{"id":54896,"date":"2018-09-26T01:00:00","date_gmt":"2018-09-30T01:59:35","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/2018\/09\/30\/%e3%80%90%e5%88%86%e6%9e%90%e3%80%91%e5%ae%9a%e9%87%8f%e5%8c%96%e5%ad%a6%e8%9b%8b%e7%99%bd%e8%b4%a8%e7%bb%84%e5%ad%a6%e6%96%b0%e7%ad%96%e7%95%a5\/"},"modified":"2018-09-30T09:59:37","modified_gmt":"2018-09-30T01:59:37","slug":"%e3%80%90%e5%88%86%e6%9e%90%e3%80%91%e5%ae%9a%e9%87%8f%e5%8c%96%e5%ad%a6%e8%9b%8b%e7%99%bd%e8%b4%a8%e7%bb%84%e5%ad%a6%e6%96%b0%e7%ad%96%e7%95%a5","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/54896\/","title":{"rendered":"\u3010\u5206\u6790\u3011\u5b9a\u91cf\u5316\u5b66\u86cb\u767d\u8d28\u7ec4\u5b66\u65b0\u7b56\u7565"},"content":{"rendered":"
\n

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<\/p>\n

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<\/span><\/p>\n

\u8fd1\u65e5\uff0c\u5317\u4eac\u5927\u5b66<\/strong>\u5316\u5b66\u5206\u5b50\u5de5\u7a0b\u5b66\u9662\u7684\u738b\u521d<\/strong>\u7814\u7a76\u5458\u56e2\u961f\u9996\u6b21\u5c06\u57fa\u4e8e\u540c\u4f4d\u7d20\u7684\u4e8c\u7532\u57fa\u5316\u6807\u8bb0\u6cd5\u4e0eTOP-ABPP\u6280\u672f\u76f8\u7ed3\u5408<\/strong>\uff0c\u89c4\u907f\u6602\u8d35\u7684\u6c28\u57fa\u9178\u8bd5\u5242\u4ee5\u53ca\u590d\u6742\u7684\u5316\u5b66\u5408\u6210\uff0c\u53d1\u5c55\u4e86\u4e00\u79cd\u5bb9\u6613\u83b7\u5f97\u3001\u7ecf\u6d4e\uff0c\u5e76\u4e14\u4e0e\u591a\u79cd\u5546\u4e1a\u5316\u53ef\u5207\u5272\u6807\u7b7e\u517c\u5bb9\uff0c\u540c\u65f6\u53ef\u4ee5\u5b9e\u73b0\u4e09\u91cd\u6807\u8bb0\u5b9a\u91cf\u5316\u5b66\u86cb\u767d\u8d28\u7684\u65b9\u6cd5rdTOP-ABPP<\/strong>\u3002<\/span><\/p>\n

\"\u3010\u5206\u6790\u3011\u5b9a\u91cf\u5316\u5b66\u86cb\u767d\u8d28\u7ec4\u5b66\u65b0\u7b56\u7565\"<\/p>\n


<\/p>\n

\u5728\u8be5\u65b9\u6cd5\u4e2d\uff0c\u7ecf\u8fc7\u63a2\u9488\u5171\u4ef7\u6807\u8bb0\u3001\u751f\u7269\u6b63\u4ea4\u53cd\u5e94\u3001\u751f\u7269\u7d20\u5bcc\u96c6\u4ee5\u53ca\u4e32\u8054\u5207\u5272\u540e\uff0c\u542b\u6709\u63a2\u9488\u6807\u8bb0\u7684\u80bd\u6bb5\u88ab\u8f7b\u3001\u4e2d\u3001\u91cd\u4e09\u79cd\u540c\u4f4d\u7d20\u6807\u8bb0\u7684\u7532\u919b\u8bd5\u5242\u6807\u8bb0\u5e76\u88ab\u6c30\u57fa\u787c\u6c22\u5316\u94a0\u8fd8\u539f\uff0c\u5b9e\u73b0\u4e86\u5bf9\u80bd\u6bb5\u4e2dN\u7aef\u548c\u8d56\u6c28\u9178\u4fa7\u94fe\u6b8b\u57fa\u4e2d\u6c28\u57fa\u57fa\u56e2\u7684\u540c\u4f4d\u7d20\u4e8c\u7532\u57fa\u5316\u6807\u8bb0\uff0c\u5728\u901a\u8fc7\u9ad8\u5206\u8fa8\u6db2\u8d28\u8054\u7528\u4eea\u5bf9\u6837\u54c1\u5206\u6790\u540e\uff0c\u53ef\u4ee5\u901a\u8fc7\u5b9e\u9a8c\u5ba4\u5f00\u53d1\u7684CIMAGE\u8f6f\u4ef6\u8fdb\u884c\u4e09\u91cd\u5b9a\u91cf\u6570\u636e\u7684\u5206\u6790\u3002\u5b8c\u6210\u4e86\u5bf9rdTOP-ABPP\u65b9\u6cd5\u7684\u5efa\u7acb\u548c\u4f18\u5316\u540e\uff0c\u4f5c\u8005\u9996\u5148\u5c06\u8be5\u65b9\u6cd5\u4e0eisoTOP-ABPP\u5728\u5b9e\u9645\u6848\u4f8b\u8fdb\u884c\u4e86\u76f4\u63a5\u7684\u6bd4\u8f83\u3002Wang\u7b49\u4eba\u57282014\u5e74\u5229\u7528competitive isoTOP-ABPP\u7b56\u7565\u5b9a\u91cf\u5206\u6790\u4e86\u5168\u7ec6\u80de\u4e2d\u8102\u6e90\u6027\u4eb2\u7535\u5c0f\u5206\u5b50HNE\u7684\u4fee\u9970\u534a\u80f1\u6c28\u9178\u4f4d\u70b9\uff0c\u4f46\u4e3a\u4e86\u6bd4\u8f83\u4e0d\u540c\u6d53\u5ea6\u4e0b\u4fee\u9970\u6c34\u5e73\u7684\u53d8\u5316\uff0c\u4f5c\u8005\u9700\u8981\u5c06\u53ea\u6709\u4e8c\u91cd\u5b9a\u91cf\u80fd\u529b\u7684isoTOP-ABPP\u5b9e\u9a8c\u8fdb\u884c\u4e24\u6b21\u5e73\u884c\u7684\u64cd\u4f5c\uff0c\u6700\u540e\u53d6\u4e8c\u8005\u7684\u4ea4\u96c6\uff0c\u8d39\u65f6\u8d39\u529b\u800c\u4e14\u9020\u6210\u6570\u636e\u7684\u4e22\u5931\u3002\u800c\u5b8c\u6210\u540c\u6837\u7684\u5206\u6790\uff0c\u4f5c\u8005\u53ea\u9700\u8981\u64cd\u4f5crdTOP-ABPP\u4e00\u6b21\uff0c\u5c31\u53ef\u4ee5\u5b9e\u73b0DMSO\u300110 \u03bcM\u300150 \u03bcM\u4e09\u79cd\u4e0d\u540c\u5904\u7406\u6761\u4ef6\u4e0b\u4fee\u9970\u4f4d\u70b9\u7684\u51c6\u786e\u5b9a\u91cf\u3002\u6570\u636e\u5206\u6790\u8868\u660e\uff0crdTOP-ABPP\u7684\u5b9a\u91cf\u7ed3\u679c\u4e0d\u4ec5\u4e0ecompetitive isoTOP-ABPP\u5177\u6709\u8f83\u597d\u7684\u91cd\u53e0\uff08\u4f8b\u5982RTN4\u4e2d\u7684\u7b2c1111\u4f4d\uff0cEEF2\u4e2d\u7b2c41\u4f4d\uff0cFN3KRP\u4e2d\u7b2c24\u4f4d\u7b49HNE\u9ad8\u6d3b\u6027\u7684\u534a\u80f1\u6c28\u9178\u4f4d\u70b9\u90fd\u80fd\u901a\u8fc7\u4e24\u79cd\u65b9\u6cd5\u9274\u5b9a\uff09\uff0c\u800c\u4e14rdTOP-ABPP\u53ef\u9274\u5b9a\u8bb8\u591a\u989d\u5916\u7684\u5168\u65b0\u4f4d\u70b9\uff08HMOX2\u7684\u7b2c282\u4f4d\u534a\u80f1\u6c28\u9178\u4e0eVDAC2\u7684\u7b2c210\u4f4d\u534a\u80f1\u6c28\u9178\uff09\u3002\u8fd9\u4e00\u7ed3\u679c\u8bf4\u660erdTOP-ABPP\u5177\u6709\u4e0eisoTOP-ABPP\u7c7b\u4f3c\u751a\u81f3\u66f4\u597d\u7684\u5206\u6790\u80fd\u529b\uff0c\u540c\u65f6\u907f\u514d\u4e86isoTOP-ABPP\u5bf9\u4ef7\u683c\u6602\u8d35\u3001\u5408\u6210\u5197\u4f59\u7684\u540c\u4f4d\u7d20\u6807\u8bb0\u751f\u7269\u7d20\u63a2\u9488\u7684\u4f9d\u8d56\u3002<\/span><\/p>\n


<\/span><\/p>\n

rdTOP-ABPP\u9664\u4e86\u53ef\u4ee5\u5b9e\u73b0\u5bf9\u6d53\u5ea6\u68af\u5ea6\u7684\u5316\u5408\u7269\u4fee\u9970\u8fdb\u884c\u9274\u5b9a\u4e4b\u5916\uff0c\u8fd8\u53ef\u4ee5\u8fdb\u4e00\u6b65\u5e94\u7528\u4e8e\u5bf9\u6784\u578b\u9009\u62e9\u6027\u7684\u5316\u5408\u7269\u4fee\u9970\u8fdb\u884c\u9274\u5b9a\u3002\u4f5c\u8005\u5229\u7528rdTOP-ABPP\u9274\u5b9a\u7ec6\u80de\u94c1\u6b7b\u4ea1\u8bf1\u5bfc\u5242RSL3\u5bf9\u534a\u80f1\u6c28\u9178\u7684\u4fee\u9970\u5bf9\u8fd9\u4e00\u65b9\u9762\u7684\u5e94\u7528\u8fdb\u884c\u4e86\u5c55\u793a\u3002\u94c1\u6b7b\u4ea1\u662f\u57282012\u5e74\u5b9a\u4e49\u7684\u4e00\u79cd\u65b0\u7684\u7ec6\u80de\u6b7b\u4ea1\u65b9\u5f0f\uff0c\u8fd9\u79cd\u6b7b\u4ea1\u65b9\u5f0f\u4e3b\u8981\u4f9d\u8d56\u4e8e\u94c1\u4ee3\u8c22\u548cROS\u4ee3\u8c22\u901a\u8def\u7684\u8c03\u8282\uff0c\u5728\u7ec6\u80de\u5f62\u6001\u5b66\u3001\u751f\u5316\u7279\u5f81\u7b49\u65b9\u9762\u4e0e\u5176\u4ed6\u6b7b\u4ea1\u65b9\u5f0f\u90fd\u5b58\u5728\u7740\u663e\u8457\u5dee\u5f02\u3002Stockwell\u7ec4\u901a\u8fc7\u9ad8\u901a\u91cf\u7b5b\u9009\u7684\u65b9\u5f0f\u627e\u5230\u4e86\u5316\u5b66\u5c0f\u5206\u5b50RSL3\u53ef\u4ee5\u8bf1\u5bfc\u94c1\u6b7b\u4ea1\uff0c\u540e\u7eed\u7684\u5b9e\u9a8c\u8868\u660e\u53ea\u6709\uff081S<\/em>, 3R<\/em>\uff09\u6784\u578b\u6709\u8bf1\u5bfc\u94c1\u6b7b\u4ea1\u7684\u6d3b\u6027\uff0c\u800c\uff081R<\/em>, 3R<\/em>\uff09\u6784\u578b\u7684\u6d3b\u6027\u53ea\u6709\uff081S<\/em>, 3R<\/em>\uff09\u6d3b\u6027\u76841\/200\u3002\u57fa\u4e8e\u8fd9\u4e00\u73b0\u8c61\uff0c\u4f5c\u8005\u4f7f\u7528rdTOP-ABPP\u6280\u672f\u4e00\u6b21\u6027\u5b9a\u91cf\u6bd4\u8f83\u4e86DMSO\u3001(1S<\/em>, 3R<\/em>)-RSL3\u3001(1R<\/em>, 3R<\/em>)-RSL3\u5bf9\u86cb\u767d\u8d28\u7ec4\u4e2d\u534a\u80f1\u6c28\u9178\u7684\u6807\u8bb0\u60c5\u51b5\uff0c\u5e76\u53d1\u73b0\u4e86\u4e00\u4e2a\u88ab\uff081S<\/em>,3R<\/em>\uff09\u6784\u578bRSL3\u7279\u5f02\u6027\u4fee\u9970\u7684\u9ad8\u7f6e\u4fe1\u5ea6\u4f4d\u70b9\u4e3aVDAC2\u768447\u4f4d\u534a\u80f1\u6c28\u9178\u3002<\/span><\/p>\n


<\/span><\/p>\n

\u7531\u4e8erdTOP-ABPP\u65b9\u6cd5\u7b80\u5355\u9ad8\u6548\uff0c\u5e76\u4e14\u4e0e\u591a\u79cd\u5546\u4e1a\u5316\u53ef\u5207\u5272\u7684\u751f\u7269\u7d20\u63a2\u9488\u517c\u5bb9\uff0c\u56e0\u6b64\u5177\u6709\u5f88\u597d\u666e\u9002\u6027\u548c\u53ef\u63a8\u5e7f\u6027\u3002\u8fd9\u79cd\u65b0\u65b9\u6cd5\u80fd\u591f\u4e3a\u53d1\u73b0\u548c\u63cf\u8ff0\u590d\u6742\u751f\u7269\u7cfb\u7edf\u4e2d\u86cb\u767d\u8d28\u914d\u4f53\u7684\u76f8\u4e92\u4f5c\u7528\u3001\u836f\u7269\u9776\u6807\u53d1\u73b0\u548c\u86cb\u767d\u8d28\u7ffb\u8bd1\u540e\u4fee\u9970\u7b49\u65b9\u9762\u7684\u7814\u7a76\u63d0\u4f9b\u65b0\u7684\u6280\u672f\u9009\u62e9\u3002<\/span><\/p>\n


<\/span><\/p>\n

\u8fd9\u4e00\u6210\u679c\u8fd1\u671f\u53d1\u8868\u5728Analytical Chemistry<\/em><\/strong>\u4e0a\uff0c\u6587\u7ae0\u7684\u7b2c\u4e00\u4f5c\u8005\u662f\u5317\u4eac\u5927\u5b66\u7684\u535a\u58eb\u7814\u7a76\u751f\u6768\u5e06<\/strong>\u548c\u9ad8\u664b\u541b<\/strong>\u3002\u5317\u4eac\u5927\u5b66\u7f57\u9640\u5e73<\/strong>\u8bfe\u9898\u7ec4\u7684\u535a\u58eb\u7814\u7a76\u751f\u8f66\u91d1\u817e<\/strong>\u3001\u738b\u521d<\/strong>\u8bfe\u9898\u7ec4\u7684\u672c\u79d1\u751f\u8d3e\u56fd\u8d53<\/strong>\u4e3a\u8be5\u8bfe\u9898\u7684\u5b8c\u6210\u63d0\u4f9b\u4e86\u5e2e\u52a9\u3002<\/span><\/p>\n


<\/p>\n

\u8be5\u8bba\u6587\u4f5c\u8005\u4e3a\uff1a<\/span><\/strong>Fan Yang, Jinjun Gao, Jinteng Che, Guogeng Jia, Chu Wang<\/span><\/p>\n

\u539f\u6587\uff08\u626b\u63cf\u6216\u957f\u6309\u4e8c\u7ef4\u7801\uff0c\u8bc6\u522b\u540e\u76f4\u8fbe\u539f\u6587\u9875\u9762\uff09\uff1a<\/span><\/p>\n

\"\u3010\u5206\u6790\u3011\u5b9a\u91cf\u5316\u5b66\u86cb\u767d\u8d28\u7ec4\u5b66\u65b0\u7b56\u7565\"<\/p>\n

A Dimethyl-Labeling-Based Strategy for Site-Specifically Quantitative Chemical Proteomics<\/span><\/p>\n

Anal. Chem<\/span><\/em>., 2018<\/strong>, 90<\/em>, 9576, DOI: 10.1021\/acs.analchem.8b02426<\/span><\/p>\n


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\u5bfc\u5e08\u4ecb\u7ecd<\/span><\/p>\n

\u738b\u521d<\/span><\/p>\n

http:\/\/www.x-mol.com\/university\/faculty\/8705<\/span><\/p>\n


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<\/a>\"\u3010\u5206\u6790\u3011\u5b9a\u91cf\u5316\u5b66\u86cb\u767d\u8d28\u7ec4\u5b66\u65b0\u7b56\u7565\"<\/span><\/a><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n


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\"\u3010\u5206\u6790\u3011\u5b9a\u91cf\u5316\u5b66\u86cb\u767d\u8d28\u7ec4\u5b66\u65b0\u7b56\u7565\"<\/p>\n

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