{"id":9540,"date":"2018-06-13T01:00:00","date_gmt":"2018-07-27T09:39:36","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/2018\/07\/27\/nature%e5%8f%91%e6%96%87%ef%bc%8c%e8%82%a1%e4%bb%b7%e5%a4%a7%e8%b7%8c%ef%bc%81p53%e7%a9%b6%e7%ab%9f%e5%a6%82%e4%bd%95%e5%bd%b1%e5%93%8dcrispr%e7%9a%84%e6%9c%aa%e6%9d%a5%ef%bc%9f\/"},"modified":"2018-07-27T17:39:39","modified_gmt":"2018-07-27T09:39:39","slug":"nature%e5%8f%91%e6%96%87%ef%bc%8c%e8%82%a1%e4%bb%b7%e5%a4%a7%e8%b7%8c%ef%bc%81p53%e7%a9%b6%e7%ab%9f%e5%a6%82%e4%bd%95%e5%bd%b1%e5%93%8dcrispr%e7%9a%84%e6%9c%aa%e6%9d%a5%ef%bc%9f","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/9540\/","title":{"rendered":"Nature\u53d1\u6587\uff0c\u80a1\u4ef7\u5927\u8dcc\uff01p53\u7a76\u7adf\u5982\u4f55\u5f71\u54cdCRISPR\u7684\u672a\u6765\uff1f"},"content":{"rendered":"
\n

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\"Nature\u53d1\u6587\uff0c\u80a1\u4ef7\u5927\u8dcc\uff01p53\u7a76\u7adf\u5982\u4f55\u5f71\u54cdCRISPR\u7684\u672a\u6765\uff1f\"<\/p>\n


<\/p>\n

Nature Medicine<\/span><\/em>\u5728\u672c\u5468\u4e00\u4e0a\u7ebf\u4e86\u4e24\u7bc7CRISPR\u7684\u76f8\u5173\u6587\u7ae0 [1-2]\uff0c\u8fd9\u4e24\u7bc7\u6587\u7ae0\u4e2d\u7684\u7814\u7a76\u5747\u53d1\u73b0CRISPR-Cas9\u7cfb\u7edf\u80fd\u591f\u8bf1\u5bfc\u7ec6\u80de\u5185p53\u4ecb\u5bfc\u7684DNA\u635f\u4f24\u53cd\u5e94\uff0c\u5f71\u54cdCRISPR\u7684\u57fa\u56e0\u7ec4\u7f16\u8f91\u3002<\/span>
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\u6587\u7ae0\u4e0a\u7ebf\u4e4b\u540eCRISPR\u76f8\u5173\u516c\u53f8\u7684\u80a1\u4ef7\u5927\u8dcc\uff0cCRISPR Therapeutics\u4e00\u5ea6\u4e0b\u8dcc\u63a5\u8fd113%\uff0cEditas Medicine\u4e0b\u8dcc\u63a5\u8fd18%\uff0cIntellia Therapeutics\u4e0b\u8dcc\u63a5\u8fd110%\uff0cSangamo Therapeutics\u4e0b\u8dcc\u63a5\u8fd15%\u3002<\/span><\/p>\n

 <\/span><\/p>\n

\u6295\u8d44\u8005\u7684\u53cd\u5e94\u4e4b\u6240\u4ee5\u5982\u6b64\u5f3a\u70c8\uff0c\u4e3b\u8981\u662f\u7531\u4e8e\u5f88\u591a\u4eba\u62c5\u5fc3\u7ecf\u8fc7CRISPR\u7f16\u8f91\u7684\u7ec6\u80de\u5176\u764c\u53d8\u7684\u98ce\u9669\u4f1a\u63d0\u9ad8\u3002\u4f46\u4e8b\u5b9e\u679c\u771f\u5982\u6b64\u5417\uff1f\u6211\u4eec\u5148\u770b\u4e00\u4e0b\u8fd9\u4e24\u7bc7\u6587\u7ae0\u7684\u7814\u7a76\u5185\u5bb9\u3002<\/span><\/p>\n


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\u6587\u7ae0\u89e3\u8bfb<\/span><\/strong><\/span><\/p>\n

 <\/span><\/p>\n

\u8fd9\u4e24\u7bc7\u6587\u7ae0\u7684\u7814\u7a76\u4eba\u5458\u5206\u522b\u6765\u81ea\u5361\u7f57\u6797\u65af\u5361\u7814\u7a76\u6240 (Karolinska Institute)\u4ee5\u53ca\u8bfa\u534e\u3002\u8bfa\u534e\u548c\u5361\u7f57\u6797\u65af\u5361\u7684\u7814\u7a76\u4eba\u5458\u5206\u522b\u4f7f\u7528\u4e86\u4e0d\u540c\u79cd\u7c7b\u7684\u7ec6\u80de\uff0c\u5361\u7f57\u6797\u65af\u5361\u7684\u5b9e\u9a8c\u5ba4\u4f7f\u7528\u7684\u662f\u4e00\u79cd\u89c6\u7f51\u819c\u8272\u7d20\u4e0a\u76ae\u7ec6\u80de (RPE1)\u3002\u4ed6\u4eec\u5728\u5b9e\u9a8c\u4e2d\u4f7f\u7528\u7684RPE1\u5e76\u4e0d\u5c5e\u4e8e\u8f6c\u5316\u7ec6\u80de\uff0c\u53ea\u662f\u5176\u7aef\u7c92\u9176\u9006\u8f6c\u5f55\u9176\u80fd\u591f\u7a33\u5b9a\u8868\u8fbe\uff0c\u4ece\u800c\u6291\u5236\u7aef\u7c92\u9176\u6d3b\u6027\uff0c\u4f7f\u5176\u6c38\u751f\u5316\u3002\u8be5\u7c7b\u7ec6\u80de\u6765\u6e90\u4e8e\u4eba\u7684\u89c6\u7f51\u819c\u8272\u7d20\u4e0a\u76ae\u7ec6\u80de\uff0c\u4e0e\u6cbb\u7597\u89c6\u7f51\u819c\u9000\u884c\u6027\u75be\u75c5\u7b49CRISPR\u76f8\u5173\u7597\u6cd5\u7684\u7814\u53d1\u5177\u6709\u6bd4\u8f83\u9ad8\u7684\u76f8\u5173\u6027\u3002<\/span><\/p>\n

 <\/span><\/p>\n

\u5361\u7f57\u6797\u65af\u5361\u5b9e\u9a8c\u5ba4\u7684\u53d1\u73b0\u4e5f\u7b97\u662f\u610f\u5916\uff0c\u4ed6\u4eec\u5728\u4f7f\u7528CRISPR\u2013Cas9\u7cfb\u7edf\u5bf9\u591a\u79cd\u7c7b\u578b\u7684\u7ec6\u80de\u7cfb\u8fdb\u884cdropout screens\u5b9e\u9a8c\u7684\u65f6\u5019\u53d1\u73b0\uff0c\u5728RPE1\u7ec6\u80de\u4e2d\u9776\u5411\u91cd\u8981\u57fa\u56e0\u7684guide\u5e8f\u5217\u5e76\u6ca1\u6709\u88ab\u6709\u6548\u7684\u6e05\u9664\u3002<\/span><\/p>\n


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\u4f46\u4ed6\u4eec\u540c\u65f6\u4e5f\u53d1\u73b0\u9776\u5411TP53\u57fa\u56e0(\u80fd\u591f\u7f16\u7801p53)\u7684guide\u6d53\u5ea6\u663e\u8457\u63d0\u5347\u3002\u4f5c\u8005\u8ba4\u4e3a\u8fd9\u79cd\u73b0\u8c61\u53ef\u80fd\u662f\u7531\u4e8eCas9\u5f15\u53d1\u7684\u53cc\u94feDNA\u65ad\u88c2(DSB)\u80fd\u591f\u6d3b\u5316p53\uff0c\u4ece\u800c\u5bfc\u81f4\u7ec6\u80de\u751f\u957f\u6291\u5236\u3002<\/span>
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\u8be5\u5b9e\u9a8c\u5ba4\u4e5f\u901a\u8fc7\u4e00\u7cfb\u5217\u5b9e\u9a8c\u8bc1\u5b9e\u4e86\u4ee5\u4e0a\u63a8\u8bba\uff1a\u5982\u679c\u5728p53\u529f\u80fd\u6b63\u5e38\u7684\u7ec6\u80de\u4e2d\u4f7f\u7528Cas9\u5bf9\u57fa\u56e0\u7ec4\u8fdb\u884c\u7f16\u8f91\u7684\u8bdd\uff0c\u5c31\u80fd\u591f\u5f15\u53d1DNA\u635f\u4f24\u53cd\u5e94\uff0c\u5bfc\u81f4\u7ec6\u80de\u5468\u671f\u505c\u6ede\u3002<\/span><\/p>\n


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\u4f46\u4ece\u6587\u7ae0\u4e2d\u6211\u4eec\u4e5f\u53ef\u4ee5\u770b\u51fa\u8fd9\u4e00\u73b0\u8c61\u4f9d\u8d56DSB\u7684\u5b58\u5728\uff0c\u5982\u679c\u4f7f\u7528\u4e0d\u5177\u5907\u526a\u5207\u529f\u80fd\u7684CRISPR\u7cfb\u7edf\u5bf9\u57fa\u56e0\u7ec4\u8fdb\u884c\u7f16\u8f91\u4e5f\u5c31\u6709\u53ef\u80fd\u4e0d\u4f1a\u4ea7\u751f\u8fd9\u4e00\u73b0\u8c61\u3002<\/span><\/p>\n


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\u8be5\u7814\u7a76\u4e5f\u53d1\u73b0\u5982\u679c\u6291\u5236DNA\u635f\u4f24\u53cd\u5e94\u7684\u8bdd\u5c31\u80fd\u591f\u63d0\u9ad8CRISPR\u5728\u6b63\u5e38\u7684\u975e\u8f6c\u5316\u7ec6\u80de\u4e2d\u7684\u7cbe\u51c6\u7f16\u8f91\u6548\u7387\uff0c\u4f46\u8fd9\u79cd\u64cd\u4f5c\u540c\u65f6\u4e5f\u4f1a\u4f7f\u635f\u4f24\u7684\u57fa\u56e0\u7ec4\u9003\u8fc7\u80de\u5185\u7684DNA\u635f\u4f24\u68c0\u6d4b\u7cfb\u7edf\u3002<\/span><\/p>\n

 <\/span><\/p>\n

\u76f8\u6bd4\u5361\u7f57\u6797\u65af\u5361\u7684\u6587\u7ae0\uff0c\u8bfa\u534e\u7684\u6587\u7ae0<\/span>\u5173\u6ce8\u5ea6\u66f4\u9ad8(\u6216\u8005\u8bf4\u8bdd\u9898\u6027\u66f4\u9ad8\uff1f) \u3002\u8bfa\u534e\u5728\u8be5\u9879\u7814\u7a76\u4e2d\u4f7f\u7528\u7684\u662f\u4eba\u591a\u80fd\u5e72\u7ec6\u80de\u3002\u7531\u4e8e\u8fd9\u7c7b\u7ec6\u80de\u80fd\u591f\u5206\u5316\u5f62\u6210\u51e0\u4e4e\u4efb\u4f55\u7c7b\u578b\u7684\u7ec6\u80de\uff0c\u56e0\u6b64\u5176\u4e5f\u6709\u53ef\u80fd\u88ab\u5e94\u7528\u4e8e\u5f88\u591a\u7c7b\u578b\u75be\u75c5\u7684\u6cbb\u7597\u3002<\/span><\/span><\/p>\n


<\/span><\/p>\n

\u4f46\u4ee5\u524d\u7684\u5f88\u591a\u7814\u7a76\u53d1\u73b0\u76f8\u6bd4\u5176\u4ed6\u7c7b\u578b\u7684\u7ec6\u80de\u800c\u8a00\uff0c\u4eba\u591a\u80fd\u5e72\u7ec6\u80de\u4e2d\u7684\u57fa\u56e0\u9776\u5411\u6548\u7387\u8981\u4f4e\u5f88\u591a\u3002\u56e0\u6b64\u8bfa\u534e\u7684\u56e2\u961f\u5e0c\u671b\u80fd\u591f\u63d0\u9ad8CRISPR\u5728\u591a\u80fd\u5e72\u7ec6\u80de\u4e2d\u7684\u57fa\u56e0\u7f16\u8f91\u6548\u7387\u3002<\/span><\/p>\n


<\/span><\/p>\n

\u8bfa\u534e\u7684\u56e2\u961f\u901a\u8fc7All in one inducible Cas9 + doxycycline\u7684\u4f53\u7cfb\u5c06\u591a\u80fd\u5e72\u7ec6\u80de\u4e2d\u7684\u7f16\u8f91(Indels)\u6548\u7387\u63d0\u9ad8\u5230\u4e8680%\u3002\u4f46\u4ed6\u4eec\u540c\u65f6\u4e5f\u53d1\u73b0\u7ecf\u8fc7\u57fa\u56e0\u7f16\u8f91\u4e4b\u540e\u53ea\u6709\u5f88\u5c0f\u4e00\u90e8\u5206\u7ec6\u80de\u80fd\u591f\u5b58\u6d3b\u3002\u800c\u4e14\u66f4\u52a0\u4e0d\u5e78\u7684\u662f\uff0c\u4ed6\u4eec\u6700\u540e\u53d1\u73b0\u4e4b\u6240\u4ee5CRISPR\u80fd\u591f\u6210\u529f\u7f16\u8f91\u8fd9\u4e9b\u7ec6\u80de\uff0c\u662f\u56e0\u4e3a\u8fd9\u4e9b\u7ec6\u80de\u7684p53\u529f\u80fd\u5b58\u5728\u7f3a\u5931\uff0c\u624d\u4f7f\u5f97\u57fa\u56e0\u7ec4\u7f16\u8f91\u80fd\u591f\u987a\u5229\u8fdb\u884c\u3002<\/span><\/p>\n

 <\/span><\/p>\n

\u5176\u5b9e\u8fd9\u4e5f\u5f88\u5bb9\u6613\u7406\u89e3\uff0c\u56e0\u4e3a\u591a\u80fd\u5e72\u7ec6\u80de\u4e0e\u65e9\u671f\u80da\u80ce\u5185\u7684\u7ec6\u80de\u975e\u5e38\u7c7b\u4f3c\uff0c\u5bf9\u4e8eDSB\u7684\u6781\u7aef\u654f\u611f\u80fd\u591f\u4ece\u67d0\u79cd\u7a0b\u5ea6\u4e0a\u6291\u5236\u80da\u80ce\u4e2d\u5f02\u5e38\u7ec6\u80de\u7684\u8fdb\u4e00\u6b65\u589e\u6b96\uff0c\u8fd9\u4e5f\u662f\u4e00\u79cd\u81ea\u6211\u4fdd\u62a4\u673a\u5236\u3002<\/span><\/p>\n


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\u8fd9\u4e24\u7bc7\u6587\u7ae0\u4e2dp53\u6240\u626e\u6f14\u7684\u529f\u80fd\u6709\u4e00\u4e9b\u4e0d\u540c\uff0c\u5728\u5361\u7f57\u6797\u65af\u5361\u7684\u6587\u7ae0\u4e2d\uff0cDNA\u7684\u53cc\u94fe\u65ad\u88c2\u80fd\u591f\u6d3b\u5316p53\uff0c\u5bfc\u81f4\u7ec6\u80de\u5468\u671f\u505c\u6ede\u3002\u4f46\u5728\u8bfa\u534e\u7684\u6587\u7ae0\u4e2dp53\u7684\u5f71\u54cd\u8981\u4e25\u91cd\u7684\u591a\uff0c<\/span><\/span>\u56e0\u4e3a\u4ed6\u4eec\u53d1\u73b0\u80fd\u591f\u6210\u529f\u8fdb\u884c\u7f16\u8f91\u7684\u7ec6\u80de\u5f88\u591a\u90fd\u662f\u5b58\u5728p53\u529f\u80fd\u7f3a\u9677\u7684\u7ec6\u80de\uff0c\u800c\u4e0d\u5b58\u5728p53\u7f3a\u9677\u7684\u7ec6\u80de\u5374\u6ca1\u6709\u6b63\u5e38\u7684\u5b58\u6d3b\u3002<\/span><\/p>\n


<\/span><\/p>\n

\u6362\u53e5\u8bdd\u8bf4\uff0c\u8be5\u5b9e\u9a8c\u4e2d\u4f7f\u7528\u7684CRISPR-Cas9\u7cfb\u7edf\u80fd\u591f\u5c06\u90a3\u4e9b\u5b58\u5728p53\u529f\u80fd\u7f3a\u9677\u7684\u7ec6\u80de\u7b5b\u9009\u51fa\u6765\uff0c\u7531\u4e8ep53\u662f\u6291\u764c\u57fa\u56e0\uff0c\u6240\u4ee5\u5c06\u8fd9\u4e9b\u5b58\u5728p53\u529f\u80fd\u7f3a\u9677\u7684\u7ec6\u80de\u5e94\u7528\u4e8e\u75be\u75c5\u6cbb\u7597\u7684\u8bdd\u5c31\u6709\u53ef\u80fd\u5bfc\u81f4\u80bf\u7624\u7684\u5f62\u6210\u3002<\/span><\/span><\/p>\n


<\/span><\/p>\n

\u5f71\u54cd\u6709\u591a\u5927\uff1f<\/span><\/strong><\/p>\n


<\/span><\/p>\n

\u6211\u89c9\u5f97\u8fd9\u4e24\u9879\u7814\u7a76\u6240\u80fd\u4ea7\u751f\u7684\u5f71\u54cd\u5176\u5b9e\u5e76\u6ca1\u6709\u591a\u6570\u6295\u8d44\u8005\u60f3\u8c61\u4e2d\u7684\u90a3\u4e48\u5927\u3002\u56e0\u4e3a\u5373\u4f7fp53\u4f1a\u5f71\u54cdCRISPR-Cas9\u7cfb\u7edf\u5728\u67d0\u4e9b\u7c7b\u578b\u7ec6\u80de\u4e2d\u7684\u57fa\u56e0\u7ec4\u7f16\u8f91\uff0c\u4e5f\u5e76\u4e0d\u610f\u5473\u7740\u6240\u6709\u7684CRISPR\u7597\u6cd5\u90fd\u5b58\u5728\u8fd9\u79cd\u95ee\u9898\u3002<\/span><\/p>\n


<\/span><\/p>\n

\u9996\u5148\uff0c\u81f3\u5c11\u4ece\u76ee\u524d\u6765\u770b\u4e0d\u5b58\u5728\u53cc\u94feDNA\u65ad\u88c2\u7684\u57fa\u56e0\u7ec4\u7f16\u8f91\u4e2d\u4e0d\u4f1a\u5b58\u5728\u8fd9\u4e2a\u95ee\u9898\u3002\u6bd4\u5982\u4e0a\u4e2a\u6708\u5f20\u950b\u3001David Liu\u4ee5\u53caJ Keith Joung\u8054\u5408\u6210\u7acb\u7684Beam Therapeutics\u5c31\u5f88\u96be\u53d7\u5f71\u54cd\u3002\u56e0\u4e3a\u8fd9\u5bb6\u516c\u53f8\u5728\u4f7f\u7528CRISPR\u5bf9\u78b1\u57fa\u8fdb\u884c\u7f16\u8f91\uff0c\u5e76\u4e0d\u6d89\u53caDNA\u7684\u526a\u5207\u548c\u65ad\u88c2\u3002<\/span><\/p>\n

 <\/span><\/p>\n

\u800c\u4e14\u9664\u4e86\u78b1\u57fa\u7f16\u8f91\uff0c\u4f7f\u7528NHEJ\u673a\u5236\u7684CRISPR-Cas9\u7cfb\u7edf\u4e5f\u53ef\u80fd\u4e0d\u4f1a\u53d7\u5f71\u54cd\u3002<\/span>\u7528\u4e8e\u75be\u75c5\u6cbb\u7597\u7684CRISPR\u7597\u6cd5\u4e3b\u8981\u901a\u8fc7\u4e24\u79cd\u65b9\u5f0f\u5bf9DNA\u5e8f\u5217\u8fdb\u884c\u7f16\u8f91\uff0c\u4e00\u79cd\u662f\u5229\u7528\u975e\u540c\u6e90\u672b\u7aef\u8fde\u63a5(NHEJ)\u673a\u5236\u5f62\u6210indel\uff0c\u8fd9\u6837\u53ef\u4ee5\u7834\u574f\u539f\u6709\u57fa\u56e0\u7684\u529f\u80fd\uff0c\u8fd9\u4e5f\u662f\u6cbb\u7597\u9570\u72b6\u7ec6\u80de\u8d2b\u8840\u7684\u7b56\u7565\u3002\u8fd9\u79cd\u7b56\u7565\u4e5f\u53eb\u505a\u57fa\u56e0\u7834\u574f (gene disruption)\u3002\u9664\u4e86\u8fd9\u79cd\u673a\u5236\uff0c\u8fd8\u80fd\u591f\u901a\u8fc7\u540c\u6e90\u4ecb\u5bfc\u7684\u4fee\u590d(HDR)\u6765\u5f15\u5165\u5176\u4ed6\u57fa\u56e0\uff0c\u8fd9\u79cd\u7b56\u7565\u53eb\u505a\u57fa\u56e0\u4fee\u590d (gene correction)\u3002<\/span><\/p>\n

 <\/span><\/p>\n

\u5361\u7f57\u6797\u65af\u5361\u90a3\u7bc7\u6587\u7ae0\u7684\u7b2c\u4e00\u4f5c\u8005EmmaHaapaniemi\u5728\u63a5\u53d7 STAT News <\/em>\u91c7\u8bbf\u65f6\u8bf4\u5728\u4ed6\u4eec\u5b9e\u9a8c\u4e2d\u57fa\u4e8eNHEJ\u4fee\u590d\u673a\u5236\u7684CRISPR-Cas9\uff0c\u5373\u4f7f\u5728p53\u529f\u80fd\u88ab\u6d3b\u5316\u7684\u60c5\u51b5\u4e0b\u4e5f\u4f9d\u7136\u80fd\u591f\u6b63\u5e38\u8fdb\u884c\u57fa\u56e0\u7ec4\u7f16\u8f91\u3002<\/span><\/p>\n


<\/span><\/p>\n

\u56e0\u6b64CRISPR Therapeutics\uff0cEditas Medicine\u8fd9\u4e9b\u516c\u53f8pipeline\u91cc\u57fa\u4e8eNHEJ\u673a\u5236\u7684\u7814\u53d1\u9879\u76ee\u53ef\u80fd\u5e76\u4e0d\u4f1a\u53d7p53\u7684\u5f71\u54cd\u3002\u4f46\u5bf9\u4e8e\u4e00\u4e9bHDR\u76f8\u5173\u7684\u7814\u7a76\u9879\u76ee\u5374\u6709\u53ef\u80fd\u4f1a\u53d7\u5230\u8fd9\u4e9b\u7814\u7a76\u7684\u5f71\u54cd\u3002\u81f3\u4e8e\u5f71\u54cd\u6709\u591a\u5927\u8fd8\u9700\u8981\u8fdb\u884c\u540e\u7eed\u7684\u7814\u7a76\u624d\u80fd\u77e5\u6653\u3002<\/span><\/p>\n


<\/span><\/p>\n

<\/span><\/p>\n

\"Nature\u53d1\u6587\uff0c\u80a1\u4ef7\u5927\u8dcc\uff01p53\u7a76\u7adf\u5982\u4f55\u5f71\u54cdCRISPR\u7684\u672a\u6765\uff1f\"<\/p>\n


<\/p>\n

<\/span><\/p>\n

\"Nature\u53d1\u6587\uff0c\u80a1\u4ef7\u5927\u8dcc\uff01p53\u7a76\u7adf\u5982\u4f55\u5f71\u54cdCRISPR\u7684\u672a\u6765\uff1f\"<\/p>\n

CRISPR Therapeutics\u4ee5\u53caEditas Medicine\u7684pipeline<\/span>
<\/span><\/p>\n


<\/span><\/p>\n

\u9700\u8981\u5f3a\u8c03\u7684\u662f\uff0c\u8fd9\u4e9b\u7814\u7a76\u53ea\u662f\u65e9\u671f\u7684\u4e00\u4e9b\u7814\u7a76\u6570\u636e\uff0c\u76f8\u5173\u516c\u53f8\u7814\u7a76\u4e2d\u6240\u4f7f\u7528\u7684\u7ec6\u80de\u7a76\u7adf\u4f1a\u4e0d\u4f1a\u5728\u8fdb\u884cCRISPR-Cas9\u7f16\u8f91\u7684\u65f6\u5019\u4e5f\u4f1a\u53d7\u5230p53\u7684\u5e72\u6270\u4ece\u800c\u4ea7\u751fp53\u7f3a\u9677\u7ec6\u80de\u7684\u5bcc\u96c6\uff0c\u73b0\u5728\u4e5f\u8fd8\u662f\u672a\u77e5\u6570\u3002<\/span><\/p>\n


<\/span><\/p>\n

\u800c\u4e14\u6211\u89c9\u5f97\u5927\u5bb6\u6240\u62c5\u5fc3\u7684\u8bf1\u53d1\u764c\u75c7\u98ce\u9669\u5f88\u6709\u53ef\u80fd\u53ea\u662f\u5728\u591a\u80fd\u5e72\u7ec6\u80de\u7684\u7814\u7a76\u4e2d\u76f8\u5bf9\u5bb9\u6613\u51fa\u73b0\u3002\u56e0\u4e3a\u8fd9\u5f88\u5927\u7a0b\u5ea6\u4e0a\u662f\u7531\u591a\u80fd\u5e72\u7ec6\u80de\u672c\u8eab\u7684\u7279\u6027\u5bfc\u81f4\u7684\u3002<\/span><\/p>\n


<\/span><\/p>\n

\u5b9e\u9a8c\u4e2d\u5e38\u7528\u7684\u8bf1\u5bfc\u591a\u80fd\u5e72\u7ec6\u80de\u5b58\u5728p53\u7a81\u53d8\u7684\u6bd4\u4f8b\u7279\u522b\u9ad8\uff0c\u80fd\u591f\u8fbe\u52303.5%\uff0c\u800c\u5728\u8f6c\u5f55\u7ec4\u6d4b\u5e8f\u4e2d\u7ecf\u5e38\u4f7f\u7528\u7684\u591a\u80fd\u5e72\u7ec6\u80de\u5176\u7a81\u53d8\u7387\u66f4\u662f\u9ad8\u8fbe29%\u3002\u518d\u7ed3\u5408\u8be5\u7c7b\u7ec6\u80de\u5bf9\u4e8eDNA\u635f\u4f24\u53cd\u5e94\u7684\u654f\u611f\u5ea6\u975e\u5e38\u9ad8\uff0c\u56e0\u6b64<\/span>\u57fa\u4e8e\u8fd9\u4e9b\u7c7b\u578b\u7684\u7ec6\u80de\u4e2d\u51fa\u73b0\u57fa\u56e0\u7f16\u8f91\u4e4b\u540ep53\u7f3a\u9677\u7ec6\u80de\u7684\u5bcc\u96c6\u7684\u73b0\u8c61\u4e5f\u4e0d\u662f\u4ec0\u4e48\u5947\u602a\u7684\u4e8b\u60c5\u3002<\/span><\/p>\n

 <\/span><\/p>\n

\u5176\u5b9e\u65e9\u5728\u53bb\u5e74\u4e03\u6708\u8bfa\u534e\u8fd9\u7bc7\u6587\u7ae0\u7684preprint\u7248\u5c31\u5df2\u7ecf\u5728bioRxiv<\/em>\u4e0a\u7ebf\uff0c\u4f46\u5f53\u65f6\u5e76\u6ca1\u6709\u5f15\u8d77\u5f88\u5927\u7684\u53cd\u54cd\u3002\u800c\u4e4b\u6240\u4ee5\u672c\u5468\u4e00\u56e0\u4e3a\u8fd9\u4e24\u7bc7\u6587\u7ae0\u7684\u4e0a\u7ebfCRISPR\u76f8\u5173\u516c\u53f8\u7684\u80a1\u7968\u96c6\u4f53\u4e0b\u8dcc\uff0c\u5f88\u6709\u53ef\u80fd\u662f\u7531\u4e8e\u5a92\u4f53\u62a5\u9053\u4e2d\u6240\u4f7f\u7528\u7684\u201c\u5f15\u53d1\u764c\u75c7\u201d\u7684\u6807\u9898\u592a\u5f15\u4eba\u6ce8\u76ee\u6240\u5bfc\u81f4\u7684\u3002<\/span><\/p>\n


<\/p>\n

\u5176\u5b9e\u8fd9\u4e5f\u4e0d\u662fCRISPR\u7b2c\u4e00\u6b21\u53d7\u632b\uff0c2017\u5e74\u7684\u65f6\u5019Nature Methods<\/em>\u4e0a\u7684\u4e00\u7bc7\u6587\u7ae0\u62a5\u9053CRISPR\u80fd\u591f\u5f15\u53d1\u5927\u8303\u56f4\u7684\u8131\u9776\u6548\u5e94\uff0c\u4f46\u8fd9\u7bc7\u6587\u7ae0\u5728\u4eca\u5e74\u4e09\u6708\u4efd\u64a4\u7a3f\u3002\u4eca\u5e74\u4e00\u6708bioRxiv\u53c8\u4e0a\u7ebf\u4e86\u4e00\u7bc7\u6587\u7ae0\u79f0\u4eba\u4f53\u80fd\u591f\u5bf9Cas9\u4ea7\u751f\u514d\u75ab\u53cd\u5e94\u3002\u76f8\u540c\u7684\u662f\uff0c\u8fd9\u4e9b\u6587\u7ae0\u7684\u4e0a\u7ebf\u90fd\u80fd\u591f\u5f15\u53d1\u76f8\u5173\u516c\u53f8\u80a1\u4ef7\u7684\u9707\u8361\u3002<\/span><\/p>\n

 <\/span><\/p>\n

\u4f46\u65e0\u8bba\u662f\u8131\u9776\u6548\u5e94\uff0c\u8fd8\u662fp53\uff0c\u6291\u6216\u662f\u514d\u75ab\u53cd\u5e94\uff0c\u8fd9\u4e9b\u90fd\u4e0d\u662f\u79d1\u5b66\u5bb6\u610f\u6599\u4e4b\u5916\u7684\u73b0\u8c61\uff0c\u800c\u4e14\u4e5f\u4e0d\u662f\u4e0d\u5b58\u5728\u76f8\u5e94\u7684\u89e3\u51b3\u65b9\u6848\u3002\u4f46\u663e\u7136\u5f88\u591a\u6295\u8d44\u8005\u5bf9\u6b64\u5e76\u4e0d\u4e86\u89e3\u3002<\/span><\/p>\n

 <\/span><\/p>\n

\u4e0a\u4e2a\u6708Loncar\u5728twitter\u4e0a\u5f04\u4e86\u4e2a\u6295\u7968\uff0c\u95ee\u5927\u5bb6\u4e3a\u4ec0\u4e48\u8ba4\u4e3aCRISPR Therapeutics\u662fCRISPR\u9886\u57df\u6700\u6709\u4ef7\u503c\u7684\u516c\u53f8\uff0c\u591a\u6570\u4eba\u7684\u7b54\u6848\u662f\u56e0\u4e3a\u4ed6\u540d\u5b57\u91cc\u6709CRISPR\u8fd9\u4e2a\u5355\u8bcd\u3002<\/span><\/p>\n


<\/span><\/p>\n

\"Nature\u53d1\u6587\uff0c\u80a1\u4ef7\u5927\u8dcc\uff01p53\u7a76\u7adf\u5982\u4f55\u5f71\u54cdCRISPR\u7684\u672a\u6765\uff1f\"<\/p>\n

<\/span>
<\/p>\n

\u867d\u7136\u542c\u8d77\u6765\u5f88\u641e\u7b11\uff0c\u4f46\u6211\u60f3\u5f88\u5927\u4e00\u90e8\u5206\u6295\u8d44\u8005\u5176\u5b9e\u5e76\u4e0d\u7406\u89e3\u4ec0\u4e48\u662fCRISPR (\u89c1\uff1a<\/span>\u672a\u6765\u5373\u73b0\u5728\uff1aCRISPR\u6280\u672f\u5982\u4f55\u6539\u53d8\u4e16\u754c<\/a><\/span>)\u3002\u7531\u4e8e\u5a92\u4f53\u62a5\u9053\u5f15\u53d1\u7684\u9519\u8bef\u8ba4\u77e5\u4f7f\u5f88\u591a\u4eba\u5bf9\u8fd9\u9879\u6280\u672f\u4ea7\u751f\u4e86\u8fc7\u9ad8\u7684\u9884\u671f\u3002\u8fd9\u5927\u6982\u4e5f\u53ef\u80fd\u662f\u8fd9\u4e9b\u516c\u53f8\u5e02\u503c\u76ee\u524d\u5982\u6b64\u4e4b\u9ad8\u7684\u539f\u56e0\u4e4b\u4e00\u5427\u3002<\/span>
<\/p>\n

 <\/span><\/p>\n

\u867d\u7136CRISPR\u6280\u672f\u4ece2012\u5e74\u8bde\u751f\u4ee5\u6765\u5df2\u7ecf\u5bf9\u57fa\u7840\u79d1\u7814\u9886\u57df\u4ea7\u751f\u4e86\u9769\u547d\u6027\u7684\u5f71\u54cd\uff0c\u4f46\u662f\u60f3\u8981\u5c06\u5176\u5e94\u7528\u4e8e\u75be\u75c5\u7684\u6cbb\u7597\uff0c\u5176\u5b9e\u8fd8\u6709\u975e\u5e38\u957f\u7684\u8def\u8981\u8d70\u3002<\/span><\/p>\n

 <\/span><\/p>\n

\u53bb\u5e74Plos One<\/em>\u4e0a\u6709\u4e00\u7bc7\u6587\u7ae0\u901a\u8fc7\u6570\u636e\u5206\u6790\u53d1\u73b0\uff0c\u4ece\u65b0\u6280\u672f\u51fa\u73b0\u5230\u6709\u76f8\u5173\u836f\u7269\u4e0a\u5e02\u8981\u82b1\u8d39\u5c06\u8fd1\u4e09\u5341\u5e74\u7684\u65f6\u95f4\uff0c\u800cCRISPR\u6280\u672f\u4ece\u8bde\u751f\u5230\u73b0\u5728\u4e5f\u4e0d\u8fc7\u77ed\u77ed\u516d\u5e74\u7684\u65f6\u95f4\u3002<\/span><\/p>\n


<\/span><\/p>\n

<\/span><\/p>\n

\"Nature\u53d1\u6587\uff0c\u80a1\u4ef7\u5927\u8dcc\uff01p53\u7a76\u7adf\u5982\u4f55\u5f71\u54cdCRISPR\u7684\u672a\u6765\uff1f\"<\/p>\n

<\/span>
<\/p>\n

\u5373\u4f7f\u662f\u8fd9\u4e24\u5e74\u5927\u70ed\u7684CAR-T\u6280\u672f\u4ece\u8bde\u751f\u5230\u73b0\u5728\u4e5f\u5df2\u7ecf\u7ecf\u5386\u4e86\u4e8c\u4e09\u5341\u5e74\u7684\u65f6\u95f4(\u89c1\uff1a<\/span>CAR-T\u4f20\u5947<\/a><\/span>)\u3002\u6211\u4e4b\u524d\u5199\u8fc7\u5f88\u591a\u6280\u672f\u7684\u79d1\u666e\u6587\u7ae0\uff0c\u5199\u8fd9\u4e9b\u6587\u7ae0\u7684\u4e00\u4e2a\u5f88\u91cd\u8981\u7684\u76ee\u7684\u5c31\u662f\u63d0\u9192\u5927\u5bb6\u5c06\u65b0\u7684\u6280\u672f\uff0c\u65b0\u7684\u836f\u7269\u60f3\u8981\u5e94\u7528\u4e8e\u4e34\u5e8a\u8fdc\u6bd4\u5f88\u591a\u4eba\u60f3\u8c61\u4e2d\u7684\u8981\u56f0\u96be\uff0cCRISPR\u4e5f\u4e0d\u4f8b\u5916\u3002<\/span><\/p>\n


<\/span><\/p>\n

\u53c2\u8003\u6587\u732e<\/span><\/p>\n

1.<\/span>Nature Medicine <\/span>(<\/span>2018) <\/span><\/span>https:\/\/doi.org\/10.1038\/s41591-018-0050-6<\/span><\/p>\n

2.<\/span><\/span>Nature Medicine <\/span>(<\/span>2018) https:\/\/doi.org\/10.1038\/s41591-018-0049-z<\/span><\/span><\/p>\n

<\/span><\/p>\n


<\/strong><\/span><\/p>\n

Jerry\u4e13\u680f<\/strong><\/span><\/p>\n


<\/strong><\/span><\/p>\n

\u75be\u75c5: <\/span>\u963f\u5c14\u8328\u6d77\u9ed8\u75c5<\/span><\/a> |<\/span> <\/span><\/span>\u6297\u8870\u8001<\/a><\/span> | <\/span><\/span>\u827e\u6ecb\u75c5<\/a><\/span>
<\/p>\n

\u7597\u6cd5: <\/span>\u80bf\u7624\u514d\u75ab\u7597\u6cd5<\/span><\/a> | <\/span>\u57fa\u56e0\u7597\u6cd5<\/a>  | <\/span><\/span>RNA\u5e72\u6270<\/a><\/span> | <\/span>CRISPR<\/span><\/a> | <\/span><\/span>\u7ec6\u80de\u7597\u6cd5<\/a><\/span><\/p>\n

\u836f\u7269:<\/span> <\/span>PARP\u6291\u5236\u5242<\/span><\/a> | <\/span>CETP\u6291\u5236\u5242<\/span><\/a> | <\/span>STING<\/a>\u6fc0\u52a8\u5242 | <\/span><\/span>IDO\u6291\u5236\u5242<\/a> <\/span><\/span>|<\/span>\u6c18\u4ee3\u836f\u7269<\/a><\/span><\/p>\n

\u516c\u53f8\/\u4eba\u7269<\/span>: <\/span>  <\/span>Moderna<\/span><\/a> | <\/span>Vivek Ramaswam<\/a>y<\/span><\/span> <\/span><\/p>\n

\u5176\u4ed6<\/span>: <\/span>\u751f\u7269\u949f\u7b80\u53f2<\/a><\/span> |<\/span> <\/span><\/span>\u81f4\u656c\u970d\u91d1<\/a><\/p>\n


<\/p>\n


<\/p>\n

\"Nature\u53d1\u6587\uff0c\u80a1\u4ef7\u5927\u8dcc\uff01p53\u7a76\u7adf\u5982\u4f55\u5f71\u54cdCRISPR\u7684\u672a\u6765\uff1f\"<\/a>\"Nature\u53d1\u6587\uff0c\u80a1\u4ef7\u5927\u8dcc\uff01p53\u7a76\u7adf\u5982\u4f55\u5f71\u54cdCRISPR\u7684\u672a\u6765\uff1f\"<\/a><\/a>
<\/p>\n

\u25ce \uff08\u70b9\u51fb\u56fe\u7247\u6216\u6587\u5b57\u94fe\u63a5\u5373\u53ef\u9605\u8bfb\uff09<\/span><\/p>\n

\n

\u59cb\u53d1\u4e8e\u5fae\u4fe1\u516c\u4f17\u53f7\uff1a \u533b\u836f\u9b54\u65b9 <\/p>\n<\/blockquote>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

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