{"id":90437,"date":"2019-04-22T00:00:50","date_gmt":"2019-04-23T04:18:45","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/90437\/"},"modified":"2019-04-23T12:18:49","modified_gmt":"2019-04-23T04:18:49","slug":"a9337be076","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/90437\/","title":{"rendered":"\u5fb7\u5dde\u5927\u5b66\u5723\u5b89\u4e1c\u5c3c\u5965\u533b\u5b66\u4e2d\u5fc3\u9879\u7130\u56e2\u961f\u63ed\u793a\u6291\u5236\u75d8\u75c5\u6bd2\u611f\u67d3\u5bbf\u4e3b\u4f5c\u7528\u673a\u5236"},"content":{"rendered":"
\n

\"\u5fb7\u5dde\u5927\u5b66\u5723\u5b89\u4e1c\u5c3c\u5965\u533b\u5b66\u4e2d\u5fc3\u9879\u7130\u56e2\u961f\u63ed\u793a\u6291\u5236\u75d8\u75c5\u6bd2\u611f\u67d3\u5bbf\u4e3b\u4f5c\u7528\u673a\u5236\"<\/p>\n

50\u5e74\u524d\u6709\u5b66\u8005\u62a5\u9053VACV\u4e0d\u80fd\u611f\u67d3CHO\u7ec6\u80de\uff0c 40\u5e74\u524d\u53d1\u73b0CP77\u53ef\u4ee5\u5e2e\u52a9VACV\u5728CHO\u7ec6\u80de\u4e2d\u590d\u5236\u3002<\/span><\/span>\u8fd1\u6765\uff0c\u5fb7\u5dde\u5927\u5b66\u5723\u5b89\u4e1c\u5c3c\u5965\u533b\u5b66\u4e2d\u5fc3\u7684\u9879\u7130\u5b9e\u9a8c\u5ba4\u7814\u7a76\u7ed3\u679c\u63ed\u793a\u4e86\u5728\u8fd9\u80cc\u540e\u8d77\u91cd\u8981\u4f5c\u7528\u7684\u7ec6\u80de\u56e0\u5b50\u53ca\u673a\u5236\u3002<\/span>\u76f8\u5173\u6210\u679c\u53d1\u8868\u5728\u8001\u724c\u56fd\u9645\u75c5\u6bd2\u5b66\u6743\u5a01\u671f\u520a<\/span><\/span><\/strong><\/span><\/p>\n

Journal of Virology<\/span><\/em><\/strong>\uff0c\u9898\u4e3a<\/span>Identification of CP77 as the third orthopoxvirus SAMD9L inhibitor with a unique specificity for a rodent SAMD9L<\/span><\/strong><\/em>\u3002<\/h3>\n

<\/span><\/strong><\/p>\n

\"\u5fb7\u5dde\u5927\u5b66\u5723\u5b89\u4e1c\u5c3c\u5965\u533b\u5b66\u4e2d\u5fc3\u9879\u7130\u56e2\u961f\u63ed\u793a\u6291\u5236\u75d8\u75c5\u6bd2\u611f\u67d3\u5bbf\u4e3b\u4f5c\u7528\u673a\u5236\"<\/p>\n


<\/span><\/p>\n

\u6b63\u75d8\u75c5\u6bd2\u5c5e <\/span><\/strong>\uff08Orthopoxviruses OPXVs\uff09\u6210\u5458\u5728\u54fa\u4e73\u52a8\u7269\u7ec6\u80de\u7ec6\u80de\u4e2d\u62e5\u6709\u975e\u5e38\u5e7f\u6cdb\u7684\u5bbf\u4e3b\u8303\u56f4\uff0c\u4f46\u662f\u75d8\u82d7\u75c5\u6bd2\uff08vaccinia virus\uff0cVACV)\u5374\u65e0\u6cd5\u611f\u67d3\u4e2d\u56fd\u4ed3\u9f20\u5375\u5de2\u7ec6\u80de (CHO)\u3002\u6211\u4eec\u53d1\u73b0\u5177\u6709\u79cd\u5c5e\u7279\u5f02\u6027\u7684\u5bbf\u4e3b\u6291\u5236\u56e0\u5b50SAMD9L\u4f5c\u4e3a\u4e3b\u8981\u7684\u5bbf\u4e3b\u6291\u5236\u56e0\u5b50\u6291\u5236\u4e86\u75d8\u82d7\u75c5\u6bd2\u7684\u590d\u5236\uff0c\u540c\u65f6\uff0c\u6b63\u75d8\u75c5\u6bd2\u5c5e\u7684CP77\u86cb\u767d\u4f5c\u4e3a\u552f\u4e00\u7684\u62ee\u6297\u56e0\u5b50\u53ef\u4ee5\u62ee\u6297\u4e2d\u56fd\u4ed3\u9f20SAMD9L\u7684\u529f\u80fd\u3002\u5df2\u7ecf\u62a5\u9053\u8fc7\u7684\u7531VACV\u7f16\u7801\u7684 SAMD9 \u548c SAMD9L (SAMD9&L) \u62ee\u6297\u56e0\u5b50 K1 \u548c C7 \u80fd\u591f\u7ed3\u5408\u4eba\u548c\u5c0f\u9f20\u7684 SAMD9&L, \u4f46\u662f\u5747\u4e0d\u80fd\u7ed3\u5408\u4e2d\u56fd\u4ed3\u9f20SAMD9L (chSAMD9L). \u901a\u8fc7CRISPR-Cas9 \u6280\u672f\u6572\u9664CHO\u7ec6\u80de\u4e2d\u7684 chSAMD9L \uff0cVACV\u5373\u53ef\u6210\u529f\u590d\u5236\u3002\u5728VACV\u80fd\u591f\u6210\u529f\u590d\u5236\u7684\u4eba\u6e90\u7ec6\u80de\u4e2d\u8868\u8fbechSAMD9L\u540e\uff0c VACV\u4fbf\u65e0\u6cd5\u987a\u5229\u5b8c\u6210\u590d\u5236\u3002\u4e0e K1 \u548c C7\u8fd9\u4e00\u5df2\u7ecf\u62a5\u9053\u8fc7\u7684SAMD9&L\u62ee\u6297\u56e0\u5b50\u4e0d\u540c, \u725b\u75d8\u75c5\u6bd2\u7f16\u7801\u7684CP77\u86cb\u767d\u53ef\u4ee5\u7ed3\u5408chSAMD9L \u5e76\u4e14\u53ef\u4ee5\u4f7fVACV \u5728\u8868\u8fbe chSAMD9L \u7684\u7ec6\u80de\u4e2d\u6210\u529f\u590d\u5236, \u8868\u660eCP77\u662f SAMD9L \u7684\u53e6\u4e00\u4e2a\u7279\u5f02\u6027\u62ee\u6297\u56e0\u5b50\u3002\u800c\u4e14CP77 \u4e0eK1\u7ed3\u5408\u5728SAMD9L \u7684\u540c\u4e00\u4e2a\u7ed3\u6784\u57df\u3002\u75c5\u6bd2\u751f\u957f\u5b9e\u9a8c\u7ed3\u679c\u663e\u793a OPXV \u7684\u6240\u6709\u6210\u5458\u62ee\u6297 chSAMD9L \u7684\u80fd\u529b\u4e0e\u5404\u75c5\u6bd2\u6240\u7f16\u7801 CP77 \u57fa\u56e0\u7684\u72b6\u6001\u6709\u5173\u3002\u5305\u62ec\u7334\u75d8\u75c5\u6bd2\u5728\u5185\u7684\u5f88\u591a OPXVs \u6210\u5458\u5747\u4fdd\u7559\u7740\u529f\u80fd\u6027CP77 \u540c\u6e90\u3002\u8be5\u7814\u7a76\u7ed3\u679c\u63ed\u793a\u4e86\u556e\u9f7f\u7c7b\u52a8\u7269\u4e2dSAMD9L \u7684\u79cd\u5c5e\u7279\u5f02\u6027\u6784\u6210\u4e86\u4e00\u9053\u6291\u5236OPXV \u8de8\u79cd\u95f4\u4f20\u64ad\u7684\u5929\u7136\u5c4f\u969c\u3002\u7136\u800c\uff0cOPXVs \u8fdb\u5316\u51fa\u4e863\u4e2a\u5177\u6709\u4e0d\u540c\u7279\u5f02\u6027\u7684SAMD9L \u62ee\u6297\u56e0\u5b50\u6765\u8de8\u8d8a\u8fd9\u9053\u5c4f\u969c\u3002<\/span>
<\/p>\n


<\/p>\n

\u5305\u62ec\u7334\u75d8\u75c5\u6bd2\u548c\u725b\u75d8\u75c5\u6bd2\u5728\u5185\u7684\u591a\u4e2a\u6b63\u75d8\u75c5\u6bd2\u5c5e\u6210\u5458\u53ef\u4ee5\u5f15\u8d77\u52a8\u7269\u75ab\u6e90\u6027\u75be\u75c5\u3002\u6709\u8db3\u591f\u7684\u8bc1\u636e\u663e\u793a\u8fd9\u4e9b\u75c5\u6bd2\u5229\u7528\u91ce\u751f\u7684\u556e\u9f7f\u7c7b\u52a8\u7269\u4f5c\u4e3a\u50a8\u5b58\u5bbf\u4e3b\u548c\u4e2d\u95f4\u5bbf\u4e3b\uff0c\u4f46\u662f\uff0c\u6211\u4eec\u5bf9\u4e8e\u75c5\u6bd2\u4e0e\u5bbf\u4e3b\u76f8\u4e92\u4f5c\u7528\u5982\u4f55\u51b3\u5b9a\u4e86\u6b63\u75d8\u75c5\u6bd2\u5c5e\u6210\u5458\u5728\u556e\u9f7f\u7c7b\u52a8\u7269\u4e2d\u7684\u4f20\u64ad\u8fd8\u4e0d\u751a\u4e86\u89e3\u3002\u6b63\u75d8\u75c5\u6bd2\u5c5e\u90e8\u5206\u6210\u5458\u65e0\u6cd5\u5728CHO\u7ec6\u80de\u4e2d\u8fdb\u884c\u590d\u5236\u662f\u7531\u4e8e\u5bbf\u4e3b\u6297\u75c5\u6bd2\u56e0\u5b50SAMD9L \u7684\u6291\u5236\u4f5c\u7528\u5bfc\u81f4\u7684\uff0c\u4e0d\u540c\u556e\u9f7f\u7c7b\u52a8\u7269\u4e2dSAMD9L \u7684\u5dee\u522b\u6784\u6210\u4e86\u4e00\u9053\u6291\u5236\u90e8\u5206\u6b63\u75d8\u75c5\u6bd2\u8de8\u79cd\u5c5e\u4f20\u64ad\u7684\u5c4f\u969c\u3002\u6b63\u75d8\u75c5\u6bd2\u5c5e\u7684K1\u548cC7\u80fd\u591f\u62ee\u6297\u4eba\u7c7b\u548c\u5c0f\u9f20\u7684 SAMD9L\uff0c\u4f46\u662f\u65e0\u6cd5\u62ee\u6297\u4e2d\u56fd\u4ed3\u9f20\u7684 SAMD9L\u3002\u4e2d\u56fd\u4ed3\u9f20\u7684 SAMD9L\u53ea\u80fd\u591f\u88ab\u5177\u6709\u5b8c\u6574\u529f\u80fd\u7684CP77\u86cb\u767d\u6240\u62ee\u6297\u3002\u8868\u660e\u6b63\u75d8\u75c5\u6bd2\u5c5e\u6210\u5458\u7f16\u7801\u4e09\u79cd\u5177\u6709\u4e0d\u540c\u7279\u5f02\u6027\u7684SAMD9L \u62ee\u6297\u56e0\u5b50\u3002\u6211\u4eec\u7684\u7814\u7a76\u7ed3\u679c\u8868\u660e\u6b63\u75d8\u75c5\u6bd2\u5728\u556e\u9f7f\u52a8\u7269\u4e2d\u7684\u5bbf\u4e3b\u8303\u56f4 \u53d6\u51b3\u4e8e\u4e09\u79cdSAMD9L \u62ee\u6297\u56e0\u5b50\uff08K1, C7, CP77\uff09\u7684\u4e0d\u540c\u7279\u5f02\u6027\u3002<\/span><\/p>\n


<\/p>\n

\u7814\u7a76\u7ed3\u679c\uff1a<\/span><\/strong><\/span><\/p>\n

 <\/span><\/p>\n

1.   \u4e2d\u56fd\u4ed3\u9f20 SAMD9 \u548c SAMD9L \u4e0e\u75d8\u75c5\u6bd2\u86cb\u767d\u7684\u7ed3\u5408\u5177\u6709\u7279\u5f02\u6027.<\/span><\/p>\n

\"\u5fb7\u5dde\u5927\u5b66\u5723\u5b89\u4e1c\u5c3c\u5965\u533b\u5b66\u4e2d\u5fc3\u9879\u7130\u56e2\u961f\u63ed\u793a\u6291\u5236\u75d8\u75c5\u6bd2\u611f\u67d3\u5bbf\u4e3b\u4f5c\u7528\u673a\u5236\"<\/p>\n

2.  chSAMD9L \u662f\u6291\u5236 VACV \u5728CHO \u7ec6\u80de\u4e2d\u7684\u5fc5\u8981\u6761\u4ef6; CP77 \u662f SAMD9L \u7684\u7279\u5f02\u6027\u62ee\u6297\u56e0\u5b50.<\/span><\/p>\n

\"\u5fb7\u5dde\u5927\u5b66\u5723\u5b89\u4e1c\u5c3c\u5965\u533b\u5b66\u4e2d\u5fc3\u9879\u7130\u56e2\u961f\u63ed\u793a\u6291\u5236\u75d8\u75c5\u6bd2\u611f\u67d3\u5bbf\u4e3b\u4f5c\u7528\u673a\u5236\"<\/p>\n

3. \u5728\u4eba\u6e90\u7ec6\u80de\u4e2d\u8868\u8fbe chSAMD9L \u53ef\u4ee5\u6291\u5236\u75d8\u75c5\u6bd2\u7684\u590d\u5236<\/span><\/p>\n

 <\/span><\/p>\n

\"\u5fb7\u5dde\u5927\u5b66\u5723\u5b89\u4e1c\u5c3c\u5965\u533b\u5b66\u4e2d\u5fc3\u9879\u7130\u56e2\u961f\u63ed\u793a\u6291\u5236\u75d8\u75c5\u6bd2\u611f\u67d3\u5bbf\u4e3b\u4f5c\u7528\u673a\u5236\"<\/p>\n

4. CP77\u7ed3\u5408\u4f4d\u70b9\u4f4d\u4e8eSAMD9&L\u7684NTPase-TPR \u7ed3\u6784\u57df.<\/span><\/p>\n

\"\u5fb7\u5dde\u5927\u5b66\u5723\u5b89\u4e1c\u5c3c\u5965\u533b\u5b66\u4e2d\u5fc3\u9879\u7130\u56e2\u961f\u63ed\u793a\u6291\u5236\u75d8\u75c5\u6bd2\u611f\u67d3\u5bbf\u4e3b\u4f5c\u7528\u673a\u5236\"<\/p>\n

5. CP77\u86cb\u767d\u4f4d\u4e8eN\u672b\u7aef\u7684382\u6c28\u57fa\u9178\u5bf9\u4e8e\u7ed3\u5408 chSAMD9L\u8d77\u7740\u91cd\u8981\u4f5c\u7528.<\/span><\/p>\n

\"\u5fb7\u5dde\u5927\u5b66\u5723\u5b89\u4e1c\u5c3c\u5965\u533b\u5b66\u4e2d\u5fc3\u9879\u7130\u56e2\u961f\u63ed\u793a\u6291\u5236\u75d8\u75c5\u6bd2\u611f\u67d3\u5bbf\u4e3b\u4f5c\u7528\u673a\u5236\"<\/p>\n

6. \u6b63\u75d8\u75c5\u6bd2\u79d1\u6210\u5458\u62ee\u6297chSAMD9L \u529f\u80fd\u7684\u80fd\u529b\u4e0e\u5176\u8868\u8fbeCP77\u57fa\u56e0\u7684\u72b6\u6001\u76f8\u5173 <\/span><\/p>\n

Table 1. OPXV CP77 orthologs comparison to CPXV BR CP77.<\/span><\/p>\n

 <\/span><\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\n

OPXV species<\/span><\/p>\n<\/td>\n

\n

CP77<\/span><\/p>\n<\/td>\n

\n

Antagonize chSAMD9L<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

length (aa)<\/span><\/p>\n<\/td>\n

\n

identity (%)<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

CPXV<\/span><\/p>\n<\/td>\n

\n

661-674<\/span><\/p>\n<\/td>\n

\n

91-100<\/span><\/p>\n<\/td>\n

\n

Yes<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

MPXV<\/span><\/p>\n<\/td>\n

\n

659-660<\/span><\/p>\n<\/td>\n

\n

90-91<\/span><\/p>\n<\/td>\n

\n

Yes<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

VACV<\/span><\/p>\n<\/td>\n

\n

small deletions, early frameshift<\/span><\/p>\n<\/td>\n

\n

No<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

VARV<\/span><\/p>\n<\/td>\n

\n

>600 nt deletion, 355-490<\/span><\/p>\n<\/td>\n

\n

86-88<\/span><\/p>\n<\/td>\n

\n

ND<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

ECTV<\/span><\/p>\n<\/td>\n

\n

504 nt deletion, early frameshift<\/span><\/p>\n<\/td>\n

\n

No<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

CMLV<\/span><\/p>\n<\/td>\n

\n

large deletion (~155 nt remaining)<\/span><\/p>\n<\/td>\n

\n

No<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

TATV  <\/span><\/p>\n<\/td>\n

\n

661<\/span><\/p>\n<\/td>\n

\n

92<\/span><\/p>\n<\/td>\n

\n

Yes<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

AKMV<\/span><\/p>\n<\/td>\n

\n

672<\/span><\/p>\n<\/td>\n

\n

79<\/span><\/p>\n<\/td>\n

\n

Yes<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

SKPV<\/span><\/p>\n<\/td>\n

\n

633<\/span><\/p>\n<\/td>\n

\n

69<\/span><\/p>\n<\/td>\n

\n

Yes<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

VPXV<\/span><\/p>\n<\/td>\n

\n

613<\/span><\/p>\n<\/td>\n

\n

69<\/span><\/p>\n<\/td>\n

\n

Yes<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

RNCV<\/span><\/p>\n<\/td>\n

\n

gene fusion*, 406<\/span><\/p>\n<\/td>\n

\n

66<\/span><\/p>\n<\/td>\n

\n

partial<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

*Strain MD1964-85A. 1-406 of CP77 ortholog fused in frame to the N-terminus of the full-length, 246-aa chemokine binding protein. Total protein length is 655-aa.  <\/span><\/p>\n

 <\/span><\/p>\n

\"\u5fb7\u5dde\u5927\u5b66\u5723\u5b89\u4e1c\u5c3c\u5965\u533b\u5b66\u4e2d\u5fc3\u9879\u7130\u56e2\u961f\u63ed\u793a\u6291\u5236\u75d8\u75c5\u6bd2\u611f\u67d3\u5bbf\u4e3b\u4f5c\u7528\u673a\u5236\"<\/p>\n

\u9879\u7130<\/span><\/strong><\/span>\uff0c\u7f8e\u56fd\u5fb7\u514b\u8428\u65af\u5dde\u7acb\u5927\u5b66\u5723\u5b89\u4e1c\u5c3c\u5965\u533b\u5b66\u4e2d\u5fc3\u5fae\u751f\u7269\u548c\u514d\u75ab\u7cfb\u7ec8\u8eab\u6559\u6388\uff0c\u535a\u58eb\u751f\u5bfc\u5e08\uff0c\u4e3b\u8981\u7814\u7a76\u75c5\u6bd2\u4e0e\u5bbf\u4e3b\u7ec6\u80de\u7684\u76f8\u4e92\u4f5c\u7528\u3002\u5176\u73b0\u4efbJournal of Virology \u6742\u5fd7\u7f16\u59d4\uff0cPLoS One\u6742\u5fd7\u7f16\u8f91\u548cPLoS  Pathogen\u3001Virology\u3001Antiviral Therapy\u3001Virology Journal\u3001Journal of General Virology\u7b49\u6742\u5fd7\u53d7\u8058\u5ba1\u7a3f\u4e13\u5bb6\uff0c\u5e76\u53d7\u9080\u62c5\u4efb\u7f8e\u56fdNIH \u75c5\u6bd2\u7814\u7a76\u7ec4\u59d4\u4f1a\u57fa\u91d1\u8bc4\u5ba1\u4e13\u5bb6\u3002\u76ee\u524d\u5176\u627f\u62c5\u7f8e\u56fdNIH\u7b49\u591a\u9879\u8bfe\u9898\u7684\u7814\u7a76\uff0c\u5e76\u53d1\u8868\u8bba\u658740\u4f59\u7bc7\u3002<\/span><\/p>\n

Abstract<\/span><\/strong><\/span><\/h3>\n


<\/p>\n

Orthopoxviruses (OPXVs) have a broad host range in mammalian cells, but Chinese hamster ovary (CHO) cells are non-permissive for vaccinia virus (VACV). Here, we revealed a species-specific difference in host restriction factor SAMD9L as the cause for the restriction and identified orthopoxvirus CP77 as a unique inhibitor capable of antagonizing Chinese hamster SAMD9L (chSAMD9L). Two known VACV inhibitors of SAMD9 and SAMD9L (SAMD9&L);, K1 and C7, can bind human and mouse SAMD9&L;, but neither can bind chSAMD9L. CRISPR-Cas9 knockout of chSAMD9L from CHO cells removed the restriction for VACV, while ectopic expression of chSAMD9L imposed the restriction for VACV in a human cell line, demonstrating that chSAMD9L is a potent restriction factor for VACV. Contrary to K1 and C7, cowpox virus CP77 can bind chSAMD9L and rescue VACV replication in cells expressing chSAMD9L, indicating that CP77 is yet another SAMD9L inhibitor but has a unique specificity for chSAMD9L. Binding studies showed that the N-terminal 382 amino acids of CP77 were sufficient for binding chSAMD9L and that both K1 and CP77 target a common internal region of SAMD9L. Growth studies with nearly all OPXV species showed that the ability of OPXVs in antagonizing chSAMD9L correlates with CP77 gene status and that a functional CP77ortholog was maintained in many OPXVs, including monkeypox virus. Our data suggest that species-specific difference in rodent SAMD9Lposes a barrier for cross-species OPXV infection and that OPXVs have evolved three SAMD9&L inhibitors with different specificities to overcome this barrier.<\/span><\/p>\n


<\/span><\/p>\n

IMPORTANCE<\/span><\/strong>: Several OPXV species, including monkeypox virus and cowpox virus, cause zoonotic infection in humans. They are believed to use wild rodents as the reservoir or intermediate hosts, but the host or viral factors that are important for OPXV host range in rodents are unknown. Here, we showed that the abortive replication of several OPXV species in a Chinese hamster cell line was caused by a species-specific difference in the host antiviral factor SAMD9L, suggesting that SAMD9L divergence in different rodentspecies poses a barrier for cross-species OPXV infection. While the Chinese hamster SAMD9L could not be inhibited by two previously identified OPXV inhibitors of human and mouse SAMD9&L;, it can be inhibited by cowpox virus CP77, indicating that OPXVs encode three SAMD9&L inhibitors with different specificity. Our data suggest that OPXV host range in broad rodent species depends on three SAMD9&L inhibitors with different specificities.<\/span><\/p>\n

\u672c\u671f\u7f16\u8f91\uff1aTony<\/span><\/p>\n

\"\u5fb7\u5dde\u5927\u5b66\u5723\u5b89\u4e1c\u5c3c\u5965\u533b\u5b66\u4e2d\u5fc3\u9879\u7130\u56e2\u961f\u63ed\u793a\u6291\u5236\u75d8\u75c5\u6bd2\u611f\u67d3\u5bbf\u4e3b\u4f5c\u7528\u673a\u5236\"<\/p>\n

\"\u5fb7\u5dde\u5927\u5b66\u5723\u5b89\u4e1c\u5c3c\u5965\u533b\u5b66\u4e2d\u5fc3\u9879\u7130\u56e2\u961f\u63ed\u793a\u6291\u5236\u75d8\u75c5\u6bd2\u611f\u67d3\u5bbf\u4e3b\u4f5c\u7528\u673a\u5236\"<\/p>\n<\/p><\/div>\n","protected":false},"excerpt":{"rendered":"

50\u5e74\u524d\u6709\u5b66\u8005\u62a5\u9053VACV\u4e0d\u80fd\u611f\u67d3CHO\u7ec6\u80de\uff0c 40\u5e74\u524d\u53d1\u73b0CP77\u53ef\u4ee5\u5e2e\u52a9VACV\u5728CHO\u7ec6\u80de\u4e2d\u590d\u5236\u3002\u8fd1\u6765\uff0c […]<\/p>\n","protected":false},"author":1,"featured_media":90438,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[2],"tags":[],"_links":{"self":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/90437"}],"collection":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/comments?post=90437"}],"version-history":[{"count":1,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/90437\/revisions"}],"predecessor-version":[{"id":90449,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/90437\/revisions\/90449"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media\/90438"}],"wp:attachment":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media?parent=90437"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/categories?post=90437"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/tags?post=90437"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}