{"id":49247,"date":"2018-09-22T01:00:00","date_gmt":"2018-09-26T01:56:38","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/2018\/09\/26\/lnp%ef%bc%9a%e4%bf%83%e8%bf%9brnai%e7%94%b1%e7%90%86%e8%ae%ba%e5%88%b0%e4%b8%b4%e5%ba%8a%e5%ba%94%e7%94%a8\/"},"modified":"2018-09-26T09:56:41","modified_gmt":"2018-09-26T01:56:41","slug":"lnp%ef%bc%9a%e4%bf%83%e8%bf%9brnai%e7%94%b1%e7%90%86%e8%ae%ba%e5%88%b0%e4%b8%b4%e5%ba%8a%e5%ba%94%e7%94%a8","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/49247\/","title":{"rendered":"LNP\uff1a\u4fc3\u8fdbRNAi\u7531\u7406\u8bba\u5230\u4e34\u5e8a\u5e94\u7528"},"content":{"rendered":"<div class=\"bpp-post-content\">\n<p><img class=\"__bg_gif\"     width=\"auto\" style=\"font-size: 16px;white-space: normal;color: rgb(62, 62, 62);line-height: 1.75em;text-align: center;background-color: rgb(255, 255, 255);box-sizing: border-box !important;word-wrap: break-word !important;visibility: visible !important;width: auto !important;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/09\/6-1537926998.gif\" alt=\"LNP\uff1a\u4fc3\u8fdbRNAi\u7531\u7406\u8bba\u5230\u4e34\u5e8a\u5e94\u7528\" title=\"LNP\uff1a\u4fc3\u8fdbRNAi\u7531\u7406\u8bba\u5230\u4e34\u5e8a\u5e94\u7528\"  \/><\/p>\n<section  label=\"Powered by 135editor.com\" style=\"font-size:16px;font-family:\u5fae\u8f6f\u96c5\u9ed1;\">\n<section  label=\"Powered by 135editor.com\">\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"padding-top: 10px;padding-bottom: 10px;box-sizing: border-box;\">\n<section style=\"width: 100%;border-top: 1px solid rgb(170, 170, 171);border-bottom: 1px solid rgb(170, 170, 171);box-sizing: border-box;\" >\n<section style=\"width: 45px;padding-right: 5px;padding-left: 5px;margin-top: -14px;box-sizing: border-box;\"><img class=\"\"      style=\"width: 100%;margin-left: -5px;background: none rgb(156, 218, 245);\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/09\/5-1537926998.png\" alt=\"LNP\uff1a\u4fc3\u8fdbRNAi\u7531\u7406\u8bba\u5230\u4e34\u5e8a\u5e94\u7528\" title=\"LNP\uff1a\u4fc3\u8fdbRNAi\u7531\u7406\u8bba\u5230\u4e34\u5e8a\u5e94\u7528\"  \/><\/section>\n<section style=\"font-size: 15px;color: rgb(63, 62, 63);text-align: justify;line-height: 25px;box-sizing: border-box;\" class=\"135brush\">\n<p style=\"line-height: 1.75em;\"><span style=\"color: rgb(0, 128, 255);\"><strong>\u5bfc\u8bfb\uff1a<\/strong><\/span><span style=\"color: rgb(0, 0, 0);\">8\u670811\u65e5\uff0c<strong>\u7f8e\u56fdFDA\u5ba3\u5e03\u9996\u6b3e\u5c0f\u5e72\u6270RNA (siRNA)\u836f\u7269Onpattro (patisiran)\u4e0a\u5e02\uff0c\u8be5\u836f\u7269\u66fe\u83b7\u5f97\u7f8e\u56fdFDA\u6388\u4e88\u7684\u7a81\u7834\u6027\u7597\u6cd5\u8ba4\u5b9a\u3001\u4f18\u5148\u5ba1\u8bc4\u8d44\u683c\u3001\u5feb\u901f\u901a\u9053\u8d44\u683c\u548c\u5b64\u513f\u836f\u8d44\u683c<\/strong>\u3002\u5b83\u7684\u83b7\u6279\uff0c\u4e0d\u4ec5\u5bf9\u60a3\u8005\u548c\u533b\u751f\u6765\u8bf4\u5177\u6709\u91cc\u7a0b\u7891\u7684\u610f\u4e49\uff0c\u8fd8\u5c06\u6380\u8d77\u4e00\u573a\u5236\u836f\u884c\u4e1a\u7684\u9769\u547d\uff0c\u662f\u8bfa\u8d1d\u5c14\u5956\u6210\u679c\u4ece\u6982\u5ff5\u8d70\u5411\u5b9e\u9645\u6cbb\u7597\u7528\u9014\u7684\u4e00\u4e2a\u5149\u8f89\u91cc\u7a0b\u7891\u3002\u800c\u6210\u529f\u4fc3\u4f7fRNAi\u4ece\u8bba\u6587\u8d70\u5411\u4e34\u5e8a\u5b9e\u9645\u5e94\u7528\u7684\u6210\u529f\u7684\u8f7d\u8fd0\u6280\u672f\u5e94\u7528\u2014\u2014\u7eb3\u7c73\u8102\u8d28\u7c92(lipid nanoparticle, LNP)\uff0c<strong>\u672c\u6587\u8bd5\u4ece\u5236\u5242\u7684\u89d2\u5ea6\uff0c\u6982\u8ff0LNP\u7ed9\u836f\u7cfb\u7edf\u7684\u8bbe\u8ba1\u53ca\u5236\u5907\u6280\u672f\u3001\u809d\u810f\u53ca\u5176\u5b83\u7ec4\u7ec7\u9776\u5411\u6027\u53ca\u672a\u6765\u524d\u666f\u7b49\u8fdb\u884c\u6982\u8ff0<\/strong>\u3002<\/span><\/p>\n<\/section>\n<section style=\"display: flex;justify-content: flex-end;\">\n<section style=\"width: 45px;padding-right: 5px;padding-left: 5px;margin-bottom: -11px;box-sizing: border-box;\"><img class=\"\"      style=\"width: 100%;background: none rgb(156, 218, 245);margin-left: 5px;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/09\/3-1537926998.png\" alt=\"LNP\uff1a\u4fc3\u8fdbRNAi\u7531\u7406\u8bba\u5230\u4e34\u5e8a\u5e94\u7528\" title=\"LNP\uff1a\u4fc3\u8fdbRNAi\u7531\u7406\u8bba\u5230\u4e34\u5e8a\u5e94\u7528\"  \/><\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section  class=\"_135editor\" style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<p><br  \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section  label=\"Powered by 135editor.com\" style=\"font-size:16px;font-family:\u5fae\u8f6f\u96c5\u9ed1;\">\n<section  label=\"Powered by 135editor.com\">\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;display: inline-block;box-sizing: border-box;\"  >\n<p style=\"margin-top: 8px;padding-right: 10px;font-weight: bold;line-height: 28px;max-width: 100%;color: rgb(0, 128, 255);min-height: 32px;border-bottom: 1.5px solid rgb(0, 128, 255);border-top-color: rgb(0, 128, 255);border-right-color: rgb(0, 128, 255);border-left-color: rgb(0, 128, 255);text-align: justify;\"><span class=\"autonum\"  placeholder=\"1\" style=\"color: rgb(255, 255, 255);display: block;float: left;line-height: 20px;margin-right: 8px;max-width: 100%;padding: 4px 10px;background-color: rgb(0, 128, 255);word-wrap: break-word !important;\" title=\"\">1<\/span><strong style=\"font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;\">RNAi\u7684\u6cbf\u9769<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\"><\/span><\/p>\n<p style=\"text-align: justify;line-height: 1.75em;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">RNA\u5e72\u6d89(RNA interference\uff0cRNAi) \u662f\u6b63\u5e38\u751f\u7269\u4f53\u5185\u6291\u5236\u7279\u5b9a\u57fa\u56e0\u8868\u8fbe\u7684\u4e00\u79cd\u73b0\u8c61\uff0c\u5b83\u662f\u6307\u5f53\u7ec6\u80de\u4e2d\u5bfc\u5165\u4e0e\u5185\u6e90\u6027mRNA\u7f16\u7801\u533a\u540c\u6e90\u7684\u53cc\u94feRNA (double stranded RNA\uff0cdsRNA )\u65f6\uff0c\u8be5mRNA\u53d1\u751f\u964d\u89e3\u800c\u5bfc\u81f4\u57fa\u56e0\u8868\u8fbe\u6c89\u9ed8\u7684\u73b0\u8c61\u3002\u8fd9\u79cd\u73b0\u8c61\u53d1\u751f\u5728\u8f6c\u5f55\u540e\u6c34\u5e73\uff0c\u53c8\u79f0\u4e3a\u8f6c\u5f55\u4e86\u540e\u57fa\u56e0\u6c89\u9ed8 (post transcriptional gene silencing\uff0cvrGs)<\/span><\/p>\n<p style=\"text-align: justify;line-height: 1.75em;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">&nbsp;<\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">2006\u5e74\u745e\u5178\u5361\u7f57\u6797\u65af\u5361\u533b\u5b66\u9662\u5ba3\u5e03\u5c06\u8bfa\u8d1d\u5c14\u751f\u7406\u5b66\u6216\u533b\u5b66\u5956\u6388\u4e88\u4e24\u540d\u7f8e\u56fd\u79d1\u5b66\u5bb6\u5b89\u5fb7\u9c81\u00b7\u6cd5\u5c14(Andrew Z Fire)\u548c\u514b\u96f7\u683c\u00b7\u6885\u6d1b(Craig Mello)\uff0c\u4ee5\u8868\u5f70\u4ed6\u4eec\u53d1\u73b0\u4e86\u201cRNA\u5e72\u6270\u673a\u5236\u2014\u2014\u53cc\u94feRNA\u5f15\u8d77\u57fa\u56e0\u6c89\u9ed8\u201d\u3002\u8bfa\u8d1d\u5c14\u5956\u8bc4\u5ba1\u59d4\u5458\u4f1a\u7684\u516c\u62a5\u4e2d\u79f0\uff1a\u201c\u4ed6\u4eec\u7684\u53d1\u73b0\u6f84\u6e05\u4e86\u8bb8\u591a\u4ee4\u4eba\u8ff7\u60d1\u548c\u77db\u76fe\u7684\u8bd5\u9a8c\u7ed3\u679c\uff0c\u63ed\u5f00\u4e86\u5927\u81ea\u7136\u63a7\u5236\u9057\u4f20\u4fe1\u606f\u4f20\u9012\u7684\u4e00\u79cd\u57fa\u672c\u673a\u5236\u3002\u4ed6\u4eec\u5f00\u521b\u4e86\u4e00\u4e2a\u5d2d\u65b0\u7684\u7814\u7a76\u9886\u57df\u201d\u3002<\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">&nbsp;<\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">\u53ef\u4ee5\u8bf4\uff0c<strong>\u8fd9\u9879\u7a81\u7834\u6027\u7684\u6280\u672f\uff0c\u6253\u5f00\u4e86\u4e00\u6247\u901a\u5f80\u65b0\u5929\u5730\u7684\u5927\u95e8<\/strong>\u3002\u8fd9\u4e00\u521b\u65b0\u7684\u5de5\u4f5c\u5f97\u5230\u4e86\u8bb8\u591a\u751f\u7269\u5b9e\u9a8c\u5ba4\u7684\u9752\u7750\uff0c\u4e5f\u52a0\u901f\u4e86\u751f\u7269\u5b66\u7684\u53d1\u5c55\u3002\u66f4\u91cd\u8981\u7684\u662f\uff0c\u5b83\u8ba9\u6211\u4eec\u770b\u5230\u4e86\u901a\u8fc7\u201c\u57fa\u56e0\u6c89\u9ed8\u201d\u6cbb\u7597\u75be\u75c5\u7684\u5e0c\u671b\u3002\u6b63\u5982\u8bfa\u8d1d\u5c14\u5956\u5b98\u65b9\u65b0\u95fb\u7a3f\u4e2d\u6240\u8bf4\u7684\u90a3\u6837\uff0c\u201cRNA\u5e72\u6270\u5df2\u7ecf\u5728\u57fa\u7840\u79d1\u7814\u4e2d\u5f97\u5230\u4e86\u5e7f\u6cdb\u7684\u5e94\u7528\uff0c\u80fd\u7528\u4ee5\u7814\u7a76\u57fa\u56e0\u7684\u529f\u80fd\u3002\u5b83\u8fd8\u6709\u671b\u5728\u672a\u6765\u5e26\u6765\u5168\u65b0\u7684\u7597\u6cd5\u3002\u201d<\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">&nbsp;<\/span><\/p>\n<h1 style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">\u65e9\u5728\u5728\u8bfa\u8d1d\u5c14\u5956\u9881\u53d1\u76845\u5e74\u524d\uff0c2001\u5e74\u4eba\u7c7b\u57fa\u56e0\u7ec4\u8349\u56fe\u7684\u6d4b\u5e8f\u5de5\u4f5c\u7684\u5b8c\u6210\uff0c\u8bb8\u591a\u7814\u7a76\u4eba\u5458\u5c31\u5f00\u59cb\u5728\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u91cc\u5c1d\u8bd5\u5e94\u7528RNAi\uff0c<strong>\u751f\u7269\u6280\u672f\u516c\u53f8\u5982\u96e8\u540e\u6625\u7b0b\u822c\u76f8\u7ee7\u95ee\u4e16\uff0c\u4e89\u5f53\u7b2c\u4e00\u6b3eRNAi\u65b0\u836f\u7684\u53d1\u660e\u4eba\u3002<\/strong><\/span><\/h1>\n<p><span style=\"font-size: 15px;color: rgb(0, 0, 0);\"><strong><br  \/><\/strong><\/span><\/p>\n<section  label=\"Powered by 135editor.com\" style=\"font-size:16px;font-family:\u5fae\u8f6f\u96c5\u9ed1;\">\n<section  label=\"Powered by 135editor.com\">\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\"  >\n<section style=\"width: 100%;\" >\n<section style=\"text-align:center;margin-bottom: -16px;\">\n<section class=\"135brush\"  style=\"display: inline-block;background: rgb(182, 228, 253);color: rgb(5, 115, 175);letter-spacing: 1.5px;line-height: 1.75em;padding: 2px 1em;box-sizing: border-box;\"><strong><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1, sans-serif;\">RNAi<\/span><\/strong><strong><span style=\"font-family: \u5fae\u8f6f\u96c5\u9ed1, sans-serif;\">\u6280\u672f\u53d1\u5c55\u7684\u5386\u7a0b<\/span><\/strong><\/section>\n<\/section>\n<p style=\"border-width: 1px;border-style: solid;border-color: rgb(182, 228, 253);border-radius: 10px;padding: 2.5em 1em 1em;line-height: 1.75em;\"><span style=\"font-size: 15px;\">1998\uff1a\u690d\u7269\u57fa\u56e0\u4e2d\u57fa\u56e0\u6c89\u9ed8\u73b0\u8c61\u7684\u53d1\u73b0 <br  \/> 2000\uff1a\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u4e2d\u57fa\u56e0\u6c89\u9ed8\u7684\u5b9e\u73b0 <br  \/> 2001\uff1a\u88ab\u300a\u79d1\u5b66\u300b\u8bc4\u4e3a\u5f53\u5e74\u5341\u5927\u79d1\u6280\u7a81\u7834\u4e4b\u4e00 <br  \/> 2003\uff1a\u52a8\u7269\u4f53\u5185\u89c2\u5bdf\u5230RNA\u5e72\u6270\u4f5c\u7528 <br  \/> 2004\uff1a\u5728\u6052\u6cb3\u7334\u4e0a\u7684SARS\u75c5\u6bd2\u7814\u7a76\u53d6\u5f97\u8fdb\u5c55 <br  \/> 2004\uff1aAcuity Pharmaceutical \u7b2c\u4e00\u4e2aRNA\u5e72\u6270\u836f\u7269\u7533\u8bf7IND<br  \/> 2004\uff1asiRNA Therapeutics \u7b2c\u4e00\u4e2aRNA\u5e72\u6270\u836f\u7269\u7533\u8bf7IND<br  \/> 2005\uff1a\u7b2c\u4e00\u4e2aRNA\u5e72\u6270\u836f\u7269\u8fdb\u5165\u4e00\u671f\u4e34\u5e8a\uff0c\u53d6\u5f97\u826f\u597d\u7684\u6548\u679c <br  \/> 2005\uff1a\u5316\u5b66\u4fee\u9970\u7684siRNA oligo \u4f53\u5185\u7cfb\u7edf\u7ed9\u836f\u53d6\u5f97\u7a81\u7834 <br  \/> <strong>2006<\/strong><strong>\uff1aAndrew Z. Fire\u548cCraig C. Mello\u56e0\u5728RNA\u5e72\u6270\u9886\u57df\u7684\u6210\u5c31\u83b7\u5f97\u8bfa\u8d1d\u5c14\u533b\u5b66\/\u751f\u7406\u5b66\u5956<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<section  class=\"_135editor\" style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<p><br  \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<section  label=\"Powered by 135editor.com\" style=\"font-size:16px;font-family:\u5fae\u8f6f\u96c5\u9ed1;\">\n<section  label=\"Powered by 135editor.com\">\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;display: inline-block;box-sizing: border-box;\"  >\n<p style=\"margin-top: 8px;padding-right: 10px;font-weight: bold;line-height: 28px;max-width: 100%;color: rgb(0, 128, 255);min-height: 32px;border-bottom: 1.5px solid rgb(0, 128, 255);border-top-color: rgb(0, 128, 255);border-right-color: rgb(0, 128, 255);border-left-color: rgb(0, 128, 255);text-align: justify;\"><span class=\"autonum\"  placeholder=\"1\" style=\"color: rgb(255, 255, 255);display: block;float: left;line-height: 20px;margin-right: 8px;max-width: 100%;padding: 4px 10px;background-color: rgb(0, 128, 255);word-wrap: break-word !important;\" title=\"\">2<\/span><strong style=\"font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;\">RNAi\u7814\u7a76\u7684\u56f0\u5883&nbsp;<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">\u57fa\u56e0\u6cbb\u7597\u65e9\u671f\u7814\u7a76\u65b9\u5411\u4fa7\u91cd\u4e8e\u901a\u8fc7\u4f20\u9012DNA\u6765\u8bf1\u5bfc\u76ee\u6807\u7ec6\u80de\u8868\u8fbe\u7279\u5b9a\u7684\u57fa\u56e0\u3002\u4f46pDNA\u5728\u5c0f\u9f20\u5168\u8840(\u4f53\u5916)\u4e2d\u7684\u534a\u8870\u671f\u4ec5\u4e3a10 min\uff0c\u9759\u8109\u6ce8\u5c04\u540e\u5728\u4f53\u5185\u7684\u534a\u8870\u671f\u751a\u81f3\u66f4\u77ed\uff0c\u540c\u65f6\u8fd8\u6709\u5feb\u901f\u7684\u809d\u79ef\u805a\u3002\u6240\u4ee5\u6838\u9178\u6cbb\u7597\u7684<strong>\u7b2c\u4e00\u4e2a\u4e3b\u8981\u969c\u788d<\/strong>\u662f\u8840\u6e05\u6838\u9178\u9176\u6d3b\u6027\u4f7f\u5f97pDNA\u5728\u8840\u6e05\u4e2d\u5feb\u901f\u7684\u6d88\u9664\u3002<strong>\u7b2c\u4e8c\u4e2a\u969c\u788d<\/strong>\u5c31\u662f\u514d\u75ab\u523a\u6fc0\uff0c\u8fd9\u79cd\u514d\u75ab\u53cd\u5e94\u53ef\u4ee5\u901a\u8fc7\u6838\u9178\u7684\u5316\u5b66\u4fee\u9970\u90e8\u5206\u89c4\u907f,\u5982mRNA\u548csiRNA\uff0c\u4f46\u4ece\u751f\u7269\u6765\u6e90\u7eaf\u5316\u7684pDNA(\u975e\u5168\u5408\u6210)\uff0c\u5e76\u4e0d\u9002\u5408\u8fd9\u79cd\u4fee\u9970\u3002\u5e76\u4e14\u5728\u751f\u7269\u7eaf\u5316\u8fc7\u7a0b\u4e2d\u4e5f\u6613\u53d7\u5230\u539f\u6750\u6599\u4e2d\u70ed\u539f(\u5185\u6bd2\u7d20)\u7684\u5f71\u54cd\u3002\u540e\u671f\u7684\u7814\u7a76\u4e5f\u66f4\u52a0\u96c6\u4e2d\u5728mRNA\u548csiRNA<\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">&nbsp;<\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">\u5373\u4f7f\u88ab\u7ecf\u8fc7\u5927\u91cf\u4fee\u9970\u7684\u6838\u9178\u8de8\u8fc7\u4e86\u524d\u4e24\u4e2a\u969c\u788d\uff0c\u5b83\u4eec\u4ecd\u7136\u53d7\u5230<strong>\u7b2c\u4e09\u79cd\u969c\u788d<\/strong>\u7684\u5f71\u54cd\u2014\u2014\u80be\u8fc7\u6ee4(\u5c0f\u4e8e6nm\u7684\u9897\u7c92\u7684\u9ad8\u6548\u8fc7\u6ee4)\u3002\u5bf9\u5be1\u6838\u82f7\u9178\u836f\u52a8\u5b66\u7684\u7814\u7a76\u8868\u660e\uff0c50%\u4ee5\u4e0a\u7684\u836f\u7269\u5728\u5c3f\u6db2\u4e2d\u6392\u6cc4\u3002\u4e3a\u4e86\u63d0\u9ad8\u6838\u9178\u836f\u7269\u7684\u751f\u7269\u5229\u7528\u5ea6\uff0c\u5df2\u7ecf\u8fdb\u884c\u4e86\u4e00\u4e9b\u5c1d\u8bd5\uff0c\u5982\u901a\u8fc7\u53f3\u65cb\u7cd6\u9150\u7684\u786b\u9178\u76d0\u7b49\u8d1f\u7535\u8377\u805a\u7269\u7b49\u3002\u867d\u7136\u53ef\u4ee5\u6539\u5584\u836f\u4ee3\u52a8\u529b\u5b66\u7279\u70b9\uff0c\u4f46\u4ecd\u7136\u4e0d\u80fd\u5b9e\u73b0\u6838\u9178\u5728\u9776\u90e8\u4f4d\u7d2f\u79ef\u3002\u5373\u4f7f\u5b9e\u73b0\u4e86\u6838\u9178\u7684\u9776\u5411\u95ee\u9898\uff0c\u7531\u4e8e\u6838\u9178\u7684\u5927\u5206\u5b50\u7ed3\u6784(\u5e73\u5747\u5206\u5b50\u91cf\u4e3aMW 13 kD)\u3001\u9ad8\u8d1f\u7535\u8377\u7684\u7269\u7406\u5316\u5b66\u7684\u7279\u70b9\u4f7f\u5176\u65e0\u6cd5\u7a7f\u900f\u7ec6\u80de\u819c\uff0c\u88ab\u7ec6\u80de\u5185\u5316\u540e\u6613\u88ab\u7ec6\u80de\u5185\u6eb6\u9176\u4f53\u964d\u89e3\u7684\u95ee\u9898\u4e5f\u4f9d\u7136\u5b58\u5728\u3002<\/span><\/p>\n<section  label=\"Powered by 135editor.com\" style=\"font-size:16px;font-family:\u5fae\u8f6f\u96c5\u9ed1;\">\n<section  label=\"Powered by 135editor.com\">\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\"  >\n<section style=\"width: 100%;padding-top: 20px;padding-bottom: 20px;box-sizing: border-box;\" >\n<section style=\"width: 100%;\" >\n<section style=\"font-size: 15px;padding-top: 10px;padding-bottom: 10px;text-align: justify;line-height: 25px;letter-spacing: 1px;color: rgb(194, 201, 42);box-sizing: border-box;\" class=\"135brush\">\n<p style=\"text-align: center;\"><strong><span style=\"font-family: Helvetica, Arial, sans-serif;text-align: justify;color: rgb(79, 97, 40);\">\u6f6e\u6c34\u9000\u540e\uff0c\u88f8\u9732\u51fa\u6765\u662f\u77f3\u5934\uff0c<\/span><\/strong><\/p>\n<p style=\"text-align: center;\"><strong><span style=\"font-family: Helvetica, Arial, sans-serif;text-align: justify;color: rgb(79, 97, 40);\">\u4e5f\u662f\u6478\u7740\u8fc7\u6cb3\u7684\u77f3\u5934<\/span><\/strong><\/p>\n<\/section>\n<\/section>\n<section>\n<section style=\"width: 100%;height: 6px;background: #c2c92a;margin-bottom: -18px;overflow: hidden;box-shadow: #999999 -1px 1px 5px 0px;color: #ffffff;\" ><\/section>\n<section style=\"width: 25px;height: 25px;border-radius: 100%;background: rgb(255, 255, 255);margin-right: auto;margin-left: auto;border-width: 5px;border-style: solid;border-color: rgb(194, 201, 42);overflow: hidden;box-sizing: border-box;\"><\/section>\n<section style=\"width: 100%;height: 10px;background: rgb(255, 255, 255);margin-top: -32px;padding-bottom: 20px;box-sizing: border-box;\" ><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section  class=\"_135editor\" style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section  label=\"Powered by 135editor.com\" style=\"font-size:16px;font-family:\u5fae\u8f6f\u96c5\u9ed1;\">\n<section  label=\"Powered by 135editor.com\">\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"margin: 10px;box-sizing: border-box;\">\n<section style=\"width: 12px;height: 12px;float: left;padding: 1px;background-color: #ffffff;box-sizing: border-box;\">\n<section style=\"width: 6px;height: 6px;border-width: 1px;border-style: solid;border-color: #e1c129;border-radius: 50%;box-sizing: border-box;\"><\/section>\n<\/section>\n<section style=\"width: 12px;height: 12px;float: right;padding: 1px;background-color: #ffffff;box-sizing: border-box;\">\n<section style=\"width: 6px;height: 6px;border-width: 1px;border-style: solid;border-color: #e1c129;border-radius: 50%;margin-left: auto;box-sizing: border-box;\"><\/section>\n<\/section>\n<section style=\"clear: both;box-sizing: border-box;\"><\/section>\n<section style=\"margin-top: -12px;margin-bottom: -12px;box-sizing: border-box;\">\n<section style=\"border-width: 1px;border-style: dotted;border-color: #d2d2d2;box-sizing: border-box;\">\n<section>\n<section style=\"padding: 1em 0.8em;box-sizing: border-box;\">\n<section style=\"text-align: center;font-size: 14px;color:#3f3f3f;line-height:1.75em;letter-spacing: 1.5px;text-align:justify;\" class=\"135brush\" >\n<p style=\"font-family: Helvetica, Arial, sans-serif;font-size: medium;line-height: 1.75em;\"><strong><span style=\"font-size: 15px;\">\u6838\u9178\u7c7b\u836f\u7269\u4f53\u5185\u5e94\u7528\u9700\u8981\u89e3\u51b3\u4ee5\u4e0b\u95ee\u9898:<\/span><\/strong><\/p>\n<ul class=\"list-paddingleft-2\" style=\"\">\n<li>\n<p style=\"line-height: 1.75em;\"><span style=\"font-size: 15px;\">\u5408\u7406\u7684\u8bbe\u8ba1\u57fa\u56e0\u5e8f\u5217\u53ca\u907f\u514d\u8131\u9776\u6548\u5e94<\/span><\/p>\n<\/li>\n<li>\n<p style=\"line-height: 1.75em;\"><span style=\"font-size: 15px;\">\u907f\u514d\u8840\u6db2\u53ca\u7ec6\u80de\u5185\u6838\u82f7\u9176\u7684\u964d\u89e3\u53ca\u514d\u75ab\u539f\u6027<\/span><\/p>\n<\/li>\n<li>\n<p style=\"line-height: 1.75em;\"><span style=\"font-size: 15px;\">\u7ed9\u836f\u7cfb\u7edf\u9012\u9001siRNA\u5206\u5b50\u8fdb\u5165\u9776\u7ec6\u80de\u5185<\/span><\/p>\n<\/li>\n<li>\n<p style=\"line-height: 1.75em;\"><span style=\"font-size: 15px;\">\u7ec6\u80de\u5185\u542b\u4f53\u9003\u9038\u8fdb\u5165\u80de\u6d46\u5e76\u5b9e\u73b0RNAi<\/span><\/p>\n<\/li>\n<\/ul>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"width: 12px;height: 12px;float: left;padding-top: 5px;padding-left: 1px;background-color: #ffffff;box-sizing: border-box;\">\n<section style=\"width: 6px;height: 6px;border-width: 1px;border-style: solid;border-color: #e1c129;border-radius: 50%;box-sizing: border-box;\"><\/section>\n<\/section>\n<section style=\"width: 12px;height: 12px;float: right;padding-right: 1px;padding-top: 5px;background-color: #ffffff;box-sizing: border-box;\">\n<section style=\"width: 6px;height: 6px;border-width: 1px;border-style: solid;border-color: #e1c129;margin-left: auto;border-radius: 50%;box-sizing: border-box;\"><\/section>\n<\/section>\n<section style=\"clear: both;box-sizing: border-box;\"><\/section>\n<\/section>\n<\/section>\n<section  class=\"_135editor\" style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<p><br  \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">\u800c\u5982\u524d\u6240\u8ff0\u901a\u8fc7\u751f\u7269\u4fe1\u606f\u5b66\u53ef\u6709\u6548\u8bbe\u8ba1siRNA\u5e8f\u5217\uff0c\u907f\u514d\u8131\u9776\u6548\u5e94\uff1b\u901a\u8fc7\u6838\u9178\u5316\u5b66\u4fee\u9970\u7684siRNA\u53ef\u907f\u514d\u6838\u82f7\u9176\u964d\u89e3\uff0c\u964d\u4f4e\u514d\u75ab\u539f\u6027\u3002<strong>\u4f46\u76ee\u524d\u5236\u7ea6siRNA\u5206\u5b50\u4e34\u5e8a\u5e94\u7528\u7684\u74f6\u9888\u95ee\u9898\u662f\u7ed9\u836f\u7cfb\u7edf\u53ca\u5185\u6db5\u4f53\u9003\u9038\u3002<\/strong><\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\"><br  \/><\/span><\/p>\n<section  label=\"Powered by 135editor.com\" style=\"font-size:16px;font-family:\u5fae\u8f6f\u96c5\u9ed1;\">\n<section  label=\"Powered by 135editor.com\">\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;display: inline-block;box-sizing: border-box;\"  >\n<p style=\"margin-top: 8px;padding-right: 10px;font-weight: bold;line-height: 28px;max-width: 100%;color: rgb(0, 128, 255);min-height: 32px;border-bottom: 1.5px solid rgb(0, 128, 255);border-top-color: rgb(0, 128, 255);border-right-color: rgb(0, 128, 255);border-left-color: rgb(0, 128, 255);text-align: justify;\"><span class=\"autonum\"  placeholder=\"1\" style=\"color: rgb(255, 255, 255);display: block;float: left;line-height: 20px;margin-right: 8px;max-width: 100%;padding: 4px 10px;background-color: rgb(0, 128, 255);word-wrap: break-word !important;\" title=\"\">3<\/span><strong style=\"font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;\">\u7528\u4e8e\u9012\u9001\u6838\u9178\u7684LNP\u7684\u8bbe\u8ba1<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\"><\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">\u8102\u8d28\u7eb3\u7c73\u7c92\u5b50(lipidnanoparticle,LNP)\u8f7d\u8fd0\u5c0f\u5206\u5b50\u836f\u7269\u7684\u5236\u5242\u6280\u672f\u5df2\u7ecf\u76f8\u5bf9\u6210\u719f\uff0c\u76ee\u524d\u5168\u4e16\u754c\u5df2\u6279\u51c69\u79cd\u8102\u8d28\u4f53\u836f\u7269\u5236\u5242\u7528\u4e8e\u9759\u8109\u7ed9\u836f\uff0c\u5b83\u5177\u6709\u9002\u5b9c\u7684\u7c92\u5f84(100nm\u76f4\u5f84\u6216\u66f4\u5c0f)\uff0c\u9ad8\u5c01\u88c5\u6548\u7387\uff0c\u5236\u5907\u5de5\u827a\u7a33\u5b9a\u3001\u8868\u9762\u7535\u8377\u4f4e\u7684\u7279\u70b9(\u4f4e\u8868\u9762\u7535\u8377\u53ef\u4ee5\u51cf\u5c11\u4e0e\u8840\u6e05\u86cb\u767d\u7684\u76f8\u4e92\u4f5c\u7528)\u3002\u5f53\u901a\u8fc7\u4e0d\u540c\u7684\u8f7d\u836f\u7b56\u7565\u548c\u5236\u5907\u6280\u672f\u914d\u5408\uff0cLNP\u8f7d\u836f\u7cfb\u7edf\u53ef\u4ee5\u5b9e\u73b0\u6838\u9178\u805a\u5408\u7269\u7684\u6709\u6548\u805a\u96c6(&gt; 80\uff05)\u548c\u9012\u9001\uff0c\u4e3a\u4e86\u5b9e\u73b0\u5e26\u8d1f\u7535\u8377\u7684\u6838\u9178\u7684\u6709\u6548\u5c01\u88c5\uff0c\u5219\u9700\u8981\u5f15\u5165\u5bf9RNA\u6216DNA\u6709\u5f88\u5f3a\u9759\u7535\u7ed3\u5408\u529b\u7684\u9633\u79bb\u5b50\u8102\u8d28\uff0c\u8fd9\u7c7b\u9633\u79bb\u5b50\u8102\u8d28\u7814\u53d1\u7ecf\u5386\u4e86\u5982\u4e0b\u6240\u8ff0\u7ecf\u5386\u4e86\u76f8\u5f53\u957f\u7684\u8fc7\u7a0b\u3002<\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">&nbsp;<\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"color: rgb(0, 128, 255);\"><strong><span style=\"font-size: 15px;\">3.1 \u9633\u79bb\u5b50\u8102\u8d28\u548c\u201clipoplexes\u201d<\/span><\/strong><\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">\u65e9\u671f\u5f00\u53d1LNP\u7cfb\u7edf\u7684\u4e3b\u8981\u52aa\u529b\u65b9\u5411\u662f\u5229\u7528\u4e2d\u6027(\u4e24\u6027\u79bb\u5b50)\u8102\u7c7b\u7684\u88ab\u52a8\u5f0f\u5305\u5c01\u6cd5\uff0c\u5982\u57fa\u4e8eDOTMA\u7684\u7814\u53d1\u7684\u590d\u5408\u7269\uff0c\u5c06DOTMA\u4e0e\u4e00\u79cd\u201c\u8f85\u52a9\u6027\u8102\u201d\u4e8c\u6cb9\u57fa\u78f7\u8102\u9170\u4e59\u9187\u80fa(DOPE)\u7ed3\u5408\u5728\u4e00\u8d77\u7684\u201clipoplexes\u201d\uff0c\u8fd9\u79cd\u65b9\u6cd5\u5229\u7528\u9759\u7535\u7535\u8377\u5438\u5f15\u7684\u65b9\u5f0f\u6765\u5b9e\u73b0\u5305\u5c01\u3002\u4f46\u6709\u5305\u5c01\u6548\u7387\u8f83(&lt;40\uff05)\u3001\u8f6c\u67d3\u6548\u529b\u6709\u9650\u7684\uff0c\u70ed\u529b\u5b66\u4e0d\u7a33\u5b9a\u7684\u95ee\u9898\u7f3a\u70b9\uff0c\u8fd8\u6709\u6eb6\u8840\u3001\u514d\u75ab\u539f\u6027\u3002\u548c\u5176\u4ed6\u6bd2\u6027\u7684\u7f3a\u70b9\u9650\u5236\u4e86\u5b83\u5728\u6d3b\u4f53\u5b9e\u9a8c\u7684\u7814\u7a76\u3002<\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">&nbsp;<\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"color: rgb(0, 128, 255);\"><strong><span style=\"font-size: 15px;\">3.2 \u9633\u79bb\u5b50\u8102\u8d28\u548cLNPs<\/span><\/strong><\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">\u7b2c\u4e00\u4ee3\u8f7d\u8fd0\u9057\u4f20\u836f\u7269(pDNA) \u7684LNP\u5236\u5907\u65b9\u6848\u662f\u91c7\u7528\u9633\u79bb\u5b50\u8102\u8d28\u901a\u8fc7\u6d17\u6da4-\u900f\u6790\u7684\u6280\u672f\u5236\u5907\u3002\u8be5\u65b9\u6cd5\u662f\u5c06pDNA\u4e0e\u9633\u79bb\u5b50\u8102\u8d28(DODAC)\u540cOGP\u7b49\u8868\u9762\u6d3b\u6027\u5242\u6df7\u5408\uff0c\u7136\u540e\u5728OGP\u6eb6\u6db2\u4e2d\u52a0\u5165\u542b\u5375\u78f7\u8102\u548cPEG-\u8102\u7684\u6eb6\u6db2\u3002\u6700\u540e\u7684\u6df7\u5408\u7269\u88ab\u900f\u679036-48\u5c0f\u65f6\uff0c\u53bb\u9664\u6d17\u6da4\u5242\u5e76\u5f62\u6210\u7eb3\u7c73\u9897\u7c92\u3002\u5f53\u9633\u79bb\u5b50\u8102\u8d28\u542b\u91cf\u4e3a6~8 mol%\u65f6\uff0c\u5305\u5c01\u7387\u4e3a30%\uff0c\u8fdb\u4e00\u6b65\u7528DOPE\u4ee3\u66ff\u5375\u78f7\u8102\u65f6\uff0c\u5305\u5c01\u7387\u53ef\u4ee5\u8fbe\u523070%\u3002\u8fd9\u4e9b\u9897\u7c92\u88ab\u79f0\u4e3aSPLP (stabilizedplasmid-lipid-particles)\uff0c\u5728\u4f4e\u6e29\u900f\u5c04\u7535\u955c\u4e0b\u5448\u5355\u7247\u72b6\u7ed3\u6784\uff0c\u7c92\u5f84\u5206\u5e03\u572870 nm\u5de6\u53f3\u3002SPLP\u5177\u6709\u4f53\u5185\u957f\u5faa\u73af\u7279\u6027\uff0c\u5728\u5c0f\u9f20\u4e2dt1\/2\u4e3a 7\u5c0f\u65f6\u3002\u8fd9\u4f7f\u5f972.5\uff05\u7684\u6ce8\u5c04\u5242\u91cf\u572824\u5c0f\u65f6\u5185\u5728\u8fdc\u7aef\u80bf\u7624\u5904\u79ef\u7d2f\uff0c\u540c\u65f6\u5728\u809d\u810f\u548c\u813e\u810f\u4e2d\u6c34\u5e73\u975e\u5e38\u4f4e\u3002\u91cd\u8981\u7684\u662f\uff0c\u4e0eLipoplexes\u76f8\u6bd4\uff0cSPLP\u7684\u809d\u6bd2\u6027\u660e\u663e\u964d\u4f4e\uff0c\u572830 mg DNA\u7684\u5242\u91cf\u4e0bLipoplexes\u53ef\u4f7f\u8840\u6e05\u4e2d\u7684\u4e19\u6c28\u9178\u8f6c\u6c28\u9176(ALT)\u548c\u5929\u51ac\u6c28\u9178\u8f6c\u6c28\u9176(AST)\u7684\u6c34\u5e73\u589e\u52a0100\u500d\uff0c\u800c175 mg DNA\u7684SPLP\u5728\u8840\u6e05\u4e2d\u68c0\u6d4b\u5230ALT\u548cAST\u7684\u5904\u5728\u57fa\u7ebf\u6c34\u5e73\u3002\u7136\u800c\uff0c\u7531\u4e8e\u5305\u5c01\u7387\u4f4e\u3001\u6548\u529b\u6709\u9650\u4ee5\u53ca\u6d17\u6da4\u5242\u900f\u6790\u5236\u9020\u6280\u672f\u662f\u5355\u4e00\u4e14\u4e0d\u53ef\u6269\u5c55\u7684\u539f\u56e0\uff0c\u963b\u788d\u4e86SPLP\u65b9\u6cd5\u7684\u8fdb\u4e00\u6b65\u524d\u8fdb\u3002<\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><strong><span style=\"font-size: 15px;color: rgb(0, 0, 0);\"><br  \/><\/span><\/strong><\/p>\n<h2 style=\"line-height: 1.75em;text-align: justify;\"><span style=\"color: rgb(0, 128, 255);\"><strong><span style=\"font-size: 15px;\">3.3 \u79bb\u5b50\u5316\u7684\u9633\u79bb\u5b50\u8102\u8d28\u548c\u4e59\u9187\u8f7d\u8377<\/span><\/strong><\/span><\/h2>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">\u6d17\u6da4-\u900f\u6790\u5236\u5907\u8fc7\u7a0b\u867d\u7136\u53ef\u4ee5\u751f\u4ea7\u5747\u5300\u7684LNP\uff0c\u4f46\u5236\u5907\u6548\u7387\u4f4e\u4e0b\u3002\u56e0\u6b64\uff0c\u4e59\u9187\u8377\u8f7d\u5de5\u827a\u53ef\u4ee5\u4e0e\u53ef\u7535\u79bb\u7684\u9633\u79bb\u5b50\u8102\u8d28\u7ed3\u5408\u4f7f\u7528\u3002\u6700\u521d\u7684\u65b9\u6cd5\u662f\u7528\u4e59\u9187\u6eb6\u89e3\u8102\u8d28\uff0c\u5e76\u5c06\u8be5\u6eb6\u6db2\u52a0\u5165\u5230pH4\u7f13\u51b2\u6db2\u4e2d\u7684\u53cd\u4e49\u5be1\u6838\u82f7\u9178(ASO)\u6c34\u6eb6\u6db2\u4e2d\u3002\u7136\u540e\u5c06\u4ea7\u751f\u7684\u7c92\u5b50\u6324\u51fa\uff0c\u5f97\u5230\u5747\u76f8\u7684\u7c92\u5b50\u7fa4\u4e3a100 nm\u7684LNP\uff0c\u7136\u540e\u900f\u6790\u53bb\u9664\u6eb6\u5242\uff0c\u4e2d\u548cpH\u503c\u3002\u8be5\u5de5\u827a\u5305\u5c01\u7387\u53ef\u4ee5\u8fbe\u523080%\u3002\u968f\u540e\u5f00\u53d1\u4e86\u4e00\u79cd\u5728T\u578b\u7ba1\u4e2d\u6df7\u5408\u7684\u65b9\u6cd5\uff1a\u5148\u5c06\u8102\u8d28\u6750\u6599(\u5305\u62ec\u9633\u79bb\u5b50\u8102\u53ca\u805a\u4e59\u4e8c\u9187\u8102(PEG\u8102))\u6eb6\u89e3\u4e8e\u4e59\u9187\u4e2d\uff0c\u4e0e\u6838\u9178\u6c34\u6eb6\u6db2(pH4)\u8fdb\u884c\u6df7\u5408\uff0c\u540c\u6837\u53ef\u5f97\u5230\u76f4\u7ecf&lt;100nm\u7684LNP\u7cfb\u7edf\u3002 \u540e\u6765\u53c8\u5f00\u53d1\u4e86\u66f4\u5feb\u901f\u53ca\u53ef\u63a7\u7684\u5fae\u6d41\u4f53(microfluidic)\u6df7\u5408\u6280\u672f\uff0c\u5f97\u5230\u4e86\u8d28\u91cf\u66f4\u597d\u7684LNP\u7cfb\u7edf\u3002\u6240\u5f97\u7c92\u5b50\u5bf9ASO\u548cpDNA\u7cfb\u7edf\u7684\u5305\u5c01\u7387\u5206\u522b\u9ad8\u8fbe90%\u548c70%\u3002<\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\"><br  \/><\/span><\/p>\n<section  label=\"Powered by 135editor.com\" style=\"font-size:16px;font-family:\u5fae\u8f6f\u96c5\u9ed1;\">\n<section  label=\"Powered by 135editor.com\">\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;display: inline-block;box-sizing: border-box;\"  >\n<p style=\"margin-top: 8px;padding-right: 10px;font-weight: bold;line-height: 28px;max-width: 100%;color: rgb(0, 128, 255);min-height: 32px;border-bottom: 1.5px solid rgb(0, 128, 255);border-top-color: rgb(0, 128, 255);border-right-color: rgb(0, 128, 255);border-left-color: rgb(0, 128, 255);text-align: justify;\"><span class=\"autonum\"  placeholder=\"1\" style=\"color: rgb(255, 255, 255);display: block;float: left;line-height: 20px;margin-right: 8px;max-width: 100%;padding: 4px 10px;background-color: rgb(0, 128, 255);word-wrap: break-word !important;\" title=\"\">4<\/span><strong style=\"font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;\">Onpattro (patisiran)\u5236\u5242\u6982\u8981<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\"><\/span><\/p>\n<h1 style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">Onpattro\u662f\u4e00\u79cd\u8102\u8d28\u590d\u5408\u7269\u6ce8\u5c04\u6db2<\/span><span style=\"font-size: 15px;color: rgb(0, 122, 170);\">(\u88681)<\/span><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">\uff0c\u5c06siRNA\u5305\u88f9\u5728\u8102\u8d28\u7eb3\u7c73\u9897\u7c92(LNP)\u4e2d\uff0c\u9759\u8109\u8f93\u6ce8\u540e\u836f\u7269\u76f4\u63a5\u9012\u9001\u81f3\u809d\u810f\u7ec6\u80de\u5185\uff0c\u6c89\u9ed8hATTR mRNA\u7684\u8868\u8fbe\uff0c\u51cf\u5c11\u4ea7\u751fhTTR\u86cb\u767d\uff0c\u9010\u6e10\u51cf\u5c11\u5468\u56f4\u795e\u7ecf\u4e2d\u6dc0\u7c89\u6837\u6c89\u79ef\u7269(hTTR)\u7684\u79ef\u7d2f\uff0c\u6700\u7ec8\u8fbe\u5230\u6cbb\u7597\u75be\u75c5\u7684\u76ee\u7684\u3002<\/span><\/h1>\n<p style=\"line-height: normal;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\"><br  \/><\/span><\/p>\n<p style=\"text-align: center;\"><img class=\"\"      style=\"\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/09\/1-1537926999.jpg\" alt=\"LNP\uff1a\u4fc3\u8fdbRNAi\u7531\u7406\u8bba\u5230\u4e34\u5e8a\u5e94\u7528\" title=\"LNP\uff1a\u4fc3\u8fdbRNAi\u7531\u7406\u8bba\u5230\u4e34\u5e8a\u5e94\u7528\"  \/><\/p>\n<p style=\"text-align: center;line-height: normal;\"><br  \/><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">\u5728\u514b\u670d\u57fa\u56e0\u4f20\u9012\u969c\u788d\u4e2dAlnylam Pharmaceuticals\u7684\u7ed9\u51fa\u7684\u8102\u8d28\u7eb3\u7c73\u7c92(LNP)\u65b9\u6848\u4e2d\u4e00\u9879\u5173\u952e\u6280\u672f\u5c31\u662f\u5728LNP-siRNA\u7cfb\u7edf\u4e2d\u63ba\u5165\u4e86\u4e00\u79cd\u7ecf\u8fc7\u4f18\u5316\u7684\u79bb\u5b50\u5316\u9633\u79bb\u5b50\u8102\u8d28(DLIN-MC3-DMA\uff0c\u7b80\u79f0MC3)\u3002\u5728LNP-siRNA\u7cfb\u7edf\u4e2d\u63ba\u5165MC3\u53ef\u5e26\u6765\u9ad8\u6548\u7684\u57fa\u56e0\u6c89\u9ed8\u6548\u679c\uff0c\u5728\u5c0f\u9f20\u6a21\u578b\u4e2d\uff0c\u5728\u4f4e\u5242\u91cf\u6c34\u5e73(5\u03bcg siRNA\/\u5c0f\u9f20 kg)\u4e0b\u53ef\u6709\u6548\u5730\u6c89\u9ed8\u5c0f\u9f20\u809d\u810f\u4e2d\u7684\u57fa\u56e0\u3002<\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">&nbsp;<\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">\u91c7\u7528\u79bb\u5b50\u5316\u9633\u79bb\u5b50\u8102\u8d28\u7684\u6700\u521d\u539f\u56e0\u662f\u4e3a\u4e86\u5b9e\u73b0\u5728\u4f4epH\u6761\u4ef6\u4e0b\u5bf9\u5e26\u8d1f\u7535\u805a\u5408\u7269\u7684\u6709\u6548\u5305\u5c01\uff0c\u5e76\u4e14\u5728pH7.4\u65f6\u4e5f\u663e\u793a\u51fa\u76f8\u5bf9\u65e0\u7535\u8377\u7684\u8868\u9762\u3002\u7136\u800c\uff0c\u7ecf\u9759\u8109\u7ed9\u836f\u7684LNP-siRNA\u7cfb\u7edf\u5bf9\u809d\u7ec6\u80de\u57fa\u56e0\u6c89\u9ed8\u7814\u7a76\u53d1\u73b0\u9009\u7528\u7684\u79bb\u5b50\u5316\u9633\u79bb\u5b50\u8102\u8d28\u81f3\u5173\u91cd\u8981\u3002\u5728\u8fdb\u4e00\u6b65\u7684\u8fdb\u884c\u5168\u9762\u7684\u8102\u8d28\u7b5b\u9009\u8fc7\u7a0b\u4e2d\uff0c\u4e00\u4e2a\u91cd\u8981\u7684\u539f\u5219\u5c31\u662f\uff0c\u53ef\u79bb\u5b50\u5316\u9633\u79bb\u5b50\u8102\u8d28\u5fc5\u987b\u6709\u5408\u9002\u7684pKa\uff0c\u53ea\u6709\u8db3\u591f\u9ad8\u7684pKa\u7684LNP\u5728\u5185\u6db5\u4f53\u9178\u6027pH\u503c\u4e0b\uff0c\u9633\u79bb\u5b50\u8102\u8d28\u5c31\u80fd\u4e0e\u5185\u6e90\u6027\u5185\u8d28\u9634\u79bb\u5b50\u8102\u8d28\u7ed3\u5408\uff0c\u5f62\u6210\u4fc3\u8fdb\u819c\u6eb6\u89e3\u7684\u975e\u53cc\u5c42\u7ed3\u6784\uff0c\u4ece\u800c\u4ece\u7ec6\u80de\u5185\u7684\u91ca\u653e\uff0c\u4f46\u8fc7\u9ad8\u7684pKa\u4f7f\u5f97LNP\u5728\u751f\u7406pH\u503c\u4e0b\u5177\u6709\u9ad8\u7684\u8868\u9762\u6b63\u7535\u8377\uff0c\u53ef\u4ee5\u5438\u9644\u8840\u6e05\u86cb\u767d\uff0c\u5e76\u8fc5\u901f\u4ece\u5faa\u73af\u4e2d\u6e05\u9664\u3002<\/span><\/p>\n<p style=\"text-align: justify;line-height: normal;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">&nbsp;<\/span><\/p>\n<section  label=\"Powered by 135editor.com\" style=\"font-size:16px;font-family:\u5fae\u8f6f\u96c5\u9ed1;\">\n<section  label=\"Powered by 135editor.com\">\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"text-align:center;\">\n<section style=\"display:inline-block;width:60px;\"><img class=\"_135editor\"     style=\"width: 60px;border-width: 0px;border-style: none;border-color: initial;\" title=\"LNP\uff1a\u4fc3\u8fdbRNAi\u7531\u7406\u8bba\u5230\u4e34\u5e8a\u5e94\u7528\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/09\/6-1537926999.png\" alt=\"LNP\uff1a\u4fc3\u8fdbRNAi\u7531\u7406\u8bba\u5230\u4e34\u5e8a\u5e94\u7528\"  \/><\/section>\n<\/section>\n<\/section>\n<section  class=\"_135editor\" style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<p style=\"line-height: normal;\"><br  \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">\u5728\u4f53\u5185\u809d\u810f\u9996\u6b21\u53d1\u73b0siRNA\u5177\u6709\u57fa\u56e0\u6c89\u9ed8\u6d3b\u6027\u7684\u7814\u7a76\u4f7f\u7528\u7684\u662f\u79bb\u5b50\u578b\u9633\u79bb\u5b50\u81021\uff0c2-\u4e8c\u7f9f\u57fa-3-\u4e8c\u7532\u57fa\u6c28\u57fa\u4e19\u70f7(DLinDMA\uff0c\u4e00\u79cd\u542b\u6709\u4e9a\u6cb9\u9178\u9170\u57fa\u94fe\u7684DODAP\u919a\u7c7b\u4f3c\u7269)\u5236\u5907LNP\uff0c\u968f\u540e\u53d1\u73b0LNP\u7684\u529f\u80fd\u6d3b\u6027\u4e0e\u6240\u7528\u9633\u79bb\u5b50\u8102\u79cd\u7c7b\u7279\u522b\u76f8\u5173\uff0c\u8fd9\u5f15\u8d77\u5e7f\u6cdb\u5408\u6210\u591a\u79cd\u8102\u7c7b\u7269\u8d28\u7684\u7814\u7a76\u548c\u8bc4\u4ef7\uff0c\u53d1\u73b0DLIN-KC2-DMA (KC2)\u4f5c\u4e3a\u79bb\u5b50\u5316\u7684\u9633\u79bb\u5b50\u8102\u8d28\uff0c\u6548\u679c\u4f18\u4e8e\u4e0eDLIDEMA\uff0c\u800cMC3-LNP\u7684ED 50\u8fdb\u4e00\u6b65\u964d\u4f4e\u4e14\u6bd2\u6027\u65e0\u589e\u52a0\uff0c\u5177\u5907\u4e86\u4e34\u5e8a\u5e94\u7528\u7684\u53ef\u80fd\u3002<\/span><br  \/><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">&nbsp;<\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><strong><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">\u8fd9\u79cd\u540d\u4e3a\u4e8c\u6cb9\u7532\u57fa-4-\u4e8c\u7532\u57fa\u6c28\u57fa\u4e01\u9178\u916f(DLin-MC3-DMA\uff0c\u901a\u5e38\u7f29\u5199\u4e3aMC3)\u7684\u8102\uff0c\u73b0\u5728\u6210\u4e3a\u5236\u5907\u809d\u810f\u9776\u5411siRNA\/LNP\u7cfb\u7edf\u7528\u8102\u7c7b\u6750\u6599\u7684\u201c\u91d1\u6807\u51c6\u201d\u3002<\/span><\/strong><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">\u4e0e\u7528DLinDMA\u76f8\u6bd4\uff0c\u7528MC3\u5236\u5907siRNA\/LNP\u7ed9\u836f\u7cfb\u7edf\u540e\uff0c\u809d\u810f\u7ec4\u7ec7\u7ec6\u80de\u5185\u7684\u57fa\u56e0\u6c89\u9ed8\u6d3b\u6027\u663e\u8457\u63d0\u9ad8\u4e09\u4e2a\u6570\u91cf\u7ea7\uff0c\u800c\u9633\u79bb\u5b50\u8102\u7ed3\u6784\u7684\u53d8\u5316\u5bf9\u6d3b\u6027\u7684\u5f71\u54cd\u76f8\u5bf9\u8f83\u5c0f\u3002\u8fdb\u4e00\u6b65\u7814\u7a76\u53d1\u73b0\uff0c\u542b\u6709\u79bb\u5b50\u578b\u9633\u79bb\u5b50\u8102\u8d28\u7684LNPsiRNA\u7cfb\u7edf\u5c31\u50cf\u4e73\u7cdc\u7c92\u8102\u86cb\u767d(chylomicrons)\u4e00\u6837\uff0c\u901a\u8fc7\u5438\u9644\u5185\u6e90\u6027\u7684ApoE\uff0c\u4ece\u800c\u89e6\u53d1\u809d\u7ec6\u80de\u8868\u9762\u7684\u8f7d\u8102\u86cb\u767d(ApoE)\u53d7\u4f53\u6444\u53d6\uff0c\u8fbe\u5230\u8fdb\u5165\u809d\u810f\u7ec6\u80de\u5185\u7684\u76ee\u7684\u3002<\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">&nbsp;<\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">\u6240\u4ee5Alnylam Pharmaceuticals\u7684\u91c7\u7528MC3\u6784\u5efaLNP-siRNA\uff0c\u7528\u4ee5\u9759\u9ed8\u7531TTR\u57fa\u56e0\u7a81\u53d8\u4ee5\u6cbb\u7597\u9057\u4f20\u6027\u8fd0\u7532\u72b6\u817a\u7d20\u86cb\u767d(TTR)\u4ecb\u5bfc\u6dc0\u7c89\u6837\u53d8\u5f15\u8d77\u7684\u81f4\u547d\u75be\u75c5\u3002\u7814\u7a76\u53d1\u73b0\u8fd9\u4e00\u7a81\u53d8\u5bf9\u542bMC3\u7684LNP-siRNA\u5f15\u8d77\u7684\u57fa\u56e0\u6c89\u9ed8\u6781\u4e3a\u654f\u611f\u3002\u5728\u4e00\u671f\u4e34\u5e8a\u4e2d\u57280.5 mg siRNA\/kg\u65f6\u4eba\u4f53\u8010\u53d7\u6781\u597d\uff0c\u800c\u4e8c\u671f\u4e34\u5e8a\u5b9e\u9a8c\u4e2d0.3 mg siRNA\/kg\u5c31\u53ef\u4ee5\u6536\u5230\u5f88\u597d\u7684\u57fa\u56e0\u6c89\u9ed8\u6548\u679c\uff0c\u57282017\u5e749\u6708\u4e09\u671f\u4e34\u5e8a\u8bd5\u9a8c\u4e5f\u53d6\u5f97\u4e86\u6781\u4f73\u7684\u6210\u679c\uff0c\u8fbe\u5230\u4e86\u4e3b\u8981\u7ec8\u70b9\u548c\u6240\u6709\u6b21\u8981\u7ec8\u70b9\u3002<\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\"><br  \/><\/span><\/p>\n<section  label=\"Powered by 135editor.com\" style=\"font-size:16px;font-family:\u5fae\u8f6f\u96c5\u9ed1;\">\n<section  label=\"Powered by 135editor.com\">\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;display: inline-block;box-sizing: border-box;\"  >\n<p style=\"margin-top: 8px;padding-right: 10px;font-weight: bold;line-height: 28px;max-width: 100%;color: rgb(0, 128, 255);min-height: 32px;border-bottom: 1.5px solid rgb(0, 128, 255);border-top-color: rgb(0, 128, 255);border-right-color: rgb(0, 128, 255);border-left-color: rgb(0, 128, 255);text-align: justify;\"><span class=\"autonum\"  placeholder=\"1\" style=\"color: rgb(255, 255, 255);display: block;float: left;line-height: 20px;margin-right: 8px;max-width: 100%;padding: 4px 10px;background-color: rgb(0, 128, 255);word-wrap: break-word !important;\" title=\"\">5<\/span><strong style=\"font-family: &quot;Helvetica Neue&quot;, Helvetica, &quot;Hiragino Sans GB&quot;, &quot;Microsoft YaHei&quot;, Arial, sans-serif;\">\u524d\u666f\u53ca\u5c55\u671b<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\"><\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">LNP-siRNA\u7cfb\u7edf\u5728\u809d\u7ec6\u80de\u4e2d\u6c89\u9ed8TTR\u57fa\u56e0\u7684\u6210\u529f\u6253\u5f00\u4e86\u4e00\u6247\u6c89\u9ed8\u809d\u7ec6\u80de\u4e2d\u7684\u57fa\u56e0\u7684\u5927\u95e8\uff0c\u800c\u51e0\u4e4e\u4efb\u4f55\u75be\u75c5\u90fd\u53ef\u4ee5\u901a\u8fc7\u6c89\u9ed8\u75c5\u7406\u6027\u57fa\u56e0\u3001\u8868\u8fbe\u6709\u76ca\u86cb\u767d\u6216\u7f16\u8f91\u6709\u7f3a\u9677\u7684\u57fa\u56e0\u6765\u6cbb\u7597\u3002\u4f46\u672a\u6765\u5176\u4ed6\u7684\u5e94\u7528\u4e0d\u4ec5\u4ec5\u5c40\u9650\u4e8e\u809d\u810f\u7ec6\u80de\uff0c\u4f7f\u7528LNP\u7cfb\u7edf\u53ef\u4ee5\u6c89\u9ed8\u809d\u810f\u4ee5\u5916\u7684\u7ec4\u7ec7\u4e2d\u7684\u57fa\u56e0\uff0c\u5982\u6c89\u9ed8\u81f4\u764c\u57fa\u56e0\uff0c\u6cbb\u7597\u8bf8\u5982\u764c\u75c7\u7684\u75be\u75c5\u3002\u53e6\u4e00\u65b9\u9762\uff0cLNP\u6280\u672f\u7684\u5ef6\u4f38\u5230\u9012\u9001mRNA\u4e3a\u6cbb\u7597\u6027\u86cb\u767d\u8d28\u7684\u8868\u8fbe\u63d0\u4f9b\u53ef\u80fd\uff0c\u53ef\u4ee5\u6cbb\u7597\u5404\u79cd\u9700\u8981\u86cb\u767d\u8d28\u66ff\u4ee3\u7684\u75be\u75c5\u3002\u6700\u540e\uff0c\u6269\u5c55CRSPR-CAS\u5728\u4f53\u5185\u8fdb\u884c\u57fa\u56e0\u7f16\u8f91\u7684\u53ef\u4ee5\u6cbb\u7597\u8bb8\u591a\u9057\u4f20\u75c5\u3002\u5176\u4e2d\u4e00\u4e9b\u76ee\u524d\u6b63\u5728\u4e34\u5e8a\u8bd5\u9a8c\u4e2d\u8fdb\u884c\u8bc4\u4f30<\/span><span style=\"font-size: 15px;color: rgb(0, 122, 170);\">(\u88682)<\/span><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">\u3002<\/span><\/p>\n<p style=\"text-align: justify;line-height: normal;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\"><br  \/><\/span><\/p>\n<p style=\"text-align: center;\"><img class=\"\"      style=\"\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/09\/5-1537927000.jpg\" alt=\"LNP\uff1a\u4fc3\u8fdbRNAi\u7531\u7406\u8bba\u5230\u4e34\u5e8a\u5e94\u7528\" title=\"LNP\uff1a\u4fc3\u8fdbRNAi\u7531\u7406\u8bba\u5230\u4e34\u5e8a\u5e94\u7528\"  \/><\/p>\n<p style=\"text-align: justify;line-height: normal;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\"><\/span><br  \/><\/p>\n<h2 style=\"line-height: 1.75em;text-align: justify;\"><span style=\"color: rgb(0, 128, 255);\"><strong><span style=\"color: rgb(0, 128, 255);font-size: 15px;\">5.1 \u809d\u5916\u9776\u533aLNP-siRNA\u7684\u8bbe\u8ba1<\/span><\/strong><\/span><\/h2>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">LNP siRNA\u7ed9\u836f\u7cfb\u7edf\u9776\u5411\u809d\u5916\u7ec4\u7ec7 LNP siRNA\u836f\u7269\u5728\u809d\u7ec6\u80de\u4e2d\u6709\u8bb8\u591a\u6f5c\u5728\u7684\u4e34\u5e8a\u5e94\u7528\u4ef7\u503c\uff0c\u7531ApoE\u4ecb\u5bfc\u7684\u8102\u86cb\u767d\u9897\u7c92\u7684\u6444\u53d6\u4e5f\u53ef\u53d1\u751f\u5728\u8111\u7b49\u5176\u5b83\u7ec4\u7ec7\u4e2d\u3002\u7531\u4e8e\u5b58\u5728\u8840\u8111\u5c4f\u969c\uff0c\u4f4e\u5bc6\u5ea6\u8102\u86cb\u767d(LDL)\u4e2d\u7684\u80c6\u56fa\u9187\u4e0d\u80fd\u5230\u8fbe\u5927\u8111\u3002\u56e0\u6b64\uff0c\u5927\u8111\u5408\u6210\u81ea\u5df1\u7684\u80c6\u56fa\u9187\uff0c\u5e76\u5229\u7528ApoE\u5c06\u80c6\u56fa\u9187\u8f6c\u8fd0\u5230\u795e\u7ecf\u5143\uff0c\u901a\u8fc7ApoE\u4ecb\u5bfc\u7684\u5185\u541e\u4f5c\u7528(\u7c7b\u4f3c\u4e8e\u809d\u7ec6\u80de)\u8fdb\u884c\u6444\u53d6\u3002\u5df2\u7ecf\u8bc1\u660e\uff0c\u5728\u809d\u7ec6\u80de\u4e2d\u5177\u6709\u57fa\u56e0\u6c89\u9ed8\u6709\u6548\u6027\u7684LNP siRNA\u7cfb\u7edf\uff0c\u7ecf\u5927\u8111\u76ae\u5c42\u5185\u7ed9\u836f\u540e\u5728\u795e\u7ecf\u5143\u4e2d\u5177\u6709\u76f8\u540c\u7684\u57fa\u56e0\u6c89\u9ed8\u6709\u6548\u6027\uff0c\u9700\u8981\u901a\u8fc7\u8111\u5ba4\u53ca\u8111\u9798\u5185\u5185\u7ed9\u836f\u5b9e\u73b0\u6ce8\u5c04\u90e8\u4f4d\u5468\u56f4\u7684\u6c89\u9ed8\u3002\u4e00\u9879\u5173\u4e8eLNP-siRNA\u4ecb\u5bfc\u7684\u57fa\u56e0\u6572\u9664\u5b9e\u9a8c\u4e2d\uff0c\u5b9e\u73b0\u4e86\u5bf9SLC26A11\u7684\u6291\u5236\uff0c\u663e\u8457\u51cf\u8f7b\u4e86\u795e\u7ecf\u5143\u80bf\u80c0\u3002\u8fd9\u4e9b\u7814\u7a76\u8bc1\u5b9eLNP-siRNA\u5177\u6709\u9776\u6807\u8bc6\u522b\u7684\u6f5c\u529b\uff0c\u4ee5\u53ca\u901a\u8fc7\u9798\u5185\u3001\u5ba4\u95f4\u6216\u9798\u5185\u9014\u5f84\u76f4\u63a5\u7ed9\u836f\u7684\u6cbb\u7597\u4e25\u91cd\u795e\u7ecf\u9000\u884c\u6027\u75be\u75c5\u7684\u5e94\u7528\u6f5c\u529b<\/span><\/p>\n<h2 style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">&nbsp;<\/span><\/h2>\n<h2 style=\"line-height: 1.75em;text-align: justify;\"><span style=\"color: rgb(0, 128, 255);\"><strong><span style=\"font-size: 15px;\">5.2 \u7528\u4e8e\u57fa\u56e0\u8868\u8fbe\u548c\u7f16\u8f91\u7684LNP\u8bbe\u8ba1<\/span><\/strong><\/span><\/h2>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">\u7814\u7a76\u8868\u660e\uff0c\u4e59\u9187\u8d1f\u8f7dLNP siRNA\u7cfb\u7edf\u5236\u5242\u5236\u5907\u6280\u672f\u53ca\u8bbe\u65bd\u540c\u6837\u9002\u7528\u4e8e\u5236\u5907\u751f\u4ea7LNP mRNA\u548cLNP\u8d28\u7c92(pDNA)\u7cfb\u7edf\u3002\u76ee\u524d\uff0c\u5df2\u7ecf\u5f00\u5c55\u4e86\u5927\u91cf\u4f18\u5316\u8f7d\u8fd0mRNA\u7684\u79bb\u5b50\u5316\u7684\u9633\u79bb\u5b50\u8102\u8d28\u7684\u7684\u7684\u7814\u53d1\uff0c\u5982\u9759\u8109\u6ce8\u5c04\u542b\u6709\u672a\u7ecf\u4fee\u9970\u7684\u3001\u7f16\u7801\u4fc3\u7ea2\u7ec6\u80de\u751f\u6210\u7d20(EPO)\u7684\u5e8f\u5217\u5de5\u7a0bmRNA-LNP\uff0c\u5728\u732a\u548c\u7334\u4e0a\u5b9e\u9a8c\u90fd\u53ef\u4ee5\u5347\u9ad8Epo\u6c34\u5e73\uff0c\u589e\u52a0\u7f51\u7ec7\u7ea2\u7ec6\u80de\u6570\uff0c\u5e76\u63d0\u9ad8\u7ea2\u7ec6\u80de\u538b\u79ef\u3002\u8fd9\u8bf4\u660e\u4e86\u5229\u7528\u809d\u810f\u4f5c\u4e3a\u751f\u7269\u53cd\u5e94\u5668\u8868\u8fbe\u6cbb\u7597\u86cb\u767d\u7684\u53ef\u80fd\u6027\u3002<\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><br  \/><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">\u4ee5\u8fd9\u79cd\u65b9\u5f0f\u4f7f\u809d\u810f\u4f5c\u4e3a\u751f\u7269\u53cd\u5e94\u5668\u4e3a\u591a\u79cd\u75be\u75c5\u6cbb\u7597\u5f00\u8f9f\u4e86\u65b0\u7684\u9014\u5f84\uff0cLNP-mRNA\u7cfb\u7edf\u5728\u201c\u4e3b\u52a8\u201d\u514d\u75ab\u65b9\u9762\u7684\u6f5c\u5728\u5e94\u7528\u3002\u76ae\u4e0b\u3001\u808c\u8089\u6216\u76ae\u5185\u6ce8\u5c04\u542b\u6709\u7f16\u7801\u591a\u79cd\u4f20\u67d3\u75c5\u76f8\u5173\u86cb\u767d\u6297\u539fmRNA\u7684 LNP\u7cfb\u7edf\uff0c\u5bfc\u81f4\u556e\u9f7f\u7c7b\u52a8\u7269\u548c\u975e\u4eba\u7075\u957f\u7c7b\u52a8\u7269\u5b8c\u5168\u514d\u53d7\u5be8\u5361\u75c5\u6bd2\u611f\u67d3\u7684\u75be\u75c5\u7684\u5f71\u54cd\uff0c\u5728\u4e34\u5e8a\u524d\u6a21\u578b\u4e2d\u4ea7\u751f\u4e86\u4ee4\u4eba\u5370\u8c61\u6df1\u523b\u7684\u514d\u75ab\u5e94\u7b54\u3002<\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><br  \/><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><strong><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">LNP-mRNA\u4e5f\u663e\u793a\u51fa\u4f5c\u4e3a\u75ab\u82d7\u7684\u6f5c\u5728\u5e94\u7528\u53ef\u80fd\u3002<\/span><\/strong><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">Kranz\u7b49\u4eba\u7814\u7a76\u8868\u660eLNP-mRNA\u5728\u6811\u7a81\u72b6\u7ec6\u80de\u514d\u75ab\u6cbb\u7597\u4e2d\uff0c\u53ef\u4ee5\u8bf1\u5bfcDC\u7ec6\u80de\u4e2d\u6297\u539f\u7684\u8868\u8fbe\u3002\u6b64\u5916\uff0c\u8fd8\u53ef\u4ee5\u5728\u9ed1\u8272\u7d20\u7624\u3001\u7ed3\u76f4\u80a0\u764c\u548c\u80ba\u764c\u6a21\u578b\u4e2d\u8bf1\u5bfc\u4e86\u5f3a\u70c8\u7684\u6297\u80bf\u7624\u53cd\u5e94\uff0c\u8fd8\u5177\u6709\u5f88\u9ad8\u7684\u5b89\u5168\u6027\u548c\u8010\u53d7\u6027\u3002Yin\u7b49\u4eba\u5229\u7528LNP-CRISPR\u6280\u672f\u9776\u5411\u5c0f\u9f20\u7684PCSK9\u57fa\u56e0\u6cbb\u7597\u9ad8\u80c6\u56fa\u9187\u8840\u75c7\uff0c\u5728\u4e00\u6b21\u6ce8\u5c04\u540e\u53ef\u4ee5\u5c06\u5c0f\u9f20\u7684\u80c6\u56fa\u9187\u964d\u4f4e30~40%\u3002<\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\"><br  \/><\/span><\/p>\n<h2 style=\"line-height: 1.75em;text-align: justify;\"><span style=\"color: rgb(0, 128, 255);\"><strong><span style=\"font-size: 15px;\">5.3 \u672a\u6765<\/span><\/strong><\/span><\/h2>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">\u7ee7\u7b2c\u4e00\u4ee3\u5c0f\u5206\u5b50\u836f\u7269\u3001\u7b2c\u4e8c\u4ee3\u86cb\u767d\u7c7b\u751f\u7269\u836f\u4e4b\u540e\uff0c<strong>\u7b2c\u4e09\u4ee3\u201c\u667a\u80fd\u201d\u7eb3\u7c73\u836f\u7269\u5c06\u6700\u5927\u9650\u5ea6\u5730\u5229\u7528\u751f\u7269\u4f53\u5185\u8fc7\u7a0b\uff0c\u5b9e\u73b0\u4f53\u5185\u5e94\u7528\u7684\u9ad8\u5ea6\u9776\u5411\u6027\uff0c\u964d\u4f4e\u5bf9\u4eba\u4f53\u7684\u6bd2\u526f\u4f5c\u7528\u3002<\/strong>\u663e\u7136\uff0c\u542b\u6709\u6838\u9178\u836f\u7269\u7684LNP\u7cfb\u7edf\u5c06\u5728\u8fd9\u4e00\u8fdb\u5316\u4e2d\u626e\u6f14\u91cd\u8981\u89d2\u8272\u3002\u4e00\u4e2a\u663e\u8457\u7684\u65b0\u7279\u5f81\u662f\u5f00\u53d1\u4e86\u80fd\u591f \u201c\u5305\u88f9\u201d\u5e26\u8d1f\u7535\u8377\u7684\u5927\u5206\u5b50siRNA\u7684LNP\u7ed9\u836f\u7cfb\u7edf\u3002LNP\u7ed9\u836f\u7cfb\u7edf\u5728\u4f53\u5185\u80fd\u9012\u9001\u66f4\u5927\u5206\u5b50\u91cf\u7684mRNA \u53ca\u8d28\u7c92DNA\u53d1\u6325\u751f\u7406\u529f\u80fd\uff0c\u53ef\u80fd\u5141\u8bb8\u6cbb\u7597\u8bb8\u591a\u57fa\u56e0\u7a81\u53d8\u5f15\u8d77\u75be\u75c5\u6240\u9700\u7684\u7cbe\u786e\u57fa\u56e0\u7597\u6cd5\u4ed8\u8bf8\u5b9e\u65bd\u3002\u6b64\u5916\uff0c\u9664\u4e86\u6709\u6548\u8d1f\u8f7d\u6838\u9178\u836f\u7269\u5916\uff0cLNP\u7cfb\u7edf\u53ef\u5305\u542b\u591a\u79cd\u53ef\u4fee\u9970\u7684\u7ec4\u5206\uff0c\u7c7b\u4f3c\u63d0\u4f9b\u4e86\u4e00\u4e2a\u76f8\u5f53\u5927\u7684\u5de5\u5177\u7bb1\uff0c\u53ef\u6700\u5927\u7a0b\u5ea6\u5730\u53d1\u6325\u8d1f\u8f7d\u836f\u7269\u7684\u6548\u529b\u53ca\u964d\u4f4e\u836f\u7269\u7684\u6bd2\u526f\u4f5c\u7528\u3002<\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">&nbsp;<\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">\u503c\u5f97\u6ce8\u610f\u7684\u662f\uff0c\u901a\u8fc7\u76ee\u524d\u7684LNP\u7cfb\u7edf\u66f4\u591a\u7684\u4f9d\u8d56\u4e8e\u201c\u81ea\u7136\u201d\u9776\u5411\u8fc7\u7a0b\uff0c\u5982\u9776\u5411\u809d\u7ec6\u80de\u7684ApoE\u4f9d\u8d56\u7684\u5185\u541e\u9014\u5f84\u3001\u6216\u7eb3\u7c73\u7c92\u5b50\u5bf9\u514d\u75ab\u7ec6\u80de\u7684\u5929\u7136\u9776\u5411\uff0c\u800c\u4e0d\u662f\u5229\u7528\u914d\u4f53\u9776\u5411\u3002\u6b64\u5916\uff0cLNP RNA\uff0c\u6216DNA\u5236\u5242\u4f53\u5185\u5e94\u7528\u65f6\u673a\u4f53\uff0c\u5176\u4e2d\u805a\u5408\u7269\u4ea7\u751f\u7684\u514d\u75ab\u5e94\u7b54\u53cd\u5e94\uff1b\u4ee5\u53ca\u5728\u809d\u810f\u5916\u7684\u5176\u5b83\u7ec4\u7ec7\u4e2d\u5982\u4f55\u53d1\u6325siRNA\u3001mRNA\u3001\u53caDNA\u7684\u4f5c\u7528\u7684\u95ee\u9898\u90fd\u503c\u5f97\u5173\u6ce8\u3002<\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">&nbsp;<\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">\u91c7\u7528LNP\u7ed9\u836f\u7cfb\u7edf\u4f7f\u5f97Onpattro\u6210\u529f\u4e0a\u5e02\uff0c\u5fc5\u5c06\u5728\u5168\u4e16\u754c\u8303\u56f4\u5185\u63a8\u52a8 siRNA\u836f\u7269\u7ed9\u836f\u7cfb\u7edf\u53casiRNA\u836f\u7269\u5f00\u53d1\u7684\u7814\u7a76\u70ed\u6f6e\u3002&nbsp;<\/span><\/p>\n<p style=\"line-height: 1.75em;text-align: justify;\"><span style=\"font-size: 15px;color: rgb(0, 0, 0);\">&nbsp;<\/span><\/p>\n<p style=\"text-align: left;line-height: normal;\"><span style=\"font-size: 10px;color: rgb(136, 136, 136);\">\u53c2\u8003\u6587\u732e\uff1a<\/span><\/p>\n<p style=\"text-align: left;line-height: normal;\"><span style=\"font-size: 10px;color: rgb(136, 136, 136);\">[1] Kulkarni J A, Cullis P R, van der Meel R.Lipid Nanoparticles Enabling Gene Therapies: From Concepts to ClinicalUtility[J]. Nucleic Acid Therapeutics,2018,28(3):146-157.<\/span><\/p>\n<p style=\"text-align: left;line-height: normal;\"><span style=\"font-size: 10px;color: rgb(136, 136, 136);\">[2]\u5b63\u7231\u6c11\uff0c\u97e9\u519b\u9996\u6b3esiRNA\u836f\u7269Onpattro\u7684\u5236\u5242\u5b66\u6982\u8ff0<\/span><\/p>\n<p style=\"text-align: left;line-height: normal;\"><span style=\"font-size: 10px;color: rgb(136, 136, 136);\">[3]\u6731\u5174\u5168, \u9648\u5b81, \u5468\u9e4f. RNAi-\u751f\u547d\u79d1\u5b66\u7684\u53c8\u4e00\u91cd\u5927\u53d1\u73b0:2006\u5e74\u8bfa\u8d1d\u5c14\u751f\u7406\u5b66\/\u533b\u5b66\u5956\u4ecb\u7ecd[J].\u70ed\u5e26\u533b\u5b66\u6742\u5fd7, 2007, 7(1):1-2.<\/span><\/p>\n<p style=\"text-align: left;line-height: normal;\"><span style=\"font-size: 10px;color: rgb(136, 136, 136);\"><br  \/><\/span><\/p>\n<section  label=\"Powered by 135editor.com\" style=\"font-size:16px;font-family:\u5fae\u8f6f\u96c5\u9ed1;\">\n<section  label=\"Powered by 135editor.com\">\n<section class=\"_135editor\"   style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section style=\"text-align: center;\"><img class=\"\"      style=\"width: 80%;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/09\/5-1537927000.gif\" alt=\"LNP\uff1a\u4fc3\u8fdbRNAi\u7531\u7406\u8bba\u5230\u4e34\u5e8a\u5e94\u7528\" title=\"LNP\uff1a\u4fc3\u8fdbRNAi\u7531\u7406\u8bba\u5230\u4e34\u5e8a\u5e94\u7528\"  \/><\/section>\n<\/section>\n<p style=\"text-align: center;line-height: 1.5em;\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzA5MjEzODQ3NA==&amp;mid=2655762905&amp;idx=1&amp;sn=38445b75639f99d39edb697856ac5cde&amp;chksm=8bcea75cbcb92e4a8b68b77faca3b71be87b28f55838b7a06ec81bfc36e2024abcb0e860a05a&amp;scene=21#wechat_redirect\" target=\"_blank\" style=\"text-decoration: underline;font-size: 14px;\"><span style=\"font-size: 14px;\">2018\u5e748\u6708\u5168\u7403\u6279\u51c6\u65b0\u836f\u6982\u51b5<\/span><\/a><br  \/><\/p>\n<p style=\"text-align: center;line-height: 1.5em;\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzA5MjEzODQ3NA==&amp;mid=2655762730&amp;idx=1&amp;sn=1e7cd8a6771f1081181c7f9f5bd48b8a&amp;chksm=8bcea7afbcb92eb9e0cd65a89eb9e9452d175f0e767ae0ffb2e055ba496f8976e353371cf6e3&amp;scene=21#wechat_redirect\" target=\"_blank\" style=\"text-decoration: underline;font-size: 14px;\"><span style=\"font-size: 14px;\">2018\u5e747\u6708\u5168\u7403\u6279\u51c6\u65b0\u836f\u6982\u51b5<\/span><\/a><br  \/><\/p>\n<p style=\"text-align: center;line-height: 1.5em;\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzA5MjEzODQ3NA==&amp;mid=2655762864&amp;idx=1&amp;sn=208398104406907496d9596d3de57d96&amp;chksm=8bcea735bcb92e230d304e63af247fd22a5fa8f3e765d0684b80c84744d1e4189959ffd88baa&amp;scene=21#wechat_redirect\" target=\"_blank\" style=\"text-decoration: underline;font-size: 14px;\"><span style=\"font-size: 14px;\">\u67e5\u836f\u4f01\u3001\u627e\u533b\u836f\u56ed\u533a\uff1f\u4e0a\u836f\u6e21App<\/span><\/a><br  \/><\/p>\n<p style=\"text-align: center;line-height: 1.5em;\"><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzA5MjEzODQ3NA==&amp;mid=2655762860&amp;idx=2&amp;sn=aca75a88721aa548dede491c2d62f465&amp;chksm=8bcea729bcb92e3fc7a11d6a14428d303c9453061366e9d833f9fff9fb5b6d76972ce3eb4089&amp;scene=21#wechat_redirect\" target=\"_blank\" style=\"text-decoration: underline;font-size: 14px;\"><span style=\"font-size: 14px;\">\u836f\u6e21APP\u5168\u9762\u5347\u7ea7\u5566\uff01\u4e00\u89c8\u5168\u7403\u83b7\u6279\u65b0\u836f\uff0c\u4e2d\u56fd\u4e34\u5e8a\u9879\u76ee\uff0c\u884c\u4e1a\u878d\u8d44\u4fe1\u606f\uff0c\u5b9e\u65f6\u66f4\u65b0\uff0c\u5168\u8986\u76d6\uff01<\/span><\/a><br  \/><\/p>\n<\/section>\n<\/section>\n<p><br  \/><\/p>\n<p><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzA5MjEzODQ3NA==&amp;mid=2655762488&amp;idx=2&amp;sn=8f278096debef35b578d6e03e56e3488&amp;chksm=8bcea6bdbcb92fabd503d5bb73cc273697ebf0769fbb7bfb96056b5dce3644647bd18e9d2fbe&amp;scene=21#wechat_redirect\" target=\"_blank\" style=\"text-align: center;white-space: normal;\"><span class=\"js_jump_icon h5_image_link\"  style=\"top: auto;left: auto;right: auto;bottom: auto;\"><img class=\"\"               style=\"width: 100%;height: auto;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/09\/6-1537927000.jpg\" alt=\"LNP\uff1a\u4fc3\u8fdbRNAi\u7531\u7406\u8bba\u5230\u4e34\u5e8a\u5e94\u7528\" title=\"LNP\uff1a\u4fc3\u8fdbRNAi\u7531\u7406\u8bba\u5230\u4e34\u5e8a\u5e94\u7528\"  \/><\/span><\/a><\/p>\n<p style=\"text-align: center;\"><a href=\"https:\/\/mp.weixin.qq.com\/s?__biz=MzA5MjEzODQ3NA==&amp;mid=2655756292&amp;idx=6&amp;sn=8ae873807e0f13e4eaaea32a11123559&amp;chksm=8bcebe81bcb9379718fa3f7c97699a16524969f4b74d9a3e1b46329a304786628bc806562b51&amp;scene=21#wechat_redirect\" target=\"_blank\"><span class=\"js_jump_icon h5_image_link\"  style=\"top: auto;left: auto;margin: 0px;right: auto;bottom: auto;\"><img class=\"\"               style=\"margin: 0px;width: 100%;height: auto;top: auto;left: auto;right: auto;bottom: auto;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/09\/8-1537927000.jpeg\" alt=\"LNP\uff1a\u4fc3\u8fdbRNAi\u7531\u7406\u8bba\u5230\u4e34\u5e8a\u5e94\u7528\" title=\"LNP\uff1a\u4fc3\u8fdbRNAi\u7531\u7406\u8bba\u5230\u4e34\u5e8a\u5e94\u7528\"  \/><\/span><\/a><\/p>\n<p style=\"text-align: center;\"><img class=\"\"           style=\"width: 100%;height: auto;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/09\/5-1537927001.jpeg\" alt=\"LNP\uff1a\u4fc3\u8fdbRNAi\u7531\u7406\u8bba\u5230\u4e34\u5e8a\u5e94\u7528\" title=\"LNP\uff1a\u4fc3\u8fdbRNAi\u7531\u7406\u8bba\u5230\u4e34\u5e8a\u5e94\u7528\"  \/><\/p>\n<p style=\"text-align: center;\"><img class=\"\"           style=\"width: 100%;height: auto;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/09\/9-1537927001.jpeg\" alt=\"LNP\uff1a\u4fc3\u8fdbRNAi\u7531\u7406\u8bba\u5230\u4e34\u5e8a\u5e94\u7528\" title=\"LNP\uff1a\u4fc3\u8fdbRNAi\u7531\u7406\u8bba\u5230\u4e34\u5e8a\u5e94\u7528\"  \/><\/p>\n<section class=\"RankEditor\" style=\"margin-top: 20px;\">\n<section style=\"display: inline-block;vertical-align: middle;width:12%;box-sizing: border-box;\">\n<section style=\"margin-top: 0.5em;margin-bottom: 0.5em;box-sizing: border-box;\"><\/section>\n<\/section>\n<section style=\"display:inline-block;width: 88%;padding:5px;box-sizing: border-box;vertical-align:middle;\">\n<p class=\"active brush\" style=\"box-sizing: border-box;font-size: 16px;color: rgb(121,121,121);min-width:1px;\"><span style=\"font-size: 15px;\">\u7acb\u5373\u89e3\u9501\u4f60\u7684\u638c\u4e0a\u4e13\u4e1a\u5de5\u5177\uff01<\/span><\/p>\n<\/section>\n<\/section><\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u5bfc\u8bfb\uff1a8\u670811\u65e5\uff0c\u7f8e\u56fdFDA\u5ba3\u5e03\u9996\u6b3e\u5c0f\u5e72\u6270RNA (siRNA)\u836f\u7269Onpattro (patisiran)\u4e0a 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