{"id":96143,"date":"2019-05-07T00:00:50","date_gmt":"2019-05-17T07:59:02","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/96143\/"},"modified":"2019-05-17T15:59:03","modified_gmt":"2019-05-17T07:59:03","slug":"8b90d9b148","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/96143\/","title":{"rendered":"\u3010\u597d\u6587\u63a8\u8350\u3011\u55dc\u70edS-\u817a\u82f7\u7532\u786b\u6c28\u9178\u5408\u6210\u9176\u7684\u56fa\u5b9a\u5316\u53caS-\u817a\u82f7\u7532\u786b\u6c28\u9178\u7684\u5236\u5907"},"content":{"rendered":"<div class=\"bpp-post-content\">\n<p style=\"white-space: normal;\"><span style=\"background-color: rgb(255, 172, 170);\">\u672a\u7ecf\u6388\u6743\uff0c\u4e0d\u5f97\u8f6c\u8f7d<\/span><br  \/><\/p>\n<p style=\"white-space: normal;text-align: center;\"><strong style=\"font-size: 20px;\">\u55dc\u70edS-\u817a\u82f7\u7532\u786b\u6c28\u9178\u5408\u6210\u9176\u7684\u56fa\u5b9a\u5316\u53caS-\u817a\u82f7\u7532\u786b\u6c28\u9178\u7684\u5236\u5907<\/strong><br  \/><\/p>\n<p style=\"text-align: center;\"><strong>Immobilization of Thermophilic S-Adenosylmethionine Synthetase and Preparation of S-Adenosylmethionine<\/strong><\/p>\n<p style=\"text-align: center;\">\u5316\u6d69\u4e3e1\uff0c\u5434 \u52c71\uff0c\u9ec4\u5b97\u5e861\uff0c\u5f20\u559c\u51682\uff0c\u51af \u519b1*<\/p>\n<p style=\"text-align: center;\">(1. \u4e0a\u6d77\u591a\u7c73\u745e\u751f\u7269\u6280\u672f\u6709\u9650\u516c\u53f8\uff0c\u4e0a\u6d77 201203\uff1b2. \u6b63\u5927\u5929\u6674\u836f\u4e1a\u96c6\u56e2\u80a1\u4efd\u6709\u9650\u516c\u53f8\uff0c\u6c5f\u82cf\u5357\u4eac 210000)<\/p>\n<section  label=\"Powered by 135editor.com\" style=\"white-space: normal;font-size: 16px;font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\n<section class=\"_135editor\"     style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section>\n<section style=\"transform: rotate(0deg);\">\n<section style=\"padding-right: 2em;padding-left: 2em;transform: rotate(0deg);\">\n<section style=\"height: 3em;border-right: 2px solid rgb(36, 96, 142);border-left: 2px solid rgb(36, 96, 142);box-sizing: border-box;\"><\/section>\n<\/section>\n<section style=\"margin-top: -7px;display: flex;justify-content: space-between;align-items: flex-end;\">\n<section style=\"margin-left: 1.7em;\">\n<section   style=\"border-width: 2px;border-style: solid;border-color: rgb(142, 36, 96);width: 12px;height: 12px;background: rgb(36, 96, 142);border-radius: 100%;box-sizing: border-box;color: rgb(255, 255, 255);\"><\/section>\n<\/section>\n<section style=\"margin-right: 1.7em;\">\n<section   style=\"border-width: 2px;border-style: solid;border-color: rgb(142, 36, 96);width: 12px;height: 12px;background: rgb(36, 96, 142);border-radius: 100%;box-sizing: border-box;color: rgb(255, 255, 255);\"><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-top: -10px;padding-right: 5px;padding-bottom: 20px;padding-left: 15px;box-sizing: border-box;\">\n<section style=\"padding-bottom: 8px;border-width: 1px;border-style: solid;border-color: rgb(51, 51, 51);background-color: rgb(36, 96, 142);color: rgb(255, 255, 255);box-sizing: border-box;\">\n<section style=\"margin-top: -10px;margin-right: 10px;margin-left: -10px;padding: 20px 10px 10px;border-width: 1px;border-style: solid;border-color: rgb(51, 51, 51);background-color: rgb(254, 254, 254);box-sizing: border-box;\">\n<section class=\"135brush\"  style=\"padding: 10px 5px;font-size: 14px;line-height: 1.75em;color: rgb(36, 96, 142);box-sizing: border-box;\">\n<p><strong>\u6458\u8981\uff1a<\/strong>\u672c\u7814\u7a76\u4ee5\u56fa\u5b9a\u5316\u9176\u6bd4\u6d3b\u529b\u3001\u9176\u6d3b\u529b\u56de\u6536\u7387\u548c\u53cd\u5e94\u7a33\u5b9a\u6027\u4e3a\u6307\u6807\uff0c\u6bd4\u8f83\u4e86\u6c28\u57fa\u6811\u8102\u548c\u73af\u6c27\u6811\u8102\u56fa\u5b9aS- \u817a\u82f7\u7532\u786b\u6c28\u9178\u5408\u6210\u9176\u7684\u5dee\u522b\u3002\u7ed3\u679c\u663e\u793a\u6c28\u57fa\u6811\u8102\u56fa\u5b9a\u5316\u6548\u679c\u597d\uff0c\u56fa\u5b9a\u5316\u9176\u6bd4\u6d3b\u529b\u4e3a(27.91\u00b11.33)U\/g\uff0c\u56fa\u5b9a\u5316\u9176\u6d3b\u529b\u56de\u6536\u7387\u4e3a(25.78\u00b11.23)\uff05\uff0c\u8fde\u7eed\u53cd\u5e94\u7a33\u5b9a\u6027\u597d\u3002\u4f18\u5316\u540e\u56fa\u5b9a\u5316\u9176\u7684\u53cd\u5e94\u6761\u4ef6\u5982\u4e0b\uff1a\u4e0a\u6837\u4f53\u79ef\u4e3a20 BV\uff0c\u6d41\u901f\u4e3a3 BV\/h\uff0c\u76d0\u7c7b\u578b\u4e3a\u786b\u9178\u94be\u4e0e\u786b\u9178\u9541\uff0c\u4ea7\u7269S- \u817a\u82f7\u7532\u786b\u6c28\u9178\u7684\u6469\u5c14\u8f6c\u5316\u7387\u8fbe(61.78\u00b11.91)\uff05\u3002\u56fa\u5b9a\u5316\u9176\u53cd\u5e94\u6db2\u7ecfDK-1 \u9633\u79bb\u5b50\u4ea4\u6362\u8272\u8c31\u67f1\u7eaf\u5316\u540e\u5f97\u5230\u7eaf\u5ea6\u5927\u4e8e98\uff05\u7684S- \u817a\u82f7\u7532\u786b\u6c28\u9178(SAM)\uff0c\u6536\u7387\u4e3a(71.50\u00b11.61)\uff05\u3002<\/p>\n<p><strong>\u5173\u952e\u8bcd\uff1a<\/strong>S- \u817a\u82f7\u7532\u786b\u6c28\u9178\u5408\u6210\u9176\uff1bS- \u817a\u82f7\u7532\u786b\u6c28\u9178\uff1b\u56fa\u5b9a\u5316\uff1b\u7eaf\u5316<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"white-space: normal;text-align: center;\"><br  \/><\/p>\n<section style=\"padding: 10px;white-space: normal;font-size: 16px;font-family: \u5fae\u8f6f\u96c5\u9ed1;max-width: 100%;line-height: 2em;word-wrap: break-word;word-break: normal;text-align: center;color: rgb(254, 254, 254);background-color: rgb(123, 154, 181);transform: rotateZ(0deg);\"><span class=\"135brush\"  style=\"color: rgb(255, 255, 255);\">\u4ee5\u4e0b\u4e3a\u6587\u7ae0\u8282\u9009<\/span><\/section>\n<p style=\"margin-top: -1px;white-space: normal;font-size: 16px;font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><img class=\"\"      style=\"width: 558px;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/9-1558079943.png\" alt=\"\u3010\u597d\u6587\u63a8\u8350\u3011\u55dc\u70edS-\u817a\u82f7\u7532\u786b\u6c28\u9178\u5408\u6210\u9176\u7684\u56fa\u5b9a\u5316\u53caS-\u817a\u82f7\u7532\u786b\u6c28\u9178\u7684\u5236\u5907\" title=\"\u3010\u597d\u6587\u63a8\u8350\u3011\u55dc\u70edS-\u817a\u82f7\u7532\u786b\u6c28\u9178\u5408\u6210\u9176\u7684\u56fa\u5b9a\u5316\u53caS-\u817a\u82f7\u7532\u786b\u6c28\u9178\u7684\u5236\u5907\"  \/><\/p>\n<p style=\"text-align: center;\"><img class=\"rich_pages\"       style=\"\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/0-1558079943.jpeg\" alt=\"\u3010\u597d\u6587\u63a8\u8350\u3011\u55dc\u70edS-\u817a\u82f7\u7532\u786b\u6c28\u9178\u5408\u6210\u9176\u7684\u56fa\u5b9a\u5316\u53caS-\u817a\u82f7\u7532\u786b\u6c28\u9178\u7684\u5236\u5907\" title=\"\u3010\u597d\u6587\u63a8\u8350\u3011\u55dc\u70edS-\u817a\u82f7\u7532\u786b\u6c28\u9178\u5408\u6210\u9176\u7684\u56fa\u5b9a\u5316\u53caS-\u817a\u82f7\u7532\u786b\u6c28\u9178\u7684\u5236\u5907\"  \/><\/p>\n<p style=\"text-indent: 2em;\">S- \u817a\u82f7\u7532\u786b\u6c28\u9178(S-adenosyl methionine\uff0cSAM\uff0c2) \u662f\u751f\u7269\u4f53\u4e2d\u91cd\u8981\u7684\u4ee3\u8c22\u4e2d\u95f4\u4f53\uff0c\u53c2\u4e0e\u4e86\u751f\u7269\u4f53\u518540 \u591a\u79cd\u4ee3\u8c22\u8fc7\u7a0b[1]\u30022 \u5728\u4e34\u5e8a\u4e0a\u53ef\u7528\u4e8e\u809d\u810f\u635f\u4f24\u3001\u5173\u8282\u708e\u3001\u6291\u90c1\u75c7\u548c\u80c6\u6c41\u6de4\u79ef\u7684\u6cbb\u7597\uff0c\u540c\u65f6\u8fd8\u53ef\u4ee5\u4f5c\u4e3a\u4fdd\u5065\u54c1\u7528\u6765\u9884\u9632\u764c\u75c7\u548c\u51cf\u7f13\u8870\u8001[2\u20143]\u3002\u6211\u56fd\u809d\u75c5\u60a3\u8005\u4f17\u591a\uff0c2 \u5177\u6709\u975e\u5e38\u597d\u7684\u9500\u552e\u524d\u666f\u3002\u76ee\u524d2\u7684\u5de5\u4e1a\u5316\u751f\u4ea7\u65b9\u6cd5\u4e3b\u8981\u662f\u9175\u6bcd\u83cc\u53d1\u9175\u6cd5\uff0c\u8be5\u65b9\u6cd5\u5b58\u5728\u53d1\u9175\u64cd\u4f5c\u4f53\u79ef\u5927\u3001\u6742\u8d28\u542b\u91cf\u9ad8\u548c\u7eaf\u5316\u6b65\u9aa4\u591a\u7b49\u7f3a\u70b9\uff0c\u540c\u65f6\u4f34\u6709\u5e9f\u6c34\u4ea7\u751f\u591a\u548c\u73af\u4fdd\u538b\u529b\u5927\u7684\u95ee\u9898[4]\u3002\u8fd1\u5e74\u6765\u9176\u4fc3\u8f6c\u5316\u6cd5\u751f\u4ea72 \u6210\u4e3a\u7814\u7a76\u70ed\u70b9\uff0c\u4e3b\u8981\u5229\u7528S- \u817a\u82f7\u7532\u786b\u6c28\u9178\u5408\u6210\u9176(S-adenosyl methionine synthetase\uff0c1)\u50ac\u5316L- \u7532\u786b\u6c28\u9178(L-methionine\uff0cL-Met) \u4e0e\u4e09\u78f7\u9178\u817a\u82f7(adenylate triphosphate\uff0cATP) \u5b8c\u6210\uff0c\u4f46\u7531\u4e8e\u6e38\u79bb\u9176\u50ac\u5316\u5b58\u5728\u4ea7\u7269\u4e0d\u6613\u5206\u79bb\u548c\u9176\u96be\u4ee5\u56de\u6536\u7b49\u95ee\u9898\uff0c\u56e0\u6b64\u91c7\u7528\u56fa\u5b9a\u5316\u9176\u6cd5\u662f\u8f83\u597d\u7684\u9009\u62e9[5]\u3002<\/p>\n<p style=\"text-indent: 2em;\">\u672c\u7814\u7a76\u91c7\u7528\u6765\u6e90\u4e8e\u53e4\u83cc\u5c5e\u8a79\u5f0f\u7532\u70f7\u7403\u83cc(Methanococcus jannaschii) \u76841 \u8fdb\u884c\u56fa\u5b9a\u5316\u7814\u7a76\uff0c\u8be5\u9176\u5177\u6709\u6d3b\u6027\u9ad8\u548c\u70ed\u7a33\u5b9a\u6027\u597d\u7684\u4f18\u70b9\uff0c\u65b9\u4fbf\u64cd\u4f5c[6]\u3002<\/p>\n<p><strong>1 \u4eea\u5668\u4e0e\u8bd5\u836f<\/strong><\/p>\n<p><strong>2 \u65b9\u6cd5\u4e0e\u7ed3\u679c<\/strong><\/p>\n<p><strong>2.1 \u9176\u6d3b\u529b\u7684\u6d4b\u5b9a<\/strong><\/p>\n<p style=\"white-space: normal;\"><strong>2.2 1 \u6d53\u7f29\u6db2\u7684\u5236\u5907<\/strong><\/p>\n<p style=\"white-space: normal;\"><strong>2.3 \u56fa\u5b9a\u5316\u9176\u6d3b\u529b\u7684\u6d4b\u5b9a<\/strong><\/p>\n<p style=\"white-space: normal;\"><strong>2.4 2 \u79cd\u6811\u8102\u5171\u4ef7\u56fa\u5b9a\u53161 \u7684\u6bd4\u8f83<\/strong><\/p>\n<p style=\"white-space: normal;\"><strong>2.5 2 \u79cd\u56fa\u5b9a\u5316\u9176\u8fde\u7eed\u53cd\u5e94\u7a33\u5b9a\u6027\u7684\u6bd4\u8f83<\/strong><\/p>\n<p style=\"white-space: normal;\"><strong>2.6 \u56fa\u5b9a\u5316\u9176\u88c5\u67f1\u53cd\u5e94\u6761\u4ef6\u7684\u4f18\u5316<\/strong><\/p>\n<p style=\"white-space: normal;\"><strong>2.7 2 \u7684\u7eaf\u5316<\/strong><\/p>\n<p><strong>3 \u8ba8\u8bba<\/strong><\/p>\n<p style=\"text-indent: 2em;\">\u5173\u4e8e\u9176\u7684\u56fa\u5b9a\u5316\u76ee\u524d\u6ca1\u6709\u7edf\u4e00\u9002\u7528\u7684\u7406\u8bba\u548c\u65b9\u6cd5\u3002\u5df2\u62a5\u9053\u76841 \u7684\u56fa\u5b9a\u5316\u65b9\u6cd5\u5747\u5b58\u5728\u4e00\u4e9b\u95ee\u9898\uff0c\u4f7f\u7528Ni \u79bb\u5b50\u87af\u5408\u67f1\u56fa\u5b9a\u53161\uff0c\u4e0d\u4ec5\u6811\u8102\u4ef7\u683c\u6602\u8d35\uff0c\u800c\u4e143 \u6b21\u8fde\u7eed\u53cd\u5e94\u540e\u56fa\u5b9a\u5316\u9176\u6d3b\u529b\u56de\u6536\u7387\u4ec5\u526939.5\uff05 [8]\u3002\u5c39\u6625\u4e3d\u7b49\u901a\u8fc7\u6d77\u85fb\u9178\u94a0\u548c\u660e\u80f6\u5305\u57cb\u6cd5\u3001\u58f3\u805a\u7cd6\u4e0e\u620a\u4e8c\u919b\u4ea4\u8054\u6cd5\u5f97\u5230\u7684\u56fa\u5b9a\u53161 \u5b58\u5728\u673a\u68b0\u5f3a\u5ea6\u4f4e\u3001\u91cd\u590d\u4f7f\u7528\u8fc7\u7a0b\u4e2d\u5bb9\u6613\u7834\u788e\u4ee5\u53ca\u56fa\u5b9a\u540e\u53cd\u5e94\u65f6\u95f4\u8fc7\u957f\u7b49\u95ee\u9898[9\u201410]\u3002\u5362\u6cd3\u5b87\u7b49\u901a\u8fc7\u79bb\u5b50\u4ea4\u6362\u6811\u8102\u952e\u5408\u6cd5\u5f97\u5230\u56fa\u5b9a\u53161\uff0c\u4e0d\u4ec5\u53cd\u5e94\u65f6\u95f4\u957f\uff0c\u800c\u4e14\u4e0d\u80fd\u8010\u53d7\u9ad8\u79bb\u5b50\u6d53\u5ea6\uff0c\u96be\u4ee5\u5728\u5de5\u4e1a\u751f\u4ea7\u4e2d\u5e94\u7528[11]\u3002\u6c28\u57fa\u6811\u8102\u6210\u672c\u4f4e\u3001\u56fa\u5b9a\u5316\u540e\u7684\u9176\u6bd4\u6d3b\u529b\u548c\u9176\u6d3b\u529b\u56de\u6536\u7387\u8f83\u9ad8\uff0c\u8fde\u7eed\u53cd\u5e94\u7a33\u5b9a\u6027\u597d\u4e14\u673a\u68b0\u5f3a\u5ea6\u9ad8\uff0c\u5177\u6709\u660e\u663e\u7684\u4f18\u52bf\u3002<\/p>\n<p style=\"text-indent: 2em;\">\u672c\u7814\u7a76\u4f7f\u7528\u91cd\u7ec4\u5927\u80a0\u6746\u83cc\u8868\u8fbe1\uff0c\u5e76\u5229\u75281 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