{"id":43649,"date":"2018-09-02T01:00:00","date_gmt":"2018-09-17T07:17:16","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/2018\/09\/17\/%e4%b8%a4%e5%b9%b4%e4%ba%86%ef%bc%8c%e9%9f%a9%e6%98%a5%e9%9b%a8%e4%ba%8b%e4%bb%b6%e5%b0%98%e5%9f%83%e8%90%bd%e5%ae%9a%ef%bc%81\/"},"modified":"2018-09-17T15:17:39","modified_gmt":"2018-09-17T07:17:39","slug":"%e4%b8%a4%e5%b9%b4%e4%ba%86%ef%bc%8c%e9%9f%a9%e6%98%a5%e9%9b%a8%e4%ba%8b%e4%bb%b6%e5%b0%98%e5%9f%83%e8%90%bd%e5%ae%9a%ef%bc%81","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/43649\/","title":{"rendered":"\u4e24\u5e74\u4e86\uff0c\u97e9\u6625\u96e8\u4e8b\u4ef6\u5c18\u57c3\u843d\u5b9a\uff01"},"content":{"rendered":"
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\"\u4e24\u5e74\u4e86\uff0c\u97e9\u6625\u96e8\u4e8b\u4ef6\u5c18\u57c3\u843d\u5b9a\uff01\"<\/p>\n

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\u8c37 \u541b \u8bf4<\/strong><\/span><\/strong><\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n

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2018\u5e748\u670831\u65e5\uff0c\u6cb3\u5317\u79d1\u6280\u5927\u5b66\u53d1\u5e03\u4e86\u300a\u5b66\u6821\u516c\u5e03\u97e9\u6625\u96e8\u56e2\u961f\u64a4\u7a3f\u8bba\u6587\u7684\u8c03\u67e5\u548c\u5904\u7406\u7ed3\u679c\u300b\uff0c\u8ba4\u4e3a\u64a4\u7a3f\u8bba\u6587\u5df2\u4e0d\u518d\u5177\u5907\u91cd\u65b0\u53d1\u8868\u7684\u57fa\u7840\uff0c\u672a\u53d1\u73b0\u97e9\u6625\u96e8\u56e2\u961f\u6709\u4e3b\u89c2\u9020\u5047\u60c5\u51b5\u3002\u8fd9\u4e2a\u6301\u7eed\u4e24\u5e74\u591a\u7684\u97e9\u6625\u96e8\u8bba\u6587\u4e8b\u4ef6\u6700\u7ec8\u843d\u4e0b\u5e37\u5e55\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n


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 \u97e9\u6625\u96e8\u8bba\u6587\u4e8b\u4ef6\u7efc\u8ff0 <\/span><\/strong><\/p>\n

 \u6587\/towersimper<\/span><\/p>\n


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\"\u4e24\u5e74\u4e86\uff0c\u97e9\u6625\u96e8\u4e8b\u4ef6\u5c18\u57c3\u843d\u5b9a\uff01\"<\/p>\n


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2018\u5e749\u67081\u65e5<\/span><\/strong><\/span>\uff0c\u97e9\u6625\u96e8\u5c31\u6cb3\u5317\u79d1\u6280\u5927\u5b66\u516c\u5e03\u64a4\u7a3f\u8bba\u6587\u8c03\u67e5\u5904\u7406\u7ed3\u679c\u8868\u793a\u63a5\u53d7\uff0c\u5e76\u4e14\u8868\u793a\u5728\u56fd\u9645\u524d\u6cbf\u7684\u57fa\u56e0\u7f16\u8f91\u6280\u672f\u7814\u7a76\u9886\u57df\uff0c\u5b58\u5728\u8bb8\u591a\u4e0d\u53ef\u9884\u77e5\u7684\u95ee\u9898\u3002<\/span><\/p>\n


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\u8fd9\u4e00\u4e8b\u4ef6\u7684\u8d77\u56e0\u662f<\/span>2016\u5e745\u67082\u65e5<\/span><\/strong><\/span>\uff0c\u97e9\u6625\u96e8\u4f5c\u4e3a\u901a\u8baf\u4f5c\u8005\u5728\u300a\u81ea\u7136-\u751f\u7269\u6280\u672f\uff08<\/span>Nature Biotechnology<\/span><\/em><\/strong><\/span>\uff09\u300b\uff08\u7b80\u79f0NBT\uff09\u671f\u520a\u53d1\u8868\u4e86\u6807\u9898\u4e3a<\/span><\/p>\n

\u201cDNA-guided genome editing using the Natronobacterium gregoryi Argonaute\u201d\u7684\u8bba\u6587\u3002<\/span>
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\"\u4e24\u5e74\u4e86\uff0c\u97e9\u6625\u96e8\u4e8b\u4ef6\u5c18\u57c3\u843d\u5b9a\uff01\"<\/p>\n


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\u4f17\u6240\u5468\u77e5\uff0c\u57fa\u56e0\u7ec4\u7f16\u8f91\u6280\u672fCRISPR\/Cas9\u88ab\u300a\u79d1\u5b66\u300b\u6742\u5fd7\u5217\u4e3a2013\u5e74\u5e74\u5ea6<\/span>\u5341\u5927\u79d1\u6280\u8fdb\u5c55\u4e4b\u4e00<\/span><\/strong>\uff0c\u53d7\u5230\u4eba\u4eec\u7684\u9ad8\u5ea6\u91cd\u89c6\u3002<\/span><\/p>\n


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\u4f46\u5979\u4ecd\u7136\u5b58\u5728\u4e00\u4e9b\u4e0d\u8db3\u4e4b\u5904\uff0c\u5982CRISPR\/Cas9\u7cfb\u7edf\u4ecd\u7136\u4f1a\u5728\u8131\u9776\u4f4d\u70b9\uff08\u5728\u8fd9\u4e9b\u4f4d\u70b9\u4e0a\uff0cgRNA\u4e0e\u9776DNA\u5e8f\u5217\u4e4b\u95f4\u5b58\u5728\u9519\u914d\uff09\u8fdb\u884c\u5207\u5272\uff0c\u8fd9\u79cd\u8131\u9776\u5207\u5272\u6709\u53ef\u80fd\u5bfc\u81f4\u5f88\u591a\u6709\u5bb3\u7684\u540e\u679c\uff0c\u5982<\/span>\u764c\u75c7<\/span><\/strong>\u4ea7\u751f\uff1bgRNA\u5bb9\u6613\u5f62\u6210<\/span>\u4e8c\u7ea7\u7ed3\u6784<\/span><\/strong>\uff0c\u7b49\u7b49\u3002<\/span><\/p>\n


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\u5728\u97e9\u6625\u96e8\u7684\u8fd9\u7bc7\u8bba\u6587\u4e2d\uff0c\u4ed6\u53d1\u73b0\uff0c\u7c7b\u4f3c\u4e8eCas9\uff0c\u6765\u81eaArgonaute\u86cb\u767d\u5bb6\u65cf\u7684\u6838\u9178\u5185\u5207\u9176\u4e5f\u5229\u7528\u5be1\u6838\u82f7\u9178\u4f5c\u4e3a\u5411\u5bfc\u964d\u89e3\u5165\u4fb5\u7684\u57fa\u56e0\u7ec4\u3002<\/span><\/p>\n


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\u5177\u4f53\u800c\u8a00\uff0c\u4ed6\u4eec\u53d1\u73b0\u6765\u81ea\u683c\u6c0f\u55dc\u76d0\u78b1\u6746\u83cc\uff08Natronobacterium gregoryi\uff09\u7684\u4e00\u79cdArgonaute\u86cb\u767d\uff08NgAgo\uff09\u4f5c\u4e3a\u4e00\u79cd<\/span>\u6838\u9178\u5185\u5207\u9176<\/span><\/strong>\uff0c\u5728\u5411\u5bfcDNA\uff08guide DNA, gDNA\uff09\u7684\u5f15\u5bfc\u4e0b\uff0c\u80fd\u591f\u5728\u4eba\u7ec6\u80de\u4e2d\u8fdb\u884c\u57fa\u56e0\u7ec4\u7f16\u8f91\u3002<\/span><\/p>\n


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\u4e0d\u4ec5\u5982\u6b64\uff0c\u66f4\u91cd\u8981\u7684\u662f\u8fd9\u79cdNgAgo-gDNA\u7cfb\u7edf\u8bbe\u8ba1\u65b9\u4fbf\uff0cgDNA\u53ef\u76f4\u63a5\u8f6c\u67d3\u7ec6\u80de\uff0c\u800c\u65e0\u9700\u6784\u5efa\u4e13\u95e8\u7684gDNA\u8868\u8fbe\u8f7d\u4f53\uff1bNgAgo\u53ef\u7f16\u8f91\u57fa\u56e0\u7ec4\u5185\u4efb\u4f55\u4f4d\u70b9\uff0c\u800cCas9\u7684\u57fa\u56e0\u7ec4\u9776\u70b9\u5fc5\u987b\u4f4d\u4e8ePAM\u5e8f\u5217\u7684\u4e0a\u6e38\uff0c\u800c\u4e14\u4e0d\u80fd\u5bcc\u542bG+C\uff1b\u6240\u4f7f\u7528\u7684gDNA\u662fDNA\u800c\u975eRNA\uff0c\u4e0d\u4f1a\u50cfRNA\u90a3\u6837\u5bb9\u6613\u5f62\u6210\u4e8c\u7ea7\u7ed3\u6784\u800c\u5bfc\u81f4\u5931\u6548\u6216\u8131\u9776\u6548\u5e94\uff1b\u5bf9\u6e38\u79bb\u4e8e\u7ec6\u80de\u6838\u7684DNA\u5177\u6709\u66f4\u9ad8\u7684\u5207\u5272\u6548\u7387\u3002<\/span><\/p>\n


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\u7531\u6b64\u53ef\u77e5\uff0c\u76f8\u6bd4\u8f83\u4e8eCas9-sgRNA\uff0c<\/span>NgAgo-gDNA<\/span><\/strong>\u5177\u6709\u66f4\u5927\u7684<\/span>\u4f18\u52bf<\/span><\/strong>\uff0c\u89c4\u907f\u4e86\u4ee4\u4eba\u5934\u75db\u7684\u8131\u9776\u6548\u5e94\uff0c\u5176\u5e94\u7528\u524d\u666f\u662f\u4e0d\u8a00\u800c\u55bb\u7684\u3002<\/span><\/p>\n


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\u66f4\u96be\u80fd\u53ef\u8d35\u7684\u662f\uff0c\u5728Cas9-sgRNA\u6210\u4e3a\u5168\u4e16\u754c\u5404\u5927\u5b9e\u9a8c\u5ba4\u4e89\u76f8\u4f7f\u7528\u7684\u9999\u997d\u997d\u65f6\uff0c\u8fd9\u9879\u7814\u7a76\u53d1\u73b0\u7684\u65b0\u57fa\u56e0\u7f16\u8f91\u7cfb\u7edf\u5177\u6709\u5982\u6b64\u5de8\u5927\u7684\u4f18\u52bf\uff0c\u5411\u5b83\u53d1\u51fa\u6709\u529b\u7684\u6311\u6218\uff0c\u4e5f\u96be\u602a\u4f1a\u5728\u56fd\u5185\u5f15\u53d1\u4e00\u9635\u70ed\u6f6e\u3002<\/span><\/p>\n


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\"\u4e24\u5e74\u4e86\uff0c\u97e9\u6625\u96e8\u4e8b\u4ef6\u5c18\u57c3\u843d\u5b9a\uff01\"<\/p>\n

\u56fe\u7247\u6765\u6e90\uff1aTwitter<\/span><\/p>\n


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2016\u5e745\u670826\u65e5<\/span><\/strong><\/span>\uff0c\u201c\u672a\u540d\u7a7a\u95f4\u201d\u8bba\u575b\u4e0a\u51fa\u73b0\u4e86\u201c\u7eaf\u4ece\u79d1\u5b66\u89d2\u5ea6\u5206\u6790\u97e9\u6625\u96e8\u7684\u6587\u7ae0\u201d\u7684\u5e16\u5b50\uff0c\u8ba4\u4e3a\u97e9\u6625\u96e8\u7684\u8bba\u6587\u4eceAgo\u7406\u8bba\u4e0a\u770b\u884c\u4e0d\u901a\u3002\u6b64\u540e\uff0c\u5728\u767e\u5ea6\u8d34\u5427\u3001\u77e5\u4e4e\u7b49\u8bba\u575b\u4e0a\uff0c\u7c7b\u4f3c\u8d28\u7591\u4e0d\u65ad\u51fa\u73b0\u3002<\/span><\/p>\n


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2016\u5e746\u670830\u65e5<\/span><\/strong><\/span>\uff0c\u65b9\u821f\u5b50\u53d1\u6587\u8d28\u7591\u97e9\u6625\u96e8\uff1a\u201c\u636e\u542c\u62a5\u544a\u7684\u4eba\u8bf4\uff0c\u97e9\u6625\u96e8\u5728\u5317\u5927\u548c\u9057\u4f20\u6240\u7684\u62a5\u544a\u4e0a\u90fd\u5f3a\u8c03\uff0c\u4ed6\u76ee\u524d\u7684NgAgo\u662f\u521d\u7ea7\u7248\u3001\u9700\u8981\u9ad8\u8d85\u7684\u5b9e\u9a8c\u6280\u5de7\u3001\u7b49\u4ed6\u63a8\u51fa2.0\u7248\u548cSmart\u7248\u3002\u8fd9\u4e9b\u8bf4\u6cd5\u8ddf\u4ed6\u5728\u8bba\u6587\u91cc\u7684\u63cf\u8ff0\u662f\u77db\u76fe\u7684\u3002<\/span><\/p>\n


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\u56e0\u4e3a\u4ed6\u63cf\u8ff0\u7684\u53ea\u662f\u4e2a\u5e76\u4e0d\u590d\u6742\u7684\u8f6c\u67d3\u5b9e\u9a8c\uff0cT7E1\u548c\u6d4b\u5e8f\u4e5f\u90fd\u662f\u73b0\u6210\u7684\u6280\u672f\uff0c\u5e76\u4e0d\u9700\u8981\u9ad8\u8d85\u7684\u5b9e\u9a8c\u6280\u5de7\uff0c\u6309\u7167\u5176\u63d0\u4f9b\u7684\u6b65\u9aa4\u5e94\u8be5\u662f\u4e0d\u96be\u88ab\u91cd\u590d\u51fa\u6765\u7684\u3002\u201d<\/span><\/p>\n


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2016\u5e747\u67082\u65e5<\/span><\/strong><\/span>\uff0c\u5728\u8d34\u6709\u65b9\u821f\u5b50\u8d28\u7591\u539f\u6587\u7684\u5e16\u5b50\u4e2d\uff0c\u97e9\u6625\u96e8\u8fdb\u884c\u4e86\u8f83\u4e3a\u8be6\u7ec6\u7684\u56de\u5e94\uff0c\u5f3a\u8c03<\/span>NgAgo\u7cfb\u7edf<\/span><\/strong>\u5bf9\u6c61\u67d3\u7279\u522b<\/span>\u654f\u611f<\/span>\uff0c\u5b9e\u9a8c\u65f6\u4e0d\u8981\u6709\u5bc4\u751f\u83cc\u548c\u652f\u539f\u4f53\u7684\u6c61\u67d3\uff0c\u5e76\u5bf9\u5b9e\u9a8c\u73af\u8282\u505a\u51fa\u6280\u672f\u6027\u7684\u5efa\u8bae\u3002<\/span><\/p>\n


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\u4e0e\u6b64\u540c\u65f6\uff0c\u97e9\u6625\u96e8\u5728\u767e\u5ea6\u8d34\u5427\u201c\u56fd\u9645\u7c73\u5170\u5427\u201d\u56de\u590d\u7f51\u7edc\u4e0a\u7684\u8d28\u7591\u3002\u4f46\u968f\u540e\u4ed6\u4fbf\u505c\u6b62\u5728\u7f51\u7edc\u4e0a\u53d1\u58f0\uff0c\u5e76\u5f00\u59cb<\/span>\u62d2\u7edd\u91c7\u8bbf<\/span><\/strong>\u3002<\/span><\/p>\n


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2016\u5e747\u670821\u65e5<\/span><\/strong><\/span>\uff0c\u4e2d\u56fd\u79d1\u5b66\u9662\u4e0a\u6d77\u795e\u7ecf\u79d1\u5b66\u7814\u7a76\u6240\u7814\u7a76\u5458\u4ec7\u5b50\u9f99\u5728\u81ea\u5df1\u7684\u5fae\u535a\u8ba4\u8bc1\u8d26\u53f7\u201c\u6c42\u5bfc\u201d\u4e0a\u53d1\u6587\uff0c\u8868\u793a\u5b9e\u9a8c\u5ba4\u4ecd\u5728\u91cd\u590d\u97e9\u6625\u96e8\u7684\u5b9e\u9a8c\uff0c\u4f18\u5316\u5404\u79cd\u6761\u4ef6\uff0c\u76ee\u524d\u7684\u5b9e\u9a8c\u7ed3\u679c\u8ddd\u79bb\u97e9\u6625\u96e8\u8bba\u6587\u4e2d\u7684\u7ed3\u679c\u201c\u76f8\u5dee\u751a\u8fdc\u201d\uff0c\u5e76\u547c\u5401\u97e9\u6625\u96e8\u63d0\u4f9b\u201c\u53ef\u91cd\u590dNBT\u53d1\u8868\u6587\u7ae0\u7684NgAgo\uff0c\u6216\u8005\u4f18\u5316\u7684Ngago2.0\uff0csmart\u7248\u672c\u7b49\u7b49\u201d\u3002<\/span><\/p>\n


<\/span><\/p>\n

2016\u5e747\u670829\u65e5<\/span><\/strong><\/span>\uff0c\u6fb3\u5927\u5229\u4e9a\u79d1\u5b66\u5bb6Gaetan Burgio\u5728\u63a8\u7279\u4e0a\u53d1\u5e03\u957f\u6587\u300a\u6211\u7684NgAgo\u7ecf\u5386\u300b\uff0c\u8868\u793a\u4e0d\u80fd\u91cd\u590d\u97e9\u6625\u96e8\u8bba\u6587\u56fe4\u7684\u7ed3\u679c\uff1b\u56fd\u9645\u8f6c\u57fa\u56e0\u6280\u672f\u534f\u4f1a\u5219\u7ed9\u4f1a\u5458\u7fa4\u53d1\u90ae\u4ef6\uff0c\u544a\u8beb\u5927\u5bb6\u201c<\/span>NgAgo\u65e0\u6cd5\u5728\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u4e2d\u8fdb\u884c\u57fa\u56e0\u7f16\u8f91<\/span><\/strong>\u3002\u4e0d\u8981\u518d\u6d6a\u8d39\u65f6\u95f4\u3001\u91d1\u94b1\u3001\u4eba\u529b\u548c\u8bfe\u9898\u3002\u201d<\/span><\/p>\n


<\/span><\/p>\n

2016\u5e747\u670831\u65e5<\/span><\/strong><\/span>\uff0c\u6765\u81ea\u6fb3\u5927\u5229\u4e9a\u3001\u7f8e\u56fd\u3001\u897f\u73ed\u7259\u7b49\u56fd\u7684\u79d1\u5b66\u5bb6\u5728\u63a8\u7279\u4e0a\u6307\u51fa\uff0c\u65e0\u6cd5\u91cd\u590d\u97e9\u6625\u96e8NgAgo\u7cfb\u7edf\u7684\u57fa\u56e0\u7f16\u8f91\u7ed3\u679c\u3002\u8fd9\u4e9b\u58f0\u97f3\u520a\u767b\u5728\u7531<\/span>\u989c\u5b81\u3001\u6587\u5c0f\u521a\u3001\u5218\u514b\u5cf0<\/span><\/strong>\u4e09\u4f4d\u56fd\u9645\u8457\u540d\u79d1\u5b66\u5bb6\u62c5\u4efb\u4e3b\u7f16\u7684\u300a\u8d5b\u5148\u751f\u300b\u79d1\u5b66\u65b0\u5a92\u4f53\u5e73\u53f0\u4e0a\u3002<\/span><\/p>\n


<\/span><\/strong><\/span><\/p>\n

2016\u5e748\u67088\u65e5<\/span><\/strong><\/span>\uff0c\u300a\u81ea\u7136\u300b\u6742\u5fd7\u505a\u4e86\u5173\u4e8eNgAgo\u7684\u65b0\u95fb\u62a5\u9053\uff0c\u79f0\u6709\u4e0d\u613f\u900f\u9732\u59d3\u540d\u7684\u79d1\u5b66\u5bb6\u5411\u300a\u81ea\u7136\u300b\u6742\u5fd7\u901a\u8baf\u5458David Cyranoski\u8bc1\u5b9e\u4e86\u5b9e\u9a8c\u7684\u53ef\u91cd\u590d\u6027\u3002<\/span><\/p>\n


<\/span><\/p>\n

\u97e9\u6625\u96e8\u5728\u968f\u540e\u63a5\u53d7\u5a92\u4f53\u91c7\u8bbf\u65f6\u8868\u793a\uff0c\u201c\u300a\u81ea\u7136\u300b\u7684\u7ed3\u679c\u5c31\u662f\uff0c\u8fd9\u4e2a\u5b9e\u9a8c\u662f\u53ef\u4ee5\u91cd\u590d\u7684\uff0c\u4f46\u6709\u7684\u80fd\u591f\u505a\u51fa\u6765\uff0c\u6709\u7684\u505a\u4e0d\u51fa\u6765\u3002\u201d\uff0c\u800c\u201c\u8c03\u67e5\u8bbf\u8c08\u7ed3\u679c\u5df2\u7ecf\u76f8\u5f53\u4e8e\u662f\u516c\u5f00\u56de\u5e94\u4e86\u3002\u201d<\/span><\/p>\n


<\/span><\/p>\n

\u4e0d\u8fc7\uff0cCyranoski\u5bf9\u6b64\u56de\u590d\u9053\uff0c\u8fd9\u7bc7\u662f\u62a5\u9053\uff0c\u4e0d\u662f\u5b66\u672f\u8bba\u6587\uff0c\u201c\u4e0d\u5e94\u8be5\u88ab\u4f5c\u4e3a\u97e9\u6625\u96e8\u5b9e\u9a8c\u53ef\u91cd\u590d\u7684\u8bc1\u636e\u201d\u3002\u4ed6\u5199\u9053\uff1a\u201c\u6211\u6ca1\u6709\u8fdb\u884c\u4efb\u4f55\u72ec\u7acb\u7684\u91cd\u590d\u5b9e\u9a8c\uff0c\u56e0\u6b64\u5bf9\u4e8e\u8fd9\u6b21\u8c03\u67e5\u7ed3\u679c\u65e0\u6cd5\u589e\u52a0\u4efb\u4f55\u7b2c\u4e00\u624b\u4fe1\u606f\u3002\u6211\u7684\u62a5\u9053\u786e\u5b9e\u53cd\u6620\u7684\u662f\u5173\u4e8e\u97e9\u6625\u96e8\u7684\u5b9e\u9a8c\u53ef\u91cd\u590d\u6027\u6709\u4e0d\u540c\u7684\u89c2\u70b9\u3002\u800c\u4e14\u636e\u6211\u6240\u77e5\uff0c\u8fd9\u4e2a\u4e89\u8bae\u5230\u73b0\u5728\u8fd8\u6ca1\u6709\u89e3\u51b3\u3002\u201d<\/span><\/p>\n


<\/span><\/p>\n

\u9274\u4e8e\u79d1\u7814\u8bba\u6587\u662f\u5426\u5177\u6709\u53ef\u91cd\u590d\u6027\uff0c\u662f\u79d1\u5b66\u7814\u7a76\u7684\u91cd\u8981\u57fa\u7840\u4e4b\u4e00\u3002\u9762\u5bf9\u8d28\u7591\uff0c\u300a\u81ea\u7136-\u751f\u7269\u6280\u672f\u300b\u5728<\/span>2016\u5e7411\u670819\u65e5<\/span><\/strong>\u5ba3\u5e03\uff0c\u201c<\/span>\u4ecb\u5165\u8bba\u6587\u8c03\u67e5<\/span><\/strong>\u201d\u3002<\/span><\/p>\n


<\/span><\/p>\n

2016\u5e7410\u670810\u65e5<\/span><\/strong><\/span>\uff0c\u7531\u5317\u4eac\u5927\u5b66\u6559\u6388<\/span>\u9b4f\u6587\u80dc\u7ec4\u7ec7<\/span><\/strong>\u548c\u53d1\u8d77\uff0c\u6765\u81ea\u4e2d\u56fd\u79d1\u5b66\u9662\u7b49\u79d1\u7814\u9662\u6240\u7684<\/span>13\u4f4d<\/span><\/strong>\u79d1\u5b66\u5bb6\u901a\u8fc7\u4e24\u5bb6\u5a92\u4f53\uff0c\u5b9e\u540d\u53d1\u5e03\u58f0\u660e\uff0c\u201c\u6ca1\u80fd\u2018\u91cd\u590d\u2019\u97e9\u6625\u96e8\u7684\u5b9e\u9a8c\uff0c\u2014\u2014\u547c\u5401\u6709\u5173\u65b9\u9762\u7ec4\u7ec7\u7b2c\u4e09\u65b9\u4ecb\u5165\u8c03\u67e5\u201d\u3002<\/span><\/p>\n


<\/span><\/p>\n

2016\u5e7411\u670816\u65e5<\/span><\/strong><\/span>\uff0c\u56fd\u5185\u591620\u4f4d\u751f\u7269\u5b66\u5bb6\u8054\u540d\u5728\u56fd\u9645\u671f\u520a\u300a\u86cb\u767d\u8d28\u4e0e\u7ec6\u80de\u300b\u4e0a\u53d1\u8868\u5b66\u672f\u901a\u8baf\u300a\u5173\u4e8eNgAgo\u7684\u7591\u95ee\u300b\uff0c\u8d28\u7591\u97e9\u6625\u96e8\u56e2\u961f\u8be5\u8bba\u6587\u7684\u53ef\u91cd\u590d\u6027\u3002<\/span><\/p>\n


<\/span><\/p>\n

20\u4f4d<\/span><\/strong>\u5b66\u8005\u5728\u5404\u81ea\u7684\u5b9e\u9a8c\u5ba4\u8fdb\u884c\u4e86\u91cd\u590d\u5b9e\u9a8c\uff0c\u4f46\u5728\u4e0d\u540c\u7ec6\u80de\u7cfb\u548c\u751f\u7269\u4e2d<\/span>\u65e0\u6cd5\u68c0\u6d4b<\/span><\/strong>\u5230NgAgo\u6280\u672f\u6240\u4ea7\u751f\u7684\u57fa\u56e0\u7f16\u8f91\u73b0\u8c61\u3002<\/span><\/p>\n


<\/span><\/p>\n

2016\u5e7411\u670819\u65e5<\/span><\/strong><\/span>\uff0c\u300a\u81ea\u7136-\u751f\u7269\u6280\u672f\u300b\u8868\u7ebf\u4e0a\u58f0\u660e\uff0c\u8868\u8fbe\u4e86\u4ee5\u4e0b\u56db\u70b9\uff1a<\/span><\/p>\n


<\/span><\/p>\n

(1) NBT\u520a\u767b\u7ecf\u540c\u884c\u8bc4\u8bae\u7684<\/span>\u7f8e\u56fd\u3001\u5fb7\u56fd\u3001\u97e9\u56fd<\/span>9\u5bb6<\/span><\/strong>\u5355\u4f4d\u8054\u5408\u8bc4\u8bba\u6587\u7ae0\u201cFailure to detect DNA-guided genome editing using Natronobacterium gregoryi Argonaute\u201d\uff0c\u5373\u65e0\u6cd5\u5b9e\u73b0NgAgo\u57fa\u56e0\u7f16\u8f91\u529f\u80fd\uff1b<\/span><\/p>\n


<\/span><\/p>\n

\uff082\uff09\u5bf9\u97e9\u6625\u96e8\u8bba\u6587\u8868\u8fbe\u7f16\u8f91\u5173\u5207\uff0c\u5373Editorial Expression of Concern\uff1b<\/span><\/p>\n


<\/p>\n

\uff083\uff09<\/span>\u8bbe\u5b9a2017\u5e741\u6708\u671f\u9650<\/span><\/strong>\uff0c\u9650\u4f5c\u8005\u4e88\u4ee5\u6f84\u6e05\u8bf4\u660e\uff0c\u201cprovide them with the opportunity to do that by January 2017\u201d\uff1b<\/span><\/p>\n


<\/p>\n

(4) \u97e9\u6625\u96e8\u4e0e\u6c88\u5578\u540c\u610fEditorial Expression of Concern\uff0c\u4f46\u53e6\u5916\u4e09\u4f4d\u4f5c\u8005\u53cd\u5bf9\uff01<\/span><\/p>\n


<\/span><\/p>\n

2017\u5e745\u67089\u65e5<\/span><\/strong><\/span>\uff0c\u300a\u81ea\u7136-\u751f\u7269\u6280\u672f\u300b\u5728\u7ebf\u53d1\u5e03\u201c\u7f16\u8f91\u90e8\u5173\u5207\u201d(Addendum: Editorial Expression of Concern)\uff0c\u8868\u660e\u671f\u520a\u7f16\u8f91\u6ce8\u610f\u5230\u4e86\u8bfb\u8005\u4eec\u5bf9\u97e9\u6625\u96e82016\u5e745\u67082\u65e5\u5728\u7ebf\u53d1\u8868\u7684\u6709\u5173NgAgo\u8bba\u6587\u91cd\u590d\u6027\u7684\u62c5\u5fe7\u3002<\/span><\/p>\n


<\/span><\/p>\n

\u6b64\u524dNBT\u6742\u5fd7\u4e5f\u53d1\u5e03\u4e86\u7531<\/span>\u97e9\u56fd\u9996\u5c14\u57fa\u7840\u7814\u7a76\u6240\u3001\u5f17\u83b1\u5821\u5927\u5b66\u3001\u7f8e\u56fdMayo\u533b\u5b66\u4e2d\u5fc3<\/span><\/strong>\u4e09\u4e2a\u8bfe\u9898\u7ec4\u9898\u4e3a\u201cFailure to detect DNA-guided genome editing using Natronobacterium gregoryi Argonaute\u201d\u7684\u6587\u7ae0\uff0c\u8bd5\u56fe\u91cd\u590d\u97e9\u6625\u96e8\u8bba\u6587\u4e2d\u7684Figure 4\uff0c\u7136\u540e\u5e76<\/span>\u6ca1\u6709\u68c0\u6d4b\u5230<\/span><\/strong>NgAgo\u4ecb\u5bfc\u7684DNA\u5207\u5272\u3002<\/span><\/p>\n


<\/span><\/p>\n

NBT\u7f16\u8f91\u90e8\u5df2\u7ecf\u4e0e\u97e9\u6625\u96e8\u7b49\u4f5c\u8005\u8fdb\u884c\u8054\u7cfb\uff0c\u5e76\u8868\u793a\u97e9\u6625\u96e8\u4ed6\u4eec\u8fd8\u5728\u8c03\u67e5\u8fd9\u4e00\u7cfb\u5217\u7f3a\u5c11\u91cd\u590d\u6027\u7684\u539f\u56e0\u3002\u76f8\u5173\u4f5c\u8005\u5df2\u7ecf\u77e5\u6653\u8fd9\u4efd\u58f0\u660e\uff0c\u8c03\u67e5\u8fd8\u5728\u7ee7\u7eed\u4e2d\uff0c\u97e9\u6625\u96e8\u548c\u6c88\u5578\u4e5f\u540c\u610f\u4e86NBT\u7f16\u8f91\u90e8\u7684\u8fd9\u4e00\u5173\u5207\u4e0e\u58f0\u660e\u3002<\/span><\/p>\n


<\/span><\/p>\n

\u4f46\u662f\u8bba\u6587\u7684\u5176\u4ed6\u51e0\u4f4d\u4f5c\u8005\u9ad8\u5cf0\u3001Jiang Feng\u548cYongqiang Wu\u89c9\u5f97\u8fd9\u4e2a\u65f6\u5019\u53d1\u5e03\u5173\u5207\u4e0d\u5408\u9002\u3002\u7f16\u8f91\u90e8\u6700\u540e\u8868\u793a\uff0c\u4e00\u65e6\u5b8c\u6210\u76f8\u5173\u8c03\u67e5\uff0c\u5c06\u4f1a\u516c\u5e03\u8fd9\u4e9b\u7ed3\u679c\u3002<\/span><\/p>\n


<\/span><\/p>\n

2017\u5e748\u67083\u65e5<\/span><\/strong><\/span>\uff0c\u97e9\u6625\u96e8\u56e2\u961f\u4e3b\u52a8\u64a4\u56de\u8be5\u8bba\u6587\u3002\u4e0e\u6b64\u540c\u65f6\uff0c\u6cb3\u5317\u79d1\u6280\u5927\u5b66\u5b98\u7f51\u7684\u58f0\u660e\u79f0\uff0c\u9274\u4e8e\u8be5\u8bba\u6587\u5df2\u64a4\u7a3f\uff0c\u201c\u5b66\u6821\u51b3\u5b9a\u542f\u52a8\u5bf9\u97e9\u6625\u96e8\u8be5\u9879\u7814\u7a76\u6210\u679c\u7684\u5b66\u672f\u8bc4\u8bae\u53ca\u76f8\u5173\u7a0b\u5e8f\u201d\u3002<\/span><\/p>\n


<\/span><\/p>\n

\u800c\u97e9\u6625\u96e8\u56e2\u961f\u540c\u65f6\u5ba3\u5e03\uff0c\u5c06\u8fdb\u4e00\u6b65\u7814\u7a76\u4e0d\u80fd\u91cd\u590d\u7684\u539f\u56e0\uff0c\u5e76\u540c\u610f\u6309\u5b66\u6821\u5b89\u6392\u9009\u62e9\u4e00\u5bb6\u7b2c\u4e09\u65b9\u5b9e\u9a8c\u5ba4\uff0c\u5728\u540c\u884c\u4e13\u5bb6\u652f\u6301\u4e0b\u5f00\u5c55\u5b9e\u9a8c\uff0c\u9a8c\u8bc1NgAgo-gDNA\u57fa\u56e0\u7f16\u8f91\u7684\u6709\u6548\u6027\uff0c\u5e76\u5c06\u5b9e\u9a8c\u7ed3\u679c\u516c\u5e03\uff0c\u4ee5\u56de\u5e94\u793e\u4f1a\u5173\u5207\u3002<\/span><\/p>\n


<\/span><\/p>\n

\u5982\u4eca\uff0c\u6cb3\u5317\u79d1\u6280\u5927\u5b66\u7ec8\u4e8e\u516c\u5e03\u5904\u7406\u7ed3\u679c\uff0c\u8ba4\u4e3a\u672a\u53d1\u73b0\u97e9\u6625\u96e8\u56e2\u961f\u6709\u4e3b\u89c2\u9020\u5047\u60c5\u51b5\u3002\u60f3\u5fc5\u8fd9\u4e00\u7ed3\u8bba\u4f1a\u5f15\u53d1\u5f88\u591a\u4eba\u5410\u69fd\u3002<\/span><\/p>\n


<\/span><\/p>\n

\u6709\u4e9b\u4eba\u8ba4\u4e3a\u97e9\u6625\u96e8\u5728\u56de\u590d\u7f51\u53cb\u548c\u5176\u4ed6\u79d1\u5b66\u5bb6\u7684\u8d28\u7591\u65f6\uff0c\u4e0d\u8bda\u5b9e\u3002\u6709\u4e9b\u4eba\u8ba4\u4e3a\u6cb3\u5317\u79d1\u6280\u5927\u5b66\u7684\u6700\u7ec8\u5904\u7406\u51b3\u5b9a\u6709\u548c\u7a00\u6ce5\u7684\u5acc\u7591\uff0c<\/span>\u4e0d\u8db3\u4ee5\u5236\u6b62<\/span><\/strong>\u79d1\u7814\u673a\u6784\u7684<\/span>\u5b66\u672f\u4e0d\u7aef\u4e4b\u98ce<\/span><\/strong>\u3002\u6709\u4e9b\u4eba\u8ba4\u4e3a\u8fd9\u662f\u79d1\u5b66\u53d1\u5c55<\/span>\u5fc5\u987b\u627f\u53d7\u7684\u4ee3\u4ef7<\/span><\/strong>\u3002<\/span><\/p>\n


<\/p>\n

\"\u4e24\u5e74\u4e86\uff0c\u97e9\u6625\u96e8\u4e8b\u4ef6\u5c18\u57c3\u843d\u5b9a\uff01\"<\/p>\n


<\/p>\n

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\n


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