{"id":9998,"date":"2018-06-12T01:00:00","date_gmt":"2018-07-27T09:43:31","guid":{"rendered":"http:\/\/www.05921.net\/index.php\/2018\/07\/27\/%e4%b8%ad%e5%9b%bd%e5%88%86%e5%ad%90%e8%af%8a%e6%96%ad%e4%ba%a7%e4%b8%9a%e6%88%98%e7%95%a5%e7%a0%94%e7%a9%b6\/"},"modified":"2018-07-27T17:43:32","modified_gmt":"2018-07-27T09:43:32","slug":"%e4%b8%ad%e5%9b%bd%e5%88%86%e5%ad%90%e8%af%8a%e6%96%ad%e4%ba%a7%e4%b8%9a%e6%88%98%e7%95%a5%e7%a0%94%e7%a9%b6","status":"publish","type":"post","link":"https:\/\/www.05921.net\/index.php\/9998\/","title":{"rendered":"\u4e2d\u56fd\u5206\u5b50\u8bca\u65ad\u4ea7\u4e1a\u6218\u7565\u7814\u7a76"},"content":{"rendered":"<div class=\"bpp-post-content\">\n<p><img class=\"\"       style=\"\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/07\/beepress9-1532684612.jpeg\" alt=\"\u4e2d\u56fd\u5206\u5b50\u8bca\u65ad\u4ea7\u4e1a\u6218\u7565\u7814\u7a76\" title=\"\u4e2d\u56fd\u5206\u5b50\u8bca\u65ad\u4ea7\u4e1a\u6218\u7565\u7814\u7a76\"  \/><\/p>\n<blockquote style=\"max-width: 100%;color: rgb(51, 51, 51);\">\n<section class=\"\"     style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section class=\"\"  style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<p style=\"max-width: 100%;min-height: 1em;line-height: 1.75em;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word 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class=\"\"     style=\"max-width: 100%;color: rgb(51, 51, 51);\">\n<section style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section class=\"\"  style=\"max-width: 100%;box-sizing: border-box;text-align: center;word-wrap: break-word !important;\">\n<p style=\"margin-top: 10px;margin-bottom: 10px;max-width: 100%;box-sizing: border-box;min-height: 1em;line-height: 1.75em;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<section class=\"\" style=\"max-width: 100%;box-sizing: border-box;display: inline-block;vertical-align: top;word-wrap: break-word !important;\">\n<section style=\"margin-right: -1px;margin-left: auto;max-width: 100%;box-sizing: border-box;width: 12px;height: 12px;border-top: 3px solid rgb(255, 202, 0);border-right: 3px solid rgb(255, 202, 0);transform: rotate(0deg);word-wrap: break-word !important;\"><\/section>\n<section style=\"margin-top: 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!important;\"><\/section>\n<\/section>\n<p style=\"max-width: 100%;min-height: 1em;box-sizing: border-box !important;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;color: rgb(255, 169, 0);font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\uff08\u4e00\uff09\u5206\u5b50\u8bca\u65ad\u6280\u672f\u4ecb\u7ecd<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word 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style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word 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style=\"max-width: 100%;box-sizing: border-box;color: rgb(51, 51, 51);\">\n<section class=\"\"  style=\"max-width: 100%;box-sizing: border-box;text-align: center;word-wrap: break-word !important;\">\n<section class=\"\" style=\"max-width: 100%;box-sizing: border-box;display: inline-block;vertical-align: top;word-wrap: break-word !important;\">\n<section style=\"margin-right: -1px;margin-left: auto;max-width: 100%;box-sizing: border-box;width: 12px;height: 12px;border-top: 3px solid rgb(255, 202, 0);border-right: 3px solid rgb(255, 202, 0);transform: rotate(0deg);word-wrap: break-word !important;\"><\/section>\n<section style=\"margin-top: -11px;margin-bottom: -11px;padding: 4px;max-width: 100%;box-sizing: border-box;border-width: 1px;border-style: solid;border-color: rgb(223, 223, 223);word-wrap: break-word !important;\">\n<section class=\"\" style=\"padding-right: 6px;padding-left: 6px;max-width: 100%;box-sizing: border-box;border-width: 1px;border-style: solid;border-color: rgb(223, 223, 223);font-size: 14px;word-wrap: break-word !important;\">\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\u4e8c\u3001\u5206\u5b50\u8bca\u65ad\u4ea7\u4e1a\u56fd\u5185\u5916\u7814\u7a76\u73b0\u72b6<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"margin-left: -1px;max-width: 100%;box-sizing: border-box;width: 12px;height: 12px;border-bottom: 3px solid rgb(255, 202, 0);border-left: 3px solid rgb(255, 202, 0);transform: rotate(0deg);word-wrap: break-word !important;\"><\/section>\n<\/section>\n<p style=\"max-width: 100%;min-height: 1em;box-sizing: border-box !important;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<\/section>\n<\/section>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 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!important;\">\u4f53\u5916\u8bca\u65ad\uff08IVD\uff09\u662f\u6307\u8fd0\u7528\u533b\u7597\u4eea\u5668\u548c\u8bd5\u5242\u7b49\u6765\u68c0\u6d4b\u4eba\u4f53\u7684\u8840\u6db2\u3001\u5c3f\u6db2\u3001\u7caa\u4fbf\u3001\u7ec4\u7ec7\u548c\u5176\u4ed6\u4f53\u6db2\u7b49\u6807\u672c\uff0c\u4ee5\u8fbe\u5230\u68c0\u6d4b\u75be\u75c5\u3001\u611f\u67d3\u7b49\u60c5\u51b5\u7684\u76ee\u7684\u3002\u7f8e\u56fd\u8054\u5408\u5e02\u573a\u7814\u7a76\uff08AMR\uff09\u673a\u6784\u5173\u4e8e\u4f53\u5916\u8bca\u65ad\u7684\u62a5\u544a\u6307\u51fa\uff0c\u5168\u7403\u4f53\u5916\u8bca\u65ad\u5e02\u573a\u57282013\u5e74\u7684\u4ef7\u503c\u4e3a533\u4ebf\u7f8e\u5143\uff0c\u9884\u8ba12018\u5e74\u53ef\u8fbe\u5230588\u4ebf\u7f8e\u5143\uff0c2020\u5e74\u53ef\u8fbe\u5230747\u4ebf\u7f8e\u5143[2]\u3002\u7f8e\u56fd\u662f\u5168\u7403\u4f53\u5916\u8bca\u65ad\u7684\u521b\u65b0\u4e2d\u5fc3\u548c\u6700\u5927\u7684\u9700\u6c42\u5e02\u573a\uff0c\u6bcf\u5e74\u4ecd\u4fdd\u63013%~5%\u7684\u5e74\u5ea6\u590d\u5408\u589e\u957f\u7387\uff08CAGR\uff09\uff0c2020\u5e74\u5c06\u8fbe\u5230301\u4ebf\u7f8e\u5143\u3002\u4e9a\u6d32\u5219\u5177\u6709\u6700\u5feb\u7684\u589e\u957f\u901f\u5ea6\uff0cCAGR\u53ef\u8fbe7.49%[2]\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u5728\u4f53\u5916\u8bca\u65ad\u884c\u4e1a\u4f17\u591a\u7684\u7ec6\u5206\u5e02\u573a\u4e2d\uff0c\u5206\u5b50\u8bca\u65ad\u7684\u8868\u73b0\u6700\u4e3a\u7a81\u51fa\uff0c\u88ab\u8ba4\u4e3a\u662f\u4f53\u5916\u8bca\u65ad\u884c\u4e1a\u7684\u4e3b\u8981\u589e\u957f\u52a8\u529b\u3002\u5206\u5b50\u8bca\u65ad\u5360\u4f53\u5916\u8bca\u65ad\u7684\u6bd4\u4f8b\u5df2\u7ecf\u4ece1995\u5e74\u76842%\u589e\u957f\u5230\u4e862012\u5e74\u768411%\u30021995\u5e74\uff0c\u5168\u7403\u4ece\u4e8b\u5206\u5b50\u8bca\u65ad\u4ea7\u54c1\u7684\u516c\u53f8\u4ec5\u6709100\u5bb6\uff0c\u968f\u7740\u5206\u5b50\u8bca\u65ad\u5e02\u573a\u7684\u8fc5\u901f\u53d1\u5c55\uff0c\u8fd9\u4e00\u6570\u5b57\u5df2\u589e\u52a0\u81f32010\u5e74\u7684500\u591a\u5bb6[3]\u3002\u8f83\u4e3a\u77e5\u540d\u7684\u516c\u53f8\u5305\u62ec\u6280\u672f\u63d0\u4f9b\u5546\u70ed\u7535\u516c\u53f8(ThermoElectronCorporation)\u73b0\u4e3a\u8d5b\u9ed8\u98de\u4e16\u5c14\u79d1\u6280\u516c\u53f8(ThermoFis<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">herScientifi\u3001\u5b89\u6377\u4f26\u79d1\u6280\u6709\u9650\u516c\u53f8\u3001Illumina\u516c\u53f8\u7b49\uff1b\u7f8e\u56fd\u901a\u7528\u7535\u6c14\u516c\u53f8\uff08GE\uff09\u3001\u5fb7\u56fd\u897f\u95e8\u5b50\u80a1\u4efd\u516c\u53f8\u7b49\uff1b\u836f\u54c1\u751f\u4ea7\u5546\u7f57\u6c0f\u516c\u53f8\u3001\u62dc\u8033\u96c6\u56e2\u3001\u96c5\u57f9\u5236\u836f\u6709\u9650\u516c\u53f8\u7b49\uff0c\u5e02\u573a\u96c6\u4e2d\u5ea6\u5747\u8f83\u9ad8\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;color: rgb(255, 169, 0);font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\uff08\u4e8c\uff09\u4e2d\u56fd\u5206\u5b50\u8bca\u65ad\u53d1\u5c55\u901f\u5ea6\u9ad8\u4e8e\u5168\u7403\uff0c\u5e02\u573a\u524d\u666f\u5e7f\u9614<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u4e2d\u56fd\u7684\u5206\u5b50\u8bca\u65ad\u6280\u672f\u572820\u4e16\u7eaa\u516d\u4e03\u5341\u5e74\u4ee3\u5f00\u59cb\u840c\u82bd\uff0c80\u5e74\u4ee3\u51fa\u73b0\u57fa\u4e8e\u6838\u9178\u63a2\u9488\u7684\u653e\u5c04\u6027\u6838\u7d20\u6807\u8bb0\u3001\u70b9\u6742\u4ea4\u3001Southern\u5370\u8ff9\u6742\u4ea4\u548c\u9650\u5236\u6027\u7247\u6bb5\u957f\u5ea6\u591a\u6001\u6027\u8fde\u9501\u5206\u6790\u7b49\u5404\u79cd\u6280\u672f\uff0c90\u5e74\u4ee3\u968f\u7740PCR\u6280\u672f\u7684\u63a8\u5e7f\u548c\u5e94\u7528\uff0c\u5206\u5b50\u8bca\u65ad\u6280\u672f\u5728\u4e2d\u56fd\u5f97\u5230\u4e86\u8f83\u5feb\u53d1\u5c55\uff0c\u5f00\u59cb\u8fdb\u5165\u4e34\u5e8a\u5b9e\u9a8c\u5ba4\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">2013\u5e74\uff0c\u5168\u7403\u7684\u4f53\u5916\u8bca\u65ad\u5e02\u573a\u89c4\u6a21\u8fbe533\u4ebf\u7f8e\u5143\uff0c\u800c\u4e2d\u56fd\u7684\u4f53\u5916\u8bca\u65ad\u5e02\u573a\u89c4\u6a21\u5927\u7ea6\u4e3a200\u4ebf\u5143\u4eba\u6c11\u5e01[4]\u3002\u5728\u4f53\u5916\u8bca\u65ad\u884c\u4e1a\u7684\u5404\u4e2a\u5b50\u884c\u4e1a\u4e2d\uff0c\u751f\u5316\u8bca\u65ad\u6280\u672f\u5df2\u7ecf\u6210\u719f\uff0c\u56fd\u4ea7\u66ff\u4ee3\u57fa\u672c\u5b8c\u6210\uff0c\u5e74\u589e\u957f\u7387\u572810%\u4ee5\u4e0b\uff1b\u6700\u5148\u8fdb\u7684\u514d\u75ab\u8bca\u65ad\u6280\u672f\u5728\u5316\u5b66\u53d1\u5149\u7b49\u9886\u57df\uff0c\u884c\u4e1a\u589e\u957f\u7387\u572815%~20%\uff1b\u5373\u65f6\u68c0\u9a8c\uff08POCT\uff09\u56e0\u5176\u65b9\u4fbf\u3001\u5feb\u6377\u3001\u7ecf\u6d4e\u7684\u4f18\u70b9\uff0c\u589e\u957f\u7387\u8fbe\u523024%\u5de6\u53f3\uff1b\u800c\u5206\u5b50\u8bca\u65ad\u76ee\u524d\u5904\u4e8e\u6210\u957f\u671f\uff0c\u6210\u4e3aIVD\u884c\u4e1a\u589e\u957f\u6700\u5feb\u7684\u7ec6\u5206\u5b50\u884c\u4e1a\uff0c\u5e74\u589e\u957f\u7387\u9ad8\u8fbe\u7ea630%\uff0c\u5982\u56fe1\u6240\u793a[5],\u662f\u5168\u7403\u5e74\u5747\u589e\u957f\u901f\u5ea6\u76842\u500d\u3002\u5c3d\u7ba1\u6211\u56fd\u7684\u5206\u5b50\u8bca\u65ad\u5e02\u573a\u89c4\u6a21\u8f83\u5c0f\uff0c\u4f46\u589e\u957f\u8fc5\u901f\uff0c\u5e02\u573a\u89c4\u6a21\u4ece2010\u5e74\u768416.5\u4ebf\u5143\u589e\u957f\u81f32014\u5e74\u7684\u7ea645.9\u4ebf\u5143\uff0c\u590d\u5408\u589e\u957f\u7387\u8fbe29.1%\uff0c\u5360IVD\u5e02\u573a\u7684\u6bd4\u4f8b\u4ece2010\u5e74\u768411%\u589e\u957f\u81f32014\u5e74\u768415%\uff0c\u5206\u5b50\u8bca\u65ad\u5e02\u573a\u589e\u957f\u7387\u9ad8\u4e8eIVD\u6574\u4f53\u589e\u957f\u7387[5]\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u7ecf\u8fc720\u591a\u5e74\u7684\u53d1\u5c55\uff0c\u6211\u56fd\u7684\u5206\u5b50\u8bca\u65ad\u884c\u4e1a\u73b0\u5df2\u5177\u5907\u4ea7\u4e1a\u89c4\u6a21\u53d1\u5c55\u6761\u4ef6\u3002\u76ee\u524d\uff0c\u4ece\u4e8b\u5206\u5b50\u8bca\u65ad\u76f8\u5173\u4ea7\u54c1\u7814\u7a76\u548c\u5f00\u53d1\u7684\u4f01\u4e1a\u548c\u673a\u6784\u6709150\u591a\u5bb6\uff0c\u5176\u4e2d\u6bd4\u8f83\u6709\u7279\u8272\u7684\u5206\u5b50\u8bca\u65ad\u4f01\u4e1a\u5305\u62ec\u535a\u5965\u751f\u7269\u96c6\u56e2\u6709\u9650\u516c\u53f8\u3001\u8fbe\u5b89\u57fa\u56e0\u80a1\u4efd\u6709\u9650\u516c\u53f8\u3001\u8054\u5408\u57fa\u56e0\u79d1\u6280\u96c6\u56e2\u3001\u534e\u5927\u57fa\u56e0\u7b49\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u4e0e\u5168\u7403\u5206\u5b50\u8bca\u65ad\u5e02\u573a\u7c7b\u4f3c\uff0cPCR\u4e5f\u662f\u56fd\u5185\u5206\u5b50\u8bca\u65ad\u6700\u6210\u719f\u7684\u6280\u672f\uff0c\u5360\u636e\u6700\u5927\u7684\u5e02\u573a\u4efd\u989d\uff08\u7ea640%\uff09[5]\u3002\u57fa\u56e0\u82af\u7247\u6280\u672f\u548c\u5fae\u6d41\u63a7\u82af\u7247\u4e8c\u4ee3\u6d4b\u5e8f\u6280\u672f\u9010\u6e10\u8d70\u4e0a\u821e\u53f0\uff0c\u5177\u6709\u5f88\u597d\u7684\u53d1\u5c55\u524d\u666f\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;color: rgb(255, 169, 0);font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\uff08\u4e09\uff09\u4f53\u5916\u8bca\u65ad\u65b0\u6280\u672f\u5c42\u51fa\u4e0d\u7a77\u3001\u53d1\u5c55\u8fc5\u731b<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u5c3d\u7ba1\u88ab\u5e02\u573a\u8ba4\u53ef\u4e14\u76f8\u5bf9\u6210\u719f\u7684\u6280\u672f\u4ecd\u4ee5PCR\u4e3a\u4e3b\uff0c\u4f53\u5916\u8bca\u65ad\u9886\u57df\u5df2\u4ea7\u751f\u4e86\u4e00\u7cfb\u5217\u65b0\u7684\u6280\u672f\uff0c\u4f5c\u4e3a\u672a\u6765\u8fdb\u5165\u4e34\u5e8a\u8f6c\u5316\u7684\u50a8\u5907\u529b\u91cf\uff0c\u6f5c\u529b\u5341\u8db3\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">1.PCR\u6280\u672f\u4e0d\u65ad\u521b\u65b0\u7ecf\u8fc7\u6570\u5e74\u7684\u53d1\u5c55\uff0c\u4eba\u4eec\u5df2\u5728\u5e38\u89c4PCR\u7684\u57fa\u7840\u4e0a\u884d\u751f\u51fa\u4e86\u591a\u91cdPCR(multiplexPCR)\u3001\u5de2\u5f0fPCR\uff08nestedPCR\uff09\u3001\u53cd\u8f6c\u5f55PCR\uff08reversetranscriptionPCR\uff09\u548c\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u548c\u6570\u5b57PCR\uff08digitalPCR\uff09\u7b49\u591a\u79cd\u6280\u672f\u3002\u53cd\u8f6c\u5f55PCR\u4f7f\u5206\u5b50\u8bca\u65ad\u4e0d\u518d\u5c40\u9650\u4e8eDNA\u6c34\u5e73\uff0c\u800c\u53ef\u4ee5\u5728\u6838\u7cd6\u6838\u9178\uff08RNA\uff09\u6c34\u5e73\u68c0\u6d4b\u7f3a\u9677\u57fa\u56e0\uff0c\u5bf9\u4e8e\u4e00\u4e9b\u4ee5RNA\u4f5c\u4e3a\u9057\u4f20\u7269\u8d28\u7684\u75c5\u6bd2\u7b49\u75c5\u539f\u4f53\u7684\u68c0\u6d4b\u66f4\u52a0\u65b9\u4fbf\u3002\u76ee\u524d\u5e94\u7528\u6700\u5e7f\u6cdb\u7684\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u6280\u672f\u5728\u6838\u9178\u6269\u589e\u7684\u540c\u65f6\u52a0\u5165\u4e86\u7279\u5f02\u6027\u8367\u5149\u63a2\u9488\uff0c\u53ef\u5b9e\u65f6\u76d1\u6d4b\u6269\u589e\u8fc7\u7a0b\uff0c\u5b9e\u73b0\u4e86\u5b9a\u91cf\u68c0\u6d4b\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u6570\u5b57PCR(digitalPCR)\u662f\u57fa\u4e8e\u5355\u5206\u5b50PCR\u65b9\u6cd5\u5bf9DNA\u8fdb\u884c\u7edd\u5bf9\u5b9a\u91cf\u7684\u5de5\u5177\uff0c\u501f\u52a9\u5fae\u6d41\u63a7\u6280\u672f\u5c06\u5927\u91cf\u7a00\u91ca\u540e\u7684DNA\u5206\u6563\u81f3\u5fae\u53cd\u5e94\u5668\u4e2d\uff0c\u6bcf\u4e2a\u53cd\u5e94\u5668\u7684DNA\u6a21\u677f\u6570\u91cf\u5c11\u4e8e\u7b49\u4e8e1\u4e2a[6]\u3002\u7ecf\u8fc7PCR\u540e\uff0c\u5bf9\u6bcf\u4e2a\u5fae\u53cd\u5e94\u5b54\u7684\u8367\u5149\u4fe1\u53f7\u8fdb\u884c\u68c0\u6d4b\uff0c\u6709\u9776\u5e8f\u5217\u6a21\u677f\u7684\u5b54\u4f1a\u53d1\u51fa\u8367\u5149\uff0c\u6ca1\u6709\u9776\u5e8f\u5217\u6a21\u677f\u7684\u5b54\u6ca1\u6709\u8367\u5149\uff0c\u4ee5\u6b64\u63a8\u7b97\u539f\u59cb\u6eb6\u6db2\u4e2d\u5f85\u6d4b\u6838\u9178\u7684\u6d53\u5ea6\u3002Quantalife\u516c\u53f8\uff082011\u5e74\u88abBio-rad\u6536\u8d2d\uff09\u7814\u53d1\u7684\u5fae\u6ef4\u5f0f\u6570\u5b57PCR\u4eea\u5668\uff0c\u76ee\u524d\u5df2\u88ab\u7528\u4e8e\u5fae\u91cf\u75c5\u539f\u5fae\u751f\u7269\u68c0\u6d4b\u3001\u5355\u7ec6\u80de\u57fa\u56e0\u8868\u8fbe\u68c0\u6d4b\u7b49\u591a\u4e2a\u9886\u57df\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">2.\u975e\u6838\u9178\u7c7b\u68c0\u6d4b\u6280\u672f\u53d6\u5f97\u8fdb\u5c55\u9664\u4e86\u5bf9\u6838\u9178\u7684\u76f4\u63a5\u68c0\u6d4b\uff0c\u5bf9\u86cb\u767d\u8d28\u7b49\u975e\u6838\u9178\u7269\u8d28\u7684\u68c0\u6d4b\u4e5f\u662f\u5206\u5b50\u8bca\u65ad\u7684\u91cd\u8981\u5185\u5bb9\u4e4b\u4e00\u3002\u86cb\u767d\u82af\u7247\u53ef\u4ee5\u6781\u5927\u5730\u63d0\u9ad8\u86cb\u767d\u68c0\u6d4b\u7684\u901a\u91cf\uff0c\u5176\u57fa\u672c\u539f\u7406\u662f\u5c06\u86cb\u767d\u8d28\u6709\u5e8f\u5730\u56fa\u5b9a\u4e8e\u8f7d\u73bb\u7247\u7b49\u56fa\u76f8\u4ecb\u8d28\u4e0a\uff0c\u7136\u540e\u7528\u8367\u5149\u6807\u8bb0\u7684\u6297\u4f53\u4e0e\u82af\u7247\u4f5c\u7528\uff0c\u6297\u4f53\u4e0a\u7684\u8367\u5149\u6307\u793a\u5bf9\u5e94\u7684\u86cb\u767d\u8d28\u53ca\u5176\u8868\u8fbe\u91cf\uff0c\u53ef\u4ee5\u5b9e\u73b0\u9ad8\u901a\u91cf\u7684\u86cb\u767d\u68c0\u6d4b\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">3.\u6d4b\u5e8f\u6280\u672f\u65b9\u5174\u672a\u827e<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u6d4b\u5e8f\u53cd\u5e94\u662f\u53ef\u4ee5\u76f4\u63a5\u83b7\u5f97\u6838\u9178\u5e8f\u5217\u7684\u552f\u4e00\u624b\u6bb5\uff0c\u662f\u5206\u5b50\u8bca\u65ad\u6280\u672f\u7684\u4e00\u9879\u91cd\u8981\u5206\u652f\u30021977\u5e74Sanger\u53d1\u660e\u7684\u672b\u7aef\u7ec8\u6b62\u6cd5\u662f\u6700\u4e3a\u7ecf\u5178\u7684\u7b2c\u4e00\u4ee3\u6d4b\u5e8f\u6280\u672f\uff0c\u76ee\u524d\u4ecd\u662f\u83b7\u5f97\u6838\u9178\u5e8f\u5217\u4fe1\u606f\u7684\u6700\u5e38\u7528\u7684\u65b9\u6cd5\u3002200\u5e74454LifeSciences\u516c\u53f8\uff08\u5df2\u88abRoche\u6536\u8d2d\uff09\u7387\u5148\u63a8\u51fa\u4e86\u57fa\u4e8e\u7126\u78f7\u9178\u6d4b\u5e8f\u7684\u9ad8\u901a\u91cf\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u7cfb\u7edf\uff0c\u5f00\u521b\u4e86\u7b2c\u4e8c\u4ee3\u6d4b\u5e8f\u7684\u5148\u6cb3\u3002\u4e0e\u7b2c\u4e00\u4ee3\u6d4b\u5e8f\u6280\u672f\u4e0d\u540c\uff0c\u7b2c\u4e8c\u4ee3\u6d4b\u5e8f\u6280\u672f\u662f\u8fb9\u5408\u6210\u8fb9\u6d4b\u5e8f\uff1a\u901a\u8fc7DNA\u7247\u6bb5\u5316\u540e\u6784\u6210DNA\u6587\u5e93,\u6587\u5e93\u4e0e\u8f7d\u4f53\u4ea4\u8054\u540e\u6269\u589e,\u5728\u8f7d\u4f53\u4e0a\u8fb9\u5408\u6210\u8fb9\u6d4b\u5e8f\u3002\u4e8c\u4ee3\u6d4b\u5e8f\u4f7f\u57fa\u56e0\u6d4b\u5e8f\u7684\u901a\u91cf\u8fc5\u901f\u6269\u5927\uff0c\u53ef\u4e00\u6b21\u5b8c\u6210\u5bf9\u6d77\u91cf\u6570\u636e\u7684\u68c0\u6d4b\uff0c\u4e5f\u79f0\u4e3a\u6df1\u5ea6\u6d4b\u5e8f\uff08deepsequencing\uff09\u6216\u4e0b\u4e00\u4ee3\u6d4b\u5e8f\uff08nextgenerationsequencing\uff09\u3002\u7ecf\u8fc710\u591a\u5e74\u7684\u53d1\u5c55\uff0c\u4e8c\u4ee3\u6d4b\u5e8f\u65e5\u8d8b\u6210\u719f\u5e76\u5f00\u59cb\u5546\u4e1a\u5316\uff0c\u5e38\u89c1\u7684\u5e73\u53f0\u5305\u62ecRoche454\u3001IlluminaSolexa\u3001ABISOLID\u548cLifeIonTorrent\u7b49\uff0c\u76ee\u524dRoche454\u548cABISOLID\u5df2\u7ecf\u9010\u6e10\u88ab\u6dd8\u6c70\u3002\u5728\u5bf9\u75c5\u539f\u5fae\u751f\u7269\u8fdb\u884c\u5b8f\u57fa\u56e0\u7ec4\u9274\u5b9a\u3001\u5206\u6790\u6bcd\u4eb2\u8840\u6db2\u4e2d\u80ce\u513f\u7684DNA\u4fe1\u606f\u8fdb\u884c\u4ea7\u524d\u8bca\u65ad\u3001\u7ed8\u5236\u836f\u7269\u57fa\u56e0\u7ec4\u56fe\u8c31\u6307\u5bfc\u4e2a\u4f53\u5316\u7528\u836f\u7b49\u65b9\u9762\u5df2\u7ecf\u53d6\u5f97\u4e86\u7a81\u7834\u3002\u968f\u7740\u4e8c\u4ee3\u6d4b\u5e8f\u9010\u6e10\u666e\u53ca\u800c\u4ea7\u751f\u7684\u6d77\u91cf\u6570\u636e\uff0c\u4e5f\u5bf9\u540e\u7eed\u7684\u751f\u7269\u4fe1\u606f\u5b66\u53d1\u5c55\u63d0\u51fa\u4e86\u65b0\u7684\u8981\u6c42\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u5728\u7b2c\u4e8c\u4ee3\u6d4b\u5e8f\u4e0d\u65ad\u5b8c\u5584\u548c\u5e7f\u6cdb\u5e94\u7528\u7684\u540c\u65f6\uff0c\u4ee5\u5355\u5206\u5b50DNA\u6d4b\u5e8f\u4e3a\u7279\u5f81\u7684\u7b2c\u4e09\u4ee3\u6d4b\u5e8f\u6280\u672f\u5e94\u8fd0\u800c\u751f\u30022008\u5e74\uff0cHelicoBioScience\u516c\u53f8\u9996\u5148\u62a5\u9053\u4e86\u57fa\u4e8e\u5168\u5185\u53cd\u5c04\u663e\u5fae\u955c\uff08TIRM)\u7684\u5355\u5206\u5b50\u6d4b\u5e8f\u6280\u672f[7]\uff0c\u73b0\u5728\u5df2\u7ecf\u6210\u529f\u63a8\u51fa\u7b2c\u4e09\u4ee3\u6d4b\u5e8f\u4eeaPacbioRS\u2161\u548cPacbioSequel\u3002\u4e4b\u540e\u53c8\u51fa\u73b0\u4e86PacificBioSciences\u516c\u53f8\u5f00\u53d1\u7684\u5355\u5206\u5b50\u5b9e\u65f6\u6280\u672f\uff08SMRT\uff09\u4ee5\u53caOxordNanoporeTechnologies\u516c\u53f8\u7684\u7eb3\u7c73\u5b54\u6280\u672f\u3002\u8fd9\u4e9b\u6280\u672f\u672a\u6765\u4e5f\u53ef\u80fd\u6210\u4e3a\u5206\u5b50\u8bca\u65ad\u7684\u751f\u529b\u519b\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">4.POCT\u7ee7\u7eed\u53d1\u5c55<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">POCT\u548c\u5206\u5b50\u8bca\u65ad\u662f\u4f53\u5916\u8bca\u65ad\u884c\u4e1a\u589e\u957f\u6700\u5feb\u7684\u4e24\u4e2a\u7ec6\u5206\u9886\u57df\u3002POCT\u4ea7\u54c1\u6709\u4e09\u4e2a\u7279\u70b9\uff1a\u68c0\u6d4b\u65f6\u95f4\u77ed\uff0c\u68c0\u6d4b\u7a7a\u95f4\u7075\u6d3b\uff0c\u68c0\u6d4b\u64cd\u4f5c\u8005\u53ef\u4ee5\u662f\u975e\u4e13\u4e1a\u4eba\u5458\uff0c\u751a\u81f3\u662f\u88ab\u68c0\u5bf9\u8c61\u672c\u4eba\u3002POCT\u5e73\u53f0\u7684\u53d1\u5c55\u76ee\u6807\u662f\u5c06\u591a\u6b65\u9aa4\u7684\u68c0\u6d4b\u5b8c\u5168\u6574\u5408\u5230\u4e00\u4e2a\u7cfb\u7edf\uff0c\u7528\u6237\u53ea\u9700\u8981\u52a0\u5165\u6837\u54c1\uff0c\u7cfb\u7edf\u5c31\u53ef\u4ee5\u5168\u81ea\u52a8\u5316\u5730\u5b8c\u6210\u5269\u4e0b\u6240\u6709\u6b65\u9aa4\uff0c\u5b9e\u73b0\u201c\u6837\u54c1\u8fdb\uff0c\u7ed3\u679c\u51fa\u201d\uff0c\u800cPOCT\u4ea7\u751f\u7684\u7ec8\u7aef\u6570\u636e\u901a\u8fc7\u4f20\u8f93\u5230\u8fbe\u533b\u7597\u673a\u6784\uff0c\u53ef\u4ee5\u5bf9\u88ab\u68c0\u4eba\u5458\u63d0\u4f9b\u8fdc\u7a0b\u533b\u7597\u670d\u52a1\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">5.\u5206\u5b50\u6210\u50cf\u6280\u672f\u9010\u6e10\u6210\u719f<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word 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style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;color: rgb(255, 169, 0);font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\uff08\u56db\uff09\u56fd\u5bb6\u653f\u7b56\u652f\u6301\u53ca\u5e02\u573a\u73af\u5883<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u8fd1\u4e9b\u5e74\u6765\uff0c\u6211\u56fd\u7684\u57fa\u56e0\u7ec4\u5b66\u7684\u76f8\u5173\u4ea7\u4e1a\u76d1\u7ba1\u73af\u5883\u7ecf\u5386\u4e86\u53d8\u5316\u5bbd\u677e\u653e\u4efb\u671f\uff082014\u5e74\u524d\uff09\u2014\u505c\u6b62\u671f\uff082014\u5e742\u6708\uff09\u2014\u90e8\u5206\u653e\u884c\u671f\uff082014\u5e746\u6708\u81f3\u5e74\u5e95\uff09\u2014\u9f13\u52b1\u53d1\u5c55\u671f\uff082015\u5e74\u81f3\u4eca\uff09\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u4f53\u5916\u8bca\u65ad\u662f\u201c\u5341\u4e09\u4e94\u89c4\u5212\u201d\u7684\u91cd\u8981\u53d1\u5c55\u9886\u57df\uff0c\u76d1\u7ba1\u90e8\u95e8\u5bf9\u884c\u4e1a\u6574\u4f53\u5448\u73b0\u9010\u6b65\u653e\u5f00\u548c\u652f\u6301\u7684\u6001\u5ea6\uff0c\u8fd9\u7ed9\u4f53\u5916\u8bca\u65ad\u884c\u4e1a\u5e26\u6765\u4e86\u96be\u5f97\u7684\u53d1\u5c55\u673a\u9047\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u968f\u7740\u533b\u4fdd\u63a7\u8d39\u7684\u538b\u529b\u589e\u5927\uff0c\u533b\u9662\u6536\u5165\u6765\u6e90\u5c06\u4ece\u836f\u54c1\u9010\u6e10\u5411\u533b\u7597\u670d\u52a1\u8f6c\u79fb\uff0c\u4f53\u5916\u8bca\u65ad\u5305\u62ec\u5206\u5b50\u8bca\u65ad\u7684\u884c\u4e1a\u5730\u4f4d\u5c06\u9010\u6b65\u63d0\u5347[5]\u3002\u540c\u65f6\uff0c2016\u5e741\u67085\u65e5\u56fd\u52a1\u9662\u5370\u53d1\u300a\u5173\u4e8e\u5b9e\u65bd\u5168\u9762\u4e24\u5b69\u653f\u7b56\u6539\u9769\u5b8c\u5584\u8ba1\u5212\u751f\u80b2\u670d\u52a1\u7ba1\u7406\u7684\u51b3\u5b9a\u300b\uff0c\u4e8c\u80ce\u653f\u7b56\u843d\u5730\uff0c\u4ec5\u65e0\u521b\u4ea7\u524d\u7b5b\u67e5\uff08NIPT\uff09\u9886\u57df\uff0c\u6bcf\u5e74\u65b0\u589e\u7684\u7b5b\u67e5\u91cf\u9884\u8ba1\u5c31\u5c06\u5e26\u67657\u4ebf\u5143\u7684\u5e02\u573a\u6536\u5165[5]\u3002\u53e6\u5916\uff0c\u968f\u7740\u6211\u56fd\u533b\u7597\u6539\u9769\u7684\u6df1\u5165\uff0c\u5c45\u6c11\u5065\u5eb7\u610f\u8bc6\u65e5\u76ca\u589e\u5f3a\uff0c\u4e5f\u7ed9\u5206\u5b50\u8bca\u65ad\u5e02\u573a\u5e26\u6765\u673a\u9047\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;color: rgb(255, 169, 0);font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\uff08\u4e94\uff09\u5206\u5b50\u8bca\u65ad\u7ec6\u5206\u5e02\u573a\u5206\u6790<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u6839\u636e\u5176\u6210\u719f\u5ea6\u548c\u5e02\u573a\u63a5\u53d7\u5ea6\uff0c\u5206\u5b50\u8bca\u65ad\u6280\u672f\u4e3b\u8981\u5e94\u7528\u4e8e\u56db\u4e2a\u65b9\u9762\uff1a\u611f\u67d3\u6027\u75be\u75c5\u3001\u9057\u4f20\u75c5\u3001\u80bf\u7624\u548c\u836f\u7269\u57fa\u56e0\u7ec4\u5b66\u68c0\u6d4b[10]\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word 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style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word 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style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><img class=\"\"       style=\"-webkit-tap-highlight-color: rgba(0, 0, 0, 0);border-style: none;box-sizing: border-box !important;word-wrap: break-word !important;visibility: visible !important;width: auto !important;\" width=\"auto\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/07\/beepress7-1532684612.jpg\" alt=\"\u4e2d\u56fd\u5206\u5b50\u8bca\u65ad\u4ea7\u4e1a\u6218\u7565\u7814\u7a76\" title=\"\u4e2d\u56fd\u5206\u5b50\u8bca\u65ad\u4ea7\u4e1a\u6218\u7565\u7814\u7a76\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<section style=\"max-width: 100%;box-sizing: border-box;color: rgb(51, 51, 51);\">\n<section class=\"\"  style=\"max-width: 100%;box-sizing: border-box;text-align: center;word-wrap: break-word !important;\">\n<section class=\"\" style=\"max-width: 100%;box-sizing: border-box;display: inline-block;vertical-align: top;word-wrap: break-word !important;\">\n<section style=\"margin-right: -1px;margin-left: auto;max-width: 100%;box-sizing: border-box;width: 12px;height: 12px;border-top: 3px solid rgb(255, 202, 0);border-right: 3px solid rgb(255, 202, 0);transform: rotate(0deg);word-wrap: break-word !important;\"><\/section>\n<section style=\"margin-top: -11px;margin-bottom: -11px;padding: 4px;max-width: 100%;box-sizing: border-box;border-width: 1px;border-style: solid;border-color: rgb(223, 223, 223);word-wrap: break-word !important;\">\n<section class=\"\" style=\"padding-right: 6px;padding-left: 6px;max-width: 100%;box-sizing: border-box;border-width: 1px;border-style: solid;border-color: rgb(223, 223, 223);font-size: 14px;word-wrap: break-word !important;\">\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box;word-wrap: 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!important;\">\u5728\u6211\u56fd\u8fd9\u6837\u7684\u65b0\u5174\u5e02\u573a\u4e0a\uff0c\u5206\u5b50\u8bca\u65ad\u5e02\u573a\u7684\u5feb\u901f\u589e\u957f\u4ee4\u6295\u8d44\u8005\u8d8b\u4e4b\u82e5\u9e5c\uff0c\u5927\u6279\u5916\u8d44\u4f01\u4e1a\u7eb7\u7eb7\u8fdb\u5165\u30022012\u5e741\u670817\u65e5\uff0cLifeTechnologies\u516c\u53f8\u548c\u8fbe\u5b89\u57fa\u56e0\u80a1\u4efd\u6709\u9650\u516c\u53f8\u7b7e\u8ba2\u5408\u4f5c\u534f\u8bae\uff0c\u5728\u6211\u56fd\u6210\u7acb\u4f53\u5916\u8bca\u65ad\u6280\u672f\u5408\u8d44\u4f01\u4e1a\u2014\u2014Life\u2013\u8fbe\u5b89\u8bca\u65ad\uff0c\u81f4\u529b\u4e8e\u63a8\u52a8\u9057\u4f20\u6027\u75be\u75c5\u3001\u764c\u75c7\u548c\u4f20\u67d3\u6027\u75be\u75c5\u7684\u4f53\u5916\u8bca\u65ad\u5206\u6790[14]\u3002\u540c\u65f6\u7f57\u6c0f\u516c\u53f8\u3001\u7f8e\u56fd\u4f2f\u4e50\u516c\u53f8\u3001\u51ef\u6770\u751f\u7269\u6280\u672f\u6709\u9650\u516c\u53f8\u3001Illumina\u3001PacificBio\u7b49\u56fd\u5916\u4f01\u4e1a\u7eb7\u7eb7\u4ee5\u5404\u79cd\u65b9\u5f0f\u6e17\u5165\u548c\u53c2\u4e0e\u4e2d\u56fd\u5206\u5b50\u8bca\u65ad\u5e02\u573a\uff0c\u8d62\u53d6\u5148\u53d1\u4f18\u52bf\uff0c\u5bf9\u56fd\u5185\u73b0\u6709\u7684\u5206\u5b50\u8bca\u65ad\u4f01\u4e1a\u7684\u53d1\u5c55\u9020\u6210\u4e86\u4e25\u91cd\u51b2\u51fb\u3002\u9762\u5bf9\u5916\u8d44\u9891\u9891\u5e03\u5c40\u56fd\u5185\u5206\u5b50\u8bca\u65ad\u5e02\u573a\u7684\u7d27\u8feb\u5f62\u52bf\uff0c\u6211\u56fd\u5fc5\u987b\u5c3d\u5feb\u8fdb\u884c\u6218\u7565\u7814\u7a76\u548c\u5e03\u5c40\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;color: rgb(255, 169, 0);font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\uff08\u4e8c\uff09\u5206\u5b50\u8bca\u65ad\u7684\u53d1\u5c55\u6613\u53d7\u76d1\u7ba1\u5236\u5ea6\u7684\u5236\u7ea6\uff0c\u5728\u6478\u7d22\u4e2d\u9010\u6b65\u53d1\u5c55<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word 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style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;color: rgb(255, 169, 0);font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\uff08\u4e09\uff09\u9762\u5bf9\u4e34\u5e8a\u4e0a\u57fa\u56e0\u68c0\u6d4b\u6280\u672f\u8d8a\u6765\u8d8a\u591a\u7684\u5e94\u7528\uff0c\u5982\u4f55\u89e3\u8bfb\u7ed9\u51fa\u4e34\u5e8a\u6307\u5bfc\u610f\u89c1\u6210\u4e3a\u533b\u52a1\u5de5\u4f5c\u8005\u7684\u5de8\u5927\u6311\u6218<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box 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style=\"max-width: 100%;color: rgb(255, 169, 0);font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\uff08\u56db\uff09\u4ea7\u4e1a\u53d1\u5c55\u653f\u7b56\u73af\u5883\u9700\u8fdb\u4e00\u6b65\u52a0\u5f3a\u548c\u6539\u9769<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word 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style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;color: rgb(255, 169, 0);font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\uff08\u4e94\uff09\u4ea7\u4e1a\u53d1\u5c55\u81ea\u8eab\u9762\u4e34\u7684\u6311\u6218<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word 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style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u533b\u4fdd\u653f\u7b56\u7684\u5f71\u54cd\uff1a\u5206\u5b50\u8bca\u65ad\u5e02\u573a\u7684\u62d3\u5c55\u548c\u65b0\u4ea7\u54c1\u7684\u63a8\u51fa\u5f88\u5927\u7a0b\u5ea6\u4e0a\u53d7\u533b\u4fdd\u53ca\u5546\u4e1a\u533b\u7597\u4fdd\u9669\u652f\u6301\u7a0b\u5ea6\u7684\u5f71\u54cd\u3002\u76ee\u524d\u7684\u533b\u7597\u4fdd\u9669\u66f4\u591a\u5730\u662f\u652f\u6301\u5df2\u53d1\u751f\u75be\u75c5\u7684\u6cbb\u7597\uff0c\u800c\u5bf9\u75be\u75c5\u7684\u65e9\u671f\u8bca\u65ad\u548c\u9884\u6d4b\u7f3a\u4e4f\u652f\u6301\uff0c\u56e0\u800c\u9650\u5236\u4e86\u5206\u5b50\u8bca\u65ad\u4ea7\u54c1\u7684\u63a8\u5e7f[16]\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u4ea7\u54c1\u6216\u6280\u672f\u88ab\u6dd8\u6c70\u7684\u98ce\u9669\uff1a\u5f53\u524d\u57fa\u56e0\u82af\u7247\u3001\u5fae\u6d41\u63a7\u82af\u7247\u6d4b\u5e8f\u7b49\u6280\u672f\u662f\u5b9e\u65f6\u8367\u5149\u5b9a\u91cfPCR\u68c0\u6d4b\u6280\u672f\u7684\u6f5c\u5728\u7ade\u4e89\u6280\u672f\uff0c\u53ef\u80fd\u5728\u5c06\u6765\u53d6\u4ee3\u73b0\u6709\u6280\u672f[16]\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word 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!important;\">\u5206\u5b50\u8bca\u65ad\u884c\u4e1a\u7684\u95e8\u69db\u4e4b\u4e00\u662f\u7279\u5b9a\u77e5\u8bc6\u4ea7\u6743\u548c\u975e\u4e13\u5229\u6280\u672f\uff0c\u800c\u6211\u56fd\u5bf9\u77e5\u8bc6\u4ea7\u6743\u7684\u4fdd\u62a4\u529b\u5ea6\u4e0d\u591f\uff0c\u56e0\u6b64\uff0c\u4f01\u4e1a\u9700\u8981\u627f\u62c5\u638c\u63e1\u6838\u5fc3\u6280\u672f\u7684\u4eba\u624d\u6d41\u5931\u53ca\u6838\u5fc3\u6280\u672f\u6cc4\u5bc6\u7684\u98ce\u9669[16]\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;color: rgb(255, 169, 0);box-sizing: border-box !important;word-wrap: break-word !important;\">\uff08\u4e03\uff09\u56fd\u5bb6\u76d1\u7ba1\u95ee\u9898<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u56fd\u5bb6\u5bf9\u90e8\u5206\u4ea7\u54c1\uff0c\u5c24\u5176\u662f\u5bf9\u8bca\u65ad\u8bd5\u5242\u4ea7\u54c1\u548c\u7b2c\u4e09\u65b9\u670d\u52a1\u7684\u8fc7\u5ea6\u76d1\u7ba1\uff0c\u4f1a\u7ed9\u4f9b\u9700\u53cc\u65b9\u5e26\u6765\u5f88\u591a\u95ee\u9898\uff0c\u5f62\u6210\u74f6\u9888\u3002<\/span><\/p>\n<section style=\"max-width: 100%;color: rgb(51, 51, 51);\">\n<section class=\"\"  style=\"max-width: 100%;text-align: center;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<p style=\"max-width: 100%;min-height: 1em;box-sizing: border-box !important;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<section class=\"\" style=\"max-width: 100%;box-sizing: border-box;display: inline-block;vertical-align: top;word-wrap: break-word !important;\">\n<section style=\"margin-right: -1px;margin-left: auto;max-width: 100%;box-sizing: border-box;width: 12px;height: 12px;border-top: 3px solid rgb(255, 202, 0);border-right: 3px solid rgb(255, 202, 0);transform: rotate(0deg);word-wrap: break-word !important;\"><\/section>\n<section style=\"margin-top: -11px;margin-bottom: -11px;padding: 4px;max-width: 100%;box-sizing: border-box;border-width: 1px;border-style: solid;border-color: rgb(223, 223, 223);word-wrap: break-word !important;\">\n<section class=\"\" style=\"padding-right: 6px;padding-left: 6px;max-width: 100%;box-sizing: border-box;border-width: 1px;border-style: solid;border-color: rgb(223, 223, 223);font-size: 14px;word-wrap: break-word !important;\">\n<p style=\"max-width: 100%;box-sizing: border-box;min-height: 1em;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\u56db\u3001\u5bf9\u7b56\u5efa\u8bae<\/strong><\/p>\n<\/section>\n<\/section>\n<section style=\"margin-left: -1px;max-width: 100%;box-sizing: border-box;width: 12px;height: 12px;border-bottom: 3px solid rgb(255, 202, 0);border-left: 3px solid rgb(255, 202, 0);transform: rotate(0deg);word-wrap: break-word !important;\"><\/section>\n<\/section>\n<p style=\"max-width: 100%;min-height: 1em;box-sizing: border-box !important;word-wrap: break-word !important;\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<\/section>\n<\/section>\n<section class=\"\"  style=\"max-width: 100%;color: rgb(51, 51, 51);\">\n<p style=\"max-width: 100%;min-height: 1em;line-height: 1.75em;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;color: rgb(255, 169, 0);font-size: 14px;box-sizing: border-box !important;word-wrap: 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100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\uff082\uff09\u9488\u5bf9\u521b\u65b0\u578b\u5206\u5b50\u8bca\u65ad\u4ea7\u54c1\u548c\u670d\u52a1\uff0c\u5236\u5b9a\u65b0\u7684\u7269\u4ef7\u5ba1\u6279\u3001\u533b\u4fdd\u793e\u4fdd\u548c\u653f\u5e9c\u91c7\u8d2d\u653f\u7b56\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;color: rgb(255, 169, 0);font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\uff08\u4e8c\uff09\u9f13\u52b1\u5e76\u8d2d\u5408\u4f5c\u5f62\u6210\u5b8c\u5907\u7684\u4ea7\u4e1a\u94fe<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u4ece\u5206\u5b50\u8bca\u65ad\u4ea7\u4e1a\u94fe\u5206\u6790\uff0c\u76ee\u524d\u56fd\u5185\u63d0\u4f9b\u8bd5\u5242\u76d2\u548c\u4eea\u5668\u4ea7\u54c1\u4ee5\u53ca\u63d0\u4f9b\u5206\u5b50\u8bca\u65ad\u68c0\u6d4b\u670d\u52a1\u7684\u516c\u53f8\u5f88\u591a\uff0c\u800c\u539f\u6750\u6599\u548c\u9ad8\u7aef\u4eea\u5668\u7684\u7814\u53d1\u548c\u751f\u4ea7\u7684\u516c\u53f8\u8f83\u5c11\u3002\u4e0d\u540c\u4ea7\u4e1a\u94fe\u73af\u8282\u7684\u516c\u53f8\u53ef\u91c7\u53d6\u5dee\u5f02\u5316\u53d1\u5c55\u7b56\u7565[17]\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word 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style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">3.\u63d0\u4f9b\u91d1\u878d\u653f\u7b56\u652f\u6301\u5bf9\u5206\u5b50\u8bca\u65ad\u6280\u672f\u7684\u5f00\u53d1\u3001\u4ea7\u54c1\u7814\u5236\u548c\u5e94\u7528\u63a8\u5e7f\uff0c\u5efa\u7acb\u79ef\u6781\u548c\u826f\u6027\u7684\u524d\u3001\u4e2d\u3001\u540e\u671f\u6295\u8d44\u673a\u5236\uff0c\u6210\u7acb\u4ea7\u4e1a\u5f15\u5bfc\u57fa\u91d1\uff0c\u7ed9\u4e88\u76f8\u5173\u7814\u53d1\u548c\u751f\u4ea7\u4f01\u4e1a\u4e00\u5b9a\u7684\u8d37\u6b3e\u514d\u606f\u653f\u7b56\uff0c\u5927\u529b\u652f\u6301\u77e5\u8bc6\u4ea7\u6743\u62b5\u62bc\u3001\u5165\u80a1\u7b49\uff0c\u5145\u5206\u4f53\u73b0\u77e5\u8bc6\u4ea7\u6743\u7684\u4ef7\u503c\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  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style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">5.\u63d0\u5347\u76d1\u7ba1\u6c34\u5e73\u8fc5\u901f\u6269\u5927\u548c\u52a0\u5f3a\u4ea7\u54c1\u8bc4\u5ba1\u548c\u76d1\u7ba1\u961f\u4f0d\u5efa\u8bbe\uff0c\u63d0\u9ad8\u8bc4\u5ba1\u548c\u76d1\u7ba1\u6c34\u5e73\uff0c\u52a0\u5feb\u521b\u65b0\u4ea7\u54c1\u7684\u5ba1\u6279\u7a0b\u5e8f\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;color: rgb(255, 169, 0);font-size: 14px;box-sizing: border-box 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style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\uff081\uff09\u5efa\u8bae\u5728\u56fd\u5bb6\u5c42\u9762\u521b\u5efa\u4e00\u6279\u56fd\u9645\u8ba4\u53ef\u7684\u5177\u5907\u53ef\u4ee5\u5bf9\u5206\u5b50\u8bca\u65ad\u4ea7\u54c1\u8fdb\u884c\u751f\u7269\u5b66\u8bc4\u4ef7\u3001\u901a\u7528\u5b89\u5168\u8981\u6c42\u6d4b\u8bc4\u548c\u8ba4\u8bc1\u8d44\u8d28\u7684\u5b9e\u9a8c\u5ba4\u6216\u673a\u6784\uff0c\u5efa\u7acb\u4e25\u683c\u7684\u5ba1\u67e5\u7ba1\u7406\u673a\u5236\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\uff082\uff09\u52a0\u5f3a\u5bf9\u5ba1\u67e5\u4eba\u5458\u548c\u5ba1\u67e5\u4e13\u5bb6\u7684\u57f9\u8bad\uff0c\u8fc5\u901f\u63d0\u9ad8\u5ba1\u67e5\u4eba\u5458\u548c\u5ba1\u67e5\u4e13\u5bb6\u7684\u7d20\u8d28\u548c\u4e1a\u52a1\u6c34\u5e73\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 14px;box-sizing: border-box !important;word-wrap: break-word !important;\">\uff083\uff09\u5efa\u8bae\u4ece\u56fd\u5bb6\u5c42\u9762\u9f13\u52b1\u7b2c\u4e09\u65b9\u5206\u5b50\u8bca\u65ad\u4e34\u5e8a\u54a8\u8be2\u673a\u6784\u7684\u5efa\u7acb\u3002<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 12px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u3010\u4f5c\u8005\u3011\u6e05\u534e\u5927\u5b66\u533b\u5b66\u9662\u533b\u5b66\u7cfb\u7edf\u751f\u7269\u5b66\u7814\u7a76\u4e2d\u5fc3\u8c22\u5170\uff0c\u5218\u5189\uff0c\u51af\u5a1f\uff0c\u90a2\u5a49\u4e3d\uff0c\u7a0b\u4eac<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><span style=\"max-width: 100%;font-size: 12px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 12px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u3010\u6765\u6e90\u3011<\/span><span style=\"max-width: 100%;font-size: 12px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u4e2d\u56fd\u5de5\u7a0b\u79d1\u5b66\uff082017\u5e74\u7b2c19\u5377\u7b2c2\u671f\uff09<\/span><\/span><\/p>\n<section class=\"\"   style=\"max-width: 100%;box-sizing: border-box;\">\n<p style=\"max-width: 100%;min-height: 1em;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 12px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u3010\u5168\u6587\u6574\u7406\u3011\u5965\u54a8\u8fbe<\/span><\/p>\n<p style=\"max-width: 100%;min-height: 1em;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;font-size: 12px;box-sizing: border-box !important;word-wrap: break-word !important;\">\u3010\u58f0\u660e\u3011\u90e8\u5206\u6587\u7ae0\u548c\u4fe1\u606f\u6765\u6e90\u4e8e\u4e92\u8054\u7f51\uff0c\u4e0d\u4ee3\u8868\u672c\u8ba2\u9605\u53f7\u8d5e\u540c\u5176\u89c2\u70b9\u548c\u5bf9\u5176\u771f\u5b9e\u6027\u8d1f\u8d23\u3002\u5982\u8f6c\u8f7d\u5185\u5bb9\u6d89\u53ca\u7248\u6743\u7b49\u95ee\u9898\uff0c\u8bf7\u7acb\u5373\u4e0e\u6211\u4eec\u8054\u7cfb\uff0c\u6211\u4eec\u5c06\u8fc5\u901f\u91c7\u53d6\u9002\u5f53\u63aa\u65bd\u3002<\/span><\/p>\n<p><span style=\"max-width: 100%;font-size: 12px;box-sizing: border-box !important;word-wrap: break-word !important;\"><br  \/><\/span><\/p>\n<\/section>\n<section class=\"\"   style=\"max-width: 100%;box-sizing: border-box;color: rgb(51, 51, 51);\">\n<section  class=\"\" style=\"max-width: 100%;box-sizing: border-box;border-width: 0px;border-style: none;border-color: initial;word-wrap: break-word !important;\">\n<section style=\"padding: 10px;max-width: 100%;box-sizing: border-box;word-wrap: break-word !important;\">\n<section  style=\"margin: 20px auto;max-width: 100%;width: 650px;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section  style=\"margin: -20px auto;max-width: 100%;width: 617.5px;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<p style=\"max-width: 100%;min-height: 1em;text-align: center;box-sizing: border-box !important;word-wrap: break-word !important;\"><img class=\"__bg_gif\"     style=\"display: inline;box-sizing: border-box !important;word-wrap: break-word !important;visibility: visible !important;width: auto !important;\" title=\"\u4e2d\u56fd\u5206\u5b50\u8bca\u65ad\u4ea7\u4e1a\u6218\u7565\u7814\u7a76\" width=\"auto\" src=\"http:\/\/www.05921.net\/wp-content\/uploads\/2018\/07\/beepress10-1532684612.gif\" alt=\"\u4e2d\u56fd\u5206\u5b50\u8bca\u65ad\u4ea7\u4e1a\u6218\u7565\u7814\u7a76\"  \/><\/p>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\"><br style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"  \/><\/p>\n<section class=\"\"   style=\"max-width: 100%;box-sizing: border-box;color: rgb(51, 51, 51);\">\n<section class=\"\" style=\"max-width: 100%;box-sizing: border-box;border-width: 0px;border-style: none;border-color: initial;word-wrap: break-word !important;\">\n<section  style=\"max-width: 100%;width: 670px;box-sizing: border-box !important;word-wrap: break-word !important;\">\n<section class=\"\" style=\"margin-top: -25px;margin-right: auto;margin-left: auto;padding: 15px;max-width: 100%;box-sizing: border-box;line-height: 28px;background-color: rgb(223, 253, 254);word-wrap: break-word !important;\">\n<p style=\"max-width: 100%;min-height: 1em;box-sizing: border-box !important;word-wrap: break-word !important;\"><a href=\"https:\/\/mp.weixin.qq.com\/s?__biz=MzIwMTQ3MDc4Mw==&amp;mid=2247492103&amp;idx=2&amp;sn=f7f02650c656312b1da81c9c36fb6fec&amp;chksm=96efc457a1984d41ed5da1f82e24e9892be681d36ef2c68514794f9b95f69001fcaee5e5b66a&amp;scene=21#wechat_redirect\" target=\"_blank\" style=\"color: rgb(255, 41, 65);text-decoration: underline;-webkit-tap-highlight-color: rgba(0, 0, 0, 0);max-width: 100%;font-size: 15px;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\">\u57fa\u56e0\u4ff1\u4e50\u90e8\u2014\u57fa\u56e0\u79d1\u6280\u884c\u4e1a\u3001\u4e13\u4e1a\u53ca\u6295\u8d44\u4eba\u58eb\u7684\u79c1\u5bc6\u4e16\u754c\uff0c\u4e00\u4e2a\u6709\u4ef7\u503c\u7684\u57fa\u56e0\u79d1\u6280\u8d44\u8baf\u3001\u4ea4\u6d41\u4e0e\u4e92\u52a8\u5e73\u53f0\u3002<\/strong><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><\/strong><\/span><\/a><\/p>\n<p><span style=\"max-width: 100%;color: rgb(0, 82, 255);font-size: 15px;box-sizing: border-box !important;word-wrap: break-word !important;\"><strong style=\"max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><a href=\"https:\/\/mp.weixin.qq.com\/s?__biz=MzIwMTQ3MDc4Mw==&amp;mid=2247492103&amp;idx=2&amp;sn=f7f02650c656312b1da81c9c36fb6fec&amp;chksm=96efc457a1984d41ed5da1f82e24e9892be681d36ef2c68514794f9b95f69001fcaee5e5b66a&amp;scene=21#wechat_redirect\" target=\"_blank\" style=\"color: rgb(87, 107, 149);-webkit-tap-highlight-color: rgba(0, 0, 0, 0);max-width: 100%;box-sizing: border-box !important;word-wrap: break-word !important;\"><\/a><a href=\"http:\/\/mp.weixin.qq.com\/s?__biz=MzIwMTQ3MDc4Mw==&amp;mid=2247492103&amp;idx=2&amp;sn=f7f02650c656312b1da81c9c36fb6fec&amp;chksm=96efc457a1984d41ed5da1f82e24e9892be681d36ef2c68514794f9b95f69001fcaee5e5b66a&amp;scene=21#wechat_redirect\" target=\"_blank\" style=\"color: rgb(0, 82, 255);text-decoration: underline;-webkit-tap-highlight-color: rgba(0, 0, 0, 0);max-width: 100%;font-family: inherit;font-style: inherit;font-variant-caps: inherit;border-width: 0px;border-style: initial;border-color: initial;font-stretch: inherit;line-height: inherit;vertical-align: baseline;word-break: break-word;box-sizing: border-box !important;word-wrap: break-word !important;\"><span style=\"max-width: 100%;border-width: 0px;border-style: initial;border-color: initial;font-family: inherit;font-style: inherit;font-variant-caps: inherit;font-stretch: inherit;line-height: inherit;vertical-align: baseline;word-break: break-word;box-sizing: border-box !important;word-wrap: break-word !important;\">\u70b9\u51fb\u52a0\u5165\u57fa\u56e0\u4ff1\u4e50\u90e8\uff0c\u6210\u5c31\u884c\u4e1a\u7cbe\u82f1\uff0c\u5c0a\u4eab\u57fa\u56e0\u76db\u5bb4\uff01<\/span><\/a><\/strong><\/span><\/section>\n<\/section>\n<\/section>\n<\/section>\n<p style=\"max-width: 100%;min-height: 1em;color: rgb(51, 51, 51);\">\n<blockquote class='keep-source'>\n<p>\u59cb\u53d1\u4e8e\u5fae\u4fe1\u516c\u4f17\u53f7\uff1a                         \u57fa\u56e0\u8c37                      <\/p>\n<\/blockquote>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u6458\u8981\uff1a\u5206\u5b50\u8bca\u65ad\u662f\u4e2a\u4f53\u5316\u533b\u5b66\u7684\u91cd\u8981\u7ec4\u6210\u90e8\u5206\uff0c\u5df2\u6210\u4e3a\u4f53\u5916\u8bca\u65ad\u884c\u4e1a\u7684\u4e3b\u8981\u589e\u957f\u52a8\u529b\u3002\u8fd1\u5e74\u6765\uff0c\u5c3d\u7ba1\u6211\u56fd\u7684\u5206\u5b50\u8bca\u65ad\u5e02\u573a\u89c4\u6a21 [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":9999,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[2],"tags":[],"_links":{"self":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/9998"}],"collection":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/comments?post=9998"}],"version-history":[{"count":1,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/9998\/revisions"}],"predecessor-version":[{"id":10003,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/posts\/9998\/revisions\/10003"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media\/9999"}],"wp:attachment":[{"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/media?parent=9998"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/categories?post=9998"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.05921.net\/index.php\/wp-json\/wp\/v2\/tags?post=9998"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}