{"id":49744,"date":"2018-09-25T01:00:00","date_gmt":"2018-09-26T02:01:17","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/2018\/09\/26\/%e6%b5%8b%e5%ba%8f%e5%85%ac%e5%8f%b8%e6%af%94%e8%be%83%e4%ba%8c%e4%bb%a3%e6%b5%8b%e5%ba%8f%e7%bb%93%e6%9e%9c%ef%bc%9a-hiseq-x-ten-vs-novaseq-6000\/"},"modified":"2018-09-26T10:01:18","modified_gmt":"2018-09-26T02:01:18","slug":"%e6%b5%8b%e5%ba%8f%e5%85%ac%e5%8f%b8%e6%af%94%e8%be%83%e4%ba%8c%e4%bb%a3%e6%b5%8b%e5%ba%8f%e7%bb%93%e6%9e%9c%ef%bc%9a-hiseq-x-ten-vs-novaseq-6000","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/49744\/","title":{"rendered":"\u6d4b\u5e8f\u516c\u53f8\u6bd4\u8f83\u4e8c\u4ee3\u6d4b\u5e8f\u7ed3\u679c\uff1a Hiseq X ten VS NovaSeq 6000"},"content":{"rendered":"
\n

\"\u6d4b\u5e8f\u516c\u53f8\u6bd4\u8f83\u4e8c\u4ee3\u6d4b\u5e8f\u7ed3\u679c\uff1a<\/p>\n

\u4ece1977\u5e74Sanger\u53d1\u660e\u4e86\u53cc\u8131\u6c27\u94fe\u7ec8\u6b62\u6cd5\u4e00\u4ee3\u6d4b\u5e8f\u6280\u672f\u5f00\u59cb\uff0c\u6d4b\u5e8f\u6280\u672f\u53d1\u5c55\u81f3\u4eca\u5df2\u6709\u56db\u5341\u591a\u5e74\u65f6\u95f4\uff0c\u5148\u540e\u7ecf\u5386\u4e86\u4ee5GS FLX\u3001Solexa\u3001SOLID\u4e3a\u57fa\u7840\u7684\u4e8c\u4ee3\u6d4b\u5e8f\u6280\u672f\uff0c\u4ee5\u53ca\u57fa\u4e8e\u5355\u5206\u5b50\u5b9e\u65f6\u6d4b\u5e8f\uff08SMRT\uff09\u548c\u7eb3\u7c73\u5b54\u6d4b\u5e8f\u6280\u672f\u7684\u4e09\u4ee3\u6d4b\u5e8f\u6280\u672f\u3002\u867d\u7136\u4e09\u4ee3\u6d4b\u5e8f\u5728\u84ec\u52c3\u53d1\u5c55\uff0c\u5e76\u5728\u57fa\u56e0\u7ec4\u548c\u8f6c\u5f55\u7ec4\u6d4b\u5e8f\u7b49\u9886\u57df\u5c55\u73b0\u51fa\u524d\u6240\u672a\u6709\u7684\u4f18\u52bf\uff0c\u4f46\u9650\u4e8e\u6210\u672c\u95ee\u9898\uff0c\u5176\u5e94\u7528\u8303\u56f4\u5c1a\u4e0d\u53ca\u4e8c\u4ee3\u6d4b\u5e8f\u3002
<\/p>\n


<\/p>\n

\u3000\u3000\u4e8c\u4ee3\u6d4b\u5e8f\u6280\u672f\u4ee5\u5176\u77ed\u8bfb\u957f\u3001\u9ad8\u901a\u91cf\u3001\u51c6\u786e\u6027\u9ad8\u7684\u7279\u70b9\uff0c\u4ecd\u5728\u6d4b\u5e8f\u5e02\u573a\u4e0a\u5360\u4f18\u52bf\u5730\u4f4d\u3002\u4ee5Illumina Solexa\u4e3a\u4f8b\uff0c\u9996\u5148\u5229\u7528\u8d85\u58f0\u6ce2\u5c06DNA\u6253\u65ad\u6210200-500bp\u5c0f\u7247\u6bb5\u6587\u5e93\uff0c\u52a0\u63a5\u5934\u540eDNA\u7247\u6bb5\u968f\u673a\u9644\u7740\u4e8eflowcell\u8868\u9762\uff0c\u7ecf\u8fc7\u6865\u5f0fPCR\u6269\u589e\u5f62\u6210\u201cDNA\u7c07\u201d\uff0c\u5b9e\u73b0\u78b1\u57fa\u4fe1\u53f7\u5f3a\u5ea6\u653e\u5927\uff0c\u91c7\u7528\u8fb9\u5408\u6210\u8fb9\u6d4b\u5e8f\u7684\u65b9\u6cd5\uff0c\u8fdb\u884c\u5168\u57fa\u56e0\u7ec4\u5168\u9762\uff0c\u51c6\u786e\u7684\u6d4b\u5e8f\u3002<\/p>\n

\"\u6d4b\u5e8f\u516c\u53f8\u6bd4\u8f83\u4e8c\u4ee3\u6d4b\u5e8f\u7ed3\u679c\uff1a<\/p>\n

\u56fe1 Hiseq Xten (\u5de6) \u4e0e NovaSeq 6000\uff08\u53f3\uff09<\/strong><\/p>\n


<\/p>\n

\u3000\u30002014\u5e74Illumina\u63a8\u51faHiSeq X Ten\u6d4b\u5e8f\u4eea\uff0c\u5b83\u5229\u7528\u6570\u5341\u4ebf\u4e2a\u7eb3\u7c73\u5b54\u7684\u6d41\u52a8\u69fd\uff0c\u8f83\u5927\u7f29\u77ed\u4e86\u6d4b\u5e8f\u5468\u671f\u30022017\u5e74\u5b83\u53c8\u63a8\u51fa\u4e86\u65b0\u4e00\u4ee3\u6d4b\u5e8f\u4eeaNovaSeq\u7cfb\u5217\uff0c\u6211\u4eec\u4ee5\u76f8\u540c\u6587\u5e93\u5206\u522b\u8fdb\u884cHiseq Xten\u7cfb\u5217\u548cNovaSeq\u7cfb\u5217\u6d4b\u5e8f\uff0cDNA\u91cd\u6d4b\u5e8f\u4ea7\u51fa\u6570\u636e\u6307\u6807\u5982\u4e0b\uff1a<\/p>\n


<\/p>\n

\u88681 DNA\u91cd\u6d4b\u5e8f\u7ed3\u679c\u6bd4\u8f83<\/strong><\/p>\n

\"\u6d4b\u5e8f\u516c\u53f8\u6bd4\u8f83\u4e8c\u4ee3\u6d4b\u5e8f\u7ed3\u679c\uff1a<\/p>\n

\u770b\u5b8c\u91cd\u6d4b\u5e8f\uff0c\u518d\u770b\u770b\u8f6c\u5f55\u7ec4\u6587\u5e93\u6d4b\u5e8f\u6bd4\u8f83\uff1a<\/p>\n


<\/p>\n

\u88682 \u8f6c\u5f55\u7ec4\u6d4b\u5e8f\u7ed3\u679c\u6bd4\u8f83<\/strong><\/p>\n

\n

\u57fa\u4e8e\u4ee5\u4e0a\u7ed3\u679c\uff0c\u56fe\u8c31\u541b\u603b\u7ed3\u4e86\u4ee5\u4e0b\u51e0\u70b9\uff1a<\/p>\n

1.\u6d4b\u5e8f\u539f\u7406<\/strong>\uff1aX-ten\u4e0eNova6000\u6d4b\u5e8f\u539f\u7406\u5747\u662f\u57fa\u4e8esolexa\u7684\u8fb9\u5408\u6210\u8fb9\u6d4b\u5e8f\u7684\u539f\u7406\uff1bNova6000\u91c7\u7528Illumina\u7684EX-AMP\u7c07\u751f\u6210\u6280\u672f\uff0c\u4ee5\u53ca\u65b0\u4e00\u4ee3\u7684Patterned Flow Cell\u3002<\/p>\n

2.Q30\u8d28\u91cf\u503c<\/strong>\uff1a\u5728\u5b9e\u9645\u6d4b\u5e8f\u4e2dNova6000\u7684Q30\u76f8\u5bf9\u4e8eX-ten\u66f4\u7a33\u5b9a\u4e14\u6d4b\u5e8f\u65f6\u957f\u66f4\u77ed\uff0c\u8bd5\u5242\u8870\u51cf\u5bf9\u8d28\u91cf\u5f71\u54cd\u66f4\u5c0f\uff0c\u6574\u4f53\u7684Q30 Nova6000\u8981\u4f18\u4e8eX-ten\u3002<\/p>\n

3.\u6d4b\u5e8f\u65b9\u5f0f<\/strong>\uff1a\u53d7\u9650\u4e8eX-ten\u7684\u63a7\u5236\u8f6f\u4ef6\u4ee5\u53ca\u8bd5\u5242\u7b49\u56e0\u7d20\uff0cX-ten\u53ea\u80fd\u8fdb\u884c\u5355Index\u7684\u6d4b\u5e8f\u8bc6\u522b\uff1b\u800cNova6000\u53ef\u4ee5\u8fdb\u884cI7 I5\u53cc\u7aefIndex\u7684\u6d4b\u5e8f\uff0c\u7406\u8bba\u4e0a\u53ef\u4ee5\u505a\u5230\u66f4\u7cbe\u51c6\u7684\u8bc6\u522b\u3002<\/p>\n

4.DNA\u6587\u5e93\u5197\u4f59\u5ea6<\/strong>\uff1aNova6000\u660e\u786e\u4f18\u4e8eX-ten\u5e73\u53f0\u3002<\/p>\n

\u3000\u3000\u6709\u6ca1\u6709\u53d1\u73b0\u968f\u7740\u4e8c\u4ee3\u6d4b\u5e8f\u4eea\u5668\u7684\u53d1\u5c55\uff0c\u6d4b\u5e8f\u7ed3\u679c\u771f\u662f\u53c8\u5feb\u53c8\u597d\uff0c\u76ee\u524d\u4e8c\u4ee3\u6d4b\u5e8f\u8f83\u591a\u7684\u5e94\u7528\u4e8e\u57fa\u56e0\u7ec4\u91cd\u6d4b\u5e8f\uff0c\u8f6c\u5f55\u7ec4\u5206\u6790\uff0c\u5c0f\u5206\u5b50RNA\u7814\u7a76\u7b49\u9886\u57df\u3002\u57fa\u4e8e\u4e8c\u4ee3\u6d4b\u5e8f\u6280\u672f\u8fdb\u884c\u9057\u4f20\u56fe\u8c31\u6784\u5efa\uff0c\u57fa\u56e0\u5b9a\u4f4d\u7684\u7814\u7a76\u4e5f\u8d8a\u6765\u8d8a\u591a\u3002<\/p>\n


<\/p>\n<\/p><\/div>\n","protected":false},"excerpt":{"rendered":"

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