{"id":98774,"date":"2019-04-22T00:00:50","date_gmt":"2019-05-17T08:16:43","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/98774\/"},"modified":"2019-05-17T16:16:44","modified_gmt":"2019-05-17T08:16:44","slug":"0f57b1a6a2","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/98774\/","title":{"rendered":"\u80bf\u7624\u4ee3\u8c22\u91cd\u6392\u4e0e\u80bf\u7624\u8010\u836f\u76f8\u5173\u6027\u7684\u7814\u7a76\u8fdb\u5c55"},"content":{"rendered":"
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Advances in studies on relationship between tumor metabolic rearrangement and tumor chemotherapy resistance<\/span><\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u836f\u7269\u8bc4\u4ef7\u7814\u7a76\uff0cDrug Evaluation Research <\/p>\n

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1<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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1.1 \u7cd6\u9175\u89e3\u901a\u8def\u7684\u6539\u53d8<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u8fd9\u79cd\u4ee3\u8c22\u9002\u5e94\u4f34\u968f\u7740\u7ec6\u80de\u5bf9\u963f\u9709\u7d20\u548c\u963f\u7cd6\u80de\u82f7\uff08Ara-C\uff09\u7684\u654f\u611f\u6027\u964d\u4f4e\uff3b7\uff3d\u3002<\/span><\/p>\n

\u80bf\u7624\u60a3\u8005\u4e2d\uff0c\u9ad8\u4e73\u9178\u6c34<\/span>\u5e73\u4e0e\u5934\u9888\u764c\u3001\u76f4\u80a0\u817a\u764c\u548c\u5bab\u9888\u764c\u7684\u8f6c\u79fb\u5448\u6b63\u76f8\u5173\u3002<\/span>\u4e73\u9178\u8fd8\u589e\u5f3aMCF-7 \u4eba\u4e73\u817a\u764c\u7ec6\u80de\u7684\u514b\u9686\u5f62\u6210\u80fd\u529b<\/span>\u5e76\u8bf1\u5bfc\u6837\u76f8\u5173\u7684\u9057\u4f20\u7279\u5f81\uff3b8\uff3d\u3002<\/span><\/p>\n

\u591a\u805a\u5627\u5576\u533a\u7ed3\u5408\u86cb\u767d1\uff08polypyrimidine tract binding protein 1\uff0cPTBP1\uff09\u662f\u7cd6\u9175\u89e3\u7684\u8c03\u8282\u56e0\u5b50\uff0c\u7814<\/span>\u7a76\u53d1\u73b0\u901a\u8fc7\u6572\u9664PTBP1 \u8c03\u63a7\u7cd6\u9175\u89e3\uff0c\u514b\u670d\u4e86\u8010\u836f\u7ed3<\/span>\u80a0\u764c\u7ec6\u80de\u5bf9\u957f\u6625\u65b0\u78b1\u548c\u5965\u6c99\u5229\u94c2\u7684\u8010\u836f\uff3b9\uff3d\u3002<\/span><\/p>\n

\u5df1\u7cd6\u6fc0<\/span>\u91762\uff08HK2\uff09\u662f\u50ac\u5316\u7cd6\u4ee3\u8c22\u7684\u7b2c\u4e00\u4e2a\u9650\u901f\u6b65\u9aa4\uff0c\u4fc3\u8fdb<\/span>ATP \u7684\u7acb\u5373\u5229\u7528\u3002<\/span><\/p>\n

HK2 \u6291\u5236\u5242\u5728\u51e0\u79cd\u4f53\u5916\u6a21\u578b\u4e2d<\/span>\u8868\u73b0\u51fa\u4e86\u4e00\u5b9a\u7684\u9006\u8f6c\u8010\u836f\u7684\u6548\u679c\u3002\u4f7f\u75282-DG\u4f5c\u4e3a<\/span>HK2 \u6291\u5236\u5242\u81f4\u654f\u80bf\u7624\u7ec6\u80de\u540e\uff0c\u53ef\u63d0\u9ad8\u7ec6\u80de\u6bd2\u836f\u7269\u963f<\/span>\u9709\u7d20\u6216\u7d2b\u6749\u9187\u7684\u7597\u6548\uff3b10\uff3d\u3002<\/p>\n

\u7814\u7a76\u53d1\u73b0\u6c89\u9ed8M2 \u578b\u4e19\u916e\u9178\u6fc0\u9176\uff08pyruvate kinase isozymes M2\uff0cPKM2\uff09\u53ef\u5bfc<\/span>\u81f4ATP \u5408\u6210\u51cf\u5c11\uff0c\u589e\u52a0\u591a\u897f\u4ed6\u8d5b\u5728A549 \u80ba\u764c\u7ec6\u80de<\/span>\u5185\u79ef\u7d2f\u548c\u6297\u80bf\u7624\u4f5c\u7528\uff3b11\uff3d\u3002<\/p>\n

\u4e73\u9178\u8131\u6c22\u9176-A\uff08lactatedehydrogenase A\uff0cLDHA\uff09\u50ac\u5316\u4e19\u916e\u9178\u548c\u4e73\u9178\u7684\u76f8\u4e92<\/span>\u8f6c\u5316\uff0c\u5728\u4eba\u7c7b\u80bf\u7624\u4e2d\u4e0a\u8c03\uff0c\u5e76\u4e14\u4e0e\u80bf\u7624\u4fb5\u88ad\u6027\u76f8\u5173\u3002<\/span><\/p>\n

\u5728\u81f4\u764c\u57fa\u56e0K-RAS \u6216EGFR \u9a71\u52a8\u7684NSCLC \u5c0f\u9f20\u6a21\u578b\u4e2d\uff0cLDHA\u7684\u5931\u6d3b\u4f7f\u80bf\u7624\u53d1\u751f\u51cf\u5c11\u5e76\u5bfc\u81f4\u75be\u75c5\u6d88<\/span>\u9000\u3002<\/span><\/p>\n

\u540c\u65f6\uff0cLDHA\u7684\u5c0f\u5206\u5b50\u6291\u5236\u5242\u51cf\u5c11\u4e86RAS \u8bf1\u5bfc<\/span>\u7684\u80bf\u7624\u6570\u91cf\uff0c\u4e5f\u8bf4\u660eLDHA\u5bf9\u4e8e\u4f9d\u8d56\u4e8e\u80bf\u7624<\/span>\u7684\u8010\u836f\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\u662f\u53ef\u884c\u7684\u6cbb\u7597\u9776\u6807\uff3b12\uff3d\u3002<\/span><\/p>\n

\u7ebf\u7c92\u4f53\u9499\u6444\u5165\u86cb\u767d1\uff08mitochondrial calcium uptake<\/span>1\uff0cMICU1 \/ CBARA1\uff09\u9a71\u52a8\u5375\u5de2\u764c\u4e2d\u7684\u6709\u6c27\u7cd6\u9175<\/span>\u89e3\u3002<\/span><\/p>\n

\u5728\u4f53\u5916\u6c89\u9ed8MICU1 \u589e\u52a0\u6c27\u6d88\u8017\uff0c\u51cf\u5c11\u4e73\u9178\u4ea7<\/span>\u751f\uff0c\u6291\u5236\u7ec6\u80de\u751f\u957f\uff0c\u6291\u5236\u5375\u5de2\u764c\u7ec6\u80de\u7684\u8fc1\u79fb\u548c\u4fb5\u88ad\u3002<\/span>\u4f53\u5185\u6c89\u9ed8MICU1 \u6291\u5236\u80bf\u7624\u751f\u957f\uff0c\u589e\u52a0\u987a\u94c2\u836f\u6548\u548c\u603b<\/span>\u4f53\u5b58\u6d3b\u7387\uff3b13\uff3d\u3002<\/span><\/p>\n

\u4f7f\u7528shRNA \u6216\u5355\u7fa7\u9178\u8f6c\u8fd0\u86cb\u767d1\uff08monocarboxylate transporter 1\uff0cMCT1\uff09\u6291\u5236\u5242\u6765<\/span>\u6291\u5236MCT1\uff0c\u5728\u4f53\u5185\u5916\u5305\u62ec\u5728\u9aa8\u8089\u7624\u7684\u539f\u4f4d\u6a21\u578b\u4e2d<\/span>\u5747\u5ef6\u8fdf\u80bf\u7624\u751f\u957f\uff0c\u4e5f\u663e\u8457\u6291\u5236\u4e86\u9aa8\u8089\u7624\u7684\u8f6c\u79fb\u548c\u4fb5<\/span>\u88ad\u3002<\/span><\/p>\n

\u540c\u65f6\uff0c\u9776\u5411MCT1 \u6781\u5927\u5730\u589e\u5f3a\u4e86\u4eba\u9aa8\u8089\u7624\u7ec6\u80de<\/span>\u5bf9\u5316\u7597\u836f\u7269ADR \u7684\u654f\u611f\u6027\u3002\u514d\u75ab\u5316\u5b66\u5206\u6790\u663e\u793a<\/span>MCT1 \u662f\u9aa8\u8089\u7624\u60a3\u8005\u7684\u9633\u6027\u9884\u540e\u6807\u5fd7\u7269\uff3b14\uff3d\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n

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1\uff0e2 \u78f7\u9178\u620a\u7cd6\u9014\u5f84\uff08Pentose phosphate pathway \uff0c<\/span>PPP\uff09\u901a\u8def\u7684\u6539\u53d8<\/span><\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n

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PPP \u662f\u78f7\u9178\u620a\u7cd6\u548c\u6838\u7cd6\u6838\u82f7\u9178\u5408\u6210\u6240\u5fc5\u9700\u7684\u9014\u5f84\uff0c\u4e5f\u662fNADPH \u7684\u4e3b\u8981\u6765\u6e90\u3002<\/span><\/p>\n

PPP \u5728\u5e2e\u52a9\u504f\u597d\u7cd6<\/span>\u9175\u89e3\u7684\u80bf\u7624\u7ec6\u80de\u6ee1\u8db3\u5176\u5408\u6210\u4ee3\u8c22\u9700\u6c42\u5e76\u5bf9\u6297\u6c27\u5316<\/span>\u5e94\u6fc0\u65b9\u9762\u8d77\u7740\u5173\u952e\u7684\u4f5c\u7528\u3002<\/span><\/p>\n

\u6c27\u5316\u5e94\u6fc0\u3001\u7535\u79bb\u8f90\u5c04\u6216<\/span>\u5316\u5b66\u7597\u6cd5\u53ef\u5f15\u8d77ROS \u6c34\u5e73\u5347\u9ad8\uff0c\u800c\u80bf\u7624\u7ec6\u80de\u901a\u8fc7\u589e<\/span>\u52a0PPP \u6d3b\u6027\u4ee5\u62b5\u6297\u6c27\u5316\u5e94\u6fc0\u8bf1\u5bfc\u7684\u7ec6\u80de\u51cb\u4ea1\u3002<\/span><\/p>\n

\u4f8b<\/span>\u5982\uff0c\u5728\u7ec6\u80de\u66b4\u9732\u4e8e\u7535\u79bb\u8f90\u5c04\u5f15\u8d77\u6c27\u5316\u5e94\u6fc0\u540e\uff0c\u7ec6\u80de<\/span>\u4e2d\u8461\u8404\u7cd6-6-\u78f7\u9178\u8131\u6c22\u9176\uff08G6PD\uff09\u589e\u52a0\u4f7f\u7ec6\u80de\u514d\u53d7\u8f90<\/span>\u5c04\u635f\u4f24\uff3b15\uff3d\u3002<\/span><\/p>\n

\u6709\u7814\u7a76\u8868\u660e\uff0c\u5728\u975e\u836f\u7269\u8bf1\u5bfc\u7684\u8010\u836f\u7ec6\u80de<\/span>\u6a21\u578b\u4e2d\uff0cPPP \u4e5f\u8d77\u5230\u4e86\u5173\u952e\u4f5c\u7528\u3002<\/span><\/p>\n

\u5df2\u77e5\uff0cMCF-7 \u5f62\u6210<\/span>3D \u7403\u4f53\u7ec6\u80de\u7684\u8fc7\u7a0b\u4e2d\uff0c\u901a\u8fc7P-gp \u7684\u8fc7\u8868\u8fbe\u4ecb\u5bfc\u8010<\/span>\u836f\u4ea7\u751f\uff0c\u5bf9ADR \u8010\u836f\u6027\u9010\u6e10\u589e\u52a0\u3002<\/span><\/p>\n

\u540c\u65f6\uff0c\u4ee3\u8c22\u7ec4\u7ed3<\/span>\u679c\u663e\u793aPPP \u4e3b\u8981\u4ee3\u8c22\u7269\u5728\u8010\u836f\u6027\u5f62\u6210\u7684\u8fc7\u7a0b\u4e2d\u663e\u8457<\/span>\u51cf\u5c11\uff0cG6PD\u8868\u8fbe\u548c\u6d3b\u6027\u5747\u663e\u8457\u964d\u4f4e\u3002\u57282D MCF-7<\/span>\u7ec6\u80de\u4e2d\u6c89\u9ed8G6PD\uff0c\u4f34\u968f\u7740ROS \u7684\u589e\u52a0\uff0cNADPH\/<\/span>NADP+\u548cGSH\/GSSG\u6bd4\u503c\u4e5f\u964d\u4f4e\uff0cP-gp \u57fa\u56e0\u548c\u86cb\u767d<\/span>\u6c34\u5e73\u663e\u8457\u63d0\u9ad8\u3002<\/span><\/p>\n

\u540c\u65f6\uff0c\u5f533D MCF-7 \u7ec6\u80de\u8fc7\u8868\u8fbe<\/span>G6PD \u65f6\uff0cROS \u6c34\u5e73\u964d\u4f4e\uff0cNADPH\/NADP+\u548cGSH\/<\/span>GSSG\u5347\u9ad8\uff0cP-gp \u7684\u57fa\u56e0\u548c\u86cb\u767d\u6c34\u5e73\u5747\u663e\u8457\u964d\u4f4e\uff3b16\uff3d\u3002<\/span><\/p>\n

\u76f8\u6bd4\u4e8e\u6bd4\u836f\u7269\u654f\u611f\u7684\u80bf\u7624\uff0c\u5927\u591a\u6570\u591a\u836f\u8010\u836f\u80bf<\/span>\u7624\u4e2dPPP \u6c27\u5316\u5206\u652f\u66f4\u6d3b\u8dc3\u3002<\/span><\/p>\n

\u4f8b\u5982\uff0c\u5728\u5c0f\u9f20\u6dcb\u5df4\u7ec6\u80de<\/span>\u767d\u8840\u75c5P388 \u8010\u836f\u7ec6\u80de\u4e2d\uff0cPPP \u7684\u6d3b\u6027\u589e\u52a0\u4e862 \u500d\uff0c\u8fd9<\/span>\u662f\u7531\u4e8e\u4e24\u4e2aNADPH \u8131\u6c22\u9176G6PD \u548c6PGD \u7684\u6d3b\u6027<\/span>\u63d0\u9ad8\u4e8640%\uff3b17\uff3d\u3002<\/span><\/p>\n

\u76f8\u4f3c\u7684\uff0c\u4ebaT \u6dcb\u5df4\u6bcd\u7ec6\u80de\u6837\u7ec6\u80de\u7cfb<\/span>CEM\u5728\u957f\u6625\u78b1\u7684\u8bf1\u5bfc\u4e0b\u4ea7\u751f\u7684CEM-VBL100 \u8010\u836f<\/span>\u7ec6\u80de\u6bd4\u654f\u611f\u7684\u7ec6\u80de\u5177\u6709\u66f4\u9ad8\u7684G6PD \u6d3b\u6027\u548cGSH<\/span>\u6c34\u5e73\uff3b18\uff3d\u3002<\/span><\/p>\n

\u5728PPP \u4e0a\u8c03\u7684\u60c5\u51b5\u4e0b\uff0cGSH \u6c34\u5e73\u589e\u52a0\uff0c\u8fd9<\/span>\u4f7f\u5f97K-Ras \u7a81\u53d8\u7684\u80ba\u764c\u548c\u80f0\u817a\u764c\u5728\u751f\u957f\u589e\u6b96\u65b9\u9762\u66f4<\/span>\u6709\u4f18\u52bf\uff0c\u800c\u4e14\u8010\u836f\u6027\u66f4\u5f3a\uff3b19\uff3d\u3002\u56e0\u6b64\uff0c\u4e00\u4e9bPPP \u901a\u8def\u6291<\/span>\u5236\u5242\u4e0e\u653e\u5316\u7597\u836f\u8054\u7528\u53ef\u589e\u5f3a\u7597\u6548\u3002<\/span><\/p>\n

\u6bd4\u5982\uff0cDHEA\u4f5c<\/span>\u4e3aG6PD\u7684\u975e\u7ade\u4e89\u6027\u6291\u5236\u5242\u5728\u4f53\u5916\u660e\u663e\u6291\u5236PPP \u901a<\/span>\u91cf\u548cNADPH \u4ea7\u751f\uff0c\u589e\u5f3a\u4e86\u7d2b\u6749\u9187\u5bf9MDA-MB-231<\/span>\u4e73\u817a\u764c\u7ec6\u80de\u7684\u6cbb\u7597\u6f5c\u529b\uff3b20\uff3d\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n

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1.3 \u4e09\u7fa7\u9178\u5faa\u73af\uff08tricarboxylic acid cycle TCA <\/span><\/strong>cycle\uff09\u901a\u8def\u7684\u6539\u53d8<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u867d\u7136\u80bf\u7624\u7ec6\u80de\u66f4\u4f9d\u8d56\u7cd6\u9175\u89e3\u9014\u5f84\uff0c\u4f46\u662f\u6709\u7814\u7a76\u663e\u793a\u5316\u7597\u836f\u7269\u6740\u4f24\u80bf\u7624\u7ec6\u80de\u540e\uff0c\u4f7f\u5f97\u80bf\u7624\u7ec6\u80de\u5fc5\u987b<\/span>\u964d\u4f4e\u5176\u589e\u6b96\u901f\u5ea6\u5e76\u8f6c\u5411OXPHOS \u4ee3\u8c22\u3002<\/span><\/p>\n

\u5229\u7528\u8f6c\u5f55<\/span>\u7ec4\u5b66\u65b9\u6cd5\u8fdb\u884c\u901a\u8def\u5bcc\u96c6\u5206\u6790\uff0c\u663e\u793a\u4f7f\u7528\u5316\u7597\u836f\u7269\u540e<\/span>\u5f02\u79cd\u79fb\u690d\u7624\u4e2d\u53c2\u4e0eOXPHOS \u548c\u7ebf\u7c92\u4f53\u751f\u7269\u8f6c\u5316\u7684<\/span>\u57fa\u56e0\u663e\u8457\u4e0a\u8c03\u3002<\/span><\/p>\n

\u5bf9\u60a3\u8005\u6765\u6e90\u7684\u7ed3\u80a0\u5c42\u57f9\u517b\u7269\u7ed9\u4e88<\/span>\u5965\u6c99\u5229\u94c2\u548c5-FU\u53ef\u5bfc\u81f4\u7ebf\u7c92\u4f53\u751f\u7269\u91cf\u589e\u52a0\u3001\u547c\u5438\u94fe<\/span>\u9176\u8868\u8fbe\u589e\u52a0\u3001\u8017\u6c27\u91cf\u589e\u52a0\uff0c\u8868\u660e\u5316\u7597\u8bf1\u5bfc\u80bf\u7624\u7ec6\u80de<\/span>\u4ee3\u8c22\u4ece\u7cd6\u9175\u89e3\u5411OXPHOS \u8f6c\u53d8\u3002\u8fd9\u662f\u7531\u7ec4\u86cb\u767d\u53bb<\/span>\u4e59\u9170\u9176-1\uff08Sirtuin 1\uff09\u53ca\u5176\u5e95\u7269\uff0c\u8f6c\u5f55\u8f85\u56e0\u5b50PGC1\u03b1<\/span>\u4ecb\u5bfc\u7684\u3002<\/span><\/p>\n

\u6572\u9664Sirtuin 1 \u7f16\u7801\u7684\u57fa\u56e0SIRT1 \u6216PGC1\u03b1<\/span>\u540e\u53ef\u963b\u6b62\u5316\u7597\u836f\u5f15\u8d77\u7684\u80bf\u7624\u4ee3\u8c22\u5411OXPHOS \u8f6c\u53d8\uff0c<\/span>\u540c\u65f6\u4e5f\u4f7f\u5f97\u75c5\u4eba\u6765\u6e90\u7684\u7ed3\u80a0\u764c\u7ec6\u80de\u53ca\u5176\u5f02\u79cd\u79fb\u690d<\/span>\u7624\u5bf9\u836f\u7269\u6cbb\u7597\u654f\u611f\uff3b21\uff3d\u3002<\/span><\/p>\n

\u7814\u7a76\u53d1\u73b0\uff0c\u5229\u7528\u9c7c\u85e4\u916e\uff08\u7ebf\u7c92\u4f53\u7535\u5b50\u4f20\u9012\u590d\u5408\u7269I \u7684\u4e00\u79cd\u7279\u5f02\u6027\u6291\u5236\u5242\uff09\u5bf9\u767d\u8840\u75c5\u7ec6\u80deHL-60 \u7684<\/span>\u7ebf\u7c92\u4f53\u547c\u5438\u8fdb\u884c\u6291\u5236\uff0c\u4ea7\u751f\u7684\u8fd9\u79cd\u547c\u5438\u7f3a\u9677\u578b\u7ec6\u80de<\/span>\u5bf9\u4e34\u5e8a\u5e38\u7528\u7684\u51e0\u79cd\u6297\u80bf\u7624\u836f\u7269\u5305\u62ecAra-C\u3001ADR\u3001\u7d2b<\/span>\u6749\u9187\u548c\u957f\u6625\u65b0\u78b1\u7684\u654f\u611f\u6027\u660e\u663e\u4f4e\u4e8e\u4eb2\u672c\u7ec6\u80de\uff3b7\uff3d\u3002<\/span><\/p>\n

\u4e00<\/span>\u4e9b\u7814\u7a76\u6307\u51fa\uff0c\u80bf\u7624\uff08cancer stem cell\uff0cCSC\uff09\u5bf9<\/span>\u80bf\u7624\u8010\u836f\u548c\u80bf\u7624\u590d\u53d1\u8d77\u5230\u4e86\u91cd\u8981\u4f5c\u7528\uff0c\u4f8b\u5982\uff0c\u7ed3\u80a0<\/span>CSC\u7ebf\u7c92\u4f53\u8f83\u5c11\uff0c\u5e76\u4e14ABT-737 \u589e\u52a0\u7ebf\u7c92\u4f53\u4f7f\u7ed3\u80a0<\/span>CSC \u5bf9\u6cbb\u7597\u654f\u611f\uff3b22\uff3d\u3002\u6709\u7814\u7a76\u89c2\u5bdf\u5230\u7d2b\u6749\u9187\u8010\u836f\u7ec6<\/span>\u80de\u4e2d\u67e0\u6aac\u9178\u6c34\u5e73\u964d\u4f4e\uff0c\u5e76\u4e14\u5b58\u5728\u7ebf\u7c92\u4f53\u547c\u5438\u7f3a\u9677\u3002<\/span><\/p>\n

\u4e8c\u6c2f\u4e59\u9178\u76d0\u7279\u5f02\u6027\u4f5c\u7528\u4e8e\u7ebf\u7c92\u4f53\u547c\u5438\u7f3a\u9677\u7ec6\u80de\uff0c\u8bf1<\/span>\u5bfc\u67e0\u6aac\u9178\u76d0\u79ef\u7d2f\uff0c\u4ece\u800c\u6291\u5236\u7cd6\u9175\u89e3\u548c\u7cd6\u86cb\u767d\uff0c\u56e0\u6b64<\/span>\u4e8c\u6c2f\u4e59\u9178\u76d0\u9006\u8f6c\u7d2b\u6749\u9187\u8010\u836f\uff3b23\uff3d\u3002<\/span><\/p>\n

\u7ebf\u7c92\u4f53<\/span>DNA\uff08mitochondria DNA\uff0cmtDNA\uff09\u51cf\u5c11\u7684\u7ec6\u80de\u5177\u6709<\/span>\u589e\u5f3a\u7684\u8461\u8404\u7cd6\u6444\u53d6\u80fd\u529b\u5e76\u4ea7\u751f\u9ad8\u6c34\u5e73\u7684\u4e73\u9178\uff0c\u5e76\u4e14<\/span>HK2 \u548c\u78f7\u9178\u679c\u7cd6\u6fc0\u9176\uff08phosphofructokinase\uff0cPFK\uff09<\/span>\u7684\u6d3b\u6027\u589e\u52a0\u3002<\/span><\/p>\n

\u8fd9\u8868\u660emtDNA\u964d\u4f4e\u7684\u7ec6\u80de\u8f6c\u5411\u6709\u6c27<\/span>\u7cd6\u9175\u89e3\uff0c\u6709\u5229\u4e8e\u7ec6\u80de\u751f\u5b58\u3002<\/span><\/p>\n

\u6b64\u5916\uff0cmtDNA\u964d\u4f4e\u7684\u7ec6<\/span>\u80de\u5bf95-\u6c1f\u5c3f\u5627\u5576\uff085-FU\uff09\u548c\u5965\u6c99\u5229\u94c2\u8bf1\u5bfc\u7684\u7ec6\u80de\u51cb<\/span>\u4ea1\u5177\u6709\u9ad8\u5ea6\u8010\u836f\u6027\uff0c\u5e76\u4e14\u8fd9\u79cd\u8010\u836f\u6027\u5728\u6062\u590dmtDNA<\/span>\u542b\u91cf\u540e\u662f\u53ef\u9006\u7684\u3002<\/span><\/p>\n

\u4f5c\u8005\u8fd8\u53d1\u73b0Akt\/mTOR \u901a\u8def\u5728<\/span>mtDNA \u964d\u4f4e\u7684\u7ec6\u80de\u4e2d\u88ab\u6fc0\u6d3b\u3002\u8be5\u9014\u5f84\u53ef\u80fd\u5728<\/span>mtDNA\u964d\u4f4e\u7ec6\u80de\u7684\u8010\u836f\u6027\u4e2d\u53d1\u6325\u91cd\u8981\u4f5c\u7528\uff0c\u5f53\u8be5\u9014<\/span>\u5f84\u88ab\u6291\u5236\u65f6\u836f\u7269\u654f\u611f\u6027\u5f97\u4ee5\u6062\u590d\uff3b24\uff3d\u3002<\/span><\/p>\n

mtDNA\u7a81\u53d8<\/span>\u7684\u80f0\u817a\u764c\u7ec6\u80de\u7cfbCFPAC-1 \u548cCAPAN-2 \u5bf95-FU\u548c\u987a<\/span>\u94c2\u5747\u5177\u6709\u8010\u836f\u6027\uff3b25\uff3d\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n

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1.4 \u8102\u8d28\u4ee3\u8c22\u901a\u8def\u7684\u6539\u53d8<\/span><\/strong>
<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u76f8\u8f83\u4e8e\u7cd6\u4ee3\u8c22\uff0c\u5bf9\u80bf\u7624\u7ec6\u80de\u4e2d\u8102\u80aa\u9178\uff08fatty acid\uff0cFA\uff09\u4ee3\u8c22\u7684\u5173\u6ce8\u8f83\u5c11\u3002<\/span><\/p>\n

\u4f46\u8fd1\u51e0\u5e74\u8d8a\u6765\u8d8a\u591a\u7684\u4eba<\/span>\u8ba4\u8bc6\u5230\u9ad8\u8102\u5408\u6210\u662f\u80bf\u7624\u7684\u53e6\u4e00\u4e2a\u91cd\u8981\u7684\u4ee3\u8c22\u7279\u5f81\u3002<\/span><\/p>\n

\u8102\u8d28\u4f5c\u4e3a\u7b2c\u4e8c\u4fe1\u4f7f\u548c\u6fc0\u7d20\u4e5f\u53ef\u4ee5\u5728\u4fe1\u53f7\u4f20\u9012\u4e2d\u626e<\/span>\u6f14\u91cd\u8981\u7684\u89d2\u8272\u3002\u8102\u8d28\u4ee3\u8c22\u7684\u53d8\u5316\u53ef\u5f71\u54cd\u7ec6\u80de\u751f\u957f\u3001<\/span>\u589e\u6b96\u3001\u5206\u5316\u548c\u80fd\u52a8\u6027\uff3b26\uff3d\u3002<\/span><\/p>\n

<\/span>\u8102\u80aa\u9178\u6c27\u5316\uff08fatty acid oxidation\uff0cFAO\uff09\u5df2\u7ecf\u88ab<\/span>\u8bc1\u660e\u4e0e\u5316\u7597\u8010\u836f\u6709\u5173\u3002\u8010\u836f\u80bf\u7624\u7ec6\u80de\u8868\u73b0\u51fa\u5bf9<\/span>FAO\u7684\u4f9d\u8d56\uff0c\u8fd9\u53ef\u80fd\u6e90\u4e8eFAO\u4ee3\u507f\u6027\u589e\u52a0\u7ec6\u80deATP<\/span>\u548cNADPH\uff0c\u5e76\u4ea7\u751f\u4e2d\u95f4\u4ea7\u7269\u4ee5\u652f\u6301\u7ec6\u80de\u5feb\u901f\u751f\u957f\uff0c<\/span>\u6d88\u9664\u6f5c\u5728\u7684\u6709\u6bd2\u8102\u8d28\uff0c\u6291\u5236\u7ec6\u80de\u51cb\u4ea1\u9014\u5f84\uff3b27\uff3d\u3002<\/span><\/p>\n

\u5bf9<\/span>FAO\u7684\u6291\u5236\u4f7f\u764c\u7ec6\u80de\u5bf9\u836f\u7269\u654f\u611f\uff0c\u5e76\u6709\u52a9\u4e8e\u514b\u670d\u80bf<\/span>\u7624\u8010\u836f\u3002\u8089\u6bd2\u78b1\u68d5\u6988\u9170\u8f6c\u79fb\u9176\uff08carnitine <\/span>palmitoyltransferase\uff0cCPT1\uff09\u5c06\u957f\u94fe\u8102\u80aa\u9178\u4ece\u80de\u8d28\u8fd0<\/span>\u8f93\u5230\u7ebf\u7c92\u4f53\u4e2d\u8fdb\u884c\u6c27\u5316\uff0c\u662f\u8102\u80aa\u9178\u6c27\u5316\u7684\u7b2c\u4e00\u4e2a\u9650<\/span>\u901f\u6b65\u9aa4\uff3b28\uff3d\u3002<\/span><\/p>\n

CPT1C \u8fc7\u8868\u8fbe\u7684\u80bf\u7624\u7ec6\u80de\u663e\u793aFAO \u589e<\/span>\u52a0\uff0cATP \u4ea7\u751f\u589e\u52a0\uff0c\u5e76\u4e14\u5bf9\u8461\u8404\u7cd6\u5265\u593a\u6216\u7f3a\u6c27\u5177\u6709\u6297<\/span>\u6027\u3002<\/span><\/p>\n

\u800c\u7f3a\u4e4fCPT1C \u7684\u764c\u7ec6\u80de\u4ea7\u751f\u8f83\u5c11\u7684ATP \u5e76\u4e14<\/span>\u5bf9\u4ee3\u8c22\u5e94\u6fc0\u66f4\u654f\u611f\u3002CPT1 \u5728\u8bb8\u591a\u80bf\u7624\u4e2d\u88ab\u8fc7\u5ea6\u8868<\/span>\u8fbe\uff0c\u5bf9\u764c\u7ec6\u80de\u7684\u5b58\u6d3b\u548c\u8010\u836f\u7684\u4ea7\u751f\u8d77\u7740\u81f3\u5173\u91cd\u8981\u7684<\/span>\u4f5c\u7528\u3002<\/span><\/p>\n

CPT1 \u6291\u5236\u5242Etomoxir \u53ef\u6539\u5584\u7d2b\u6749\u9187\u8010\u836f\u7ec6<\/span>\u80de\u5bf9\u7d2b\u6749\u9187\u7684\u654f\u611f\u6027\uff3b29\uff3d\u3002<\/span><\/p>\n

\u7528\u62c9\u8bfa\u62c9\u55ea\uff08ranolazine\uff09<\/span>\u6216siRNA \u9776\u5411CPT1 \u53ef\u4fc3\u8fdb\u4e86BCL-2 \u62ee\u6297\u5242ABT-<\/span>737 \u8bf1\u5bfc\u7684OCI-AML3 \u7ec6\u80de\u7684\u51cb\u4ea1\u3002<\/span>\u8102\u8d28\u5806\u79ef\u5728\u80bf\u7624\u4e2d\u975e\u5e38\u5e38\u89c1\uff0c\u5b83\u672c\u8eab\u53ef\u4ee5\u88ab\u89c6<\/span>\u4e3a\u764c\u75c7\u7684\u6807\u5fd7\u3002<\/span><\/p>\n

\u4f8b\u5982\uff0c\u4e73\u817a\u764c\u3001\u89c6\u7f51\u819c\u6bcd\u7ec6\u80de\u7624\u3001\u80ba<\/span>\u764c\u548c\u7ed3\u80a0\u764c\u4e2d\u8102\u80aa\u9178\u5408\u6210\u9176\uff08fatty acid synthase\uff0c<\/span>FASN\uff09\u548cATP \u67e0\u6aac\u9178\u88c2\u89e3\u9176\uff08ATP citrate lyase\uff0c<\/span>ACLY\uff09\u8868\u8fbe\u5f3a\u70c8\u589e\u52a0\uff0c\u8fdb\u4e00\u6b65\u4fc3\u8fdb\u80bf\u7624\u8fdb\u5c55\u548c\u8f6c<\/span>\u79fb\uff3b30\uff3d\u3002<\/span><\/p>\n

FASN\u548cACLY\u7684\u6291\u5236\u635f\u5bb3\u7ed3\u80a0\u764c\u7ec6\u80de\u7684\u8f6c<\/span>\u79fb\u548c\u8fdb\u5c55\uff0c\u5bfc\u81f4\u8f6f\u743c\u8102\u4e0a\u80bf\u7624\u7ec6\u80de\u8fc1\u79fb\u548c\u514b\u9686\u5f62\u6210<\/span>\u51cf\u5c11\uff3b31\uff3d\u3002<\/span><\/p>\n

\u8f6c\u5f55\u56e0\u5b50\uff0c\u753e\u9187\u8c03\u8282\u5143\u4ef6\u7ed3\u5408\u86cb\u767d1 \u548c<\/span>2\uff08SREBP1\/2\uff09\u662f\u8102\u80aa\u751f\u6210\u7a0b\u5e8f\u7684\u4e3b\u8981\u8f6c\u5f55\u8c03\u8282\u56e0<\/span>\u5b50\uff0c\u8bf1\u5bfc\u80c6\u56fa\u9187\u548c\u8102\u80aa\u9178\u7684\u751f\u7269\u5408\u6210\u3002<\/span><\/p>\n

\u540c\u65f6\uff0c<\/span>SREBPs \u4e5f\u662f\u80f6\u8d28\u6bcd\u7ec6\u80de\u7624\u9884\u540e\u4e0d\u826f\u7684\u6807\u5fd7\uff3b32\uff3d\u3002<\/span><\/p>\n

ACLY \u662f\u8102\u80aa\u4ece\u5934\u5408\u6210\u4e2d\u7684\u7b2c\u4e00\u79cd\u9650\u901f\u9176\uff0c\u901a<\/span>\u8fc7\u5c06\u67e0\u6aac\u9178\u76d0\u8f6c\u5316\u4e3a\u8349\u9170\u4e59\u9178\u548c\u4e59\u9170\u8f85\u9176A\uff0c\u8fdb\u800c<\/span>\u8f6c\u5316\u4e3a\u7ec6\u80de\u8d28\u4e2d\u7684\u8102\u80aa\u9178\uff0c\u4ece\u800c\u5c06\u8461\u8404\u7cd6\u4ee3\u8c22\u548c\u8102<\/span>\u80aa\u9178\u4ee3\u8c22\u8054\u7cfb\u8d77\u6765\u3002<\/span><\/p>\n

ACLY\u7684\u8868\u8fbe\u5728\u8bb8\u591a\u80bf\u7624\u4e2d\u4e0a<\/span>\u8c03\uff0c\u5305\u62ec\u7ed3\u76f4\u80a0\u764c\u3001\u80ba\u764c\u3001\u524d\u5217\u817a\u764c\u3001\u8180\u80f1\u764c\u3001\u809d\u764c\u3001<\/span>\u80c3\u764c\uff3b33\uff3d\u3002<\/span><\/p>\n

\u5728\u4eba\u80ba\u817a\u764c\u4e2d\uff0c\u78f7\u9178\u5316ACLY\u7684\u8868\u8fbe\u4e0e\u80bf<\/span>\u7624\u5206\u671f\u3001\u5206\u5316\u7a0b\u5ea6\u548c\u4e0d\u826f\u9884\u540e\u6709\u5173\uff3b33\uff3d\u3002<\/span><\/p>\n

\u6709\u6570\u636e\u8868\u660e<\/span>ACLY \u5728\u4f0a\u7acb\u66ff\u5eb7\u6d3b\u6027\u4ee3\u8c22\u4ea7\u7269SN38 \u8010\u836f\u4e2d\u8d77\u5173<\/span>\u952e\u4f5c\u7528\u3002<\/span><\/p>\n

\u901a\u8fc7\u6162\u75c5\u6bd2\u8f6c\u67d3\u7ed3\u80a0\u764c\u7ec6\u80deHT29 \u4f7f\u5176\u8fc7<\/span>\u8868\u8fbe\u5916\u6e90\u6027ACLY\uff0c\u5bfc\u81f4\u5176\u5bf9SN38 \u4ea7\u751f\u8010\u836f<\/span>\u6027\u3002\u800csiRNA \u6572\u4f4eACLY \u6216\u4f7f\u7528\u5c0f\u5206\u5b50\u6291\u5236\u5242<\/span>GSK165 \u6291\u5236ACLY \u6d3b\u6027\u4f7f\u7ed3\u76f4\u80a0\u764c\u7ec6\u80de\u5bf9<\/span>SN38 \u66f4\u654f\u611f\uff3b 34 \uff3d\u3002<\/span><\/p>\n

FASN \u4e5f\u662f\u8c03\u8282\u8102\u8d28\u4ece\u5934\u5408\u6210\u7684\u5173\u952e\u9176\uff0c\u53ef\u50ac\u5316\u78b3\u9aa8\u67b6\u7f29\u5408\u6210\u8102\u80aa\u9178\u3002<\/span><\/p>\n

\u5728\u524d\u5217\u817a\u764c\u7ec6\u80deLNCaP<\/span>\u4e2d\uff0cFASN\u8868\u8fbe\u4e0e\u7ec6\u80de\u51cb\u4ea1\u7387\u5448\u8d1f\u76f8\u5173\u3002FASN\u6c89\u9ed8<\/span>\u5bfc\u81f4\u7ec6\u80de\u51cb\u4ea1\uff0c\u800cFASN \u8fc7\u8868\u8fbe\u5219\u4fdd\u62a4\u524d\u5217\u817a\u4e0a\u76ae<\/span>\u7ec6\u80deiPrECs \u514d\u53d7\u559c\u6811\u78b1\u8bf1\u5bfc\u7684\u7ec6\u80de\u51cb\u4ea1\uff3b35\uff3d\u3002<\/p>\n

FASN\u6291\u5236\u5242\u5965\u5229\u53f8\u4ed6\uff08orlistat\uff09\u53ef\u4f7f\u767d\u8840\u75c5\u7ec6\u80deOCI –<\/span>AML3 \u5728\u5355\u72ec\u57f9\u517b\u6216\u4e0e\u9aa8\u9ad3\u6765\u6e90\u95f4\u5145\u8d28\u7ec6\u80de\u7684\u6ecb\u517b<\/span>\u5c42\u5171\u57f9\u517b\u65f6\u5bf9ABT-737 \u6cbb\u7597\u66f4\u654f\u611f\uff3b36\uff3d\u3002<\/span><\/p>\n

\u6709\u7814\u7a76\u63d0\u51fa\uff0c\u9798\u8102\u4ee3\u8c22\u7684\u6539\u53d8\u63d0\u9ad8\u4e86\u80bf\u7624\u7684\u5316<\/span>\u7597\u654f\u611f\u6027\uff0c\u4f7f\u5409\u897f\u4ed6\u6ee8\u8010\u836f\u7684\u80f0\u817a\u764c\u7ec6\u80de\u654f\u611f\uff3b37\uff3d\u3002<\/span><\/p>\n

\u4fc3\u751f\u5b58\u7684\u9798\u6c28\u9187-1-\u78f7\u9178\uff08S1P\uff09\u548c\u4fc3\u51cb\u4ea1\u7684\u795e\u7ecf\u9170\u80fa<\/span>\u9798\u8102\u5728\u7ec6\u80de\u5185\u7684\u5e73\u8861\u51b3\u5b9a\u4e86\u7ec6\u80de\u547d\u8fd0\uff0c\u7ec6\u80de\u795e\u7ecf\u9170<\/span>\u80fa\/S1P \u6bd4\u7387\u53ef\u9884\u6d4b\u80f0\u817a\u764c\u7ec6\u80de\u5bf9\u5409\u897f\u4ed6\u6ee8\u7684\u654f\u611f<\/span>\u6027\u3002<\/span><\/p>\n

\u4f7f\u7528\u9798\u6c28\u9187\u6fc0\u9176-1\uff08SphK1\uff09\u836f\u7406\u5b66\u6291\u5236\u5242\u6216\u795e<\/span>\u7ecf\u9170\u80fa\u7c7b\u4f3c\u7269\u4e0a\u8c03\u7ec6\u80de\u5185\u795e\u7ecf\u9170\u80fa\u6c34\u5e73\u6216\u4f7f\u7528<\/span>siRNA\u6765\u964d\u4f4eSphK1 \u6d3b\u6027\uff0c\u53ef\u589e\u52a0\u795e\u7ecf\u9170\u80fa\/ S1P \u6bd4<\/span>\u7387\uff0c\u4f7f\u80f0\u817a\u764c\u7ec6\u80de\u5bf9\u5409\u897f\u4ed6\u6ee8\u654f\u611f\u3002<\/span><\/p>\n

\u76f8\u53cd\uff0c\u901a\u8fc7\u4e0a<\/span>\u8c03SphK1 \u6d3b\u6027\u6765\u964d\u4f4e\u795e\u7ecf\u9170\u80fa\/S1P \u6bd4\u4f8b\uff0c\u4fc3\u8fdb\u4e86\u80f0<\/span>\u817a\u764c\u7ec6\u80de\u5bf9\u5409\u897f\u4ed6\u6ee8\u7684\u8010\u836f\u6027\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\n

1.5 \u5176\u4ed6<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\n
\n

\u6700\u8fd1\u7684\u6570\u636e\u8868\u660e\uff0c\u7ec6\u80de\u5185ATP \u662f\u5316\u7597\u8010\u836f\u6027\u7684\u5173\u952e\u56e0\u7d20\u3002\u7ec6\u80de\u5916ATP \u901a\u8fc7\u80de\u996e\u4f5c\u7528\u5185\u5316\uff0c\u8bf1\u5bfc<\/span>A549 \u7ec6\u80de\u5185ATP \u589e\u52a0\uff0c\u4e5f\u4f7f\u764c\u7ec6\u80de\u5bf9ATP \u7ade\u4e89\u7c7b\u6297<\/span>\u764c\u836f\u7269\u4ea7\u751f\u8010\u836f\u6027\uff3b38\uff3d\u3002<\/span><\/p>\n

\u6709\u7814\u7a76\u901a\u8fc7\u77ac\u65f6\u8f6c\u67d3\u6280\u672f<\/span>\u5c06\u5305\u88f9\u5728\u8102\u8d28\u4f53\u8f7d\u4f53\u4e2d\u7684ATP \u9012\u9001\u81f3HT29 \u548c<\/span>HCT116 \u7ec6\u80de\uff0c\u7ed3\u679c\u663e\u793a\uff0c\u5916\u6e90\u6027ATP \u8865\u5145\u90e8\u5206\u5730\u963b<\/span>\u65ad\u4e86\u5965\u6c99\u5229\u94c2\u5728\u4e24\u79cd\u7ec6\u80de\u7cfb\u4e2d\u7684\u7ec6\u80de\u6bd2\u6027\u4f5c\u7528\uff3b39\uff3d\u3002<\/span><\/p>\n

\u6700\u8fd1\u7684\u8bc1\u636e\u8868\u660e\uff0c\u7ebf\u7c92\u4f53\u7684\u7ec6\u80de\u95f4\u8f6c\u79fb\u5728\u80bf\u7624<\/span>\u5316\u7597\u8010\u836f\u4e2d\u4e5f\u53d1\u6325\u7740\u91cd\u8981\u4f5c\u7528\u3002\u4e0eMCF-7 \u5bf9\u7167\u76f8<\/span>\u6bd4\uff0c\u83b7\u5f97\u7684\u5185\u76ae\u7ec6\u80de\u7ebf\u7c92\u4f53\u7684MCF-7 \u663e\u793a\u51fa\u5bf9ADR<\/span>\u66f4\u9ad8\u7684\u5316\u5b66\u6297\u6027\uff081.91\uff5e3.19 \u500d\uff09\uff3b40\uff3d\u3002<\/span><\/p>\n

\u6025\u6027\u9ad3\u6027\u767d\u8840<\/span>\u75c5\uff08AML\uff09\u7ec6\u80de\u5728\u4e0e\u9aa8\u9ad3\u57fa\u8d28\u7ec6\u80de\u5171\u57f9\u517b\u7684\u8fc7\u7a0b\u4e2d<\/span>\u53ef\u4ee5\u4ece\u4e2d\u6444\u53d6\u7ebf\u7c92\u4f53\uff0c\u4f7f\u7ebf\u7c92\u4f53\u8d28\u91cf\u589e\u52a0\u9ad8\u8fbe<\/span>14%\u3002\u5728\u5316\u7597\u671f\u95f4\u5728\u4f53\u5185\u767d\u8840\u75c5\u8d77\u59cb\u7ec6\u80de\uff08LIC\uff09\u4e2d<\/span>\u89c2\u5bdf\u5230\u7c7b\u4f3c\u7684\u7ebf\u7c92\u4f53\u8f6c\u79fb\u3002<\/span><\/p>\n

LIC\u901a\u5e38\u4e0e\u5316\u5b66\u8010\u836f\u548c<\/span>\u75be\u75c5\u590d\u53d1\u76f8\u5173\uff3b41\uff3d\u3002<\/span><\/p>\n

\u8fd9\u4e9b\u7ed3\u679c\u8868\u660e\u80bf\u7624\u7ec6\u80de\u5177\u6709\u5f3a<\/span>\u5927\u7684\u4ee3\u8c22\u9002\u5e94\u6027\uff0c\u53ef\u514b\u670d\u4e0d\u5229\u73af\u5883\u8f6c\u53d8\u4e3a\u6709\u5229\u4e8e\u81ea<\/span>\u8eab\u751f\u957f\u7684\u4ee3\u8c22\u6a21\u5f0f\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n

\n
\n
\n
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\n

2<\/p>\n<\/section>\n<\/section>\n<\/section>\n

\n
\n

\u6297\u80bf\u7624\u836f\u8010\u836f\u540e\u80bf\u7624\u7ec4\u7ec7\u4e2d\u7684\u4ee3\u8c22\u91cd\u6392<\/strong><\/p>\n<\/section>\n<\/section>\n

<\/section>\n<\/section>\n<\/section>\n<\/section>\n
\n
\n
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2\uff0e1 \u7ec6\u80de\u6bd2\u7c7b\u836f\u7269\u8010\u836f\u540e\u7684\u80bf\u7624\u4ee3\u8c22\u6539\u53d8<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n

\n
\n
\n

ADR\u7684\u6297\u764c\u4f5c\u7528\u673a\u5236\u662f\u76f4\u63a5\u5d4c\u5165DNA\u6838\u78b1\u5bf9\u4e4b\u95f4\uff0c\u6216\u7834\u574f\u62d3\u6251\u5f02\u6784\u9176\uff0c\u4ece\u800c\u5e72\u6270\u6838\u9178\u7684\u5408\u6210\u3002<\/span><\/p>\n

ADR\u8bf1\u5bfcMCF-7 \u7ec6\u80de\u8010\u836f\u7684\u8fc7\u7a0b\u4e2d\uff0c\u86cb\u767d\u8d28\u3001\u560c\u5464\u3001\u5627\u5576\u548c\u8c37\u80f1\u7518\u80bd\u751f\u7269\u5408\u6210\u88ab\u663e\u8457\u6291\u5236\uff0c\u7518\u6cb9\u4ee3\u8c22\u548c<\/span>\u7cd6\u9175\u89e3\u589e\u5f3a\u3002<\/span><\/p>\n

ADR \u957f\u671f\u7ed9\u836f\u8fd8\u6291\u5236\u4e86MCF-7 \u7ec6\u80de<\/span>\u4e2d\u80f1\u6c28\u9178\uff08GSH\u7684\u6765\u6e90\uff09\u7684\u6d41\u5165\u548cSLC7A11 \u8f6c\u8fd0\u86cb<\/span>\u767d\uff08\u8d1f\u8d23\u80f1\u6c28\u9178\u6444\u53d6\uff09\u7684\u6d3b\u6027\u3002SLC7A11 \u7684\u4e0b\u8c03\/\u6c89<\/span>\u9ed8\uff0c\u6216\u80f1\u6c28\u9178\u7684\u7f3a\u5931\uff0c\u6216ROS \u66b4\u9732\u7684\u589e\u5f3a\uff0c\u5747\u663e\u8457\u589e<\/span>\u52a0\u4e86MCF-7 \u7ec6\u80de\u4e2dP-gp \u7684\u8868\u8fbe\u548c\u8010\u836f\u6027\uff3b42\uff3d\u3002<\/p>\n

\u987a\u94c2\u4e3b\u8981\u901a\u8fc7\u4ea7\u751f\u6838\u4ec1DNA \u52a0\u5408\u7269\uff0c\u4f7fDNA<\/span>\u7ed3\u6784\u53d8\u5f62\uff0c\u4f7f\u5176\u590d\u5236\u8f6c\u5f55\u51fa\u73b0\u969c\u788d\uff0c\u8fdb\u800c\u53d1\u6325\u6297\u764c<\/span>\u4f5c\u7528\u7684\u3002\u987a\u94c2\u53ef\u4ee5\u589e\u52a0\u5375\u5de2\u764c\u7ec6\u80deA2780 \u4e2dHIF-<\/span>1\u03b1 \u7684\u964d\u89e3\uff0c\u4f46\u6ca1\u6709\u4e0b\u8c03\u8010\u836f\u7ec6\u80deA2780\/CP \u4e2d\u7684<\/span>HIF-1\u03b1\u3002<\/span><\/p>\n

\u6572\u4f4eA2780\/CP \u7ec6\u80de\u4e2d\u7684HIF-1\u03b1 \u4fc3\u4f7f\u7ec6\u80de<\/span>\u4ee3\u8c22\u4ece\u7cd6\u9175\u89e3\u504f\u5411\u7ebf\u7c92\u4f53OXPHOS\uff0c\u4ea7\u751f\u8fc7\u91cfROS<\/span>\u5bfc\u81f4\u7ec6\u80de\u6b7b\u4ea1\uff0c\u6539\u5584\u4e86A2780\/CP \u7ec6\u80de\u5bf9\u987a\u94c2\u7684<\/span>\u53cd\u5e94\uff3b43\uff3d\u3002<\/span><\/p>\n

\u7528Ara-C\u6cbb\u7597\u60a3\u8005\u6765\u6e90\u7684\u6025\u6027\u9ad3\u7cfb\u767d\u8840\u75c5\u5f02\u79cd\u79fb\u690d\u7624\u540e\uff0cAra-C\u8010\u836f\u7ec6\u80de\u663e\u793a\u51fa\u9ad8\u6c34\u5e73\u7684\u6d3b\u6027\u6c27\uff0c<\/span>\u7ebf\u7c92\u4f53\u8d28\u91cf\u589e\u52a0\uff0c\u5e76\u4fdd\u7559\u6d3b\u6027\u6781\u5316\u7ebf\u7c92\u4f53\uff0c\u4e0e\u9ad8<\/span>OXPHOS \u72b6\u6001\u4e00\u81f4\u3002<\/span><\/p>\n

\u8fd9\u8868\u660eAra-C\u5316\u7597\u8bf1\u5bfc\u80bf\u7624\u4ee3<\/span>\u8c22\u4ece\u7cd6\u9175\u89e3\u5411OXPHOS \u8f6c\u53d8\u3002\u6b64\u5916\uff0cAra-C \u6cbb\u7597\u540e<\/span>\u6b8b\u7559\u7684\u7ec6\u80de\u8fd8\u8868\u73b0\u51fa\u8102\u80aa\u9178\u6c27\u5316\u589e\u52a0\uff0cCD36 \u8868\u8fbe<\/span>\u4e0a\u8c03\u7684\u7279\u5f81\u3002<\/span><\/p>\n

\u53e6\u5916\uff0c\u5728\u5229\u7528\u534a\u4e73\u7cd6\u4f5c\u4e3a\u552f\u4e00\u78b3\u6e90\u8bf1<\/span>\u5bfc\u4eba\u767d\u8840\u75c5U937 \u7ec6\u80de\u8f6c\u5411\u9ad8OXPHOS \u4ee3\u8c22\u540e\uff0c\u660e<\/span>\u663e\u6291\u5236\u4e86Ara-C\u5bfc\u81f4\u7684\u7ec6\u80de\u51cb\u4ea1\u3002<\/span><\/p>\n

\u66ff\u52a0\u73af\u7d20\u5904\u7406\u7684<\/span>\u9ad8OXPHOS \u7684MOLM14 \u7ec6\u80de\u663e\u793a\u51fa\u7535\u5b50\u4f20\u9012\u94fe\u590d<\/span>\u5408\u7269\u86cb\u767d\u8d28\u7ffb\u8bd1\u6c34\u5e73\u663e\u8457\u964d\u4f4e\uff0c\u7ebf\u7c92\u4f53\u8d28\u91cf\u548c\u819c\u7535<\/span>\u4f4d\u4ee5\u53ca\u6c27\u8017\u7387\u964d\u4f4e\uff0c\u5e76\u663e\u8457\u589e\u5f3aAra-C \u7684\u6297\u767d\u8840\u75c5<\/span>\u4f5c\u7528\uff3b44\uff3d\u3002<\/span><\/p>\n

\u7d2b\u6749\u9187\u7c7b\u836f\u7269\u901a\u8fc7\u7a33\u5b9a\u5fae\u7ba1\u86cb\u767d\u963b\u788d\u7ec6\u80de\u5468\u671f\u7684\u8fdb\u7a0b\u3002MDA-MB-435 \u7d2b\u6749\u9187\u8010\u836f\u7684\u4e73\u817a\u764c\u7ec6<\/span>\u80de\u4e2d\uff0cLDHA\u7684\u8868\u8fbe\u548c\u6d3b\u6027\u663e\u8457\u9ad8\u4e8e\u7d2b\u6749\u9187\u654f\u611f\u7ec6<\/span>\u80de\u3002\u5e94\u7528siRNA\u4e0b\u8c03LDH-A\u663e\u8457\u589e\u52a0\u4e86\u7d2b\u6749\u9187\u8010<\/span>\u836f\u7ec6\u80de\u5bf9\u7d2b\u6749\u9187\u7684\u654f\u611f\u6027\u3002\u800c\u4e14\uff0c\u7d2b\u6749\u9187\u8010\u836f\u7ec6\u80de<\/span>\u663e\u793a\u5bf9\u7279\u5f02\u6027LDH \u6291\u5236\u5242\u8349\u6c28\u9178\u6709\u66f4\u9ad8\u654f\u611f\u6027\u3002<\/span><\/p>\n

\u8fd9\u4e9b\u7ed3\u679c\u8868\u660e\u4e73\u9178\u4ee3\u8c22\u5728\u7d2b\u6749\u9187\u7684\u8010\u836f\u8fc7\u7a0b\u4e2d\u5177\u6709\u91cd\u8981\u4f5c\u7528\u3002<\/span><\/p>\n

5-FU \u8010\u836f\u7684\u7ed3\u80a0\u764c\u7ec6\u80de\u4e0e\u654f\u611f\u7ed3\u80a0\u764c\u7ec6\u80de\u76f8\u6bd4\uff0cmiR-122 \u663e\u8457\u4e0b\u8c03\uff0cGlut-1\u3001PKM2 \u548cLDHA\u663e\u8457<\/span>\u4e0a\u8c03\u3002<\/span><\/p>\n

\u5355\u72ec\u8f6c\u67d3miR-122 \u8fdb\u5165HCT116 \u7ec6\u80de\u964d\u4f4e\u4e86<\/span>PKM2 \u7684\u8868\u8fbe\u5e76\u4f7f\u8010\u836fHCT116 \u5bf95-FU\u654f\u611f\u3002\u540c\u65f6<\/span>\u8f6c\u67d3miR-122 \u548cPKM2 \u4f7fHCT116 \u8010\u836f\u3002\u8fd9\u4e9b\u7ed3\u679c<\/span>\u8868\u660ePKM2 \u5728\u8c03\u8282HCT116 \u5bf95-FU \u654f\u611f\u6027\u4e2d\u53d1\u6325<\/span>\u4e86\u91cd\u8981\u4f5c\u7528\u3002<\/span><\/p>\n

\u901a\u8fc7\u76f4\u63a5\u6291\u5236PKM2 \u7684\u8868\u8fbe\u53ef\u4ee5\u514b\u670d<\/span>\u7ed3\u80a0\u764c\u7ec6\u80de\u5bf95-FU\u7684\u6297\u6027\uff3b45\uff3d\u3002<\/span><\/p>\n

\u8010\u836f\u7ed3\u80a0\u764c\u7ec6\u80de\u5206<\/span>\u6ccc\u7684\u86cb\u767d\u4e2d\uff0c\u7cd6\u9175\u89e3\u9176\u5305\u62ec\u7518\u6cb9\u919b-3-\u78f7\u9178\u8131\u6c22\u9176\u3001<\/span>PKM2\u3001\u8f6c\u916e\u9176\u548c\u82f9\u679c\u9178\u91761 \u5747\u663e\u8457\u589e\u52a0\u3002<\/span><\/p>\n

\u5bf946 \u4f8b<\/span>\u7ed3\u80a0\u764c\u60a3\u8005\u7684\u8840\u6e05\u8fdb\u884c\u5206\u6790\uff0c\u7ed3\u679c\u663e\u793a5-FU\u6cbb\u7597\u65e0<\/span>\u54cd\u5e94\u7684\u60a3\u8005\u8840\u6e05\u4e2dPKM2 \u660e\u663e\u589e\u52a0\uff3b46\uff3d\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\n

2. 2 \u916a\u6c28\u9178\u6fc0\u9176\u6291\u5236\u5242\u8010\u836f\u540e\u7684\u80bf\u7624\u4ee3\u8c22\u6539\u53d8<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n

\n
\n
\n

\u6297\u80bf\u7624\u8840\u7ba1\u751f\u6210\u7684\u916a\u6c28\u9178\u6fc0\u9176\u6291\u5236\u5242\u6cbb\u7597\u5177\u6709\u9ad8\u6548\u3001\u6291\u7624\u8c31\u5e7f\u3001\u65e0\u660e\u663e\u6bd2\u526f\u4f5c\u7528\u7b49\u4f18\u52bf\uff0c\u4f46\u957f\u671f<\/span>\u4f7f\u7528\u4ecd\u65e7\u4f1a\u51fa\u73b0\u8010\u836f\uff0c\u5bfc\u81f4\u80bf\u7624\u590d\u53d1\u6216\u8f6c\u79fb\u3002<\/span><\/p>\n

\u6297\u80bf<\/span>\u7624\u8840\u7ba1\u751f\u6210\u836f\u7269\u5f15\u8d77\u7684\u80bf\u7624\u4ee3\u8c22\u53d8\u5316\u4e3b\u8981\u5f52\u56e0\u4e8e<\/span>\u7f3a\u6c27\u548c\u8425\u517b\u4f9b\u5e94\u4e0d\u8db3\u3002<\/span><\/p>\n

Sounni \u7b49\uff3b47\uff3d\u7814\u7a76\u4e86sunitinib<\/span>\u8bf1\u5bfc\u7684\u80bf\u7624\u4ee3\u8c22\u7d0a\u4e71\u3002\u901a\u8fc7\u86cb\u767d\u8d28\u7ec4\u5b66\u548c\u8f6c\u5f55\u7ec4<\/span>\u5b66\u5206\u6790\u53d1\u73b0\uff0c\u64a4\u836f\u540e\u8102\u80aa\u9178\u3001\u4e19\u916e\u9178\u548c\u6c28\u57fa\u9178\u4ee3\u8c22<\/span>\u589e\u52a0\uff0c\u800c\u8461\u8404\u7cd6\u4ee3\u8c22\u548c\u78b3\u6c34\u5316\u5408\u7269\u5206\u89e3\u4ee3\u8c22\u5219\u4e0b<\/span>\u8c03\u3002<\/span><\/p>\n

\u8fd9\u79cd\u4ee3\u8c22\u53d8\u5316\u4e0e\u80bf\u7624\u5feb\u901f\u751f\u957f\u548c\u4fb5\u88ad\u6027\u589e\u52a0<\/span>\u76f8\u5173\u3002<\/span><\/p>\n

\u5728\u8102\u80aa\u7ec4\u7ec7\u9644\u8fd1\u751f\u957f\u7684\u764c\u75c7\uff0c\u5305\u62ec\u4e73\u817a\u764c\u3001<\/span>\u524d\u5217\u817a\u764c\u3001\u80f0\u817a\u764c\u548c\u809d\u7ec6\u80de\u764c\uff0c\u5f80\u5f80\u8868\u73b0\u51fa\u5bf9\u6297\u8840<\/span>\u7ba1\u751f\u6210\u6cbb\u7597\u7684\u8010\u836f\u6027\uff0c\u800c\u963b\u65ad\u8102\u8d28\u5408\u6210\u53ef\u4ee5\u6291\u5236\u80bf<\/span>\u7624\u8f6c\u79fb\u53ca\u8010\u836f\u6027\u7684\u4ea7\u751f\u3002<\/span><\/p>\n

\u5728sunitinib \u6216sorafenib \u6cbb<\/span>\u7597\u671f\u95f4\uff0c\u80bf\u7624\u751f\u957f\u53d7\u5230\u6291\u5236\u3002\u7136\u800c\uff0c\u6cbb\u7597\u505c\u6b62\u540e\u8840<\/span>\u7ba1\u6062\u590d\u751f\u957f\uff0c\u8102\u8d28\u5408\u6210\u589e\u5f3a\uff0c\u80bf\u7624\u518d\u751f\u3002\u4f7f\u7528orlistat<\/span>\u6216shRNA \u4e0b\u8c03FASN\uff0c\u6291\u5236\u4e86sunitinib \u6cbb\u7597\u6212\u65ad\u540e<\/span>\u7684\u80bf\u7624\u518d\u751f\u548c\u8f6c\u79fb\u3002<\/span><\/p>\n

\u6700\u8fd1\u7684\u51e0\u9879\u4f53\u5185\u5b9e\u9a8c\u8868\u660e\uff0c\u7cd6<\/span>\u9175\u89e3\u7684\u8d1f\u8c03\u8282\u53ef\u80fd\u589e\u5f3a\u80bf\u7624\u6297VEGF \u6cbb\u7597\u7684\u6cbb\u7597\u6548<\/span>\u529b\uff3b48\uff3d\u3002<\/span><\/p>\n

\u8fd9\u53ef\u80fd\u662f\u56e0\u4e3a\u6297VEGF \u6cbb\u7597\u540e\uff0c\u9ad8\u7cd6\u9175\u89e3\u7684<\/span>\u80bf\u7624\u7ec6\u80de\u8868\u578b\u5f97\u5230\u52a0\u5f3a\u3002\u5728\u809d\u7ec6\u80de\u764c\u6a21\u578b\u4e2d\u5229\u7528<\/span>\u4e8c\u6c2f\u4e59\u9178\uff08DCA\uff09\u8c03\u8282\u7cd6\u9175\u89e3\u53ef\u4ee5\u63d0\u9ad8sorafenib \u7684<\/span>\u6cbb\u7597\u6548\u529b\uff3b49\uff3d\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n

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2\uff0e3 \u6297\u4f53\u7c7b\u836f\u7269\u8010\u836f\u540e\u7684\u80bf\u7624\u4ee3\u8c22\u53d8\u5316<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u7814\u7a76\u53d1\u73b0\u4e73\u817a\u764c\u7ec6\u80de\u5bf9\u9776\u5411HER-2 \u7684\u66f2\u59a5\u73e0\u5355\u6297\u7684\u8010\u836f\u6027\u4e0e\u7cd6\u9175\u89e3\u901a\u8def\u589e\u5f3a\u76f8\u5173\u3002<\/span><\/p>\n

\u8010\u836f\u80bf\u7624<\/span>\u5448\u73b0\u70ed\u4f11\u514b\u56e0\u5b501 \u548cLDHA\u4e0a\u8c03\u7684\u8d8b\u52bf\u3002\u66f2\u59a5\u73e0\u5355<\/span>\u6297\u4e0e\u7cd6\u9175\u89e3\u6291\u5236\u5242\u8054\u5408\u5e94\u7528\u53ef\u5728\u4f53\u5916\u548c\u4f53\u5185\u534f\u540c<\/span>\u6291\u5236\u66f2\u59a5\u73e0\u5355\u6297\u8010\u836f\u7684\u4e73\u817a\u764c\uff3b50\uff3d\u3002<\/span><\/p>\n

Bensaad \u7b49\uff3b51\uff3d\u89c2<\/span>\u5bdf\u5230\uff0c\u9776\u5411VEGF \u7684\u8d1d\u4f10\u5355\u6297\u6cbb\u7597\u540e\u7684\u80bf\u7624\u4e2d\u8102\u80aa<\/span>\u9178\u6444\u53d6\u548c\u4ee3\u8c22\u4e0a\u8c03\uff0c\u5305\u62ecFATBP3 \u548cFATBP7 \u7684\u8868\u8fbe<\/span>\u589e\u52a0\uff0c\u4ee5\u53ca\u80bf\u7624\u7ec6\u80de\u4e2d\u8102\u6ef4\u7684\u79ef\u7d2f\u3002<\/span><\/p>\n

Keunen \u7b49\uff3b52\uff3d\u901a<\/span>\u8fc7\u78c1\u5171\u632f\u6ce2\u8c31\u5b66\u7814\u7a76\u4e86\u7528\u8d1d\u4f10\u5355\u6297\u6cbb\u7597\u7684\u80f6\u8d28\u6bcd<\/span>\u7ec6\u80de\u7624\u5f02\u79cd\u79fb\u690d\u7269\u7684\u4ee3\u8c22\u7279\u5f81\uff0c\u53d1\u73b0\u4e73\u9178\u548c\u4e19\u6c28\u9178<\/span>\u4ee3\u8c22\u7269\u7684\u589e\u52a0\uff0c\u4ee5\u53caHIF-1\u03b1 \u548cPI3K \/ AKT \u9014\u5f84\u6fc0<\/span>\u6d3b\u3002<\/span><\/p>\n

\u901a\u8fc7\u900f\u5c04\u7535\u5b50\u663e\u5fae\u955c\u89c2\u5bdf\u53d1\u73b0\uff0c\u7528\u8d1d\u4f10\u5355\u6297\u6cbb<\/span>\u7597\u7684GBM\u5f02\u79cd\u79fb\u690d\u7269\u4e2d\u7ebf\u7c92\u4f53\u6570\u91cf\u663e\u8457\u51cf\u5c11\u3002<\/span><\/p>\n

\u6b64<\/span>\u5916\uff0c\u5c06\u7ed3\u80a0\u764c\u7ec6\u80de\u63a5\u79cd\u5230\u8102\u80aa\u53d8\u6027\u6216\u6b63\u5e38\u809d\u810f\u7ec4\u7ec7<\/span>\u4e2d\uff0c\u53d1\u73b0\u690d\u5165\u8102\u80aa\u809d\u7ec4\u7ec7\u7684\u80bf\u7624\u8840\u7ba1\u5bc6\u5ea6\u8f83\u9ad8\u3002<\/span><\/p>\n

\u6297<\/span>\u8840\u7ba1\u751f\u6210\u836f\u7269\u8d1d\u4f10\u5355\u6297\u6cbb\u7597\u540e\u8bf1\u5bfc\u80bf\u7624\u7f3a\u6c27\uff0c\u542f\u52a8<\/span>FAO \u4ee3\u8c22\u91cd\u7f16\u7a0b\uff0c\u589e\u52a0\u6e38\u79bb\u8102\u80aa\u9178\u7684\u6444\u53d6\uff0c\u523a\u6fc0\u80bf<\/span>\u7624\u7ec6\u80de\u518d\u589e\u6b96\u3002<\/span><\/p>\n

\u800cEtomoxir \u5bf9CPT1 \u7684\u6291\u5236\u663e\u8457\u6291<\/span>\u5236\u6e38\u79bb\u8102\u80aa\u9178\u8bf1\u5bfc\u7684\u7ec6\u80de\u589e\u6b96\uff0c\u4f7f\u8d1d\u4f10\u5355\u6297\u5bf9\u8102\u80aa<\/span>\u809d\u4e2d\u751f\u957f\u7684\u80bf\u7624\u7597\u6548\u589e\u5f3a\uff3b53\uff3d\u3002<\/span><\/p>\n<\/section>\n<\/section>\n<\/section>\n

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<\/section>\n<\/section>\n<\/section>\n<\/section>\n
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\u76ee\u524d\u9488\u5bf9\u80bf\u7624\u4ee3\u8c22\u7684\u836f\u7269<\/span>\u7814\u53d1\u4e3b\u8981\u4ee5\u80bf\u7624\u7ec6\u80de\u4ee3\u8c22\u901a\u8def\u4e2d\u7279\u5f81\u53d8\u5316\u7684\u8f6c\u8fd0<\/span>\u4f53\u53ca\u5173\u952e\u9176\u4f5c\u4e3a\u9776\u70b9\uff0c\u5176\u4e2d\u4ee5\u8461\u8404\u7cd6\u4ee3\u8c22\u7814\u7a76\u5f97\u6700<\/span>\u4e3a\u5e7f\u6cdb\u548c\u6df1\u5165\uff0c\u5df2\u6709\u591a\u4e2a\u836f\u7269\u8fdb\u5165\u4e34\u5e8a\u7814\u7a76\u9636\u6bb5\uff0c\u5305\u62ec2-DG\uff08\u9776\u5411HK2\uff09\u3001DCA\uff08\u9776\u5411PDK\uff09\u30013PO\uff08\u9776<\/span>\u5411PFK2\uff09\u3001AZD3965\uff08\u9776\u5411MCT1\uff09\u7b49\u3002<\/span><\/p>\n

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\"\u80bf\u7624\u4ee3\u8c22\u91cd\u6392\u4e0e\u80bf\u7624\u8010\u836f\u76f8\u5173\u6027\u7684\u7814\u7a76\u8fdb\u5c55\"<\/p>\n
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\u53c2\u8003\u6587\u732e<\/strong><\/p>\n<\/section>\n

\"\u80bf\u7624\u4ee3\u8c22\u91cd\u6392\u4e0e\u80bf\u7624\u8010\u836f\u76f8\u5173\u6027\u7684\u7814\u7a76\u8fdb\u5c55\"<\/section>\n

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\u8be6\u89c1\u539f\u6587\uff0c\u836f\u7269\u8bc4\u4ef7\u7814\u7a76\uff0cDrug Evaluation Research <\/span><\/p>\n

\u7b2c42 \u5377\u7b2c3 \u671f<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u514d\u8d23\u58f0\u660e<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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\u672c\u516c\u4f17\u53f7\u53d1\u5e03\u7684\u6587\u7ae0\u5747\u4e3a\u4fc3\u8fdb\u5236\u836f\u754c\u540c\u884c\u7684\u4ea4\u6d41\u4e0e\u5b66\u4e60\uff1b\u5185\u5bb9\u7531\u5c0f\u7f16\u67e5\u9605\u6587\u732e\u9009\u53d6\uff0c\u6392\u7248\u4e0e\u7f16\u8f91\u4e3a\u539f\u521b\u3002\u5982\u8f6c\u8f7d\uff0c\u8bf7\u6ce8\u660e\u6765\u6e90\u3002<\/p>\n

\u9009\u53d6\u7684\u6587\u7ae0\u5df2\u660e\u786e\u6ce8\u660e\u6765\u6e90\u548c\u4f5c\u8005\uff0c\u7248\u6743\u5f52\u539f\u4f5c\u8005\u6240\u6709\uff0c\u5982\u6d89\u53ca\u4f5c\u54c1\u5185\u5bb9\u3001\u7248\u6743\u6216\u5176\u4ed6\u95ee\u9898\uff0c\u8bf7\u8054\u7cfb\u6211\u4eec\u8fdb\u884c\u5220\u9664\u3002<\/strong><\/p>\n

\u6587\u7ae0\u5185\u5bb9\u4e3a\u4f5c\u8005\u89c2\u70b9\uff0c\u4e0d\u4ee3\u8868\u672c\u516c\u4f17\u53f7\u7acb\u573a\u3002<\/p>\n<\/section>\n<\/section>\n<\/section>\n

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\u6587\u7ae0\u641c\u7d22<\/strong><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n

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\u672c\u516c\u4f17\u53f7\u5f00\u901a\u5f80\u671f\u6587\u7ae0\u4efb\u610f\u641c\u529f\u80fd\u5566
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\"\u80bf\u7624\u4ee3\u8c22\u91cd\u6392\u4e0e\u80bf\u7624\u8010\u836f\u76f8\u5173\u6027\u7684\u7814\u7a76\u8fdb\u5c55\"<\/section>\n<\/section>\n<\/section>\n<\/section>\n


<\/p>\n<\/p><\/div>\n","protected":false},"excerpt":{"rendered":"

\u80bf\u7624\u4ee3\u8c22\u91cd\u6392\u4e0e\u80bf\u7624\u8010\u836f\u76f8\u5173\u6027\u7684\u7814\u7a76\u8fdb\u5c55 Advances in studies on relationship […]<\/p>\n","protected":false},"author":1,"featured_media":98775,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/98774"}],"collection":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/comments?post=98774"}],"version-history":[{"count":1,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/98774\/revisions"}],"predecessor-version":[{"id":98785,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/98774\/revisions\/98785"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media\/98775"}],"wp:attachment":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media?parent=98774"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/categories?post=98774"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/tags?post=98774"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}