{"id":85966,"date":"2019-04-15T00:00:50","date_gmt":"2019-04-18T07:54:15","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/85966\/"},"modified":"2019-04-18T15:54:17","modified_gmt":"2019-04-18T07:54:17","slug":"404aae2b07","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/85966\/","title":{"rendered":"\u80bf\u7624\u5fae\u73af\u5883\u5206\u6790\u6307\u5bfc\u764c\u75c7\u6cbb\u7597"},"content":{"rendered":"
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\"\u80bf\u7624\u5fae\u73af\u5883\u5206\u6790\u6307\u5bfc\u764c\u75c7\u6cbb\u7597\"<\/span><\/p>\n

\u4e73\u817a\u764c\u80bf\u7624\u5468\u56f4\u7684\u5fae\u73af\u5883\uff08\u84dd\u8272\uff09\u4e2d\u5b58\u5728\u7740\u4e0d\u540c\u7c7b\u578b\u7684\u514d\u75ab\u7ec6\u80de\uff0c\u6bd4\u5982T\u7ec6\u80de\u548cB\u7ec6\u80de\u3002<\/span><\/p>\n

\u5728\u514d\u75ab\u7597\u6cd5\u65f6\u4ee3\uff0c\u764c\u75c7\u751f\u7269\u5b66\u5bb6\u4f9d\u9760\u65b0\u4e00\u4ee3\u5de5\u5177\u6765\u4e86\u89e3\u80bf\u7624\u548c\u514d\u75ab\u7ec6\u80de\u4e4b\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u662f\u5982\u4f55\u5f71\u54cd\u75be\u75c5\u8fdb\u7a0b\u7684\u3002<\/strong><\/span><\/p>\n

\u867d\u7136Sean Bendall\u662f\u4e00\u540d\u75c5\u7406\u5b66\u5bb6\uff0c\u4f46\u6700\u8fd1\u5374\u6210\u4e86\u4e00\u540d\u56fe\u8c31\u5236\u4f5c\u8005\uff0c\u4ed6\u4f7f\u7528\u5c16\u7aef\u7684\u86cb\u767d\u8d28\u7ed8\u56fe\u6280\u672f\u6765\u63cf\u7ed8\u51fa\u80bf\u7624\u7ec4\u7ec7\u7684\u53d8\u5e7b\u83ab\u6d4b\u7684\u666f\u89c2\u3002<\/span><\/p>\n

\u8fd9\u9879\u6280\u672f\u7531Bendall\u5728\u52a0\u5dde\u65af\u5766\u798f\u5927\u5b66\uff08Stanford University\uff09\u7684\u540c\u4e8bMichael Angelo\u5f00\u53d1\u3002\u8be5\u6280\u672f\u80fd\u9488\u5bf9\u764c\u7ec6\u80de\u53ca\u5176\u8fd1\u90bb\u533a\u57df\u8fdb\u884c\u7ec6\u81f4\u7684\u5206\u5b50\u8c31\u7ed8\u5236\u2014\u2014\u6700\u8457\u540d\u7684\u5e94\u7528\u662f\u6d4b\u7ed8\u80bf\u7624\u5185\u5916\u7684\u514d\u75ab\u7ec6\u80de\u3002Bendall\u5f97\u5230\u7684\u4e00\u4e9b\u8bc1\u636e\u8868\u660e\uff0c\u8fd9\u4e9b\u56fe\u8c31\u53ef\u4ee5\u5e2e\u52a9\u4e34\u5e8a\u533b\u751f\u4e3a\u67d0\u4e9b\u75c5\u4eba\u9009\u62e9\u5408\u9002\u7684\u6cbb\u7597\u65b9\u6848\u3002\u57282018\u5e74\u7684\u4e00\u9879\u7814\u7a76\u4e2d\uff0cBendall\u548cAngelo\u4f7f\u7528\u4ed6\u4eec\u7684\u6280\u672f\uff08\u88ab\u79f0\u4e3a\u591a\u91cd\u79bb\u5b50\u675f\u6210\u50cf\uff08multiplexed ion beam imaging, MIBI\uff09\u68c0\u6d4b\u66fe\u63a5\u53d7\u4e09\u9634\u6027\u4e73\u817a\u764c\uff08triple-negative breast cancer\uff0c\u4e00\u79cd\u7279\u522b\u5177\u6709\u653b\u51fb\u6027\u7684\u80bf\u7624\u7c7b\u578b\uff09\u6807\u51c6\u5316\u7597\u7684\u75c5\u4eba\u7684\u80bf\u7624\u6807\u672c\u7684\u7ed3\u6784\u6027\u80bf\u7624\u514d\u75ab\u5fae\u73af\u5883\u3002\u7ed3\u679c\uff0cBendall\u7b49\u4eba\u53d1\u73b0\u4e86\u4e00\u79cd\u80fd\u591f\u9ad8\u5ea6\u9884\u6d4b\u957f\u671f\u65e0\u75c5\u751f\u5b58\u7684\u514d\u75ab\u5fae\u73af\u5883\u7c7b\u578b\u3002<\/span><\/p>\n

\u4e00\u4e2a\u5e38\u89c1\u7684\u8bef\u89e3\u662f\uff0c\u80bf\u7624\u7ec4\u7ec7\u7531\u4e24\u79cd\u7ec6\u80de\u7ec4\u6210\uff0c\u5373\u7531\u5f02\u5e38\u6216\u5065\u5eb7\u7684\u7ec6\u80de\u7ec4\u6210\u3002\u4f46\u73b0\u5b9e\u8fdc\u6bd4\u8fd9\u4e2a\u590d\u6742\uff1a\u764c\u7ec6\u80de\u4e0e\u514d\u75ab\u7ec6\u80de\u3001\u8840\u7ba1\u548c\u652f\u6301\u7ed3\u7f14\u7ec4\u7ec7\u5e7f\u6cdb\u76f8\u4e92\u4f5c\u7528\u3002\u8fd9\u79cd\u80bf\u7624\u7684\u201c\u5fae\u73af\u5883\u201d\u53ef\u4ee5\u6df1\u523b\u5730\u5f71\u54cd\u75be\u75c5\u7684\u7279\u5f81\u548c\u60a3\u8005\u5bf9\u6cbb\u7597\uff0c\u7279\u522b\u662f\u514d\u75ab\u7597\u6cd5\u7684\u53cd\u5e94\uff0c\u514d\u75ab\u7597\u6cd5\u662f\u4e00\u79cd\u53ef\u4ee5\u63d0\u9ad8\u673a\u4f53\u514d\u75ab\u7cfb\u7edf\u5bf9\u80bf\u7624\u6740\u4f24\u4f5c\u7528\u7684\u6cbb\u7597\u65b9\u6cd5\u3002\u4f8b\u5982\uff0c\u5fae\u73af\u5883\u53ef\u4ee5\u51b3\u5b9a\u9644\u8fd1\u7684\u514d\u75ab\u7ec6\u80de\u662f\u5426\u201c\u5f00\u542f\u201d\u6216\u201c\u5173\u95ed\u201d\uff0c\u6216\u8005\u751a\u81f3\u662f\u5426\u80fd\u591f\u63a5\u8fd1\u80bf\u7624\u3002\u6b63\u5982\u7ebd\u7ea6\u7eaa\u5ff5\u65af\u9686-\u51ef\u7279\u7433\u764c\u75c7\u4e2d\u5fc3\uff08Memorial Sloan-Kettering Cancer Center\uff09\u7684\u8ba1\u7b97\u751f\u7269\u5b66\u5bb6Dana Pe’er\u6240\u8bf4\uff0c \u80bf\u7624\u4e0d\u4ec5\u4ec5\u662f\u4e00\u5806\u540c\u8d28\u7ec6\u80de\uff0c\u5b83\u5b9e\u9645\u4e0a\u662f\u4e00\u4e2a\u5668\u5b98\u2014\u2014\u4e00\u79cd\u4e25\u91cd\u7578\u5f62\u7684\u5668\u5b98\u3002<\/span><\/p>\n

\u5229\u7528MIBI\u4e4b\u7c7b\u7684\u80bf\u7624\u7ed8\u56fe\u6280\u672f\uff0c\u4ee5\u53ca\u57fa\u4e8e\u57fa\u56e0\u8868\u8fbe\u6216\u86cb\u767d\u8d28\u542b\u91cf\u5bf9\u5927\u91cf\u5355\u4e2a\u7ec6\u80de\u8fdb\u884c\u8be6\u7ec6\u666e\u67e5\u7684\u5176\u5b83\u65b9\u6cd5\uff0c\u7814\u7a76\u4eba\u5458\u73b0\u5728\u6b63\u5728\u5256\u6790\u80bf\u7624\u5fae\u73af\u5883\u7684\u7ed3\u6784\u548c\u529f\u80fd\u3002\u8fd9\u4e9b\u5de5\u5177\u662f\u5982\u6b64\u65b0\u9896\uff0c\u4ee5\u81f4\u8bb8\u591a\u7814\u7a76\u4eba\u5458\u4ecd\u7136\u5728\u6478\u7d22\u5982\u4f55\u80fd\u6700\u4f73\u5730\u5229\u7528\u8fd9\u4e9b\u5de5\u5177\u3002\u4f46\u7531\u6b64\u4ea7\u751f\u7684\u89c1\u89e3\u53ef\u80fd\u6709\u52a9\u4e8e\u5f00\u521b\u4e00\u4e2a\u65b0\u7684\u80bf\u7624\u5256\u6790\u65f6\u4ee3\u2014\u2014\u4e00\u4e2a\u901a\u8fc7\u6df1\u5ea6\u5206\u6790\u80bf\u7624\u5fae\u73af\u5883\uff0c\u6536\u96c6\u4f8b\u5982\u5176\u4e2d\u5b58\u5728\u7684\u514d\u75ab\u7ec6\u80de\u7684\u6570\u91cf\u548c\u7c7b\u578b\u4e4b\u7c7b\u7684\u4fe1\u606f\uff0c\u800c\u4e0d\u662f\u4e13\u6ce8\u4e8e\u4e2a\u4f53\u9057\u4f20\u53d8\u5f02\uff0c\u6700\u7ec8\u5f97\u5230\u7ec6\u80de\u751f\u6001\u7cfb\u7edf\u7684\u201c\u5168\u5c40\u201d\u89c6\u56fe\u7684\u65f6\u4ee3\u3002<\/span><\/p>\n

\u7ebd\u7ea6\u5e02\u897f\u5948\u5c71\u4f0a\u574e\u533b\u5b66\u9662\uff08Icahn School of Medicine\uff09\u7684\u80bf\u7624\u5185\u79d1\u533b\u751fMiriam Merad\u8868\u793a\uff0c\u8fd9\u4e9b\u529f\u80fd\u786e\u5b9e\u4f1a\u5bf9\u764c\u75c7\u6cbb\u7597\u4ea7\u751f\u5f71\u54cd\u3002<\/span><\/p>\n


<\/p>\n

\u7ec6\u80de\u666e\u67e5<\/strong>
<\/span><\/h3>\n

\u80bf\u7624\u548c\u514d\u75ab\u7cfb\u7edf\u4e4b\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u53ef\u80fd\u4f1a\u5f71\u54cd\u4eba\u7c7b\u75be\u75c5\uff0c\u8fd9\u4e2a\u89c2\u70b9\u5e76\u4e0d\u65b0\u9c9c\u2014\u201450\u5e74\u6765\uff0c\u514d\u75ab\u5b66\u5bb6Wolf Fridman\u57fa\u4e8e\u5bf9\u767d\u8840\u75c5\u4e2d\u540d\u4e3aT\u7ec6\u80de\u7684\u514d\u75ab\u7ec6\u80de\u53cd\u5e94\u7684\u65e9\u671f\u89c2\u5bdf\uff0c\u4e00\u76f4\u5728\u7814\u7a76\u8fd9\u79cd\u76f8\u4e92\u4f5c\u7528\u3002Fridman\u6307\u51fa\uff0c\u5f88\u5feb\u4ed6\u5c31\u786e\u4fe1\u514d\u75ab\u53cd\u5e94\u7684\u4f4d\u7f6e\u548c\u7ec4\u7ec7\u975e\u5e38\u91cd\u8981\u3002Fridman\u73b0\u5728\u662f\u5df4\u9ece\u7b1b\u5361\u5c14\u533b\u5b66\u9662\uff08Medical School Paris Descartes\uff09\u7684\u540d\u8a89\u6559\u6388\u3002\u7136\u800c\uff0c\u76f4\u5230\u5927\u7ea6\u5341\u5e74\u524d\uff0c\u968f\u7740\u5f3a\u5927\u7684\u514d\u75ab\u6cbb\u7597\u7b56\u7565\u7684\u51fa\u73b0\uff0c\u8fd9\u4e9b\u76f8\u4e92\u4f5c\u7528\u7684\u5e7f\u6cdb\u5f71\u54cd\u624d\u6210\u4e3a\u80bf\u7624\u5b66\u5bb6\u7684\u4e3b\u8981\u7126\u70b9\u3002<\/span><\/p>\n

\u75c5\u7406\u5b66\u5bb6\u53ef\u4ee5\u7528\u82cf\u6728\u7cbe\u548c\u4f0a\u7ea2\uff08haematoxylin and eosin, H\uff06E\uff09\u67d3\u8272\u548c\u514d\u75ab\u7ec4\u7ec7\u5316\u5b66\uff08immunohistochemistry, IHC\uff09\u7b49\u5e38\u89c4\u6280\u672f\u83b7\u5f97\u80bf\u7624\u514d\u75ab\u7ec6\u80de\u7ec4\u6210\u7684\u4fe1\u606f\u3002IHC\u4f7f\u7528\u8bc6\u522b\u7ec4\u7ec7\u6807\u672c\u4e2d\u7279\u5b9a\u5206\u5b50\u7279\u5f81\u7684\u9176\u6807\u8bb0\u6297\u4f53\uff0c\u5df2\u88ab\u8bc1\u660e\u80fd\u4e3a\u68c0\u67e5\u70b9\u6291\u5236\u5242\u7c7b\u836f\u7269\u7684\u4f7f\u7528\u63d0\u4f9b\u91cd\u8981\u6307\u5bfc\u4fe1\u606f\u3002\u514d\u75ab\u68c0\u67e5\u70b9\u6cbb\u7597\u5242\u7684\u4f5c\u7528\u673a\u5236\u662f\u963b\u65ad\u90a3\u4e9b\u6291\u5236\u5c40\u90e8\u514d\u75ab\u7ec6\u80de\u653b\u51fb\u80bf\u7624\u7684\u7279\u5f02\u6027\u4fe1\u53f7\u4f20\u5bfc\u86cb\u767d\uff0c\u800cIHC\u53ef\u4ee5\u63ed\u793a\u8fd9\u4e9b\u86cb\u767d\u8d28\u7684\u5b58\u5728\u3002Bendall\u63d0\u9192\uff0c\u5982\u679c\u60a3\u8005\u80ba\u90e8\u80bf\u7624\u4e2d\u68c0\u67e5\u70b9\u86cb\u767d\u8d28\u8868\u8fbe\u5f88\u4f4e\uff0c\u90a3\u4e48\u4f7f\u7528\u68c0\u67e5\u70b9\u6291\u5236\u5242\u5c31\u57fa\u672c\u4e0d\u4f1a\u6709\u6548\u679c\u3002\u7136\u800c\uff0c\u8bb8\u591a\u8868\u8fbe\u8fd9\u4e9b\u86cb\u767d\u8d28\u7684\u60a3\u8005\u5bf9\u68c0\u67e5\u70b9\u6291\u5236\u5242\u4e5f\u6ca1\u6709\u54cd\u5e94\uff0c\u7814\u7a76\u4eba\u5458\u6b63\u5728\u5bfb\u627e\u53ef\u80fd\u66f4\u6e05\u695a\u5730\u9884\u6d4b\u6cbb\u7597\u662f\u5426\u6709\u6548\u7684\u5176\u5b83\u75ab\u7279\u5f81\u3002\u4f46IHC\u5e76\u4e0d\u662f\u53d1\u73b0\u8fd9\u4e9b\u7279\u5f81\u7684\u6700\u4f73\u7b56\u7565\uff0c\u56e0\u4e3a\u5b83\u4e00\u6b21\u53ea\u80fd\u5206\u6790\u5c11\u6570\u5206\u5b50\u6807\u8bb0\u3002<\/span><\/p>\n

\"\u80bf\u7624\u5fae\u73af\u5883\u5206\u6790\u6307\u5bfc\u764c\u75c7\u6cbb\u7597\"<\/span><\/p>\n

MIBI\u5f97\u5230\u7684\u80bf\u7624\u548c\u514d\u75ab\u7ec6\u80de\u7684\u5206\u5b50\u8c31\u3002\u7ec6\u80de\u88ab\u67d3\u4e0a\u8272\uff0c\u4ee5\u663e\u793a\u5176\u8ddd\u80bf\u7624\u8fb9\u754c\u7684\u8ddd\u79bb\uff08\u5de6\uff09\u6216\u9057\u4f20\u6807\u8bb0\u7684\u8868\u8fbe\uff08\u53f3\uff09\u3002<\/span><\/p>\n

\u98de\u884c\u65f6\u95f4\u8d28\u8c31\u6d41\u5f0f\u7ec6\u80de\u672f\uff08Mass cytometry by time-of-flight, CyTOF\uff09\u53ef\u4ee5\u5bf9\u66f4\u591a\u7684\u80bf\u7624\u7ec6\u80de\u8fdb\u884c\u5206\u6790\u3002\u5e38\u89c4\u6d41\u5f0f\u7ec6\u80de\u672f\u5c06\u6297\u4f53\u5076\u8054\u5230\u67d3\u6599\u6216\u8367\u5149\u6807\u8bb0\u4e0a\uff0c\u800cCyTOF\u4f7f\u7528\u4e0e\u91d1\u5c5e\u540c\u4f4d\u7d20\u8fde\u63a5\u7684\u6297\u4f53\u6765\u6807\u8bb0\u6765\u81ea\u80bf\u7624\u6837\u672c\u7684\u5927\u91cf\u7ec6\u80de\u3002\u8d28\u8c31\u4eea\u80fd\u591f\u975e\u5e38\u5feb\u901f\u5730\u5206\u6790\u8fd9\u4e9b\u540c\u4f4d\u7d20\u6807\u7b7e\uff0c\u5e76\u884c\u5730\u4e3a\u6bcf\u4e2a\u7ec6\u80de\u68c0\u6d4b\u548c\u91cf\u5316\u6570\u5341\u79cd\u4e0d\u540c\u7684\u6807\u8bb0\u7269\u3002<\/span><\/p>\n

2017\u5e74\uff0cMerad\u7b49\u4eba\u5c06CyTOF\u5e94\u7528\u4e8e\u80ba\u817a\u764c\uff08lung adenocarcinoma\uff09\u2014\u2014\u4e00\u79cd\u6700\u5e38\u89c1\u7684\u80ba\u764c\u5f62\u5f0f\uff0c\u5e76\u4f7f\u7528\u591a\u8fbe40\u79cd\u4e0d\u540c\u7684\u6807\u7b7e\u6297\u4f53\u6765\u6807\u8bb0\u7ec6\u80de\u3002\u4ed6\u4eec\u7684\u6570\u636e\u63ed\u793a\u4e86\u65b0\u51fa\u73b0\u7684\u80bf\u7624\u5982\u4f55\u901a\u8fc7\u62db\u52df\u514d\u75ab\u6291\u5236\u8c03\u8282\u6027T\u7ec6\u80de\uff0c\u5e76\u963b\u6b62\u5305\u62ec\u6740\u6b7b\u80bf\u7624\u7684\u81ea\u7136\u6740\u4f24\u7ec6\u80de\u5728\u5185\u7684\u201c\u6548\u5e94\u7ec6\u80de\u201d\u7684\u8fc1\u79fb\u6765\u8fc5\u901f\u6291\u5236\u75c5\u4eba\u7684\u514d\u75ab\u53cd\u5e94\u3002Merad\u6307\u51fa\uff0c\u5373\u4f7f\u5728\u80bf\u7624\u5f00\u59cb\u53d8\u5f97\u6076\u6027\u7684\u65e9\u671f\u9636\u6bb5\uff0c\u80bf\u7624\u7ec6\u80de\u90fd\u4f1a\u60f3\u529e\u6cd5\u6291\u5236\u8fd9\u4e9b\u6548\u5e94\u7ec6\u80de\u3002\u8fd9\u4e9b\u7ed3\u679c\u8868\u660e\uff0c\u5728\u80bf\u7624\u53d1\u5c55\u7684\u65e9\u671f\u9006\u8f6c\u514d\u75ab\u6291\u5236\u53ef\u80fd\u4f1a\u963b\u6b62\u80bf\u7624\u8fdb\u5c55\uff0c\u5e76\u6709\u671b\u963b\u6b62\u8f6c\u79fb\u3002<\/span><\/p>\n


<\/p>\n

\u5206\u6790\u8f6c\u5f55\u7269<\/strong>
<\/span><\/h3>\n

CyTOF\u9700\u8981\u4e8b\u5148\u4e86\u89e3\u7ec6\u80de\u7c7b\u578b\u7279\u5f02\u6027\u6807\u8bb0\uff0c\u4ee5\u4fbf\u7814\u7a76\u4eba\u5458\u9009\u62e9\u5408\u9002\u7684\u6297\u4f53\u3002Peer\u6307\u51fa\uff0cCyTOF\u7684\u4e00\u4e2a\u5c40\u9650\u6027\u662f\uff0c\u51e0\u4e4e\u6ca1\u6709\u63d0\u4f9b\u6709\u5173\u751f\u7269\u529f\u80fd\u7684\u76f4\u63a5\u4fe1\u606f\uff0c\u53ea\u63d0\u793a\u7ec6\u80de\u6807\u8bb0\u7684\u5b58\u5728\u4e0e\u5426\u3002<\/span><\/p>\n

\u8bf8\u5982RNA\u6d4b\u5e8f\uff08RNA sequencing, RNA-seq\uff09\u7684\u8f6c\u5f55\u7ec4\u5206\u6790\u6280\u672f\u53ef\u4ee5\u63d0\u793a\u4e00\u4e9b\u529f\u80fd\u65b9\u9762\u7684\u4fe1\u606f\u3002RNA-seq\u91c7\u7528\u9ad8\u901a\u91cf\u6d4b\u5e8f\u5e73\u53f0\u6765\u8868\u5f81\u548c\u5b9a\u91cf\u5927\u91cf\u86cb\u767d\u8d28\u7f16\u7801\u4fe1\u4f7fRNA\u8f6c\u5f55\u672c\u3002\u8fd9\u63d0\u4f9b\u4e86\u4e00\u4e2a\u76f4\u63a5\u7684\u7a97\u53e3\uff0c\u53ef\u7528\u4e8e\u89c2\u6d4b\u54ea\u4e9b\u57fa\u56e0\u5728\u7ed9\u5b9a\u7684\u7ec6\u80de\u4e2d\u88ab\u5173\u95ed\u548c\u6253\u5f00\uff0c\u4ece\u800c\u63ed\u793a\u80bf\u7624\u4e2d\u53d1\u751f\u7684\u751f\u7269\u6d3b\u52a8\u3002\u5341\u591a\u5e74\u6765\uff0c\u7814\u7a76\u4eba\u5458\u4e00\u76f4\u5728\u5e94\u7528\u8fd9\u4e9b\u6280\u672f\uff0c\u4f46\u5176\u4e2d\u5927\u90e8\u5206\u90fd\u662f\u201c\u5927\u91cf\u201d\u5206\u6790\u2014\u2014\u4e00\u6b21\u6027\u5206\u6790\u80bf\u7624\u4e2d\u7684\u6bcf\u4e2a\u7ec6\u80de\u3002\u9a6c\u8428\u8bf8\u585e\u5dde\u5251\u6865\u5e02\u535a\u5fb7\u7814\u7a76\u6240\uff08Broad Institute\uff09\u7684\u8ba1\u7b97\u751f\u7269\u5b66\u5bb6Aviv Regev\u6307\u51fa\uff0c\u867d\u7136\u8fd9\u79cd\u65b9\u6cd5\u53ef\u4ee5\u63d0\u4f9b\u6709\u7528\u7684\u89c1\u89e3\uff0c\u4f46\u4e5f\u53ef\u63a9\u76d6\u4e86\u7ec6\u80de\u4e4b\u95f4\u7684\u5dee\u5f02\u3002<\/span><\/p>\n

Regev\u7684\u5b9e\u9a8c\u5ba4\u5f00\u53d1\u4e86\u4e00\u79cd\u540d\u4e3aDrop-seq\u7684\u65b9\u6cd5\uff0c\u8be5\u65b9\u6cd5\u5c06\u5355\u4e2a\u7ec6\u80de\u5c01\u88c5\u5728\u5355\u4e2a\u8102\u6ef4\u4e2d\uff0c\u7ecf\u9884\u5904\u7406\u540e\u4fbf\u53ef\u8fdb\u884c\u6d4b\u5e8f\u3002\u5728\u6b64\u8fc7\u7a0b\u4e2d\uff0c\u6bcf\u4e2a\u7ec6\u80de\u7684RNA\u90fd\u4f1a\u6709\u4e00\u4e2a\u4e0d\u540c\u7684\u9057\u4f20\u6761\u5f62\u7801\uff0c\u4ece\u800c\u76f4\u63a5\u786e\u5b9a\u54ea\u4e9b\u8f6c\u5f55\u7269\u6765\u81ea\u540c\u4e00\u7ec6\u80de\u3002Regev\u8868\u793a\uff0c\u4ed6\u4eec\u5f97\u5230\u7684\u7ed3\u679c\u975e\u5e38\u5168\u9762\u2014\u2014\u4e30\u5bcc\u7684\u7ec6\u80de\u7c7b\u578b\uff0c\u4ee5\u53ca\u4e30\u5bcc\u7684\u5206\u5b50\u79cd\u7c7b\u3002\u81f3\u5173\u91cd\u8981\u7684\u662f\uff0c\u548c\u5176\u5b83\u5355\u7ec6\u80deRNA-seq\u65b9\u6cd5\u4e0d\u540c\uff0cDrop-seq\u4e0d\u9700\u8981\u4e8b\u5148\u77e5\u9053\u611f\u5174\u8da3\u7684\u57fa\u56e0\u3001\u57fa\u56e0\u8868\u8fbe\u8c31\uff0c\u4ee5\u53ca\u53ef\u4ee5\u7528\u4e8e\u91cd\u5efa\u7ec6\u80de\u4e4b\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u3002Regev\u53bb\u5e74\u9488\u5bf9\u9ed1\u7d20\u7624\u80bf\u7624\u7684\u7814\u7a76\u4e2d\u8bc1\u5b9e\u4e86Drop-seq\u6280\u672f\u7684\u8fd9\u79cd\u72ec\u7279\u4f18\u52bf\u3002Regev\u7b49\u4eba\u53d1\u73b0\u6076\u6027\u7ec6\u80de\u5b9e\u9645\u4e0a\u53ef\u4ee5\u5448\u73b0\u4e00\u79cd\u7279\u5b9a\u7684\u72b6\u6001\uff0c\u5b83\u4eec\u5f62\u6210\u6240\u8c13\u7684\u2018\u51b7\u7684\u751f\u6001\u4f4d\u2019\uff08cold niches\uff09\uff0c\u90a3\u91cc\u6ca1\u6709T\u7ec6\u80de\u3002\u53ef\u60f3\u800c\u77e5\uff0c\u8fd9\u6837\u7684\u80bf\u7624\u533a\u57df\u5bf9\u514d\u75ab\u7597\u6cd5\u66f4\u5177\u6297\u6027\u3002<\/span><\/p>\n

\u5355\u7ec6\u80deRNA-seq\uff08scRNA-seq\uff09\u7684\u5b9e\u65bd\u76f8\u5bf9\u7b80\u5355\uff0c\u76ee\u524d\u6709\u51e0\u79cd\u5546\u4e1a\u4eea\u5668\u53ef\u4f9b\u4f7f\u7528\u3002\u4f46\u8be5\u6280\u672f\u4ecd\u7136\u5b58\u5728\u4e00\u4e9b\u91cd\u8981\u6311\u6218\u3002\u4e5f\u8bb8\u6700\u91cd\u8981\u7684\u662f\u80bf\u7624\u6807\u672c\u5fc5\u987b\u76f4\u63a5\u4ece\u624b\u672f\u5ba4\u8fd0\u8f93\u5230\u5b9e\u9a8c\u5ba4\uff0c\u4e2d\u95f4\u7684\u6d41\u8f6c\u65f6\u95f4\u5fc5\u987b\u77ed\u3002Merad\u6307\u51fa\uff0c\u5355\u7ec6\u80de\u65b9\u6cd5\u4ec5\u5bf9\u65b0\u9c9c\u7ec6\u80de\u63d0\u4f9b\u4fe1\u606f\uff0c\u56e0\u4e3a\u5f53\u6211\u4eec\u51b7\u51bb\u548c\u89e3\u51bb\u7ec6\u80de\u65f6RNA\u5f88\u5feb\u5c31\u4f1a\u964d\u89e3\u3002<\/span><\/p>\n

\u7136\u800c\uff0c\u8be5\u6280\u672f\u53ef\u4ee5\u63ed\u793a\u5176\u5b83\u65b9\u6cd5\u65e0\u6cd5\u63ed\u793a\u7684\u7ec6\u80de\u7c7b\u578b\u548c\u7ec6\u80de\u72b6\u6001\uff0c\u5e76\u63ed\u793a\u514d\u75ab\u7ec6\u80de\u529f\u80fd\u7684\u7ec6\u5fae\u5dee\u522b\uff0c\u4f20\u7edf\u7528\u4e8e\u5206\u7c7b\u7ec6\u80de\u7684\u7b80\u5355\u7c7b\u522b\u5b8c\u5168\u4e0d\u8db3\u4ee5\u6db5\u76d6\u8fd9\u4e9b\u7ec6\u5fae\u5dee\u522b\u3002Pe’er\u8868\u793a\uff0c\u514d\u75ab\u7ec6\u80de\u4e4b\u95f4\u7684\u5dee\u5f02\u548c\u5b50\u7c7b\u578b\u8fdc\u6bd4\u6211\u4eec\u60f3\u7684\u8981\u591a\uff0c\u6211\u4eec\u8fc7\u53bb\u5e38\u559c\u6b22\u6839\u636e\u7ec6\u80de\u662f\u4fc3\u764c\u8fd8\u662f\u6291\u764c\uff0c\u5c06\u5176\u5206\u7c7b\u4e3a\u597d\u7684\u548c\u574f\u7684\uff0c\u4f46\u7cfb\u7edf\u8981\u590d\u6742\u5f97\u591a\uff0c\u5927\u591a\u6570\u7ec6\u80de\u65e2\u6709\u597d\u7684\u4e00\u9762\uff0c\u53c8\u6709\u574f\u7684\u4e00\u9762\u3002<\/span><\/p>\n


<\/p>\n

\u5404\u79cd\u6280\u672f\u7684\u5229\u4e0e\u5f0a<\/strong>
<\/span><\/h3>\n

\u5c3d\u7ba1CyTOF\u548cRNA-seq\u529f\u80fd\u5f3a\u5927\uff0c\u4f46\u662f\u8fd8\u9700\u8981\u8fdb\u884c\u4e25\u683c\u7684\u6743\u8861\uff0c\u56e0\u4e3a\u5b83\u4eec\u9700\u8981\u7834\u574f\u80bf\u7624\u7ec4\u7ec7\u624d\u80fd\u5f97\u5230\u5355\u4e2a\u7ec6\u80de\u3002\u7834\u574f\u7ec4\u7ec7\u610f\u5473\u7740\u4e22\u5931\u7a7a\u95f4\u7ec6\u8282\uff0c\u800c\u8fd9\u4e9b\u7ec6\u8282\u5bf9\u4e8e\u7406\u89e3\u6076\u6027\u7ec6\u80de\u4e0e\u5176\u5fae\u73af\u5883\u4e4b\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u975e\u5e38\u91cd\u8981\u3002\u8bf8\u5982IHC\u548cH\uff06E\u67d3\u8272\u4e4b\u7c7b\u7684\u65b9\u6cd5\u6355\u83b7\u4e86\u8fd9\u4e9b\u7a7a\u95f4\u7ec6\u8282\uff0c\u4f46\u662f\u5355\u4e2a\u5b9e\u9a8c\u53ea\u80fd\u63ed\u793a\u6709\u9650\u7684\u4fe1\u606f\uff0c\u65e0\u6cd5\u5f97\u5230\u6574\u4e2a\u5fae\u73af\u5883\u7684\u5168\u5c40\u89c6\u56fe\u3002Bendall\u6307\u51fa\uff0c\u4ed6\u66fe\u770b\u5230\u8fc7\u4ed6\u4eec\u90e8\u95e8\u7684\u75c5\u7406\u5b66\u5bb6\u628a\u5e7b\u706f\u7247\u653e\u5728\u4e00\u8d77\uff0c\u4e00\u5f20\u5f20\u770b\uff0c\u4ece\u800c\u89c2\u5bdf\u591a\u4e2a\u7279\u5f81\u30022014\u5e74\u5f00\u53d1\u7684\u4e24\u79cd\u6210\u50cf\u6280\u672f\u53ef\u4ee5\u63d0\u4f9b\u4e30\u5bcc\u7684\u7a7a\u95f4\u6570\u636e\uff0c\u800c\u65e0\u9700\u628a\u591a\u5f20\u56fe\u7247\u5806\u5728\u4e00\u8d77\u6574\u7406\u6570\u636e\u3002<\/span><\/p>\n

\u7b2c\u4e00\u79cd\u6210\u50cf\u6280\u672f\u662f\u8d28\u8c31\u6d41\u5f0f\u7ec6\u80de\u6210\u50cf\u672f\uff08imaging mass cytometry, IMC\uff09\uff0c\u7531\u745e\u58eb\u82cf\u9ece\u4e16\u5927\u5b66\uff08University of Zurich\uff09\u7684Bernd Bodenmiller\u7b49\u4eba\u5f00\u53d1\u3002\u4e0e\u5e38\u89c4CyTOF\u4e00\u6837\uff0cIMC\u6837\u54c1\u91c7\u7528\u91d1\u5c5e\u540c\u4f4d\u7d20\u6807\u8bb0\u7684\u6297\u4f53\uff0c\u4f46\u5728Bodenmiller\u7684\u6280\u672f\u4e2d\uff0c\u6297\u4f53\u6807\u8bb0\u5728\u5b8c\u6574\u7ec4\u7ec7\u4e0a\uff0c\u800c\u4e0d\u662f\u89e3\u79bb\u7684\u7ec6\u80de\u4e0a\u3002\u7136\u540e\u7528\u6fc0\u5149\u626b\u63cf\u6837\u672c\uff0c\u7834\u574f\u7ec4\u7ec7\u5e76\u91ca\u653e\u540c\u4f4d\u7d20\u6807\u8bb0\uff0c\u518d\u7528CyTOF\u5206\u6790\u3002\u8fd9\u4f7f\u5f97\u7814\u7a76\u4eba\u5458\u80fd\u591f\u5728\u5b8c\u6574\u6837\u54c1\u4e0a\u4ee5\u4e9a\u7ec6\u80de\u5206\u8fa8\u7387\u540c\u65f6\u6d4b\u91cf\u6570\u5341\u79cd\u6807\u8bb0\u7269\uff0c\u5176\u7ec6\u8282\u6c34\u5e73\u5927\u5927\u8d85\u8fc7IHC\u3002Bodenmiller\u8868\u793a\uff0cIMC\u80fd\u5206\u6790\u7684\u7ec6\u80de\/\u5206\u5b50\u6570\u91cf\u6bd4IHC\u591a\u51e0\u4e2a\u6570\u91cf\u7ea7\uff0c\u8fd9\u4e3a\u514d\u75ab\u6807\u8bb0\u7269\u7684\u5206\u6790\u5e26\u6765\u4e86\u66f4\u591a\u7684\u5b9a\u91cf\u4fe1\u606f\u3002<\/span><\/p>\n

\u7b2c\u4e8c\u79cd\u6280\u672f\u662fAngelo\u548cBendall\u5728\u65af\u5766\u798f\u5927\u5b66\uff08Stanford University\uff09\u7684\u514d\u75ab\u5b66\u5bb6Garry Nolan\u7684\u5b9e\u9a8c\u5ba4\u5de5\u4f5c\u65f6\u5f00\u53d1\u7684MIBI\u3002MIBI\u540c\u6837\u4f7f\u7528\u540c\u4f4d\u7d20\u6807\u8bb0\u7684\u6297\u4f53\u6807\u8bb0\u6837\u672c\uff0c\u7136\u540e\u7528\u626b\u63cf\u79bb\u5b50\u675f\u4ece\u6837\u672c\u4e2d\u91ca\u653e\u51fa\u8fd9\u4e9b\u6807\u8bb0\uff0c\u4f46MIBI\u4f7f\u7528\u4e0d\u540c\u7c7b\u578b\u7684\u8d28\u8c31\u4eea\u8fdb\u884c\u540c\u4f4d\u7d20\u5206\u6790\u3002Angelo\u548cBendall\u7ee7\u7eed\u5b8c\u5584\u8fd9\u4e00\u5e73\u53f0\uff0c\u4ee5\u63d0\u9ad8\u5176\u901f\u5ea6\u548c\u6613\u7528\u6027\uff0c\u6b63\u5982\u4ed6\u4eec\u57282018\u5e74\u9488\u5bf9\u4e09\u9634\u6027\u4e73\u817a\u764c\u7684\u7814\u7a76\u6240\u5f3a\u8c03\u7684\u90a3\u6837\u3002Bendall\u6307\u51fa\uff0c\u8fc7\u53bb\u5e38\u5e38\u9700\u8981\u82b1\u8d39\u8fd1\u4e00\u5929\u6765\u83b7\u53d6\u4e00\u5f20\u5c0f\u56fe\u7247\uff0c\u4f46\u4ed6\u4eec\u6700\u7ec8\u5728\u5927\u7ea6\u4e00\u5468\u534a\u5185\u5bf9\u5927\u7ea640\u540d\u60a3\u8005\u7684\u6837\u672c\u8fdb\u884c\u4e86\u5206\u6790\uff0c\u5e76\u5206\u522b\u83b7\u53d6\u4e86\u6beb\u7c73\u5927\u5c0f\u7684\u65b9\u5f62\u56fe\u50cf\u3002<\/span><\/p>\n

\u867d\u7136\u8fd9\u4e24\u79cd\u6280\u672f\u4ec5\u7528\u4e8e\u5c11\u6570\u5df2\u53d1\u8868\u7684\u7814\u7a76\uff0c\u4f46MIBI\u548cIMC\u5df2\u7ecf\u8bc1\u660e\u4e86\u83b7\u53d6\u7ec6\u80de\u5728\u80bf\u7624\u5fae\u73af\u5883\u4e2d\u7684\u7a7a\u95f4\u4f4d\u7f6e\u76f8\u5173\u4fe1\u606f\u7684\u4ef7\u503c\u3002Bodenmiller\u6307\u51fa\uff0c\u5927\u5bb6\u90fd\u8bf4\u80bf\u7624\u662f\u9ad8\u5ea6\u5f02\u8d28\u548c\u968f\u673a\u7684\u7cfb\u7edf\uff0c\u4f46\u4ed6\u4eec\u53d1\u73b0\u5f88\u591a\u7ed3\u6784\u548c\u7ec6\u80de\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u5e76\u4e0d\u662f\u968f\u673a\u7684\u3002<\/span><\/p>\n

Merad\u8b66\u544a\u79f0\uff0cIMC\u548cMIBI\u73b0\u5df2\u4e0a\u5e02\uff0c\u4f46\u5b66\u4e60\u8fd9\u4e24\u79cd\u6280\u672f\u5e76\u4e0d\u7b80\u5355\u3002\u8fd9\u4e9b\u57fa\u4e8e\u8d28\u8c31\u7684\u7cfb\u7edf\u5bb9\u6613\u53d7\u5230\u5f88\u591a\u56e0\u7d20\u7684\u5e72\u6270\uff0c\u5979\u8ba4\u4e3a\u73b0\u573a\u9700\u8981\u4e00\u540d\u5de5\u7a0b\u5e08\u6765\u4fdd\u62a4\u8bbe\u5907\u514d\u53d7\u79fb\u52a8\u548c\u5149\u7ebf\u7b49\u73af\u5883\u56e0\u7d20\u7684\u5f71\u54cd\u3002\u867d\u7136\u53ef\u4ee5\u83b7\u5f97\u8d8a\u6765\u8d8a\u591a\u7684\u540c\u4f4d\u7d20\u6807\u8bb0\u6297\u4f53\uff0c\u4f46\u5f00\u53d1\u548c\u4f18\u5316\u65b0\u63a2\u9488\u53ef\u80fd\u662f\u4e00\u4e2a\u8270\u82e6\u7684\u8fc7\u7a0b\u3002Bodenmiller\u4e5f\u6307\u51fa\uff0c\u8fd9\u4e9b\u57fa\u4e8e\u8d28\u8c31\u7684\u65b9\u6cd5\u53ef\u80fd\u4e0d\u5982IHC\u7684\u8367\u5149\u53d8\u4f53\u7075\u654f\uff0c\u540e\u8005\u901a\u5e38\u4f7f\u7528\u4fe1\u53f7\u653e\u5927\u7b56\u7565\uff0c\u5373\u4f7f\u662f\u975e\u5e38\u7a00\u7f3a\u7684\u86cb\u767d\u8d28\u4e5f\u80fd\u88ab\u53ef\u89c6\u5316\u3002<\/span><\/p>\n

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\u65b0\u578b\u8bca\u65ad\u65b9\u6cd5<\/strong>
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\u8fd9\u4e9b\u65b9\u6cd5\u7684\u4e34\u5e8a\u6548\u7528\u5df2\u5f97\u5230\u4e86\u521d\u6b65\u8bc1\u5b9e\u30022017\u5e74Bodenmiller\u548cPe’er\u4e4b\u95f4\u7684\u5408\u4f5c\u8868\u660e\uff0c\u67d0\u4e9b\u514d\u75ab\u8c31\u53ef\u4ee5\u4e3a\u60a3\u6709\u67d0\u79cd\u5f62\u5f0f\u7684\u80be\u764c\u60a3\u8005\u63d0\u4f9b\u9884\u540e\u3002Bodenmiller\u7b49\u4eba\u57fa\u4e8e\u6570\u636e\uff0c\u5f97\u51fa\u4e86\u4e00\u4e2a\u65b9\u7a0b\u5f0f\uff0c\u4ed6\u4eec\u53ef\u4ee5\u7528\u5b83\u6765\u8ba1\u7b97\u8fd9\u4e9b\u60a3\u8005\u7684\u65e0\u8fdb\u5c55\u751f\u5b58\u671f\u3002\u540c\u6837\uff0cMerad\u548cRegev\u5229\u7528\u80bf\u7624\u5fae\u73af\u5883\u8c03\u67e5\u7684\u89c1\u89e3\u6765\u786e\u5b9a\u53ef\u80fd\u514b\u670d\u80ba\u764c\u3001\u4e73\u817a\u764c\u548c\u5176\u5b83\u764c\u75c7\u4e2d\u7684\u8010\u836f\u6027\u548c\u514d\u75ab\u6291\u5236\u95ee\u9898\u7684\u6cbb\u7597\u7597\u7a0b\uff1b\u76ee\u524d\u8fd9\u4e9b\u6cbb\u7597\u8bfe\u7a0b\u7684\u4e34\u5e8a\u8bd5\u9a8c\u6b63\u5728\u51c6\u5907\u4e2d\u3002<\/span><\/p>\n

\u7136\u800c\uff0c\u6784\u5efa\u7528\u4e8e\u5168\u9762\u5fae\u73af\u5883\u5206\u6790\u7684\u5b9e\u9a8c\u548c\u5206\u6790\u7ba1\u9053\u53ef\u80fd\u7ed9\u4e34\u5e8a\u8bbe\u65bd\u5e26\u6765\u6c89\u91cd\u8d1f\u62c5\u3002Merad\u7684\u90e8\u95e8\u7ec4\u5efa\u4e86\u4e00\u4e2a\u7531\u5916\u79d1\u533b\u751f\u3001\u75c5\u7406\u5b66\u5bb6\u3001\u6280\u672f\u4e13\u5bb6\u548c\u764c\u75c7\u751f\u7269\u5b66\u5bb6\u7ec4\u6210\u7684\u7d27\u5bc6\u96c6\u6210\u7684\u56e2\u961f\uff0c\u4ee5\u53ca\u6570\u767e\u4e07\u7f8e\u5143\u7684\u5c16\u7aef\u5668\u68b0\u5e93\uff0c\u5176\u4e2d\u5305\u62ec\u4e09\u53f0CyTOF\u673a\u5668\u3001\u4e09\u53f0\u5355\u7ec6\u80deRNA-seq\u4eea\u5668\u548c\u4e00\u4e2aMIBI\u5e73\u53f0\u3002\u5979\u8868\u793a\uff0c\u8fd9\u9879\u6280\u672f\u975e\u5e38\u6602\u8d35\uff0c\u4e14\u5c5e\u4e8e\u52b3\u52a8\u5bc6\u96c6\u578b\uff0c\u800c\u4e14\u4ed6\u4eec\u8fd8\u6709\u8bb8\u591a\u4e1c\u897f\u4ecd\u5904\u4f18\u5316\u9636\u6bb5\u3002\u5979\u4ece\u672a\u5982\u6b64\u5174\u594b\uff0c\u4f46\u4e5f\u4ece\u6765\u6ca1\u6709\u90a3\u4e48\u7d2f\u3002<\/span><\/p>\n

\u5bf9\u4e8e\u8bb8\u591a\u764c\u75c7\u4e2d\u5fc3\u6765\u8bf4\uff0c\u8fd9\u6837\u7684\u6295\u8d44\u662f\u9065\u4e0d\u53ef\u53ca\u7684\uff0c\u4f46\u8fd9\u4e9b\u5f00\u521b\u6027\u8bbe\u65bd\u7684\u7814\u7a76\u7ed3\u679c\u5f88\u5feb\u5c31\u4f1a\u6e17\u900f\u5230\u66f4\u5e7f\u6cdb\u7684\u793e\u533a\uff0c\u63ed\u793a\u80fd\u7528\u8f83\u4fbf\u5b9c\u7684\u6280\u672f\u8fdb\u884c\u68c0\u6d4b\u7684\u751f\u7269\u6807\u5fd7\u7269\u548c\u75be\u75c5\u6982\u51b5\u3002\u8bf8\u5982\u4eba\u7c7b\u7ec6\u80de\u56fe\u8c31\uff08Human  Atlas\uff09\u4e4b\u7c7b\u7684\u56fd\u9645\u5408\u4f5c\u9879\u76ee\u6b63\u5728\u6574\u5408\u53ef\u516c\u5f00\u83b7\u53d6\u7684\u201c\u6307\u5357\u201d\uff0c\u4ee5\u6307\u5bfc\u57fa\u4e8e\u57fa\u56e0\u7ec4\u5b66\u3001\u8f6c\u5f55\u7ec4\u5b66\u548c\u86cb\u767d\u8d28\u7ec4\u5b66\u7684\u6570\u636e\u5bf9\u7ec6\u80de\uff0c\u5c24\u5176\u662f\u80bf\u7624\u5fae\u73af\u5883\u4e2d\u7684\u7ec6\u80de\u6765\u8fdb\u884c\u5206\u7c7b\u3002\u4f5c\u4e3a\u201c\u6cdb\u764c\u75c7\u56fe\u8c31\u201d\uff08Pan-Cancer Atlas\uff09\u8ba1\u5212\u7684\u4e00\u90e8\u5206\uff0c\u5305\u62ec\u534e\u76db\u987f\u5dde\u897f\u96c5\u56fe\u7cfb\u7edf\u751f\u7269\u5b66\u7814\u7a76\u6240\uff08Institute for Systems Biology in Seattle\uff09\u7684\u8ba1\u7b97\u751f\u7269\u5b66\u5bb6Ilya Shmulevich\u5728\u5185\u7684\u7814\u7a76\u4eba\u5458\u5df2\u7ecf\u5206\u6790\u4e86\u8d85\u8fc71\u4e07\u4e2a\u80bf\u7624\u6807\u672c\u7684\u514d\u75ab\u548c\u80bf\u7624\u7ec6\u80de\u7ec4\u6210\u3002Shmulevich\u6307\u51fa\uff0c\u8fd9\u4e9b\u6570\u636e\u5df2\u7ecf\u5b58\u653e\u5728\u764c\u75c7\u7814\u7a76\u6240\uff08Cancer Research Institute\uff09\u7684iAtlas\u5e93\uff08\u4e00\u4e2a\u514d\u8d39\u63d0\u4f9b\u7684\u6570\u5b57\u8d44\u6e90\uff0c\u4e34\u5e8a\u7814\u7a76\u4eba\u5458\u53ef\u80fd\u6709\u4e00\u5929\u4f1a\u7528\u5b83\u6765\u5bf9\u81ea\u5df1\u7684\u6837\u672c\u8fdb\u884c\u5206\u7c7b\uff09\u4e2d\u3002<\/span><\/p>\n

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Pe’er\u73b0\u5728\u5e0c\u671b\u6280\u672f\u53d1\u5c55\u7684\u811a\u6b65\u80fd\u653e\u7f13\uff0c\u4ece\u800c\u8ba9\u66f4\u591a\u7684\u79d1\u5b66\u5bb6\u80fd\u8fce\u5934\u8d76\u4e0a\u3002\u5979\u8868\u793a\uff0c\u5979\u4eec\u521a\u5f00\u53d1\u4e86\u4e00\u5806\u65b0\u5de5\u5177\uff0c\u73b0\u5728\u5979\u4eec\u9700\u8981\u5f00\u53d1\u66f4\u591a\u7684\u8ba1\u7b97\u65b9\u6cd5\uff0c\u4ece\u66f4\u591a\u7684\u961f\u5217\u4e2d\u6536\u96c6\u66f4\u591a\u7684\u60a3\u8005\u6570\u636e\uff0c\u4ee5\u4e86\u89e3\u8fd9\u4e9b\u5de5\u5177\u80fd\u544a\u8bc9\u4eba\u4eec\u54ea\u4e9b\u4fe1\u606f\u3002<\/span><\/p>\n

\u539f\u6587\u68c0\u7d22\uff1a
Michael Eisenstein. (2019) ular censuses to guide cancer care. Nature<\/em>, 567: 555-557.
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\u70b9\u51fb\u9605\u8bfb\uff0c\u5373\u53ef\u62a5\u540d\u53c2\u52a0\uff01<\/strong><\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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\u8bf7\u6ce8\u660e\uff1a\u59d3\u540d+\u7814\u7a76\u65b9\u5411\uff01<\/strong><\/span><\/p>\n

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