{"id":35149,"date":"2018-09-05T01:00:00","date_gmt":"2018-09-17T05:50:29","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/2018\/09\/17\/%e6%b5%81%e5%8a%a8%e7%9b%b8%e9%85%8d%e7%bd%ae%e8%bf%87%e7%a8%8b%e4%b8%ad%e5%ba%94%e8%af%a5%e9%81%bf%e5%85%8d%e7%9a%8410%e4%b8%aa%e5%9d%8f%e4%b9%a0%e6%83%af%ef%bc%8c%e4%bd%a0%e4%b8%ad%e6%8b%9b%e4%ba%86\/"},"modified":"2018-09-17T13:50:33","modified_gmt":"2018-09-17T05:50:33","slug":"%e6%b5%81%e5%8a%a8%e7%9b%b8%e9%85%8d%e7%bd%ae%e8%bf%87%e7%a8%8b%e4%b8%ad%e5%ba%94%e8%af%a5%e9%81%bf%e5%85%8d%e7%9a%8410%e4%b8%aa%e5%9d%8f%e4%b9%a0%e6%83%af%ef%bc%8c%e4%bd%a0%e4%b8%ad%e6%8b%9b%e4%ba%86","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/35149\/","title":{"rendered":"\u6d41\u52a8\u76f8\u914d\u7f6e\u8fc7\u7a0b\u4e2d\u5e94\u8be5\u907f\u514d\u768410\u4e2a\u574f\u4e60\u60ef\uff0c\u4f60\u4e2d\u62db\u4e86\u6ca1\uff1f"},"content":{"rendered":"
\n


<\/p>\n

\n
\n

\u70b9\u51fb\u84dd\u8272\u5b57\u5173\u6ce8\u6211\u4eec\uff01
<\/p>\n

\"\u6d41\u52a8\u76f8\u914d\u7f6e\u8fc7\u7a0b\u4e2d\u5e94\u8be5\u907f\u514d\u768410\u4e2a\u574f\u4e60\u60ef\uff0c\u4f60\u4e2d\u62db\u4e86\u6ca1\uff1f\"<\/section>\n<\/section>\n

\"\u6d41\u52a8\u76f8\u914d\u7f6e\u8fc7\u7a0b\u4e2d\u5e94\u8be5\u907f\u514d\u768410\u4e2a\u574f\u4e60\u60ef\uff0c\u4f60\u4e2d\u62db\u4e86\u6ca1\uff1f\"<\/p>\n

\u672c\u6587\u8f6c\u81ea\u201c\u836f\u7269\u5206\u6790\u4e4b\u5bb6\u201d\uff0c\u6b22\u8fce\u5173\u6ce8\uff01<\/span>
<\/p>\n

HPLC Mobile Phases \u2013 10 bad habits to avoid<\/span><\/strong><\/p>\n

\u6d41\u52a8\u76f8\u914d\u7f6e\u8fc7\u7a0b\u4e2d\u5e94\u8be5\u907f\u514d\u768410\u4e2a\u574f\u4e60\u60ef<\/span><\/strong><\/p>\n


<\/span><\/p>\n

\"\u6d41\u52a8\u76f8\u914d\u7f6e\u8fc7\u7a0b\u4e2d\u5e94\u8be5\u907f\u514d\u768410\u4e2a\u574f\u4e60\u60ef\uff0c\u4f60\u4e2d\u62db\u4e86\u6ca1\uff1f\"<\/p>\n

1. Measuring the pH of the mobile phase after the organic has been added<\/span><\/strong><\/p>\n

\u5728\u6dfb\u52a0\u6709\u673a\u76f8\u540e\u6d4b\u91cf\u6d41\u52a8\u76f8\u7684pH\u503c<\/span><\/strong><\/p>\n

\"\u6d41\u52a8\u76f8\u914d\u7f6e\u8fc7\u7a0b\u4e2d\u5e94\u8be5\u907f\u514d\u768410\u4e2a\u574f\u4e60\u60ef\uff0c\u4f60\u4e2d\u62db\u4e86\u6ca1\uff1f\"<\/span><\/strong><\/p>\n

pH meters are calibrated to give the correctpH readback in aqueous solution \u2013 the buffers you verify this with are aqueous.If you measure the pH with the organic added, the pH will be different to thatof measuring before organic addition.<\/span><\/p>\n

\u5728\u6c34\u6eb6\u6db2\u4e2d\uff0cpH\u503c\u88ab\u6821\u51c6\u4ee5\u63d0\u4f9b\u6b63\u786e\u7684pH\u503c\u2014\u2014\u4f60\u9700\u8981\u786e\u8ba4\u7684\u7f13\u51b2\u6db2\u662f\u6c34\u6eb6\u6db2\u3002\u5982\u679c\u4f60\u7528\u6dfb\u52a0\u4e86\u6709\u673a\u76f8\u7684\u6c34\u6eb6\u6db2\u6765\u6d4b\u91cfpH\u503c\uff0cpH\u503c\u5c31\u4f1a\u4e0e\u6709\u673a\u76f8\u6dfb\u52a0\u524d\u7684pH\u503c\u4e0d\u540c\u3002<\/span><\/p>\n

However, the most important point is to beconsistent.If you do always measure pH after theorganic is added, make sure you state this in the method so that everyone doesit the same way. It won\u2019t be 100% accurate, but at least it will be consistent.This is probably more important than having the exact pH.<\/span><\/p>\n

\u7136\u800c\uff0c\u6700\u91cd\u8981\u7684\u4e00\u70b9\u662f\u8981\u4fdd\u6301\u4e00\u81f4\u3002\u5982\u679c\u4f60\u603b\u662f\u5728\u6dfb\u52a0\u6709\u673a\u76f8\u540e\u6d4b\u91cfpH\u503c\uff0c\u786e\u4fdd\u4f60\u5728\u65b9\u6cd5\u4e2d\u8bf4\u660e\u4e86\u8fd9\u4e00\u70b9\uff0c\u4ee5\u4fbf\u6bcf\u4e2a\u4eba\u90fd\u7528\u540c\u6837\u7684\u65b9\u6cd5\u3002\u5b83\u4e0d\u4f1a\u767e\u5206\u767e\u51c6\u786e\uff0c\u4f46\u81f3\u5c11\u4f1a\u662f\u4e00\u81f4\u7684\u3002\u8fd9\u53ef\u80fd\u6bd4\u7cbe\u786e\u7684pH\u503c\u66f4\u91cd\u8981\u3002<\/span><\/p>\n

\"\u6d41\u52a8\u76f8\u914d\u7f6e\u8fc7\u7a0b\u4e2d\u5e94\u8be5\u907f\u514d\u768410\u4e2a\u574f\u4e60\u60ef\uff0c\u4f60\u4e2d\u62db\u4e86\u6ca1\uff1f\"<\/p>\n

2. Not using a buffer<\/span><\/strong><\/p>\n

\u6ca1\u6709\u4f7f\u7528\u7f13\u51b2\u6db2<\/span><\/strong><\/p>\n

\"\u6d41\u52a8\u76f8\u914d\u7f6e\u8fc7\u7a0b\u4e2d\u5e94\u8be5\u907f\u514d\u768410\u4e2a\u574f\u4e60\u60ef\uff0c\u4f60\u4e2d\u62db\u4e86\u6ca1\uff1f\"<\/span><\/strong><\/p>\n

Buffers are present to control pH and resist a change in pH.Many other parts of method (e.g. sample matrix, CO2inair, source of water used for your mobile phase) can change the pH of themobile phase causing shifts in retention, peak shape and peak response.Formicacid, TFA etc. are not buffers.<\/span><\/p>\n

\u7f13\u51b2\u6db2\u662f\u7528\u6765\u63a7\u5236pH\u503c\u548c\u62b5\u6297pH\u503c\u53d8\u5316\u7684\u3002\u65b9\u6cd5\u7684\u8bb8\u591a\u5176\u4ed6\u90e8\u5206(\u4f8b\u5982\u6837\u672c\u77e9\u9635\u3001\u7a7a\u6c14\u4e2d\u7684\u4e8c\u6c27\u5316\u78b3\u3001\u6d41\u52a8\u76f8\u4e2d\u6c34\u7684\u6765\u6e90)\u53ef\u4ee5\u6539\u53d8\u6d41\u52a8\u76f8\u7684pH\u503c\uff0c\u4ece\u800c\u5bfc\u81f4\u4fdd\u7559\u3001\u5cf0\u5f62\u548c\u5cf0\u503c\u54cd\u5e94\u7684\u53d8\u5316\u3002\u7532\u9178\uff0c\u4e09\u6c1f\u4e59\u9178\u7b49\u4e0d\u5c5e\u4e8e\u7f13\u51b2\u8bd5\u5242\u3002<\/span><\/p>\n

\"\u6d41\u52a8\u76f8\u914d\u7f6e\u8fc7\u7a0b\u4e2d\u5e94\u8be5\u907f\u514d\u768410\u4e2a\u574f\u4e60\u60ef\uff0c\u4f60\u4e2d\u62db\u4e86\u6ca1\uff1f\"<\/p>\n

3. Not using the buffer in its correct pH range<\/span><\/strong><\/p>\n

\u6ca1\u6709\u5728\u7f13\u51b2\u6db2\u6b63\u786e\u7684pH\u8303\u56f4\u5185\u4f7f\u7528<\/span><\/strong><\/p>\n

\"\u6d41\u52a8\u76f8\u914d\u7f6e\u8fc7\u7a0b\u4e2d\u5e94\u8be5\u907f\u514d\u768410\u4e2a\u574f\u4e60\u60ef\uff0c\u4f60\u4e2d\u62db\u4e86\u6ca1\uff1f\"<\/span><\/strong><\/p>\n

Each buffer salt has a 2 pH unit wide rangeover which it provides the optimal pH stability. Outside this window the saltis ineffective at resisting change in pH.Either use your buffer within thecorrect range or pick a buffer whose range covers the pH you require.<\/span><\/p>\n

\u6bcf\u4e2a\u7f13\u51b2\u76d0\u67092\u4e2apH\u503c\u5355\u4f4d\u8303\u56f4\uff0c\u5b83\u63d0\u4f9b\u4e86\u6700\u4f73\u7684pH\u7a33\u5b9a\u6027\u3002\u5728\u7f13\u51b2\u8303\u56f4\u5916\u9762\uff0c\u76d0\u5bf9pH\u503c\u7684\u53d8\u5316\u662f\u65e0\u6548\u7684\u3002\u8981\u4e48\u5728\u6b63\u786e\u7684\u8303\u56f4\u5185\u4f7f\u7528\u7f13\u51b2\u533a\uff0c\u8981\u4e48\u9009\u62e9\u4e00\u4e2a\u8303\u56f4\u8986\u76d6\u6240\u9700pH\u503c\u7684\u7f13\u51b2\u533a\u3002<\/span><\/p>\n

\"\u6d41\u52a8\u76f8\u914d\u7f6e\u8fc7\u7a0b\u4e2d\u5e94\u8be5\u907f\u514d\u768410\u4e2a\u574f\u4e60\u60ef\uff0c\u4f60\u4e2d\u62db\u4e86\u6ca1\uff1f\"<\/p>\n

4. Adding buffer to organic<\/strong><\/span><\/p>\n

\u5c06\u7f13\u51b2\u6db2\u52a0\u5165\u6709\u673a\u76f8\u4e2d<\/strong><\/span><\/p>\n

\"\u6d41\u52a8\u76f8\u914d\u7f6e\u8fc7\u7a0b\u4e2d\u5e94\u8be5\u907f\u514d\u768410\u4e2a\u574f\u4e60\u60ef\uff0c\u4f60\u4e2d\u62db\u4e86\u6ca1\uff1f\"<\/strong><\/span><\/p>\n

Mixing aqueous buffer into the organic phasecarries a high risk of the buffer being precipitated \u2013 in many cases so finely that it may notbe obvious it has happened.Alwaysadd the organic to the aqueous phase, this greatly reducesthe risk of buffer precipitation.<\/span><\/p>\n

\u5c06\u6c34\u7684\u7f13\u51b2\u6db2\u6df7\u5408\u5230\u6709\u673a\u76f8\u4e2d\uff0c\u4f1a\u5bfc\u81f4\u7f13\u51b2\u6db2\u6c89\u6dc0\u7684\u9ad8\u98ce\u9669\u2014\u2014\u5728\u8bb8\u591a\u60c5\u51b5\u4e0b\uff0c\u53d8\u5316\u5fae\u5c0f\uff0c\u4ee5\u81f3\u4e8e\u53ef\u80fd\u4e0d\u592a\u660e\u663e\u3002\u5e38\u5e38\u662f\u5c06\u6709\u673a\u7269\u8d28\u6dfb\u52a0\u5230\u6c34\u76f8\uff0c\u8fd9\u5927\u5927\u964d\u4f4e\u4e86\u7f13\u51b2\u6c89\u6dc0\u7684\u98ce\u9669\u3002<\/span><\/p>\n

\"\u6d41\u52a8\u76f8\u914d\u7f6e\u8fc7\u7a0b\u4e2d\u5e94\u8be5\u907f\u514d\u768410\u4e2a\u574f\u4e60\u60ef\uff0c\u4f60\u4e2d\u62db\u4e86\u6ca1\uff1f\"<\/p>\n

5. Using the pump to mix gradients from 0%<\/span><\/strong><\/p>\n

\u4f7f\u7528\u6cf5\u7684\u68af\u5ea6\u6df7\u5408\u4ece0%<\/span><\/strong><\/p>\n

\"\u6d41\u52a8\u76f8\u914d\u7f6e\u8fc7\u7a0b\u4e2d\u5e94\u8be5\u907f\u514d\u768410\u4e2a\u574f\u4e60\u60ef\uff0c\u4f60\u4e2d\u62db\u4e86\u6ca1\uff1f\"<\/span><\/strong><\/p>\n

Modern pumps are very effective at mixingmobile phases and degassing online, however not everyone who ends up using yourmethod has a high quality pump. Premix your A and B starting mix to a singlesolution that runs at 100% on line A. e.g.<\/span><\/p>\n

\u73b0\u4ee3\u6cf5\u5728\u6df7\u5408\u6d41\u52a8\u76f8\u548c\u5728\u7ebf\u8131\u6c14\u4e0a\u975e\u5e38\u6709\u6548\uff0c\u7136\u800c\uff0c\u5e76\u4e0d\u662f\u6bcf\u4e2a\u4f7f\u7528\u4f60\u7684\u65b9\u6cd5\u7684\u4eba\u90fd\u6709\u4e00\u4e2a\u9ad8\u8d28\u91cf\u7684\u6cf5\u3002\u9884\u6df7\u5408\u4f60\u7684A\u548cB\uff0c\u6df7\u5408\u6210\u4e00\u4e2a\u5355\u4e00\u7684\u6eb6\u6db2\uff0c\u5728A\u7ba1\u8def\u4e0a100%\u8fd0\u884c\u3002<\/span><\/p>\n

Prepare the starting mixture by mixing 950mlAqueous with 50ml organic, then filter and degas. This reduces variabilitybetween HPLCs, reduces the risk of bubbles and precipitation in the system.<\/span><\/p>\n

\u5c06950mL\u7684\u6c34\u6eb6\u6db2\u4e0e50mL\u7684\u6709\u673a\u6eb6\u6db2\u6df7\u5408\uff0c\u7136\u540e\u8fc7\u6ee4\u548c\u8131\u6c14\u3002\u8fd9\u964d\u4f4e\u4e86HPLC\u4e4b\u95f4\u7684\u53ef\u53d8\u6027\uff0c\u964d\u4f4e\u4e86\u7cfb\u7edf\u4e2d\u6c14\u6ce1\u548c\u6c89\u964d\u7684\u98ce\u9669\u3002<\/span><\/p>\n

Note however that 95:5 mixed on the pumpwill not give the same retention time as 95:5 premixed in the bottle \u2013 younormally need to add a few more percent organic when premixing.<\/span><\/p>\n

\u6ce8\u610f\uff0c\u5728\u6cf5\u4e0a\u6df7\u5408\u768495:5\u4e0d\u4f1a\u548c\u5728\u74f6\u4e2d\u9884\u62cc\u768495:5\u76f8\u540c\u7684\u4fdd\u7559\u65f6\u95f4\u2014\u2014\u4f60\u901a\u5e38\u9700\u8981\u5728\u9884\u6df7\u5408\u65f6\u6dfb\u52a0\u66f4\u591a\u7684\u6709\u673a\u6210\u5206\u3002<\/span><\/p>\n

\"\u6d41\u52a8\u76f8\u914d\u7f6e\u8fc7\u7a0b\u4e2d\u5e94\u8be5\u907f\u514d\u768410\u4e2a\u574f\u4e60\u60ef\uff0c\u4f60\u4e2d\u62db\u4e86\u6ca1\uff1f\"<\/p>\n

6. Not using the correct pH modifying acid or base for your buffer<\/span><\/strong><\/p>\n

\u6ca1\u6709\u4f7f\u7528\u6b63\u786e\u7684\u9178\u6216\u78b1\u8c03\u8282\u4f60\u7684\u7f13\u51b2\u6db2\u7684pH\u503c<\/span><\/strong><\/p>\n

\"\u6d41\u52a8\u76f8\u914d\u7f6e\u8fc7\u7a0b\u4e2d\u5e94\u8be5\u907f\u514d\u768410\u4e2a\u574f\u4e60\u60ef\uff0c\u4f60\u4e2d\u62db\u4e86\u6ca1\uff1f\"<\/span><\/strong><\/p>\n

Only use the acid or base that forms thebuffer salt you are using. E.g. sodium phosphate buffers should be adjustedwith only phosphoric acid or sodium hydroxide.<\/span><\/p>\n

\u53ea\u4f7f\u7528\u4f60\u6b63\u5728\u4f7f\u7528\u7684\u7f13\u51b2\u76d0\u7684\u9178\u6216\u78b1\u3002\u4e3e\u4f8b\u6765\u8bf4\uff0c\u78f7\u9178\u94a0\u7f13\u51b2\u6db2\u5e94\u8be5\u4ec5\u7528\u78f7\u9178\u6216\u6c22\u6c27\u5316\u94a0\u6765\u8c03\u8282\u3002<\/span><\/p>\n

\"\u6d41\u52a8\u76f8\u914d\u7f6e\u8fc7\u7a0b\u4e2d\u5e94\u8be5\u907f\u514d\u768410\u4e2a\u574f\u4e60\u60ef\uff0c\u4f60\u4e2d\u62db\u4e86\u6ca1\uff1f\"<\/p>\n

7. Not stating the full information of your buffer in the method e.g.     weigh 5g of sodium phosphate into 1000ml of water<\/span><\/strong><\/p>\n

\u6ca1\u6709\u5728\u65b9\u6cd5\u4e2d\u8bf4\u660e\u4f60\u7684\u7f13\u51b2\u6db2\u7684\u5168\u90e8\u4fe1\u606f\uff0c\u4f8b\u5982\uff0c\u5c065g\u7684\u78f7\u9178\u94a0\u653e\u51651000ml\u7684\u6c34\u4e2d<\/span><\/strong><\/p>\n

\"\u6d41\u52a8\u76f8\u914d\u7f6e\u8fc7\u7a0b\u4e2d\u5e94\u8be5\u907f\u514d\u768410\u4e2a\u574f\u4e60\u60ef\uff0c\u4f60\u4e2d\u62db\u4e86\u6ca1\uff1f\"<\/span><\/strong><\/p>\n

The type of buffer (mono, di or tribasic)determines its pH buffering range.<\/span><\/p>\n

\u7f13\u51b2\u5242\u7684\u7c7b\u578b(\u5355\u76d0\u57fa\uff0c\u4e8c\u76d0\u57fa\uff0c\u4e09\u76d0\u57fa)\u51b3\u5b9a\u4e86\u5b83\u7684pH\u7f13\u51b2\u8303\u56f4\u3002<\/span><\/p>\n

The required molarity is what determines the buffer strength.5g of anhydrous sodium phosphate and 5g of monohydrate sodium phosphate willhave different buffer strengths and will affect retention.<\/span><\/p>\n

\u6240\u9700\u7684\u8d28\u91cf\u6d53\u5ea6\u51b3\u5b9a\u4e86\u7f13\u51b2\u5f3a\u5ea6\u30025g\u65e0\u6c34\u78f7\u9178\u6c22\u94a0\u548c5g\u4e00\u6c34\u5408\u78f7\u9178\u6c22\u94a0\u4f1a\u6709\u4e0d\u540c\u7684\u7f13\u51b2\u5f3a\u5ea6\uff0c\u5e76\u4f1a\u5f71\u54cd\u5230\u4fdd\u7559\u3002<\/span><\/p>\n

\"\u6d41\u52a8\u76f8\u914d\u7f6e\u8fc7\u7a0b\u4e2d\u5e94\u8be5\u907f\u514d\u768410\u4e2a\u574f\u4e60\u60ef\uff0c\u4f60\u4e2d\u62db\u4e86\u6ca1\uff1f\"<\/p>\n

8. Filling lines with organic without checking what was in there before<\/span><\/strong><\/p>\n

\u7528\u6709\u673a\u76f8\u7684\u586b\u5145\u7ba1\u8def\uff0c\u4e0d\u68c0\u67e5\u4e4b\u524d\u7684\u5185\u5bb9<\/span><\/strong><\/p>\n

\"\u6d41\u52a8\u76f8\u914d\u7f6e\u8fc7\u7a0b\u4e2d\u5e94\u8be5\u907f\u514d\u768410\u4e2a\u574f\u4e60\u60ef\uff0c\u4f60\u4e2d\u62db\u4e86\u6ca1\uff1f\"<\/span><\/strong><\/p>\n

If the previous method used buffer in line Band your method uses organic in line B there\u2019s a good chance you willprecipitate buffer in your pump tubing \/ pump head. I did it in my early daysand it caused a lot of damage.If in doubt \u2013 flush it out(80:20water : organic).<\/span><\/p>\n

\u5982\u679c\u524d\u4e00\u79cd\u65b9\u6cd5\u5728B\u7ba1\u8def\u4e2d\u4f7f\u7528\u4e86\u7f13\u51b2\u6db2\uff0c\u800c\u4f60\u7684\u65b9\u6cd5\u5728B\u7ba1\u8def\u4e2d\u4f7f\u7528\u4e86\u6709\u673a\u76f8\uff0c\u90a3\u4e48\u7f13\u51b2\u6db2\u5f88\u6709\u53ef\u80fd\u4f1a\u5728\u4f60\u7684\u6cf5\u7ba1\u8def\/\u6cf5\u5934\u4e2d\u6c89\u6dc0\u3002\u6211\u5728\u65e9\u671f\u5c31\u8fd9\u6837\u505a\u4e86\uff0c\u5e76\u4e14\u9020\u6210\u4e86\u5f88\u5927\u7684\u4f24\u5bb3\u3002\u5982\u679c\u6709\u6000\u7591\u2014\u2014\u628a\u5b83\u51b2\u51fa\u6765(\u752880:20\u6c34:\u6709\u673a\u76f8)\u3002<\/span><\/p>\n

\"\u6d41\u52a8\u76f8\u914d\u7f6e\u8fc7\u7a0b\u4e2d\u5e94\u8be5\u907f\u514d\u768410\u4e2a\u574f\u4e60\u60ef\uff0c\u4f60\u4e2d\u62db\u4e86\u6ca1\uff1f\"<\/p>\n

9. Propping up bottles to get last drop out<\/span><\/strong><\/p>\n

\u652f\u6491\u5230\u74f6\u5b50\u7684\u6700\u540e\u4e00\u6ef4\u88ab\u4f7f\u7528\u6389<\/span><\/strong><\/p>\n

\"\u6d41\u52a8\u76f8\u914d\u7f6e\u8fc7\u7a0b\u4e2d\u5e94\u8be5\u907f\u514d\u768410\u4e2a\u574f\u4e60\u60ef\uff0c\u4f60\u4e2d\u62db\u4e86\u6ca1\uff1f\"<\/span><\/strong><\/p>\n

It\u2019s 5 to 5 and you\u2019ve barely got enoughmobile phase to finish the run \u2013 it\u2019ll be running on fumes by the last fewsamples. Apart from the risk orrunning your pump and column dry, mobile phases evaporate from the surface, sothe mobile phase at the top of the bottle will have changed composition fromthe bulk. This portion from the top is exactly what will be running through thecolumn if you use the last dregs in the bottle.<\/span><\/p>\n

\u5b83\u662f5\u52305\uff0c\u4f60\u51e0\u4e4e\u6ca1\u6709\u8db3\u591f\u7684\u6d41\u52a8\u76f8\u6765\u5b8c\u6210\u8fd0\u884c\u2014\u2014\u6c14\u4f53\u5c06\u4f1a\u5728\u6700\u540e\u51e0\u4e2a\u6837\u54c1\u7684\u4e2d\u8fd0\u884c\u3002\u9664\u4e86\u78e8\u635f\u4f60\u7684\u6cf5\u548c\u8272\u8c31\u67f1\u8d70\u5e72\u7684\u98ce\u9669\uff0c\u6d41\u52a8\u76f8\u4ece\u8868\u9762\u84b8\u53d1\uff0c\u6240\u4ee5\u5728\u74f6\u5b50\u9876\u90e8\u7684\u6d41\u52a8\u76f8\u4e0e\u5927\u591a\u6570\u4f53\u79ef\u76f8\u6bd4\u6210\u5206\u5c06\u4f1a\u6539\u53d8\u3002\u5982\u679c\u4f60\u4f7f\u7528\u74f6\u5b50\u91cc\u7684\u6700\u540e\u5c11\u91cf\u7684\u6d41\u52a8\u76f8\uff0c\u9876\u90e8\u7684\u8fd9\u4e2a\u90e8\u5206\u6070\u597d\u662f\u8d2f\u7a7f\u67f1\u5b50\u7684\u4e1c\u897f\u3002<\/span><\/p>\n

\"\u6d41\u52a8\u76f8\u914d\u7f6e\u8fc7\u7a0b\u4e2d\u5e94\u8be5\u907f\u514d\u768410\u4e2a\u574f\u4e60\u60ef\uff0c\u4f60\u4e2d\u62db\u4e86\u6ca1\uff1f\"<\/p>\n

10. Using sonication to degas mobile phase<\/span><\/strong><\/p>\n

\u4f7f\u7528\u8d85\u58f0\u4eea\u5bf9\u6d41\u52a8\u76f8\u8fdb\u884c\u8131\u6c14<\/span><\/strong><\/p>\n

\"\u6d41\u52a8\u76f8\u914d\u7f6e\u8fc7\u7a0b\u4e2d\u5e94\u8be5\u907f\u514d\u768410\u4e2a\u574f\u4e60\u60ef\uff0c\u4f60\u4e2d\u62db\u4e86\u6ca1\uff1f\"<\/span><\/strong><\/p>\n

It\u2019s great for making sure all your buffersalts have dissolved, but it\u2019s the least effective method of degassing and itquickly heats up the mobile phase causing the organic portion to evaporate.Save yourself problems later \u2013take 5mins to vacuum filter your mobilephase\u2013 it degasses and filters in a single step.<\/span><\/p>\n

\u8fd9\u5bf9\u786e\u4fdd\u4f60\u6240\u6709\u7684\u7f13\u51b2\u76d0\u6eb6\u89e3\u90fd\u5f88\u597d\uff0c\u4f46\u8fd9\u662f\u6700\u4e0d\u6709\u6548\u7684\u8131\u6c14\u65b9\u6cd5\uff0c\u5b83\u4f1a\u8fc5\u901f\u52a0\u70ed\u6d41\u52a8\u76f8\uff0c\u5bfc\u81f4\u6709\u673a\u90e8\u5206\u84b8\u53d1\u3002\u8282\u7701\u4f60\u65e5\u540e\u7684\u95ee\u9898\u2014\u2014\u75285\u5206\u949f\u7684\u65f6\u95f4\u6765\u8fc7\u6ee4\u4f60\u7684\u6d41\u52a8\u76f8\u2014\u2014\u5b83\u4f1a\u5728\u4e00\u4e2a\u6b65\u9aa4\u4e2d\u8131\u6c14\u548c\u8fc7\u6ee4\u3002<\/span><\/p>\n

\"\u6d41\u52a8\u76f8\u914d\u7f6e\u8fc7\u7a0b\u4e2d\u5e94\u8be5\u907f\u514d\u768410\u4e2a\u574f\u4e60\u60ef\uff0c\u4f60\u4e2d\u62db\u4e86\u6ca1\uff1f\"<\/span><\/p>\n

\u4e0b\u56fe\u4e3a\u5404\u79cd\u8131\u6c14\u7684\u6548\u7387\u793a\u610f\u56fe\uff01<\/span><\/p>\n

\n

\u6765\u6e90\uff1a\u5206\u6790\u5b9e\u9a8c\u5ba4<\/span><\/p>\n


<\/p>\n<\/p><\/div>\n","protected":false},"excerpt":{"rendered":"

\u70b9\u51fb\u84dd\u8272\u5b57\u5173\u6ce8\u6211\u4eec\uff01 \u672c\u6587\u8f6c\u81ea\u201c\u836f\u7269\u5206\u6790\u4e4b\u5bb6\u201d\uff0c\u6b22\u8fce\u5173\u6ce8\uff01 HPLC Mobile Phases \u2013 10 b […]<\/p>\n","protected":false},"author":1,"featured_media":35150,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[2],"tags":[],"_links":{"self":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/35149"}],"collection":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/comments?post=35149"}],"version-history":[{"count":1,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/35149\/revisions"}],"predecessor-version":[{"id":35174,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/35149\/revisions\/35174"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media\/35150"}],"wp:attachment":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media?parent=35149"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/categories?post=35149"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/tags?post=35149"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}