\u63a7\u65b0DNA\u63d2\u5165\u57fa\u56e0\u7ec4\u526a\u5207\u4f4d\u70b9\u4e2d\u53d1\u6325\u7740\u91cd\u8981\u4f5c\u7528\u3002\u79d1\u5b66\u5bb6\u4eec\u63a8\u6d4b\uff0cFANCD2\u53ef\u4ee5\u88ab\u5fae\u8c03\uff0c\u4ece\u800c\u63d0\u9ad8\u7ec6\u80de\u901a\u8fc7\u540c\u6e90\u5b9a\u5411\u4fee\u590d\u63d2\u5165DNA\u7684\u9891\u7387\u3002<\/p>\n<p><br  \/><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\">\u201c\u6b64\u5916\uff0c\u8003\u8651\u5230FANCD2\u4f4d\u4e8eCas9\u526a\u5207\u7684\u4f4d\u7f6e\uff0c\u6211\u4eec\u6216\u8bb8\u53ef\u4ee5\u5229\u7528FANCD2\u6765\u7ed8\u5236Cas9\u5728\u4efb\u4f55\u7ec6\u80de\u7c7b\u578b\u4e2d\u5207\u5272\u7684\u4f4d\u7f6e\u3002\u201d Richardson\u8bbe\u60f3\u9053\u3002<\/p>\n<p><br  \/><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\">\u79d1\u5b66\u5bb6\u4eec\u53ef\u4ee5\u5bf9\u53c2\u4e0e\u8fd9\u4e00\u901a\u8def\u7684\u86cb\u767d\u8d28\u8fdb\u884c\u201c\u4eba\u4e3a\u8c03\u6574\u201d\uff0c\u4f18\u5148\u5f15\u5bfcDNA\u7f6e\u6362\u7ed3\u679c\uff0c\u8fd9\u5bf9\u4e8e\u57fa\u56e0\u6cbb\u7597\u9057\u4f20\u75be\u75c5\u5f88\u91cd\u8981\u3002<\/p>\n<p><br  \/><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\">\u201c\u5982\u679c\u4f60\u60f3\u8981\u6cbb\u7597\u9570\u72b6\u7ec6\u80de\u8d2b\u8840\uff0c\u6210\u529f\u7387\u4e0e\u4f60\u4f7f\u7528\u6b63\u786e\u7684\u57fa\u56e0\u66ff\u6362\u7f3a\u9677\u57fa\u56e0\u7684\u6548\u7387\u5bc6\u4e0d\u53ef\u5206\u3002\u201dRichardson\u89e3\u91ca\u8bf4\uff0c\u201c\u5982\u679c\u4f60\u4ece\u75c5\u4eba\u8eab\u4e0a\u91c7\u96c6\u4e00\u767e\u4e07\u4e2a\u7ec6\u80de\uff0c\u6700\u7ec8\u83b7\u5f9710%\u7684\u63d2\u5165\u7387\uff0c\u81ea\u7136\u6ca1\u670930-40%\u90a3\u4e48\u597d\u3002\u201d<\/p>\n<p style=\"line-height: 1.75em;\"><br  \/><\/p>\n<p style=\"line-height: 1.75em;text-align: right;\"><strong>\u8d23\u7f16\uff1a\u98ce\u94c3<\/strong><\/p>\n<p style=\"line-height: 1.75em;text-align: right;\"><strong><br  \/><\/strong><\/p>\n<\/p>\n<p><br  \/><\/p>\n<p style=\"line-height: 1.75em;text-align: left;\"><strong style=\"max-width: 100%;color: rgb(51, 51, 51);\">\u5907\u6ce8\uff1a<\/strong><span style=\"color: rgb(51, 51, 51);\">\u70b9\u51fb\u9605\u8bfb\u539f\u6587\u67e5\u770b\u201c\u4e09\u7ef4\u57fa\u56e0\u7ec4\u4e0e\u4eba\u9020\u751f\u547d\u7684\u5947\u8ff9\u2014\u2014Nature\u8bba\u6587\u5206\u4eab\u4f1a\u201d\u8be6\u60c5\u3002<\/span><\/p>\n<section  class=\"\" style=\"max-width: 100%;color: rgb(51, 51, 51);\">\n<section  style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section class=\"\" style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section  class=\"\" style=\"max-width: 100%;line-height: 28.4444px;font-family: \u5fae\u8f6f\u96c5\u9ed1;border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box !important;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<section  style=\"margin: 1em auto;max-width: 100%;border-width: 0px;border-style: none;border-color: initial;text-align: center;width: 941px;box-sizing: border-box !important;word-wrap: break-word !important;overflow-wrap: break-word !important;\"><span class=\"\"  style=\"max-width: 100%;width: 941px;border-bottom: 1px solid rgb(172, 29, 16);border-top-color: rgb(172, 29, 16);border-right-color: rgb(172, 29, 16);border-left-color: rgb(172, 29, 16);display: inline-block;box-sizing: border-box !important;word-wrap: break-word !important;overflow-wrap: break-word !important;\"><\/p>\n<section class=\"\" style=\"margin-bottom: -3px;padding-top: 5px;padding-right: 10px;padding-left: 10px;max-width: 100%;display: inline-block;min-height: 32px;border-bottom: 5px solid rgb(172, 29, 16);font-size: 20px;color: rgb(121, 121, 121);box-sizing: border-box !important;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<p class=\"\" style=\"margin-top: 5px;margin-bottom: 5px;max-width: 100%;min-height: 1em;line-height: 1.6em;font-size: 16px;box-sizing: border-box !important;word-wrap: break-word !important;overflow-wrap: break-word !important;\">End<\/p>\n<\/section>\n<p><\/span><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p class=\"\" style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><strong class=\"\" style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><span class=\"\" style=\"max-width: 100%;font-size: 12px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u53c2\u8003\u8d44\u6599\uff1a<\/span><\/strong><span style=\"max-width: 100%;font-size: 12px;box-sizing: border-box !important;word-wrap: break-word !important;\">1\uff09<span style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">DNA repair after CRISPR cutting not at all what people thought<\/span><\/span><\/p>\n<p class=\"\" style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<section  class=\"\" style=\"max-width: 100%;box-sizing: border-box;letter-spacing: 0.544px;text-align: justify;white-space: normal;background-color: rgb(255, 255, 255);font-size: 16px;color: rgb(62, 62, 62);line-height: 25.6px;font-family: \u5fae\u8f6f\u96c5\u9ed1;border-width: 0px;border-style: none;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<section class=\"\"  style=\"padding: 2px;max-width: 100%;box-sizing: border-box;border-width: 1px;border-style: dotted;border-color: rgb(218, 98, 71);word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<section class=\"\" style=\"margin: 5px auto;max-width: 100%;box-sizing: border-box;border-width: 0px;border-style: none;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<section class=\"\" style=\"max-width: 100%;box-sizing: border-box;height: 25px;color: inherit;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<section class=\"\" style=\"max-width: 100%;box-sizing: border-box;height: 25px;width: 50px;float: left;border-top: 2px solid rgb(172, 29, 16);border-right-color: rgb(172, 29, 16);border-bottom-color: rgb(172, 29, 16);border-left: 2px solid rgb(172, 29, 16);color: inherit;word-wrap: break-word !important;overflow-wrap: break-word !important;\"><\/section>\n<section class=\"\" style=\"max-width: 100%;box-sizing: border-box;display: inline-block;color: rgb(172, 29, 16);clear: both;border-color: rgb(172, 29, 16);word-wrap: break-word !important;overflow-wrap: break-word !important;\"><\/section>\n<\/section>\n<section class=\"\"  style=\"margin: -0.8em 0.3em;padding: 0.8em;max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;color: inherit;overflow-wrap: break-word !important;\">\n<p class=\"\" style=\"max-width: 100%;min-height: 1em;overflow-wrap: break-word;color: rgb(51, 51, 51);font-size: 1em;line-height: 1.75em;word-break: break-all;box-sizing: border-box !important;word-wrap: break-word !important;\"><span class=\"\" style=\"max-width: 100%;color: rgb(0, 0, 0);line-height: 22.4px;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\u672c\u6587\u7cfb\u751f\u7269\u63a2\u7d22\u539f\u521b\uff0c\u6b22\u8fce\u4e2a\u4eba\u8f6c\u53d1\u5206\u4eab\u3002\u5176\u4ed6\u4efb\u4f55\u5a92\u4f53\u3001\u7f51\u7ad9\u5982\u9700\u8f6c\u8f7d\uff0c\u987b\u5728\u6b63\u6587\u524d\u6ce8\u660e\u6765\u6e90\u751f\u7269\u63a2\u7d22\u3002<\/span><\/p>\n<\/section>\n<section class=\"\" style=\"max-width: 100%;box-sizing: border-box;height: 25px;color: inherit;word-wrap: break-word !important;overflow-wrap: break-word !important;\">\n<section class=\"\" style=\"max-width: 100%;box-sizing: border-box;height: 25px;width: 50px;float: right;border-bottom: 2px solid rgb(172, 29, 16);border-top-color: rgb(172, 29, 16);border-right: 2px solid rgb(172, 29, 16);border-left-color: rgb(172, 29, 16);color: inherit;word-wrap: break-word !important;overflow-wrap: break-word !important;\"><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p class=\"\" style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><img class=\"\"       style=\"box-sizing: border-box !important;word-wrap: break-word !important;visibility: visible !important;width: auto !important;\" width=\"auto\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/08\/beepress0-1533788190.png\" alt=\"\u201c\u9b54\u526a\u201d\u526a\u5207\u540e\uff0c\u7ec6\u80de\u5982\u4f55\u4fee\u590d\uff1fNature\u5b50\u520a\uff1a\u6ca1\u90a3\u4e48\u7b80\u5355\" title=\"\u201c\u9b54\u526a\u201d\u526a\u5207\u540e\uff0c\u7ec6\u80de\u5982\u4f55\u4fee\u590d\uff1fNature\u5b50\u520a\uff1a\u6ca1\u90a3\u4e48\u7b80\u5355\"  \/><\/p>\n<p><br  \/><\/p>\n<blockquote class='keep-source'>\n<p>\u59cb\u53d1\u4e8e\u5fae\u4fe1\u516c\u4f17\u53f7\uff1a                         \u751f\u7269\u63a2\u7d22                      <\/p>\n<\/blockquote>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u751f\u7269\u63a2\u7d22 \u7f16\u8005\u6309 CRISPR\u81ea\u95ee\u4e16\u4ee5\u6765\u4e00\u76f4\u88ab\u5bc4\u4e88\u539a\u671b\uff0c\u4f46\u662f\u5bf9\u4e8e\u5b83\u5728\u7ec6\u80de\u4e2d\u7684\u4f5c\u7528\u7ec6\u8282\uff0c\u4f9d\u7136\u6709\u5f88\u591a\u672a\u77e5\u3002\u8fd1\u65e5\uff0c\u79d1 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":31469,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[2],"tags":[],"_links":{"self":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/31468"}],"collection":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/comments?post=31468"}],"version-history":[{"count":1,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/31468\/revisions"}],"predecessor-version":[{"id":31478,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/31468\/revisions\/31478"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media\/31469"}],"wp:attachment":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media?parent=31468"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/categories?post=31468"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/tags?post=31468"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}