{"id":96172,"date":"2019-05-10T00:00:50","date_gmt":"2019-05-17T07:59:18","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/96172\/"},"modified":"2019-05-17T15:59:19","modified_gmt":"2019-05-17T07:59:19","slug":"7f5a7cd8a7","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/96172\/","title":{"rendered":"\u3010\u597d\u6587\u63a8\u8350\u3011\u8fbe\u6258\u9709\u7d20\u7684\u5206\u79bb\u7eaf\u5316\u5de5\u827a"},"content":{"rendered":"<div class=\"bpp-post-content\">\n<p style=\"white-space: normal;\"><span style=\"background-color: rgb(255, 172, 170);\">\u672a\u7ecf\u6388\u6743\uff0c\u4e0d\u5f97\u8f6c\u8f7d<\/span><br  \/><\/p>\n<p style=\"white-space: normal;text-align: center;\"><strong style=\"font-size: 20px;\">\u8fbe\u6258\u9709\u7d20\u7684\u5206\u79bb\u7eaf\u5316\u5de5\u827a<\/strong><br  \/><\/p>\n<p style=\"white-space: normal;text-align: center;\"><strong>Separation and Purification Process of Daptomycin<\/strong><\/p>\n<p style=\"text-align: center;\">\u90ed\u671d\u6c5f\uff0c\u738b \u8499\uff0c\u5218 \u5fe0\uff0c\u674e\u6625\u5229\uff0c\u5f20\u8d35\u6c11*<\/p>\n<p style=\"text-align: center;\">(\u9c81\u5357\u5236\u836f\u80a1\u4efd\u6709\u9650\u516c\u53f8\uff0c\u5c71\u4e1c\u4e34\u6c82 273400)<\/p>\n<section  label=\"Powered by 135editor.com\" style=\"white-space: normal;font-size: 16px;font-family: \u5fae\u8f6f\u96c5\u9ed1;\">\n<section class=\"_135editor\"     style=\"border-width: 0px;border-style: none;border-color: initial;box-sizing: border-box;\">\n<section>\n<section style=\"transform: rotate(0deg);\">\n<section style=\"padding-right: 2em;padding-left: 2em;transform: rotate(0deg);\">\n<section style=\"height: 3em;border-right: 2px solid rgb(36, 96, 142);border-left: 2px solid rgb(36, 96, 142);box-sizing: border-box;\"><\/section>\n<\/section>\n<section style=\"margin-top: -7px;display: flex;justify-content: space-between;align-items: flex-end;\">\n<section style=\"margin-left: 1.7em;\">\n<section   style=\"border-width: 2px;border-style: solid;border-color: rgb(142, 36, 96);width: 12px;height: 12px;background: rgb(36, 96, 142);border-radius: 100%;box-sizing: border-box;color: rgb(255, 255, 255);\"><\/section>\n<\/section>\n<section style=\"margin-right: 1.7em;\">\n<section   style=\"border-width: 2px;border-style: solid;border-color: rgb(142, 36, 96);width: 12px;height: 12px;background: rgb(36, 96, 142);border-radius: 100%;box-sizing: border-box;color: rgb(255, 255, 255);\"><\/section>\n<\/section>\n<\/section>\n<\/section>\n<section style=\"margin-top: -10px;padding-right: 5px;padding-bottom: 20px;padding-left: 15px;box-sizing: border-box;\">\n<section style=\"padding-bottom: 8px;border-width: 1px;border-style: solid;border-color: rgb(51, 51, 51);background-color: rgb(36, 96, 142);color: rgb(255, 255, 255);box-sizing: border-box;\">\n<section style=\"margin-top: -10px;margin-right: 10px;margin-left: -10px;padding: 20px 10px 10px;border-width: 1px;border-style: solid;border-color: rgb(51, 51, 51);background-color: rgb(254, 254, 254);box-sizing: border-box;\">\n<section class=\"135brush\"  style=\"padding: 10px 5px;font-size: 14px;line-height: 1.75em;color: rgb(36, 96, 142);box-sizing: border-box;\">\n<p><strong>\u6458\u8981\uff1a<\/strong>\u8054\u5408\u5e94\u7528\u53cd\u76f8\u8272\u8c31\u6811\u8102\u548c\u79bb\u5b50\u4ea4\u6362\u6811\u8102\u5206\u79bb\u7eaf\u5316\u8fbe\u6258\u9709\u7d20\uff0c\u6bd4\u8f83\u5404\u6811\u8102\u7684\u8f7d\u6837\u91cf\u3001\u7d2f\u79ef\u6d17\u8131\u56de\u6536\u7387\u548c\u7eaf\u5316\u500d\u6570\u786e\u5b9a\u6811\u8102\u578b\u53f7\u3002\u4f9d\u6b21\u91c7\u7528LP20MM \u53cd\u76f8\u8272\u8c31\u6811\u8102\u3001NM-Q \u79bb\u5b50\u4ea4\u6362\u6811\u8102\u548cXT-30 \u53cd\u76f8\u8272\u8c31\u6811\u8102\u8fdb\u884c\u5206\u79bb\u7eaf\u5316\uff0c\u7eaf\u5316\u5de5\u827a\u53c2\u6570\uff1a\u5c06\u7c97\u5206\u79bb\u53d1\u9175\u6db2\u4e0a\u6837\u4e8eLP20MM \u53cd\u76f8\u8272\u8c31\u6811\u8102\u67f1\uff0c\u752810\uff05\u4e59\u8148\u6eb6\u6db2\u6d17\u8131\uff0c\u6536\u96c6\u8fbe\u6258\u9709\u7d20\u7eaf\u5ea6\u5927\u4e8e80\uff05\u7684\u6d17\u8131\u6db2\uff0c\u4e0a\u6837\u4e8eNM-Q \u79bb\u5b50\u4ea4\u6362\u6811\u8102\u67f1\uff0c\u752810 g\/L \u6c2f\u5316\u94a0\u6eb6\u6db2\u6d17\u8131\uff0c\u6536\u96c6\u8fbe\u6258\u9709\u7d20\u7eaf\u5ea6\u5927\u4e8e90\uff05\u7684\u6d17\u8131\u6db2\uff0c\u4e0a\u6837\u4e8eXT-30 \u53cd\u76f8\u8272\u8c31\u6811\u8102\u67f1\uff0c\u752850\uff05\u4e59\u9187( \u542b\u4e59\u9178\u94f540 mmol\/L) \u6eb6\u6db2\u6d17\u8131\uff0c\u6536\u96c6\u8fbe\u6258\u9709\u7d20\u7eaf\u5ea6\u5927\u4e8e99\uff05\u7684\u6d17\u8131\u6db2\uff0c\u7ecf\u7eaf\u5316\u540e\u8fbe\u6258\u9709\u7d20\u7684\u7eaf\u5ea6\u8fbe\u523099.3\uff05\uff0c\u7eaf\u5316\u6536\u7387\u4e3a57.5\uff05\u3002<\/p>\n<p><strong>\u5173\u952e\u8bcd\uff1a<\/strong>\u8fbe\u6258\u9709\u7d20\uff1b\u5206\u79bb\uff1b\u7eaf\u5316\uff1b\u53cd\u76f8\u8272\u8c31\u6811\u8102\uff1b\u79bb\u5b50\u4ea4\u6362\u6811\u8102<\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"white-space: normal;text-align: center;\"><br  \/><\/p>\n<section style=\"padding: 10px;white-space: normal;font-size: 16px;font-family: \u5fae\u8f6f\u96c5\u9ed1;max-width: 100%;line-height: 2em;word-wrap: break-word;word-break: normal;text-align: center;color: rgb(254, 254, 254);background-color: rgb(123, 154, 181);transform: rotateZ(0deg);\"><span class=\"135brush\"  style=\"color: rgb(255, 255, 255);\">\u4ee5\u4e0b\u4e3a\u6587\u7ae0\u8282\u9009<\/span><\/section>\n<p style=\"margin-top: -1px;white-space: normal;font-size: 16px;font-family: \u5fae\u8f6f\u96c5\u9ed1;\"><img class=\"\"      style=\"width: 558px;\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/0-1558079958.png\" alt=\"\u3010\u597d\u6587\u63a8\u8350\u3011\u8fbe\u6258\u9709\u7d20\u7684\u5206\u79bb\u7eaf\u5316\u5de5\u827a\" title=\"\u3010\u597d\u6587\u63a8\u8350\u3011\u8fbe\u6258\u9709\u7d20\u7684\u5206\u79bb\u7eaf\u5316\u5de5\u827a\"  \/><\/p>\n<p style=\"text-align: center;\"><img class=\"rich_pages\"       style=\"\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2019\/05\/10-1558079958.jpeg\" alt=\"\u3010\u597d\u6587\u63a8\u8350\u3011\u8fbe\u6258\u9709\u7d20\u7684\u5206\u79bb\u7eaf\u5316\u5de5\u827a\" title=\"\u3010\u597d\u6587\u63a8\u8350\u3011\u8fbe\u6258\u9709\u7d20\u7684\u5206\u79bb\u7eaf\u5316\u5de5\u827a\"  \/><\/p>\n<p style=\"white-space: normal;\"><br  \/><\/p>\n<p style=\"text-indent: 2em;\">\u8fbe\u6258\u9709\u7d20(daptomycin\uff0c1) \u662f\u81ea\u73ab\u7470\u5b62\u94fe\u9709\u83cc(Streptomyces roseosporus) \u53d1\u9175\u6db2\u4e2d\u63d0\u53d6\u5f97\u5230\u7684\u4e00\u79cd\u73af\u916f\u80bd\u7c7b\u6297\u751f\u7d20[1]\u30021 \u5177\u6709\u65b0\u9896\u7684\u5316\u5b66\u7ed3\u6784\uff0c\u80fd\u5728\u591a\u4e2a\u65b9\u9762\u7834\u574f\u7ec6\u83cc\u7ec6\u80de\u819c\u7684\u529f\u80fd\uff0c\u8fc5\u901f\u6740\u6b7b\u9769\u5170\u9633\u6027\u83cc[2]\u30021 \u5728\u4e34\u5e8a\u4e0a\u8868\u73b0\u51fa\u5e7f\u8c31\u3001\u836f\u6548\u5feb\u3001\u7ed9\u836f\u91cf\u5c0f\u548c\u4e0d\u826f\u53cd\u5e94\u5c11\u7b49\u4f18\u70b9\uff0c\u88ab\u6279\u51c6\u7528\u4e8e\u6cbb\u7597\u9769\u5170\u9633\u6027\u83cc\u5f15\u8d77\u7684\u590d\u6742\u6027\u76ae\u80a4\u548c\u76ae\u80a4\u7ed3\u6784\u611f\u67d3\uff0c\u4ee5\u53ca\u7531\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc\u5f15\u8d77\u7684\u83cc\u8840\u75c7\u53ca\u53f3\u8def\u5fc3\u5185\u819c\u708e\u75c7[3]\u3002\u6b64\u5916\uff0c1 \u5728\u4f53\u5916\u5bf9\u8010\u7532\u6c27\u897f\u6797\u548c\u5229\u5948\u5511\u80fa\u7684\u83cc\u682a\u4ecd\u5177\u6709\u8f83\u5f3a\u6d3b\u529b[4]\u3002<\/p>\n<p style=\"text-indent: 2em;\">1 \u7684\u5206\u5b50\u7ed3\u6784\u4e2d\u542b\u67094 \u4e2a\u4fa7\u94fe\u7fa7\u9178(3 \u4e2a\u95e8\u51ac\u6c28\u9178\u548c1 \u4e2a\u7532\u57fa\u8c37\u6c28\u9178)\uff0c\u8fd9\u4e9b\u6b8b\u57fa\u7684pKa \u503c\u4e3a3 \uff5e 6\uff0c1 \u7684\u7ed3\u6784\u4e2d\u53c8\u542b\u6709\u8102\u94fe\u548c\u758f\u6c34\u6027\u6c28\u57fa\u9178\uff0c\u51b3\u5b9a\u4e861\u5177\u6709\u4e24\u4eb2\u6027\u7684\u751f\u7269\u5b66\u7279\u6027\u30021 \u5728pH=pI \u6761\u4ef6\u4e0b\u5448\u4e2d\u6027\u5206\u5b50\uff0c\u5728\u5176\u4ed6pH \u503c\u4e0b\u5448\u4e0d\u540c\u7684\u6d3b\u6027\u72b6\u6001[5]\u3002\u672c\u7814\u7a76\u6839\u636e1 \u7684\u7279\u6027\u8054\u5408\u5e94\u7528\u53cd\u76f8\u8272\u8c31\u6cd5\u548c\u79bb\u5b50\u4ea4\u6362\u8272\u8c31\u6cd5\u5206\u79bb\u7eaf\u53161\uff0c\u786e\u5b9a\u4e86\u6700\u4f73\u6811\u8102\u578b\u53f7\u548c\u5de5\u827a\u53c2\u6570\uff0c\u4e3a\u5236\u5907\u9ad8\u7eaf\u5ea6\u76841 \u63d0\u4f9b\u6280\u672f\u53c2\u8003\u3002<\/p>\n<p><strong>1 \u4eea\u5668\u4e0e\u8bd5\u836f<\/strong><\/p>\n<p><strong>2 \u65b9\u6cd5\u4e0e\u7ed3\u679c<\/strong><\/p>\n<p><strong>2.1 \u8272\u8c31\u6761\u4ef6<\/strong><\/p>\n<p style=\"white-space: normal;\"><strong>2.2 \u6eb6\u6db2\u914d\u5236<\/strong><\/p>\n<p><strong>2.3 \u53cd\u76f8\u8272\u8c31\u6811\u8102( \u2160 ) \u7684\u5206\u79bb\u7eaf\u5316<\/strong><\/p>\n<p><strong>2.3.1 \u53cd\u76f8\u8272\u8c31\u6811\u8102( \u2160 ) \u8f7d\u6837\u91cf\u7684\u8003\u5bdf<\/strong><\/p>\n<p style=\"white-space: normal;\"><strong>2.3.2 \u53cd\u76f8\u8272\u8c31\u6811\u8102( \u2160 ) \u7d2f\u79ef\u6d17\u8131\u56de\u6536\u7387\u7684\u8003\u5bdf<\/strong><\/p>\n<p><strong>2.4 \u79bb\u5b50\u4ea4\u6362\u6811\u8102\u7684\u5206\u79bb\u7eaf\u5316<\/strong><\/p>\n<p><strong>2.4.1 \u79bb\u5b50\u4ea4\u6362\u6811\u8102\u8f7d\u6837\u91cf\u7684\u8003\u5bdf<\/strong><\/p>\n<p style=\"white-space: normal;\"><strong>2.4.2 \u79bb\u5b50\u4ea4\u6362\u6811\u8102\u7d2f\u79ef\u6d17\u8131\u56de\u6536\u7387\u7684\u8003\u5bdf<\/strong><\/p>\n<p style=\"white-space: normal;\"><strong>2.5 \u53cd\u76f8\u8272\u8c31\u6811\u8102( \u2161 ) \u7684\u5206\u79bb\u7eaf\u5316<\/strong><\/p>\n<p style=\"white-space: normal;\"><strong>2.5.1 \u53cd\u76f8\u8272\u8c31\u6811\u8102( \u2161 ) \u8f7d\u6837\u91cf\u7684\u8003\u5bdf<\/strong><\/p>\n<p style=\"white-space: normal;\"><strong>2.5.2 \u53cd\u76f8\u8272\u8c31\u6811\u8102( \u2161 ) \u7d2f\u79ef\u6d17\u8131\u56de\u6536\u7387\u7684\u8003\u5bdf<\/strong><\/p>\n<p style=\"white-space: normal;\"><strong>2.6 \u8bd5\u9a8c\u7ed3\u679c\u7684\u9a8c\u8bc1<\/strong><\/p>\n<p><strong>3 \u8ba8\u8bba<\/strong><\/p>\n<p style=\"text-indent: 2em;\">\u76ee\u524d\u56fd\u5916\u4e13\u5229\u62a5\u9053\u76841 \u5206\u79bb\u7eaf\u5316\u65b9\u6cd5\u4e3b\u8981\u662f\u91c7\u7528\u4e01\u9187\u6d78\u63d0\u6216\u4ea4\u66ff\u4f7f\u7528\u9634\u79bb\u5b50\u4ea4\u6362\u8272\u8c31\u3001\u758f\u6c34\u76f8\u4e92\u4f5c\u7528\u8272\u8c31\u548c\u9634\u79bb\u5b50\u4ea4\u6362\u8272\u8c31\u8fdb\u884c\u53cd\u590d\u7eaf\u5316\uff0c\u8fd9\u4e9b\u65b9\u6cd5\u64cd\u4f5c\u590d\u6742\u4e14\u751f\u4ea7\u6210\u672c\u9ad8[6]\u3002\u56fd\u5185\u62a5\u9053\u76841 \u5206\u79bb\u7eaf\u5316\u65b9\u6cd5\u4e3b\u8981\u91c7\u7528\u9634\u79bb\u5b50\u548cC4 \u53cd\u76f8\u7845\u80f6\u590d\u5408\u7269\u4f5c\u4e3a\u590d\u5408\u578b\u53cd\u76f8\u7845\u80f6\u67f1\u8fdb\u884c\u7eaf\u5316\uff0c\u6216\u91c7\u7528\u9634\u79bb\u5b50\u6811\u8102\u8fdb\u884c\u7eaf\u5316\uff0c\u5747\u53d6\u5f97\u4e86\u4e00\u5b9a\u7684\u7eaf\u5316\u6548\u679c\uff0c\u4f46\u6240\u5f971 \u7684\u7eaf\u5ea6\u8f83\u4f4e[7\u20148]\u3002<\/p>\n<p style=\"text-indent: 2em;\">\u672c\u7814\u7a76\u786e\u5b9a\u4e861 \u7684\u5206\u79bb\u7eaf\u5316\u5de5\u827a\uff1a\u4f9d\u6b21\u91c7\u7528LP20MM \u53cd\u76f8\u8272\u8c31\u6811\u8102\u3001NM-Q \u79bb\u5b50\u4ea4\u6362\u6811\u8102\u548cXT-30 \u53cd\u76f8\u8272\u8c31\u6811\u8102\u8fdb\u884c\u5206\u79bb\u7eaf\u5316\u3002\u6700\u4f73\u5de5\u827a\u53c2\u6570\u4e3a\uff1aLP20MM \u53cd\u76f8\u8272\u8c31\u6811\u8102\u67f1\uff0c\u4e0a\u6837\u6db2pH 7.0\uff0c\u6d41\u901f\u4e3a0.8 BV\/h\uff0c\u7528pH 7.0 \u768410\uff05\u4e59\u8148\u6eb6\u6db2\u4ee50.8 BV\/h \u7684\u6d41\u901f\u6d17\u8131\uff1bNM-Q \u79bb\u5b50\u4ea4\u6362\u6811\u8102\u67f1\uff0c\u4e0a\u6837\u6db2pH 7.5\uff0c\u6d41\u901f\u4e3a1 BV\/h\uff0c\u752810 g\/L \u7684\u6c2f\u5316\u94a0\u6eb6\u6db2\u4ee51 BV\/h \u7684\u6d41\u901f\u6d17\u8131\uff1bXT-30 \u53cd\u76f8\u8272\u8c31\u6811\u8102\u67f1\uff0c\u4e0a\u6837\u6db2pH 4.8\uff0c\u6d41\u901f0.5 BV\/h\uff0c\u7528pH 4.0 \u768450\uff05\u4e59\u9187\u6eb6\u6db2( \u542b\u4e59\u9178\u94f540mmol\/L) \u4ee50.5 BV\/h \u7684\u6d41\u901f\u6d17\u8131\u3002\u7ecf\u8fc73 \u6b65\u5206\u79bb\u7eaf\u5316\u5de5\u5e8f\uff0c1 \u7684\u7eaf\u5ea6\u8fbe\u523099.3\uff05\uff0c\u7eaf\u5316\u603b\u56de\u6536\u7387\u4e3a57.5\uff05\u3002\u8be5\u7eaf\u5316\u5de5\u827a\u64cd\u4f5c\u6d41\u7a0b\u7b80\u5355\uff0c\u6d17\u8131\u8d28\u91cf\u597d\uff0c\u663e\u8457\u63d0\u9ad8\u4e861 \u7684\u7eaf\u5ea6\uff0c\u4e3a\u5236\u5907\u9ad8\u7eaf\u5ea6\u76841 \u63d0\u4f9b\u53c2\u8003\u3002<\/p>\n<p style=\"white-space: normal;\"><br  \/><\/p>\n<section style=\"white-space: normal;font-size: 16px;font-family: 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